Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 116
Filtrar
Mais filtros










Filtros aplicados

Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
Artigo em Chinês | MEDLINE | ID: mdl-32185925

RESUMO

OBJECTIVE: To compare the complete mitochondrial genome sequences of two phenotypes of Paragonimus westermani isolated from Fujian Province with different sizes of metacercariae, and perform a phylogenetic analysis of various geographical isolates of P. westermani from Asia, so as to identify the possible genetic characteristics associated with the P. westermani phenotypes. METHODS: P. westermani metacercariae with different sizes (large metacercariae, 380-420 µm in diameter; small metacercariae, 320-340 µm) isolated from freshwater crabs were used to infect dogs, and the eggs and adult worms of P. westermani were collected from the dog stool samples and lung tissues. Then, the egg size and morphology were compared. In addition, genomic DNA was extracted from the adult worms of the two phenotypes of P. westermani and used for the PCR amplification to yield the complete mitochondrial genome sequence. Sequence structure and phylogenetic analyses were performed based on the complete mitochondrial genome of P. westermani. RESULTS: Following infection with large and small P. westermani metacercariae, the adult worms recovered from the dog lung had a thick body, and had oral and ventral suckers. The ventral sucker was located slightly in front of the midline of the body, and testes, ovary and vitelline gland were seen in the adult worms. Following fixation, the adults appeared oval, with an approximately 1.7∶1 of the length-width ratio. The length and width of the eggs isolated from the fecal samples of dogs infected with large and small P. westermani metacercariae varied significantly, and the large metacercariae produced bigger eggs than the smaller metacercariae. Based on the morphological features of adults and eggs and the ITS2 sequences, both phenotypes were identified as P. westermani. The complete mitochondrial genome sequence analysis of adults showed almost consistent sequences in the protein-coding region of the mitochondrial genome of adult worms derived from large and small metacercariae, with a major variation seen in the former non-coding region. Sliding window analysis revealed the most polymorphic region within the ND4 gene across the mitochondrial genome from various geographical isolates of P. westermani, and phylogenetic analysis showed that both phenotypes were clustered into the Chinese branch of P. westermani, which was close to the Japanese branch and distinct from the South/Southeast Asian branch. CONCLUSIONS: The genetic distance between the phenotypes of P. westermani isolated from Fujian Province is near at a mitochondrial genome level, with no remarkable genetic differentiation seen; however, the mutation and structural changes in the non-coding region may result in the phenotypic variations. In addition, there is a distinct variation of the evolutionary rate in the mitochondrial coding genes, suggesting the selection of appropriate molecular markers during the phylogenic researches.


Assuntos
Genoma Mitocondrial , Paragonimus westermani , Filogenia , Animais , Ásia , China , DNA de Helmintos/genética , Cães , Feminino , Genoma Mitocondrial/genética , Paragonimus westermani/classificação , Paragonimus westermani/genética
2.
Acta Trop ; 200: 105185, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31542373

RESUMO

Paragonimus westermani (P. westermani) is widely spread in Asian countries and is one of the most important causative agents for lung fluke diseases. The prevention and control of Paragonimiaisis mainly depends on the accurate diagnosis and effective treatment. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay targeted to a portion of the Ty3/gypsy-like LTR retrotransposon (Rn1) sequence coupled with a lateral flow dipstick (LFD) for the rapid detection of P. westermani-specific amplicons. The positive LAMP products were biotin-labeled and hybridized with a fluorescein isothiocyanate-labeled probe which could be visually detected by LFD. No cross-reaction were observed with other parasitic pathogens including Trichinella spiralis, Anisakis simplex, Schistosoma japonicum and Gnathostoma spinigerum, but this LAMP assay could not distinguish P. westermani with Paragonimus skrjabini and Paragonimus heterotremus. The detection limit of the LAMP assay for P. westermani was 2.7 fg/µL, while that of PCR method was 27 fg/µL. LAMP method was applied to detect P. westermani genomic DNA in blood samples form experimental infected dogs, and results showed the parasite was detectable as early as week 2. LAMP-LFD assay applicability was successfully tested in dog blood samples collected from five cities (Wenzhou, Hangzhou, Huzhou, Jiaxing and Shaoxing) in Zhejiang province. In summary, the established LAMP-LFD assay targeted to the Rn1 sequence is a rapid and convenient method for specific detection of P. westermani.


Assuntos
Primers do DNA/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Paragonimíase/diagnóstico , Paragonimíase/genética , Paragonimus westermani/genética , Paragonimus westermani/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Animais , China/epidemiologia , Cães , Paragonimíase/epidemiologia , Sensibilidade e Especificidade
3.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(2): 200-203, 2019 Apr 29.
Artigo em Chinês | MEDLINE | ID: mdl-31184058

RESUMO

OBJECTIVE: To analyze the clinical manifestations and imaging characteristics of pulmonary and extra pulmonary paragonimiasis westermani. METHODS: A retrospective analysis was performed of 30 patients diagnosed by clinical features, laboratory serological tests and surgical pathology. RESULTS: The symptoms of the lung included mainly chest distress, fever, chest pain, cough and expectoration, and dyspnea. The extra pulmonary symptoms included abdominal pain, vomiting, diarrhea, poor appetite, emaciation, both lower extremities asthenia, headache, dizziness, epileptic seizures, and subcutaneous migratory masses. The laboratory examination showed that the eosinophil numbers of serum and pleural effusion of all the thirty patients were increased, and the eggs of Paragonimus westermani were found by the stool tests in four cases. The chest CT tests found abnormal nodules, ground glass changes, insect damages, pleural effusion, "tunnel" signs, and "halo" signs. Cranial CT and MRI showed intracranial hemorrhage foci, and extensive "finger-like" edema. Abdominal CT showed serpentine deformation and "tunnel" signs in the hepatic and spleen capsules. CONCLUSIONS: Paragonimiasis westermani is a multiple organ system involved infection, and it has complex and varied clinical manifestations. The "tunnel" sign and serpentine deformations in the intracranial part, lung, liver and spleen are important imaging manifestations of the disease.


Assuntos
Paragonimíase , Paragonimus westermani , Tomografia Computadorizada por Raios X , Animais , Fezes/parasitologia , Humanos , Paragonimíase/diagnóstico por imagem , Paragonimíase/patologia , Derrame Pleural , Estudos Retrospectivos
4.
Mem Inst Oswaldo Cruz ; 114: e180595, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31116227

RESUMO

The genetic information of ancient Paragonimus westermani, the oriental lung fluke infecting over 20 million people worldwide, has not been thoroughly investigated thus far. We analysed genetic markers (COI and ITS2) of P. westermani from coprolite specimens (n = 6) obtained from 15th to 18th century Korean mummies. Our results indicated that all P. westermani sequences were generally distinct from the other species of the genus Paragonimus. The sequences were clustered into three groups: Group I for East Asia; Group II for South and Southeast Asia; and Group III for India and Sri Lanka. In this study, we found that ancient P. westermani sequences in Korea belong to Group I, adding invaluable information to the existing knowledge of Paragonimus paleogenetics.


Assuntos
DNA de Helmintos/genética , DNA Espaçador Ribossômico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Fezes/parasitologia , Múmias/parasitologia , Paragonimus westermani/isolamento & purificação , Animais , Ásia , Humanos , Paleodontologia , Paragonimus westermani/genética , Contagem de Ovos de Parasitas , Filogenia
8.
J Infect Chemother ; 25(3): 200-203, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30213500

RESUMO

We report a case of Paragonimus westermani infection simultaneously affecting two separate organs that presented as both a pulmonary cavity and adrenal mass in an immunocompromised host. A 65-year-old male with a previous kidney transplant visited our clinic because of hemoptysis. Chest computed tomography (CT) showed a pulmonary cavity and right adrenal gland mass. The Aspergillus antigen titer in bronchial lavage fluid was elevated and showed positive conversion. It was necessary to differentiate lung cancer with adrenal gland metastasis from a fungal infection with an adrenal gland adenoma. Positron emission tomography CT suggested benign disease, and it was misdiagnosed as pulmonary aspergillosis based on the elevated Aspergillus antigen titer in the bronchial lavage fluid. Owing to the adverse effects of anti-fungal treatment, the patient underwent wedge resection of the lung and P. westermani was confirmed. A careful history revealed that the patient had eaten raw freshwater crabs 3 years earlier, and a test for serum antibodies to P. westermani was positive. Despite treatment with praziquantel, the adrenal mass persisted on 3-month follow-up CT. A right adrenalectomy was performed and a P. westermani infection was confirmed.


Assuntos
Doenças das Glândulas Suprarrenais , Pneumopatias , Paragonimíase , Paragonimus westermani , Doenças das Glândulas Suprarrenais/diagnóstico por imagem , Doenças das Glândulas Suprarrenais/cirurgia , Glândulas Suprarrenais/diagnóstico por imagem , Glândulas Suprarrenais/parasitologia , Glândulas Suprarrenais/cirurgia , Idoso , Animais , Diagnóstico Diferencial , Doenças Transmitidas por Alimentos/diagnóstico por imagem , Doenças Transmitidas por Alimentos/cirurgia , Humanos , Pulmão/diagnóstico por imagem , Pulmão/parasitologia , Pulmão/cirurgia , Pneumopatias/diagnóstico por imagem , Pneumopatias/cirurgia , Masculino , Paragonimíase/diagnóstico por imagem , Paragonimíase/cirurgia , Radiografia Torácica , Tomografia Computadorizada por Raios X
9.
Gigascience ; 8(1)2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-30520948

RESUMO

Background: Foodborne infections caused by lung flukes of the genus Paragonimus are a significant and widespread public health problem in tropical areas. Approximately 50 Paragonimus species have been reported to infect animals and humans, but Paragonimus westermani is responsible for the bulk of human disease. Despite their medical and economic importance, no genome sequence for any Paragonimus species is available. Results: We sequenced and assembled the genome of P. westermani, which is among the largest of the known pathogen genomes with an estimated size of 1.1 Gb. A 922.8 Mb genome assembly was generated from Illumina and Pacific Biosciences (PacBio) sequence data, covering 84% of the estimated genome size. The genome has a high proportion (45%) of repeat-derived DNA, particularly of the long interspersed element and long terminal repeat subtypes, and the expansion of these elements may explain some of the large size. We predicted 12,852 protein coding genes, showing a high level of conservation with related trematode species. The majority of proteins (80%) had homologs in the human liver fluke Opisthorchis viverrini, with an average sequence identity of 64.1%. Assembly of the P. westermani mitochondrial genome from long PacBio reads resulted in a single high-quality circularized 20.6 kb contig. The contig harbored a 6.9 kb region of non-coding repetitive DNA comprised of three distinct repeat units. Our results suggest that the region is highly polymorphic in P. westermani, possibly even within single worm isolates. Conclusions: The generated assembly represents the first Paragonimus genome sequence and will facilitate future molecular studies of this important, but neglected, parasite group.


Assuntos
Genoma Helmíntico , Paragonimus westermani/genética , Sequenciamento Completo do Genoma/métodos , Animais , Tamanho do Genoma , Genoma Mitocondrial , Sequenciamento de Nucleotídeos em Larga Escala , Anotação de Sequência Molecular , Filogenia , Homologia de Sequência do Ácido Nucleico
10.
J Stroke Cerebrovasc Dis ; 27(10): 2648-2649, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30031686

RESUMO

Paragonimiasis is a food-borne parasitic disease caused by Paragonimus lung flukes, which are epidemic in Asia. Cerebral paragonimiasis accounts for <1% of symptomatic paragonimiasis but is the most common extrapulmonary infection. Cerebral paragonimiasis often mimics stroke and sometimes causes severe neurological sequelae. A 61-year-old woman was admitted to the hospital for severe headache. A head computed tomography scan revealed intracerebral hemorrhage with subarachnoid hemorrhage. The patient also had lesions in the lungs. She frequently ate Japanese mitten crab. Peripheral blood examination results of increased eosinophilia and immunological testing results confirmed the diagnosis of Paragonimus westermani infection. The patient was successfully treated with praziquantel as the first-line agent. Cerebral paragonimiasis is currently rare in developed countries; however, it is an important disease to consider.


Assuntos
Helmintíase do Sistema Nervoso Central/parasitologia , Hemorragia Cerebral/parasitologia , Países Desenvolvidos , Paragonimíase/parasitologia , Paragonimus westermani/isolamento & purificação , Acidente Vascular Cerebral/parasitologia , Hemorragia Subaracnóidea/parasitologia , Animais , Anti-Helmínticos/uso terapêutico , Helmintíase do Sistema Nervoso Central/diagnóstico , Helmintíase do Sistema Nervoso Central/tratamento farmacológico , Hemorragia Cerebral/diagnóstico , Feminino , Humanos , Japão , Imagem por Ressonância Magnética , Pessoa de Meia-Idade , Paragonimíase/diagnóstico , Paragonimíase/tratamento farmacológico , Praziquantel/uso terapêutico , Acidente Vascular Cerebral/diagnóstico , Hemorragia Subaracnóidea/diagnóstico , Tomografia Computadorizada por Raios X , Resultado do Tratamento
11.
Paediatr Int Child Health ; 38(4): 302-307, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-28884631

RESUMO

An 11-year-old boy collapsed during morning assembly at his junior high school. The automated external defibrillator detected ventricular fibrillation and provided shock delivery. He was successfully resuscitated and reverted to sinus rhythm. Electrocardiography showed ST-T elevation in the precordial leads. Echocardiography and angiography demonstrated akinesia of the apex and mid-wall of the left ventricle with preserved contraction of the basal segments, which suggested Takotsubo cardiomyopathy. The patient and his family had often eaten uncooked crab, and his father had a past history of infection with Paragonimiasis westermani. The patient had had a persistent cough and chest pain for several weeks. Chest radiograph showed cystic cavities in the left upper lung. Microbiological examination of the sputum demonstrated an egg of P. westermani and immunological assay showed a raised antibody titre to P. westermani. On the12th day of admission, he developed seizures, and magnetic resonance imaging demonstrated cerebral involvement. After the administration of praziquantel for 3 days, the clinical manifestations improved immediately, and echocardiography normalised within 3 weeks. The patient was discharged on the 32nd day + and follow-up was normal. Takotsubo cardiomyopathy following a potentially fatal arrhythmia is a rare cardiac complication associated with pulmonary and central nervous system infection by P. westermani.


Assuntos
Paragonimíase/complicações , Paragonimíase/diagnóstico , Cardiomiopatia de Takotsubo/complicações , Cardiomiopatia de Takotsubo/diagnóstico , Angiografia , Animais , Antiparasitários/administração & dosagem , Infecções Parasitárias do Sistema Nervoso Central/diagnóstico , Infecções Parasitárias do Sistema Nervoso Central/patologia , Criança , Ecocardiografia , Eletrocardiografia , Humanos , Imagem por Ressonância Magnética , Masculino , Técnicas Microbiológicas , Paragonimíase/tratamento farmacológico , Paragonimíase/patologia , Paragonimus westermani/imunologia , Praziquantel/administração & dosagem , Radiografia Torácica , Cardiomiopatia de Takotsubo/patologia , Resultado do Tratamento
12.
Korean J Parasitol ; 55(3): 313-317, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28719956

RESUMO

Paragonimiasis is a parasitic disease caused by Paragnonimus species. The primary site of infection is the lung, and extrapulmonary involvement is also reported. When infected with Paragonimus westermani, which is the dominant species in Korea, the central nervous system is frequently involved along with the liver, intestine, peritoneal cavity, retroperitoneum, and abdominal wall. Ectopic paragonimiasis raises diagnostic challenge since it is uncommon and may be confused with malignancy or other inflammatory diseases. Here, we report an ectopic paragonimiasis case initially presented with recurrent abdominal pain. The patient developed abdominal pain 3 times for the previous 3 years and the computed tomography (CT) of the abdomen revealed fluid collection with wall enhancement. Recurrent diverticulitis was initially suspected and part of the ascending colon was resected. However, the specimen showed intact colon wall without evidence of diverticulitis and multiple parasite eggs and granulomas were found instead. The size of about 70 µm, the presence of an operculum and relatively thick egg shell suggested eggs of Paragonimus species. With appropriate exposure history and a positive antibody test, the definitive diagnosis was made as peritoneal paragonimiasis.


Assuntos
Doenças do Colo/diagnóstico , Enteropatias Parasitárias/diagnóstico , Paragonimíase/diagnóstico , Abscesso Abdominal , Dor Abdominal/etiologia , Animais , Doenças do Colo/complicações , Doenças do Colo/parasitologia , Diagnóstico Diferencial , Doença Diverticular do Colo , Humanos , Enteropatias Parasitárias/complicações , Enteropatias Parasitárias/parasitologia , Masculino , Pessoa de Meia-Idade , Paragonimíase/complicações , Paragonimíase/parasitologia , Paragonimus westermani/isolamento & purificação , Contagem de Ovos de Parasitas , Recidiva
13.
Korean J Parasitol ; 55(3): 347-350, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28719962

RESUMO

An epidemiological study was performed to know the recent infection status of Paragonimus westermani metacercariae (PwMc) in freshwater crayfish, Cambaroides similis, from 2 streams in Jeollanam-do, Republic of Korea. Crayfish were collected from creeks in Bogil-do (Island), Wando-gun, and in a creek near Daeheung Temple in Haenam-gun. The infection rate of crayfish with PwMc in Bogil-do was 89.8%, and the metacercarial burden was 37 PwMc per the infected crayfish. Crayfish in a creek near Daeheung Temple were larger and twice heavier than those in Bogil-do. Of them, 96.5% were infected with PwMc. An average of 140 metacercariae was found in the infected crayfish, almost quadruple to those of Bogil-do. There was a strong correlation between the number of PwMc and body weight of the crayfish. These results suggest that P. westermani metacercariae are still prevalent in crayfish of the 2 regions in Jeollanam-do, Korea.


Assuntos
Astacoidea/parasitologia , Água Doce , Metacercárias/isolamento & purificação , Paragonimus westermani/isolamento & purificação , Animais , Astacoidea/anatomia & histologia , Peso Corporal , Incidência , Paragonimíase/epidemiologia , República da Coreia/epidemiologia
14.
Korean J Parasitol ; 55(3): 357-361, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28719964

RESUMO

During civil engineering construction near Sejong-ro, Jongro-ku, Seoul, cultural sites were found that are thought to have been built in the 15th century. This area was home to many different people as well as the leaders of the Yi dynasty. To gain further insight into the life styles of the inhabitants of the old capital, soil samples were collected from various areas such as toilets, water foundations, and drainage ways. Parasite eggs were examined by microscopy after 5 g soil samples were rehydrated in 0.5% trisodium phosphate solution. A total of 662 parasite eggs from 7 species were found. Species with the highest number of eggs found were Ascaris lumbricoides (n=483), followed by Trichuris trichiura (138), Trichuris vulpis (21), Fasciola hepatica (8), Clonorchis sinensis (6), Paragonimus westermani (4), and Metagonimus yokogawai (2). These findings indirectly indicate the food habits of the people in Yi dynasty.


Assuntos
Arqueologia , Comportamento Alimentar , Estilo de Vida/história , Contagem de Ovos de Parasitas , Parasitologia , Solo/parasitologia , Animais , Ascaris lumbricoides , Clonorchis sinensis , Fasciola hepatica , Heterophyidae , História do Século XV , Humanos , Paragonimus westermani , República da Coreia , Trichuris
15.
JNMA J Nepal Med Assoc ; 56(206): 268-270, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28746328

RESUMO

Paragonimiasis is a zoonosis caused by many species of Paragonimus commonly P. westermani. Human get infected by eating raw, salted, pickled, smoked, partially cooked crustaceans (crayfish or crabs). Clinical manifestations ranges from non-specific symptoms like pain abdomen, diarrhea, urticarial rashes, fever to pleuropulmonary symptoms like cough, hemoptysis, chest pain and dyspnea. A 48 years, female presented at TUTH emergency with fever on and off for nine months, cough and shortness of breath for three months, lethargy, malaise and urticaria with history of raw crab intake one month prior to the onset of symptoms. Blood and pleural fluid analysis revealed raised total counts with eosinophilia and x-ray showed bilateral infiltration of lower lobes with pleural effusion. Diagnosis was confirmed by microscopic examination of sputum for Paragonimus. She responded well to Praziquantel. Pulmonary paragonimiasis must be considered in the differential diagnosis of unresolving pneumonia and unexplained hypereosinophilia.


Assuntos
Eosinofilia , Paragonimíase , Paragonimus westermani/isolamento & purificação , Derrame Pleural , Pneumonia , Praziquantel/administração & dosagem , Frutos do Mar , Animais , Anti-Helmínticos/administração & dosagem , Diagnóstico Diferencial , Eosinofilia/diagnóstico , Eosinofilia/etiologia , Feminino , Doenças Transmitidas por Alimentos/diagnóstico , Doenças Transmitidas por Alimentos/tratamento farmacológico , Doenças Transmitidas por Alimentos/fisiopatologia , Humanos , Pessoa de Meia-Idade , Paragonimíase/diagnóstico , Paragonimíase/etiologia , Paragonimíase/fisiopatologia , Paragonimíase/terapia , Derrame Pleural/sangue , Derrame Pleural/diagnóstico , Derrame Pleural/etiologia , Derrame Pleural/terapia , Pneumonia/sangue , Pneumonia/diagnóstico , Pneumonia/tratamento farmacológico , Pneumonia/etiologia , Escarro/microbiologia , Avaliação de Sintomas/métodos , Resultado do Tratamento
16.
J Biol Chem ; 292(21): 8667-8682, 2017 05 26.
Artigo em Inglês | MEDLINE | ID: mdl-28348084

RESUMO

MF6p/FhHDM-1 is a small protein secreted by the parasitic flatworm (trematode) Fasciola hepatica that belongs to a broad family of heme-binding proteins (MF6p/helminth defense molecules (HDMs)). MF6p/HDMs are of interest for understanding heme homeostasis in trematodes and as potential targets for the development of new flukicides. Moreover, interest in these molecules has also increased because of their immunomodulatory properties. Here we have extended our previous findings on the mechanism of MF6p/HDM-heme interactions and mapped the protein regions required for heme binding and for other biological functions. Our data revealed that MF6p/FhHDM-1 forms high-molecular-weight complexes when associated with heme and that these complexes are reorganized by a stacking procedure to form fibril-like and granular nanostructures. Furthermore, we showed that MF6p/FhHDM-1 is a transitory heme-binding protein as protein·heme complexes can be disrupted by contact with an apoprotein (e.g. apomyoglobin) with higher affinity for heme. We also demonstrated that (i) the heme-binding region is located in the MF6p/FhHDM-1 C-terminal moiety, which also inhibits the peroxidase-like activity of heme, and (ii) MF6p/HDMs from other trematodes, such as Opisthorchis viverrini and Paragonimus westermani, also bind heme. Finally, we observed that the N-terminal, but not the C-terminal, moiety of MF6p/HDMs has a predicted structural analogy with cell-penetrating peptides and that both the entire protein and the peptide corresponding to the N-terminal moiety of MF6p/FhHDM-1 interact in vitro with cell membranes in hemin-preconditioned erythrocytes. Our findings suggest that MF6p/HDMs can transport heme in trematodes and thereby shield the parasite from the harmful effects of heme.


Assuntos
Proteínas de Transporte/química , Fasciola hepatica/química , Proteínas de Helminto/química , Heme/química , Opisthorchis/química , Paragonimus westermani/química , Animais , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Bovinos , Fasciola hepatica/genética , Fasciola hepatica/metabolismo , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Heme/metabolismo , Opisthorchis/genética , Opisthorchis/metabolismo , Paragonimus westermani/genética , Paragonimus westermani/metabolismo , Domínios Proteicos
17.
Parasitol Res ; 116(3): 1003-1006, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28127717

RESUMO

Soy sauce-marinated freshwater crabs (Eriocheir japonicus) are a source of human paragonimiasis. The viability of Paragonimus westermani metacercariae (PwMc) in marinated crabs was investigated in an experimental setting. The PwMc collected from freshwater crayfish were inoculated into freshwater crabs, which were then frozen or marinated in soy sauce. All PwMc in the freshwater crabs were inactivated after freezing for 48 h at -20 °C and after freezing for 12 h at -40 °C. After marinating for 32 days, the survival rate of PwMc in 5% NaCl soy sauce was 50%, in 7.5% NaCl soy sauce it was 33.3%, and in 10.0% NaCl soy sauce it was 31.3%. When marinated for 64 days, all PwMc were inactivated in all experimental groups. These results revealed that freezing and soy sauce marination were detrimental to the survival of PwMc in freshwater crabs. Specifically, freezing crabs for more than 48 h or soaking them in soy sauce containing at least 5.0% NaCl for 64 days can inactivate PwMc. These results can inform the production of the traditional Korean soy sauce-marinated freshwater crabs known as gejang.


Assuntos
Contaminação de Alimentos/prevenção & controle , Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Paragonimíase/prevenção & controle , Paragonimus westermani/fisiologia , Frutos do Mar/parasitologia , Animais , Contaminação de Alimentos/análise , Conservantes de Alimentos/análise , Água Doce/parasitologia , Humanos , Paragonimíase/parasitologia , Paragonimíase/transmissão , Paragonimus westermani/efeitos dos fármacos , Paragonimus westermani/isolamento & purificação , Frutos do Mar/análise , Cloreto de Sódio/análise , Cloreto de Sódio/farmacologia , Alimentos de Soja/análise
18.
J Bronchology Interv Pulmonol ; 24(3): 241-243, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27070338

RESUMO

Surgical resection is traditionally the preferred treatment for fluorodeoxyglucose-avid peripheral pulmonary nodules that grow over time. However, new technologies, including electromagnetic navigational bronchoscopy (ENB), provide an opportunity to confirm or possibly exclude a cytologic cancer diagnosis, before resection. We present a case of a 56-year-old North American man who presented with a fluorodeoxyglucose-avid pulmonary nodule and sought a second opinion after being recommended thoracotomy with lobectomy. The peripheral nodule was biopsied using ENB and pathologic evaluation of the lesion demonstrated parasitic eggs with features of Paragonimus westermani. No evidence of malignancy was found. The radiographic abnormalities resolved after treatment with praziquantel. Using a minimally invasive procedure with ENB, we successfully diagnosed pulmonary infection with P. westermani, a rare infectious cause of peripheral pulmonary lesions in a patient without travel to an endemic area. Furthermore, an alternative diagnosis to cancer was established, sparing this patient an unnecessary thoracotomy with right middle lobectomy.


Assuntos
Pneumopatias/diagnóstico por imagem , Paragonimíase/diagnóstico por imagem , Paragonimus westermani/isolamento & purificação , Animais , Biópsia , Broncoscopia , Tosse/etiologia , Diagnóstico Diferencial , Humanos , Pneumopatias/complicações , Pneumopatias/patologia , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Paragonimíase/complicações , Paragonimíase/patologia
19.
Intern Med ; 55(19): 2889-2892, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27725555

RESUMO

A 42-year-old woman presented with an intermittent fever and chest and back pain, and an abnormal chest shadow was detected. She was diagnosed with paragonimiasis caused by Paragonimus westermani. Praziquantel therapy improved the abnormal chest shadow, but did not relieve her symptoms. She was also diagnosed with familial Mediterranean fever (FMF), and colchicine therapy resolved her symptoms. She subsequently developed arthralgia and morning stiffness in her hands. We also diagnosed the patient with rheumatoid arthritis (RA), and corticosteroid and salazosulfapyridine therapy improved her symptoms. The existence of paragonimiasis complicated the diagnosis of FMF. The coexistence of FMF and RA is very rare, but does exist.


Assuntos
Artrite Reumatoide/complicações , Febre Familiar do Mediterrâneo/complicações , Febre Familiar do Mediterrâneo/diagnóstico , Paragonimíase/complicações , Corticosteroides/uso terapêutico , Adulto , Animais , Artrite Reumatoide/tratamento farmacológico , Colchicina/uso terapêutico , Febre Familiar do Mediterrâneo/tratamento farmacológico , Feminino , Humanos , Paragonimíase/tratamento farmacológico , Paragonimus westermani , Praziquantel/uso terapêutico , Sulfassalazina
20.
Parasitol Int ; 65(6 Pt A): 607-612, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27644890

RESUMO

Paragonimiasis is a typical food-borne parasitic disease, endemic in most parts of Asia, with sporadic case reports from American and African countries. The major source of infection is undercooked freshwater crab or crayfish, though consumption of wild boar meat is also responsible for the infection in Japan, because wild boar is a paratenic host for Paragonimus westermani. Recently, living juveniles of P. westermani were isolated from muscle of a sika deer, Cervus nippon, in Japan, raising the possibility that venison has been another source of infection. In order to clarify the potential contribution of venison consumption to the occurrence of paragonimiasis, we analysed dietary histories of those paragonimiasis patients in whose diagnoses we were involved between 2001 and 2015. Among 380 patients, freshwater crab had been consumed by 208 patients, wild boar meat by 190, and wild deer meat by 76 patients before the onset of the disease. Overall contribution of wild deer meat was estimated to be 6.8% to 20.0%, although in Oita and Gifu Prefectures, where a substantial proportion of patients had consumed raw venison, the contribution of venison consumption was much higher (27.5 to 62.1% and 42.1 to 78.9% in Oita and Gifu Prefectures, respectively). We demonstrated P. westermani-specific antibodies in the sera of 4 out of 160 sika deer from Gifu Prefecture, strongly suggesting that these deer were infected with P. westermani.


Assuntos
Anticorpos Antiprotozoários/sangue , Doenças Transmitidas por Alimentos/parasitologia , Paragonimíase/epidemiologia , Paragonimus westermani/isolamento & purificação , Carne Vermelha/parasitologia , Animais , Antígenos de Protozoários/imunologia , Cervos/parasitologia , Japão/epidemiologia , Músculo Esquelético/parasitologia , Paragonimíase/parasitologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA