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1.
Stomatologiia (Mosk) ; 99(3): 18-21, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32608943

RESUMO

The article is devoted to the study of immunohistochemical parameters in patients with bullous lesions of the oral mucosa. The biopsy samples of the oral mucosa were studied in 57 patients, including patients with pemphigoid bullosa (38 people) and pemphigus vulgaris (19 people). The results of immunohistochemical studies indicate the cellular mechanisms of damage to the epithelium, in which IL-1, IL-6 and HPV16 are involved.


Assuntos
Penfigoide Bolhoso , Pênfigo , Vesícula , Humanos , Mucosa Bucal
2.
Stomatologiia (Mosk) ; 99(3): 37-40, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32608947

RESUMO

OBJECTIVE: To study changes in the parameters of endogenous intoxication using the level of substances of low and medium molecular weight in the oral fluid in patients with catarrhal stomatitis, which got developed after the acute drug poisoning. MATERIAL AND METHODS: To determine the degree of intoxication, we studied the concentrations of low and medium molecular weight substances (SLMMW) in the oral fluid of patients. We studied the saliva of 20 patients (13 men and 7 women, average age 39 years). The control was done against the oral fluid of 10 healthy individuals. The studies were conducted in a control group consisting of 10 healthy individuals with intact oral mucosa and two groups of patients (20 patients in total) with drug poisoning. The first of these two groups of patients included 8 individuals without pathological changes in the oral mucosa, while the second group consisted of 12 patients diagnosed with catarrhal stomatitis that developed after acute drug poisoning. RESULTS: It became known that endogenous intoxication develops in the oral fluid of patients with acute drug poisoning, which is manifested by a statistically significant increase in the content of SLMMW in comparison with practically healthy individuals (15.3%). And the total level of SLMMW in the oral fluid of patients with acute drug stomatitis and catarrhal stomatitis has increased by 42.3% relative to the data of practically healthy individuals. CONCLUSION: Studies have shown the need for monitoring the oral cavity in patients with acute drug poisoning, with the aim of timely detection of pathologies of the oral mucosa in them and the appointment of complex treatment.


Assuntos
Resfriado Comum , Estomatite , Transtornos Relacionados ao Uso de Substâncias , Adulto , Feminino , Humanos , Masculino , Mucosa Bucal , Saliva
3.
Braz Dent J ; 31(3): 344-348, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32667521

RESUMO

Caliber persistent labial artery (CPLA) consists in a dilated portion of the main branch of the labial artery without loss of size. The aim of this study is to report a case of a patient diagnosed with CPLA in the upper lip, emphasizing unusual histopathological and immunohistochemical findings. A 67-year-old female patient with complaint of a pulsating upper lip lesion without painful symptomatology. Under a clinical diagnosis of CPLA, and considering that the patient was edentulous and used a total prosthesis, an excisional biopsy of the lesion was performed to avoid future traumas in the region and consequently possible exuberant local bleeding. At anatomopathological examination structures suggestive of lymphoid follicles and germinal centers were visualized. Immunohistochemistry showed positivity for CD20, CD68, desmin and CD34 and negativity for CD4. The patient did not have a history of allergies, cardiovascular, rheumatic or systemic diseases that could justified the findings. The case presents unusual histopathological structures, evidencing the necessity of more studies about this pathology so scarce in the literature.


Assuntos
Doenças Labiais , Malformações Vasculares , Idoso , Artérias , Biópsia , Feminino , Humanos , Lábio , Mucosa Bucal
4.
Braz Dent J ; 31(3): 310-318, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32667525

RESUMO

Oral mucositis is a common inflammatory complication among patients with cancer. This study evaluated the histopathological, stereological, and antioxidant markers of 2% eucalyptus extract in induced oral mucositis in male golden hamsters. In this animal study, oral mucositis was induced in 30 male golden hamsters by 5-FU (60 mg/kg) on days 0, 5, and 10 of the study. The cheek pouch was scratched with a sterile needle once daily on days 3 and 4. On days 14-17, 2% eucalyptus hydroalcoholic extract gel and Calendula officinalis extract gel groups were treated and then compared with a non-treated control group. The histopathological and stereological scores and the pouch content of malondialdehyde, as well as the activities of glutathione and myeloperoxidase in the pouch tissue, were evaluated. Histopathologic scores of oral mucositis were lower in the eucalyptus gel group than those of the calendula and control groups (p<0.05). Also, a lower malondialdehyde level and higher myeloperoxidase and glutathione activities were detected in the eucalyptus group in comparison to the calendula and control groups (p<0.001). The thickness of the mucosa and submucosa increased in the eucalyptus group. The numerical density of the fibroblast and the volume density of the collagen significantly increased in the eucalyptus group. In conclusion, the use of eucalyptus hydroalcoholic extract may be associated with reduced intensity of oral mucositis, diminished concentration of malondialdehyde, increased activity of myeloperoxidase and glutathione, increased volume of mucosa and submucosa, increased fibroblast and collagen in the induced oral mucositis in golden hamsters undergoing 5-FU consumption.


Assuntos
Eucalyptus , Mucosite , Estomatite , Animais , Cricetinae , Fluoruracila , Masculino , Mesocricetus , Mucosa Bucal , Extratos Vegetais
5.
Int J Infect Dis ; 97: 326-328, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32526392

RESUMO

Some oral manifestations have been observed in patients with coronavirus disease 2019 (COVID-19). However, there is still a question about whether these lesions are due to coronavirus infection or secondary manifestations resulting from the patient's systemic condition. Thus, this article aims to report an additional case of an oral condition in a patient diagnosed with COVID-19. Our patient, a sixty-seven-year-old Caucasian man, tested positive to coronavirus and presented oral manifestations such as recurrent herpes simplex, candidiasis, and geographic tongue. We support the argument that some oral conditions could be secondary to the deterioration of systemic health or due to treatments for COVID-19. The present case report highlights the importance of including dentists in the intensive care unit multi-professional team to improve oral health in critical patients, not only COVID-19 patients, but also, to contribute to evidence-based and decision-making in managing infectious diseases.


Assuntos
Infecções por Coronavirus/patologia , Mucosa Bucal/patologia , Pneumonia Viral/patologia , Idoso , Betacoronavirus , Humanos , Masculino , Mucosa Bucal/virologia , Pandemias
6.
J Med Microbiol ; 69(6): 824-829, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32478655

RESUMO

Introduction. Candida auris is an emerging fungal pathogen. The organism can cause invasive infections associated with high mortality, has been implicated in outbreaks in healthcare settings and is frequently resistant to multiple antifungal agents, making it a significant challenge to infection prevention and patient treatment.Aim. To implement a real-time PCR assay for detection of C. auris in patient surveillance samples collected with the Copan Liquid Amies elution swab (ESwab) collection and transport system.Methodology. We optimized a real-time PCR testing procedure based on the sample collection device used in our institution.Results . ESwab transport medium was strongly inhibitory to the real-time PCR. Removing the medium with centrifugation, followed by suspending the pellet in PBS-BSA buffer (concentration 1 %), sufficiently eliminated the inhibition. The manual sample preparation method, freeze-thaw followed by mechanical disruption, allowed the detection of C. auris at the lowest cell concentration.Conclusion . The optimized procedure was used to test 1414 patient surveillance samples. The real-time PCR detected all culture-positive samples with 100 % sensitivity and 100 % specificity.


Assuntos
Axila/microbiologia , Candida/isolamento & purificação , Virilha/microbiologia , Mucosa Bucal/microbiologia , Reação em Cadeia da Polimerase em Tempo Real/métodos , Candida/genética , Humanos , Sensibilidade e Especificidade
7.
J Contemp Dent Pract ; 21(4): 390-395, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32584274

RESUMO

AIM: Lichen planus is an autoimmune disease that can affect the skin and mucous membranes, including the oral mucosa. Vitamin D plays a very important role in the bone health along with boosting immunity. This study was carried out to assess the role of vitamin D in the treatment of oral lichen planus. MATERIALS AND METHODS: A sample size of 450 was taken which included patients showing clinical presentation of oral lichen planus. The patients were equally divided into three different groups based on their vitamin D levels. The data were analyzed using SPSS version 26.0. RESULTS: The patients who were given the vitamin D supplementation showed the maximum improvement. The comparison of the data were found to be statistically significant. CONCLUSION: It was concluded that vitamin D played an important role in the treatment of oral lichen planus. CLINICAL SIGNIFICANCE: The role of vitamin D in lichen planus needs to be explored. This study may be useful in conducting further study to assess the role in vitamin D supplementation in the treatment of oral lichen planus.


Assuntos
Líquen Plano Bucal , Líquen Plano , Humanos , Mucosa Bucal , Pele , Vitamina D
8.
Indian J Dent Res ; 31(2): 241-246, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32436904

RESUMO

Background: The palatal rugae contribute to oral swallowing, taste perception, and speech. From a forensic point of view, the distinctive morphology of these structures can be used to support human identification. However, the morphology of the rugae may be altered by trauma or therapeutic interventions in the palate. Objective: This study aimed to analyze the morphology of the palatal rugae before and after orthodontic treatment performed with and without maxillary expansion and dental extractions. Materials and Methods: The sample consisted of 60 dental casts taken before (n = 30) and after (n = 30) orthodontic treatment from patients aged >18 years. The patients were treated with rapid maxillary expansion by using Haas appliance (n = 10), by extracting the maxillary first premolars (n = 10) and by using only conventional fixed orthodontic appliances (n = 10). All the dental casts were analyzed twice by two independent examiners that were blind for the type of treatment. Results: All the differences between groups were statistically significant (P < 0.05). More alterations in the morphology of the palatal rugae were observed in patients treated with rapid maxillary expansion, whereas few alterations were performed in patients treated with no maxillary expansion or dental extractions. Conclusion: The use of palatal rugae for forensic purposes must be avoided in patients that underwent invasive orthodontic treatments, such as those founded on maxillary expansion and dental extractions.


Assuntos
Técnica de Expansão Palatina , Palato , Adolescente , Dente Pré-Molar , Humanos , Mucosa Bucal , Extração Dentária
9.
Stomatologiia (Mosk) ; 99(2): 55-60, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32441077

RESUMO

The aim of the study was to assess the state of the mucous membrane of the prosthetic bed, the features of fixation of removable dentures taking into account the sex and age of the patients, as well as the determination of the feasibility of using dentures fixing agents. MATERIALS AND METHODS: The clinical and sociological study was conducted involving 283 (130 men and 153 women) patients aged from 45 to 92 years. All patients had acrylic removable dentures on one or both jaws. If patients had complaints about insufficient fixation of the denture, they were recommended to use a prosthesis fixing cream for daily use. After 14 days, patients were examined again for the dynamics of complaints, as well as changes in the mucous membrane of the prosthetic bed. RESULTS: Regardless of gender and age group patients using partial and complete dentures rarely (in 4.3% of cases) used dentures fixing agents, although 40.8% had poor fixation and (or) stabilization of removable acrylic partial and full dentures, and in 51.9% of cases inflammatory changes of the mucous membrane of the prosthetic bed were detected. Correction of removable dentures, as well as daily use of a denture fixation cream, made it possible within two weeks to reduce by 72.5% of patients complaints about poor fixation and (or) stabilization of removable dentures, as well as to eliminate existing inflammatory changes in 86.4% of patients.


Assuntos
Prótese Parcial Removível , Mucosa Bucal , Idoso , Idoso de 80 Anos ou mais , Prótese Total , Prótese Parcial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
10.
Stomatologiia (Mosk) ; 99(2): 110-113, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32441085

RESUMO

This article provides the current data of the modern literature on the question of the biocompatibility of dental materials, their influence on the local immune homeostasis of the oral cavity; influence of the method of transmission of masticatory load on the tissues and organs of the oral cavity. Thus, when installing the seal, the choice of material, the localization of carious lesions, and when prosthetics - the choice of material of the prosthetic structure and the method of transfer of chewing load is important. It should be understood that the reaction of the mucous membrane can be not only in the area of direct contact with the dental material, but also appear in remote areas of the oral mucosa.


Assuntos
Cárie Dentária , Materiais Dentários , Humanos , Mastigação , Mucosa Bucal
12.
J Contemp Dent Pract ; 21(1): 83-90, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32381807

RESUMO

AIM: To study the efficacy of topical curcumin mucoadhesive semisolid gel, triamcinolone acetonide/hyaluronidase mucoadhesive semisolid gel, and a combination of both in the treatment of oral submucous fibrosis (OSMF). MATERIALS AND METHODS: One hundred and twenty patients diagnosed with OSMF were randomly divided into groups I, II, and III. Each patients in groups I, II, and III was given professionally prepared mucoadhesive semisolid gel of curcumin, a combination of triamcinolone acetonide and hyaluronidase mucoadhesive semisolid gel, and a combination of all three, respectively. Patients were instructed to apply the gel thrice daily for 6 weeks on buccal mucosa bilaterally using the tip of index finger. Three parameters were evaluated at the end of each week, namely, mouth opening, burning on visual analog scale (VAS), and the color of oral mucosa on the binary scale. The results were subjected to statistical analysis. RESULTS: It was observed that the group administered the three drug combinations achieved the greatest mouth opening (mean increase 4.05 mm) as compared to the other two groups. It was observed that triamcinolone and hyaluronidase group reported reduction in burning sensation on VAS (mean difference 6) as compared to the other two groups. It was observed that group III (1% curcumin, 1% hyaluronidase and 0.1% triamcinolone acetonide combined) drug therapy showed better change in mucosa color as compared to groups I (1% curcumin) and II. CONCLUSION: Thus, we can conclude that curcumin has a therapeutic effect on patients diagnosed with OSMF. Maximum utilization and enhanced drug delivery were achieved with the help of a combination other two active drugs, namely, triamcinolone and hyaluronidase. CLINICAL SIGNIFICANCE: Curcumin role in the treatment of oral cancers and the precancer lesion is very promising.


Assuntos
Curcumina , Fibrose Oral Submucosa , Humanos , Hialuronoglucosaminidase , Mucosa Bucal , Triancinolona Acetonida
13.
Oral Oncol ; 105: 104741, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32380453

RESUMO

This article aims at collecting all information needed for dentists regarding the COVID-19 pandemic throughout the world by reviewing articles published by now. In late 2019, a pneumonia outbreak of uncertain etiology happened in Wuhan, China. There were many reports related to a live-animal and seafood market, supporting that the pathogens were transferred from animals to humans, rapidly evolving into transmission from human to human. The pathogen was classified as 2019 Novel Corona Virus (2019-nCoV), and the disease was named COrona VIrus Disease 2019 (COVID-19). Given that COVID-19 has lately been detected in infected patients' saliva, the COVID-19 outbreak is an alert that all dental and other health professionals must be vigilant in defending against the infectious disease spread, and it may enable to assess whether non-invasive saliva diagnostic for COVID-19. There has so far been no evidence from randomized controlled trials to prescribe any particular anti-nCoV treatment or vaccine, and COVID-19 management has been widely supportive. Since the ACE-2 was expressing on oral cavity mucosa, there is a potentially huge COVID-19 infectious vulnerability risk for oral cavity and brought up a proof for the future prevention procedure in dental practice and daily life. As a result, the whole dental teams should be vigilant and keep patients and themselves in a safe environment by following the guideline in this study.


Assuntos
Infecções por Coronavirus/epidemiologia , Odontólogos , Pneumonia Viral/epidemiologia , Betacoronavirus , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/transmissão , Mucosa Bucal/metabolismo , Mucosa Bucal/virologia , Pandemias , Peptidil Dipeptidase A/metabolismo , Pneumonia Viral/diagnóstico , Pneumonia Viral/transmissão , Guias de Prática Clínica como Assunto
14.
Compend Contin Educ Dent ; 41(5): e17-e22, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32369382

RESUMO

Fibromas often occur orally and are typically due to trauma to underlying connective tissue resulting in tissue enlargement. To prevent continuing trauma to the tissue, excision may be recommended, and biopsy is often indicated for these masses to confirm their diagnosis and to rule out a potential malignant cause. This article presents a case report in which a Er,Cr:YSSG laser was utilized for excision of an oral fibroma. This approach enabled excellent tissue response compared to the use of a scalpel or monopolar electrosurgery, with minimal patient postoperative discomfort and improved healing.


Assuntos
Fibroma , Terapia a Laser , Neoplasias Bucais , Humanos , Lasers , Mucosa Bucal
15.
Wiad Lek ; 73(5): 1037-1040, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32386391

RESUMO

OBJECTIVE: The aim: Analysis of literary sources with the study of modern views on the problem of the immune status of the oral mucosa. PATIENTS AND METHODS: Materials and methods: The review and analysis of scientific and medical literature for the years 2013-2019, based on Scopus databases, Web of Science, Med Line, The Cochrane Library, EMBASE, Global Health, CyberLeninka, RIC. CONCLUSION: Conclusions: Bacterial wall antigens are able to affect the local immune response of the oral mucosa. In particular, suppression of lymphocytes and macrophages proliferation, alteration of adhesion molecules expression and homing of the immune system cells to the inflammation site, inhibition of cytokine secretion by contact of the immune system cells with gram-positive bacteria is revealed. Therefore, studying the changes in the differentiation of the oral mucosa epithelium and periodontal tissues under the influence of environmental factors is of relevance. The conception of the oral cavity as a complex ecological system has not only theoretical but also practical value. Summarizing the data, we can say that at the current stage of science development the interaction of all structures should be considered, focusing on epitheliocytes of the oral mucosa, as a closing chain, which initiates changes that can affect the condition of the oral cavity and the results of pathological processes treatment of the oral cavity mucous membrane and periodontal tissues.


Assuntos
Mucosa Bucal , Boca , Células Epiteliais , Humanos , Inflamação
17.
Int J Mol Sci ; 21(10)2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32455539

RESUMO

The COVID-19 pandemic is marked by a wide range of clinical disease courses, ranging from asymptomatic to deadly. There have been many studies seeking to explore the correlations between COVID-19 clinical outcomes and various clinical variables, including age, sex, race, underlying medical problems, and social habits. In particular, the relationship between smoking and COVID-19 outcome is controversial, with multiple conflicting reports in the current literature. In this study, we aim to analyze how smoking may affect the SARS-CoV-2 infection rate. We analyzed sequencing data from lung and oral epithelial samples obtained from The Cancer Genome Atlas (TCGA). We found that the receptor and transmembrane protease necessary for SARS-CoV-2 entry into host cells, ACE2 and TMPRSS2, respectively, were upregulated in smoking samples from both lung and oral epithelial tissue. We then explored the mechanistic hypothesis that smoking may upregulate ACE2 expression through the upregulation of the androgen pathway. ACE2 and TMPRSS2 upregulation were both correlated to androgen pathway enrichment and the specific upregulation of central pathway regulatory genes. These data provide a potential model for the increased susceptibility of smoking patients to COVID-19 and encourage further exploration into the androgen and tobacco upregulation of ACE2 to understand the potential clinical ramifications.


Assuntos
Androgênios/metabolismo , Infecções por Coronavirus/metabolismo , Peptidil Dipeptidase A/genética , Pneumonia Viral/metabolismo , Serina Endopeptidases/genética , Fumar/metabolismo , Regulação para Cima , Células Epiteliais Alveolares/metabolismo , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/genética , Humanos , Mucosa Bucal/metabolismo , Pandemias , Peptidil Dipeptidase A/metabolismo , Pneumonia Viral/epidemiologia , Pneumonia Viral/genética , Receptores Androgênicos/genética , Receptores Androgênicos/metabolismo , Serina Endopeptidases/metabolismo , Fumar/epidemiologia , Fumar/genética
18.
PLoS One ; 15(5): e0231588, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32421698

RESUMO

We have recently established a protocol to grow wildtype human oral mucosa organoids. These three-dimensional structures can be maintained in culture long-term, do not require immortalization, and recapitulate the multilayered composition of the epithelial lining of the oral mucosa. Here, we validate the use of this model to study the effect of Leucovorin (LV) on Methotrexate (MTX)-induced toxicity. MTX is a chemotherapeutic agent used in the treatment of pediatric acute lymphoblastic leukemia. Although effective, the use of MTX often results in severe side-effects, including oral mucositis, which is characterized by epithelial cell death. Here, we show that organoids are sensitive to MTX, and that the addition of LV reduces MTX toxicity, in both a concentration- and timing-dependent manner. Additionally, we show that a 24 hour 'pretreatment' with LV reduces MTX-induced cell death, suggesting that such a pretreatment could decrease mucositis in patients. Taken together, we provide the first in vitro model to study the effect of MTX on wildtype oral mucosa cells. Our findings underscore the relevance of the clinically applied LV regimen and highlight the potential of this model to further optimize modifications in dosing and timing of Leucovorin on oral mucosa cells.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Apoptose/efeitos dos fármacos , Mucosa Bucal/efeitos dos fármacos , Organoides/patologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Estomatite/patologia , Adolescente , Criança , Humanos , Técnicas In Vitro , Leucovorina/administração & dosagem , Metotrexato/administração & dosagem , Mucosa Bucal/patologia , Técnicas de Cultura de Órgãos , Organoides/efeitos dos fármacos , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Estomatite/induzido quimicamente
19.
J Pediatric Infect Dis Soc ; 9(3): 370-372, 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32463086

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was detected from at least 1 buccal specimen in 9 of 11 coronavirus disease 2019 (COVID-19)-infected children (81.8%). Viral loads in buccal specimens were substantially lower than those in nasopharyngeal specimens. Buccal swabs are not good as COVID-19 screening specimens in children.


Assuntos
Betacoronavirus , Infecções por Coronavirus/diagnóstico , Mucosa Bucal/virologia , Pneumonia Viral/diagnóstico , Bochecha , Criança , Pré-Escolar , Infecções por Coronavirus/virologia , Humanos , Lactente , Nasofaringe/virologia , Pandemias , Pneumonia Viral/virologia , Reação em Cadeia da Polimerase em Tempo Real , Saliva/virologia , Carga Viral
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