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Mem. Inst. Oswaldo Cruz ; 111(3): 209-217, Mar. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-777369

RESUMO

Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values≤ 125.0 µg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 µg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 µg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.


Assuntos
Animais , Antifúngicos/farmacologia , Aspergillus/química , Clima Desértico , DNA Fúngico/isolamento & purificação , Paracoccidioides/efeitos dos fármacos , Chlorocebus aethiops , Cromatografia de Fase Reversa , Sobrevivência Celular/efeitos dos fármacos , Citocalasinas/análise , Espectrometria de Massas , Cloreto de Metileno , Testes de Sensibilidade Microbiana , Filogenia , Análise de Sequência de DNA , Extração em Fase Sólida , Células Vero/efeitos dos fármacos
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