Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 74
Filtrar
Mais filtros










Filtros aplicados

Intervalo de ano de publicação
1.
Int. j. morphol ; 38(1): 13-16, Feb. 2020. tab
Artigo em Inglês | LILACS | ID: biblio-1056389

RESUMO

Plastination is currently the most important anatomical preservation technique due to the possibility of preserving bodies and organs for an indefinite period, in a dry and biosecure form, while preserving the morphological characteristics of the tissues. However, the shrinkage of the samples is also part of the plastination, perhaps becoming one of its few disadvantages. This paper presents the shrinkage caused by the classic technique of sheet plastination with polyester resin (Biodur® P40) in human brain slices, with the aim of statistically establishing the percentages of tissue shrinkage caused by this plastination protocol.


La plastinación es actualmente la técnica de preservación anatómica más importante debido a la posibilidad de preservar los cuerpos y órganos por un período indefinido, en forma seca y biosegura, al tiempo que preserva las características morfológicas de los tejidos. Sin embargo, la retracción de las muestras también es parte de la plastinación, quizás convirtiéndose en una de sus pocas desventajas. Este artículo presenta la retracción causada por la técnica clásica de plastinación de cortes con resina poliéster (Biodur® P40) en cortes de cerebro humano, con el objetivo de establecer estadísticamente los porcentajes de retracción de tejidos causados por este protocolo de plastinación.


Assuntos
Humanos , Tamanho do Órgão , Poliésteres/química , Encéfalo/anatomia & histologia , Inclusão em Plástico/métodos , Preservação de Tecido , Análise Estatística , Resinas
2.
Int. j. morphol ; 37(4): 1557-1563, Dec. 2019. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1040169

RESUMO

La plastinación es una técnica anatómica de conservación cadavérica creada en 1977 por Gunther von Hagens, en Heidelberg, Alemania, y que sustituye los líquidos biológicos y/o de fijación por acetona, para luego impregar las muestras con distintas resinas, dependiendo de la técnica de plastinación desarrollada, para finalmente llevar a cabo la polimerización de los componentes incorporados a las muestras, para obtener muestras biológicas secas y totalmente duraderas. El objetivo de este trabajo consistió en desarrollar un protocolo de plastinación de cortes con resina poliéster (Biodur® P40) en secciones de 3 mm de espesor de cerebro humano. La muestras fueron fijadas y conservadas con formalina al 10 %. Los cerebros luego fueron seccionados con una maquina cortadora de tejidos, obteniéndose láminas delgadas de 3 mm de espesor. Inmediatamente los cortes de cerebro fueron colocados en deshidratación en acetona al 100 %, a -25 ºC, durante 7 días el primer baño de acetona, y durante otros 3 días más, para el segundo baño de acetona. Una vez deshidratados los cortes, estos fueron colocados en resina poliéster Biodur® P40 y se llevó a cabo la impregnación forzada de los cortes, en cámara de vacío a temperatura ambiente (20 ºC). Una vez finalizada la impregnación forzada, se procedió a la etapa de curado, la cual en primer lugar consiste en el armado de las cámaras de curado dentro de las cuales se colocaran los cortes con resina poliéster. Las cámaras de curado fueron colocadas bajo luz UV para acelerar la polimerización del poliéster y finalizar el proceso de plastinación. Se logró desarrollar satisfactoriamente en el Laboratorio de Plastinación y Técnicas Anatómicas de la Universidad de La Frontera un protocolo de plastinación de cortes con resina poliéster, obteniendo una excelente conservación de cortes de cerebro, con diferenciación de sustancias gris y blanca, y conservación de todas las características morfológicas.


Plastination is an anatomical technique of cadaveric conservation created in 1977 by Gunther von Hagens, in Heidelberg, Germany, and that substitutes biological and / or fixation fluids with acetone, to then impregnate the samples with different resins, depending on the developed plastination technique, to finally carry out the polymerization of the components incorporated into the samples, to obtain dry and totally durable biological samples. The aim of this work was to develop a sheet plastination protocol with polyester resin (Biodur® P40) in 3 mm thick slices of human brain. The samples were fixed and preserved with 10 % formalin. The brains were sectioned with a slice cut machine, obtaining thin sheets of 3 mm thick. Immediately the slices of brain were placed in dehydration in 100 % acetone, at -25 °C, for 7 days the first acetone bath, and for another 3 more days, for the second acetone bath. Once the cuts were dehydrated, they were placed in Biodur® P40 polyester resin and the forced impregnation was carried out in a vacuum chamber at room temperature (20 °C). Once the forced impregnation was finished, the curing stage was carried out, which first consists in the assembly of the curing chambers within which the slices with polyester resin were placed. The curing chambers were placed under UV light to accelerate the polymerization of the polyester and finished the plastination process. A sheet plastination protocol with polyester resin was successfully developed in the Laboratory of Plastination and Anatomical Techniques of Universidad de La Frontera, obtaining excellent conservation of brain slices, with differentiation of gray and white substances, and conservation of all morphological characteristics.


Assuntos
Humanos , Poliésteres/química , Resinas Sintéticas/química , Encéfalo/anatomia & histologia , /métodos , Protocolos Clínicos
3.
Acta cir. bras ; 34(11): e201901101, Nov. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1054681

RESUMO

Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.


Assuntos
Humanos , Animais , Masculino , Poliésteres/farmacologia , Tendão do Calcâneo/cirurgia , Tendão do Calcâneo/efeitos dos fármacos , Carotenoides/farmacologia , Tenotomia/métodos , Hidroxibutiratos/farmacologia , Valores de Referência , Regeneração/efeitos dos fármacos , Tendão do Calcâneo/patologia , Reprodutibilidade dos Testes , Ratos Wistar , Colágeno Tipo I/análise , Colágeno Tipo I/efeitos dos fármacos , Colágeno Tipo III/análise , Colágeno Tipo III/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos
4.
Int. j. morphol ; 37(3): 1132-1141, Sept. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1012409

RESUMO

Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.


Las células madre espermatogónicas (CME) tienen capacidad de auto renovación y diferenciación esenciales para la producción de esperma a lo largo de la vida reproductiva masculina. El «scaffold¼ nanofibroso de policaprolactona / gelatina (PCL / Gel) electrohilado imita características importantes de la matriz extracelular (MEC), que puede proporcionar una técnica prometedora para la proliferación y diferenciación de CME in vitro. El objetivo del presente estudio fue investigar los efectos del «scaffold¼ nanofibroso PCL / Gel en la propagación y diferenciación de CME de ratones neonatos (mSSC). Los mSSC se aislaron enzimáticamente y las células se purificaron mediante un método de siembra diferencial y se sembraron en un «scaffold¼. Después de 2 semanas, se investigaron la viabilidad, el número y el diámetro de las colonias y la expresión de genes específicos de células espermatogónicas. Después de la propagación de mSSC, las células se cultivaron en un medio de diferenciación en el «scaffold¼ durante otras 2 semanas, y las células se analizaron mediante PCR en tiempo real. El número de colonias mSSC (P <0,01) y los niveles de expresión de los genes espermatogónicos específicos Plzf e Inga6 (P <0,01) y también los genes de diferenciación c-Kit, Tp1 y Ptm1 (P <0,05) fueron mayores en el grupo de «scaffold¼ en comparación con el control durante el período de cultivo. Concluimos que los mSSC pueden expandirse y diferenciarse en células espermátidas en un «scaffold¼ de nanofibras PCL / Gel con parámetros de desarrollo mejorados.


Assuntos
Animais , Masculino , Camundongos , Espermatogônias/citologia , Espermatogônias/metabolismo , Diferenciação Celular/fisiologia , Proliferação de Células/fisiologia , Poliésteres/química , Diferenciação Celular/genética , Sobrevivência Celular , Imunofluorescência , Proliferação de Células/genética , Tecidos Suporte , Nanofibras/química , Reação em Cadeia da Polimerase em Tempo Real , Animais Recém-Nascidos
5.
Acta cir. bras ; 34(6): e201900603, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1019263

RESUMO

Abstract Purpose To Compare the extent and intensity of adhesions formed between the intra-abdominal organs and the intraperitoneal implants of polypropylene mesh versus polypropylene/polyglecaprone versus polyester/porcine collagen used for correction of abdominal wall defect in rats. Methods After the defect in the abdominal wall, thirty Wistar rats were placed in three groups (ten animals each) for intraperitoneal mesh implant: polypropylene group, polypropylene/polyglecaprone group, and polyester/porcine collagen group. The macroscopic evaluation of the extent and intensity of adhesions was performed 21 days after the implant. Results The polypropylene group had a higher statistically significant impairment due to visceral adhesions (p value = 0.002) and a higher degree of intense adherence in relation to polypropylene/polyglecaprone and polyester/porcine collagen groups (p value<0.001). The polyester/porcine collagen group showed more intense adhesions than the polypropylene/polyglecaprone group (p value=0.035). Conclusions The intraperitoneal implantation of polypropylene meshes to correct defects of the abdominal wall caused the appearance of extensive and firm adhesions to intra-abdominal structures. The use of polypropylene/polyglecaprone or polyester/porcine collagen tissue-separating meshes reduces the number and degree of adhesions formed.


Assuntos
Animais , Masculino , Ratos , Doenças Peritoneais/etiologia , Poliésteres/administração & dosagem , Polipropilenos/administração & dosagem , Telas Cirúrgicas/efeitos adversos , Aderências Teciduais/etiologia , Colágeno/administração & dosagem , Dioxanos/administração & dosagem , Poliésteres/efeitos adversos , Polipropilenos/efeitos adversos , Teste de Materiais , Colágeno/efeitos adversos , Ratos Wistar , Parede Abdominal/patologia , Dioxanos/efeitos adversos
6.
Acta cir. bras ; 33(11): 1027-1036, Nov. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-973478

RESUMO

Abstract Purpose: To compare two suture threads, poliglecaprone 25 and nylon, used as intradermal suture for skin closure in women undergoing their first cesarean section. Methods: This is a randomized clinical trial. A total of 60 women undergoing their first cesarean section were enrolled and prospectively assessed. They were randomly allocated to group I (n=30), which received an intradermal suture with nylon 4.0 or to group II (n=30), which had an intradermal suture with poliglecaprone 25, 4.0. The main author took standardized photographs of the scar 6 months after the operation. Four independent raters, two senior obstetricians and two senior plastic surgeons (a male and a female physician from each specialty) assessed the photographs.The panelists rated the scar according to Trimbos scale, composed by the subscales hypertrophy, color and width of the scar. Results: At baseline, patients in both groups were similar regarding age and body mass index. Five patients withdraw the study, four from group and one from group II. Scars of patients from group II were significantly less hypertrophic (p=0.001), thinner (p=0.019) and had more acceptable color (p=0.019). Conclusion: The intradermal suture with poliglecaprone 25 for skin closure after cesarean incision provides better aesthetic result.


Assuntos
Humanos , Feminino , Poliésteres/uso terapêutico , Suturas , Cesárea/métodos , Técnicas de Sutura , Cicatriz , Dioxanos/uso terapêutico , Nylons , Valores de Referência , Materiais Biocompatíveis , Estudos Prospectivos , Reprodutibilidade dos Testes , Resultado do Tratamento , Estatísticas não Paramétricas , Estética
7.
Acta cir. bras ; 33(9): 792-798, Sept. 2018. graf
Artigo em Inglês | LILACS | ID: biblio-973505

RESUMO

Abstract Purpose: To evaluate the fibrosis induced by four different meshes: Marlex®, Parietex Composite®, Vicryl® and Ultrapro®. Methods: Histological cutouts of abdominal wall were analyzed with polarized light 28 days after the meshes implants and colorized by picrosirius to identify the intensity of collagen types I and III, and their maturation index. Results: When the four groups were compared, the total collagen area analyzed was bigger in groups A and D, with no difference between them. The collagen type I density was bigger in group A, with an average of 9.62 ± 1.0, and smaller in group C, with an average of 3.86 ± 0.59. The collagen type III density was similar in groups A, B and C, and bigger in group D. The collagen maturation index was different in each of the four groups, bigger in group A with 0.87, group B with 0.66, group D with 0.57 and group C with 0.33 (p = 0.0000). Conclusion: The most prominent fibrosis promotion in the given meshes was found on Marlex® (polypropylene mesh) and the Parietex Composite® (non-biodegradable polyester); the collagen maturation index was higher in the Marlex® mesh, followed by Ultrapro®, Parietex Composite® and Vicryl® meshes.


Assuntos
Animais , Poliésteres/efeitos adversos , Poliglactina 910/efeitos adversos , Polipropilenos/efeitos adversos , Telas Cirúrgicas/efeitos adversos , Colágeno/efeitos adversos , Parede Abdominal/patologia , Poliésteres/administração & dosagem , Poliglactina 910/administração & dosagem , Polipropilenos/administração & dosagem , Fatores de Tempo , Fibrose/etiologia , Fibrose/patologia , Teste de Materiais , Aderências Teciduais/etiologia , Aderências Teciduais/patologia , Colágeno/administração & dosagem , Modelos Animais , Parede Abdominal/cirurgia
8.
Braz. j. med. biol. res ; 51(5): e6754, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889074

RESUMO

Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on human dental pulp stem cell detachment. Polycaprolactone scaffolds were electrospun using a solvent mixture of chloroform and methanol. The viable cell number was determined at each tested condition. Cell morphology was analyzed by confocal microscopy after various incubation times for static cell adhesion with a high seeding density. Scanning electron microscopy images were obtained before and after perfusion for the highest flow rate tested. The wall pore shear stress was calculated for all tested flow rates (0.005-3 mL/min). An inversely proportional relationship between adhesion time with cell detachment under perfusion was observed. Lower flow rates and lower seeding densities reduced the drag of cells by shear stress. However, there was an operational limit for the lowest flow rate that can be used without compromising cell viability, indicating that a flow rate of 0.05 mL/min might be more suitable for the tested cell culture in electrospun scaffolds under direct perfusion.


Assuntos
Humanos , Polpa Dentária/citologia , Perfusão , Poliésteres , Células-Tronco/citologia , Tecidos Suporte , Adesão Celular , Técnicas de Cultura de Células
9.
Electron. j. biotechnol ; 30: 71-76, nov. 2017. graf, ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1021543

RESUMO

Background: Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results: Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions: This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid).


Assuntos
Poliésteres/metabolismo , Actinomycetales/enzimologia , Enzimas/biossíntese , Biodegradação Ambiental , Reatores Biológicos , Enzimas/metabolismo , Enzimas Imobilizadas , Fermentação
10.
Acta cir. bras ; 32(2): 98-107, Feb. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-837681

RESUMO

Abstract Purpose: To compare the polypropylene mesh (Marlex®) to Vicryl®, Parietex composite® and Ultrapro® meshes to assess the occurrence of adhesions in the intraperitoneal implantation. Methods: Sixty Wistar rats were allocated into three groups: PP+V, in which all the animals received a polypropylene and a Vicryl® mesh; PP+PC, with the implantation of polypropylene and Parietex composite® meshes and PP+UP, in which there was implantation of polypropylene and Ultrapro®. Macroscopic analysis was performed 28 days later to assess the percentage of mesh area affected by adhesion. Results: in the PP+ V group, the Vicryl® mesh showed lower adhesion formation (p=0.013). In the PP+PC, there were no differences between polypropylene and Parietex composite® (p=0.765). In the PP+UP group, Ultrapro® and polypropylene meshes were equivalent (p=0.198) . Conclusion: All the four meshes led to adhesions, with the Vicryl® mesh showing the least potential for its formation.


Assuntos
Animais , Masculino , Ratos , Poliésteres , Poliglactina 910 , Polipropilenos , Telas Cirúrgicas , Aderências Teciduais/prevenção & controle , Colágeno , Hérnia Ventral/cirurgia , Próteses e Implantes , Materiais Biocompatíveis , Distribuição Aleatória , Ratos Wistar , Modelos Animais , Parede Abdominal/cirurgia
11.
Braz. j. med. biol. res ; 50(1): e5492, 2017. graf
Artigo em Inglês | LILACS | ID: biblio-839240

RESUMO

The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method was successfully applied to the quantification of PHB in epiphytic bacteria isolated from rice roots.


Assuntos
Azospirillum brasilense/metabolismo , Citometria de Fluxo/métodos , Herbaspirillum/metabolismo , Hidroxibutiratos/metabolismo , Raízes de Plantas/microbiologia , Poliésteres/metabolismo , Microscopia de Fluorescência
12.
Arq. bras. med. vet. zootec ; 68(6): 1413-1421, nov.-dez. 2016. tab, graf, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-827931

RESUMO

The aim of this study was to evaluate the use of a malleable membrane composed of hydroxyapatite (60%) and polycaprolactone (40%) as treatment of periodontal disease experimentally induced in dogs. A bone defect of standardized dimensions was created between the roots of the third and fourth premolar of 12 dogs for periodontal disease induction. Six dogs had the defect covered by the membrane and six dogs received only standard treatment for periodontal disease, also applied to dogs in the treated group. The animals were clinically monitored during the experiment. Radiographs were taken after surgery and at 60 days after treatment initiation. Clinical attachment level was also assessed in those moments. On the 60th day, dental sample of all animals, containing tooth, defect and periodontal tissues, were harvested, fixed in formalin and analyzed by microtomography and histology. During the experimental period, the animals showed no pain and purulent discharge, however, there was dehiscence in 50% of animals and membrane exposure in five out of six animals in the treated group. Clinical attachment level showed no difference between groups. Radiographs showed radiopacity equal to the alveolar bone in both groups. The microtomography revealed that the control group had higher bone volume in the defect compared to the treated group; however, the furcation was not filled by new alveolar bone in any animal. Histological analysis revealed that junctional epithelium invasion was lighter in the control group. New bone was only observed in the apical edge of the defect in both groups. Although the composite is biocompatible and able to keep the space of the defect, it did not promote periodontal tissue regeneration within 60 days of observation.(AU)


O objetivo do presente trabalho foi avaliar a utilização de membrana moldável constituída por hidroxiapatita (60%) e policaprolactona (40%) como tratamento da doença periodontal, induzida experimentalmente em cães. Um defeito ósseo de dimensões padronizadas foi realizado entre as raízes do terceiro e do quarto pré-molares de 12 cães para indução da doença periodontal. Todos os cães receberam tratamento padrão para doença periodontal, e seis desses animais foram tratados também com a aplicação da membrana sobre o defeito. Os animais foram acompanhados clinicamente durante o experimento. Radiografias foram realizadas no pós-operatório e aos 60 dias após o início do tratamento. O nível clínico de inserção também foi avaliado nesses momentos. Aos 60 dias, a amostra dental de todos os animais contendo o dente, o defeito e os tecidos periodontais foi coletada, fixada em formol e analisada por microtomografia e histologia. Durante o período experimental, os animais não apresentaram dor e secreção purulenta, entretanto houve deiscência em 50% dos animais e exposição de membrana em cinco dos seis animais do grupo tratado. Nível clínico de inserção não apresentou diferença entre os grupos. As imagens radiográficas mostraram radiopacidade igual ao osso alveolar em ambos os grupos. A microtomografia revelou que o grupo controle apresentou maior volume ósseo no defeito em relação ao grupo tratado, no entanto, em todos os animais, a região de furca não foi preenchida por novo osso alveolar. A análise histológica revelou que a invasão por epitélio juncional foi mais discreta no grupo controle. Osso novo foi apenas observado na borda apical do defeito em ambos os grupos. Embora o compósito seja biocompatível e tenha sido capaz de manter o espaço do defeito, ele não promoveu a regeneração dos tecidos periodontais no período de 60 dias de observação.(AU)


Assuntos
Animais , Cães , Materiais Biocompatíveis/uso terapêutico , Regeneração Tecidual Guiada/veterinária , Hidroxiapatitas/uso terapêutico , Doenças Periodontais/veterinária , Modelos Animais , Poliésteres
13.
Acta cir. bras ; 31(5): 294-299, May 2016. graf
Artigo em Inglês | LILACS | ID: lil-783797

RESUMO

ABSTRACT PURPOSE: To compare fibroplasia and the resistance of the abdominal wall when polypropylene meshes and polypropylene/poliglecaprone are used. METHODS: Seventy-seven male Wistar rats were divided into three groups: Control Group (for resistance); Group E (polypropylene mesh); and Group U (polypropylene/poliglecaprone mesh). The animals in Groups E and U had a standard muscular and aponeurotic defect, with integral peritoneum, and correction with the mesh. Measurements were taken 4, 7, 14, 28 and 56 days after surgery. The resistance, and collagen density were studied. RESULTS: Resistance on the 56th day was similar in both meshes. The gain in resistance described an ascending curve for the polypropylene mesh and was irregular in the case of the polypropylene/poliglecaprone. Fibroplasia showed a gain in type I and type III collagen in both groups (p<0.001). Collagen III stabilized in the 14th day and collagen I continued to ascend. CONCLUSIONS: The gain in resistance of the polypropylene mesh is regular and ascending, whereas the polypropylene/poliglecaprone is not regular. The final resistance of both meshes is similar; the collagen density increases over time, and show the same inflammatory potential.


Assuntos
Animais , Masculino , Poliésteres/uso terapêutico , Polipropilenos/uso terapêutico , Colágeno/metabolismo , Parede Abdominal/cirurgia , Dioxanos/uso terapêutico , Hérnia Incisional/cirurgia , Período Pós-Operatório , Resistência à Tração/fisiologia , Teste de Materiais/instrumentação , Ratos Wistar , Modelos Animais , Colágeno Tipo I/metabolismo , Colágeno Tipo III/metabolismo , Hérnia Incisional/fisiopatologia , Hérnia Incisional/metabolismo
14.
Rev. bras. epidemiol ; 18(supl.2): 97-108, Out.-Dez. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-776711

RESUMO

RESUMO: A prevenção primordial é definida como a prevenção inicial de fatores de risco, por meio da adoção de comportamentos mais saudáveis. Dentro desse conceito, a American Heart Association (AHA) definiu sete métricas, baseadas em evidências, para se alcançar uma saúde cardiovascular (SCV) ideal. O objetivo deste trabalho foi avaliar a prevalência de SCV na população brasileira, segundo sexo, faixa etária e região de moradia, utilizando os dados da última Pesquisa Nacional de Saúde (PNS), de 2013. Foram avaliados, como preconizado pela AHA, de forma conjunta (número de fatores) e isolada, quatro fatores comportamentais (tabagismo, atividade física, índice de massa corporal e dieta) e três biológicos (pressão arterial, glicemia e níveis de colesterol). A população brasileira atingiu prevalências menores de 1%, de sete fatores em nível ideal. Isoladamente, 3,2% da população apresentaram a dieta em nível ideal, seguido da atividade física (23,6%) e índice de massa corporal (43,7%). A população entre 18 e 35 anos apresentou a maior prevalência de número de métricas conjuntas em nível ideal (0,5%), valor também atingido pela população geral da Região Norte. Os resultados indicam que devem ser realizados ainda maiores esforços por meio de políticas públicas de prevenção primordial para atingir metas adequadas de SCV na população brasileira.


ABSTRACT: Primordial prevention is defined as the initial prevention of risk factors, through the adoption of healthier behaviors. Within this concept, the American Heart Association (AHA) has defined seven metrics, based on evidence, to achieve ideal cardiovascular health. The aim of this study was to evaluate the prevalence of cardiovascular health in the Brazilian population, according to sex, age, and region of residence, using data from the latest National Health Survey (2013). We assessed the risk factors, as recommended by the AHA, combined (number of factors) and individually: four behavioral (smoking, physical activity, body mass index and diet) and three biological factors (blood pressure, blood glucose and cholesterol levels). The Brazilian population has reached very low prevalence (1%), for the sum of 7 factors in ideal level. Individually, 3.2% of the population consumed ideal diet, followed by physical activity (23.6%) and body mass index (43.7%). The subjects aged between 18 and 35 years showed higher prevalence of metrics combined at the optimal levels (0.5%), which was also reached by the population of the Northern region. These results indicate that greater efforts are urgent by public policies at the level of primordial prevention in order to achieve appropriate targets of cardiovascular health in the Brazilian population.


Assuntos
Animais , Celecoxib/administração & dosagem , /administração & dosagem , Articulações/metabolismo , Poliésteres/química , Polietilenoglicóis/química , Acetilação , Celecoxib/farmacocinética , /farmacocinética , Portadores de Fármacos , Géis , Cavalos , Espectroscopia de Prótons por Ressonância Magnética , Líquido Sinovial/metabolismo , Difração de Raios X
15.
Acta cir. bras ; 30(6): 376-381, 06/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-749645

RESUMO

PURPOSE: To compare the inflammatory response of three different meshes on abdominal hernia repair in an experimental model of incisional hernia. METHODS: Median fascial incision and skin synthesis was performed on 30 Wistar rats. After 21 days, abdominal hernia developed was corrected as follows: 1) No mesh; 2) Polypropylene mesh; and, 3) Ultrapro(r) mesh. After 21 days, the mesh and surrounding tissue were submitted to macroscopic (presence of adhesions, mesh retraction), microscopic analysis to identify and quantify the inflammatory and fibrotic response using a score based on a predefined scale of 0-3 degrees, evaluating infiltration of macrophages, giant cells, neutrophils and lymphocytes. RESULTS: No significant difference was seen among groups in adherences, fibrosis, giant cells, macrophages, neutrophils or lymphocytes (p>0.05). Mesh shrinkage was observed in all groups, but also no difference was observed between polypropylene and Ultrapro mesh (7.0±9.9 vs. 7.4±10.1, respectively, p=0.967). Post-operatory complications included fistula, abscess, dehiscence, serohematic collection and reherniation, but with no difference among groups (p=0.363). CONCLUSION: There is no difference between polypropylene (high-density) and Ultrapro(r) (low-density) meshes at 21 days after surgery in extraperitoneal use in rats, comparing inflammatory response, mesh shortening, adhesions or complications. .


Assuntos
Animais , Masculino , Dioxanos/uso terapêutico , Hérnia Ventral/cirurgia , Herniorrafia/métodos , Poliésteres/uso terapêutico , Polipropilenos/uso terapêutico , Telas Cirúrgicas , Hérnia Ventral/patologia , Herniorrafia/efeitos adversos , Teste de Materiais , Ratos Wistar , Reprodutibilidade dos Testes , Fatores de Tempo , Aderências Teciduais , Resultado do Tratamento
16.
Braz. dent. j ; 26(2): 93-98, Mar-Apr/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-741212

RESUMO

The aim of this study was to evaluate the influence of the poly-L-lactic acid (PLLA)-based scaffold's pore size on the proliferation and differentiation of dental pulp stem cells (DPSCs). The scaffolds were prepared in pulp chambers of 1-mm-thick tooth slices from third molars using salt crystals (150-250 µm or 251-450 µm) as porogen. DPSC (1x105 cells) were seeded in the scaffolds with different pore sizes, and cultured in 24-well plates. The cell proliferation was evaluated using the WST-1 assay after 3-21 days. Furthermore, RT-PCR was used to assess the differentiation of the DPSCs into odontoblasts, using markers of odontoblastic differentiation (DSPP, DSP-1 and MEPE). RNA from human odontoblasts was used as control. Cell proliferation rate was similar in both scaffolds except at the 14th day period, in which the cells seeded in the scaffolds with larger pores showed higher proliferation (p<0.05). After 21 days DPSCs seeded in both evaluated scaffolds were able of expressing odontoblastic markers DMP-1, DSPP and MEPE. In summary, both scaffolds tested in this study allowed the proliferation and differentiation of DPSCs into odontoblast-like cells.


O objetivo desse estudo foi avaliar a influência do tamanho dos poros de um scaffold à base de poli ácido láctico (PLLA) sobre a proliferação e diferenciação de células tronco da polpa dental (dental pulp stem cells - DPSC). Os scaffolds foram preparados dentro da câmara pulpar de discos de terceiros molares (1 mm), utilizando sal como porógeno (150-250 µm ou 251-450 µm). DPSC (1x105 células) foram semeadas nos scaffolds com diferentes tamanhos de poros e cultivadas em placas de 24 poços. A proliferação celular foi avaliada utilizando WST-1 após 3-21 dias. Além disso, RT-PCR foi utilizado para avaliar a diferenciação odontoblástica das DPSC utilizando marcadores da diferenciação odontoblástica (DSPP, DMP-1 e MEPE). RNA obtido de odontoblastos humanos foi utilizado como controle. A taxa de proliferação celular foi semelhante nos dois scaffolds avaliados, exceto no 14° dia, no qual as células cultivadas nos scaffolds com os maiores poros apresentaram uma maior taxa de proliferação (p<0,05). Após 21 dias, as DSPC cultivadas em ambos scaffolds avaliados foram capazes de expressar os marcadores odontoblásticos DMP-1, DSPP e MEPE. Em resumo, ambos scaffolds avaliados nesse estudo permitiram a proliferação e diferenciação odontoblástica das DPSC. .


Assuntos
Polpa Dentária/citologia , Poliésteres/química , Células-Tronco/fisiologia , Tecidos Suporte/química , Materiais Biocompatíveis/química , Diferenciação Celular , Proliferação de Células , Cavidade Pulpar/anatomia & histologia , Dente Serotino , Reação em Cadeia da Polimerase em Tempo Real , Propriedades de Superfície , Técnicas de Cultura de Tecidos , Engenharia Tecidual
17.
Int. j. morphol ; 33(1): 275-278, Mar. 2015. ilus
Artigo em Espanhol | LILACS | ID: lil-743797

RESUMO

La resina poliéster (P-4) se ha utilizado en varias técnicas anatómicas, gracias a su bajo costo, fácil manejo y obtención. Entre ellas la inclusión, que se realiza en capas dejando muestras relativamente opacas y de un grosor que no permite ver claramente las estructuras. También encontramos la plastinación de cortes anatómicos, técnica más sofisticada, compleja y de un alto costo económico. El objetivo fue idear un método de inclusión para cortes anatómicos, que mejore la calidad de la técnica clásica. Se utilizaron cortes anatómicos de segmento distal de miembro inferior humano y de un grosor promedio de 0,5 cm fijados con acetona y cortes de encéfalo de vacuno de igual grosor, fijados con formalina acuosa al 10%. La totalidad de las muestras son deshidratadas en acetona a -17 °C. Posteriormente se impregnan e incluyen en resina poliéster (P-4) a temperatura ambiente. Se obtuvieron muestras con una buena transparencia y solidez que permite ver en detalle muchas estructuras, similar a la obtenida con la técnica de plastinación en resina. Mediante un método relativamente fácil de realizar es posible obtener piezas anatómicas de muy buena calidad.


The polyester resin (P-4) has been used in several anatomical techniques, due to its low cost, easy handling and elicit. Among them, the inclusion, which is performed on samples leaving relatively opaque layers and an important thickness which does not allow a clear vision of the structures. There is also the plastination of anatomical slices with this polymer which although, technically sophisticated and complex has a high economic cost. The objective is to develop a method for anatomical section inclusion, improving the quality of classic technique. Distal segment of human lower limb anatomical slices were used with an average thickness of 0.5 cm fixed with acetone and bovine brain slices equal thickness, fixed with 10% aqueous formalin. All the samples are dried in acetone at -17 °C. Subsequently, they are impregnated and included on polyester resin (P-4) at room temperature. Samples were obtained with good transparency and solidity that allows observation of details of many structures, similar to that obtained with the resin plastination technique. Using this relatively easy method we can get very good quality anatomical samples.


Assuntos
Humanos , Animais , Técnicas Histológicas/métodos , Poliésteres , Resinas Sintéticas , Preservação de Tecido/métodos
18.
Braz. oral res. (Online) ; 29(1): 1-5, 2015. ilus
Artigo em Inglês | LILACS | ID: lil-777255

RESUMO

The aim of this study was to evaluate the penetration of a resin/polyester polymer-based material (Resilon Real Seal; SybronEndo Corp., Orange, USA) into simulated lateral canals, and the quality of obturations by different techniques. A total of 30 standardized simulated canals were divided into three groups according to the technique of obturation used: MS (McSpadden), SB (SystemB/Obtura II), and LC (Lateral Condensation). To analyze the penetration of the filling material, the simulated canals were digitalized and the images were analyzed using the Leica QWIN Pro v2.3 software. The data of the middle and apical thirds were separately submitted to analysis of variance (ANOVA), followed by the Tukey’s test for the comparison of the techniques. Results showed a significant difference (p < 0.05) between groups (LC < SB) in the middle third, and a significant difference (p < 0.05) between groups (LC < SB and MS < SB) in the apical third. To analyze the quality of the obturations, the canals were radiographed and evaluated by three examiners. The Kappa test on interexaminer agreement and the nonparametric Kruskal-Wallis test indicated no significant difference between filling techniques. It was concluded that Resilon achieves greater levels of penetration when associated with thermoplastic obturation techniques.


Assuntos
Cavidade Pulpar/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/química , Obturação do Canal Radicular/métodos , Análise de Variância , Cavidade Pulpar , Guta-Percha/química , Teste de Materiais , Poliésteres/química , Estatísticas não Paramétricas
19.
São Paulo; s.n; 2014. 81 p. ilus, tab, graf.
Tese em Português | LILACS | ID: lil-719950

RESUMO

INTRODUÇÃO: Os enxertos vasculares sintéticos disponíveis atualmente apresentam baixos índices de patência, quando utilizados na revascularização de vasos de pequeno calibre, e possuem resultados inferiores quando comparados ao uso de veias autólogas em derivações infrageniculares. Nova prótese de pequeno calibre confeccionada em silicone (polidimetilsiloxano, PDMS) com reforço de tecido de poliéster foi desenvolvida e comparada à prótese de PTFE. OBJETIVOS: Analisar, em modelo experimental em coelhos, o tubo de PDMS como material para prótese vascular e compará-lo a prótese de PTFE. MÉTODOS: Quarenta coelhos foram submetidos a interposição na aorta infrarrenal de próteses de 4mm de diâmetro, sendo 20 animais com PDMS e 20 com PTFE (grupo controle). Foi medido o tempo de clampeamento e realizada arteriografia retrógrada da aorta para avaliar a patência das próteses. Para avaliar a endotelização das próteses foi realizada microscopia eletrônica de maneira amostral pareada. RESULTADOS: Vinte e cinco animais (62,5%) não apresentaram intercorrências pós-operatórias; oito (20%) morreram precocemente e sete (17,5%) ficaram paraplégicos no pós-operatório imediato (e foram sacrificados), sendo que esses animais não foram incluídos nas análises de patência. Não foi observada diferença entre os grupos quanto à evolução com complicações pós-operatórias (p=0,526) e quanto ao tempo de clampeamento da aorta (p=0,299). A patência em 30 dias foi de 100% para as duas próteses. Aos 60 dias, a taxa de patência do PDMS foi de 92,3% (± 7,4), e de 73,8% (±13,1) em 90 dias; as próteses de PTFE tiveram taxas de patência de 87,5% (± 11,7) aos 60 e 90 dias. Não foi observada diferença significativa entre as taxas de patência dos grupos (p=0,62). Não houve diferença siginificativa entre os grupos quanto ao grau de estenose das próteses patentes (p=0,650) à avaliação angiográfica. A microscopia eletrônica mostrou crescimento endotelial limitado às regiões próximas...


INTRODUCTION: Synthetic vascular grafts currently available have suboptimal patency rates in small-diameter vessels and inferior outcomes in below-the-knee arterial bypass procedures when compared to the use of autologous vein. A new small vessel prosthesis made of silicone (polydimethylsiloxane, PDMS) and reinforced with polyester fabric was developed and compared to the standard PTFE prosthesis. OBJECTIVES: On a rabbit experimental model, we compared the outcomes of new PDMS vascular prostheses with PTFE vascular prostheses. METHODS: Forty rabbits underwent infra-renal aorta replacement with 4 mm diameter prostheses, twenty animals with PDMS and twenty animals with PTFE (control group). Aortic clamping time was measured and retrograde aortic angiography was performed to assess patency. Histological graft samples were examined by electron microscopy to evaluate prostheses endothelialization. RESULTS: Twenty-five (62,5%) animals had good surgical outcome; eight animals (20%) expired and seven animals (17.5%) became paraplegic (and subsequently sacrificed) during early follow up and were not included in anastomosis patency analysis. Postoperative complications (death, paraplegia) rates (p=0,526) and aortic clamping times (p=0,299) were comparable in both groups. Patency rates in 30 days were 100% for both grafts. At 60 days, patency rate for PDMS was 92,3% (±7,4), and 73,8% (±13,1) at 90 days. PTFE grafts had patency rates of 87,5% (±11,7) at 60 and 90 days. No statistically significant difference was found in between groups for patency rates (p=0,62). No statistically significant difference for stenosis was found on angiographical analysis in between groups (p=0,650). Electron microscopy revealed limited anastomotic endothelial ingrowth in both prostheses used. CONCLUSION: In this experimental model, PDMS and PTFE vascular prostheses had comparable outcomes and PDMS prosthesis could be used as a vacular graft


Assuntos
Animais , Masculino , Feminino , Adulto , Coelhos , Aorta Abdominal/cirurgia , Prótese Vascular , Estudo Comparativo , Dimetilpolisiloxanos , Implantes Experimentais , Poliésteres , Politetrafluoretileno , Coelhos , Silicones
20.
Acta cir. bras ; 29(supl.1): 45-51, 2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-720404

RESUMO

PURPOSE: To compare the inflammatory reaction and the growing resistance of the abdominal wall with the use of poliglecaprone meshes and polypropylene meshes associated with poliglecaprone in the correction of abdominal defects. METHODS: Seventy-seven Wistar rats were divided into three groups: CG (non-operated animals: EG (polypropylene mesh) and UG (polypropylene and poliglecaprone mesh). A muscular and aponeurotic defect was formed and treated according to the group. Evaluations were made after 4, 7, 14, 28 and 56 days. The resistance and inflammatory pattern were studied. RESULTS: There was a gradual and significant gain in resistance, regularly in the EG and irregularly in the UG, which was lower on the 14th day (p=0.008). The inflammatory reaction was acute and more intense in the UG on the fourth day. At all other times, the inflammatory pattern was acute to chronic, similar in both groups, with minimum intensity on the 56th day. CONCLUSION: The greater resistance offered by the polypropylene mesh was regular and ascending, stabilizing on the 28th day, while that of the polypropylene/poliglecaprone was not even. In the end, the resistances were similar. The inflammatory response was greater in the UG on the fourth day and similar at all other times. .


Assuntos
Animais , Masculino , Parede Abdominal/cirurgia , Implantes Absorvíveis/efeitos adversos , Dioxanos/efeitos adversos , Reação a Corpo Estranho/etiologia , Poliésteres/efeitos adversos , Polipropilenos/efeitos adversos , Telas Cirúrgicas/efeitos adversos , Parede Abdominal/patologia , Materiais Biocompatíveis , Fibrose , Hérnia Ventral/cirurgia , Teste de Materiais , Ratos Wistar , Fatores de Tempo , Resultado do Tratamento , Resistência à Tração/efeitos dos fármacos , Cicatrização
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA