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Arq. bras. endocrinol. metab ; 47(6): 711-715, dez. 2003. tab
Artigo em Inglês | LILACS | ID: lil-356035


PURPOSES: To estimate plasma 1,5-anhydroglucitol (AG) in diabetic (DM) and non-DM patients in a Chinese population, and to compare it with fructosamine, glycosylated hemoglobin (HbA1c), and fasting glucose (FG) levels. METHODS: Case-control study on the significance of AG conducted in a medical center of southern Taiwan, including356 patients (300 non-DM and 56 type 2 DM). Plasma AG, fructosamine, HbA1c and FG were measured on the second day of admission and only those with normal values (except glucose) were enrolled. Glycemic markers of the non-DM patients were examined only once whereas DM patients were sequentially sampled over 3 months. RESULTS: Mean plasma AG levels were lower in DM than in non-DM patients (4.02+/-2.96 vs 26.68+/-11.33µg/ml, P<0.001), and lower in non-DM females than males (22.90+/-9.51 vs 29.45+/-11.7µg/ml, P<0.05). AG showed a good correlation with FG. Mean plasma AG were inversely correlated with FG, fructosamine and HbA1c in DM patients and worked as well as other glycemic markers in detecting short-term changes in glycemic control. AG levels of DM patients demonstrated no difference with or without smoking, hypertension, micro- and macro-vascular complications. CONCLUSIONS: We recommend clinical application of plasma AG in long-standing DM patients for short-term detection and monitoring glycemic condition.

Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Desoxiglucose , Diabetes Mellitus Tipo 2 , Glicemia , Estudos de Casos e Controles , Frutosamina , Hemoglobina A Glicada
Biol. Res ; 36(2): 155-169, July 2003. ilus, tab
Artigo em Inglês | LILACS | ID: lil-351358


Topographic distributions and laminar pattern of cortico-cortical projections from the primary auditory field (AI), anterior auditory field (AAF), dorsoposterior field (DP), ventroposterior field (VP), dorsal field (D) and ventral field (V) were studied in relation to tonotopic maps in combined anatomical, electrophysiological and 2-deoxyfluoro-D-glucose (2DG) experiments. Distributions of axons were examined by means of retrogradely-transported fluorescent tracer Fast Blue (FB) injected in the primary (AI) and anterior (AAF) auditory field. Injections of fluorescent tracer were placed in electrophysiologically-identified locations of AI and AAF. Neurons in AAF, DP, VP and V project to AI in the ipsilateral hemisphere. This area also receives projections from AI, AAF and D from the contralateral hemisphere. In AI, DP and VP, neurons are connected with AAF in the ipsilateral hemisphere and AI and AAF in the opposite hemisphere. In all cases, patches of labeling are distributed along 2DG bands oriented parallel to the isofrequency line. Substantial numbers of retrogradedly labeled neurons with similar best frequencies (BFs) were observed in the ipsilateral and moderate to scant numbers in the contralateral hemisphere. In general, regions near the injection sites receive more densely-labeled projections than do more distant targets. In both hemispheres, the supragranular layer III contains the greatest concentration of cortico-cortical cells bodies; the granular and infragranular layer V contains a somewhat lower concentration

Animais , Masculino , Feminino , Córtex Cerebral , Dominância Cerebral , Gerbillinae , Vias Neurais , Mapeamento Encefálico , Córtex Cerebral , Desoxiglucose , Eletrofisiologia , Corantes Fluorescentes , Gerbillinae , Vias Neurais
Arq. neuropsiquiatr ; 60(2A): 198-203, June 2002. ilus, tab
Artigo em Inglês | LILACS | ID: lil-309209


Status epilepticus (SE) is a medical emergency and it is associated to brain damage. 2-deoxy-[14C] glucose (2-DG) procedure has been used to measure the alterations in the functional activity of the brain induced by various pharmacological and toxicological agents. The aim of this study was to determine which changes occur in the seizure anatomic substrates during the SE induced by pilocarpine (PILO) using [14C]-2 deoxyglucose functional mapping technique. Wistar male adult rats were submitted to SE PILO-induced for 6h and received [14C] 2-deoxyglucose injection via jugular vein 45 min before the 6th hour of SE. The control animals were submitted to all procedures but received saline and not pilocarpine. Brain sections were prepared and exposed X-ray film about seven days. The optical density of each region was obtained using a solid state digital analyser. The analysis revealed that 14C-2DG utilisation was pronounced in the SE rats on the areas corresponding to the hippocampal formation (+50.6 percent), caudate-putamen (+30.6 percent), frontoparietal cortex (+32.2 percent), amygdala (+31.7 percent), entorrinal cortex (+28.2 percent), thalamic nucleus (+93.5 percent), pre-tectal area (+50.1 percent) and substantia nigra (+50.3 percent) when compared to control. Our results suggest that the different activation levels of the distinct structures may be particularly important for understanding triggering and spreading mechanisms underlying epileptic activity during status epilepticus

Animais , Masculino , Ratos , Encéfalo , Glucose , Convulsões , Estado Epiléptico , Antimetabólitos , Autorradiografia , Desoxiglucose , Modelos Animais de Doenças , Metabolismo Energético , Agonistas Muscarínicos , Pilocarpina , Ratos Wistar , Estado Epiléptico
Säo Paulo; s.n; 2001. [95] p. ilus, tab.
Tese em Português | LILACS | ID: lil-297260


O presente trabalho teve por objetivo investigar em ratos normais ntroles) e obesos pelo tratamento neonatal com glutamato monossódico (MSG): 1º - Se os glicoreceptores centrais eram sensíveis à glicoprivaçao locada pela injeçao intracerebroventricular (icv) de 2 deoxi-D-glicose na dose de 10mG/10mL/5minutos. Nestas condiçoes foi também avaliada a resposta reflexa da ingestao de alimentos, da concentraçao plasmática de glicose e da mobilizaçao dos ]os graxos nao esterificados (NEFA). Os resultados mostraram que ratos MSG am menos e ingerem menor quantidade de alimentos entre 17 e 23 horas comparado aos ratos Controle. A injeçao icv de 2 DG promoveu, nos ratos controle, lento significativo do consumo alimentar (p<0,001)enquanto nos ratos MSG nao se erva resposta. Nos ratos Controle a injeçao icv de 2 DG induziu aumento da concentraçao plasmática de glicose, enquanto nos ratos MSG em nenhum momento a glicemia diferiu do valor basal. A injeçao icv de 2 DG promoveu aumento acentuado e progressivo da concentraçao plasmática de NEFA, nos ratos Controle. Os ratos MSG apresesentaram valores basais de NEFA significativamente (p<0,001) mais elevados do os Controle mas após injeçao icv de 2 DG nao houve resposta significativa. Podemos concluir que a falta de resposta reflexa do aumento da estao de alimentos, da glicemia e da mobilizaçao de NEFA induzidos pela injeçao de 2 DG, nos ratos MSG, é decorrente da lesao central provocada pela administraçao neonatal de glutamato monossódico comprometendo a estimulaçao dos receptores envolvidos neste reflexo. 2º - Se também os glicoreceptores periféricos eram sensíveis a glicoprivaçao . Para isto foi utilizada injeçao intravenosa (i.v.) de 2 deoxi-D-glicose (2 na dose de 100mG/Kg de peso corpóreo e avaliada a resposta reflexa da ingestao alimentos, da concentraçao plasmática de glicose e ácidos graxos nao esterificados (NEFA). Os resultados mostraram que a injeçao i.v. de 2 DG, em ambos grupos experimentais, nao promoveu ingestao de alimentos. Igualmente em ambos grupos ocorreu elevaçao significativa(p<0,001 da glicemia sendo que nos ratos MSG...

Desoxiglucose , Neostigmina , Norepinefrina , Obesidade , Glutamato de Sódio
Braz. j. med. biol. res ; 27(5): 1249-1253, May 1994.
Artigo em Inglês | LILACS | ID: lil-319799


We determined the response of glucose transport to insulin in isolated adipocytes and the lipogenic activity of insulin in fragments of epididymal adipose tissue obtained from male MSG-obese rats. Basal glucose transport rates (pmol 3 min-1 10(5) cells-1) were 100 higher in MSG than in control cells (3-month old male Wistar rats) pre-incubated for 30 min (P < 0.01). Nevertheless, when expressed as fmol 3 min-1 microns 2 cell surface area-1, transport rates were similar for the two groups (31.2 +/- 2.6 for MSG and 26.5 +/- 3.2 for controls, N = 7). No differences were observed in maximally insulin-stimulated glucose transport rates between groups (72.6 +/- 10.6 for MSG and 101.0 +/- 12.0 for controls, N = 7). In contrast, for adipocytes pre-incubated for 2 h, the basal uptake rates were 3.7 times higher and the maximal response to insulin was 103 higher in cells from MSG rats compared to control cells. These alterations in MSG rat adipocytes were accompanied by changes in cell sensitivity to insulin (EC50, 0.13 +/- 0.02 ng/ml for MSG vs 0.46 +/- 0.10 ng/ml for controls, P < 0.01). The rates of incorporation of labelled substrates (3H2O and 14C-glucose) into total lipids showed that in vitro lipogenesis was also 79 (3H2O) and 250 (14C-glucose) higher in MSG adipose tissue fragments. The MSG animals were consistently hyperinsulinemic. These data suggest that the obesity of 3-month old MSG rats is a metabolic alteration characterized by an enhanced adipocyte capacity to transport glucose and to synthetize lipids resulting in increased insulin sensitivity.

Animais , Masculino , Ratos , Tecido Adiposo , Glucose , Insulina , Resistência à Insulina/fisiologia , Glutamato de Sódio , Adipócitos , Tecido Adiposo , Animais Recém-Nascidos , Desoxiglucose , Epididimo , Insulina , Ratos Wistar
Braz. j. med. biol. res ; 27(4): 995-1000, Apr. 1994.
Artigo em Inglês | LILACS | ID: lil-319816


Isolated adipocytes from rats submitted to four weeks of ad libitum feeding (AL) or meal feeding (MF, 2 h/22 h, feeding/fasting, meal time: 8:00-10:00 a.m.) schedules or pre-incubated with or without melatonin (0, 1 nM, 10 nM, 100 nM) for 5 h were submitted to insulin-stimulated [3H]-2-deoxyglucose (0.1 mM, 0.12 microCi) uptake rate measurements and insulin binding assays. Insulin sensitivity was defined as the hormone concentration capable of producing the half-maximal transport rate. Insulin sensitivity varied depending on the previous conditions of the adipocytes. In MF animals, adipose cells were more sensitive (EC50 = 0.175 ng/ml) just at the moment of the expected meal. In AL rats, sensitivity was lower (EC50 = 0.678 ng/ml) at 8:00 a.m. and increased (EC50 = 0.398 ng/ml) at 4:00 p.m. These data clearly implicate the expectation of food and period of the day with the regulation of insulin action. All these modifications in sensitivity occurred without any change in insulin receptor number or affinity. Melatonin, a secretory product of the pineal gland, induced an increase in cell sensitivity to insulin in adipocytes incubated with the highest hormone concentration (100 nM). We conclude that factors related to feeding training and circadian rhythmicity modulate cell sensitivity to insulin.

Animais , Ratos , Adipócitos , Ingestão de Alimentos/fisiologia , Insulina , Melatonina , Periodicidade , Adipócitos , Desoxiglucose , Receptor de Insulina , Resistência à Insulina/fisiologia , Fatores de Tempo
Braz. j. med. biol. res ; 26(3): 327-31, Mar. 1993. tab
Artigo em Inglês | LILACS | ID: lil-148697


The alimentary and glycemic responses to cytoglycopenia were studied in thirty-one Nile tilapia alevins of indeterminate sex and age, measuring on average 10.67 +/- 0.82 cm. The cytoglycopenia was provoked by ip injection of 60 mg/kg 2-deoxy-D-glucose (2-DG, N = 16). The control group (N = 15) was submitted to ip injection of 0.2 ml saline. Blood samples for glucose determination were obtained before and three hours after drug administration by cardiac puncture. Food was then offered ad libitum. One hour later the animals were sacrificed and their stomachs removed. The difference in wet weight between full and empty stomach was utilized to quantify the food intake. Median food intake was 0.3877 g for the fish treated with 2-DG and 0.107 g for the animals injected with saline. This difference was statistically significant by the Mann-Whitney test (P < 0.05). The median values of blood glucose levels before drug injection were 46.19 mg/100 ml in the 2-DG-treated fish and 44.54 mg/100 ml in the control group. Three hours after drug administration, the values were 48.64 mg/100 ml in the experimental group and 56.90 mg/100 ml in the control group. The difference between the values of blood glucose before and after the drug was not significant for either group. We conclude that glucoprivation provokes food intake in fish and that the same glucoprivation was not sufficient to provoke hyperglycemia

Animais , Glicemia/análise , Comportamento Alimentar , Desoxiglucose/farmacologia , Peixes/fisiologia , Peixes/sangue