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An. bras. dermatol ; 94(5): 542-548, Sept.-Oct. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1054874


Abstract Background There is an obvious need for more prompt and specific biomarkers of bacterial infections in generalized pustular psoriasis patients. Objective The aim of this study was to evaluate the diagnostic properties and define appropriate cut-off values of procalcitonin and C-reactive protein in predicting bacterial infection in generalized pustular psoriasis patients. Methods Sixty-four generalized pustular psoriasis patients hospitalized from June 2014 to May 2017 were included in this retrospective study. The values of procalcitonin, C-reactive protein, details of infection, and other clinical parameters were analyzed. Results Receiver operating characteristic curve analysis generated similar areas (p = 0.051) under the curve for procalcitonin 0.896 (95% CI 0.782-1.000) and C-reactive protein 0.748 (95% CI 0.613-0.883). A cut-off value of 1.50 ng/mL for procalcitonin and 46.75 mg/dL for C-reactive protein gave the best combination of sensitivity (75.0% for procalcitonin, 91.7% for C-reactive protein) and specificity (100% for procalcitonin, 53.8% for C-reactive protein). Procalcitonin was significantly positively correlated with C-reactive protein levels both in the infected (r = 0.843, p = 0.040) and non-infected group (r = 0.799, p = 0.000). Study limitations The sample size and the retrospective design are limitations. Conclusions The serum levels of procalcitonin and C-reactive protein performed equally well to differentiate bacterial infection from non-infection in generalized pustular psoriasis patients. The reference value of procalcitonin and C-reactive protein applied to predicting bacterial infection in most clinical cases may not be suitable for generalized pustular psoriasis patients. C-reactive protein had better diagnostic sensitivity than procalcitonin; however, the specificity of procalcitonin was superior to that of C-reactive protein.

Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Psoríase/microbiologia , Psoríase/sangue , Infecções Bacterianas/sangue , Proteína C-Reativa/análise , Pró-Calcitonina/sangue , Valores de Referência , Temperatura Corporal , Biomarcadores/sangue , Reprodutibilidade dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , Estatísticas não Paramétricas , Contagem de Leucócitos , Neutrófilos
An. bras. dermatol ; 94(5): 549-552, Sept.-Oct. 2019. tab
Artigo em Inglês | LILACS | ID: biblio-1054854


Abstract Background Nipple eczema is a less common presentation of atopic dermatitis. No studies in the literature have correlated nipple eczema in pregnancy as a manifestation of atopic dermatitis. Objective To evaluate whether nipple eczema presenting in pregnancy is a manifestation of atopic dermatitis. Methods This was a prospective observational study including 100 women who presented with nipple eczema for the first time during pregnancy. The exclusion criteria were any patient with previous history of nipple eczema, those already on oral or topical treatment for atopic dermatitis or nipple eczema, and other disorders mimicking eczema. Patients were divided into two groups ‒ nipple eczema with atopic dermatitis and without atopic dermatitis. Demographic data, clinical features, total leukocyte count, differential leukocyte count, absolute eosinophil counts, and serum IgE levels were compared between the two groups to detect association between nipple eczema in pregnancy and atopic dermatitis. Results Out of 100 patients, 39 were diagnosed with atopic dermatitis, whereas 61 were ruled out to have any features suggestive of atopic dermatitis. There were no statistically significant differences in mean age, mean duration of symptoms, and serum IgE levels. In patients with atopic dermatitis, bilateral symptoms were noted more commonly than in patients without the disease, but this was statistically insignificant. Study limitations Lack of long term follow-up and no large studies in literature to compare results. Conclusion Nipple eczema in pregnancy follows a similar pattern as in other age groups. The clinical profile of patients is similar in cases with and without atopic dermatitis.

Humanos , Feminino , Gravidez , Adulto , Doenças Mamárias/patologia , Dermatite Atópica/patologia , Eczema/patologia , Mamilos/patologia , Complicações na Gravidez/patologia , Doenças Mamárias/diagnóstico , Doenças Mamárias/sangue , Imunoglobulina E/sangue , Estudos Prospectivos , Dermatite Atópica/diagnóstico , Dermatite Atópica/sangue , Eczema/diagnóstico , Eczema/sangue , Índia , Contagem de Leucócitos , Neutrófilos
Rev. Paul. Pediatr. (Ed. Port., Online) ; 37(3): 318-324, July-Sept. 2019. tab
Artigo em Inglês | LILACS | ID: biblio-1041334


ABSTRACT Objective: To investigate the influence of patient age on the diagnosis and management of appendicitis, as well as to evaluate the rate of complications according to the age group. Methods: We undertook a retrospective analysis of 1,736 children who underwent laparoscopic appendectomy in our center between January 2000 and December 2013. Patients were divided in groups taken into account their age: group A were infants, group B were preschoolers, group C were those ones older than five years old, and group D were those ones younger than five years old. A p value of 0.05 was considered statistically significant. Results: We found higher incidence of misdiagnosis and atypical symptoms in the youngest patients. The rate of perforation was similar between group A and B (p=0.17). However, it was higher in group D than in group C (p<0.0001). The incidence of postoperative complications was higher in the youngest patients too (p=0.0002). Conclusions: The age does make a difference in acute appendicitis. Because of its unusual presentation in children younger than five years old, it is often misdiagnosed, which leads to an increased morbidity. Although clinical presentation varies between infants and preschoolers, no statistically significant differences were observed in the rate of perforated appendix or postoperative complications.

RESUMO Objetivo: Investigar a influência da idade do paciente no diagnóstico e tratamento de apendicite, bem como avaliar a frequência de complicações dependendo da faixa etária. Métodos: Análise retrospectiva dos 1.736 pacientes pediátricos que foram submetidos à apendicectomia laparoscópica em nosso hospital de janeiro de 2000 a dezembro de 2013. Os pacientes foram divididos em grupos de acordo com sua idade: grupo A eram crianças, grupo B eram pré-escolares, grupo C eram maiores de cinco anos de idade e grupo D eram menores de cinco anos de idade. Considerou-se estatisticamente significante p-valor <0,05. Resultados: Encontramos maior incidência de diagnóstico incorreto e sintomas atípicos em pacientes mais novos. A taxa de perfuração foi semelhante entre os grupos A e B (p=0.17); foi maior, porém, no grupo D que no grupo C (p<0.0001). A incidência de complicações no pós-operatório também foi maior em pacientes mais novos (p=0.0002). Conclusões: A idade faz diferença em casos de apendicite aguda. Por causa da sua apresentação rara em crianças menores de cinco anos, é frequentemente diagnosticada incorretamente, o que aumenta a morbidade. Apesar de sua apresentação clínica variar entre lactentes e pré-escolares, não foram observadas diferenças estatisticamente significativas na proporção de apêndices perfurados nem na de complicações pós-operatórias.

Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Pré-Escolar , Criança , Adolescente , Apendicectomia/estatística & dados numéricos , Apendicite/diagnóstico , Dor Abdominal/diagnóstico , Apendicite/cirurgia , Biomarcadores/sangue , Dor Abdominal/cirurgia , Estudos Retrospectivos , Erros de Diagnóstico/estatística & dados numéricos , Contagem de Leucócitos
Prensa méd. argent ; 105(5): 164-171, jun 2019. tab, fig
Artigo em Inglês | LILACS, BINACIS | ID: biblio-1024547


Background: Anemia is considered by Worl Health Organization (WHO) as a global health problem. It can be affected by the body mass index of an individual since increasing weight can result in low-grade systemic inflammation and elevation of hepcidin which results in seguestratiion of iron inside a variety of cells including macrophages, hepatocytes and enteroytes and this would lead to the development of what is known as anemia of inflammation. In this study, we aimed to evaluate the effects of BI on complete blood count parameters. Patients and methods: In this crosssectional, retrospective study: the data of 200 overweight and obese male patients aged (18-60 years) were collected. They were grouped according to BMI into everweight and obese: different CBC parameters were noted. Inter group comparison was applied regarding different blood parameters and the relations eith BMI were calculated. Results: The prevalence of anemia was 16%. An extremely significant difference was found in mean white blood cells count in relation to different BMI gropups (p=0.001). A positive linear correlation was found between BMI with RDW, WBS, and platelets. Conclusions: There were effects of increasing BMI on WBC, RDW and platelets (AU)

Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Alterações do Peso Corporal , Índice de Massa Corporal , Coleta de Dados/estatística & dados numéricos , Anemia/diagnóstico , Anemia/patologia , Anemia/sangue , Contagem de Leucócitos
Rev. bras. ter. intensiva ; 31(1): 64-70, jan.-mar. 2019. tab
Artigo em Português | LILACS | ID: biblio-1003617


RESUMO Objetivo: Avaliar a razão neutrófilo-linfócito na predição de sepse e mortalidade em pacientes admitidos em uma unidade de terapia intensiva. Métodos: Estudo de caso-controle de pacientes adultos admitidos em terapia intensiva. Foram incluídos como casos pacientes que tiveram sepse como razão de admissão e possuíam exame laboratorial de hemograma prévio. As análises estatísticas realizadas foram curva ROC, regressão logística binária, Mann Whitney e qui-quadrado de Pearson. Foi considerado significativo valor de p < 0,05. Resultados: Os valores de curva ROC foram 0,62 para razão neutrófilo-linfócito, 0,98 para neutrófilos bastonados e 0,51 para leucócitos totais. A presença de razão neutrófilo-linfócito superior a 5,0, o número de leucócitos acima de 12.000mm3/mL e número de neutrófilos bastonados acima 10% foram fatores de risco para sepse, entretanto somente os escores SAPS 3 e SOFA estavam relacionados a mortalidade dos pacientes. Conclusão: A razão neutrófilo-linfócito e os neutrófilos bastonados em combinação com outros parâmetros podem ser marcadores na detecção precoce de sepse em terapia intensiva.

ABSTRACT Objective: To evaluate the neutrophil-lymphocyte ratio as a predictor of sepsis and mortality in patients admitted to an intensive care unit. Methods: Case-control study of adult patients admitted to an intensive care unit. Patients who had sepsis as the reason for admission and who had a previous complete blood count examination were included as case patients. The following statistical analyses were performed: ROC curves, binary logistic regression, and Mann-Whitney and Pearson's chi-square tests. p < 0.05 was considered significant. Results: The ROC curve values were 0.62 for neutrophil-lymphocyte ratio, 0.98 for band neutrophils and 0.51 for total leukocytes. The presence of a neutrophil-lymphocyte ratio greater than 5.0, leukocyte count above 12,000mm3/mL and band neutrophil percentage above 10% were risk factors for sepsis; however, only the SAPS 3 and SOFA score were related to patient mortality. Conclusion: The neutrophil-lymphocyte ratio and band neutrophils in combination with other parameters may be markers for the early detection of sepsis in intensive care units.

Humanos , Masculino , Feminino , Idoso , Linfócitos/metabolismo , Sepse/diagnóstico , Unidades de Terapia Intensiva , Neutrófilos/metabolismo , Biomarcadores/sangue , Estudos de Casos e Controles , Modelos Logísticos , Fatores de Risco , Sepse/sangue , Diagnóstico Precoce , Contagem de Leucócitos , Pessoa de Meia-Idade
J. pediatr. (Rio J.) ; 94(5): 546-553, Sept.-Oct. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-975984


Abstract Objective: There is evidence of an important role of immune system changes in the triggering and maintenance of idiopathic nephrotic syndrome (INS). The aim of this study was to investigate the expression of cytokines in lymphocyte populations of patients with INS in comparison to healthy individuals, according to proteinuria. Methods: This cross-sectional study included 44 patients with INS and eight healthy children, matched for age and sex (controls). Patients were subdivided according to proteinuria: persistent proteinuria or partial remission (PP ≥ 300 mg/24 h, n = 17) and low proteinuria or complete remission (LP < 300 mg/24 h, n = 27). Ex vivo analysis of peripheral blood leukocytes by flow cytometry was performed using surface markers for T-lymphocytes, TCD4, TCD8, natural killer (NK) cells, NKT, and B-lymphocytes. Frequencies of intracellular cytokines were analyzed in these cells. Results: The frequencies of B-lymphocytes, NK cells, and NKT cells were lower in INS than in controls, whereas INS patients had a higher frequency of CD4+tumor necrosis factor (TNF)-α+ cells than controls. Cytotoxic-T-lymphocytes expressing IFN-γ were lower in INS than in controls. Patients with PP showed higher frequencies of CD4-T-lymphocytes expressing IFN-γ and TNF-α than controls. CD8-lymphocytes expressing TNF-α were increased in PP group when compared with LP and controls, while CD8+interferon (IFN)-γ+ cells were lower than in LP and in controls. Conclusion: Regardless the level of proteinuria, INS patients had increased expression of TNF-α in CD4-lymphocytes and reduced expression of IFN-γ in CD8-lymphocytes. Persistence of proteinuria was associated with higher levels of inflammatory markers.

Resumo Objetivo Há comprovação do importante papel das alterações no sistema imunológico no desencadeamento e manutenção da síndrome nefrótica idiopática (SNI). O objetivo deste estudo foi investigar a expressão das citocinas em populações de linfócitos de pacientes com SNI em comparação a indivíduos saudáveis e de acordo com a proteinúria. Métodos Este estudo transversal incluiu 44 pacientes com SNI e oito crianças saudáveis, pareados por idade e sexo (controles). Os pacientes foram subdivididos de acordo com a proteinúria: proteinúria persistente ou remissão parcial (PP ≥ 300 mg/24 h, n = 17) e proteinúria baixa ou remissão completa (PB < 300 mg/24 h, n = 27). A análise ex vivo de leucócitos no sangue periférico por citometria de fluxo foi feita utilizando marcadores de superfície para linfócitos T, TCD4, TCD8, células natural killer (NK), linfócitos NKT e B. As frequências das citocinas intracelulares foram analisadas nessas células. Resultados A frequência dos linfócitos B, células NK e células NKT foi menor em pacientes com SNI do que nos controles, ao passo que os pacientes com SNI apresentaram maior frequência de células CD4+fator de necrose tumoral (TNF)-α+ do que nos controles. Os linfócitos T citotóxicos que expressam interferon (IFN)-γ foram menores nos pacientes com SNI do que nos controles. Os pacientes com PP mostraram maiores frequências de linfócitos T CD4 que expressam IFN-γ e TNF-α que os controles. Os linfócitos CD8 que expressam TNF-α apresentaram aumento no grupo com PP, em comparação aos com PB e os controles, apesar de as células CD8+IFN-γ+ serem mais baixas nos pacientes com PB e nos controles. Conclusão Com relação ao nível de proteinúria, os pacientes com SNI apresentaram aumento na expressão de TNF-α nos linfócitos CD4 e expressão reduzida de IFN-γ nos linfócitos CD8. A persistência da proteinúria foi associada a maiores níveis de marcadores inflamatórios.

Humanos , Masculino , Feminino , Criança , Adolescente , Proteinúria/etiologia , Células Matadoras Naturais/imunologia , Linfócitos T/imunologia , Citocinas/imunologia , Síndrome Nefrótica/imunologia , Proteinúria/imunologia , Proteinúria/sangue , Biomarcadores , Estudos de Casos e Controles , Estudos Transversais , Citocinas/sangue , Progressão da Doença , Citometria de Fluxo , Contagem de Leucócitos , Síndrome Nefrótica/complicações , Síndrome Nefrótica/sangue
Int. j. cardiovasc. sci. (Impr.) ; 31(5)set.-out. 2018. ilus
Artigo em Inglês | LILACS | ID: biblio-914777


The development of cardiovascular diseases with atherosclerotic origin is associated with a severe inflammatory process. Neutrophils and lymphocytes are cells sensitive to this type of disorder and their ratio, known as the NLR (neutrophil/lymphocyte ratio), has shown to be useful in clinical practice. The aim of this study was to assess the role of NLR in cardiovascular disease risk assessment. We carried out a literature review in the PubMed databases searching for articles published between 2001 to 2017 and found that NLR is in fact a useful marker for cardiovascular disease. Using NLR in patients at cardiovascular risk would be useful to delineate the prognosis of patients with this disease pattern

Humanos , Masculino , Feminino , Doenças Cardiovasculares/mortalidade , Doenças Cardiovasculares/fisiopatologia , Contagem de Leucócitos , Neutrófilos , Aterosclerose/fisiopatologia , Biomarcadores , Angiografia Coronária/métodos , Vasos Coronários , Inflamação , Infarto do Miocárdio/mortalidade , Placa Aterosclerótica/complicações , Placa Aterosclerótica/diagnóstico , Prognóstico , Fatores de Risco
Rev. méd. hered ; 29(2): 97-101, abr. 2018. graf
Artigo em Espanhol | LILACS, LIPECS | ID: biblio-1014299


La alfalfa, Medicago sativa L, es una leguminosa altamente ecológica, de fácil cultivo y comercialización. En Perú, se cultiva como forraje para animales. Objetivos: Determinar el efecto del consumo del extracto de alfalfa sobre el recuento de leucocitos, en ratón albino. Material y métodos: Estudio experimental, realizado en el Bioterio de la Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos. Se utilizaron veinte ratones albinos de la cepa balb/c, machos, de peso promedio 23,2 ± 32,8 g. Se formaron dos grupos: experimental y control, cada uno, de diez ratones. Al grupo experimental se le administró, vía oral por canulación extracto de alfalfa, en dosis de 250 mg/kg, volumen de 2 ml, y al grupo control, también, vía oral por canulación, placebo, volumen de 2 ml, cada cinco horas, durante una semana, alternando con nutrientes estándar. Se realizó recuento de leucocitos. Resultados: Dentro del periodo de acondicionamiento, se observó un ligero incremento en la media de leucocitos del grupo control (3940 ± 1549,3) frente al grupo experimental (3840 ± 1468,3), cuya diferencia no fue significativa. Luego de administrar al grupo experimental el extracto de Medicago sativa L., alternando con sus nutrientes respectivos, se observó diferencia significativa de la media (7800 ± 2885,2) frente al grupo control (3930± 1518,1) (p<0,05). Conclusiones: En condiciones experimentales, el consumo del extracto de Medicago sativa L. incrementó significativamente el número de leucocitos. (AU)

Alfalfa, Medicago sativa L, is a highly organic legume, easy to grow and commercialize. In Peru, it is cultivated as fodder for animals. Objectives: To determine the effect of alfalfa extract consumption on the white blood cell count in albino mouse. Methods: Experimental study. carried out in Bioterio of the Facultad de Farmacia y Bioquímica of Universidad Nacional Mayor de San Marcos. Twenty albino mice of the balb / c strain, male, of average weight 23.2 ± 32.8 g were used. Two groups were formed: experimental and control, each of ten mice. The experimental group was administered, orally by cannulation alfalfa extract, in a dose of 250 mg / kg, volume of 2 ml, and the control group, also, orally by cannulation, placebo, volume of 2 ml, every five hours, for a week, alternating with standard nutrients. Leukocyte count was performed. Results: Within the conditioning period, there was a slight increase in leukocytes mean of the control group (3940 ± 1549.3) compared to the experimental group (3840 ± 1468.3), whose difference was not significant. After administering the extract of Medicago sativa L to the experimental group, alternating with their respective nutrients, a significant difference was observed between the mean (7800 ± 2885.2) and the control group (3930 ± 1518.1) (p <0.05). Conclusions: Under experimental conditions, the consumption of Medicago sativa extract significantly increased the number of leukocytes. (AU)

Animais , Camundongos , Consumo de Alimentos , Medicago sativa , Contagem de Leucócitos , Camundongos
Biosci. j. (Online) ; 34(2): 410-422, mar./apr. 2018. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-966651


The objective of this study was to measure the effects of glucose and salt level on white blood cells, red blood cells and platelets (PLTs) in the blood of a leukemic patient by using a white light microscope. Different concentrations of glucose and salt in the range of 0 mM to 500 mM were admixed in the blood sample to prepare blood smear. We revealed that shape of erythrocytes, leukocytes and platelets changes and form aggregates. Increasing concentrations of glucose cause to increases aggregation process of white blood cells, red blood cells and platelets. And the increasing concentration of sodium chloride causes to increase rouleaux formation and aggregation of platelets but dehydration due to increased sodium chloride concentration causes to break the aggregation of white blood cells. Comparison of CBC reports of these samples with and without analytes shows that total leukocyte count (TLC) decreases gradually towards normal ranges of leukocytes which is favorable in the treatment of leukemia but at the same time decreasing level of hemoglobin HGB, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and increasing level of red blood cell (RBCs) causes to reduce oxygen supply which is in favor of cancer growth and anemia. This work provides us the base for translation this in vitro study towards the in vivo case of blood microvasculature as a non-invasive methodology.

O objetivo deste estudo foi medir os efeitos da glicose e do nível de sal nos glóbulos brancos, glóbulos vermelhos e plaquetas (PLTs) no sangue de um paciente leucêmico usando um microscópio de luz branca. Foram misturadas diferentes concentrações de glicose e sal na gama de 0 mM a 500 mM na amostra de sangue para preparar esfregaço de sangue. Descrevemos que a forma dos eritrócitos, leucócitos e plaquetas muda e forma agregados. O aumento das concentrações de glicose aumenta o processo de agregação de glóbulos brancos, glóbulos vermelhos e plaquetas. E a crescente concentração de cloreto de sódio causa o aumento da formação de rouleaux e a agregação de plaquetas, mas a desidratação devido ao aumento da concentração de cloreto de sódio causa a quebra da agregação de glóbulos brancos. A comparação dos relatórios de CBC dessas amostras com e sem analitos mostra que a contagem total de leucócitos (TLC) diminui gradualmente para os intervalos normais de leucócitos, o que é favorável no tratamento da leucemia, mas ao mesmo tempo diminui o nível de hemoglobina HGB, hemoglobina corpuscular média (MCH ), a concentração média de hemoglobina corpuscular (MCHC) e o aumento do nível de glóbulos vermelhos (RBCs) reduz o suprimento de oxigênio, o que é a favor do crescimento do câncer e da anemia. Este trabalho fornece a base para a tradução deste estudo in vitro para o caso in vivo de microvasculatura de sangue como uma metodologia não-invasiva.

Leucemia , Eritrócitos , Leucócitos , Microscopia , Contagem de Células Sanguíneas , Glicemia , Cloreto de Sódio , Índices de Eritrócitos , Contagem de Leucócitos
Arch. argent. pediatr ; 116(1): 1-7, feb. 2018. graf, tab
Artigo em Inglês, Espanhol | LILACS, BINACIS | ID: biblio-887426


Antecedentes: Con el incremento de la proctocolitis alérgica inducida por proteínas de la dieta (PAIPD), son necesarios estudios que aclaren su fisiopatología y determinar marcadores no invasivos y sencillos para el diagnóstico y la evaluación del desarrollo de tolerancia. No hallamos estudios publicados sobre la función del índice de neutrófilos/linfocitos (INL) y el volumen plaquetario medio (VPM), que son marcadores no invasivos fácilmente medibles, en pacientes con PAIPD. Objetivos: Determinar la relación entre el INL y el VPM con el diagnóstico y desarrollo de tolerancia en niños con PAIPD. Métodos: Estudio transversal retrospectivo, los datos se obtuvieron del sistema de registros médicos, los síntomas y los resultados de laboratorio de los pacientes con diagnóstico de PAIPD fueron controlados en los consultorios de alergia y gastroenterología. Se compararon valores del hemograma al momento del diagnóstico con el grupo de niños sanos de edad y sexo similares. Resultados: Entre los 59 pacientes con diagnóstico de PAIPD, los varones representaron el 47,4% y las niñas, el 52,6%. El VPM y el volumen plaquetario relativo (VPR) eran significativamente más altos entre los pacientes con PAIPD en comparación con el grupo de referencia (n: 67) (p < 0,001). Asimismo, VPM y el VPR fueron significativamente elevados en pacientes que no desarrollaron tolerancia comparados con los que la desarrollaron (p= 0,01). Con el INL no hubo diferencias entre los grupos. Conclusiones: El VPM y el VPR se consideraron marcadores adecuados para predecir el pronóstico de los pacientes con PAIPD dado que son rápidos, costo-efectivos y fáciles de medir.

Background. Today, as a result of an increase in the frequency of food protein-induced allergic proctocolitis (FPIAP), there is a need for studies not only to enlighten the pathophysiology of the disease but also to determine simple, non-invasive markers in both diagnosis, and evaluation of the development of tolerance. No study has been found in the literature about the place of neutrophil/lymphocyte ratio (NLR) and mean platelet volume (MPV), which are easy to calculate and non-invasive markers. Objectives. The purpose is to determine the relation between NLR and MPV with the diagnosis and development of tolerance in children with FPIAP. Methods. In this retrospective cross-sectional study, clinical, demographic symptoms and laboratory findings of patients, monitored with FPIAP diagnosis in allergy and gastroenterology clinics, were acquired from the patient record system. Hemogram values at the time of diagnosis were compared with the values of healthy children of the same age and gender. Results. Among 59 patients diagnosed with FPIAP, males constitute 47.4% and females constitute 52.6%. MPV and platelet crit (PCT) values were significantly high when compared to the control group (n: 67) in FPIAP cases (p <0.001). Also, MPV and PCT values were significantly high in non-tolerance developing cases when compared to developing ones (p= 0.01). Conclusions. Contrary to NLR, MPV and PCT values have been considered to be good markers in predicting prognosis in cases with FPIAP since they are quick, cost effective and easy to calculate.

Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Proctocolite/complicações , Hipersensibilidade Alimentar/complicações , Inflamação/complicações , Proctocolite/imunologia , Proctocolite/sangue , Biomarcadores , Estudos Transversais , Estudos Retrospectivos , Volume Plaquetário Médio , Hipersensibilidade Alimentar/sangue , Tolerância Imunológica , Inflamação/sangue , Contagem de Leucócitos , Neutrófilos
Pesqui. vet. bras ; 38(2): 345-349, fev. 2018. tab, graf
Artigo em Português | LILACS, VETINDEX | ID: biblio-895580


O contador automático hematológico ABX Micros ESX 60 (Horiba Medical 2012) é analisador hematológico veterinário multi-espécie que realiza 50 contagens por hora, libera 18 parâmetros sanguíneos, além de fazer representações gráficas (histogramas) para leucócitos, hemácias e plaquetas. O objetivo deste trabalho é avaliar o desempenho do referido aparelho em relação à linearidade e carryover, através de controle comercial e de amostras de sangue provenientes da rotina do Laboratório de Patologia Clínica Veterinária. De acordo com resultados é possível afirmar que o presente aparelho possui um excelente coeficiente de linearidade (r2=0,99) nos parâmetros de leucócitos, eritrócitos e plaquetas em relação às diluições estudadas. Em relação aos carryover houve excelente desempenho do aparelho, contudo, houve valores não conformes nos parâmetros de CHCM e VPM em uma das metodologias realizadas que pode ser justificada pela limitação da fórmula que não considera a características do equipamento.(AU)

The automated hematology counter ABX Micros 60 ESX (Horiba Medical 2012) is veterinary hematology analyzer multi-species that carries 50 counts per hour releases 18 blood parameters, in addition to graphical representations (histograms) for leukocytes, erythrocytes and platelets. The objective of this study is to evaluate the performance of the apparatus with respect to linearity and carryover through commercial control and blood samples from the routine of Veterinary Clinical Pathology Laboratory. According to results we can say that this device has excellent linearity coefficient (r2=0.99) in leukocyte parameters, erythrocytes and platelets during that time dilutions. Regarding the carryover was excellent device performance, however, was not in conformity values ​​in the parameters of MCHC and VPM in one of the methodologies made that can be justified by the limited formula that does not consider the equipment characteristics.(AU)

Contagem de Eritrócitos/instrumentação , Testes Hematológicos/instrumentação , Testes Hematológicos/veterinária , Contagem de Leucócitos/instrumentação , Contagem de Plaquetas/instrumentação , Contagem de Células Sanguíneas/veterinária
Braz. j. med. biol. res ; 51(6): e6602, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889101


The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.

Animais , Masculino , Feminino , Gravidez , Ratos , Adaptação Fisiológica/fisiologia , Dieta com Restrição de Proteínas/métodos , Desenvolvimento Fetal/fisiologia , Genitália Feminina/fisiologia , Ureia/sangue , Hemoglobinas/análise , Ganho de Peso/fisiologia , Proteínas/análise , Ratos Wistar , Creatinina/sangue , Albuminas/análise , Contagem de Eritrócitos , Globulinas/análise , Hematócrito , Contagem de Leucócitos
Rev. Soc. Bras. Clín. Méd ; 15(2): 94-98, 20170000. tab
Artigo em Português | LILACS | ID: biblio-875551


Objetivo: Avaliar a associação entre maiores níveis da contagem total de leucócitos periféricos e relação neutrófilos/linfócitos obtidos na admissão hospitalar, bem como a morbimortalidade em pacientes diagnosticados com infarto do miocárdio. Métodos: Avaliamos 276 pacientes com infarto agudo do miocárdio, entre janeiro de 2014 e novembro de 2015. Os dados clínicos e laboratoriais foram obtidos e relacionados a: internação, complicações intra-hospitalares, readmissões e mortalidade em 30 dias pós-internação. Resultados: A idade média foi 61 anos e 66,7% eram homens. Pacientes com complicações, comparados ao grupo sem complicações, eram mais velhos (60,85 vs. 62,54; p=0,053), tinham níveis de leucócitos (10,23 vs. 11,78; p=0,001) e neutrófilos (7,17 vs. 8,38; p=0,020) aumentados, doença multiarterial (38,2% vs. 74,5%; p<0.001) e supradesnivelamento de ST (49,3 vs. 65,1; p=0,039). Dois grupos foram constituídos pela mediana (10,56) dos leucócitos e demonstraram-se incidências mais elevadas de choque cardiogênico (p<0,001) e mortes (p=0,031) no grupo com maior leucocitose. Houve associação significativa entre leucócitos >10,56 e valores superiores de CK-MB (p=0,001) e troponina (p=0,039). Conclusão: Houve associação entre aumento dos níveis de leucócitos e maior incidência de complicações intra-hospitalares, incluindo morte, até 1 mês após alta hospitalar em pacientes com infarto agudo do miocárdio. A contagem de leucócitos mostrou relação mais evidente do que a relação neutrófilos/linfócitos e sua fácil acessibilidade colocá-o como ferramenta útil para determinação do prognóstico em pacientes com infarto do miocárdio.(AU)

Objective: To evaluate the association of higher levels of total peripheral leukocytes count and neutrophil/lymphocyte ratio at admission, as well as morbidity and mortality in patients diagnosed with myocardium infarction. Methods: We evaluated 276 patients with Acute Myocardial Infarction through January 2014 to November 2015. Clinical and laboratory data were obtained and related to hospital stay, inhospital complications, 30 day readmissions and mortality. Results: The average age was 61 years, 66.7% were male. Patients with complications, compared to the group with no complications, are older (60.85 vs 62.54, p=0.053), had increased levels of leucocytes (10.23 vs 11.78, p=0.001) and neutrophils (7.17 vs 8.38, p=0.020) count, multivessel disease (38.2% vs 74.5%, p<0.001) and ST elevation (49.3 vs 65.1, p=0.039). Two groups were formed by leucocyte median, and a higher incidence of cardiogenic shock (p<0.001) and deaths (p=0.031) was shown in the group with higher leuco cytosis. There was significant association between leucocytes >10.56 and higher CKMB (p=0.001) and troponin (p=0.039) values. Conclusion: Our study confirms an association between increased levels of leucocytes count and higher incidence of inhospital complications, including death, up to one month after hospital discharge in patients with acute myocardial infarction. Leucocytes count showed a more evident relation than neutrophil/lymphocyte ratio, and its easy accessibility poses it as a very useful tool to determine prognosis in patients with myocardial infarction.(AU)

Humanos , Masculino , Feminino , Idoso , Contagem de Leucócitos , Infarto do Miocárdio/mortalidade , Morbidade
Clinics ; 72(10): 624-628, Oct. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-890678


OBJECTIVES: Tissue adhesives can be used to prevent pulmonary air leaks, which frequently occur after lung interventions. The objective of this study is to evaluate local and systemic effects of fibrin and cyanoacrylate tissue adhesives on lung lesions in rabbits. METHODS: Eighteen rabbits were submitted to videothoracoscopy + lung incision alone (control) or videothoracoscopy + lung incision + local application of fibrin or cyanoacrylate adhesive. Blood samples were collected and assessed for leukocyte, neutrophil and lymphocyte counts and interleukin-8 levels preoperatively and at 48 hours and 28 days post-operatively. After 28 days, the animals were euthanized for gross examination of the lung surface, and lung fragments were excised for histopathological analysis. RESULTS: Fibrin and cyanoacrylate produced similar adhesion scores of the lung to the parietal pleura. Microscopic analysis revealed uniform low-cellular tissue infiltration in the fibrin group and an intense tissue reaction characterized by dense inflammatory infiltration of granulocytes, giant cells and necrosis in the cyanoacrylate group. No changes were detected in the leukocyte, neutrophil or lymphocyte count at any time-point, while the interleukin-8 levels were increased in the fibrin and cyanoacrylate groups after 48 hours compared with the pre-operative control levels (p<0.01). CONCLUSION: Both adhesive agents promoted normal tissue healing, with a more pronounced local inflammatory reaction observed for cyanoacrylate. Among the serum markers of inflammation, only the interleukin-8 levels changed post-operatively, increasing after 48 hours and decreasing after 28 days to levels similar to those of the control group in both the fibrin and cyanoacrylate groups.

Animais , Masculino , Coelhos , Adesivos Teciduais/uso terapêutico , Adesivo Tecidual de Fibrina/uso terapêutico , Cianoacrilatos/uso terapêutico , Lesão Pulmonar/tratamento farmacológico , Valores de Referência , Toracoscopia/métodos , Fatores de Tempo , Ensaio de Imunoadsorção Enzimática , Distribuição Aleatória , Reprodutibilidade dos Testes , Interleucina-8/sangue , Resultado do Tratamento , Hemodinâmica , Contagem de Leucócitos , Pulmão/efeitos dos fármacos , Pulmão/patologia
Arq. bras. med. vet. zootec. (Online) ; 69(5): 1191-1197, set.-out. 2017. tab, graf
Artigo em Português | LILACS, VETINDEX | ID: biblio-877364


Aparelhos de automação, cujo uso está estabelecido na hematologia dos animais domésticos, ainda não são empregados rotineiramente em felídeos selvagens. Esse estudo objetivou avaliar a técnica automatizada na contagem de células sanguíneas de jaguatiricas (Leopardus pardalis), comparando-a com a técnica manual. Foram coletadas amostras sanguíneas de oito jaguatiricas, que se submeteram à contagem de eritrócitos, leucócitos, plaquetas e à determinação do volume globular pelo método manual padrão e por meio do aparelho BC - 2800 VET® com a configuração para gatos domésticos. Os resultados foram avaliados por meio do teste t para dados pareados, e as técnicas submetidas à análise de correlação de Pearson. A técnica automatizada demonstrou resultados estatisticamente semelhantes para eritrócitos e leucócitos. Apenas para volume globular houve diferença significativa entre as técnicas manual e automatizada, mas a correlação foi alta. Apesar de não haver diferença significativa entre as técnicas para plaquetas, a correlação foi baixa. Conclui-se que o aparelho BC - 2800 VET® com a configuração para gatos domésticos é uma técnica confiável na realização do eritrograma e do leucograma para jaguatiricas. Para a determinação do parâmetro volume globular, o aparelho pode ser utilizado, desde que se faça a correção. Para a contagem de plaquetas, a técnica manual é recomendada.(AU)

Automated equipment, whose use is established in hematology of domestic animals, is not yet routinely used in blood cells count of wildlife, due to lack of studies that validate its use. The purpose of this study was to evaluate the automated technique for blood cells count of ocelots (Leopardus pardalis), comparing it with the manual technique. Blood samples were collected from eight ocelots, which were submitted to counting of erythrocytes, leukocytes, platelets and packed cell volume by the standard manual method and by the device VET ® 2800 BC with the configuration for domestic cats. The results were evaluated using the t test for paired data and the techniques submitted to Pearson correlation. The automated technique showed statistically similar results to erythrocytes and leukocytes. Significant difference was found only for packed cell volume between the manual and automated techniques, but the correlation was high. Although there was no significant difference between the techniques for platelets, the correlation was low. We concluded that the 2800 BC VET ® device with the configuration for domestic cats is a reliable technique in performing the erythrocyte and leukocyte counts for ocelots. The device may be used to determine packed cell volume, provided the correction is made. The manual technique is recommended for the platelet count.(AU)

Animais , Contagem de Eritrócitos , Felidae/sangue , Hematócrito/veterinária , Testes Hematológicos/métodos , Testes Hematológicos/veterinária , Contagem de Leucócitos
J. pediatr. (Rio J.) ; 93(3): 301-307, May.-June 2017. tab
Artigo em Inglês | LILACS | ID: biblio-841354


Abstract Objective: The aim of this study was to verify the association of echocardiogram, ferritin, C-reactive protein, and leukocyte count with unfavorable outcomes in pediatric sepsis. Methods: A prospective cohort study was carried out from March to December 2014, with pediatric critical care patients aged between 28 days and 18 years. Inclusion criteria were diagnosis of sepsis, need for mechanical ventilation for more than 48 h, and vasoactive drugs. Serum levels of C-reactive protein, ferritin, and leukocyte count were collected on the first day (D0), 24 h (D1), and 72 h (D3) after recruitment. Patients underwent transthoracic echocardiography to determine the ejection fraction of the left ventricle on D1 and D3. The outcomes measured were length of hospital stay and in the pediatric intensive care unit, mechanical ventilation duration, free hours of VM, duration of use of inotropic agents, maximum inotropic score, and mortality. Results: Twenty patients completed the study. Patients with elevated ferritin levels on D0 had also fewer ventilator-free hours (p = 0.046) and higher maximum inotropic score (p = 0.009). Patients with cardiac dysfunction by echocardiogram on D1 had longer hospital stay (p = 0.047), pediatric intensive care unit stay (p = 0.020), duration of mechanical ventilation (p = 0.011), maximum inotropic score (p = 0.001), and fewer ventilator-free hours (p = 0.020). Conclusion: Cardiac dysfunction by echocardiography and serum ferritin value was significantly associated with unfavorable outcomes in pediatric patients with sepsis.

Resumo Objetivo: Verificar a associação do ecocardiograma, da ferritina, da proteína C reativa (PCR) e da contagem de leucócitos com desfechos desfavoráveis na sepse pediátrica. Métodos: Estudo de coorte prospectivo, de março a dezembro de 2014, com pacientes críticos pediátricos entre 28 dias e 18 anos. Critérios de inclusão foram diagnóstico de sepse, necessidade de ventilação mecânica (VM) por mais de 48 horas e uso de drogas vasoativas. Avaliaram‐se os níveis séricos PCR, ferritina, contagem de leucócitos, no recrutamento (D0), 24 horas (D1) e 72 horas (D3) após o recrutamento. No D1 e no D3 todos os pacientes foram submetidos a ecocardiograma transtorácico para determinação da Fração de Ejeção (FE) do ventrículo esquerdo. Os desfechos avaliados foram tempo de internação hospitalar e na Unidade de Terapia Intensiva Pediátrica (UTIP); duração da VM; horas livres de VM; duração do uso de inotrópicos; escore de inotrópicos máximo e mortalidade. Resultados: Vinte pacientes completaram o estudo. Ferritina elevada no D0 associou‐se com menor tempo livre de ventilação (p = 0,046) e maior escore de inotrópicos máximo (p = 0,009). A disfunção cardíaca pelo ecocardiograma no D1 relacionou‐se com maior tempo de internação hospitalar (p = 0,047), de UTIP (p = 0,020), VM total (p = 0,011), escore de inotrópicos máximo (p = 0,001) e menor tempo livre de VM (p = 0,020). Conclusão: A disfunção cardíaca pelo ecocardiograma e o valor de ferritina sérica associaram‐se significativamente com desfechos desfavoráveis nos pacientes pediátricos com sepse.

Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Pré-Escolar , Criança , Adolescente , Proteína C-Reativa/análise , Ecocardiografia Doppler , Sepse/diagnóstico , Ferritinas/metabolismo , Ferritinas/sangue , Coração/fisiopatologia , Ecocardiografia , Biomarcadores/sangue , Unidades de Terapia Intensiva Pediátrica , Estudos Prospectivos , Sepse/complicações , Sepse/fisiopatologia , Sepse/sangue , Tempo de Internação , Contagem de Leucócitos
Pesqui. vet. bras ; 37(4): 408-414, Apr. 2017. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-895419


Hematologic analysis provides reliable information on the health status of animals. It is an important variable in the assessment of adaptive and productive capacity of breeds under unfavorable environmental conditions. It is an assessment that combined with other genetic and environmental factors and management can become useful for the future sustainability of mainstream agriculture in a hot environment. Adaptive capacity is affected by some factors such as breed, pregnancy and lactation. Therefore, hematological profile is an important indicator of animal health and production. The objective of this study was to perform the comparative hematological analysis of Santa Inês and Morada Nova breeds (native ewes from Brazil) in all different reproductive stages. Twenty Santa Ines and 20 Morada Nova sheep distributed in a completely randomized design in a split-plot arrangement over time were used. To obtain blood counts, blood samples were collected by jugular venipuncture every 14 days, always in the morning, before the animals were released into the pasture. The Morada Nova breed had higher hemoglobin and total leukocyte count than Santa Inês breed. Regarding the influence of pregnancy and puerperium on the eritrogram, there was an increment in red blood cell, hemoglobin and packed cell volume of both breeds in middle pregnancy. However, had a reduction in late pregnancy. There were a reestablishment of the blood cell counts during the puerperium period. It was verified an increase on neutrophil: lymphocyte ratio indicated that ewes in the late pregnancy, postpartum and puerperium were under stress conditions compared to others reproductive stages. The total plasma proteins also increased during this period to compensate for the high nutritional requirements of the fetus, and these levels remained high until the end of the puerperal phase, when the lambs were weaned. The pregnancy and puerperium influenced all erythrocyte indices and changed the total leukocyte count.(AU)

A análise hematológica fornece informação confiável sobre a saúde dos animais. É uma variável importante na avaliação da capacidade adaptativa e produtiva das raças sob condições ambientais desfavoráveis. É uma avaliação que aliada a outros fatores genéticos, ambientais e de manejo pode se tornar cada vez mais útil para a sustentabilidade futura da agricultura convencional em um ambiente quente. A capacidade adaptativa é afetada por alguns fatores como raça, gestação e lactação. Portanto, o perfil hematológico é um indicador importante da saúde e produção animal. O objetivo deste estudo foi realizar a análise hematológica comparativa de ovelhas Morada Nova e Santa Inês (ovelhas nativas do Brasil) em todos os estágios reprodutivos. Foram utilizadas 20 ovelhas Santa Inês e 20 Morada Nova distribuídas em um delineamento inteiramente casualizado, em parcelas subdivididas e medidas repetidas no tempo. Para a análise do sangue, foram coletadas amostras por venopunção jugular a cada 14 dias, sempre colhidas pela manhã, antes que os animais fossem soltos no pasto. A raça Morada Nova apresentou maior teor de hemoglobina e contagem total de leucócitos que a raça Santa Inês. Em relação à influência da gestação e do puerpério sobre o eritrograma, observou-se que houve um incremento dos valores da contagem dos eritrócitos, hematócrito e hemoglobina até o terço médio da gestação, reduzindo no final da gestação e restabelecendo novamente no período puerperal. Foi verificado um aumento na relação neutrófilo/linfócito indicando que ovelhas no final da gestação, no pós-parto e puerpério estavam sob condição de estresse comparado com os outros estágios reprodutivos. As proteínas plasmáticas totais também aumentaram durante o período gestacional para compensar os altos requisitos nutricionais do feto, e estes níveis permaneceram elevados até ao final da fase de puerpério quando os cordeiros foram desmamados. A gestação e o puerpério influenciaram todos os índices eritrocitários com alteração da contagem total de leucócitos.(AU)

Animais , Feminino , Gravidez , Hemoglobinas/análise , Prenhez/sangue , Ovinos/sangue , Fenômenos Reprodutivos Fisiológicos , Contagem de Leucócitos/veterinária , Testes Hematológicos/veterinária
Braz. j. med. biol. res ; 50(5): e6021, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-839297


Intracranial infection is a common clinical complication after craniotomy. We aimed to explore the diagnostic and prognostic value of dynamic changing procalcitonin (PCT) in early intracranial infection after craniotomy. A prospective study was performed on 93 patients suspected of intracranial infection after craniotomy. Routine peripheral venous blood was collected on the day of admission, and C reactive protein (CRP) and PCT levels were measured. Cerebrospinal fluid (CSF) was collected for routine biochemical, PCT and culture assessment. Serum and CSF analysis continued on days 1, 2, 3, 5, 7, 9, and 11. The patients were divided into intracranial infection group and non-intracranial infection group; intracranial infection group was further divided into infection controlled group and infection uncontrolled group. Thirty-five patients were confirmed with intracranial infection after craniotomy according to the diagnostic criteria. The serum and cerebrospinal fluid PCT levels in the infected group were significantly higher than the non-infected group on day 1 (P<0.05, P<0.01). The area under curve of receiver operating characteristics was 0.803 for CSF PCT in diagnosing intracranial infection. The diagnostic sensitivity and specificity of CSF PCT was superior to other indicators. The serum and CSF PCT levels have potential value in the early diagnosis of intracranial infection after craniotomy. Since CSF PCT levels have higher sensitivity and specificity, dynamic changes in this parameter could be used for early detection of intracranial infection after craniotomy, combined with other biochemical indicators.

Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Calcitonina/sangue , Calcitonina/líquido cefalorraquidiano , Infecções Bacterianas do Sistema Nervoso Central/diagnóstico , Craniotomia/efeitos adversos , APACHE , Biomarcadores/sangue , Biomarcadores/líquido cefalorraquidiano , Proteína C-Reativa/análise , Infecções Bacterianas do Sistema Nervoso Central/sangue , Infecções Bacterianas do Sistema Nervoso Central/líquido cefalorraquidiano , Infecções Bacterianas do Sistema Nervoso Central/microbiologia , Diagnóstico Precoce , Contagem de Leucócitos , Complicações Pós-Operatórias/sangue , Complicações Pós-Operatórias/líquido cefalorraquidiano , Complicações Pós-Operatórias/microbiologia , Prognóstico , Estudos Prospectivos , Valores de Referência , Reprodutibilidade dos Testes , Fatores de Risco , Sensibilidade e Especificidade , Fatores de Tempo
Arq. Inst. Biol ; 84: e0992016, 2017. tab, graf
Artigo em Português | LILACS, VETINDEX | ID: biblio-887871


O presente trabalho teve como objetivo avaliar o estresse e as alterações no leucograma dos juvenis de Oreochromis niloticus submetidos à toxicidade aguda do óleo diesel. A toxicidade foi avaliada em um delineamento inteiramente casualizado com cinco diluições (20, 25, 30, 35 e 40%) e um controle, ambos com três repetições. Para a avaliação dos níveis glicêmicos e alteração no leucograma, foi retirado sangue por punção caudal dos peixes moribundos. Dessa forma, conclui-se que o óleo diesel pode apresentar riscos ao ecossistema aquático, provocando toxicidade aos organismos aquáticos e alterações fisiológicas.(AU)

This study aimed to evaluate the stress and changes in white blood cell count of juveniles Oreochromis niloticus subjected to acute toxicity of diesel oil. The toxicity was evaluated in a completely randomized design with five dilutions (20, 25, 30, 35 and 40%) and one control, both with three replicates. For the assessment of blood glucose levels and changes in white blood cell count, blood was drawn by caudal puncture of the dying fish. It was concluded that diesel oil can present risks to aquatic ecosystem, causing toxicity and physiological changes to fish.(AU)

Animais , Envenenamento/sangue , Óleos Combustíveis , Ciclídeos , Contagem de Leucócitos/veterinária , Substâncias Perigosas , Fenômenos Fisiológicos
Clinics ; 71(10): 611-616, Oct. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-796863


OBJECTIVES: Pleural effusion is a common diagnostic and clinical problem. The differential diagnosis of pleural effusion may be difficult and may require several procedures, including invasive ones. Certain studies have investigated biochemical parameters to facilitate the diagnosis of exudative pleural effusion; however, it remains a challenging problem in clinical practice. We aimed to investigate the potential role of the neutrophil-lymphocyte ratio, which can be easily obtained by determining the cell count of the pleural fluid, in the differential diagnosis of exudative pleural effusion. METHODS: Records from patients who underwent thoracentesis and pleural fluid analysis between May 1, 2013, and March 1, 2015, were obtained from the electronic database of our hospital. The patients who met the inclusion criteria were divided into five groups according to their diagnosis: malignant pleural effusion, para-malignant pleural effusion, para-pneumonic effusion, tuberculosis-related effusion or other. The neutrophil-lymphocyte ratio value was calculated by dividing the absolute neutrophil count by the absolute lymphocyte count. The patient groups were compared according to the given parameter. RESULTS: A total of 465 patients who met the inclusion criteria among 1616 patients with exudative pleural effusion were included in the study. The mean neutrophil-lymphocyte ratio value was significantly lower in tuberculosis-related pleural effusion compared to malignant, para-pneumonic and para-malignant effusions (p=0.001, p=0.001, p=0.012, respectively). The areas under the curve for tuberculosis pleurisy compared to malignant, para-pneumonic and para-malignant effusions were 0.38, 0.36, and 0.37, respectively. Lower cut-off values had higher sensitivity but lower specificity for tuberculosis pleurisy, while higher cut-off values had higher specificity but lower sensitivity for this condition. CONCLUSION: The pleural fluid neutrophil-lymphocyte ratio, which is an inexpensive, reproducible, and easily calculated hematological parameter, may facilitate the differential diagnosis of pleural effusion.

Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Linfócitos , Neutrófilos , Derrame Pleural/sangue , Derrame Pleural/diagnóstico , Biomarcadores/sangue , Diagnóstico Diferencial , Exsudatos e Transudatos , Contagem de Leucócitos , Padrões de Referência , Valores de Referência , Reprodutibilidade dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , Estatísticas não Paramétricas