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1.
West Afr J Med ; 37(1): 85-87, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32030717

RESUMO

BACKGROUND AND OBJECTIVES: Paragonimiasis is endemic in Eastern Nigeria. An upsurge was recorded after the Nigeria/Biafra war as protein lack in Biafra forced people to eat fresh water crabs. Its protean manifestations create confusion with several diseases. Elimination was assumed after a while and suspicion index fell. The interest in reporting this case follows its presentation outside the traditional endemic zone. RESULTS: The patient, though living in Eastern Nigeria and manifesting several pointers of Paragonimiasis, was treated as tuberculosis despite negative sputum AFB; without improving. He then presented up-country in Jos where history led to suspicion and confirmation of Paragonimiasis. By this time he had severe cor-pulmonale and died despite treatment. CONCLUSION: In this current economic downturn in Nigeria which may drive people to cheaper protein sources, a high index of suspicion should be raised for paragonimiasis when a patient presents with chronic cough productive of AFB-negative sputum and haemoptysis.


Assuntos
Paragonimíase/diagnóstico , Paragonimus/isolamento & purificação , Animais , Diagnóstico Diferencial , Evolução Fatal , Humanos , Pneumopatias Parasitárias , Masculino , Nigéria , Paragonimíase/parasitologia , Paragonimus/classificação , Escarro/parasitologia , Tuberculose Pulmonar/diagnóstico
3.
Acta Trop ; 199: 105074, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31295431

RESUMO

Paragonimiasis, or lung fluke disease, is a typical food-borne parasitic zoonosis caused by infection with trematodes belonging to the genus Paragonimus. More than 50 species of Paragonimus have been reported throughout the world, of which seven valid species infect humans, an estimated one million people annually worldwide. Among the seven species, P. westermani, P. heterotremus, and P. skrjabini/P. s. miyazakii, distributed in Asia, are the most important species as the cause of paragonimiasis. Humans acquire infection through the ingestion of raw, pickled or undercooked freshwater crustaceans, 2nd intermediate hosts, or consuming raw meat of wild boar or deer, paratenic hosts. Infections often occur clustered in foci where dietary habits allow transmission of the parasites. Paragonimiasis typically causes a subacute to chronic inflammatory disease of the lungs. The symptoms, including chronic cough, chest pain, dyspnea and hemoptysis, mimic those of tuberculosis and lung cancer. Serologic tests are commonly used for the diagnosis of paragonimiasis, and Praziquantel is the treatment of choice. In this review, the current status of Paragonimus and paragonimiasis in Asia is outlined based on the latest information and findings. We also summarize current trends of paragonimiasis in Japan, which is one of the most endemic area of paragonimiasis in the world, for the better understanding and control of paragonimiasis.


Assuntos
Paragonimíase/tratamento farmacológico , Paragonimíase/epidemiologia , Paragonimus/isolamento & purificação , Animais , Ásia/epidemiologia , Vetores de Doenças , Humanos , Paragonimus/classificação , Praziquantel/uso terapêutico
4.
Adv Exp Med Biol ; 1154: 105-138, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31297761

RESUMO

Paragonimiasis is a zoonotic disease caused by lung flukes of the genus Paragonimus. Humans usually become infected by eating freshwater crabs or crayfish containing encysted metacercariae of these worms. However, an alternative route of infection exists: ingestion of raw meat from a mammalian paratenic host. Adult worms normally occur in pairs in cysts in the lungs from which they void their eggs via air passages. The pulmonary form is typical in cases of human infection due to P. westermani, P. heterotremus, and a few other species (Table 5.1). Worms may occupy other sites in the body, notably the brain, but lung flukes have made their presence felt in almost every organ. Ectopic paragonimiasis is particularly common when infection is due to members of the P. skrjabini complex (Table 5.1). Human paragonimiasis occurs primarily in the tropics and subtropics of Asia, Africa, and the Americas, with different species being responsible in different areas (Table 5.1).


Assuntos
Paragonimíase , Zoonoses , África , América , Animais , Ásia , Braquiúros/parasitologia , Humanos , Paragonimíase/parasitologia , Paragonimíase/transmissão , Paragonimus , Clima Tropical , Zoonoses/parasitologia , Zoonoses/transmissão
5.
J Vet Med Sci ; 81(8): 1109-1112, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31189784

RESUMO

Paragonimiasis is an important food-borne zoonosis caused by Paragonimus flukes and is endemic to western Japan. However, there have been few epidemiological studies in the Tohoku district of northeastern Japan. In this study, Paragonimus metacercariae (mc) was detected in Geothelphusa dehaani (Japanese freshwater crab or Sawagani) in Iwate Prefecture. Out of the 207 Sawagani collected from 35 localities, 12 individuals from six localities were infected with Paragonimus mc. The mc were identified as P. skrjabini miyazakii based on the sequences of nuclear ribosomal internal transcribed spacer 2 and mitochondrial cytochrome c oxidase I. This is the first report of P. s. miyazakii mc infection in Sawagani in Iwate Prefecture.


Assuntos
Braquiúros/parasitologia , Paragonimíase/veterinária , Paragonimus/isolamento & purificação , Animais , Japão , Metacercárias/isolamento & purificação , Tipagem Molecular/veterinária , Paragonimíase/parasitologia
6.
Int J Infect Dis ; 83: 102-108, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30930185

RESUMO

OBJECTIVES: The pericardial effusion (PE) caused by paragonimiasis is rarely reported. This study aims to present experience in the diagnosis and surgical management of PE due to paragonimiasis. METHODS: Medical records of 57 children who were diagnosed with PE due to paragonimiasis and underwent surgery at Children's Hospital of Chongqing Medical University between January 2012 and August 2018 were retrospectively reviewed. RESULTS: The average age of this group was 7.6 ± 3.0 years. Patients were mainly from Chongqing and Sichuan areas. ELISA for Paragonimus skrjabini in all 57 patients showed positive results. Moderate or large PE were identified in 12 and 45 patients, respectively. All patients underwent surgery either by pericardectomy or thoracoscopic surgery. Pathological exams indicated massive eosinophil infiltration in all 57 specimens. After 3-4 courses of praziquantel therapy, the clinical outcomes were satisfactory. CONCLUSIONS: Typical endemic history, eosinophilia and multiple serous effusion raise suspicions of paragonimiasis. Once moderate to large PE is identified in patients with paragonimiasis, surgical treatment is necessary.


Assuntos
Paragonimíase/complicações , Derrame Pericárdico/diagnóstico , Derrame Pericárdico/cirurgia , Adolescente , Animais , Anti-Helmínticos/uso terapêutico , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Eosinofilia/parasitologia , Feminino , Humanos , Masculino , Paragonimus , Derrame Pericárdico/parasitologia , Praziquantel/uso terapêutico , Estudos Retrospectivos
8.
Infect Dis Poverty ; 7(1): 110, 2018 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-30342548

RESUMO

BACKGROUND: Paragonimiasis, caused by helminths of the genus Paragonimus spp., is a neglected tropical disease. Human suffering from paragonimiasis is often misunderstood and its quantification by the disability weight of the disability-adjusted life years largely varies in different global burden of disease (GBD) estimates. This paper is to systematically review clinical paragonimiasis cases and requantify the disability weight of human paragonimiasis. METHODS: A systematic analysis was conducted using articles from the following databases: PubMed, Institute for Scientific Information Web of Science, China National Knowledge Infrastructure, the Chinese scientific journal databases Wanfang Data and CQVIP, Africa Journal Online, and the System for Information on Grey Literature in Europe. Search terms were the combination of "paragonim*" with "clinical" or "infection". Only articles fulfilling the following conditions were recruited for this study: the occurrence of clinical signs and symptoms of paragonimiasis in human beings were reported; diagnosis was confirmed; no comorbidities were reported; the reviewed clinical cases or epidemiological findings were not already included in any other articles. The information and frequencies of paragonimiasis outcomes from included articles using predefined data fields were extracted two times by two separate individuals. Outcome disability weights were selected mainly from the GBD 2004 and GBD 2013 datasets. Frequencies and disability weights of paragonimiasis outcomes were modelled into a decision tree using the additive approach and multiplicative approach, respectively. Monte Carlo simulations were run 5000 times for an uncertainty analysis. RESULTS: The disability weight estimates of paragonimiasis were simulated with 5302 clinical cases from 80 general articles. The overall disability weight was estimated at 0.1927 (median 0.1956) with a 95% uncertainty interval (UI) of 0.1632-0.2378 using the additive approach, and 0.1791 (median 0.1816) with a 95% UI of 0.1530-0.2182 using the multiplicative approach. The simulated disability weights of Paragonimus westermani cases were higher than that of P. skrjabini cases. Lung outcomes and headache were the top two contributors to disability weight for both species. CONCLUSIONS: The use of paragonimiasis disability weight needs to be reconsidered with regard to availability of morbidity data and species variation. Calculating the disease burden of paragonimiasis requires further modification and thus has considerable implications for public health prioritization in research, monitoring, and control.


Assuntos
Pessoas com Deficiência/estatística & dados numéricos , Paragonimíase/epidemiologia , Animais , Efeitos Psicossociais da Doença , Tomada de Decisões , Árvores de Decisões , Humanos , Paragonimíase/parasitologia , Paragonimus , Avaliação de Resultados da Assistência ao Paciente
9.
Parasitology ; 145(13): 1748-1757, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30210013

RESUMO

Paragonimiasis, human lung fluke disease, is a foodborne anthropozoonosis caused by the trematodes assigned to Paragonimus and is regarded by the World Health Organization as a Neglected Tropical Disease (NTD). The life cycle of this medically important parasite centres on a complex freshwater biological community that includes two intermediate hosts: a mollusc and a decapod, usually a brachyuran. Although there is a perception that the biology, symptoms, diagnosis and treatment of Paragonimus is well understood, in reality, this is not the case, especially in Africa. Much remains unknown concerning the life-cycle of the parasite, its transmission, the current epidemiology of the disease, diagnosis and the effectiveness of treatment. Furthermore, cases of paragonimiasis may be misdiagnosed as resistant tuberculosis (TB) because of the similar pulmonary symptoms and no remission after anti TB therapy. The endemic foci of human paragonimiasis in Africa have been reported mainly in the forest zones of Upper Guinea (Liberia, Guinea and Ivory Coast) and Lower Guinea (Nigeria, Cameroon, Equatorial Guinea and Gabon). Despite the perceived medical importance of paragonimiasis, relatively little attention has been paid to this NTD since its discovery in Africa in the 1960s. This review focuses on the current understanding of the life cycle and transmission of Paragonimus in Africa, discusses its diagnosis and public health importance and highlights many outstanding gaps in the knowledge that still exist for this NTD.


Assuntos
Doenças Negligenciadas/epidemiologia , Paragonimíase/epidemiologia , Paragonimus/fisiologia , África/epidemiologia , África Central/epidemiologia , Animais , Braquiúros/parasitologia , Decápodes/parasitologia , Florestas , Humanos , Estágios do Ciclo de Vida , Doenças Negligenciadas/parasitologia , Saúde Pública , Zoonoses/diagnóstico , Zoonoses/parasitologia
10.
Parasit Vectors ; 11(1): 328, 2018 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-29848373

RESUMO

BACKGROUND: Members of the genus Paragonimus require at least three hosts in their life-cycles. The obligatory first intermediate hosts are freshwater snails. In Vietnam, although seven Paragonimus species have been recorded, the natural first intermediate hosts of almost all species have not been confirmed. The aim of this study was, therefore, to investigate snail hosts of Paragonimus species in Vietnam, and to identify Paragonimus species at intramolluscan stages. METHODS: Freshwater snails were collected from streams in Yen Bai and Quang Tri Provinces, where high prevalences of Paragonimus metacercariae in crab hosts have been reported. Snails were morphologically identified and then examined individually for Paragonimus cercariae using shedding and crushing methods. Chaetomicrocercous cercariae, the morphological class to which Paragonimus cercariae belong, were collected for morphological description and molecular species identification by analyses of ITS2 sequences. The infected snail species were identified based on analyses of nucleotide sequences of the cox1 gene. RESULTS: Three snail species were found to be infected with Paragonimus cercariae at low infection rates, ranging between 0.07-1.0%. The molecular analyses identified them as Sulcospira quangtriensis and 2 species of subfamily Triculinae. In a phylogenetic tree, these two triculine snails were related to the genera Gammatricula and Tricula with low posterior probabilities. Thus we named them as Triculinae sp. 1 and Triculinae sp. 2. Cercariae from the three snail species, Sulcospira quangtriensis, Triculinae sp. 1 and Triculinae sp. 2, were molecularly identified as Paragonimus westermani, P. heterotremus and P. proliferus, respectively. The cercariae of the three species are morphologically similar to each other, but their daughter rediae can be distinguished by the length of the intestine and the number of cercariae per redia. The rediae of P. westermani have a long intestine and each contain 6-8 cercariae. In contrast, those of P. heterotremus and P. proliferus have a short intestine and each redia contain 10-12 and 5-6 cercariae, respectively. CONCLUSIONS: Three snail species, Sulcospira quangtriensis, Triculinae sp. 1 and Triculinae sp. 2, serve as the first intermediate hosts of P. westermani, P. heterotremus and P. proliferus, respectively, in Vietnam. The length of the intestine of rediae and the number of cercariae per redia are valuable characteristics for distinguishing between larvae of these Paragonimus species.


Assuntos
Braquiúros/parasitologia , Paragonimíase/parasitologia , Paragonimus/classificação , Caramujos/parasitologia , Animais , Geografia , Especificidade de Hospedeiro , Larva , Estágios do Ciclo de Vida , Metacercárias , Paragonimus/genética , Paragonimus/isolamento & purificação , Filogenia , Caramujos/classificação , Vietnã/epidemiologia
11.
Korean J Parasitol ; 56(2): 189-194, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29742874

RESUMO

To determine that Paragonimus sp. is actively transmitted in a tropical area of the Pacific region of Ecuador where human cases of pulmonary paragonimiasis have recently been documented, a total of 75 freshwater crabs were collected from 2 different streams in the Pedernales area of Manabí Province, Ecuador. All collected crabs were identified as Hypolobocera guayaquilensis based on morphological characteristics of the male gonopods. The hepatopancreas of each crab was examined by compressing it between 2 glass plates followed by observation under a stereomicroscope. Excysted Paragonimus metacercariae were detected in 39 (52.0%) crabs and their densities varied from 1 to 32 per infected crab. There was a positive relationship between crab size and metacercarial density. Sequences of the second internal transcribed spacer region of the ribosomal RNA gene of the Paragonimus metacercariae obtained in this study were identical to those of Paragonimus mexicanus deposited in the DDBJ/EMBL/GenBank database. Thus, the present study is the first to confirm that the crab species H. guayaquilensis is the second intermediate host of P. mexicanus in Manabí Province, Ecuador. Because this crab might be the possible source of human infections in this area, residents should pay attention to improper crab-eating habits related with a neglected parasitic disease, i.e., paragonimiasis.


Assuntos
Decápodes/parasitologia , Doenças Transmitidas por Alimentos/parasitologia , Interações Hospedeiro-Parasita , Paragonimíase/parasitologia , Paragonimus/isolamento & purificação , Animais , Equador/epidemiologia , Feminino , Doenças Transmitidas por Alimentos/prevenção & controle , Humanos , Masculino , Metacercárias/isolamento & purificação , Paragonimíase/epidemiologia , Paragonimíase/prevenção & controle
12.
Parasit Vectors ; 11(1): 322, 2018 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-29843786

RESUMO

BACKGROUND: Paragonimus heterotremus is the main causative agent of paragonimiasis in Thailand. In Western blot diagnostic assays for paragonimiasis, the 35 kDa band present in crude P. heterotremus somatic extracts represents one of the known diagnostic bands. This study aimed to use a P. heterotremus cDNA library to create a recombinant version of this antigen for use in immunodiagnosis of paragonimiasis. METHODS: To accomplish this aim a cDNA expression library was constructed from adult worm mRNA and immuno-screened using antibodies from mice that had been immunized with the 35 kDa antigen. Screening resulted in the identification of an immunoreactive protein encoded by clone CE3, which contained an inserted sequence composed of 1292 base pairs. This clone was selected for use in the construction of a recombinant P. heterotremus protein because of its similarity to proactivator polypeptide. For recombinant protein expression, the CE3 gene sequence was inserted into the plasmid vector pRset and the resulting product had the expected molecular weight of 35 kDa. An IgG-ELISA based on the CE3 recombinant protein was evaluated by using sera from healthy individuals, from patients with paragonimiasis and other parasitic infections. This ELISA was performed by using human sera diluted at 1:2000, an optimized antigen concentration of 1 µg/ml, and anti-human IgG diluted at 1:4000. RESULTS: The cut-off optical density value was set as the mean + 2 standard deviations (0.54), which resulted in the test having a sensitivity of 88.89% and a specificity of 95.51%. The recombinant antigen could react with antibodies from P. heterotremus, P. pseudoheterotremus and P. westermani infections. Cross-reactivity occurred with a few cases of Blastocystis hominis infection (2/3), Bancroftian filariasis (1/10), opisthorchiasis (3/10), strongyloidiasis (4/10) and neurocysticercosis (1/11). CONCLUSIONS: Given the high test sensitivity and specificity, reflected in the low level of heterologous infection cross-reactivity (11/215 serum samples), observed in the IgG-ELISA, this 35 kDa antigen may be useful for the detection of paragonimiasis.


Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/imunologia , Paragonimíase/diagnóstico , Paragonimus/imunologia , Animais , Reações Cruzadas , Humanos , Imunoglobulina G/sangue , Paragonimíase/parasitologia , Proteínas Recombinantes , Sensibilidade e Especificidade , Testes Sorológicos
13.
Acta Trop ; 183: 95-102, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29596790

RESUMO

Paragonimiasis is a subacute to chronic inflammatory granulomatous lung disease caused by the genus Paragonimus. In Latin America Paragonimus mexicanus Miyazaki & Ishii, 1968 is the only confirmed species to cause human infections. Paragonimus caliensis Little, 1968 is an uncommon species often regarded as a synonym of P. mexicanus. Recently, the study of two types of Paragonimus metacercariae from Costa Rica has provided new molecular and morphological evidence that P. caliensis is a separate species from P. mexicanus. In the present study, molecular, morphological and phylogenetic tools have been used to characterize two populations of Paragonimus located at west of Medellin, Antioquia and at Pichinde, Valle del Cauca (type locality of P. caliensis), Colombia. Adults and metacercariae obtained from Medellin, and metacercariae from Pichinde were analyzed. For morphological observations we used light microscopy and scanning electron microscopy (SEM). Morphology of metacercariae and adults matched with the holotype of P. caliensis. The number and arrangement of sensory papillae in the acetabulum region differs from the morphotypes reported for P. caliensis in Costa Rica. Two morphotypes in branching patterns of ovary and two morphotypes in branching patterns of testes were identified. The main morphological differences between P. caliensis and P. mexicanus corresponded to the size of gonads and their relative positions in the body, and the occasional presence of a cyst wall in P. caliensis metacercariae. The molecular and phylogenetic analyses (using nuclear ribosomal ITS2 and partial cytochrome c oxidase subunit 1 CO1 sequences) confirmed that P. caliensis from the type locality is the same species from Medellin and Costa Rica. Furthermore, these analyses also suggest genetic as well as geographical separation of P. caliensis populations between Colombia and Costa Rica. Currently, P. mexicanus and P. caliensis are sympatric in the Colombian Pacific bioregion, and specific diagnosis based on their egg size is not possible. Therefore, it is necessary to determine the biogeographic distribution ranges of both species and to implement molecular techniques to establish the role of P. caliensis in human paragonimiasis in Colombia.


Assuntos
Braquiúros/parasitologia , Pneumopatias Parasitárias/parasitologia , Metacercárias/genética , Paragonimíase/parasitologia , Paragonimus/fisiologia , Animais , Colômbia , DNA de Helmintos/genética , DNA Espaçador Ribossômico/genética , Humanos , Microscopia Eletrônica de Varredura , Paragonimíase/patologia , Paragonimus/anatomia & histologia , Filogenia , Análise de Sequência de DNA
15.
Parasitology ; 145(6): 792-796, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29113601

RESUMO

Paragonimiasis is an important infectious disease in Chongqing, China. However, no epidemiological surveys of paragonimiasis have been carried out in Chongqing since it became a municipality in 1997. We conducted a retrospective case review of 683 patients who were referred to our laboratory and diagnosed as having paragonimiasis during 2010-2015. Patients were diagnosed with paragonimiasis based on immunodiagnostic tests in addition to clinical and laboratory findings. Patient data extracted from the epidemiologic form were analysed. The majority of patients were distributed on the east side of the Wujiang River, which belongs to the Three Gorges Reservoir region. Consumption of raw or undercooked freshwater crab or crayfish in the family Cambaridae was the main reason for infection. Notably, more than 50·0% of patients were diagnosed between March and July, indicating that serious clinical symptoms only appear approximately 6 months post-infection. Paragonimiasis remains a public health issue in Chongqing, and an epidemiological study of Paragonimus in the Three Gorges region is strongly recommended.


Assuntos
Braquiúros , Paragonimíase/epidemiologia , Paragonimus/isolamento & purificação , Adolescente , Adulto , Animais , Braquiúros/parasitologia , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Incidência , Lactente , Masculino , Paragonimíase/diagnóstico , Paragonimíase/parasitologia , Estudos Retrospectivos , Adulto Jovem , Zoonoses/diagnóstico , Zoonoses/epidemiologia , Zoonoses/parasitologia
16.
J Vet Med Sci ; 79(8): 1419-1425, 2017 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-28717056

RESUMO

Infection of boar-hunting dogs with Paragonimus westermani was investigated in Western Japan. Blood and rectal feces were collected from 441 dogs in the three districts (205 in Kinki, 131 in Chugoku and 105 in Shikoku District). In a screening ELISA for serum antibody against P. westermani antigen, 195 dogs (44.2%) showed positive reaction. In the 195 dogs, 8 dogs were found excreting P. westermani eggs after molecular analysis of fecal eggs, and additional 7 were identified serologically for the parasite infection because of their stronger reactivity against P. westermani antigen than against antigens of other species of Paragonimus. A spatial analysis showed that all of the P. westermani infections were found in Kinki and Chugoku Districts. In this area, dogs' experience of being fed with raw boar meat showed high odds ratio (3.35) to the sero-positivity in the screening ELISA, and the frequency of such experiences was significantly higher in sero-positive dogs. While clear relationship was not obtained between predation of boars by dogs during hunting and their sero-positivity. Therefore, it is suggested that human activity of feeding with wild boar meat is the risk factor for P. westermani infection in boar-hunting dogs. Considering that hunting dogs could play as a major definitive host and maintain the present distribution of P. westermani in Western Japan, control measures for the infection in hunting dogs, such as prohibition of raw meat feeding and regular deworming, should be undertaken.


Assuntos
Doenças do Cão/parasitologia , Carne/parasitologia , Paragonimíase/veterinária , Paragonimus/isolamento & purificação , Sus scrofa/parasitologia , Doenças dos Suínos/parasitologia , Ração Animal/parasitologia , Animais , Doenças do Cão/epidemiologia , Doenças do Cão/etiologia , Cães , Ensaio de Imunoadsorção Enzimática , Japão/epidemiologia , Paragonimíase/epidemiologia , Paragonimíase/parasitologia , Suínos
17.
J Parasitol ; 103(4): 399-403, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28395568

RESUMO

Archaeoparasitological studies on fossilized feces obtained from Joseon Dynasty (1392-1910 CE) mummies have provided invaluable data on the patterns of parasitic infection in pre-modern Korean societies. In our recent radiological investigation of a 17th century Joseon mummy discovered in Cheongdo (South Korea), we located a liver mass just below the diaphragm. Anatomical dissection confirmed the presence of a mass of unknown etiology. A subsequent parasitological examination of a sample of the mass revealed a large number of ancient Paragonimus sp. eggs, making the current report the first archaeoparasitological case of liver abscess caused by ectopic paragonimiasis.


Assuntos
Múmias/parasitologia , Paragonimíase/história , Animais , Autopsia , História do Século XVII , Humanos , Coreia (Geográfico) , Fígado/diagnóstico por imagem , Fígado/parasitologia , Fígado/patologia , Masculino , Pessoa de Meia-Idade , Paragonimíase/diagnóstico por imagem , Paragonimíase/patologia , Paragonimus/isolamento & purificação , República da Coreia , Tomografia Computadorizada por Raios X
18.
Clin Infect Dis ; 64(9): 1271-1274, 2017 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-28158416

RESUMO

Paragonimus kellicotti is an emerging pathogen in the United States with 19 previously reported cases, most in Missouri. Pulmonary symptoms with eosinophilia are most common, though 1 case did involve the central nervous system with few symptoms. We describe the first 2 cases of eosinophilic meningitis due to Paragonimus kellicotti.


Assuntos
Eosinofilia/patologia , Eosinofilia/parasitologia , Meningite/patologia , Meningite/parasitologia , Paragonimíase/diagnóstico , Paragonimíase/patologia , Paragonimus/isolamento & purificação , Adolescente , Adulto , Animais , Eosinofilia/etiologia , Humanos , Masculino , Meningite/etiologia , Estados Unidos
19.
Artigo em Chinês | MEDLINE | ID: mdl-30133238

RESUMO

Objective: To perform a proteomics analysis for metacercariae, juvenile and adult worms of Paragonimus skrjabini. Methods: Crabs were collected in P. skrjabini endemic areas of Kaiyang and Bijie in Guizhou Province. Metacercariae were isolated, placed in PBS, and were used to infect three SD rats(10 metacercariae/rat) by intragastric administration and infect three male dogs(20 metacercariae/dog) through feeding. The rats were sacrificed at 1 month after infection to obtain juvenile worms. The dogs were sacrificed at 3 months after infection to obtain adult worms. The metacercariae, juvenile and adult worms were lysed, and total protein was extracted by ultrasonication. The total protein content was determined by Bradford method and separated by SDS-PAGE and two dimensional gel electrophoresis. Images of two dimensional gel electrophoresis were analyzed using the PDquest 8.0 software. The dots with difference were digested and analyzed with mass spectrometry. Finally, online searches in NCBI and local databases were performed. Results: Results of SDS-PAGE showed that the total protein of metacercariae, juvenile and adult worms was concentrated within Mr of 25 000-116 000. Fifty-one protein dots with difference were found by two dimensional gel electrophoresis, comprising of 20 dots for metacercariae, 25 for juvenile worms and 6 for adult worms. Thirty-six peptide sequences of metacercariae and juvenile worms were analyzed. They were basically determined to be Achrornobacter lyticus protease Ⅰ(a lysine-specific serine protease), ascorbate reductase protein, glutathione s-transferase DHAR2 analogue, heat shock proteins Hsp82 and Hsp96-ß, actin, cystatin, etc., by online searches, and cysteine, actin and heat shock protein by local searches in the diginea database(downloaded to a local computer from NCBI). Mass spectrometry was not performed for adult worms, as the variation of grayscale value between their spots was far less than those for metacercariae and juvenile worms. Conclusion: One difference is that the metacercariae of P. skrjabini have actin, while the juvenile worms have detoxification proteins and stress proteins. But they both have hydrolases and cysteine enzymes.


Assuntos
Paragonimíase , Paragonimus , Animais , Braquiúros , Masculino , Proteômica , Ratos , Ratos Sprague-Dawley
20.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 29(5): 579-582, 2017 Jul 05.
Artigo em Chinês | MEDLINE | ID: mdl-29469352

RESUMO

OBJECTIVE: To understand the current status of paragonimiasis epidemic in western mountain areas in Hubei Province. METHODS: Four counties (cities) of Western Hubei Province (Xingshan, Enshi, Yunxi, Baokang) were selected as the investigation sites for active surveillance. Crabs were captured and the metacercariae of Paragonimus were detected. Meanwhile, the blood samples were collected from the residents in the surveillance sites and the unique IgG and IgM antibodies against Paragonimus in the sera were detected by ELISA. In addition, a questionnaire survey about knowledge and behavior of prevention and control of paragonimiasis was taken among the residents. RESULTS: A total of 1 143 residents were investigated in the active surveillance, the total positive rate of the serology test was 1.84% (21/1 143), while the rates of the male and the female were 1.78% (10/562) and 1.89% (11/581), respectively, with no statistical significance between them ( χ2 = 0.002, P > 0.05). The average weight of 161 fresh-water crabs captured was 11.72 g, with the positive rate of 9.32% (15/161) and the infective density of 7.07 metacercariae per positive crab. The positive rates of the male and female crabs were 11.54% (9/78) and 7.23% (6/83), respectively ( χ2 = 0.884, P > 0.05), and the infective densities were 6.67 and 7.67 metacercariae per positive crab, respectively. Totally 1 143 residents were investigated by questionnaires, and 0.44% of them had the behavior of eating raw or half-done fresh-water crab, and 0.87% of them had the behavior of drinking un-boiled stream water. CONCLUSIONS: The transmission chain of paragonimiasis still exists in the nature environment of mountain area in Western Hubei Province. The positive rate of the second intermediate host rebounds in some investigation sites. Therefore, the measures of continuous surveillance and health education should be taken to avoid the appearance of the prevalence or outbreak.


Assuntos
Braquiúros/parasitologia , Paragonimíase/epidemiologia , Animais , Anticorpos Anti-Helmínticos/sangue , China/epidemiologia , Epidemias , Feminino , Água Doce , Humanos , Masculino , Paragonimus , Inquéritos e Questionários
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