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Braz. j. microbiol ; 49(1): 13-15, Jan.-Mar. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889194


ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149T comprises 9,165,825 bp with G + C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites. The presence of these biosynthetic gene clusters in MUSC 149T suggests the potential exploitation of the strain for production of medically important compounds.

Streptomyces/isolamento & purificação , Genoma Bacteriano , Sedimentos Geológicos/microbiologia , Filogenia , Streptomyces/classificação , Streptomyces/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Composição de Bases , DNA Bacteriano/genética , Dados de Sequência Molecular , Sequência de Bases , Malásia
Braz. j. microbiol ; 49(1): 10-12, Jan.-Mar. 2018. tab
Artigo em Inglês | LILACS | ID: biblio-889198


ABSTRACT Vitellibacter aquimaris D-24T (=KCTC 42708T = DSM 101732T), a halophilic marine bacterium, was isolated from seawater collected from Desaru beach, Malaysia. Here, we present the draft genome sequence of D-24T with a genome size of approximately 3.1 Mbp and G + C content of 39.93%. The genome of D-24T contains genes involved in reducing a potent greenhouse gas (N2O) in the environment and the degradation of proteinaceous compounds. Genome availability will provide insights into potential biotechnological and environmental applications of this bacterium.

Água do Mar/microbiologia , Genoma Bacteriano , Flavobacteriaceae/genética , Filogenia , Composição de Bases , DNA Bacteriano/genética , Dados de Sequência Molecular , Sequência de Bases , Flavobacteriaceae/isolamento & purificação , Flavobacteriaceae/classificação , Malásia
Braz. J. Pharm. Sci. (Online) ; 54(3): e17552, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-974395


The incidence of erectile dysfunction (ED) is rising worldwide and its prevalence is one of the main health concerns that affect overall men well-being in Malaysia. The cluster of demographic, clinical and lifestyle factors may have contributed to the severity of ED and changes in biomarkers level; nevertheless these have not been studied extensively. This cross sectional study involved a total of 276 patients with 138 was diagnosed with ED. The demographic, clinical, lifestyle factors and severity of ED were assessed using a set of questionnaire and the International Index of Erectile Function (IIEF-5). Meanwhile, Total Testosterone (TT) and Asymmetric dimethylarginine (ADMA) levels were determined using the enzyme-linked immunosorbant assay (ELISA). Binary logistic regression test was used to demonstrate the predictors of severity of ED, TT and ADMA levels. Significant predictors for worsening of severity of ED are self-employed [10.55 (0.43 - 257.06), p=0.004], pensioner [8.07 (0.19 - 352.45), p=0.026], non-government employee [1.16 (0.05 - 26.26), p=0.04] and TT [0.41 (0.25 - 0.69), p=0.001]. Nevertheless, pensioner [0.08 (0.01 - 0.87), p=0.038] and unemployed [0.04 (0.01 - 0.42), p=0.007], were the predictors that may influence the changes of TT levels. On the other hand, academic qualification (secondary) [4.51 (0.48 - 42.83), p=0.014] and intensity of physical activities (< 1 hour/day) [2.61 (0.65 - 10.48), p=0.008] were the predictors which were more likely to influence the changes of ADMA levels in ED patients. TT and ADMA levels were influenced by demographic and lifestyle factors whilst severity of ED was predicted by demographic and clinical factors in Malaysian ED population. These predictors may provide new knowledge on risk factors of severity of ED and help in management of ED. Thus, the predictive models could serve as a primary guidance to physicians to ensure ED being managed and treated more effectively.

Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Idoso , Disfunção Erétil/prevenção & controle , Malásia/etnologia , Demografia/estatística & dados numéricos , Saúde do Homem/etnologia , Estilo de Vida
Braz. J. Pharm. Sci. (Online) ; 54(4): e17324, 2018. tab
Artigo em Inglês | LILACS | ID: biblio-1001586


This study aims to assess the perceived practice and barriers towards the provision of asthma management services among urban community pharmacists in Selangor, Malaysia. The study also highlights both pharmacist and patient-related barriers in asthma counseling. One hundred fifty urban community pharmacists in Selangor, Malaysia, were randomly selected and recruited for the present cross sectional baseline study. Previous studies have explored pharmacists' perception on their roles in asthma management in different parts of the world. The data was collected through self-explanatory questionnaires (containing 47 items). The extracted data from the completed questionnaires were analyzed descriptively and inferentially using Statistical Package for Social Science (SPSS) version 19. Results showed that pharmacists perceived their roles in asthma management along three major dimensions: 'patient self-management', 'medication use' and 'asthma control', mean (±SD) score of the perception towards asthma management was 99.29 ± 4.58 over a maximum possible score of 110 with 90.26%. There were significant differences between the pharmacy education level and perceived practice of asthma management while there were insignificant differences between age, gender, ethnicity, number of practicing year and perceived practice of asthma management among respondents. Most common barriers towards asthma counselling including lack of time, lack of asthma knowledge, lack of counselling space and cost of asthma drugs. Conclusively, urban community pharmacists in Selangor, Malaysia, demonstrate good perceived practice of better management of asthma with multidimensional dimensional role against in disease care.

Humanos , Masculino , Feminino , Farmacêuticos/ética , Asma/tratamento farmacológico , Controle de Doenças Transmissíveis/normas , Malásia/etnologia , Asma/prevenção & controle
Braz. J. Pharm. Sci. (Online) ; 54(2): e17484, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-951942


Abstract The risk of having microvascular complication is high among Type 2 Diabetes Mellitus (T2DM) patients. However, factors associated with the glycemic control and progression of diabetic retinopathy (DR) in T2DM patients is limited. This study aims to determine association between anti-diabetic agents, glycemic control and progression of diabetic retinopathy in a Malaysian population. A retrospective study conducted in a tertiary teaching hospital in Malaysia, from January 2009 until March 2014. This study enrolled 104 patients aged 40-84 years, with a mean age 63.12 ± 9.18 years. patients had non-proliferative diabetic retinopathy (NPDR, 77%) and 35% had proliferative diabetic retinopathy (PDR). Diabetic macula edema (DME) was present in 20% of NPDR patients, compared with 7% in PDR. Alpha-glucosidase inhibitor (p=0.012), age (p=0.014) and number of antidiabetic agents used (p=0.015) were significantly associated with stages of diabetic retinopathy. Family history of T2DM (p=0.039) was associated with DME. Identifying factors influencing the progression of diabetic retinopathy may aid in optimizing the therapeutic effects of anti-diabetic agents in T2DM patients.

Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Diabetes Mellitus Tipo 2/complicações , Retinopatia Diabética/diagnóstico , Índice Glicêmico , Malásia/etnologia
Arch. argent. pediatr ; 115(3): 212-219, jun. 2017. tab
Artigo em Inglês, Espanhol | LILACS, BINACIS | ID: biblio-887315


Introducción. El agotamiento emocional es un componente importante del síndrome de burnout (desgaste profesional). El burnout es común entre los médicos. Afecta su salud física y mental, su rendimiento y la calidad de la atención que brindan. Este estudio tuvo como propósito investigar el nivel y los factores asociados con el agotamiento emocional en los médicos pediatras, en Malasia. Población y métodos. Eneste estudiomulticéntrico y transversal se utilizó un cuestionario autoadministrado, que incluía preguntas acerca de las características sociodemográficas y laborales, el agotamiento emocional, la escala de estrés percibido y las fuentes laborales de estrés. Con el software SPSS, se llevaron a cabo análisis descriptivos, univariantes y multivariantes. Resultados. Un total de 197 médicos de los departamentos de pediatría de ocho hospitales respondieron el cuestionario. El 25,4% y el 24,4% de los médicos informaron, respectivamente, agotamiento emocional alto y moderado. En el análisis bivariante, 29 de las 38 opciones correspondientes a fuentes de estrés mostraron una asociación importante con el agotamiento emocional (p < 0,05). En el análisis multivariante, los predictores importantes de agotamiento emocional fueron: puntajes más altos en la escala del estrés percibido, abordaje de problemas psicosociales de los pacientes, falta de cortesía en las interacciones con colegas/subordinados, falta de reconocimiento de parte de los superiores, falta de incentivos y promociones, trabajo bajo presión del tiempo y necesidad de cumplir con los plazos, y establecimiento de metas inaccesibles o autoimposición de ese tipo de metas (p < 0,05). La fuente de estrés mencionada con mayor frecuencia fue el trato con padres difíciles (80,2%). Conclusiones. El agotamiento emocional está asociado con fuentes de estrés en el entorno laboral pero no con factores sociodemográficos.

Introduction. Emotional exhaustion is an important component of burnout. Burnout is common among doctors. It affects the physical and mental health of doctors, their performance and the quality of care they provide. This study aimed to investigate the level and factors associated with emotional exhaustion among doctors in pediatric practice in Malaysia. Population and methods. A self-administered questionnaire was used in this multicenter cross-sectional study. It included questions on the socio-demographics, work characteristics, Emotional Exhaustion, Perceived Stress Scale and sources of job stress. Descriptive, univariate and multivariate analysis were conducted using the SPSS software. Results. A total of 197 doctors working in the Pediatric department in eight hospitals returned complete questionnaire. High and moderate emotional exhaustion was reported by 25.4% and 24.4% doctors, respectively. In bivariate analysis, 29 out of the 38 items of sources of stress showed significant association with emotional exhaustion (p <0.05).The significant predictors of emotional exhaustion in the multivariate analysis were: scoring higher on the Perceived Stress Score, dealing with patient's psychosocial problems, disrespectful interactions with colleagues/subordinates, lack of appreciation from supervisors, lack of incentives and promotions, time pressures and deadlines to meet, and setting unrealistic goals of having them imposed on oneself (p <0.05). The most common source of stress was dealing with difficult parents (80.2%). Conclusions. Emotional exhaustion is associated with sources of stress in the workplace but not with socio-demographic factors.

Humanos , Masculino , Feminino , Adulto , Pediatria , Esgotamento Profissional/etiologia , Estudos Transversais , Emoções , Autorrelato , Hospitais Públicos , Malásia
Rev. Inst. Med. Trop. Säo Paulo ; 59: e4, 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-842774


ABSTRACT Paederus fuscipes, a vector of Paederus dermatitis in most tropical and subtropical countries of the world have a high prevalence in human dwellings due to their positively phototaxic behaviour which has caused a tremendous impact on human health. In this paper, P. fuscipes dispersal flights were studied for two seasons of the rice cultivation phases in residential premises built close to rice field areas (≈32-60 m and 164 m) in mainland Penang, Malaysia. We examined the effects of different light illuminance, building floor level and their association with rice stages as a focal cause of P. fuscipes dispersion from the rice fields towards human dwellings. The present study showed a significant interaction between different light illuminances and rice cultivation phases in attracting P. fuscipes to disperse and invade human dwellings. The highest number of P. fuscipes was captured near the bright light. P. fuscipes flights increased in line with each floor level, and the highest captures took place at higher building floor levels (levels 2 and 3) compared to lower building floor levels (ground floor and level 1) of a three storey apartment in both rice seasons. This finding not only conveys a better understanding on P. fuscipes dispersal pattern, but also draws public attention on the occurrence of dermatitis linearis caused by the Paederus beetles.

Humanos , Animais , Comportamento Animal/fisiologia , Besouros/fisiologia , Dermatite de Contato/parasitologia , Distribuição Animal , Luz , Malásia , Oryza , Densidade Demográfica , Estações do Ano
Trends psychiatry psychother. (Impr.) ; 38(1): 14-22, Jan.-Mar. 2016. tab
Artigo em Inglês | LILACS | ID: lil-779110


Introduction Pediatric anxiety disorders are the most common mental health disorders in the middle-childhood age group. The purpose of this study is to assess anxiety disorder symptoms, as defined by the Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV), in a large community sample of low socioeconomic level rural children and to investigate some of the psychometric properties (internal consistency, construct and convergent validity and items rated as often or always experienced) of the Malay version of the Spence Children's Anxiety Scale - Child version (SCAS-C). Method Six hundred children aged 9-11 and 424 of their parents completely answered the child or parent versions of the SCAS. Results Results indicated that the internal reliability of subscales were moderate to adequate. Significant correlations between child and parent reports supported the measure's concurrent validity. Additionally, anxiety levels in this Malaysian sample were lower than among South-African children and higher than among their Western peers. There were both similarities and differences between symptom items reported as often or always experienced by Malaysian students and by children from other cultures. Confirmatory factor analysis provided evidence of the existence of five inter-correlated factors for anxiety disorders based on SCAS-C. Conclusion Although some of the instrument's psychometric properties deviated from those observed in some other countries, it nevertheless appears to be useful for assessing childhood anxiety symptoms in this country.

Introdução Transtornos de ansiedade são a doença de saúde mental mais comum em crianças na terceira infância. O objetivo deste estudo foi examinar sintomas de transtorno de ansiedade conforme definidos na 4ª edição do Manual Diagnóstico e Estatístico de Transtornos Mentais (DSM-IV) em uma grande amostra comunitária de crianças de baixo nível socioeconômico vivendo em zona rural na Malásia e investigar as propriedades psicométricas (consistência interna, construto, validade convergente e frequência de respostas muitas vezes e sempre) da versão malaia da Escala de Ansiedade Infantil de Spence - Versão para Crianças. Método Um total de 600 crianças com idade entre 9 e 11 anos e 424 pais responderam todas as questões das versões da escala para crianças e para pais. Resultados Os resultados indicaram que a confiabilidade interna das subescalas variou de moderada a suficiente. Correlações significativas entre os relatos das crianças e de seus pais reforçaram a validade convergente do instrumento. O nível de ansiedade observado na amostra de crianças malaias foi menor do o nível relatado para crianças na África do Sul e maior do que em crianças de países ocidentais. A frequência das respostas escolhidas pelos estudantes malaios demonstrou algumas similaridades e diferenças em relação a outras culturas. A análise fatorial confirmatória revelou evidência da presença de cinco fatores inter-relacionados para transtorno de ansiedade com base na escala avaliada. Conclusão Embora algumas propriedades psicométricas do instrumento avaliado tenham desviado dos resultados obtidos em outros países, a escala parece útil para avaliar sintomas de ansiedade na infância na Malásia.

Humanos , Masculino , Feminino , Criança , Transtornos de Ansiedade/diagnóstico , Escalas de Graduação Psiquiátrica , Pais , Psicometria , População Rural , Fatores Socioeconômicos , Fatores Sexuais , Reprodutibilidade dos Testes , Análise Fatorial , Fatores Etários , Manual Diagnóstico e Estatístico de Transtornos Mentais , Malásia
Säo Paulo med. j ; 133(6): 502-509, Nov.-Dec. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-770155


ABSTRACT CONTEXT AND OBJECTIVE: Chronic kidney disease (CKD) is an escalating medical and socioeconomic problem worldwide. Information concerning the causes of CKD, which is a prerequisite for reducing the disease burden, is sparse in Malaysia. Therefore, this study aimed to evaluate the attributable causes of CKD in an adult population at a tertiary referral hospital. DESIGN AND SETTING: Retrospective study at Hospital Universiti Sains Malaysia (HUSM). METHODS: This was an analysis based on medical records of adult patients at HUSM. Data regarding demographics, laboratory investigations, attributable causes and CKD stage were gathered. RESULTS: A total of 851 eligible cases were included. The patients' mean age was 61.18 ± 13.37 years. CKD stage V was found in 333 cases (39.1%) whereas stages IV, IIIb, IIIa, and II were seen in 240 (28.2%), 186 (21.9%), 74 (8.7%) and 18 (2.1%), respectively. The percentage of CKD stage V patients receiving renal replacement therapy was 15.6%. The foremost attributable causes of CKD were diabetic nephropathy (DN) (44.9%), hypertension (HPT) (24.2%) and obstructive uropathy (9.2%). The difference in the prevalence of CKD due to DN, HPT and glomerulonephritis between patients ≤ 50 and > 50 years old was statistically significant. CONCLUSION: Our results suggest that DN and HPT are the major attributable causes of CKD among patients at a Malaysian tertiary-care hospital. Furthermore, the results draw attention to the possibility that greater emphasis on primary prevention of diabetes and hypertension will have a great impact on reduction of hospital admissions due to CKD in Malaysia.

RESUMO CONTEXTO E OBJETIVO: Doença renal crônica (DRC) é um problema médico e socioeconômico crescente. As informações relativas às causas da DRC são pré-requisito para reduzir a carga da doença, e são escassas na Malásia. Este estudo tem como objetivo avaliar as causas atribuíveis à DRC na população adulta de um hospital de referência terciária. TIPO DE ESTUDO E LOCAL: Estudo retrospectivo realizado no Hospital Universiti Sains Malaysia (HUSM). MÉTODOS: Análise de prontuários de pacientes adultos de HUSM. Foram obtidos dados demográficos, exames laboratoriais, causas atribuíveis e estágio da DRC. RESULTADOS: Um total de 851 casos elegíveis foi incluído. A idade média dos pacientes foi de 61,18 ± 13,37 anos. DRC fase V foi contabilizada em 333 casos (39,1%), enquanto casos de estágio IV, IIIb, IIIa e II foram 240 (28,2%), 186 (21,9%), 74 (8,7%) e 18 (2,1%), respectivamente. A porcentagem de pacientes com DRC estágio V recebendo a terapia de substituição renal foi 15,6%. As causas atribuíveis mais importantes da DRC foram nefropatia diabética (ND) (44,9%), hipertensão (24,2%) e uropatia obstrutiva (9,2%). A diferença na prevalência da DRC devido à ND, hipertensão e glomerulonefrite entre pacientes ≤ 50 anos e > 50 anos de idade foi estatisticamente significativa. CONCLUSÃO: Nossos resultados sugerem que a ND e a hipertensão são as principais causas atribuíveis da DRC em pacientes sob cuidados terciários na Malásia. Os resultados apontam para a possibilidade de maior ênfase na prevenção primária da diabetes e hipertensão como impactante na redução das internações hospitalares devidas a DRC na Malásia.

Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Insuficiência Renal Crônica/epidemiologia , Insuficiência Renal Crônica/etiologia , Distribuição por Idade , Nefropatias Diabéticas/complicações , Nefropatias Diabéticas/epidemiologia , Taxa de Filtração Glomerular , Hipertensão/complicações , Hipertensão/epidemiologia , Registros Médicos , Malásia/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Índice de Gravidade de Doença , Distribuição por Sexo , Fatores Sexuais , Centros de Atenção Terciária/estatística & dados numéricos , Obstrução Ureteral/complicações , Obstrução Ureteral/epidemiologia
Trends psychiatry psychother. (Impr.) ; 37(1): 37-41, Jan-Mar/2015. tab
Artigo em Inglês | LILACS | ID: lil-742992


Introduction: Anxiety among children has increased in recent years. Culturally adapted questionnaires developed to measure the level of anxiety are the best screening instruments for the general population. This study describes the scientific translation and adaptation of the Spence Children's Anxiety Scale (SCAS) into the Malay language. Method: The process of scientific translation of this selfreport instrument followed the guidelines of the Task Force for Translation and Cultural Adaptation of the International Society for Pharmacoeconomics and Outcomes Research (ISPOR). Results: The Malay version and its adaptation for a new cultural context are described. Conclusion: The Malay version achieved the aims of the original version and its conceptual and operational equivalence. It may be used as the first Malay instrument to measure anxiety among children in research and in clinical and community settings. .

Introdução: A ansiedade infantil tem crescido em anos recentes. Questionários culturalmente adaptados e desenvolvidos para medir o nível de ansiedade são os melhores instrumentos de triagem para a população em geral. Este estudo descreve a tradução e adaptação científica da Escala de Ansiedade Infantil de Spence para a língua malásia. Método: O processo de tradução científica deste instrumento de autorrelato seguiu as orientações da Força-Tarefa para a Tradução e Adaptação Cultural da International Society for Pharmacoeconomics and Outcomes Research (ISPOR). Resultados: A versão malásia e sua adaptação para um novo contexto cultural são descritas. Conclusão: A versão malásia atingiu os objetivos da versão original e sua equivalência conceitual e operacional. Poderá ser usada como o primeiro instrumento malásio para medir ansiedade entre crianças em contextos de pesquisa, clínicos ou comunitários. .

Humanos , Masculino , Feminino , Criança , Transtornos de Ansiedade/diagnóstico , Escalas de Graduação Psiquiátrica , Inquéritos e Questionários , Cultura , Idioma , Malásia , Autorrelato , Traduções
Braz. j. microbiol ; 45(4): 1385-1391, Oct.-Dec. 2014. ilus, tab
Artigo em Inglês | LILACS | ID: lil-741291


An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.

Técnicas Bacteriológicas/métodos , Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Salmonella typhi/isolamento & purificação , Febre Tifoide/diagnóstico , Primers do DNA/genética , Malásia , Sensibilidade e Especificidade , Salmonella typhi/genética , Fatores de Tempo , Febre Tifoide/microbiologia
Braz. j. microbiol ; 44(4): 1169-1172, Oct.-Dec. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-705280


A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable number and polymerase chain reaction (MPN-PCR) method was employed in this study. It was found that L. monocytogenes detected in 33.33% of the beef offal samples. The prevalence of L. monocytogenes in beef offal purchased from wet markets A, B, and C were 22.73%, 37.50% and 41.18% respectively. The density of L. monocytogenes in all the samples ranged from<3upto> 2,400 MPN/g. The findings in this study indicate that beef offal can be a potential vehicle of foodborne listeriosis.

Animais , Bovinos , Microbiologia de Alimentos , Listeria monocytogenes/isolamento & purificação , Carga Bacteriana , Malásia , Reação em Cadeia da Polimerase , Prevalência
J. pediatr. (Rio J.) ; 89(5): 434-443, set.-out. 2013. tab
Artigo em Português | LILACS | ID: lil-690066


OBJETIVO: Determinar se três variantes (388 G>A, 521 T>C, 463 C>A) do membro 1B1 da família de transportadores de ânions orgânicos portadores de solutos (SLCO1B1) se associam à hiperbilirrubinemia neonatal. FONTE DE DADOS: Foi realizada busca na Infraestrutura do Conhecimento Nacional da China e em MEDLINE. A revisão sistemática com metanálise incluiu estudos genéticos que avaliaram a associação entre hiperbilirrubinemia neonatal e as variantes 388 G>A, 521 T>C, 463 C>A de SLCO1B1 entre janeiro de 1980 e dezembro de 2012. Foi realizada seleção e extração de dados por dois analistas, de forma independente. SUMÁRIO DOS ACHADOS: Foram incluídos dez artigos no estudo. Os resultados revelaram que SLCO1B1 388 G>A se associa a um aumento do risco de hiperbilirrubinemia neonatal (OR< 1,39; IC 95%: 1,07 a 1,82) em recém-nascidos chineses, mas não em recém-nascidos caucasianos, tailandeses, latino-americanos ou malaios. A mutação SLCO1B1 521 T>C mostrou baixo risco de hiperbilirrubinemia neonatal em recém-nascido chineses, e não foram encontradas associações importantes no Brasil nem em recém-nascidos caucasianos, asiáticos, tailandeses e malaios. Não houve diferenças significativas da SLCO1B1 463 C>A entre o grupo com hiperbilirrubinemia e o grupo controle. CONCLUSÃO: O estudo mostrou que a mutação 388 G>A do gene SLCO1B1 é fator de risco para desenvolver hiperbilirrubinemia neonatal em recém-nascidos chineses, mas não em populações caucasianas, tailandesas, brasileiras ou malaias; a mutação SLCO1B1 521 T>C fornece proteção de hiperbilirrubinemia neonatal em recém-nascidos chineses, mas não nas populações caucasianas, tailandesas, brasileiras ou malaias.

OBJECTIVE: To determine whether three variants (388 G>A, 521 T>C, and 463 C>A) of the solute carrier organic anion transporter family member 1B1 (SLCO1B1) are associated with neonatal hyperbilirubinemia. DATA SOURCE: The China National Knowledge Infrastructure and MEDLINE databases were searched. The systematic review with meta-analysis included genetic studies which assessed the association between neonatal hyperbilirubinemia and 388 G>A, 521 T>C, and 463 C>A variants of SLCO1B1 between January of 1980 and December of 2012. Data selection and extraction were performed independently by two reviewers. SUMMARY OF THE FINDINGS: Ten articles were included in the study. The results revealed that SLCO1B1 388 G>A is associated with an increased risk of neonatal hyperbilirubinemia (OR, 1.39; 95% CI, 1.07-1.82) in Chinese neonates, but not in white, Thai, Latin American, or Malaysian neonates. The SLCO1B1 521 T>C mutation showed a low risk of neonatal hyperbilirubinemia in Chinese neonates, while no significant associations were found in Brazilian, white, Asian, Thai, and Malaysian neonates. There were no significant differences in SLCO1B1 463 C>A between the hyperbilirubinemia and the control group. CONCLUSION: This study demonstrated that the 388 G>A mutation of the SLCO1B1 gene is a risk factor for developing neonatal hyperbilirubinemia in Chinese neonates, but not in white, Thai, Brazilian, or Malaysian populations; the SLCO1B1 521 T>C mutation provides protection for neonatal hyperbilirubinemia in Chinese neonates, but not in white, Thai, Brazilian, or Malaysian populations.

Humanos , Recém-Nascido , Hiperbilirrubinemia Neonatal/genética , Transportadores de Ânions Orgânicos/genética , Polimorfismo Genético/genética , Grupo com Ancestrais do Continente Asiático/genética , Brasil , Estudos de Casos e Controles , Estudos de Coortes , China/etnologia , Grupo com Ancestrais do Continente Europeu/genética , Genótipo , Malásia , Mutação/genética , Fatores de Risco , Tailândia , Turquia , Estados Unidos
Braz. j. microbiol ; 44(3): 717-722, July-Sept. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-699804


This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".

Animais , Abelhas/microbiologia , Lactobacillus/classificação , Lactobacillus/isolamento & purificação , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Lactobacillus/genética , Malásia , Dados de Sequência Molecular , Filogenia , /genética , Análise de Sequência de DNA , Estômago/microbiologia
Braz. j. microbiol ; 44(3): 959-968, July-Sept. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-699827


Crown disease (CD) is infecting oil palm in the early stages of the crop development. Previous studies showed that Fusarium species were commonly associated with CD. However, the identity of the species has not been resolved. This study was carried out to identify and characterize through morphological approaches and to determine the genetic diversity of the Fusarium species. 51 isolates (39%) of Fusarium solani and 40 isolates (31%) of Fusarium oxysporum were recovered from oil palm with typical CD symptoms collected from nine states in Malaysia, together with samples from Padang and Medan, Indonesia. Based on morphological characteristics, isolates in both Fusarium species were classified into two distinct morphotypes; Morphotypes I and II. Molecular characterization based on IGS-RFLP analysis produced 27 haplotypes among the F. solani isolates and 33 haplotypes for F. oxysporum isolates, which indicated high levels of intraspecific variations. From UPGMA cluster analysis, the isolates in both Fusarium species were divided into two main clusters with the percentage of similarity from 87% to 100% for F. solani, and 89% to 100% for F. oxysporum isolates, which was in accordance with the Morphotypes I and II. The results of the present study indicated that F. solani and F. oxysporum associated with CD of oil palm in Malaysia and Indonesia were highly variable.

Arecaceae/microbiologia , Fusarium/citologia , Fusarium/genética , Variação Genética , Doenças das Plantas/microbiologia , Análise por Conglomerados , Fusarium/classificação , Fusarium/isolamento & purificação , Haplótipos , Indonésia , Malásia , Microscopia , Tipagem Molecular , Técnicas de Tipagem Micológica , Polimorfismo de Fragmento de Restrição
Clinics ; 68(2): 159-166, 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-668801


OBJECTIVES: Variations in the prevalence of sex-hormone-related diseases have been observed between Asian ethnic groups living in the same country; however, available data concerning their sex hormone levels are limited. The present study aimed to determine the influence of ethnicity and age on the sex hormone levels of Malay and Chinese men in Malaysia. METHODS: A total of 547 males of Malay and Chinese ethnicity residing in the Klang Valley Malaysia underwent a detailed screening, and their blood was collected for sex hormones analyses. RESULTS: Testosterone levels were normally distributed in the men (total, free and non-sex hormone-binding globulin (SHBG) bound fractions), and significant ethnic differences were observed (p<0.05); however, the effect size was small. In general, testosterone levels in males began to decline significantly after age 50. Significant ethnic differences in total, free and non-SHBG bound fraction estradiol levels were observed in the 20-29 and 50-59 age groups (p<0.05). The estradiol levels of Malay men decreased as they aged, but they increased for Chinese men starting at age 40. CONCLUSIONS: Small but significant differences in testosterone levels existed between Malay and Chinese males. Significant age and race differences existed in estradiol levels. These differences might contribute to the ethnic group differences in diseases related to sex hormones, which other studies have found in Malaysia.

Adulto , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Grupo com Ancestrais do Continente Asiático , Estradiol/sangue , Globulina de Ligação a Hormônio Sexual/análise , Testosterona/sangue , Distribuição por Idade , Fatores Etários , Ensaio de Imunoadsorção Enzimática , Métodos Epidemiológicos , Etnologia , Grupos Étnicos/etnologia , Malásia/etnologia
Braz. j. med. biol. res ; 45(12): 1119-1126, Dec. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-659658


The common variants in the fat mass- and obesity-associated (FTO) gene have been previously found to be associated with obesity in various adult populations. The objective of the present study was to investigate whether the single nucleotide polymorphisms (SNPs) and linkage disequilibrium (LD) blocks in various regions of the FTO gene are associated with predisposition to obesity in Malaysian Malays. Thirty-one FTO SNPs were genotyped in 587 (158 obese and 429 non-obese) Malaysian Malay subjects. Obesity traits and lipid profiles were measured and single-marker association testing, LD testing, and haplotype association analysis were performed. LD analysis of the FTO SNPs revealed the presence of 57 regions with complete LD (D’ = 1.0). In addition, we detected the association of rs17817288 with low-density lipoprotein cholesterol. The FTO gene may therefore be involved in lipid metabolism in Malaysian Malays. Two haplotype blocks were present in this region of the FTO gene, but no particular haplotype was found to be significantly associated with an increased risk of obesity in Malaysian Malays.

Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Predisposição Genética para Doença/genética , Desequilíbrio de Ligação/genética , Obesidade/genética , Polimorfismo de Nucleotídeo Único/genética , Proteínas/genética , Índice de Massa Corporal , Estudos de Casos e Controles , Genótipo , Malásia/etnologia , Obesidade/etnologia
Clinics ; 67(8): 911-916, Aug. 2012. tab
Artigo em Inglês | LILACS | ID: lil-647795


OBJECTIVES: Variations in sex hormones and the calcium balance can influence bone health in men. The present study aimed to examine the relationship between the calcaneal speed of sound and biochemical determinants of bone mass, such as sex hormones, parathyroid hormones and serum calcium. METHODS: Data from 549 subjects from the Malaysian Aging Male Study, which included Malay and Chinese men aged 20 years and older residing in the Klang Valley, were used for analysis. The subjects' calcaneal speed of sound was measured, and their blood was collected for biochemical analysis. Two sets of multiple regression models were generated for the total/bioavailable testosterone and estradiol to avoid multicollinearity. RESULTS: The multiple regression results revealed that bioavailable testosterone and serum total calcium were significant predictors of the calcaneal speed of sound in the adjusted model. After adjustment for ethnicity and body mass index, only bioavailable testosterone remained significant; the total serum calcium was marginally insignificant. In a separate model, the total testosterone and sex hormone-binding globulin were significant predictors, whereas the total serum calcium was marginally insignificant. After adjustment for ethnicity and body mass index (BMI), the significance persisted for total testosterone and SHBG. After further adjustment for age, none of the serum biochemical determinants was a significant predictor of the calcaneal speed of sound. CONCLUSION: There is a significant age-dependent relationship between the calcaneal speed of sound and total testosterone, bioavailable testosterone and sex hormone-binding globulin in Chinese and Malay men in Malaysia. The relationship between total serum calcium and calcaneal speed of sound is ethnicity-dependent.

Adulto , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Calcâneo/fisiologia , Cálcio/sangue , Osteoporose/diagnóstico , Som , Globulina de Ligação a Hormônio Sexual/análise , Testosterona/sangue , Índice de Massa Corporal , Densidade Óssea/fisiologia , China , Estradiol/sangue , Malásia , Osteoporose/sangue , Osteoporose/fisiopatologia , Valor Preditivo dos Testes , Estatísticas não Paramétricas
Int. j. morphol ; 29(4): 1321-1325, dic. 2011. ilus
Artigo em Inglês | LILACS | ID: lil-627009


Clinical practice in our country showed a lack of suitable implants that perfectly match the anthropometry of the local population. However, there have been no reports or comprehensive studies conducted to substantiate this argument. We therefore prospectively studied 120 hips from sixty subjects with an average age of 25+/-5.18 years old. The average weight and height were 61.48+/-13.84 kg and 1.65+/-9.63 m respectively. The exclusion criteria were pregnant woman, those who had injury to the hip joint, and those with implant or prosthesis. Four parameters were measured ­ the femoral head diameter (FHD), femoral neck length (FNL), femoral neck width (FNW) and collo-diaphyseal angle (CDA). Statistical analysis showed no significant differences between left and right femora but significant differences were found between male and female subjects. The results for the male and female femurs are as follows ­ FHD: 43.6+/-3.1 mm and 38.9+/-2.2 mm; FNL: 91.1+/-5.7 mm and 81.8+/-4.3 mm; FNW: 28.9+/-3.4 mm and 26.0+/-4.3 mm; CDA: 132.3°+/-3.4° and 129.9°+/-4.0°. This data could be used as a guideline to design a more suitable implant for the Malay population which covers most of the South East Asian countries.

Problemas con respecto al tamaño de los implantes en relación a cada población se han convertido en una cuestión esencial para la sociedad ortopédica. Varios factores han sido identificados en la literatura, los que pueden afectar la estabilidad del implante, especialmente en el fémur proximal. Se estudió prospectivamente 120 caderas mediante imágenes de tomografía computarizada. Sesenta sujetos fueron reclutados, edad media 25+/-5,18 años, peso de 61,48+/-13,84 kg y altura de 1,65+/-9,63m. Los criterios de exclusión para este estudio incluyeron a mujeres embarazadas, quienes hubiesen experimentado lesiones cadera, uso de implantes o prótesis. Nuestros datos se compararon usando las pruebas de Anderson-Darling y t-test. Los parámetros medidos fueron diámetro de la cabeza femoral (FHD), longitud del cuello femoral (FNL), ancho del cuello femoral (FNW) y el ángulo cuello-diafisial (CDA). Los resultados de los fémures masculinos y femeninos fueron los siguientes: FHD 43,6 +/- 3,1 mm y 38,9 +/- 2,2 mm, FNL 91,1 +/- 5,7 mm y 81,8 +/- 4,3 mm, FNW 28,9 +/- 3,4 mm y 26,0 +/- 4,3 mm; CDA 132.3 +/- 3.4 y 129.9 +/- 4,. Este estudio prospectivo y transversal centrado en la población malaya, proporcionó información esencial sobre el valor normal de la porción proximal del fémur, datos que mejorarán el conocimiento de los aspectos anatómicos y, finalmente, ayuden al diseño del vástago femoral en la artroplastía total de cadera (ATC). Los resultados proporcionarán una nueva comprensión por parte del cirujano ortopédico.

Humanos , Masculino , Feminino , Adulto , Fêmur/anatomia & histologia , Fêmur , Tomografia Computadorizada por Raios X , Malásia , Estudos Prospectivos