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2.
Gene ; 736: 144447, 2020 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-32032744

RESUMO

BACKGROUND: Previous studies indicated that gastric acid secretion was associated with gastric cancer risk. However, it still needs to explore whether the related pathway genetic variation affects GC risk. METHODS: A bioinformatics study in 1625 gastric cancer cases and 2100 controls was conducted to investigate the relationship of genetic variants in eleven gastric acid secretion pathway genes and gastric cancer risk. We used logistic regression model with odds ratios (ORs) and 95% confidence intervals (CIs) to estimate the effect of 38 single nucleotide polymorphisms (SNPs) on gastric cancer susceptibility. Expression quantitative trait loci (eQTL) analysis and the methylation eQTL (meQTL) analysis were used to calculate the genetic effect on gene expression. RESULTS: We identified that the CpG-SNP rs11810577 in GNAI3 was significantly increased gastric cancer risk (OR = 1.19, 95% CI = 1.07-1.32, P = 1.12 × 10-3). Furthermore, rs11810577 T allele had a negative effect on adhesion molecule with Ig like domain 1 (AMIGO1) expression in gastric tissues and increased the methylation levels of cg18220030 and cg15694127 in the promoter region of AMIGO1. Moreover, we found AMIGO1 had a lower expression levels in gastric cancer tissues than normal tissues (P = 0.037), in agreement with the data results from The Cancer Genome Atlas (TCGA) database (P < 1.0 × 10-4). CONCLUSION: The CpG-SNP rs11810577 in GNAI3 in the gastric acid secretion pathway was significantly associated with susceptibility to gastric cancer.


Assuntos
Ilhas de CpG/genética , Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/genética , Ácido Gástrico/metabolismo , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Via Secretória/genética , Neoplasias Gástricas/genética , Alelos , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Glicoproteínas de Membrana/genética , Pessoa de Meia-Idade , Regiões Promotoras Genéticas/genética , Locos de Características Quantitativas/genética , Neoplasias Gástricas/metabolismo
3.
Microsc Res Tech ; 83(1): 3-9, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31603592

RESUMO

This study aimed to use scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS) to examine the elements that passed into the gastric acid solution after the application of a gastric acid erosive cycle to chitosan modified glass ionomer cement (GIC). Chitosan modified GIC samples were obtained by adding chitosan (vol/vol) of 5 and 10% to GIC for the experimental groups. These two experimental groups and a control group were subjected to gastric acid erosive treatment for 60 s six times a day for 10 days. The sample surfaces were coated with approximately 1 nm of gold to increase conductivity with the Q 150R ES device (Quorum Technologies, East Sussex, England). Surface topography images were obtained with a SEM. Besides, EDS analysis was also determined the number of elements graphically in the region where the fast electron beam hit. In the samples examined, the amount of element was determined. After gastric acid application, cracks and voids were observed on the surfaces of the samples. In the EDS analysis of the 5 and 10% chitosan modified GIC and control groups, Si, Al, Na, and F was found. It is necessary to investigate the antibacterial properties and physical properties of chitosan modified glass ionomer-free elements and fluorine ions using advanced techniques.


Assuntos
Quitosana/química , Ácido Gástrico/química , Cimentos de Ionômeros de Vidro/química , Animais , Microscopia Eletrônica de Varredura , Espectrometria por Raios X , Suínos
4.
J Prosthodont ; 29(3): 207-218, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29333707

RESUMO

PURPOSE: To test the impact of exposure to artificial gastric acid combined with toothbrush abrasion on the properties of dental ceramics. Earlier research has indicated that immersion in artificial gastric acid has caused increased surface roughness of dental ceramics; however, the combined effects of acid immersion and toothbrush abrasion and the impact of increased surface roughness on mechanical strength and optical properties have not been studied. MATERIALS AND METHODS: Three commercially available ceramics were chosen for this study: feldspathic porcelain, lithium disilicate glass-ceramic, and monolithic zirconium oxide. The specimens (10 × 1 mm discs) were cut, thermally treated as required, and polished. Each material was divided into four groups (n = 8 per group): control (no exposure), acid only, brush only, acid + brush. The specimens were immersed in artificial gastric acid (50 ml of 0.2% [w/v] sodium chloride in 0.7% [v/v] hydrochloric acid mixed with 0.16 g of pepsin powder, pH = 2) for 2 minutes and rinsed with deionized water for 2 minutes. The procedure was repeated 6 times/day × 9 days, and specimens were stored in deionized water at 37°C. Toothbrush abrasion was performed using an ISO/ADA design brushing machine for 100 cycles/day × 9 days. The acid + brush group received both treatments. Specimens were examined under SEM and an optical microscope for morphological changes. Color and translucency were measured using spectrophotometer CIELAB coordinates (L*, a*, b*). Surface gloss was measured using a gloss meter. Surface roughness was measured using a stylus profilometer. Biaxial flexural strength was measured using a mechanical testing machine. The data were analyzed by one-way ANOVA followed by Tukey's HSD post hoc test (p < 0.05). RESULTS: Statistically significant changes were found for color, gloss, and surface roughness for porcelain and e.max specimens. No statistically significant changes were found for any properties of zirconia specimens. CONCLUSIONS: The acid treatment affected the surface roughness, color, and gloss of porcelain and e.max ceramics. The changes in translucency and mechanical strength for all materials were not statistically significant. Zirconia ceramic showed resistance to all treatments.


Assuntos
Materiais Dentários , Ácido Gástrico , Cerâmica , Porcelana Dentária , Teste de Materiais , Propriedades de Superfície , Zircônio
5.
Dig Dis ; 38(2): 104-111, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31846972

RESUMO

Less than 2 centuries have elapsed since the identification of hydrochloric acid in the stomach. The clarification of the molecular mechanisms allowed the effective therapeutic suppression of gastric acid secretion. The spectacular advances in the treatment of acid-related disorders represent a synthesis of the contributions of several brilliant pharmacologists, basic scientists, and clinical physicians. Effective gastric acid suppressive therapy has dramatically improved the therapy and outcome of acid-related disorders. The introduction of proton pump inhibitors (PPIs) in clinical practice has significantly changed the medical management of upper gastrointestinal disorders. PPIs represent the "gold-standard" therapy in acid-related disorders. However, some challenges persist in the therapy of acid related diseases, including management of patients who respond inadequately to PPI therapy, more effective gastroprotection, or more powerful antisecretory treatment for the eradication of Helicobacter pylori infection. New antisecretory drugs are currently being developed and investigated to further provide a more effective and profound gastric acid secretion inhibition. The major advance has been the development of acid pump -antagonists, the potassium channel acid blocking drugs (-P-CABs). Long-term studies comparing P-CABs with PPIs will help to define the exact place and safety profile of this class of drug in the management of acid-related disorders.


Assuntos
Ácido Gástrico/metabolismo , Fármacos Gastrointestinais/farmacologia , Desenvolvimento de Medicamentos , Fármacos Gastrointestinais/uso terapêutico , Antagonistas dos Receptores Histamínicos/farmacologia , Humanos , Bloqueadores dos Canais de Potássio/farmacologia , Inibidores da Bomba de Prótons/efeitos adversos , Inibidores da Bomba de Prótons/uso terapêutico
6.
Int J Mol Sci ; 20(23)2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31795477

RESUMO

Gastric juice is a unique combination of hydrochloric acid (HCl), lipase, and pepsin. Acidic gastric juice is found in all vertebrates, and its main function is to inactivate microorganisms. The phylogenetic preservation of this energy-consuming and, at times, hazardous function (acid-related diseases) reflects its biological importance. Proton pump inhibitors (PPIs) are one of the most widely used drugs in the world. Due to the reduced prevalence of Helicobacter pylori infection as well as the increased use of inhibitors of gastric acid secretion, the latter has become the most important cause of gastric hypoacidity. In the present manuscript, we review the microbiological consequences of removing gastric acidity. The resulting susceptibility to infections has not been studied extensively, and focus has mainly been restricted to bacterial and parasitic agents only. The strongest evidence concerning the relationship between hypochlorhydria and predisposition to infections relates to bacterial infections affecting the gastrointestinal tract. However, several other clinical settings with increased susceptibility to infections due to inhibited gastric acidity are discussed. We also discuss the impact of hypochlorhydria on the gut microbiome.


Assuntos
Acloridria/induzido quimicamente , Ácido Gástrico/metabolismo , Suco Gástrico/efeitos dos fármacos , Suco Gástrico/microbiologia , Inibidores da Bomba de Prótons/efeitos adversos , Acloridria/complicações , Acloridria/metabolismo , Animais , Infecções Bacterianas/etiologia , Suco Gástrico/metabolismo , Gastroenteropatias/etiologia , Microbioma Gastrointestinal/efeitos dos fármacos , Humanos , Infecções/etiologia , Inibidores da Bomba de Prótons/farmacologia
8.
Gastroenterol Clin North Am ; 48(4): 465-470, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31668176

RESUMO

A total parenteral nutrition (TPN) formula needs to be correctly compounded with the help of a pharmacist and patients cycled to ensure they are tolerating the TPN volume. Selection of and close working relationship with a home infusion company needs to be arranged prior to hospital discharge and can be coordinated with the help of a hospital case manager. For Medicare patients, a certificate of medical necessity must be completed and signed prior to hospital discharge. Patients should undergo education regarding catheter care, infusion pump programming, and preparation of the TPN solution with additives, such as multivitamins and trace elements.


Assuntos
Complicações Pós-Operatórias/terapia , Síndrome do Intestino Curto/terapia , Antidiarreicos/uso terapêutico , Diarreia/prevenção & controle , Eletrólitos/administração & dosagem , Hidratação , Ácido Gástrico/metabolismo , Humanos , Nutrição Parenteral , Soluções de Nutrição Parenteral , Cuidados Pós-Operatórios , Oligoelementos/administração & dosagem , Vitaminas/administração & dosagem
9.
Prague Med Rep ; 120(2-3): 52-63, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31586504

RESUMO

Tyrosine kinase inhibitors have recently become an essential tool in management of chronic myeloid leukaemia (CML). Dasatinib, a representative of those drugs, acts by inhibiting key proteins included in CML development, predominantly Bcr-Abl and Src. Its advantage is that it shows activity in many cases where other agents bring no improvement due to resistance. Pharmacokinetics of dasatinib has specific characteristics that may play an important role in achieving sufficient exposure in patients. Therefore, the key pharmacokinetic properties are summarized in this report. For example, dasatinib absorption is significantly influenced by gastric pH and its modulation can be a source of serious interactions, as well as simultaneous administration of drugs affecting cytochrome P450.


Assuntos
Dasatinibe/farmacocinética , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Sistema Enzimático do Citocromo P-450 , Dasatinibe/uso terapêutico , Resistencia a Medicamentos Antineoplásicos , Ácido Gástrico , Humanos , Concentração de Íons de Hidrogênio , Leucemia Mielogênica Crônica BCR-ABL Positiva/metabolismo , Inibidores de Proteínas Quinases/farmacocinética , Inibidores de Proteínas Quinases/uso terapêutico
10.
Pharm Res ; 36(11): 155, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31485804

RESUMO

PURPOSE: The purpose of this study was to determine the impact of food on gastric pH and the ability of over the counter betaine hydrochloride (BHCl) acid to reacidify gastric pH after food-induced elevations in gastric pH. METHODS: This open-label cross over clinical study (NCT02758015) included 9 subjects who were randomly assigned to one of 16 possible, 4-period cross-over sequences to determine the impact and relationship of food and gastric pH with acid supplementation. Subjects were administered various doses (1500 mg, 3000 mg and 4500 mg) of betaine hydrochloride (BHCl) to determine the ability of acid supplementation to reacidify gastric pH after the elevation of gastric pH caused by the ingestion of food. RESULTS: Following the administration of food and the resulting elevation in gastric pH, time to return to baseline gastric pH levels without acid supplementation was 49.7 ± 14.0 min. Administering 4500 mg of BHCl acid in capsules was able to reacidify gastric pH levels back to baseline following the administration of food in approximately 17.3 ± 5.9 min. AUCpH of each treatment were similar and not statistically different. Mean max pH following the administration of food was 3.20 ± 0.55. CONCLUSION: The ability of food to elevate and maintain gastric pH levels in the presence of acid supplementation was made evident throughout the study. A 4500 mg dose of BHCl was required to reacidify gastric pH after the administration of food. This study details the difficulty faced by clinicians in dosing a poorly soluble, weakly basic drug to patients receiving acid reducing agents where administration with food is recommended to avoid gastric side effects. TRIAL REGISTRATION: https://clinicaltrials.gov/ct2/show/NCT02758015.


Assuntos
Betaína/uso terapêutico , Alimentos , Absorção Gástrica , Ácido Gástrico/metabolismo , Fármacos Gastrointestinais/uso terapêutico , Preparações Farmacêuticas/metabolismo , Administração Oral , Adolescente , Adulto , Estudos Cross-Over , Feminino , Interações Alimento-Droga , Determinação da Acidez Gástrica , Humanos , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
11.
J Environ Sci (China) ; 85: 200-207, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31471027

RESUMO

The exposure to plastic debris and associated pollutants for wildlife is of urgent concern, but little attention has been paid on the transfer of plastic additives from plastic debris to organisms. In the present study, the leaching of incorporated flame retardants (FRs), including polybrominated diphenyl ethers (PBDEs), alternative brominated FRs (AFRs), and phosphate flame retardants (PFRs), from different sizes of recycled acrylonitrile-butadiene-styrene (ABS) polymer were investigated in avian digestive fluids. The impact of co-ingested sediment on the leaching of additive-derived FRs in digestive fluids was also explored. In the recycled ABS, BDE 209 (715 µg/g) and 1, 2-bis(2,4,6-tribromophenoxy) ethane (BTBPE, 1766 µg/g) had the highest concentrations among all target FRs. The leaching proportions of FRs were higher in finer sizes of ABS. The leaching proportions of FRs from recycled ABS increased with elevated logKOW of FRs. In the tests with coexisted ABS and sediment, hexa- to deca-BDEs, BTBPE, and decabromodiphenyl ethane (DBDPE) migrated from ABS to sediment, which resulted in the less bioaccessible fractions of these FRs in gut fluids. More lipophilic chemicals tended to be adsorbed by sediment from ABS. The results suggest the migration of additive-derived FRs from plastics to other indigestible materials in digestive fluids. The findings in this study provide insights into the transfer of additive-derived FRs from plastics to birds, and indicate the significant contribution of FR-incorporated plastics to bioaccumulation of highly lipophilic FRs.


Assuntos
Aves , Poluentes Ambientais/química , Retardadores de Chama/análise , Ácido Gástrico/química , Plásticos/química , Animais , Bromobenzenos/química , Butadienos , Exposição Ambiental , Éteres Difenil Halogenados/química , Organofosfatos
13.
Georgian Med News ; (291): 7-13, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31418722

RESUMO

The purpose of the study was to identify the pathogenesis features of prepiloric and pyloric ulcers and to establish the cause of resistance to conservative therapy and the tendency to complicated course of type III ulcers according to H.D. Johnson. A comparative analysis of acid production was carried out in 150 patents with duodenal ulcer (n-80) and peptic ulcer of type III according to H.D. Johnson classification (n-70). We revealed a definite individual rhythm of daily intragastric acidity with a maximum rise at night in patients with duodenal ulcer. The paper explores the peculiarities of digestive juice secretion depending on ulcerative substratum localization. The pathogenetic validity of the use of antisecretory drugs for duodenal ulcer treatment and the need to further improve the algorithm for pyloric and prepyloric ulcer therapy are shown. For pyloric and prepiloric ulcers characteristic specific morphological changes in the muscle layer of the wall of the antrum, leading to the motor-evacuation disorders and promoting duodenogastric reflux - dystrophy myocytes circular muscle layer vacuolation of the cytoplasm, edema and hypertrophy ganglia intermuscular plexus, hemorrhages in the muscle sheath, violation of muscle bundles.


Assuntos
Úlcera Duodenal/complicações , Úlcera Duodenal/patologia , Ácido Gástrico/metabolismo , Piloro/patologia , Úlcera Gástrica/complicações , Úlcera Gástrica/patologia , Úlcera Duodenal/metabolismo , Humanos , Úlcera Gástrica/metabolismo
14.
Med Sci Monit ; 25: 6153-6164, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31419218

RESUMO

BACKGROUND The clinical association between gastroesophageal reflux disease (GERD) and idiopathic pulmonary fibrosis (IPF) has been known for many years, but it is still unclear. The present study investigated the association between experimentally simulated aspiration and pulmonary fibrosis. MATERIAL AND METHODS A total of 120 male Sprague-Dawley rats were randomly divided into a negative control group, a bleomycin group, and 3 simulated aspiration groups. The bleomycin group was administered a one-time intratracheal injection of bleomycin, whereas the 3 simulated aspiration groups were treated either with an intratracheal instillation of gastric fluid combined with pepsin, with pepsin alone, or with hydrochloric acid, all twice a week, and the negative control group was administered normal saline twice a week. Lung tissues were collected to evaluate pathological changes and the mRNA expression levels of connective tissue growth factor (CTGF), type I collagen, and transforming growth factor. RESULTS The results demonstrated that the degree of fibrosis in the early stage was low in each of the 3 simulated aspiration groups, but gradually increased over time. The expression levels of the downstream factor of fibrosis, CTGF, and type I collagen also reflected this trend. CONCLUSIONS The study demonstrates that aspiration of gastric contents can cause pulmonary fibrosis, and mixed aspiration of pepsin and gastric fluid can accelerate this process. This study provides strong evidence in support of a potential association between human GERD and IPF.


Assuntos
Ácido Gástrico/metabolismo , Pepsina A/metabolismo , Fibrose Pulmonar/metabolismo , Administração por Inalação , Animais , Bleomicina/farmacologia , Proteína Rica em Cisteína 61/genética , Ácido Gástrico/fisiologia , Refluxo Gastroesofágico/complicações , Refluxo Gastroesofágico/fisiopatologia , Fibrose Pulmonar Idiopática/etiologia , Fibrose Pulmonar Idiopática/metabolismo , Pulmão/patologia , Masculino , Pepsina A/fisiologia , Fibrose Pulmonar/etiologia , Fibrose Pulmonar/fisiopatologia , Ratos , Ratos Sprague-Dawley , Fatores de Crescimento Transformadores/genética
15.
Nat Commun ; 10(1): 3298, 2019 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-31363098

RESUMO

Gastric acid suppression promotes allergy in mechanistic animal experiments and observational human studies, but whether gastric acid inhibitors increase allergy incidence at a population level remains uncharacterized. Here we aim to assess the use of anti-allergic medication following prescription of gastric acid inhibitors. We analyze data from health insurance records covering 97% of Austrian population between 2009 and 2013 on prescriptions of gastric acid inhibitors, anti-allergic drugs, or other commonly prescribed (lipid-modifying and antihypertensive) drugs as controls. Here we show that rate ratios for anti-allergic following gastric acid-inhibiting drug prescriptions are 1.96 (95%CI:1.95-1.97) and 3.07 (95%-CI:2.89-3.27) in an overall and regional Austrian dataset. These findings are more prominent in women and occur for all assessed gastric acid-inhibiting substances. Rate ratios increase from 1.47 (95%CI:1.45-1.49) in subjects <20 years, to 5.20 (95%-CI:5.15-5.25) in > 60 year olds. We report an epidemiologic relationship between gastric acid-suppression and development of allergic symptoms.


Assuntos
Antiulcerosos/efeitos adversos , Hipersensibilidade/epidemiologia , Inibidores da Bomba de Prótons/efeitos adversos , Adolescente , Adulto , Idoso , Antiulcerosos/uso terapêutico , Áustria/epidemiologia , Feminino , Ácido Gástrico/química , Humanos , Hipersensibilidade/etiologia , Hipersensibilidade/imunologia , Sistema Imunitário/efeitos dos fármacos , Sistema Imunitário/imunologia , Incidência , Masculino , Registros Médicos/estatística & dados numéricos , Pessoa de Meia-Idade , Inibidores da Bomba de Prótons/uso terapêutico , Adulto Jovem
16.
Aliment Pharmacol Ther ; 50(7): 751-759, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31437865

RESUMO

BACKGROUND: Tegoprazan (CJ-12420) is a potassium-competitive acid blocker (P-CAB) with therapeutic potential for gastro-oesophageal reflux disease (GERD) by reversibly suppressing gastric H+ /K+ -ATPase. AIMS: To investigate the safety, tolerability, pharmacokinetics and pharmacodynamics of tegoprazan METHODS: A phase I, randomised, double-blind and placebo-controlled clinical trial was conducted in 56 healthy male subjects without Helicobacter pylori infection. In the single ascending dose study, 50, 100, 200 and 400 mg tegoprazan were administered to 32 subjects. In the multiple ascending dose study, 100 and 200 mg tegoprazan were administered every 24 hours to each of the eight subjects for 7 days. In the comparative pharmacodynamics study, 40 mg esomeprazole was administered to eight subjects every 24 hours for 7 days. The assessment included safety, tolerability, pharmacodynamics through monitoring of 24-hour gastric pH and pharmacokinetics of tegoprazan in plasma and urine. RESULTS: Tegoprazan was generally well tolerated. Most adverse events reported in the study were mild in intensity and resolved without any sequelae. Exposure to tegoprazan increased in a dose-proportional manner. Multiple dosing with tegoprazan showed no accumulation in plasma on day 7. The pharmacodynamic analysis revealed that tegoprazan showed rapid, dose-dependent gastric acid suppression. CONCLUSIONS: Tegoprazan was well tolerated and showed rapid and potent gastric acid suppression. This supports the further development of tegoprazan as a treatment for acid-related disorders.


Assuntos
Derivados de Benzeno/administração & dosagem , Ácido Gástrico/metabolismo , Imidazóis/administração & dosagem , Administração Oral , Adulto , Relação Dose-Resposta a Droga , Método Duplo-Cego , Esomeprazol/administração & dosagem , ATPase Trocadora de Hidrogênio-Potássio/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
17.
J Appl Microbiol ; 127(5): 1564-1575, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31330563

RESUMO

AIMS: First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using ultraviolet-C light (UV-C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate the subsequent survival and persistence of cells in simulated gastric fluid (SGF). METHODS AND RESULTS: Two strains of E. coli O26, a wild-type strain and a clinical ATCC strain were used in both steps. A CCRD was used in a 22 arrangement in random order. The goodness-of-fit of the models was determined. The lack of fit, and the normality of residual data were checked with the Shapiro-Wilk test, and the model accuracy factor, bias factor and the model mean square error (MSE) were measured. Subsequently, the resistance capacity of the strains was evaluated after exposure to simulated gastric acid. The CCRD results obtained indicate that the mild heat (<70°C) has a recovery effect. In addition, for the clinical strain, the UV-C and heat (above 70°C) has an additive inactivation effect. Moreover, temperature (65°C) induced SGF resistance by the wild-type and clinical strain. For the clinical strain, cells exposed to UV-C were more sensitive to SGF. In contrast to clinical strain, exposing cells of the wild-type strain to UV-C increased the survival capacity in the SGF. CONCLUSION: Response surface analyses showed that the wild-type O26 strain has higher persistence under unfavourable conditions than the clinical strain, and the stresses caused by applied microbial control technologies can increase the survival capacity in the SGF. SIGNIFICANCE AND IMPACT OF THE STUDY: The present study shed light on different phenotypic responses in the same bacterium serogroup. Moreover, the impact of the study was that strain selection criteria must be adequate to develop effective models of inactivation.


Assuntos
Escherichia coli/efeitos da radiação , Ácido Gástrico/química , Contagem de Colônia Microbiana , Escherichia coli/química , Escherichia coli/crescimento & desenvolvimento , Infecções por Escherichia coli/microbiologia , Temperatura Alta , Humanos , Temperatura , Raios Ultravioleta
18.
Food Chem ; 298: 125042, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31261006

RESUMO

The bioavailability of the pyridoxal (PL), pyridoxine (PN), and pyridoxamine (PM) forms of vitamin B6 is different, considering that their bioaccessibility in baby foods is important for infant and young children's nutrition. The aim of this study was to determine and evaluate the bioaccessibility of the PL, PN, and PM forms of vitamin B6 in cereal-based baby foods an in vitro digestive system. In this study, the PL, PN, and PM forms of vitamin B6 were determined using HPLC in 13 cereal-based baby foods. The average bioaccessibility of the PL, PN, and PM forms in gastric pH 1.5 were 53%, 76%, and 50%, respectively. When the gastric pH was 4, the average bioaccessibility of PL, PN, and PM were 38%, 67%, and 36%, respectively. As observed in this study, the bioaccessibility of the PL, PN, and PM forms of vitamin B6 in baby foods is lower in both gastric pHs.


Assuntos
Alimentos Infantis/análise , Piridoxal/química , Piridoxamina/química , Piridoxina/química , Vitamina B 6/química , Grão Comestível/química , Ácido Gástrico/química , Humanos , Concentração de Íons de Hidrogênio , Lactente , Vitamina B 6/análise
19.
Nutrients ; 11(7)2019 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-31261930

RESUMO

We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis.


Assuntos
Brassica , Suplementos Nutricionais , Glucosinolatos/administração & dosagem , Glicosídeo Hidrolases/administração & dosagem , Imidoésteres/administração & dosagem , Isotiocianatos/metabolismo , Omeprazol/administração & dosagem , Extratos Vegetais/administração & dosagem , Inibidores da Bomba de Prótons/administração & dosagem , Plântula , Sementes , Adulto , Idoso , Disponibilidade Biológica , Brassica/química , Suplementos Nutricionais/efeitos adversos , Interações Medicamentosas , Feminino , Ácido Gástrico/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Glucosinolatos/efeitos adversos , Glucosinolatos/isolamento & purificação , Glucosinolatos/metabolismo , Glicosídeo Hidrolases/efeitos adversos , Glicosídeo Hidrolases/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Imidoésteres/efeitos adversos , Imidoésteres/isolamento & purificação , Imidoésteres/metabolismo , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Masculino , Pessoa de Meia-Idade , Omeprazol/efeitos adversos , Projetos Piloto , Extratos Vegetais/efeitos adversos , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/metabolismo , Inibidores da Bomba de Prótons/efeitos adversos , Plântula/química , Sementes/química , Adulto Jovem
20.
Gan To Kagaku Ryoho ; 46(7): 1159-1163, 2019 Jul.
Artigo em Japonês | MEDLINE | ID: mdl-31296822

RESUMO

Erlotinibis known as a key drug for the treatment of non-small-cell lung cancer. It is known to interact with gastric acid suppressing medications(AS). Concurrent administration of erlotinibwith AS is reported to decrease AUC and Cmax of erlotinib. From the result of a survey on concurrent administration of erlotinib with AS in our hospital, we considered that intake of erlotinib between meals in the morning and intake of AS after dinner or at bedtime certainly reduce the chance of drug interactions to a minimum. We suggested a direction of use of erlotinibto the doctors who used to prescribe this medication in their daily medical practice. We surveyed the doctors' perceptions of drug interactions. The results of the survey showed that 29% of the doctors were not concerned about drug interactions, although 81% of the doctors approved our proposal. By providing a suggestion for drug administration by analyzing drug information, the expectations of the doctors can be met and it also demonstrates the efficiency of pharmacists.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Cloridrato de Erlotinib/efeitos adversos , Neoplasias Pulmonares , Interações Medicamentosas , Ácido Gástrico , Humanos , Inquéritos e Questionários
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