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1.
Alerta (San Salvador) ; 3(2)30 abril 2020. ilus, tab
Artigo em Espanhol | LILACS | ID: biblio-1095895

RESUMO

No hay tratamiento antiviral específico para el COVID-19. Sin embargo, conocimientos adquiridos durante los brotes del SARS y el MERS, en conjunto con la información obtenida con COVID-19, han permitido detectar varios objetivos terapéuticos en el ciclo de replicación del virus, y en su patogénesis. Se incluye la evidencia actual con respecto a los principales tratamientos propuestos para COVID-19, reutilizados o experimentales, mediante una revisión de la literatura científica a la fecha. Debido a la falta de ensayos controlados aleatorios, se incluyeron: informes de casos, series de casos y artículos de revisión. Globalmente se están llevando a cabo múltiples estudios con el fin de identificar agentes que sean efectivos ante COVID-19, en los siguientes objetivos estratégicos: inhibición de la entrada/fusión del virus (anticuerpos neutralizantes, inhibidores de proteasa de serina transmembrana 2, cloroquina, hidroxicloroquina y umifenovir); interrupción de la replicación viral (remdesivir, favipiravir, lopinavir/ritonavir e ivermectina) y supresión de la respuesta inflamatoria excesiva (corticosteroides, tocilizumab, e inmunoglobulina). Aún no existe un tratamiento efectivo y seguro contra COVID-19; los fármacos descritos en esta revisión se administran como uso compasivo de drogas, o bien, como parte de un ensayo clínico. La terapia de soporte continúa siendo el pilar del manejo de COVID-19.(AU)


There is no specific antiviral treatment for COVID-19. However, knowledge acquired during the SARS and MERS outbreaks, together with the information obtained with COVID-19, have allowed the detection of various therapeutic targets in the virus replication cycle, and in its pathogenesis. The current evidence regarding the leading treatments proposed for COVID-19, reused or experimental, is included through a review of the scientific literature to date. Due to the lack of randomized controlled trials, the following were involved: case reports, case series and review articles. Globally, multiple studies are being carried out in order to identify agents that are effective against COVID-19, upon the following strategic objectives: inhibition of viral entry/fusion (neutralizing antibodies, transmembrane serine protease 2 inhibitors, chloroquine, hydroxychloroquine, and umifenovir); interruption of viral replication (remdesivir, favipiravir, lopinavir/ritonavir and ivermectin), and suppression of excessive inflammatory response (corticosteroids, tocilizumab, and immunoglobulin). There is still no effective and safe treatment against COVID-19; the medications described in this review are given as compassionate drug use, or as part of a clinical trial. Support therapy continues to be COVID-19 management cornerstone.(AU)


Assuntos
Humanos , Interleucinas/uso terapêutico , Infecções por Coronavirus/tratamento farmacológico , Lopinavir/uso terapêutico , Hidroxicloroquina/uso terapêutico , Combinação de Medicamentos
2.
An. bras. dermatol ; 94(6): 658-663, Nov.-Dec. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1054887

RESUMO

Abstract Background: Palmoplantar pustulosis is considered to be a localized pustular psoriasis confined to the palms and soles. Mutation of the IL36RN gene, encoding interleukin-36 receptor antagonist (IL-36Ra), is associated with generalized pustular psoriasis, but IL36RN mutations in Chinese palmoplantar pustulosis patients have not previously been investigated. Objective: The aim of this study was to evaluate the mutation of IL36RN in Chinese patients with palmoplantar pustulosis. Methods: Fifty-one Han Chinese patients with palmoplantar pustulosis were recruited. All exons and exon-intron boundary sequences of IL36RN were amplified in polymerase chain reactions, and Sanger sequencing of the amplicons was performed. Results: Among the 51 palmoplantar pustulosis patients, four different single-base substitutions were identified in nine patients. The mutations were c.140A>G/p.Asn47Ser in five patients, c.258G>A/p.Met86IIe in two patients, and c.115+6T>C and c.169G>A/p.Val57IIe in one patient each. All mutations were heterozygous. Comparison with the human genome database and reported literature suggested that these variants may not be pathogenic mutations causing palmoplantar pustulosis. Furthermore, there was no difference in disease severity, onset age, or disease duration between patients with these heterozygous IL36RN variants and those without (p > 0.1). Study limitation: Lack of the further evaluation of IL36Ra protein in palmoplantar pustulosis lesions. Conclusions: The four variants of IL36RN identified did not appear to be associated with the specific phenotypes of palmoplantar pustulosis.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Psoríase/genética , Interleucinas/genética , Mutação , Fenótipo , Psoríase/patologia , China , Análise de Sequência de DNA , Estatísticas não Paramétricas , Grupo com Ancestrais do Continente Asiático/genética , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Estudos de Associação Genética , Dermatoses do Pé/genética , Dermatoses do Pé/patologia , Dermatoses da Mão/genética , Dermatoses da Mão/patologia , Heterozigoto
3.
Acta cir. bras ; 34(5): e201900506, 2019. tab
Artigo em Inglês | LILACS | ID: biblio-1010876

RESUMO

Abstract Purpose: To evaluate the serum variations of Interleukins (Il) and CPR of abdominoplasties in post-bariatric patients and, to equate the homeostasis (HOMA) from the variations of glycemia and insulin to evolute the metabolic modifications. Methods: Fourteen women were submitted to abdominoplasties with weight loss after a gastroplasty. Levels of IL4, IL6, IL10, CRP, glycemia and insulin were obtained during the pre-operative, trans-operative, 24 hours post, 7th and 14th postoperative days. Results: The IL4 was higher at 24 hours post-surgery, and after a moderate decrease, it remained high until the 14th day. The IL6 and CRP had an expressive increase during the trans-operative period. The CRP remained high, and the IL6 decreased on the 7th and 14th days. The IL10 increased during the transoperative period, and it posteriorly decreased to lower levels in comparison to the pre-operative period. The already increased glycemia during the pre-operative period was even higher during the trans-operative and then, returned to preliminary values on the 7th and 14th days after surgery. The HOMA accompanied the insulin. Conclusion: The inflammatory and glycemic serum levels decrease after abdominiplasty in obese post-bariatric patients.


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Glicemia/análise , Proteína C-Reativa/análise , Interleucinas/sangue , Cirurgia Bariátrica/métodos , Abdominoplastia/métodos , Insulina/sangue , Período Pós-Operatório , Valores de Referência , Fatores de Tempo , Estudos Prospectivos , Período Pré-Operatório , Homeostase
4.
Belo Horizonte; s.n; 2019. 130 p. ilus, tab.
Tese em Inglês, Português | LILACS, BBO - Odontologia | ID: biblio-1016413

RESUMO

A maioria das resinas compostas apresenta metacrilatos como principais monômeros em sua composição. A liberação de monômeros de metacrilato, associada aos produtos de polimerização, tem sido considerada como fonte de uma série de reações biológicas como toxicidade ou reações pulpares. Os objetivos deste estudo foram avaliar o desempenho clínico de restaurações em LCNC com resina composta considerando-se também a presença de citocinas IL-1ß e IL-6 no fluido crevicular gengival e a liberação de componentes resinosos para a saliva. Utilizou-se o sistema restaurador FL-Bond II, (sistema adesivo) / Beautifil Bulk (resina composta restauradora). Foi feito um estudo clínico longitudinal in vivo, no qual foram selecionados pacientes que apresentavam uma lesão cervical não cariosa com necessidade restauradora. Dentes anteriores e posteriores com LCNC e sensibilidade foram designados como grupo experimental e o dente correspondente como grupo controle. Previamente ao tratamento, houve avaliação periodontal, coleta de saliva e de fluido crevicular gengival (FCG). As restaurações foram confeccionadas e, após 10 minutos, 7 dias, 1 mês e 6 meses foi realizada avaliação clínica das mesmas de acordo com o critério Federal Dentist International (FDI) e da resposta periodontal. Adicionalmente, em todos estes períodos de avaliação houve coleta de saliva e de fluido crevicular. As amostras de saliva foram analisadas por LC-EM a fim de identificar eventual presença dos monômeros Bis-GMA e TEGDMA. As amostras de fluido crevicular foram analisadas utilizando-se método ELISA para identificação e quantificação de interleucinas. Para realizar as comparações inter e intragrupo dos parâmetros clínicos foi utilizado o teste de McNemar para as variáveis categóricas e o teste de Wilcoxon para as variáveis numéricas. Para comparar a classificação do critério FDI entre os tempos foi utilizado o teste de Stuart-Maxwell. Para a análise da saliva, os dados de quantidade de monômeros liberados ao longo do tempo foram submetidos ao teste t de Student e a relação com quantidade total liberada foram correlacionados com o número de restaurações e com o volume total coletado de cada restauração por meio da Correlação de Pearson. O desempenho clínico das restaurações de LCNCs de um sistema restaurador resinoso bulk- fill por meio dos critérios FDI foi considerado satisfatório ao longo de 6 meses. Os parâmetros clínicos periodontais Sangramento a Sondagem, Ìndice Gengival e Profundidade de Sondagem foram mais pronunciados em torno dos dentes restaurados. O material restaurador resinoso bulk-fill não causou alteração estatisticamente significante no volume de FCG e as citocinas ao redor de dentes restaurados na amostra avaliada. Não detectou-se Bis-GMA nas amostras de saliva coletadas antes, 01 e 06 meses após. A liberação de TEGDMA média antes da restauração foi estatisticamente menor do que após 10 minutos. Os resultados do presente estudo sugerem que a restauração do LCNC pode afetar os parâmetros clínicos periodontais, porém não foi capaz de afetar a liberação de citocinas e o volume de FCG. A liberação de Bis-GMA não foi considerada significante ao longo de 6 meses, entretanto a liberação de TEGDMA foi expressiva apenas 10 minutos após a execução da restauração.


Most composite resins present methacrylates as the main monomers of their composition. In composite resins, the release of methacrylate monomers, associated with the polymerization products, has been considered as the source of a series of biological reactions such as toxicity or pulp reactions. The objectives of this study were to evaluate the clinical performance of NCCL restorations with composite resin, to determine also the presence of IL-1ß and IL-6 cytokines in the gingival crevicular fluid (GCF) and the release of resinous components to saliva. The FL-Bond II restorative system (adhesive system) / Beautifil Bulk (restorative composite resin) was used. A longitudinal clinical study was performed in vivo, where patients with a non-carious cervical lesion with a restorative need were selected. Anterior and posterior teeth with NCCL and sensitivity were designated as experimental group and the corresponding tooth as control group. Prior to the treatment, were performed periodontal evaluation, collection of saliva and gingival crevicular fluid. The lesions were restored and, after 10 minutes, 7 days, 1 month and 6 months, there was a clinical evaluation of the restorations according to the FDI criteria and the periodontal response were made. Additionally, in all of these evaluation periods there was collection of saliva and crevicular fluid were colleted. Saliva samples were analyzed by LC-MS in order to identify the possible presence of monomers. Crevicular fluid samples were analyzed using the ELISA method for identification and quantification of interleukins. To perform intra- and inter group comparisons of clinical parameters, the McNemar test for categorical variables and the Wilcoxon test for numerical variables were used. To compare the classification of the FDI criterion between the times, the Stuart-Maxwell test was used. For the analysis of saliva, the data of quantity of monomers released over time were submitted to Student's t-test and the relation with total amount released were correlated with the number of restorations and with the total volume collected from each restoration by means of Pearson's Correlation. The clinical performance of NCCL restorations of a bulk-fill resin restorative system by FDI criteria was considered satisfactory over 6 months. Considering the periodontal response of the surrounding tissue to the NCCL restorations, it was observed that periodontal clinical parameters Bleeding on Probing, Gingival Index and Prohibing on Depth were more pronounced around the restored teeth. The bulk-fill resin restorative material did not cause statistically significant changes in the volume of GCF and in the IL-1ß and IL-6 cytokines around restored teeth in the evaluated sample. No Bis-GMA was detected in the saliva samples collected before, 01 and 06 months after. For the Bis-GMA, there was no statistical difference between the analyzed periods. The mean TEGDMA release before the restoration was statistically lower than after 10 minutes. The results of the present study suggest that NCCL restoration may affect periodontal clinical parameters, but it was not able to affect the release of cytokines and the volume of GCF. The release of Bis-GMA was not considered significant over 6 months. However, the release of TEGDMA was significant only 10 minutes after the restoration was performed.


Assuntos
Adulto , Abrasão Dentária , Erosão Dentária , Teste de Materiais , Interleucinas , Resinas Compostas , Atrito Dentário , Cimentos Dentários , Esmalte Dentário , Metacrilatos
5.
Acta cir. bras ; 34(12): e201901206, 2019. tab
Artigo em Inglês | LILACS | ID: biblio-1054688

RESUMO

Abstract Purpose To evaluate the effects of prednisolone against sodium diclofenac both with ciprofloxacin compared to artificial tears on the symptoms and signs of acute viral conjunctivitis. Methods Study included 37 patients diagnosed with acute conjunctivitis and distributed by three groups: A (1% prednisolone acetate + ciprofloxacin (0.3%); B (Sodium diclofenac (0.1%) + ciprofloxacin (0.3%) and C (artificial tears + ciprofloxacin (0.3%). Patients received medication 6/6 hours daily. Signs and symptoms (e.g. lacrimation, burning, photophobia, etc.) were scored at baseline and on the first, third, fifth and seventh days and in the end of treatment using a standardized questionnaire and slit lamp anterior segment examination. Results All three groups demonstrated an improvement in the signs and symptoms of conjunctivitis in their follow-up visits. There was no significant difference in symptom and sign scores between Group A and B and B and C in the study visits ( p >0.05). However, the comparison between groups A and C showed a clinical trend (p=0.05) on third evaluation suggesting better clinical action using the corticosteroids. Conclusion The prednisolone acetate was not superior to the use of sodium diclofenac or artificial tears in relieving the signs and symptoms of viral conjunctivitis.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Prednisolona/análogos & derivados , Ciprofloxacino/administração & dosagem , Anti-Inflamatórios não Esteroides/administração & dosagem , Conjuntivite Viral/tratamento farmacológico , Diclofenaco/administração & dosagem , Corticosteroides/administração & dosagem , Soluções Oftálmicas/administração & dosagem , Prednisolona/administração & dosagem , Doença Aguda , Análise de Variância , Interleucinas/análise , Interferon gama , Fator de Necrose Tumoral alfa/análise , Resultado do Tratamento , Óxido Nítrico Sintase/análise , Lubrificantes Oftálmicos/administração & dosagem
6.
An. bras. dermatol ; 93(6): 829-835, Nov.-Dec. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-973620

RESUMO

Abstract: Background: Allergic contact dermatitis to ion nickel (Ni+2) is an inflammatory dermatosis, common in industrialized countries. It involves the activation of nickel-specific T-cells, followed by proliferation and induction of a mixed profile of both proinflammatory and regulatory cytokines, suggesting that several T-cell subtypes (helper - Th and cytotoxic - Tc) are involved. A broader understanding of the cytokine profile may lead to new therapeutic approaches. Objectives: This study aimed to analyze the cytokines TNF-α, INF-γ, IL-2, IL-4, IL-10, IL-13, IL-17 and IL-23 using the immunohistochemistry technique in order to try to identify their prevalence in chronic and acute eczema of patients with allergic contact dermatitis to Ni+2. Methods: We performed an immunohistochemical study for eight cytokines in 20 patients with Ni+2 allergic contact dermatitis, biopsied at the site of chronic eczema, triggered by the patient's daily contact with Ni+2, and at the site of acute eczema caused by nickel sulfate, 48 hours after applying the contact test. Results: The stained samples showed positive results for the eight cytokines studied. TNF-α, IFN-γ, IL-4, IL-13 and IL-17 had a higher prevalence in chronic eczema, IL-2 and IL-23 in acute eczema, and IL-10 presented a similar prevalence in both acute and chronic eczema. However, these prevalences were statistically significant only for IL-4 and IL-13. Study Limitations: Small sample size. Conclusions: In chronic and acute eczema, we observed the presence of a mixed cytokine profile of the T cell subtypes (Th/Tc), suggesting that the responses are expressed at the same time.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Citocinas/análise , Interleucinas/análise , Interferon gama/análise , Fator de Necrose Tumoral alfa/análise , Dermatite Alérgica de Contato/imunologia , Níquel/efeitos adversos , Biópsia , Imuno-Histoquímica , Doença Aguda , Doença Crônica , Estudos Prospectivos , Citocinas/imunologia , Interleucinas/imunologia , Interferon gama/imunologia , Fator de Necrose Tumoral alfa/imunologia , Dermatite Alérgica de Contato/etiologia , Dermatite Alérgica de Contato/patologia , Níquel/imunologia
7.
An. bras. dermatol ; 93(2): 302-303, Mar.-Apr. 2018. graf
Artigo em Inglês | LILACS | ID: biblio-1038265

RESUMO

Abstract: IL-22 has been implicated in the pathogenesis of vitiligo. However, the role of aryl hydrocarbon receptor transcription factor that acts as a master regulator of IL-22-producing Th22 cells is not fully understood. The goal of this study was to investigate the expression pattern of aryl hydrocarbon receptor in peripheral blood mononuclear cells of patients with vitiligo and in normal controls. Transcript levels were determined by a reverse transcription quantitative real-time polymerase chain reaction. Aryl hydrocarbon receptor mRNA expression was drastically increased in patients with vitiligo compared to healthy controls (P = 0.000). Th22 cells may contribute to abnormal immune responses underlying vitiligo.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Vitiligo/genética , Regulação para Cima/genética , Receptores de Hidrocarboneto Arílico/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , RNA Mensageiro/genética , Estudos de Casos e Controles , Expressão Gênica , Interleucinas/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa
8.
Electron. j. biotechnol ; 32: 63-68, Mar. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1022642

RESUMO

Background: Although immunosuppressive therapies have made organ transplantation a common medical procedure worldwide, chronic toxicity has a major issue for long-term treatment. One method to improve therapies and methods is the application of immunomodulatory agents from parasites such as Hypoderma lineatum. Hypodermin A (HA) is a serine esterase secreted by the larvae of Hypoderma lineatum, several studies demonstrated its immunosuppressive mechanism in vitro, and recently we discovered that HA inhibits the expression of interferon (IFN)-γ and interleukin (IL)-2 and activates IL-10 expression. Therefore, we hypothesized that it might be a potential agent used to block allograft rejections. However, most studies of the immunosuppressive mechanisms associated with HA were undertaken at the cellular level. In order to augment these studies, we evaluated the immunosuppressive effects of HA in vivo using an HA transgenic mouse model. Result: Our results revealed similar findings to those reported by in vitro studies, specifically that HA induced prostaglandin E2 expression, downregulated IFN-γ and IL-2 expression, and promoted IL-10 secretion via E-type prostanoid receptor 4. Additionally, we observed that HA overexpression inhibited lipopolysaccharide-induced TLR4 activation. These findings provide insight into a new potential agent capable of blocking graft rejection. Conclusion: Our founding suggested that HA-related treatment could be a promising option to improve the viability of grafts in human.


Assuntos
Animais , Camundongos , Serina Endopeptidases/imunologia , Dípteros/enzimologia , Dípteros/imunologia , Rejeição de Enxerto/imunologia , Ensaio de Imunoadsorção Enzimática , Serina Endopeptidases/metabolismo , Western Blotting , Citocinas , Imunossupressão , Interleucinas/antagonistas & inibidores , Interferons/antagonistas & inibidores , Interleucina-10/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptor 4 Toll-Like , Reação em Cadeia da Polimerase em Tempo Real , Rejeição de Enxerto/enzimologia , Rejeição de Enxerto/prevenção & controle
9.
An. bras. dermatol ; 93(1): 63-66, Jan.-Feb. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-887148

RESUMO

Abstract: Background: Psoriasis is a chronic inflammatory disorder, characterized by increased keratinocyte proliferation due to abnormal differentiation of basal keratinocytes. The etiology of the disease is unclear, and according to the survey results, it is hypothesized that a combination of genetic and environmental factors prompts an abnormal immune response in patients with psoriasis. CD4+ Th cells play a multifaceted role in both immune defense and pathogenesis of certain diseases such as psoriasis. Nonetheless, the exact contribution of different subpopulations of Th cells in psoriasis is still not clear. Objective: The aim of present study was to determine the mRNA expression level of RORC as potential inducer of Th17 cell differentiation and expression pattern of Th17-signature cytokines (IL-17A and IL-22). Methods: Twenty patients with psoriasis and twenty-one healthy subjects were included in the study. Peripheral blood mononuclear cells (PBMCs) were separated and expression of three genes were determined by quantitative real-time reverse transcriptase PCR (qRT-PCR). Plasma levels of IL-17 and IL-22 were also evaluated by ELISA. Results: RORC, IL-17A and IL-22 gene expression was significantly higher in patients with psoriasis compared with healthy controls (P<0.05). In addition, a marked increase in plasma IL-17A and IL-22 levels was observed in patient group compared to controls (P<0.001). Study limitations: small number of patients. Conclusion: These data suggest that Th17 response may contribute to the pathogenesis of psoriasis.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Psoríase/metabolismo , Queratinócitos/fisiologia , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/fisiologia , Células Th17/metabolismo , Psoríase/etiologia , Índice de Gravidade de Doença , RNA Mensageiro/metabolismo , Estudos de Casos e Controles , Expressão Gênica , Queratinócitos/citologia , Diferenciação Celular , Interleucinas/sangue , Interleucina-17/sangue , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , Células Th17/imunologia
10.
Rev. bras. psiquiatr ; 39(3): 195-200, July-Sept. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-899361

RESUMO

Objective: To investigate plasma concentrations of S100B (a calcium-binding protein derived primarily from the glia) and inflammatory cytokines in children with autism and the relationship between S100B and cytokine concentrations. Methods: Plasma levels of S100B, tumor necrosis factor alpha (TNF-α), interferon gamma, interleukin (IL)-1β, IL-4, IL-6, IL-10, and IL-17A were measured in 40 unmedicated children with autism and 35 normally developing healthy children. The severity of autism was assessed using the Childhood Autism Rating Scale (CARS). Results: Concentrations of both S100B and TNF-α were higher in children with autism before and after adjusting for a priori-selected confounders (age, sex, and body mass index). S100B concentrations were higher in children with severe autism compared to children with mild-moderate autism. However, this association remained as a trend after adjusting for confounders. S100B concentrations correlated positively with TNF-α concentrations. Conclusion: Our findings showing an increase in peripheral concentrations of S100B and TNF-α provide limited support to the hypothesis about the roles of altered immune function and S100B in autism spectrum disorder (ASD). Studies of larger numbers of well-characterized individuals with ASD are needed to clarify the potential role of the immune system in the pathophysiology of this disorder.


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Fator de Necrose Tumoral alfa/sangue , Subunidade beta da Proteína Ligante de Cálcio S100/sangue , Transtorno do Espectro Autista/sangue , Índice de Gravidade de Doença , Biomarcadores/sangue , Índice de Massa Corporal , Estudos Transversais , Interleucinas/sangue
11.
An. bras. dermatol ; 92(4): 534-536, July-Aug. 2017. graf
Artigo em Inglês | LILACS | ID: biblio-887014

RESUMO

Abstract: We report a 12-year-old girl who presented with recurrent angioedema on the face, trunk, and extremities, and concomitant marked weight gain for 5 years. During the episode, her white blood cell count increased to 47.7×109/L with 89.9% eosinophils, followed by elevated serum level of IL-5, IgE, IgM, and LDH. Histopathology showed perivascular eosinophilic infiltration and diffuse eosinophilic infiltration throughout the dermis. Possible causes of hypereosinophilia and eosinophilic infiltration of vital organs were ruled out. We also tested the FIP1L1/PDGFRa and ETV6/PDGFRb fusion gene to exclude the possibility of myeloid and lymphatic vessel neoplasms. The patient was treated with methylprednisolone and discharged with an oral prednisolone taper, which resulted in complete remission of the edema and normalization of peripheral blood eosinophil count, serum IL-5 level, IgE, IgM, and LDH.


Assuntos
Humanos , Feminino , Criança , Eosinofilia/complicações , Angioedema/complicações , Angioedema/patologia , Recidiva , Imunoglobulina E/sangue , Imunoglobulina M/sangue , Ganho de Peso , Interleucinas/sangue , Eosinofilia/patologia
12.
Braspen J ; 32(2): 155-159, abr.-jun. 2017.
Artigo em Português | LILACS | ID: biblio-848203

RESUMO

Objetivos: Determinar a presença de estresse oxidativo e inflamação no intestino de pacientes com doença celíaca. Método: Foi realizado estudo transversal que incluiu pacientes submetidos à endoscopia gastrointestinal. A população do estudo consistiu em 24 casos e 26 controles. Foram medidos os níveis duodenais de proteínas carboniladas, espécies reativas ao ácido tiobarbitúrico, bem como catalase (CAT), superóxido dismutase (SOD). Também foram determinados os níveis intestinais de interleucina (IL) 6, 10 e 8. A classificação de Marsh foi registrada e utilizada como parâmetro de gravidade da doença. Resultados: Tanto a IL-6 como a IL-10, mas não a IL8, aumentaram nos pacientes com doença celíaca quando comparados com indivíduos saudáveis. Os parâmetros de dano oxidativo foram aumentados,enquanto que as defesas antioxidantes foram reduzidas em nossa amostra. Os níveis de IL6 ea atividade do SOD foram relacionados com a pontuação de Marsh. Conclusões: Diferentes marcadores de inflamação e estresse oxidativo estão alterados no intestino de pacientes com doença celíaca, e alguns deles estão relacionados à gravidade da doença.(AU)


Objectives: Determine the presence of oxidative stress and inflammation in the gut of patients with celiac disease. Methods: Transversal study that included patients undergoing upper gastrointestinal endoscopy was performed. The study population consisted 24 cases and 26 controls. The duodenal levels of protein carbonyls, thiobarbituric acid reactive species, as well as catalase, superoxide dismutase (SOD) activities were measured. Gut levels of interleukin (IL) 6, 10 and 8 were also determined.The Marsh classification was recorded and used as a parameter of disease severity. Results: Both IL-6 and IL-10, but not IL8, were increased in celiac disease patients when compared to healthy individuals. Oxidative damage parameters were increased while antioxidant defenses were decreased in our sample. Both IL6 levels and SOD activity were related to Marsh score. Conclusions: Different markers of inflammation and oxidative stress are altered in the gut of celiac disease patients, and some of them are related to disease severity.(AU)


Assuntos
Humanos , Doenças Inflamatórias Intestinais , Doença Celíaca/patologia , Estresse Oxidativo , Superóxido Dismutase/análise , Catalase/análise , Estudos Transversais/instrumentação , Endoscopia Gastrointestinal/instrumentação , Interleucinas/análise , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Carbonilação Proteica
13.
Acta cir. bras ; 32(5): 376-387, May 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-837712

RESUMO

Abstract Purpose: To investigate whether modulating GSK-3β could attenuate myocardial ischemia reperfusion injury (MIRI) induced acute lung injury (ALI) and analyze the underlying mechanism. Methods: Male SD rats were subjected to MIRI with or without myocardial ischemic post-conditioning in the presence or absence of GSK-3β inhibitor. GSK-3β inhibitor was injected peritoneally 10min before MIRI. Lung W/D weight ratio, MPO, PMNs, histopathological changes, TUNEL, Bax, Bcl-2, IL-6, IL-8, IL-10, GSK-3β, and caspase-3 were evaluated in the lung tissues of all rats. Results: After MIRI, lung injury was significantly increased manifested as significant morphological changes and increased leukocytes in the interstitial capillaries, Lung W/D ratio, MPO, and PMN in BALF, which was associated with enhanced inflammation evidenced by increased expressions of IL-6, IL-8 and reduced expression of IL-10. MIRI significantly increased cell apoptosis in the lung as increased levels of apoptotosis, Bax, cleaved caspase-3, and reduced expression of Bcl-2 was observed, which was concomitant with reduced p-GSK-3β. All these changes were reversed/prevented by ischemic post-conditioning, while these beneficial effects of ischemic post-conditioning were abolished by GSK-3β inhibition. Conclusion: Myocardial ischemia reperfusion injury induces acute lung injury by induction of inflammation and cell apoptosis. Ischemic post-conditioning protects the lung from ALI following MIRI by increasing p-GSK-3β.


Assuntos
Animais , Masculino , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Substâncias Protetoras/metabolismo , Lesão Pulmonar Aguda/prevenção & controle , Pós-Condicionamento Isquêmico/métodos , Glicogênio Sintase Quinase 3 beta/metabolismo , Distribuição Aleatória , Regulação para Baixo , Interleucinas/metabolismo , Ratos Sprague-Dawley , Apoptose/efeitos dos fármacos , Peroxidase/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Substâncias Protetoras/farmacologia , Marcação In Situ das Extremidades Cortadas , Modelos Animais , Ativação Enzimática , Proteína X Associada a bcl-2/metabolismo , Caspase 3/metabolismo , Lesão Pulmonar Aguda/enzimologia , Glicogênio Sintase Quinase 3 beta/antagonistas & inibidores , Glicogênio Sintase Quinase 3 beta/farmacologia , Inflamação/metabolismo , Infarto do Miocárdio/patologia , Neutrófilos/enzimologia
14.
Appl. cancer res ; 37: 1-6, 2017. tab, ilus
Artigo em Inglês | LILACS, Inca | ID: biblio-915122

RESUMO

Background: Myelodysplastic syndromes (MDS) are a heterogeneous group of disorders characterized by ineffective hematopoiesis and risk of leukemia transformation. There is evidence to suggest the participation of immune system deregulation in MDS pathogenesis. Interleukin-32 (IL-32) is a newly described multifunctional cytokine reported as an important mediator in autoimmune and inflammatory disorders. In the present study, we reported the expression of IL32 and IL32 transcript variants (α, ß, γ and δ) in peripheral blood CD3+ cells from healthy controls and MDS patients. Methods: CD3+ cells were isolated by immunomagnetic cell sorting from thirty-nine untreated MDS patients and twenty-nine healthy donors. Gene expression was evaluated by quantitative PCR. For statistical analysis, Mann­Whitney test, Kruskal-Wallis test with Dunns post test and Log-rank (Mantel-Cox) were used, as appropriate. A p value <0.05 was considered statistically significant. Results: IL32 expression and IL32 transcript variants IL32α, IL32ß, IL32γ, and IL32δ, were similar in peripheral blood CD3+ cells from healthy donors and MDS patients. Increased IL-32α expression was an independent predictor for MDS disease progression by univariate and multivariate analysis. Conclusions: We observed that IL32 expression is not differently expressed in CD3+ cells from MDS patients; nevertheless IL32α has a potential role in disease progression (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Síndromes Mielodisplásicas , Análise Multivariada , Interleucinas , Complexo CD3 , Progressão da Doença , Sistema Imunitário
15.
Braz. oral res. (Online) ; 31: e8, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839531

RESUMO

Abstract The aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (ESSDAI) and subjective (ESSPRI) indexes in patients with primary Sjögren’s Syndrome (pSS). Twenty-eight female patients were divided into four groups: pSS patients with or without chronic periodontitis (SCP, SC, respectively), and systemically healthy patients with or without chronic periodontitis (CP, C, respectively). Periodontal clinical examination and immunological and microbiological sample collection were performed at baseline, 30 and 90 days after nonsurgical periodontal treatment (NSPT). Levels of interleukin IL-1β, IL-8 and IL-10 in saliva and gingival crevicular fluid (GCF) were evaluated by ELISA, as well as the expression of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans, (Aa) Tannerella forsythia (Tf), and Treponema denticola (Td), by qPCR. Systemic activity and pSS symptoms were evaluated by ESSDAI and ESSPRI. NSPT resulted in improved periodontal clinical parameters in both SCP and CP groups (p>0.05). Pg, Aa, and Tf levels decreased after NSPT only in CP patients (p<0.05). Significantly greater levels of IL-10 in GCF were verified in both SCP and CP groups (p<0.05). SCP patients showed increased salivary flow rates and decreased ESSPRI scores after NSPT. In conclusion, NSPT in pSS patients resulted in improved clinical and immunological parameters, with no significant effects on microbiological status. pSS patients also showed increased salivary flow and lower ESSPRI scores after therapy. Therefore, it can be suggested that NSPT may improve the quality of life of pSS patients.


Assuntos
Humanos , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Síndrome de Sjogren/complicações , Periodontite Crônica/etiologia , Periodontite Crônica/terapia , Saliva/química , Salivação/fisiologia , Taxa Secretória , Fatores de Tempo , Ensaio de Imunoadsorção Enzimática , Síndrome de Sjogren/fisiopatologia , Estudos de Casos e Controles , Reação em Cadeia da Polimerase , Estudos Prospectivos , Estudos Longitudinais , Líquido do Sulco Gengival , Interleucinas/análise , Resultado do Tratamento , Periodontite Crônica/fisiopatologia , Periodontite Crônica/microbiologia , Carga Bacteriana
16.
São Paulo; s.n; 2017. 111 p. ilus, tab.
Tese em Português | LILACS, Sec. Est. Saúde SP | ID: biblio-1085859

RESUMO

Introdução: Nas últimas décadas, a reestenose tem sido o "calcanhar de Aquiles" da intervenção coronária percutânea (ICP), limitando seus resultados no médio e longo prazo. Estudos preliminares demonstraram que após a injúria inicial com catéter balão e/ou com stents coronarianos, há desnudação endotelial, dissecção, deposição plaquetária e atração de leucócitos como resposta imediata, podendo levar à reestenose no seguimento mais tardio. A injúria local na parede arterial após implante de stent pode promover a expressão genética e liberação de mediadores inflamatórios, interleucinas, proteínas de fase aguda e fatores de coagulação, deposição de plaquetas e formação de trombos. Estes processos podem estar diretamente relacionados ao prognóstico da doença cardiovascular, porém são escassos os estudos que caracterizem a resposta inflamatória aguda pós-implante de stent coronariano e à ocorrência de eventos adversos. Objetivos: Neste estudo, pretendeu-se avaliar os efeitos de dose de ataque de Rosuvastatina (40mg) sobre a resposta inflamatória aguda após implante de stent coronariano, bem como correlacionar as variações das concentrações de citocinas e a respectiva expressão gênica. Métodos: Pacientes portadores de doença coronária estável sem uso de estatina (há mais de 7 dias), submetidos à intervenção coronária percutânea eletiva em artéria coronária nativa foram randomizados para receberem dose única de ataque de rosuvastatina (40 mg via oral, 3 horas prévias ao procedimento; Grupo Tratado (GT); n=63) versus Grupo Controle (GC) (Ausência da administração de rosuvastatina); n=61...


Assuntos
Expressão Gênica , Interleucinas , Mediadores da Inflamação , Rosuvastatina Cálcica , Stents
18.
Rev. bras. reumatol ; 56(5): 451-457, Sept.-Oct. 2016. tab
Artigo em Inglês | LILACS | ID: lil-798098

RESUMO

ABSTRACT A better understanding of the inflammatory mechanisms of rheumatoid arthritis and the development of biological therapy revolutionized its treatment, enabling an interference in the synovitis – structural damage – functional disability cycle. Interleukin 33 was recently described as a new member of the interleukin-1 family, whose common feature is its pro-inflammatory activity. Its involvement in the pathogenesis of a variety of diseases, including autoimmune diseases, raises the interest in the possible relationship with rheumatoid arthritis. Its action has been evaluated in experimental models of arthritis as well as in serum, synovial fluid and membrane of patients with rheumatoid arthritis. It has been shown that the administration of interleukin-33 exacerbates collagen-induced arthritis in experimental models, and a positive correlation between cytokine concentrations in serum and synovial fluid of patients with rheumatoid arthritis and disease activity was found. This review discusses evidence for the role of interleukin-33 with a focus on rheumatoid arthritis.


RESUMO A melhor compreensão dos mecanismos inflamatórios da artrite reumatoide e o desenvolvimento da terapia biológica revolucionaram o tratamento da doença, permitindo uma interferência no ciclo sinovite–dano estrutural–incapacidade funcional. A interleucina 33 foi recentemente descrita como um novo membro da família da interleucina 1, cuja característica comum é a atividade pró-inflamatória. Por estar envolvida na patogênese de uma grande variedade de doenças, incluindo doenças autoimunes, a interleucina 33 começa a ser estudada na doença reumatoide. Ela tem sido avaliada em modelos experimentais de artrite, no soro, no líquido e membrana sinoviais de pacientes com artrite reumatoide. Demonstrou-se que a administração da interleucina 33 exacerba a artrite induzida por colágeno em modelos experimentais, e concentrações dessa citocina no soro e no líquido sinovial de pacientes com artrite reumatoide correlacionaram-se positivamente com a atividade da doença. Esse manuscrito apresenta a interleucina 33 e discute as evidências do seu papel em diferentes doenças, com ênfase na artrite reumatoide.


Assuntos
Humanos , Animais , Artrite Experimental/imunologia , Artrite Reumatoide/patologia , Interleucina-33/imunologia , Interleucina-33/sangue , Artrite Experimental/patologia , Artrite Experimental/sangue , Artrite Reumatoide/imunologia , Artrite Reumatoide/sangue , Líquido Sinovial , Sinovite , Interleucinas
19.
Einstein (Säo Paulo) ; 14(3): 391-397, July-Sept. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-796967

RESUMO

ABSTRACT Objective: To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods. Methods: Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth factor-beta1 (TGF-β1). Results: The diverse separation methods generated systematically different profiles regarding number of platelets and leukocytes. The Medtronic system yielded a product with the highest concentration of platelets, and the open method, with the lowest concentration of platelets. The results of cytokine analysis showed that the different types of centrifugation yielded products with high concentrations of interleukin 8, interleukin 1β. The open system resulted in a product with high levels of interleukin 6. Other cytokines and chemokines measured were similar between systems. The product obtained with the open method showed higher levels of TGF-β1 in relation to other systems and low FGF-2 levels. Conclusion: The formed elements, growth factors and cytokines in samples of platelet-rich plasma varied according to the centrifugation technique used.


RESUMO Objetivo: Avaliar fatores de crescimento e citocinas em amostras de plasma rico em plaquetas obtidas por três diferentes métodos de centrifugação. Métodos: Foi coletado sangue periférico de seis indivíduos, sem doença hematológica, com idades entre 18 e 68 anos, para obtenção de plasma rico em plaquetas, utilizando o método aberto e sistemas comerciais das empresas Medtronic e Biomet. Os produtos obtidos com os diferentes tipos de centrifugação foram submetidos às análises laboratoriais, incluindo citocinas próinflamatórias e quimiocinas, por meio de ensaios de citometria de fluxo, concentração do fator de crescimento fibroblástico-2 (FGF-2) e fator de crescimento transformador-beta1 (TGF-β1). Resultados: As diferentes centrifugações geraram perfis sistematicamente diferentes referentes ao número de plaquetas e de leucócitos. O sistema da Medtronic originou produto com a maior concentração de plaquetas, e o método aberto com a menor concentração de plaquetas. Os resultados da análise de citocinas demonstraram que os diferentes tipos de centrifugação originaram produtos com elevadas concentrações de interleucina 8 e interleucina 1β. O sistema aberto resultou em produto com elevados níveis de interleucina 6. As demais citocinas e quimiocinas mensuradas foram similares entre os sistemas. O produto obtido com o método aberto apresentou níveis superiores de TGF-β1 em relação aos demais sistemas e reduzidos níveis de FGF-2. Conclusão: Os elementos figurados, fatores de crescimento e citocinas, em amostras de plasma rico em plaquetas, variaram conforme a técnica de centrifugação utilizada.


Assuntos
Humanos , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Citocinas/análise , Peptídeos e Proteínas de Sinalização Intercelular/análise , Plasma Rico em Plaquetas/química , Centrifugação/métodos , Citocinas/sangue , Interleucinas/análise , Interleucinas/sangue , Quimiocinas/análise , Quimiocinas/sangue , Peptídeos e Proteínas de Sinalização Intercelular/sangue , Lesões do Manguito Rotador/cirurgia
20.
J. appl. oral sci ; 24(4): 352-358, July-Aug. 2016. tab
Artigo em Inglês | LILACS | ID: lil-792599

RESUMO

ABSTRACT Periodontal disease has been associated with elevations of blood cytokines involved in atherosclerosis in systemically healthy individuals, but little is known about this association in stable cardiovascular patients. The aim of this study was to assess the association between periodontal disease (exposure) and blood cytokine levels (outcomes) in a target population of patients with stable coronary artery disease (CAD). Material and Methods This cross-sectional study included 91 patients with stable CAD who had been under optimized cardiovascular care. Blood levels of IL-1β, IL-6, IL-8, IL-10, IFN-γ, and TNF-α were measured by Luminex technology. A full-mouth periodontal examination was conducted to record probing depth (PD) and clinical attachment (CA) loss. Multiple linear regression models, adjusting for gender, body mass index, oral hypoglycemic drugs, smoking, and occurre:nce of acute myocardial infarction were applied. Results CAD patients that experienced major events had higher concentrations of IFN-γ (median: 5.05 pg/mL vs. 3.01 pg/mL; p=0.01), IL-10 (median: 2.33 pg/mL vs. 1.01 pg/mL; p=0.03), and TNF-α (median: 9.17 pg/mL vs. 7.47 pg/mL; p=0.02). Higher numbers of teeth with at least 6 mm of CA loss (R2=0.07) and PD (R2=0.06) were significantly associated with higher IFN-γ log concentrations. Mean CA loss (R2=0.05) and PD (R2=0.06) were significantly related to IL-10 concentrations. Elevated concentrations of TNF-α were associated with higher mean CA loss (R2=0.07). Conclusion Periodontal disease is associated with increased systemic inflammation in stable cardiovascular patients. These findings provide additional evidence supporting the idea that periodontal disease can be a prognostic factor in cardiovascular patients.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Doenças Periodontais/sangue , Doença da Artéria Coronariana/sangue , Interleucinas/sangue , Interferon gama/sangue , Fator de Necrose Tumoral alfa/sangue , Doenças Periodontais/fisiopatologia , Valores de Referência , Doença da Artéria Coronariana/fisiopatologia , Biomarcadores/sangue , Fumar/efeitos adversos , Modelos Lineares , Índice Periodontal , Estudos Transversais , Valor Preditivo dos Testes , Inquéritos e Questionários , Fatores de Risco , Perda da Inserção Periodontal
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