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1.
Fortaleza; s.n; 2016. 67 p. ilus.
Tese em Português | LILACS | ID: biblio-971926

RESUMO

Atualmente, a incidência de infecções fúngicas tem aumentado significativamente,contribuindo assim para morbidade e mortalidade. Esse fato que tem como umas das principais causas o aumento da resistência antimicrobiana, estando associado à produção de biofilme que tem um elevado nível de resistência antimicrobiana juntamente com o arsenal farmacológico antifúngico limitado, quando comparado ao arsenal antibacteriano, leva a necessidade do desenvolvimento de novas estratégias terapêuticas. A berberina tem demonstrado ter ampla atividade antibacteriana e antifúngica. Dentro desse contexto, o objetivo foi avaliar o potencial antifúngico da berberina frente às cepas de Candida spp. e Cryptococcus neo formans resistentes ao fluconazol, bem como avaliar o efeito da berberina frente às cepas de Candida spp...


Currently, the incidence of fungal infections has increased significantly, contributing tomorbidity and mortality. It has as one of its main causes the increase of antibiotic resistance.Recently associated with biofilm production, which has a high level of antibiotic resistance,coupled with the limited antifungal pharmacological when compared to antibacterial, it leadsto the need for the development of new therapeutic strategies. The berberine has shown tohave broad antibacterial and antifungal activity. In this context, the objective was to evaluatethe antifungal potential of berberine against strains of Candida spp. and Cryptococcusneoformans resistant to fluconazole and evaluate the effect of berberine against strains ofCandida tropicalis in biofilm formation. The evaluation of the antifungal effect wasdetermined by microdilution in broth (M27-A3) and by flow cytometry techniques, in whichthe likely mechanism of action of that compound was evaluated. For the assessment of thebiofilm, a colorimetric assay (MTT) was used to determine the susceptibility of sessile cells.Strains of Candida spp...


Assuntos
Humanos , Candida , Cryptococcus neoformans , Biofilmes , Berberina
2.
Biol. Res ; 47: 1-7, 2014. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-710931

RESUMO

BACKGROUND: Schistosomiasis is caused by helminth parasites of the genus Schistosoma. Berberine chloride (BER), an isoquinoline alkaloid, has been used in vivo for its antiparasitic, antioxidant and hepatoprotective properties. In this study, the protective effect of BER and praziquantel has been compared for the extent of schistosomiasis-induced oxidative stress in hepatic tissue of mice. RESULTS: S. mansoni was able to induce inflammation and injury to the liver, evidenced (i) by an increase in inflammatory cellular infiltrations, dilated sinusoids and vacuolated hepatocytes, (ii) by decreased levels of alanine and aspartate aminotransferases and increased levels of alkaline phosphatase, γ-glutamyl transferase in the liver homogenate, (iii) by increased production of nitric oxide and thiobarbituric acid reactive substances, and (iv) by lowered glutathione levels and decreased activities of catalase and superoxide dismutase, respectively. All these infection-induced parameters were significantly altered during BER treatment. In particular, berberine counteracted the S. mansoni-induced loss of glutathione and the activities of catalase and superoxide dismutase. CONCLUSION: Based on these results, it is concluded that berberine could ameliorate pre-existing liver damage and oxidative stress conditions due to schistosomiasis.


Assuntos
Animais , Camundongos , Berberina/uso terapêutico , Hepatopatias Parasitárias/tratamento farmacológico , Fígado/lesões , Estresse Oxidativo/efeitos dos fármacos , Esquistossomose/tratamento farmacológico , Alanina Transaminase/análise , Fosfatase Alcalina/análise , Aspartato Aminotransferases/análise , Catalase/metabolismo , Glutationa/análise , Infiltração de Neutrófilos , Óxido Nítrico/análise , Schistosoma mansoni , Superóxido Dismutase/metabolismo , Tiobarbitúricos/análise , gama-Glutamiltransferase/análise
3.
Biocell ; 36(3): 113-120, Dec. 2012. graf
Artigo em Inglês | LILACS | ID: lil-694711

RESUMO

Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in cell supernatants after berberine treatment, suggesting cell death. Furthermore, flow cytometry analysis showed that berberine could arrest HCT-8 cells at S phase in a time-dependent manner. To further investigate the apoptotic molecular mechanism, reverse transcription-polymerase chain reaction (RT-PCR) and western blotting methods were used. The up-regulated mRNA and/or protein expressions of Fas, FasL, TNF-a, caspase-3 and down-regulation of pro-caspase-3 suggest that the death receptor pathway may be involved in the apoptotic pathway induced by berberine. Decrease of Bcl-2 and increase of Bax in mRNA and/or protein expressions showed that the Bcl-2 family proteins were involved in berberine-induced apoptosis. We also found that berberine-induced apoptosis was associated with an up-regulated expressions of p53 and prohibitin (PHB), and decreased vimentin expression. These results suggest that berberine can suppress cell growth and reduce cell survival by arresting the cell-cycle and by inducing apoptosis of HCT-8 cells.


Assuntos
Humanos , Berberina/farmacologia , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/metabolismo , Apoptose , Berberina/metabolismo , Ciclo Celular , Linhagem Celular Tumoral , Citometria de Fluxo , L-Lactato Desidrogenase/metabolismo , Medicina Tradicional Chinesa , Microscopia de Fluorescência , RNA Mensageiro/metabolismo , Proteínas Repressoras/farmacologia , Fase S , Fatores de Tempo , Sais de Tetrazólio/farmacologia , Tiazóis/farmacologia , /metabolismo , Vimentina/metabolismo , /metabolismo
4.
Rev. cuba. farm ; 27(2): 134-8, jul.-dic. 1993. ilus
Artigo em Espanhol | LILACS | ID: lil-149867

RESUMO

Mahonia tenuifolia (Lindl.) Lour. ex Fedde (Berberis tenuifolia Lindl.) es una planta perteneciente a la familia Berberidaceae que crece en las regiones occidental y central de Cuba. El presente trabajo informa sobre la presencia de berberina en las partes aéreas de esta especie y el contenido de este alcaloide en los tallos


Assuntos
Alcaloides , Berberina/isolamento & purificação , Berberis aquifolium/análise , Cromatografia em Camada Delgada
5.
Bol. micol ; 6(1/2): 37-9, jul.-dic. 1991.
Artigo em Espanhol | LILACS | ID: lil-109889

RESUMO

Los principales antimicrobianos producidos por plantas son: ácido sórbico producido por Sorbus aucuparia L.; berberina por Berberis vulgaris L.; aliicina por Allium sativum L.; isotiocianato de bencilo por Tropaeolum majus L.; timol por Thymus vulgaris L. Se menciona también muchos otros antimicrobianos de menor importancia producidos por plantas


Assuntos
Anti-Infecciosos , Plantas Medicinais , Ácido Sórbico/antagonistas & inibidores , Antifúngicos/uso terapêutico , Berberina/uso terapêutico , Conservação de Alimentos , Alho , Timol/uso terapêutico
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