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1.
Acta cir. bras ; 30(8): 542-550, Aug. 2015. tab, ilus
Artigo em Inglês | LILACS | ID: lil-757986

RESUMO

PURPOSE: To evaluate the effect of Black cumin (Nigella sativa Linn.) pre-treatment on renal ischemia/reperfusion (I/R) induced injury in the rats.METHODS: A total of 40 male Wistar rats were randomly allocated into five equal groups including Sham, I/R model and three I/R+ Black cumin (0.5, 1 and 2%)-treated groups. I/R groups' kidneys were subjected to 60 min of global ischemia at 37°C followed by 24 h of reperfusion. At the end of reperfusion period, the rats were euthanized. Superoxide dismutase, catalase and glutathione peroxidase activities as well as reduced glutathione and renal malondialdehyde contents were determined in renal tissues. Kidney function tests and histopathological examination were also performed.RESULTS: High serum creatinine, blood urea nitrogen and uric acid as well as malondialhehyde (MDA) levels, and low antioxidant enzyme activities were observed in I/R rats compared to the sham rats. Pre-treatment with Black cumin for three weeks prior to IR operation improved renal function and reduced I/R induced renal inflammation and oxidative injury. These biochemical observations were supported by histopathological test of kidney sections.CONCLUSION:Black cumin significantly prevented renal ischemia/reperfusion induced functional and histological injuries.


Assuntos
Animais , Masculino , Rim/irrigação sanguínea , Nigella sativa/química , Preparações de Plantas/uso terapêutico , Traumatismo por Reperfusão/prevenção & controle , Nitrogênio da Ureia Sanguínea , Creatinina/sangue , Testes de Função Renal , Rim/efeitos dos fármacos , Malondialdeído/análise , Estresse Oxidativo , Peroxidases/sangue , Preparações de Plantas/farmacologia , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Traumatismo por Reperfusão/sangue , Sementes/química , Fatores de Tempo , Resultado do Tratamento , Ácido Úrico/sangue
2.
Ciênc. saúde coletiva ; 20(1): 175-184, 01/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-733141

RESUMO

This study seeks to identify the formation of social support networks of people with physical disabilities, and how these networks can help facilitate access to health services and promote social inclusion. It is a cross-sectional study, with data collected via a form applied to physically disabled persons over eighteen years of age registered with the Family Health Teams of the municipal district of João Pessoa in the state of Paraíba. It was observed that the support networks of these individuals predominantly consist of family members (parents, siblings, children, spouses) and people outside the family (friends and neighbors). However, 50% of the interviewees declared that they could not count on any support from outside the family. It was observed that the support network contributes to access to the services and participation in social groups. However, reduced social inclusion was detected, due to locomotion difficulties, this being the main barrier to social interaction. Among those individuals who began to interact in society, the part played by social support was fundamental.


Este estudo objetiva identificar a constituição das redes de apoio social das pessoas com deficiência física e como estas podem contribuir para facilitar o acesso aos serviços de saúde e a inclusão social das mesmas. Trata-se de um estudo transversal, com dados coletados através de um formulário, aplicado em pessoas com deficiência física maiores de dezoito anos, cadastradas nas Equipes de Saúde da Família do município de João Pessoa (PB). Constatou-se que as redes de apoio dessas pessoas estão constituídas principalmente pelos componentes da dimensão familiar (pais, irmãos, filhos, cônjuges) e extrafamiliar (amigos e vizinhos). No entanto, 50% dos entrevistados relataram não contar com qualquer apoio fora da família. Verificou-se que a rede de apoio contribui para o acesso aos serviços e para a participação em grupos sociais. Evidenciou-se, porém, uma reduzida inserção social, decorrente da dificuldade de locomoção, sendo esta a principal barreira para a interação social. Entre as pessoas que começaram a interagir na sociedade o apoio social foi fundamental.


Assuntos
Antraquinonas/metabolismo , Aspergillus oryzae/metabolismo , Corantes/metabolismo , Peroxidases/metabolismo , Aspergillus oryzae/enzimologia , Microbiologia Industrial , Proteínas Recombinantes/metabolismo , Poluentes Químicos da Água/metabolismo
3.
Clinics ; 69(10): 677-682, 10/2014. tab
Artigo em Inglês | LILACS | ID: lil-730467

RESUMO

OBJECTIVES: To determine the serum and tissue levels of markers of impaired oxidative metabolism and correlate these levels with the histopathology and Alvarado score of acute appendicitis patients. METHOD: Sixty-five acute appendicitis patients (mean age, 31.4±12.06 years; male/female, 30/35) and 30 healthy control subjects were studied. The Alvarado score was recorded. Serum samples were obtained before surgery and 12 hours postoperatively to examine the total antioxidant status, total oxidant status, paraoxonase, stimulated paraoxonase, arylesterase, catalase, myeloperoxidase, ceruloplasmin, oxidative stress markers (advanced oxidized protein products and total thiol level) and ischemia-modified albumin. Surgical specimens were also evaluated. RESULTS: The diagnoses were acute appendicitis (n = 37), perforated appendicitis (n = 8), phlegmonous appendicitis (n = 12), perforated+phlegmonous appendicitis (n = 4), or no appendicitis (n = 4). The Alvarado score of the acute appendicitis group was significantly lower than that of the perforated+phlegmonous appendicitis group (p = 0.004). The serum total antioxidant status, total thiol level, advanced oxidized protein products, total oxidant status, catalase, arylesterase, and ischemia-modified albumin levels were significantly different between the acute appendicitis and control groups. There was no correlation between the pathological extent of acute appendicitis and the tissue levels of the markers; additionally, there was no correlation between the tissue and serum levels of any of the parameters. CONCLUSIONS: The imbalance of oxidant/antioxidant systems plays a role in the pathogenesis acute appendicitis. The Alvarado score can successfully predict the presence and extent of acute appendicitis. .


Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Apendicite/metabolismo , Estresse Oxidativo/fisiologia , Espécies Reativas de Oxigênio/análise , Doença Aguda , Apendicectomia , Antioxidantes/análise , Arildialquilfosfatase/análise , Biomarcadores/análise , Estudos de Casos e Controles , Hidrolases de Éster Carboxílico/análise , Estudos Prospectivos , Peroxidases/análise , Valores de Referência , Espécies Reativas de Oxigênio/metabolismo , Estatísticas não Paramétricas , Albumina Sérica/análise
5.
Braz. j. microbiol ; 45(1): 127-134, 2014. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-709465

RESUMO

Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. The enzyme was purified to homogeneity using (NH4)2SO4 precipitation, DEAE-Sepharose and Sephadex G-100 column chromatography. The final enzyme activity achieved 81UmL-1, specific activity 78 U mg-1 with purification fold of 130 and recovery 1.2% of the crude enzyme. SDS-PAGE indicated that the pure enzyme have a molecular mass of approximately 42 kDa. The optimum pH was between 4-5 and the optimum temperature was 25 ºC. The pure MnP activity was enhanced by Mn2+,Cu2+,Ca2+ and K+ and inhibited by Hg+2 and Cd+2.H2O2 at 5 mM enhanced MnP activity while at 10 mM inhibited it significantly. The MnP-cDNA encoding gene was sequenced and determined (GenBank accession no. AB698450.1). The MnP-cDNA was found to consist of 497 bp in an Open Reading Frame (ORF) encoding 165 amino acids. MnP from P. ostreatus could detoxify aflatoxin B1 (AFB1) depending on enzyme concentration and incubation period. The highest detoxification power (90%) was observed after 48 h incubation at 1.5 U mL-1 enzyme activities.


Assuntos
Aflatoxinas/metabolismo , Peroxidases/isolamento & purificação , Peroxidases/metabolismo , Pleurotus/enzimologia , Biotransformação , Precipitação Química , Cromatografia em Gel , Cromatografia por Troca Iônica , DNA Fúngico/química , DNA Fúngico/genética , Eletroforese em Gel de Poliacrilamida , Ativadores de Enzimas/metabolismo , Inibidores Enzimáticos/metabolismo , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Peso Molecular , Metais/metabolismo , Fases de Leitura Aberta , Peroxidases/química , Análise de Sequência de DNA , Temperatura
6.
Biosci. j. (Online) ; 29(5-Supplement 1): 1664-1671, nov. 2013. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-967398

RESUMO

This study had as purpose to test the hypothesis that peroxidase enzyme activity could be used as an indicator of tolerance to water deficit in soybean cultivars. The experiment was carried out in pots containing 20-L of a Rhodic Hapludox soil in greenhouse conditions. Treatments were arranged in a randomized block design in a 2 × 4 factorial: two water regimes (with and without water deficit) and four soybean cultivars (CD 204, CD 215, CD 225RR and CD 226RR), with four replications. Soybean plants were grown until the beginning of flowering (R1-R2) with soil moisture content near at the field capacity, and then, it started the differentiation of treatments under water deficit by the suspension of water supply. Changes in relative water content (RWC) in leaves, protoplasmic tolerance, peroxidase activity, and plant growth were measured after four days of drought stress and 4-d recovery. The soybean cultivars used in this study have different responses under water deficit conditions. The CD 215 cultivar was the most sensitive to water deficit in the beginning of flowering stage. Soybean cultivars presented variable responses to the peroxidase enzyme activity, and this variable can be used in the selection of soybean genotypes with greater tolerance to drought.


Este estudo teve como objetivo testar a hipótese de que a atividade das enzimas peroxidase poderia ser utilizada como um indicativo de tolerância à deficiência hídrica em cultivares de soja. O experimento foi realizado em vasos contendo 20L de um Latossolo vermelho argiloso em condições de casa-de-vegetação. Os tratamentos foram dispostos no delineamento de blocos casualizados em esquema fatorial 2 × 4, constituído de dois regimes hídricos (sem e com déficit hídrico) e quatro cultivares de soja (CD 204, CD 215, CD 225RR and CD 226RR), com quatro repetições. A soja foi cultivada até o início do florescimento com teor de água no solo próximo a capacidade de campo, e então, iniciouse a diferenciação dos tratamentos com déficit hídrico, mediante a suspensão do fornecimento de água. Alteração no conteúdo relativo de águas nas folhas, tolerância protoplasmática, atividade das enzimas peroxidade e crescimento das plantas foram avaliados após quatro dias de exposição ao déficit hídrico e após quatro dias de recuperação das plantas em condições controle. As cultivares de soja utilizadas neste estudo apresentam respostas distintas em condições de déficit hídrico. A cultivar CD 215 foi mais sensível ao déficit hídrico imposto no inicio do florescimento. As cultivares de soja apresentaram respostas variáveis à atividade das enzimas peroxidase, e esta variável pode ser utilizada na seleção de genótipos de soja com maior tolerância à seca.


Assuntos
Peroxidases , Soja , Membrana Celular , Antioxidantes
7.
Rev. argent. microbiol ; 44(2): 105-112, jun. 2012. graf, tab
Artigo em Espanhol | LILACS | ID: lil-657620

RESUMO

The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58 % for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xylidine, respectively, in 5 h.


El objetivo de este trabajo fue evaluar el potencial del escobajo, un residuo agroindustrial, como sustrato para el crecimiento y la producción de enzimas lignocelulósicas de tres hongos causantes de pudrición blanca en la madera: Trametes trogii, Stereum hirsutum y Coriolus antarcticus. Para ello se utilizaron técnicas de fermentación en estado sólido. También se ensayó la decoloración de colorantes industriales sobre estos cultivos. La pérdida de peso seco del sustrato fue similar después del día 60 (33-43 %). C. antarcticus produjo las mayores actividades de lacasa y Mn-peroxidasa (33,0 y 1,6 U/g peso seco). La mayor actividad endoglucanasa fue medida en cultivos de S. hirsutum (10,4 U/g), y la mayor actividad endoxilanasa en T. trogii (14,6 U/g). El sistema C. antarcticus/escobap mostró un importante potencial para su aplicación en la biorremediación de efluentes textiles, con porcentajes de decoloración de 93, 86, 82, 82, 77 y 58 % para índigo carmín, verde de malaquita, azure B, azul R brillante de remazol, cristal violeta y xilidina, respectivamente, en 5 h.


Assuntos
Biodegradação Ambiental , Basidiomycota/crescimento & desenvolvimento , Celulase/isolamento & purificação , Corantes/metabolismo , /isolamento & purificação , Proteínas Fúngicas/isolamento & purificação , Resíduos Industriais , Microbiologia Industrial/métodos , Lacase/isolamento & purificação , Lignina/metabolismo , Peroxidases/isolamento & purificação , Caules de Planta/microbiologia , Vitis/microbiologia , Argentina , Basidiomycota/enzimologia , Celulase/metabolismo , Corantes/classificação , Coriolaceae/enzimologia , Coriolaceae/crescimento & desenvolvimento , /metabolismo , Fermentação , Proteínas Fúngicas/metabolismo , Lacase/metabolismo , Peroxidases/metabolismo , Trametes/enzimologia , Trametes/crescimento & desenvolvimento
8.
Electron. j. biotechnol ; 13(6): 14-15, Nov. 2010. ilus, tab
Artigo em Inglês | LILACS | ID: lil-591918

RESUMO

Ligninolytic enzymes of the basidiomycetes play a crucial role in the global carbon cycle. The demand for application of ligninolytic enzymes complexes of white-rot fungi in industry and biotechnology is ever increasing due to their use in a variety of processes. Ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. This ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds and dyes. Their capacities to remove xenobiotic substances and produce polymeric products make them a useful tool for bioremediation purposes. This paper reviews the applications of ligninolytic enzymes of basidiomycetes within different industrial and biotechnological area.


Assuntos
Basidiomycota/enzimologia , Lignina , Lacase/química , Peroxidases/química , Biodegradação Ambiental , Biotecnologia , Indústria Farmacêutica , Indústria Alimentícia , Lacase/metabolismo , Manganês , Indústria de Papel e Celulose , Peroxidases/metabolismo
9.
Braz. j. microbiol ; 40(1): 31-39, Jan.-Mar. 2009. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-513112

RESUMO

Wood rotting Basidiomycetes collected in the ôEstação Ecológica do Noroeste Paulistaõ, São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat branor rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data showed an increase of 3 up to 4-fold in the laccase production compared to that obtained in SSF on wheat bran. The laccases presented optimum pH at 3.0-3.5 and were stable at neutral pH values. Optimum pH for MnP and LiP activities was at 3.5 and between 4.5 and 6.0, respectively. All the strains produced laccase with optimum activities between 55-60ºC while the peroxidases presented maximum activity at temperatures of 30 to 55ºC. The crude enzymes promoted decolorization of chemically different dyes with around 70% of decolorization of RBBR and cybacron blue 3GA in 6h oftreatment. The data indicated that enzymes from these basidiomycetes strains are able to decolorize synthetic dyes.


Fungos decompositores de madeira, do grupo Basidiomicetes, coletados na ôEstação Ecológica do NoroestePaulistaõ, São José do Rio Preto, São Paulo, Brasil, pertencentes a ordem Aphyllophorales e identificados como Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp. SXS48,Picnoporus sanguineus SXS 43 e Phellinus rimosus SXS47 foram estudados para a produção de ligninases por FES (fermentação em estado sólido) usando farelo de trigo ou palha de arroz como meio de cultura. A espécie C. byrsina produziu a maior quantidade de lacase (200 U mL-1) enquanto que Lentinus sp. foi o melhor produtor de manganês peroxidase (MnP) e lignina peroxidase (LiP) (7 e 8 U mL-1, respectivamente), quando cultivados em meio composto por farelo de trigo. A avaliação do efeito da suplementação de nitrogênio do substrato sólido lignocelulósico (palha de arroz) indicou um aumento de 3 a 4 vezes na produção de lacase. A caracterização das enzimasmostrou que as lacases apresentaram atividade ótima em pH 3,0-3,5 e foram estáveis em pH de neutro a alcalino. O pH ótimo para atividade de MnP e LiP foi de 3,5 e entre 4,5 e 6,0, respectivamente. Todas as linhagens produziram lacase com atividade ótima a 55-60ºC, enquanto as peroxidases apresentaram atividades máximas entre temperaturas de 30 e 55ºC. A aplicaçãodas soluções enzimáticas brutas, obtidas pelo cultivo das linhagens em meio de farelo de trigo, em testes de descoloração de corantes sintéticos de diferentes grupos químicos levou amais 70% de perda de cor dos corantes RBBR e de cybacron blue 3GA, em 6h de tratamento. Os dados obtidos indicaramque as soluções enzimáticas contendo ligninases produzidas pelas linhagens de basidiomicetos estudadas promoveram adescoloração de corantes sintéticos.


Assuntos
Corantes/análise , Basidiomycota/enzimologia , Basidiomycota/isolamento & purificação , Fermentação , Lacase/análise , Lacase/isolamento & purificação , Manganês/análise , Manganês/isolamento & purificação , Peroxidases/análise , Peroxidases/isolamento & purificação , Métodos , Técnicas , Madeira
10.
Araraquara; s.n; 2009. 104 p. ilus.
Tese em Português | LILACS, BBO - Odontologia | ID: lil-590739

RESUMO

Mediadores inflamatórios como mieloperoxidase (MPO) e óxido nítrico (NO) participam do processo inflamatório da doença periodontal e na associação ao diabetes. Inibidores da Óxido Nítrico Sintase (NOS), como o L-NAME têm sido administrados na tentativa de minimizar danos teciduais decorrentes da inflamação. O objetivo deste estudo foi avaliar o efeito do L-NAME sobre os níveis da isoforma induzível de óxido nítrico (iNOS), sobre a perda óssea alveolar e sobre os níveis de mieloperoxidase (MPO) em ratos diabéticos com periodontite induzida. Foram utilizados 192 ratos divididos em grupos de 24 animais cada: grupo C: Controle com ingestão de água; grupo C-L: Controle com ingestão de LNAME; grupo D: Ratos diabéticos com ingestão de água; grupo D-L: Ratos diabéticos com ingestão de L-NAME; grupo P: Ratos com periodontite experimental e ingestão de água; grupo P-L: Ratos com periodontite experimental e ingestão de L-NAME; grupo DP: Ratos diabéticos com periodontite experimental e ingestão de água; e grupo DP-L: Ratos diabéticos com periodontite experimental e ingestão de L-NAME. O sacrifício dos animais foram realizados aos 3,7,15,e 30 dias após a indução da periodontite experimental. Foram realizadas análises da taxa glicêmica, da perda óssea alveolar, da atividade de mieloperoxidase e da expressão de iNOS. Os grupos com D, P e DP mostraram níveis de iNOS estatisticamente mais altos quando comparados aos grupos D-L, P-L e DP-L que ingeriram L-NAME em todos os períodos. (p < 0.05). Os grupos que ingeriram L-NAME mostraram perda óssea estatisticamente menor quando comparados aos grupos que ingeriram água (p < 0.05). A alta expressão de MPO foi observada nos grupos com periodontite P e DP que ingeriram água. Foi verificado um pico nos níveis de MPO aos 7 dias em todos os grupos experimentais que ingeriram água...


Inflammatory mediators such as myeloperoxidase (MPO) and nitric oxide (NO) play a role in inflammatory processes related to periodontal disease and diabetes. NO-Synthase (NOS) inhibitors as L-NAME have been administered in attempts to reduce tissue damage resulting from such inflammation. The aim of this study was to evaluate the effects of L-NAME on alveolar bone loss, NO and MPO levels in diabetic rats with experimental periodontitis. A hundred ninety-two rats were divided into one of the following groups with 24 animals each: group C-W: control group with water intake; group C-L: control group with L-NAME intake; group D-W: diabetic rats with water intake; group D-L: diabetic rats with LNAME intake; group P-W: chronic periodontitis rats with water intake; group PL: chronic periodontitis rats with L-NAME intake; group DP-W: diabetic chronic periodontitis rats with water intake, and group DP-L: diabetic chronic periodontitis rats with L-NAME intake. The killing was performed at 3, 7, 15 and 30 days after ligature-periodontitis induction to obtain gingival specimens and to evaluate MPO and NO activity and radiographic bone loss. Groups with D-W or P-W and DP-W showed statistically higher iNOS expression compared to LNAME groups in all periods (p < 0.05). L-NAME treatment (L) statistically decreased iNOS expression in all groups with inflammatory pathological conditions, such as D, P and DP. In general, groups with L-NAME intake showed lower bone loss compared to water intake groups. In addition, P-L or DP-L rats demonstrated statistically lower bone loss compared to diabetics and controls at 30 days (p < 0.05). The highest MPO expression was verified in periodontitis groups with water intake (P-W e DP-W). There was a peak in MPO levels at 7 days in all experimental groups with water intake during experimental evolution. The L-NAME statistically decreased MPO levels from all groups...


Assuntos
Diabetes Mellitus , Óxido Nítrico , Doenças Periodontais , Peroxidases , Ratos
11.
Electron. j. biotechnol ; 11(4): 13-14, Oct. 2008. ilus, tab
Artigo em Inglês | LILACS | ID: lil-531921

RESUMO

Four white rot fungi (WRF) strains, Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona and Pycnoporus coccineus, were tested for efficiency of treatment of Olive Oil mill wastewaters (OOMW) in relation with their cultivation mode, i.e. under the form of free mycelium, mycelium immobilized in alginate beads and solid state cultivation on Petri dishes. Study of biodegradation of phenolic compounds, chemical oxygen demand (COD) decrease and decolourisation of OOMW have shown that Coriolopsis polyzona and Pycnoporus coccineus degradation performances were apparently only slightly affected by the cell cultivation procedures experienced here. In contrast, Phanerochaete chrysosporium and Trametes versicolor showed respectively marked preferences for solid state and alginate immobilisation procedures. Both mono and polyphenolics were reduced to different extent during incubation depending on the strain, as shown by gel filtration analysis. Final pH obtained after fungal treatment of the OOMW based medium (initial pH of 5.0) was measured in order to evaluate the possibility of releasing friendly the treated wastewater in the environment. Laboratory studies as reported here may be useful for orienting the choice of a strain for treating pollution by OOMW in a particular real situation.


Assuntos
Basidiomycota/enzimologia , Fungos/enzimologia , Phanerochaete/enzimologia , Tratamento de Águas Residuárias/métodos , Alginatos , Biorremediação (Saúde Ambiental)/métodos , Peroxidases , Gorduras Vegetais
12.
Braz. j. med. biol. res ; 40(8): 1095-1099, Aug. 2007. tab
Artigo em Inglês | LILACS | ID: lil-456805

RESUMO

The aim of the present study was to evaluate the effect of Ginkgo biloba treatment (EGb 761, 200 mg kg-1 day-1) administered from day 0 to 20 of pregnancy on maternal reproductive performance and on the maternal and fetal liver antioxidant systems of streptozotocin-induced diabetic Wistar rats. On day 21 of pregnancy, the adult rats (weighing approximately 250 ± 50 g, minimum number = 13/group) were anesthetized to obtain maternal and fetal liver samples for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total glutathione (GSH-t) determinations. The uterus was weighed with its contents. The diabetic (G3) and treated diabetic (G4) groups of rats presented significant maternal hyperglycemia, reduced term pregnancy rate, impaired maternal reproductive outcome and fetal-placental development, decreased GSH-Px (G3 = G4 = 0.6 ± 0.2) and SOD (G3 = 223.0 ± 84.7; G4 = 146.1 ± 40.8), and decreased fetal CAT activity (G3 = 22.4 ± 10.6; G4 = 34.4 ± 14.1) and GSH-t (G3 = G4 = 0.3 ± 0.2), compared to the non-diabetic groups (G1, untreated control; G2, treated). For G1, maternal GSH-Px = 0.9 ± 0.2 and SOD = 274.1 ± 80.3; fetal CAT = 92.6 ± 82.7 and GSH-t = 0.6 ± 0.5. For G2, G. biloba treatment caused no toxicity and did not modify maternal or fetal-placental data. EGb 761 at the nontoxic dose used (200 mg kg-1 day-1), failed to modify the diabetes-associated increase in maternal glycemia, decrease in pregnancy rate, decrease in antioxidant enzymes, and impaired fetal development when the rats were treated throughout pregnancy (21 days).


Assuntos
Animais , Feminino , Masculino , Gravidez , Ratos , Diabetes Mellitus Experimental/metabolismo , Ginkgo biloba/química , Fígado/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Peroxidases/análise , Gravidez em Diabéticas/metabolismo , Biomarcadores/análise , Fígado/enzimologia , Resultado da Gravidez , Taxa de Gravidez , Extratos Vegetais/farmacologia , Ratos Wistar , Estreptozocina
13.
Int. j. morphol ; 25(2): 363-366, jun. 2007. ilus
Artigo em Espanhol | LILACS | ID: lil-495929

RESUMO

The localization of peroxidase activity in different cell regions is used as a criterion for the classification of the stage of maturation of mammalian mononuclear phagocytes with a positive peroxidase reaction indicating the presence of monoblasts, promonocytes, monocytes and macrophages. In this study it was evaluated the peroxidase activity of blood mononuclear phagocytes of this turtle detected at different stages of differentiation. The present observations suggest that, in turtles, the differentiation of mononuclear phagocytes occur in the blood circulation, in contrast to animals, where only are monocytes in circulating blood and macrophage differentiation occurs in other body compartments.


La localization de la actividad de la peroxidasa en diversas regiones de la célula se utiliza como criterio para la clasificación de la etapa de maduración de fagocitos mononucleares. Una reacción positiva de peroxidasa indica la presencia de monoblastos, promonocitos, monocitos y macrófagos. En este estudio fue evaluada la actividad de la peroxidasa de los fagocitos mononucleares de la sangre de la tortuga Phrynops Hilarii detectada en diversas etapas de la diferenciación. Las actuales observaciones sugieren que, en tortugas, la diferenciación de fagocitos mononucleares ocurre en la circulación de la sangre, en contraste a los mamíferos, donde están solamente los monocitos en la sangre circulante y la diferenciación de los macrófagos ocurre en otras partes del cuerpo.


Assuntos
Animais , Sangue , Fagócitos/enzimologia , Macrófagos/enzimologia , Peroxidases/metabolismo , Tartarugas
14.
Braz. j. microbiol ; 37(2): 175-180, Apr.-June 2006. tab, graf
Artigo em Inglês | LILACS | ID: lil-432630

RESUMO

O cogumelo shiitake (Lentinula edodes) vem sendo utilizado com objetivos de prevenir e controlar doenças em humanos e em vegetais. O tratamento com o extrato aquoso de basidiocarpos do cogumelo propiciou uma redução significativa na severidade da antracnose em plantas de pepino, provocada por Colletotrichum lagenarium, e um aumento na atividade de peroxidases nos tecidos foliares. Desse modo, com o objetivo de obter moléculas de interesse agronômico destinadas à proteção de plantas, o extrato aquoso bruto de basidiocarpos de L. edodes foi fracionado inicialmente com sulfato de amônio. A fração correspondente a 40-80 por cento de saturação (p40-80) foi a mais efetiva em reduzir a antracnose em cotilédones de pepino, sendo então submetida à cromatografia de troca aniônica (CTA). Após a CTA, seis picos protéicos foram obtidos, e o pico V, contendo em média 34 por cento das proteínas presentes em p40-80, provocou o acúmulo de peroxidases nos cotilédones de pepino, bem como reduziu a antracnose. A separação eletroforética das proteínas do pico V revelou a presença de mais de uma banda no gel de poliacrilamida. Portanto, estes procedimentos resultaram na purificação parcial de elicitores presentes no basidiocarpo de L. edodes.


Assuntos
Colletotrichum , Cucumis sativus , Técnicas In Vitro , Peroxidases , Plântula , Cogumelos Shiitake , Cromatografia , Métodos
15.
An. acad. bras. ciênc ; 78(2): 241-253, June 2006. ilus, tab
Artigo em Inglês | LILACS | ID: lil-427102

RESUMO

Esse artigo descreve realizações do Programa SMolBNet (Rede de Biologia Molecular Estrutural) do Estado de São Paulo, apoiado pela FAPESP (Fundação de Apoio à Pesquisa do Estado de São Paulo). Ele reúne vinte grupos de pesquisa e é coordenado pelos pesquisadores do Laboratório Nacional de Luz Síncrotron (LNLS), em Campinas. O Programa SMolBNet tem como metas: Elucidar a estrutura tridimensional de proteínas de interesse aos grupos de pesquisa componentes do Programa; Prover os grupos com treinamento em todas as etapas de determinação de estrutura: clonagem gênica, expressão de proteínas, purificação de proteínas, cristalização de proteínas e elucidação de suas estruturas. Tendo começado em 2001, o Programa alcançou sucesso em ambas as metas. Neste artigo, quatro dos grupos descrevem suas participações, e discutem aspectos estruturais das proteínas que eles selecionaram para estudos.


Assuntos
Humanos , Biologia Computacional , Genoma/genética , Biologia Molecular , Proteínas , Brasil , Cristalografia por Raios X , Biologia Computacional/organização & administração , Órgãos Governamentais/organização & administração , Interações Hospedeiro-Parasita , Biologia Molecular/instrumentação , Biologia Molecular/organização & administração , Ressonância Magnética Nuclear Biomolecular , Peroxidases/química , Peroxidases/metabolismo , Proteínas/química , Proteínas/genética , Pesquisa , Relação Estrutura-Atividade
16.
Braz. j. med. biol. res ; 39(6): 767-772, June 2006. ilus, tab
Artigo em Inglês | LILACS | ID: lil-428268

RESUMO

The present study was designed to evaluate the time course changes in peripheral markers of oxidative stress in a chronic HgCl2 intoxication model. Twenty male adult Wistar rats were treated subcutaneously daily for 30 days and divided into two groups of 10 animals each: Hg, which received HgCl2 (0.16 mg kg-1 day-1), and control, receiving the same volume of saline solution. Blood was collected at the first, second and fourth weeks of Hg administration to evaluate lipid peroxidation (LPO), total radical trapping antioxidant potential (TRAP), and superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and catalase (CAT). HgCl2 administration induced a rise (by 26 percent) in LPO compared to control (143 ± 10 cps/mg hemoglobin) in the second week and no difference was found at the end of the treatment. At that time, GST and GPx were higher (14 and 24 percent, respectively) in the Hg group, and Cu,Zn-SOD was lower (54 percent) compared to control. At the end of the treatment, Cu,Zn-SOD and CAT were higher (43 and 10 percent, respectively) in the Hg group compared to control (4.6 ± 0.3 U/mg protein; 37 ± 0.9 pmol/mg protein, respectively). TRAP was lower (69 percent) in the first week compared to control (43.8 ± 1.9 mM Trolox). These data provide evidence that HgCl2 administration is accompanied by systemic oxidative damage in the initial phase of the process, which leads to adaptive changes in the antioxidant reserve, thus decreasing the oxidative injury at the end of 30 days of HgCl2 administration. These results suggest that a preventive treatment with antioxidants would help to avoid oxidative damage in subjects with chronic intoxication.


Assuntos
Animais , Masculino , Ratos , Antioxidantes/análise , Eritrócitos/enzimologia , Peroxidação de Lipídeos/efeitos dos fármacos , Cloreto de Mercúrio/envenenamento , Estresse Oxidativo/efeitos dos fármacos , Peroxidases/sangue , Antioxidantes/metabolismo , Biomarcadores/sangue , Doença Crônica , Modelos Animais de Doenças , Luminescência , Peroxidases/metabolismo , Ratos Wistar , Fatores de Tempo
17.
Parasitol. latinoam ; 60(3/4): 105-121, dic. 2005. ilus, tab, graf
Artigo em Espanhol | LILACS | ID: lil-460425

RESUMO

El citocromo c catalizó la oxidación de las fenotiazinas (FTZ) en presencia de peróxido de hidrógeno. La formación del radical catiónico de promazina (PZ+.) se demostró por espectrofo-tometría y por su conversión a promazina sulfóxido La dihidrolipoamida deshidrogenasa (LADH) del Trypanosoma cruzi es inhibida irreversiblemente por el sistema citocromo c/H2O2 complementado con fenotiazinas. La inactivación de la LADH del parásito varía según la estructura de las FTZ, el tiempo de incubación del sistema pro-oxidante con la LADH, y la presencia de un antioxidante supresor de radicales FTZ+. Entre las 12 FTZ ensayadas, la promazina (PZ), tioridazina (TRDZ) y trimeprazina (TMPZ) fueron las más efectivas produciendo inactivaciones de 82 por ciento,76 por ciento y 72 por ciento, respectivamente, a los 90 min de incubación. El efecto de PZ (con grupo alquilamino en la posición N 10) disminuyó por modificación de su estructura en la posición 2 (efecto inactivante de PZ > cloropromazina (CPZ) > propionilpromazina (PPZ) > trifluopromazina (TFPZ) o en la posición 10 ( efecto inactivante de PZ > TMPZ > prometazina (PMTZ).El efecto de las FTZ con sustituyente piperidinil en N 10 dependió del grupo de la posición 2 ( SCH3, en TRDZ de mayor efecto; CN, en propericiazina (PCYZ), la de menor efecto entre las FTZ estudiadas). Parece que la presencia del sustituyente piperazinil en posición N 10 no tiene función importante en el efecto inactivante de las FTZ, el cual dependió de la estructura del grupo en la posición 2. El efecto de los compuestos con Cl en posición 2 (CPZ, procloroperazina (PCP), perfenazina (PFZ)) fue mayor que el obtenido con los compuestos CF3 (TFPZ, trifluoroperazina (TFP), flufenazina (FFZ), e independiente de la estructura del sustituyente N 10.El efecto de las FTZ sobre la LADH de T. cruzi depende, por lo menos en parte, de la estabilidad de los radicales FTZ+. generados por la actividad peroxidasa. La LADH T c, en comparación con la LADH de mamífero...


Cytochrome c catalyzed the oxidation of phenothiazines (PTZ) in the presence of hydrogen peroxide. The transient formation of the promazine radical cation (PZ+.) has been demonstrated by light absorption measurements as well as by its conversión to promazine sulfoxide. Trypanosoma cruzi dihydrolipoamide dehydrogenase (LADH T c) was irreversibly inhibited by treatment with cytochrome c (cyt c)/H2O2 system supplemented with PTZ. LADH T c inactivation depended on a) The PTZ structure b) Time of incubación with the complete oxidant system c) The presence of an antioxidant that intercept free radicals. PZ, thioridazine (TRDZ) and trimeprazine (TMPZ), were the most effective systems out of twelve PTZ studied, with inactivation values of 82, 76 and 72%, respectively, after 90 min of incubation. LADH T c inactivation by PZ (with alkylamine substituent at N 10 position) decreased by its structural modification at 2 position (inactivation PZ > chlorpromazine (CPZ) > propionylpromazine (PPZ)>trifluopromazine (TFPZ)) or at N 10 position (inactivation PZ > TMPZ > promethazine (PMTZ)) PTZ activity with piperidinyl substituent at N10 position depended on the group at 2 position (TRDZ, with thiomethyl group, has high inactivating effect on LADH T c; propericyazine (PCYZ), with cyano group, is much less active). Apparently, piperazinyl substituent at the N10 position on the phenothiazine have not an important function in the compound's inactivating effect on LADH T c. The effect of PTZ with Cl at 2 position (CPZ, prochlorperazine (PCP), perphenazine (PFZ)) was higher than the effect of compounds with CF3 in the same position (TFPZ,trifluoperazine (TFP),fluphenazine (FFZ) ) independent on the structure of substituents at N10 position. Production of PTZ+. radicals was essential for LADH T c inactivation and this effect depended on the stability of these free radicals. Comparision of inactivation values for LADH T c and mammalian LADH demonstrated...


Assuntos
Animais , Di-Hidrolipoamida Desidrogenase/antagonistas & inibidores , Fenotiazinas/farmacologia , Trypanosoma cruzi , Tripanossomicidas/farmacologia , Antioxidantes/farmacologia , Citocromos c/metabolismo , Di-Hidrolipoamida Desidrogenase , Peroxidases/metabolismo , Peróxido de Hidrogênio/metabolismo , Fatores de Tempo , Trypanosoma cruzi/fisiologia
18.
Genet. mol. res. (Online) ; 2(4): 348-359, Dec. 2003.
Artigo em Inglês | LILACS | ID: lil-417595

RESUMO

Lactococcus lactis, the most extensively characterized lactic acid bacterium, is a mesophilic- and microaerophilic-fermenting microorganism widely used for the production of fermented food products. During industrial processes, L. lactis is often exposed to multiple environmental stresses (low and high temperature, low pH, high osmotic pressure, nutrient starvation and oxidation) that can cause loss or reduction of bacterial viability, reproducibility, as well as organoleptic and/or fermentative qualities. Among these stress factors, oxidation can be considered one of the most deleterious to the cell, causing cellular damage at both molecular and metabolic levels. During the last two decades, considerable efforts have been made to improve our knowledge of oxidative stress in L. lactis. Many genes involved with both oxidative stress resistance and control mechanisms have been identified; functionally they seem to overlap. The finding of new genes, and a better understanding of the molecular mechanisms of stress resistance in L. lactis and other lactic acid bacterium, will lead to the construction and isolation of stress-resistant strains. Such strains could be exploited for both traditional and probiotic uses


Assuntos
Estresse Oxidativo/fisiologia , Lactococcus lactis/metabolismo , Complexos Multienzimáticos/metabolismo , Estresse Oxidativo/genética , Genes Bacterianos/genética , Lactococcus lactis/genética , NADH NADPH Oxirredutases/metabolismo , Peroxidases/metabolismo , Recombinases Rec A/metabolismo , Sobrevivência Celular/genética , Superóxido Dismutase/metabolismo
19.
Rev. argent. microbiol ; 33(2): 59-64, abr.-jun. 2001.
Artigo em Espanhol | LILACS | ID: lil-332500

RESUMO

Degradation of yard wastes by Coprinus truncorum growing in a vertical aereated bioreactor or in flasks was studied. There was a constant decay of reducing sugars in the medium that avoided their accumulation and their possible repression of degradative enzymes. Endoxylanase activity at first showed a similar pattern in both culture conditions, with maximal activity on the 12th day, but flasks maintained a high activity thereafter. Flasks also showed a higher endoglucanase activity with a peak on the 18th day, whereas the maximal value in the bioreactor was reached on the 26th day. No Mn-peroxidase and only low values of laccase activity were found. The measurements of pH and soluble proteins during the incubation period were suitable indicators of the degradation process by C. truncorum.


Assuntos
Reatores Biológicos , Coprinus , Eliminação de Resíduos/métodos , Micologia , Plantas , Biodegradação Ambiental , Carboidratos , Celulase , Celulose , Coprinus , Resíduos de Alimentos , Concentração de Íons de Hidrogênio , Lignina , Oxirredução , Oxirredutases , Peroxidases , Proteínas Fúngicas/metabolismo , Solubilidade , Temperatura , Xilosidases
20.
Biocell ; 25(1): 1-9, Apr. 2001.
Artigo em Inglês | LILACS | ID: lil-335891

RESUMO

The aim of this work was to study the physiological mechanisms of dormancy and sprouting during post-harvest of garlic (Allium sativum L.) microbulblets produced by meristem culture of garlic seed cloves. The morphological changes occurring in garlic microbulblets were assessed from harvest till sprouting in relation with peroxidase activity and levels of gibberellins. Also the effect of a cold treatment (30 days at 4 degrees C) given 30 days after harvest was studied. The results showed that during the state of dormancy in garlic microbulblets formation of the leaf primordia and vascular differentiation of the storage leaf occurred, while increases of peroxidase activity and low levels of GA3 (the only active gibberellin identified) were found. At the end of dormancy the sprouting channel was formed, vascular differentiation established, and peaks of soluble peroxidase activity as well as of GA3 were observed. At day 90 post-harvest, garlic microbulblets showed physiologically mature and able to sprout. Further on, bud expansion and decrease of GA3 levels characterized sprouting of the microbulblets. The cold treatment enhanced GA3 levels and anticipated the sprouting process.


Assuntos
Alho , Giberelinas , Peroxidases , Estruturas Vegetais , Plantas Medicinais , Diferenciação Celular , Células Cultivadas , Alho , Cromatografia Gasosa-Espectrometria de Massas , Folhas de Planta , Estruturas Vegetais
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