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1.
Acta cir. bras ; 34(11): e201901103, Nov. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1054680

RESUMO

Abstract Purpose: To evaluate liver regeneration after selective ligation of portal vein and hepatic artery by 3D Computed Tomography in an experimental model. Methods: Sixteen Wistar rats were randomized into four equal groups: Group I- control (sham), Group II- isolated selective ligation of the hepatic artery, Group III- isolated selective ligation of the portal vein and Group IV- combined ligation of portal vein and hepatic artery. Before procedure and five days after a 3D CT Scan was performed to analyze the hypertrophy, weight and function of the remnant liver. Results: The largest regeneration rate and increase of weight in the hypertrophied lobe was detected in group IV, the first with an average of 3.99 (p=0.006) and the last varying from 6.10g to 9.64g (p=0.01). However, total liver weight and the R1 ratio (Hypertrophied Lobe Weight/Total Liver Weight) was higher in group III (P<0.001) when compared with groups I, II and IV and showed no difference between them. The immunohistochemical examination with PCNA also found higher percentages with statistical significance differences in rats of groups III and IV. It was possible to confirm a strong correlation between hypertrophied lobe weight and its imaging volumetric study. Liver function tests only showed a significant difference in serum gamma-glutamyltransferase and phosphorous. Conclusion: There is a largest liver regeneration after combined ligation of portal vein and hepatic artery and this evidence may improve the knowledge of surgical treatment of liver injuries, with a translational impact in anima nobile.


Assuntos
Animais , Masculino , Veia Porta/cirurgia , Artéria Hepática/cirurgia , Fígado/diagnóstico por imagem , Regeneração Hepática/fisiologia , Tamanho do Órgão/fisiologia , Imuno-Histoquímica , Distribuição Aleatória , Tomografia Computadorizada por Raios X/métodos , Reprodutibilidade dos Testes , Resultado do Tratamento , Ratos Wistar , Imageamento Tridimensional/métodos , Hepatomegalia/fisiopatologia , Hepatomegalia/diagnóstico por imagem , Ligadura , Fígado/irrigação sanguínea , Fígado/patologia
2.
Repert. med. cir ; 28(3): 152-156, 2019.
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1046821

RESUMO

La regeneración hepática ha sido uno de los procesos más estudiados en la medicina al ser el hígado un órgano altamente importante y complejo del organismo, asiento de múltiples enfermedades y expuesto por su ubicación anatómica a los traumatismos. El trasplante hepático se ha convertido en uno de los procedimientos más frecuentes en la trasplantología de órganos, por lo que el conocimiento de los aspectos básicos de esta técnica terapéutica y el estudio de los mecanismos histológicos y fisiológicos vinculados con la regeneración hepática es fundamental para lograr un resultado satisfactorio. Se realizó un estudio exploratorio de la bibliografía vinculada con el tema, con el objetivo de exponer aspectos esenciales y actualizados vinculados a las modificaciones fisiológicas y la respuesta orgánica ante el trasplante hepático, además de algunas características clínicas prácticas en este procedimiento.


Liver regeneration has been the focus of intense study in medicine given the liver is a very important and complex organ, affected by multiple illnesses and vulnerable for injury due to its fixed position. Liver transplantation has become one of the most frequent transplantation procedures, thus, knowledge on the basic aspects of this therapeutic technique and the study of the histological and physiological mechanisms linked to liver regeneration is critical to achieve a satisfactory outcome. An exploratory research of the bibliography on this topic was conducted aiming to expose essential and updated aspects related to the physiological modifications and immune response after liver transplantation, and some practical clinical features of this procedure.


Assuntos
Humanos , Regeneração Hepática , Transplante de Fígado
3.
Acta cir. bras ; 34(6): e201900606, 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1019270

RESUMO

Abstract Purpose To investigate the effects of pine needle extract (PNE) on the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 during liver regeneration induced by 70% partial hepatectomy (PH) in rat. Methods Forty-eight male rats (SD, 7 weeks) had surgery (70% PH). They were randomly divided into two groups. PH + PNE group was only provided PNE diluted in water (10%) for drinking and PH group was provided water from 5 days before surgery to the time of sacrifice. PNE was made by pressing and filtering. Animals were sacrificed at 12h, 24h, 36h, 60h, 84h, 168h after PH, respectively. The expressions of PCNA and Ki-67 were determined as proliferation indices. Results Immunohistochemistry turned out to increase the expression of PCNA and Ki-67. PCNA expression of PH+PNE group increased up to twice of that of PH group. Western blot also seemed to increase the PCNA expression. These results indicated the promotion of cell proliferation in liver tissue and hepatic regeneration. Conclusions Pine needle extract stimulates the expression of some mitotic proteins during liver regeneration induced by 70% PH in rats. It suggests that administration of pine needle extract could accelerate the liver regeneration after partial hepatectomy.


Assuntos
Animais , Masculino , Ratos , Extratos Vegetais/farmacologia , Antígeno Nuclear de Célula em Proliferação/efeitos dos fármacos , Antígeno Ki-67/efeitos adversos , Pinus/química , Hepatectomia/métodos , Regeneração Hepática/efeitos dos fármacos , Fatores de Tempo , Ratos Sprague-Dawley , Antígeno Nuclear de Célula em Proliferação/metabolismo , Antígeno Ki-67/metabolismo , Proliferação de Células , Índice Mitótico
4.
Acta cir. bras ; 33(5): 439-445, May 2018. graf
Artigo em Inglês | LILACS | ID: biblio-949344

RESUMO

Abstract Purpose: To investigate the effects of capsiate treatment on hepatic hyperplasia in partially hepatectomized rats. Methods: The animals were divided into a Capsiate group (CPH), a Capsiate Post-Partial Hepatectomy group (CPPH) and a Partial Hepatectomy Control group (PH). CPH and CPPH animals received 60 mg/kg/day Capsiate for 30 days. Next, the rats underwent partial hepatectomy. CPPH animals continued to receive treatment for 48 h after partial hepatectomy. Liver tissue and intracardiac blood samples were obtained 24 or 48 h after PH. Results: Capsiate treatment interfered with hepatic parameters, reducing the number of mitoses and apoptosis and increasing blood ALT and alkaline phosphatase concentrations. Conclusion: Capsiate treatment preceding hepatic surgery may compromise the initial period of postoperative recovery.


Assuntos
Animais , Masculino , Ratos , Capsaicina/análogos & derivados , Hepatectomia , Fígado/enzimologia , Aspartato Aminotransferases/metabolismo , Capsaicina/farmacologia , Ratos Wistar , Apoptose/efeitos dos fármacos , Alanina Transaminase/metabolismo , Fosfatase Alcalina/metabolismo , Fígado/efeitos dos fármacos , Fígado/patologia , Regeneração Hepática/efeitos dos fármacos , Mitose/efeitos dos fármacos
5.
Acta cir. bras ; 33(2): 110-116, Feb. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-886264

RESUMO

Abstract Purpose: To investigate thymoquinone, curcumin and a combination of these two drugs were effective or not at the growth of liver. Methods: Forty female Wistar-Albino rats distributed into five groups of eight rats each, control, thymoquinone, curcumin, and thymoquinone/curcumin groups. Pathological specimens were studied using the Ki-67 Proliferation Index(PI); and arginase(Arg), tissue plasminogen activator(tPA), ceruloplasmin(Cer) and nitric oxide(NO) were studied in biochemical analysis. Results: Our results showed that Ki-67 proliferation index was low in Groups 1. The proliferation coefficient was significantly higher in the Group 2 and Group 4 than in the Group 1 and Group 3.(P < 0.001 between Groups 1 and 2, 1 and 4, and 3 and 4). There was no difference between Groups 2 and 4 (P = 1). The results of the biochemical Arg, tPA and Cer test showed statistically between the Group 1 and Group 2. NO showed significant differences Group 1 and 3. Conclusions: Thymoquinone and curcumin both have known positive effects on the organism. Histological and biochemical tests showed that thymoquinone is more effective than curcumin.


Assuntos
Animais , Feminino , Ratos , Regeneração Hepática/efeitos dos fármacos , Antioxidantes/farmacologia , Arginase/sangue , Ceruloplasmina/análise , Biomarcadores/sangue , Benzoquinonas/farmacologia , Transplante de Fígado , Ativador de Plasminogênio Tecidual/sangue , Ratos Wistar , Antígeno Ki-67/análise , Curcumina/farmacologia , Proliferação de Células , Hepatectomia/métodos , Fígado/patologia , Neoplasias Hepáticas/cirurgia , Antineoplásicos/farmacologia , Óxido Nítrico/sangue
6.
Rev. Col. Bras. Cir ; 44(5): 476-481, Sept.-Oct. 2017. tab
Artigo em Inglês | LILACS | ID: biblio-896617

RESUMO

ABSTRACT Objective: to evaluate whether colectomy, associated with 70% hepatectomy, influences liver regeneration in rats. Methods: we distributed 18 Wistar rats in three groups of six animals each. In group I (sham), we performed laparotomy; In group II, colectomy + 70% hepatectomy; In group III, only 70% hepatectomy. On the 6th postoperative day, we collected blood by cardiac puncture under anesthesia, followed by euthanasia. We performed serum dosages of aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin and alkaline phosphatase (AF), hepatocyte growth factor (HGF) and transforming growth factor-α (TGF-α). We calculated liver regeneration by the formula: liver weight ratio per 100g body weight at the time of euthanasia / liver weight preoperatively projected for 100g body weight × 100. Results: ALT and AST levels were significantly lower in group II when compared with group III (p<0.001). Albuminemia showed significantly higher levels in group II. Levels of HGF and TGF-α in group II were significantly higher than in group III. The percentage of hepatic regeneration was significantly higher in group II than in group III. Conclusion: Colectomy performed simultaneously with 70% hepatectomy had a positive influence on liver regeneration in rats. Further research is needed to reveal the molecular mechanisms of this effect and to characterize the colon influence in liver physiology.


RESUMO Objetivo: avaliar se a colectomia, associada à hepatectomia 70%, influencia a regeneração do fígado em ratos. Métodos: foram utilizados 18 ratos Wistar distribuídos em três grupos de seis animais cada. No grupo I (sham) foi realizada laparotomia; no grupo II colectomia + hepatectomia 70%; no grupo III apenas hepatectomia 70%. No sexto dia pós-operatório foi colhido sangue por punção cardíaca, sob anestesia, seguido de eutanásia. Foram realizadas dosagens séricas de aspartato aminotransferase (AST), alanina aminotransferase (ALT), albumina e fosfatase alcalina (FA), fator de crescimento de hepatócitos (HGF) e fator de crescimento transformador-α (TGF-α). A regeneração do fígado foi calculada pela fórmula: razão peso do fígado por 100g do peso corporal no momento da eutanásia/peso do fígado no pré-operatório projetado por 100g de peso corporal ×100. Resultados: Os níveis de ALT e AST foram significativamente menores no grupo II quando comparados com o grupo III (p<0,001). A albuminemia mostrou níveis significativamente mais elevados no grupo II. Os níveis de HGF e TGF-α no grupo II foram significativamente mais elevados que no grupo III. O percentual de regeneração hepática foi significativamente mais elevado no grupo II do que no grupo III. Conclusão: o estudo demonstrou que a colectomia realizada simultaneamente à hepatectomia 70% influenciou positivamente na regeneração do fígado em ratos. Pesquisas adicionais são necessárias para revelar os mecanismos moleculares deste efeito e para caracterizar a influência do cólon na fisiologia do fígado.


Assuntos
Animais , Masculino , Ratos , Colectomia , Colo , Hepatectomia , Regeneração Hepática , Ratos Wistar
7.
Acta cir. bras ; 32(7): 515-522, July 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-886219

RESUMO

Abstract Purpose: To evaluate the effects of mesenchymal stem cells on liver regeneration in rats following a 70% hepatectomy. Methods: Forty rats were subjected to 70% hepatectomy and then ~106 mesenchymal stem cells (test group), or saline solution (control group), were infused into their livers via the portal vein. Each treatment group was divided into early and late subgroups (euthanized 3 d and 5 d following the operation, respectively). Group comparisons of Albumin, aminotransaminases (AST, ALT), and Alcaline Phosphatase (AP) levels, proliferative index (ki-67+ straining), and mitotic cell counts were conducted. Results: No significant differences in liver regeneration rate, number of mitoses, proliferative index, or serum levels of albumin, AST, or AP were observed. ALT levels were higher in the test group than in the control group (p<.05). Conclusions: Mesenchymal stem-cell therapy did not improve liver regeneration rate 3 d or 5 d after 70% hepatectomy in rats. Likewise, the therapy appeared not to affect liver function, proliferative index, or number of mitoses significantly.


Assuntos
Animais , Masculino , Ratos , Transplante de Células-Tronco Mesenquimais/métodos , Hepatectomia/métodos , Regeneração Hepática , Fatores de Tempo , Ratos Wistar , Modelos Animais , Proliferação de Células , Injeções Intravenosas
8.
Einstein (Säo Paulo) ; 15(2): 178-185, Apr.-June 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-891376

RESUMO

ABSTRACT Objective To analyze the influence of portal vein ligation in hepatic regeneration by immunohistochemical criteria. Methods Ten pigs divided into two groups of five animals underwent hepatectomy in two stages, and the groups were differentiated by ligation or not of the left portal vein tributary, which is responsible for vascularization of the left lateral and medial lobes of the pig liver. Five days after the procedure, the animals underwent liver biopsies for further analysis of histological and immunohistochemical with marker Ki67. Results The group submitted to hepatectomy with vascular ligation showed an increase of approximately 4% of hepatocytes in regeneration status, as well as a greater presence of Kupffer and inflammatory cells as compared to control. Conclusion As a result of positive cell replication observed through the Ki67 marker, we can suspect that the ligation of a tributary of the portal vein associated with liver resection promoted a greater stimulus of liver regeneration when compared to liver resection alone.


RESUMO Objetivo Analisar a influência da ligadura da tributária da veia porta no estímulo regenerativo hepático por meio de critérios imuno-histoquímicos. Métodos Dez suínos, divididos em dois grupos de cinco animais, foram submetidos à hepatectomia em dois estágios, sendo que os grupos foram diferenciados pela ligadura ou não da tributária da veia porta, responsável pela vascularização dos lobos lateral e medial esquerdos do fígado do suíno. Cinco dias após o procedimento, os animais foram reabordados para retirada de amostras hepáticas para posterior análise de histológica e imunoistoquímica com o marcador Ki67. Resultados O grupo submetido à hepatectomia com ligadura vascular apresentou incremento de 4% aproximadamente de hepatócitos em processo de regeneração, bem como grande número de células de Kupffer e células inflamatórias, quando comparado ao controle. Conclusão Em virtude da análise positiva da replicação celular observada por meio do marcador Ki67, pode-se observar que a ligadura de uma tributária da veia porta promoveu um maior estímulo de regeneração hepática, efeito observado com menor intensidade no grupo submetido apenas à ressecção hepática.


Assuntos
Animais , Veia Porta/cirurgia , Tecido Parenquimatoso/cirurgia , Hepatectomia/métodos , Fígado/cirurgia , Regeneração Hepática , Suínos , Distribuição Aleatória , Antígeno Ki-67/metabolismo , Hepatócitos/metabolismo , Modelos Animais , Tecido Parenquimatoso/patologia , Leucócitos , Ligadura/métodos , Fígado/patologia
9.
Acta cir. bras ; 32(5): 396-406, May 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-837708

RESUMO

Abstract Purpose: To determine the effects of propofol and ketamine anesthesia on liver regeneration in rats after partial hepatectomy (PHT). Methods: Male Wistar albino rats were assigned randomly to four groups of 10. Anesthesia was induced and maintained with propofol in groups 1 and 2, and with ketamine in groups 3 and 4. PHT was undertaken in groups 1 and 3. Rats in groups 2 and 4 (control groups) underwent an identical surgical procedure, but without PHT. At postoperative day-5, rats were killed. Regenerated liver was removed, weighed, and evaluated (by immunohistochemical means) for expression of inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), apoptosis protease-activating factor (APAF)-1, and proliferating cell nuclear antigen (PCNA). Also, blood samples were collected for measurement of levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6. Results: Between groups 2 and 4, there were no differences in tissue levels of iNOS, eNOS, and APAF-1 or plasma levels of TNF-α and IL-6. eNOS expression was similar in group 1 and group 3. Expression of iNOS and APAF-1 was mild-to-moderate in group 1, but significantly higher in group 3. Groups 1 and 3 showed an increase in PCNA expression, but expression in both groups was comparable. Plasma levels of TNF-α and IL-6 increased to a lesser degree in group 1 than in group 3. Conclusion: Propofol, as an anesthetic agent, may attenuate cytokine-mediated upregulation of iNOS expression and apoptosis in an animal model of liver regeneration after partial hepatectomy.


Assuntos
Animais , Masculino , Propofol/farmacologia , Apoptose , Anestésicos Intravenosos/farmacologia , Óxido Nítrico Sintase Tipo II/metabolismo , Ketamina/farmacologia , Regeneração Hepática/efeitos dos fármacos , Distribuição Aleatória , Propofol/metabolismo , Regulação para Cima , Interleucina-6/metabolismo , Interleucina-6/sangue , Ratos Wistar , Antígeno Nuclear de Célula em Proliferação/metabolismo , Anestésicos Intravenosos/metabolismo , Modelos Animais , Óxido Nítrico Sintase Tipo III/metabolismo , Fator Apoptótico 1 Ativador de Proteases/metabolismo , Hepatectomia , Ketamina/metabolismo
10.
Acta cir. bras ; 32(4): 263-269, Apr. 2017. graf
Artigo em Inglês | LILACS | ID: biblio-837702

RESUMO

Abstract Purpose: To evaluate the effect of aqueous extract of Baccharis trimera leaves on the proliferative capacity of the liver after partial hepatectomy (PH) in rats. Methods: Twenty Wistar rats weighing between 300 and 450g were divided into two groups: control (HP) and test (HP100-rats that received the aqueous extract of Baccharis trimera for four days at a dose of 100 mg / kg / day). On the fifth day, animals from both groups underwent resection of 70% of the liver. Twenty-four hours later, they were sacrificed and the remnant liver was removed and prepared for studied through PCNA immunohistochemistry. Data analysis for comparison between the two groups was made through the non-parametric statistical test Mann-Whitney test. Results: In all the animals studied was found most abundant nuclear immunostaining positive hepatocytes interlobular located in regions of the liver. Quantitative analysis of PCNA-positive cells revealed positivity rate significantly higher mean (p = 0.02) in HP100 group (77.1 ± 13.6) compared to the HP group (45.8 ± 12.9). Conclusion: DAdministration of aqueous extract of the leaves of Baccharis trimera 100 mg/kg of animal has a significant positive effect on liver regeneration in rats, 24 hours after hepatectomy (70%).


Assuntos
Animais , Ratos , Extratos Vegetais/uso terapêutico , Antígeno Nuclear de Célula em Proliferação/análise , Baccharis , Hepatectomia , Regeneração Hepática/efeitos dos fármacos , Ratos Wistar , Modelos Animais de Doenças , Fígado/efeitos dos fármacos , Fígado/patologia
11.
Acta cir. bras ; 32(3): 194-202, Mar. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-837694

RESUMO

Abstract Purpose: To investigate the effects of lycopene and resveratrol pretreatment on hepatic hyperplasia in partially hepatectomized rats. Methods: The lycopene group and the resveratrol group received 40 mg/kg/day of lycopene or resveratrol, respectively (dissolved in olive oil or in saline solution, respectively) and administered via a gastric tube for 30 days. The partially hepatectomzed (PH) control groups received saline or olive oil via a gastric tube for 30 days, respectively, and the normal control group received no treatment. Liver tissue and intracardiac blood samples were obtained 24, 36 or 48 h after PH. Results: No areas of fibrosis were detected. No significant changes in mitotic index, in the number of apoptosis events or in aspartate aminotransferase and alanine aminotransferase levels were observed. Conclusions: Lycopene and resveratrol pretreatment did not interfere on hepatic hyperplasia in partially hepatectomized rats.


Assuntos
Animais , Masculino , Estilbenos/farmacologia , Carotenoides/farmacologia , Hepatectomia/métodos , Fígado/cirurgia , Fígado/efeitos dos fármacos , Antioxidantes/farmacologia , Aspartato Aminotransferases/sangue , Fatores de Tempo , Reprodutibilidade dos Testes , Resultado do Tratamento , Ratos Wistar , Apoptose/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Alanina Transaminase/sangue , Fígado/enzimologia , Fígado/patologia , Regeneração Hepática/efeitos dos fármacos , Índice Mitótico
12.
Acta cir. bras ; 31(9): 608-614, Sept. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-795998

RESUMO

ABSTRACT PURPOSE: To assess the effect of aqueous extract of Peumus Boldus (AEPB) on the liver proliferative response after parcial hepatectomy of 70% (PH) in rodents. METHODS: Twenty Wistar rats were divided in two groups: AEPB100 (whose rats received 100mg/Kg of AEPB, once a day, orally, in 4 days prior to the first surgical procedure) and Vehicle (whose rats were treated similarly with distilled water). Both groups underwent PH. After 24 hours the remaining livers were removed for studying the proliferation of hepatocytes by Ki-67 and 2mL of blood were collected for serological assessment: cholesterol, glucose, triglycerides, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and total, direct and indirect bilirubin. All data were analyzed by Gaussian distribution. Statistically significant differences between mean values were analyzed using T Student's test. Non-Gaussian data were analyzed using Mann-Whitney's test. RESULTS: The liver of all these rats presented positive staining of Ki-67, indicating liver proliferation. Laboratory results showed no significant difference in serum values between the analyzed groups. The analysis of Ki-67 was significantly more positive in AEPB100 group than in Vehicle group. CONCLUSION: Aqueous extract of Peumus Boldus acute administration exerts significant positive effect on liver regeneration after 24h in rats that underwent parcial hepatectomy, while maintaining unchanged hepatic function.


Assuntos
Animais , Feminino , Extratos Vegetais/farmacologia , Hepatócitos/efeitos dos fármacos , Peumus/química , Hepatectomia/métodos , Fígado/fisiologia , Regeneração Hepática/efeitos dos fármacos , Ratos Wistar , Folhas de Planta/química , Hepatócitos/metabolismo , Modelos Animais de Doenças , Fígado/efeitos dos fármacos
13.
São Paulo; s.n; 2016. [117] p. ilus, tab, graf.
Tese em Português | LILACS | ID: biblio-870877

RESUMO

O transplante hepático é o único tratamento efetivo para uma variedade de doenças hepáticas irreversíveis. No entanto, o número limitado de doadores pediátricos leva ao uso de enxertos hepáticos de doadores adultos, com necessidade de anastomoses vasculares mais complexas. Essas anastomoses tornam-se complicadas pela diferença no calibre dos vasos entre o doador e o receptor, resultando em alterações do fluxo sanguíneo, estenose da anastomose venosa ou arterial e trombose. Os efeitos para regeneração hepática decorrentes da privação do fluxo sanguíneo pela veia porta ou pela artéria hepática não estão completamente elucidados. Experimentalmente, quando um lobo do fígado não recebe o fluxo venoso portal, é observada atrofia deste segmento e hipertrofia do restante do órgão perfundido. Embora existam vários modelos experimentais para estudo da regeneração hepática, poucos são focados em animais em crescimento. Além disso, os efeitos regenerativos de drogas como o tacrolimus e a insulina precisam ser pesquisados, com o objetivo de encontrar um tratamento ideal para a insuficiência hepática ou um método de estimular a regeneração do fígado após ressecções ou transplantes parciais. O objetivo do presente estudo é descrever modelos de regeneração hepática em ratos em crescimento com: 1) ausência de fluxo hepático arterial e 2) redução do fluxo portal. Adicionalmente, o estudo avalia o efeito pró-regenerativo do tacrolimus e da insulina nesses modelos descritos. MÉTODOS: cento e vinte ratos (entre 50 e 100g de peso) foram divididos em 6 grupos, de acordo com o tipo de intervenção cirúrgica: Grupo 1, incisão abdominal sem intervenção hepática; Grupo 2, hepatectomia a 70%; Grupo 3, hepatectomia a 70% + estenose de veia porta; Grupo 4, hepatectomia a 70% + ligadura da artéria hepática; Grupo 5, hepatectomia a 70% + estenose de veia porta + insulina; Grupo 6, hepatectomia a 70% + estenose de veia porta + tacrolimus. Os animais dos grupos 1 ao 4 foram...


Liver transplantation is an effective treatment for a variety of irreversible liver diseases. However, the limited number of pediatric donor livers leads to the use of adult livers, which usually require more complex vascular anastomoses. These anastomoses are complicated by differences in vessel caliber between donors and recipients, resulting in vascular flow anomalies, stenosis of the venous or arterial anastomosis and thrombosis . The effects of portal vein or hepatic arterial flow privation in hepatic regeneration have not been completely elucidated. Experimentally, when a liver lobe is deprived of portal vein flow, atrophy is observed with hypertrophy of the other perfused parts of the organ, and interleukin-6 (IL-6) is required for normal liver regeneration. Although several experimental models are currently used to study the liver regeneration mechanisms, few studies have focused on the growing animal. In addition, the regenerative effects of drugs (e.g., tacrolimus and insulin) have been experimentally studied, aiming to find an ideal treatment for hepatic failure or a method of stimulating liver regeneration after extensive resection or partial transplants. The aim of the present investigation was to describe the new models of liver regeneration in growing rats with: 1) absence of arterial blood hepatic inflow and 2) reduced portal flow. Additionally, it was studied whether tacrolimus or insulin could have any pro-regenerative effect under such conditions. METHODS/MATERIALS: one hundred and twenty rats (50-100 g body weight) were divided into 6 groups based on the intervention type: Group 1 (sham), abdominal incision without intervention; Group 2, 70% hepatectomy; Group 3, 70% hepatectomy + portal vein stenosis; Group 4, 70% hepatectomy + ligation of the hepatic artery; Group 5, 70% hepatectomy + portal vein stenosis + insulin; and Group 6, 70% hepatectomy + portal vein stenosis + tacrolimus. Animals in groups 1 to 4 were...


Assuntos
Animais , Masculino , Ratos , Constrição Patológica , Artéria Hepática , Insulina , Regeneração Hepática , Modelos Animais , Veia Porta , Tacrolimo , Trombose
14.
Rev. Col. Bras. Cir ; 42(6): 393-397, Nov.-Dec. 2015. tab, graf
Artigo em Português | LILACS | ID: lil-771145

RESUMO

Objective: to evaluate liver regeneration in rats after partial hepatectomy of 60% with and without action diet supplemented with fatty acids through the study of the regenerated liver weight, laboratory parameters of liver function and histological study. Methods: thirty-six Wistar rats, males, adults were used, weighing between 195 and 330 g assigned to control and groups. The supplementation group received the diet by gavage and were killed after 24h, 72h and seven days. Evaluation of regeneration occurred through analysis of weight gain liver, serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidase, and mitosis of the liver stained with H&E. Results: the diet supplemented group showed no statistical difference (p>0.05) on the evolution of weights. Administration of fatty acids post-hepatectomy had significant reduction in gamma glutamyltransferase levels and may reflect liver regeneration. Referring to mitotic index, it did not differ between period of times among the groups. Conclusion: supplementation with fatty acids in rats undergoing 60% hepatic resection showed no significant interference related to liver regeneration.


Objetivo: avaliar a regeneração hepática em ratos submetidos à hepatectomia parcial de 60% com e sem ação de dieta suplementada com ácidos graxos ômega-3 através do estudo ponderal do fígado regenerado, parâmetros laboratoriais da função hepática e estudo histológico. Métodos: foram usados 36 ratos machos, distribuídos em dois grupos: grupo controle e grupo ômega-3. Cada um foi subdividido em mais três subgrupos com óbito em 24h, 72h e sete dias. O grupo ômega-3 recebeu água e dieta padrão suplementada com emulsão lipídica de ácidos graxos ômega-3 a 10% e o controle solução fisiológica a 0,9%. Em todos os subgrupos foi feita análise da regeneração hepática através da fórmula de Kwon, estudo da função hepática: dosagem de AST, ALT, gama-GT, bilirrubina total, bilirrubina indireta e indireta e albumina, e análise de mitose celular pela coloração de Hematoxilina-Eosina. Resultados: o grupo com dieta suplementada não apresentou diferença estatística (p>0,05) quanto à evolução dos pesos. Administração de ácidos graxos ômega-3 pós-hepatectomia mostrou que os níveis de gama-GT tiveram redução significante, podendo refletir na regeneração hepática. Na avaliação do índice mitótico não houve diferença entre os momentos estudados. Conclusão: a suplementação com ácidos graxos ômega-3 em ratos submetidos à ressecção hepática a 60% não apresentou papel expressivo relacionados à regeneração do fígado.


Assuntos
Animais , Masculino , Ratos , Ácidos Graxos Ômega-3 , Dieta , Regeneração Hepática , Ratos Wistar , Hepatectomia , Fígado
15.
Arq. bras. cardiol ; 104(6): 443-449, 06/2015. tab
Artigo em Inglês | LILACS | ID: lil-750700

RESUMO

Background: The aging process promotes a progressive increase in chronic-degenerative diseases. The effect of these diseases on the functional capacity has been well recognized. Another health parameter concerns “quality of life related to health”. Among the elderly population, cardiovascular diseases stand out due to the epidemiological and clinical impact. Usually, these diseases have been associated with others. This set of problems may compromise both independence and quality of life in elderly patients who seek cardiologic treatment. These health parameters have not been well contemplated by cardiologists. Objective: Evaluating, among the elderly population with cardiovascular disease, which are the most relevant clinical determinants regarding dependence and quality of life. Methods: This group was randomly and consecutively selected and four questionnaires were applied: HAQ, SF-36, PRIME-MD e Mini Mental State. Results: The study included 1,020 elderly patients, 63.3% women. The group had been between 60 and 97 years-old (mean: 75.56 ± 6.62 years-old). 61.4% were independent or mild dependence. The quality of life total score was high (HAQ: 88.66 ± 2.68). 87.8% of patients had a SF-36 total score > 66. In the multivariate analysis, the association between diagnoses and high degrees of dependence was significant only for previous stroke (p = 0.014), obesity (p < 0.001), lack of physical activity (p = 0.016), osteoarthritis (p < 0.001), cognitive impairment (p < 0.001), and major depression (p < 0.001). Analyzing the quality of life, major depression and physical illness for depression was significantly associated with all domains of the SF-36. Conclusion: Among an elderly outpatient cardiology population, dependence and quality of life clinical determinants are not cardiovascular comorbidities, especially the depression. .


Fundamento: Com o envelhecimento, a prevalência de doenças crônico-degenerativas sofreu aumento progressivo. A repercussão dessas doenças sobre a capacidade funcional foi reconhecida. Outro parâmetro de saúde é a “qualidade de vida relacionada à saúde”. Na população idosa, as doenças cardiovasculares destacam-se pelo impacto epidemiológico e clínico. Elas, geralmente, vêm associadas a outras afecções. Esse conjunto de problemas pode comprometer a independência e a qualidade de vida do idoso que busca tratamento cardiológico. Objetivo: Avaliar, em uma população de idosos cardiopatas, quais são os determinantes clínicos mais relevantes de dependência e de qualidade de vida. Métodos: O grupo foi selecionado aleatória e consecutivamente, sendo aplicados quatro questionários: HAQ, SF-36, PRIME‑MD e Mini Exame do Estado Mental. Resultados: Incluiu-se 1020 idosos, 63,3% mulheres. O grupo tinha em média 75,56 ± 6,62 anos. 61,4% mostrou-se independente ou com dependência leve. O escore de qualidade de vida foi elevado (HAQ: 88,66 ± 2,68). 87,8% dos pacientes apresentou escore total do SF-36 ≥ 66. À análise multivariada, a associação entre os diagnósticos e graus elevados de dependência foi significante apenas para acidente vascular cerebral prévio (p = 0,014), obesidade (p < 0,001), sedentarismo (p = 0,016), osteoartrite (p < 0,001), déficit cognitivo (p < 0,001), e depressão maior (p < 0,001). Ao analisarmos a qualidade de vida, a depressão maior e a depressão por doença física associou-se significativamente com todos os domínios do SF-36. Conclusão: Em uma população de idosos cardiopatas, os determinantes clínicos mais relevantes de prejuízos para dependência e qualidade de vida foram as comorbidades não cardiovasculares, particularmente a depressão. .


Assuntos
Humanos , Hepatócitos/patologia , Regeneração Hepática , Falência Hepática Aguda/metabolismo , Apoptose , /fisiologia , Proteína Ligante Fas/fisiologia , Hepatócitos/metabolismo , Falência Hepática Aguda/terapia , Necrose , Receptores do Fator de Necrose Tumoral/metabolismo , Transdução de Sinais , Ligante Indutor de Apoptose Relacionado a TNF/fisiologia , Fator de Necrose Tumoral alfa/metabolismo
16.
Rev. chil. cir ; 67(2): 185-190, abr. 2015. ilus, graf, tab
Artigo em Espanhol | LILACS | ID: lil-745080

RESUMO

Introduction: Hepatic resection is the standard treatment for the liver tumors and in most cases the only potentially curative option. The most feared complication, in the major hepatic resection, is the postoperative liver failure. A new technique has recently been published in the literature: Liver partition associated to a portal vein ligation for staged hepatectomy. This technique allows an accelerated hipertrophy of the remnant liver, facilitates greater resection surgery, reducing the risk of postoperative hepatic ischemia. The results reported in the literature are promising. We report the first case in Chile with its evolution to 2 years and a discussion about the topic.


La resección hepática es el tratamiento estándar para los tumores hepáticos malignos y en la mayoría de los casos la única opción potencialmente curativa. La complicación más temida, en las grandes resecciones, es la insuficiencia hepática postquirúrgica. Una nueva técnica ha sido recientemente publicada en la literatura: La partición hepática asociada a la ligadura de la vena portal para realizar una hepatectomía por etapas. Esta técnica permite una hipertrofia acelerada del hígado remanente que posibilita una cirugía resectiva mayor, disminuyendo el riesgo de isquemia hepática postquirúrgica. Los resultados expuestos en la literatura son promisorios. Presentamos el primer caso clínico en Chile con su evolución a 2 años y una discusión del tema.


Assuntos
Humanos , Adulto , Feminino , Hepatectomia/métodos , Regeneração Hepática , Neoplasias Hepáticas/cirurgia , Veia Porta/cirurgia , Ligadura
17.
Acta cir. bras ; 30(2): 100-106, 02/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-741021

RESUMO

PURPOSE: To evaluate which is the best route of administration for cell therapy in experimental rat model of small-for size syndrome. METHODS: A total of 40 rats underwent partial hepatectomy (70%) that induces the small-for-size syndrome and were divided into four groups of route administration: intravenous, intraperitoneal, enteral and tracheal. The small-for-size syndrome model was designed with extended partial hepatectomy (70%). The animals were divided into four groups of routes administration: intravenous (n=10) - intravenously through the dorsal vein of the penis; intraperitoneal (n=10) - intraperitoneally in the abdominal cavity; enteral (n=10) - oroenteral with the placement of a number 4 urethral probe and maintained at third duodenal portion; tracheal (n=10) - after tracheal intubation. We track the animals and monitor them for 21 days; during this follow-up we evaluated the result of cell therapy application tracking animals using ultrasound, radiography and PET-scan. Statistical analysis was performed using GraphPad Prism Software(r). Differences were considered significant with the p<0.05. Data are presented as the median and variation for continuous variables. Comparisons between groups were made using analysis of the imaging test by the researchers. RESULTS: All four groups underwent partial hepatectomy of 70% liver tissue targeting the same weight of resected liver. Initially the PET-scan tests showed similarity in administered cells by different routes. However, in few days the route of intravenous administration showed to be the most appropriated to lead cells to the liver followed by enteral. The tracheal and peritoneal routes were not as much successful for this goal. CONCLUSION: The intravenous route is the best one to cell therapy in experimental rat model of small-for size-syndrome. .


Assuntos
Animais , Masculino , Modelos Animais de Doenças , Vias de Administração de Medicamentos , Hepatopatias/terapia , Regeneração Hepática/fisiologia , Transplante de Células-Tronco/métodos , Terapia Baseada em Transplante de Células e Tecidos/métodos , Hepatectomia , Transplante de Fígado/efeitos adversos , Fígado/química , Tamanho do Órgão , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Síndrome , Fatores de Tempo
18.
Acta cir. bras ; 30(1): 54-59, 01/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-735707

RESUMO

PURPOSE: To evaluate the influence of the cyclosporine in liver regeneration in rats submitted to an experimental model of 70% hepatectomy. METHODS: Forty male rats were randomly divided in four subgroups (C.24h, C.7d, E.24h, E.7d), according to the drug used and the day of sacrifice (24 hours and 7 days). Cyclosporine (10mg/Kg/day) was given to the study subgroup and 1 ml of 0.9% sodium chloride was to the control subgroup. Resection of left lateral lobe and median lobe performing 70% of liver mass. During the animals' death, KWON formula was applied. Counting of mitotic figures and percentage of positive nucleus with PCNA and Ki-67 were evaluated. RESULTS: In the 2nd, 4th PO and death days, E.7d lose more weight than C.7d. Regarding to the KWON formula, the C.7d regenerated more than the C.24h and the same with the E.7d. Comparing between the groups, only E7d subgroup was statistically significant compared with C.7d, showing the stimulating effect of cyclosporine in liver regeneration. Immunohistochemestry had significant results between the study subgroups. The mitotic index revealed statistical differences in the control subgroups. CONCLUSION: Cyclosporine, in spite of being an immunosuppressive drug, has a positive effect in liver regeneration, although reduce the animal's body weight. .


Assuntos
Animais , Masculino , Ciclosporina/farmacologia , Hepatectomia/métodos , Imunossupressores/farmacologia , Regeneração Hepática/efeitos dos fármacos , Peso Corporal , Contagem de Células , Proliferação de Células , Modelos Animais de Doenças , Imuno-Histoquímica , Índice Mitótico , Antígeno Nuclear de Célula em Proliferação/análise , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Fatores de Tempo , Resultado do Tratamento
19.
Lambayeque; s.n; 2015. 30 p. graf, tab.
Tese em Espanhol | LILACS, MOSAICO - Saúde integrativa | ID: biblio-910766

RESUMO

El consumo de alcohol provoca la enfermedad alcohólica hepática la cual engloba a una serie de patologías iniciando con una infiltración grasa, posterior inflamación hepática, fibrosis, cinosis y eventual carcinoma hepatocelular. La prevalencia de estos cambios genera gastos en el sistema salud y malestar en el paciente. Se estudió la histología de la regeneración hepática de ratas que consumieron alcohol crónicamente tratadas con infusión de flor de overo (Cordia lutea). Ratas (Rattus norvegicus, cepa Sprague-Dawley) (n=18), pesando 192.6 +- 26.74g fueron divididas en tres grupos (n=06) y tratadas durante 120 días: Grupo I, consumió agua ad libitum; Grupo II, consumió alcohol 4% v/v; Grupo m, consumió alcohol 4% v/v y 21 días de infusión de flor de overo. Los hígados fueron pe:rfundidos, fijados y cortes teñidos con eosinahematoxilina. El consumo medio de alcohol de los grupos ll y llI fue estadísticamente similar (p>0.05), media global = 3.14 +- 0.44 g/kg/día. La degeneración hepática grasa fue estadísticamente mayor (p<0.05) en el grupo ll que en los grupos I y llI donde esta fue similar (p>0.05). El consumo medio de flor de overo fue 222.14 +- 20.4 mg/kg/día. El consumo de infusión de flor de overo regeneró el hígado graso de ratas que consumieron alcohol crónicamente.


Assuntos
Animais , Ratos , Cordia , Fígado Gorduroso Alcoólico , Regeneração Hepática , Técnicas Histológicas , Modelos Animais , Peru
20.
Arch. méd. Camaguey ; 18(5): 519-531, sep.-oct. 2014. ilus, tab
Artigo em Espanhol | LILACS, CUMED | ID: lil-723750

RESUMO

FUNDAMENTO: la regeneración hepática es la respuesta fundamental del hígado frente a los diversos estímulos internos y externos. Es multifactorial inducida, que origina cambios secuenciales en la expresión génica, la estructura y organización de las células hepáticas. OBJETIVOS: analizar el comportamiento de la densidad superficial de los núcleos de los hepatocitos según la posición que ocupan en las zonas del lóbulo hepático medio de ratones sometidos a citosol regenerativo. Método: se estudió la densidad superficial de los núcleos de hepatocitos con el uso de la morfometría en 14 lóbulos medios de hígados de ratones, que fueron sometidos a dosis de citosol de hígado regenerativo al inicio del experimento. Las muestras fueron tomadas cada 12 horas durante ocho días. El corte se dividió en zonas proximal, medial y distal al hilio donde se realizaron las determinaciones. RESULTADOS: los valores promedios y su desviación estándar en la zonas fueron: proximal 701,23 ± 135,83 (μm2.103), medial 707,48 ± 46,87 (μm2.103) y distal 682,48 ± 125,21 (μm2.103). Al comparar los resultados mediante el análisis de varianza se aprecia que no existen diferencias en el comportamiento de los promedios en las tres zonas. Al comparar la fase inicial con la fase final del experimento se aprecia diferencias significativas (p ≤ 0,05) en las mismas zonas, con un predominio de la zona proximal y distal. CONCLUSIONES: en las tres zonas del lóbulo el comportamiento de la densidad superficial de núcleos de hepatocitos ocurre de manera similar, con un sentido al incremento al final del experimento, lo que se corroboró con la aplicación de la prueba de la t de Student. Los incrementos más significativos ocurrieron en la zona proximal y distal, aunque en todas las zonas existen diferencias significativas entre los valores obtenidos en la primera mitad de tiempo del experimento respecto a la parte final.


BACKGROUND: hepatic regeneration is the basic answer of the liver to various internal and external stimuli. It is multifactorial and induced and originates sequential changes in the gene expression, the structure and organization of hepatic cells. OBJECTIVE: to analyze the behaviour of the superficial density of the nuclei of the hepatocytes according to the position they have in the areas of the middle hepatic lobes of mice subjected to regenerative cytosol. METHOD: the superficial density of the nuclei of the hepatocytes was studied with the use of morphometry in 14 middle hepatic lobes of mice subjected to a dose of regenerative liver cytosol at the beginning of the experiment. The samples were taken every 12 hours during eight days. The cut was divided into proximal, medial and distal areas to the hilum, where the determinations were made. Results: the average values and their standard deviations in the areas were: proximal 701,23 ± 135,83 (μm2.103), medial 707,48 ± 46,87 (μm2.103) y distal 682,48 ± 125,21 (μm2.103). When comparing the results through the analysis of variance, it could be seen that there are not differences among the averages in the three areas. When comparing the initial stage with the final stage of the experiment major differences (p ≤ 0,05) could be seen in the same areas, predominating the proximal and distal area. CONCLUSIONS: in the three areas of the lobe the behaviour of the superficial density of the nuclei of the hepatocytes is similar, with a tendency to increase at the end of the experiment. This could be corroborated with the application of the Student’s t- test. In spite of the fact that there were major differences between the values obtained in the first part and the final part of the experiment in all the areas, the most significant increases occurred in the proximal and distal areas.


Assuntos
Animais , Ratos , Hepatócitos/citologia , Regeneração Hepática/fisiologia , Animais de Laboratório , Contagem de Células
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