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1.
Nat Prod Res ; 34(3): 445-448, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30602287

RESUMO

An experimental investigation on essential oil of a Montenegrin Helichrysum italicum (Roth) G. Don fil. (Asteraceae) is reported. The essential oil was analysed in both liquid and vapour phases. Gas chromatography-mass spectrometry analysis of the liquid oil showed the predominance of sesquiterpenes with ß-eudesmene (21.65%) and ß-bisabolene (19.90%) as the major ones. Monoterpene fraction was mainly represented by α-pinene (16.90%) and neryl acetate (10.66%). Head-space technique revealed the vapour phase enriched of monoterpenes with α-pinene (78.76%) predominance. The essential oil was tested against methicillin-sensitive Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Candida albicans (ATCC 14053) and the clinical strains of methicillin-resistant S. aureus, carbapenem-resistant Klebsiella pneumoniae, carbapenem-resistant Acinetobacter baumannii and carbapenem-resistant Pseudomonas aeruginosa. Interesting fungicidal/bactericidal potency against C. albicans and carbapenem-resistant A. baumannii was revealed at concentration of 5% v/v.


Assuntos
Antibacterianos/química , Helichrysum/química , Óleos Voláteis/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Asteraceae , Bactérias/efeitos dos fármacos , Monoterpenos Bicíclicos , Candida/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Sesquiterpenos Monocíclicos , Montenegro , Óleos Voláteis/farmacologia , Terpenos/química , Terpenos/isolamento & purificação , Terpenos/farmacologia
2.
J Med Food ; 22(10): 1041-1046, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31199702

RESUMO

The chemical composition of the essential oil of flowering aerial parts of Helichrysum italicum subsp. italicum cultivated in central Italy, Marche region, was analyzed by means of gas chromatography-mass spectrometry. Seventy-eight components, accounting for 98.71% of the whole essential oil composition, were identified and quantified. Neryl acetate showed the largest relative abundance in the composition, accounting for 15.75% of the oil, followed by α-pinene (8.21%); 4,6,9-trimethyl-8-decene-3,5-dione, (Italidione I), (7.34%); ar-curcumene and ß-selinene (5.37%); γ-curcumene (4.83%); nerol (4.75%); α-selinene (4.68%); limonene (4.55%); linalool (4.42%), and 2,4,6,9-tetramethyl-8-decene-3,5-dione (Italidione II), (4.26%). The oil inhibited in vitro collagenase and elastase activities, with IC50 values of 36.99 ± 1.52 and 135.43 ± 6.32 µg/mL, respectively. Neryl acetate, nerol, and linalool, distinctive compounds of the oil obtained from this plant, tested alone or in mixture, at the same percentages shown in the essential oil, exhibited no activity against the two enzymes. On the contrary, α-pinene and limonene, tested alone and in mixture, showed inhibitory activity on both collagenase and elastase.


Assuntos
Helichrysum/química , Inibidores de Metaloproteinases de Matriz/farmacologia , Óleos Voláteis/farmacologia , Elastase Pancreática/antagonistas & inibidores , Óleos Vegetais/farmacologia , Acetatos , Monoterpenos Acíclicos , Monoterpenos Bicíclicos , Colagenases , Itália , Limoneno
3.
J Med Microbiol ; 68(5): 703-710, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30916640

RESUMO

OBJECTIVE: The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis and Helichrysum italicum essential oils (EO). METHODS: Antimycobacterial potential was tested against Mycobacterium avium and Mycobacterium intracellulare using broth and water dilution method and checkerboard synergy method. Antiadhesion and antibiofilm effect of EOs was evaluated on biotic (HeLa cells) and abiotic surface (polystyrene). To evaluate the possible mechanisms of action, cellular leakage of proteins and DNA was tested and structural changes were visualized with a transmission electron microscope. RESULTS: MIC, minimum bactericidal concentration (MBC) and minimal effective concentration (MEC) were 1.6 mg ml-1 for J. communis EO and 3.2 mg ml-1 for H. italicum EO against both mycobacteria. All combinations of EOs in checkerboard synergy method produced fractional inhibitory concentration index values ranging from 0.501 to 1.5, corresponding to synergistic, additive or indifferent effects. Mycobacterium avium showed a greater tendency to create biofilm but these EOs at subinhibitory concentrations (sMIC) effectively blocked the adhesion and the establishment of biofilm. The exposure of both mycobacteria to MICs and sMICs lead to significant morphological changes: acquired a swollen form, ghost-like cell, disorganized cytoplasm detached from the cell wall. OD value of supernatant for both mycobacteria exposed to EOs have confirmed that there is a leakage of cellular material. CONCLUSION: The leakage of the cellular material is noticeably higher in sMIC, which is probably due to cell wall damage. sMIC of both EOs have an additive or synergistic effect, reducing MICs, limiting adhesion and preventing the formation of biofilms.


Assuntos
Biofilmes/efeitos dos fármacos , Helichrysum/química , Juniperus/química , Micobactérias não Tuberculosas/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Antibacterianos/farmacologia , Sinergismo Farmacológico , Células HeLa , Humanos , Testes de Sensibilidade Microbiana , Óleos Vegetais/química
4.
Fitoterapia ; 133: 80-84, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30599186

RESUMO

Chemical investigation of aerial parts of Helichrysum italicum yielded two new pyrone derivatives (1 and 2) along with ten known compounds. The structures of the new compounds were established by 1D and 2D NMR spectra as well as by HRESIMS data. Compound 1 represented a rare dimer of substituted α- and γ-pyrone units. DFT-NMR and TDDFT-ECD calculations were carried out to determine the absolute configuration of 1 but failed, representing the limitation of TDDFT-ECD calculation for the configurational assignment. All compounds were measured for their antibacterial and cytotoxic activity but proved to be inactive.


Assuntos
Helichrysum/química , Componentes Aéreos da Planta/química , Pironas/química , Antibacterianos , Itália , Estrutura Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Pironas/isolamento & purificação
5.
Plant Biol (Stuttg) ; 21(3): 498-506, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30120884

RESUMO

Helichrysum microphyllum subsp. tyrrhenicum (Asteraceae) is an endemic taxon of Sardinia and Corsica, where it grows at different altitudes. The objective of this study was to investigate the seed traits and germination behaviour of four Sardinian populations of this taxon located at different altitudes. Seed traits were evaluated, and germination tests were carried out by incubating seeds at a range of constant (5-30 °C) and alternating (25/10 °C) temperatures. The dry after-ripening (DAR) pre-treatment was also applied by storing seed in dry conditions for 3 months at 25 °C. Seed traits and germination behaviour data were statistically analysed to identify if there was a correlation with altitude. Differences in seed size, area and mass among populations were recorded, however, no relationship was found with altitude. High germination percentages were obtained in all populations, both in untreated and DAR seeds, and were positively affected by alternating temperatures. The final germination percentage and time required to reach 50% final germination (T50 ) showed no relationship with altitude. The differences in seed traits and germination detected among the studied populations of H. microphyllum subsp. tyrrhenicum were not correlated with altitude. This study provides new and important knowledge for this taxon. H. microphyllum subsp. tyrrhenicum is characterised by high germination percentages and low T50 values and does not seem to require any dormancy-breaking treatment. This species represents a high-potential native plant species that should be considered within environmental management plans.


Assuntos
Asteraceae/fisiologia , Germinação/fisiologia , Helichrysum/fisiologia , Sementes/fisiologia , Temperatura
6.
Bull Environ Contam Toxicol ; 101(6): 758-765, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30310947

RESUMO

Sardinia was known as an important mine pole in Europe during his history. Still after decades from mine closure, 75.000.000 m3 of mine waste, rich in heavy metals, were left abandoned causing a huge environmental legacy on the mine district area. Consequently, cost effective remediation is required. In this frame, phytoremediation is considered a feasible candidate. This research was focused on Helichrysum microphyllum subsp. tyrrhenicum, which is pioneer in xeric soils with low-functions, like mine tailings. The aim of this study was to evaluate its ability to extract heavy metals from mine soils and accumulate them in plant tissues and its suitability for phytostabilization. Sundry samples of soil, roots and epigean organ were collected through field sampling and analysed in order to obtain metals concentration and mineralogical characteristics. Our results indicate that this species tolerates high concentration of zinc, lead and cadmium, behaving as a species suitable for phytostabilization.


Assuntos
Biodegradação Ambiental , Helichrysum/química , Metais Pesados/análise , Poluentes do Solo/análise , Cádmio/análise , Itália , Raízes de Plantas/química , Solo/química , Zinco/análise
7.
J Appl Microbiol ; 125(4): 1175-1185, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29802669

RESUMO

AIMS: To investigate the microbiological quality of imported fresh leaves on retail sale during 2017 with respect to Salmonella, Shiga-toxin-producing Escherichia coli (STEC) and levels of E. coli. METHODS AND RESULTS: Two hundred and seventy-nine samples of imported edible leaves (69 banana, 77 betel, 118 curry and 15 other types) were tested. Salmonella spp. were confirmed by whole-genome sequencing and isolated from 44 samples, 26% from curry leaves, 14% from betel and 2·4% from all other leaf types: 80% of all samples contained ≥102 , 44% ≥103 and 22% ≥104 CFU of E. coli CFU per g. All samples where Salmonella were detected also yielded ≥20 CFU of E. coli/g. 54 samples were tested for STEC which was detected in six samples and isolated from three: one was identified as STEC O157:H7. CONCLUSIONS: This report further highlights an ongoing problem of Salmonella contamination of imported fresh edible leaves. SIGNIFICANCE AND IMPACT OF THE STUDY: Among all food tested by Public Health England (approximately 11 000 per annum), curry leaves were the herb most commonly contaminated with Salmonella, and betel leaves were the most commonly contaminated ready-to-eat food. The high proportion with unsatisfactory E. coli levels and the detection of STEC suggests risks of contamination by multiple enteric pathogens.


Assuntos
Areca/microbiologia , Helichrysum/microbiologia , Folhas de Planta/microbiologia , Escherichia coli Shiga Toxigênica/crescimento & desenvolvimento , Especiarias/microbiologia , Inglaterra , Contaminação de Alimentos/análise , Contaminação de Alimentos/economia , Salmonella/genética , Salmonella/crescimento & desenvolvimento , Salmonella/isolamento & purificação , Escherichia coli Shiga Toxigênica/genética , Escherichia coli Shiga Toxigênica/isolamento & purificação , Especiarias/economia
8.
Chem Biodivers ; 15(5): e1700545, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29509294

RESUMO

Helichrysum genus consists of about 600 species widespread throughout the world, especially in South Africa and in the Mediterranean area. In this study the aroma profile (HS-SPME) and the EO compositions of seven Helichrysum species (H. cymosum, H. odoratissimum, H. petiolare, H. fontanesii, H. saxatile, H. sanguineum, and H. tenax) were evaluated. All the plants were grown in Italy under the same growth conditions. The volatile constituents, particularly monoterpenes, depended by the plant's genotype and ecological adaptation. This study represents the first headspace evaluation on the selected plants and the results evidenced that monoterpenes represented the main class of constituents in five of the seven species analysed (from 59.2% to 95.0%). The higher content in sesquiterpene hydrocarbons was observed in the Mediterranean species of H. sanguineum (68.0%). Only H. saxatile showed relative similar abundance of monoterpenes and sesquiterpene hydrocarbons. The essential oil composition of the majority of examined species are characterised by high percentage of sesquiterpenes (especially ß-caryophyllene and δ-cadinene) ranging from 51.3% to 92.0%, except for H. cymosum, H. tenax, and H. sanguineum leaves where monoterpenes predominated (from 51.7% to 74.7%).


Assuntos
Helichrysum/química , Óleos Voláteis/química , Helichrysum/crescimento & desenvolvimento , Itália , Óleos Voláteis/isolamento & purificação , Especificidade da Espécie
9.
Molecules ; 23(2)2018 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-29438342

RESUMO

Antennaria dioica (L.) Gaertn. and Helichrysum arenarium (L.) Moench. are two species of the Asteraceae family, known in Romanian traditional medicine for their diuretic, choleretic, and anti-inflammatory properties. The aim of the present study was to evaluate the phenolic and sterolic composition of flowers from the two species and to assess their antioxidant, antibacterial and antifungal properties. LC-MS analyses were performed on methanolic, ethanolic and 70% v/v ethanolic extracts, before and after acid hydrolysis, and revealed high amounts of polyphenols. Chlorogenic acid was found as the main compound for the flowers of A. dioica (502.70 ± 25.11 mg/100 g d.w.), while quercitrin was dominant in H. arenarium (424.28 ± 21.21 mg/100 g d.w.) in 70% v/v ethanolic extracts before hydrolysis. Antioxidant capacity assays showed an important antioxidant potential, which can be correlated with the determined polyphenolic compounds, showing the 70% v/v ethanolic extracts of the two species as being the most effective antioxidant samples for the DPPH assay. Antibacterial and antifungal assays confirm a modest biological potential for the same extract of both species. Results obtained in the present study bring important data and offer scientific evidence on the chemical composition and on the biological activities of the flowers belonging to the two species.


Assuntos
Anti-Infecciosos/química , Antioxidantes/química , Asteraceae/química , Flores/química , Helichrysum/química , Compostos Fitoquímicos/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Aspergillus/efeitos dos fármacos , Aspergillus/crescimento & desenvolvimento , Candida/efeitos dos fármacos , Candida/crescimento & desenvolvimento , Ácido Clorogênico/química , Ácido Clorogênico/isolamento & purificação , Ácido Clorogênico/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Extração Líquido-Líquido/métodos , Penicillium/efeitos dos fármacos , Penicillium/crescimento & desenvolvimento , Fenóis/química , Fenóis/isolamento & purificação , Fenóis/farmacologia , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Fitosteróis/química , Fitosteróis/isolamento & purificação , Fitosteróis/farmacologia , Extratos Vegetais/química , Quercetina/análogos & derivados , Quercetina/química , Quercetina/isolamento & purificação , Quercetina/farmacologia , Solventes/química
10.
Nat Prod Res ; 32(14): 1690-1698, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29098868

RESUMO

Helichrysum faradifani (Asteraceae) is a perennial shrub growing in rocky and sandy places of Madagascar. The plant is used in the Malagasy traditional medicine as a wound-healing agent, disinfectant and for the treatment of syphilis, diarrhea, cough and headache. In the present work, we analysed the chemical composition of the essential oil distilled from the aerial parts of H. faradifani by GC-MS and evaluated its insecticidal activity against 2nd, 3rd and 4th instar larvae of the lymphatic filariasis vector Culex quinquefasciatus by acute toxicity assays. The most sensitive were 2nd instar (LC50 = 85.7 µL L-1) larvae. For the 3rd and 4th instar larvae, the estimated LC50 were 156.8 and 134.1 µL L-1, respectively. Monoterpene hydrocarbons (51.6%) were the major fraction of the essential oil, with the bicyclic α-fenchene (35.6%) as the predominant component. Sesquiterpene hydrocarbons (34.0%) were the second major group characterising the oil, with γ-curcumene (17.7%) as the most abundant component.


Assuntos
Helichrysum/química , Inseticidas/química , Inseticidas/farmacologia , Monoterpenos/análise , Óleos Voláteis/química , Sesquiterpenos/análise , Animais , Culex/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Larva/efeitos dos fármacos , Madagáscar , Monoterpenos/química , Mosquitos Vetores/efeitos dos fármacos , Óleos Voláteis/farmacologia , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Sesquiterpenos/química , Testes de Toxicidade Aguda
11.
Med Arch ; 71(4): 288-292, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28974852

RESUMO

INTRODUCTION: Major advancements in DNA sequencing methods introduced in the first decade of the new millennium initiated a rapid expansion of sequencing studies, which yielded a tremendous amount of DNA sequence data, including whole sequenced genomes of various species, including plants. A set of novel sequencing platforms, often collectively named as "next-generation sequencing" (NGS) completely transformed the life sciences, by allowing extensive throughput, while greatly reducing the necessary time, labor and cost of any sequencing endeavor. PURPOSE: of this paper is to present an overview NGS platforms used to produce the current compendium of published draft genomes of various plants, namely the Roche/454, ABI/SOLiD, and Solexa/Illumina, and to determine the most frequently used platform for the whole genome sequencing of plants in light of genotypization of immortelle plant. MATERIALS AND METHODS: 45 papers were selected (with 47 presented plant genome draft sequences), and utilized sequencing techniques and NGS platforms (Roche/454, ABI/SOLiD and Illumina/Solexa) in selected papers were determined. Subsequently, frequency of usage of each platform or combination of platforms was calculated. RESULTS: Illumina/Solexa platforms are by used either as sole sequencing tool in 40.42% of published genomes, or in combination with other platforms - additional 48.94% of published genomes, followed by Roche/454 platforms, used in combination with traditional Sanger sequencing method (10.64%), and never as a sole tool. ABI/SOLiD was only used in combination with Illumina/Solexa and Roche/454 in 4.25% of publications. CONCLUSIONS: Illumina/Solexa platforms are by far most preferred by researchers, most probably due to most affordable sequencing costs. Taking into consideration the current economic situation in the Balkans region, Illumina Solexa is the best (if not the only) platform choice if the sequencing of immortelle plant (Helichrysium arenarium) is to be performed by the researchers in this region.


Assuntos
Genoma de Planta/genética , Helichrysum , Análise de Sequência de DNA/métodos , Helichrysum/genética , Sequenciamento de Nucleotídeos em Larga Escala , Especificidade da Espécie
12.
J Pharm Biomed Anal ; 145: 593-603, 2017 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-28787672

RESUMO

Several medicinal plants are currently used by the food industry as functional additives, for example botanical extracts in herbal drinks. Moreover, the scientific community has recently begun focusing on halophytes as sources of functional beverages. Helichrysum italicum subsp. picardii (everlasting) is an aromatic halophyte common in southern Europe frequently used as spice and in traditional medicine. In this context, this work explored for the first time H. italicum subsp. picardii as a potential source of innovative herbal beverages with potential health promoting properties. For that purpose, infusions and decoctions were prepared from roots, vegetative aerial-organs (stems and leaves) and flowers and evaluated for in vitro antioxidant and anti-diabetic activities. Samples were also assessed for toxicity in different mammalian cell lines and chemically characterized by spectrophotometric methods and ultra-high performance liquid chromatography-photodiode array-mass-spectrometry (UHPLC-PDA-MS). Results were expressed relating to 'a cup-of-tea' and compared with those obtained with green tea (Camellia sinensis) and rooibos tisane (Aspalathus linearis). Tisanes from the everlasting's above-ground organs, particularly flowers, have high polyphenolic content and several phenolics were identified; the main compounds were chlorogenic and quinic acids, dicaffeoylquinic-acid isomers and gnaphaliin-A. The antioxidant activity of beverages from the everlasting's above-ground organs matched or surpassed that of green tea and rooibos. Its anti-diabetic activity was moderate and toxicity low. Overall, our results suggest that the everlasting is a potential source of innovative and functional herbal beverages.


Assuntos
Aspalathus , Camellia sinensis , Helichrysum , Animais , Linhagem Celular , Europa (Continente) , Extratos Vegetais , Chá , Chás de Ervas
13.
Molecules ; 22(8)2017 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-28771203

RESUMO

Arzanol is a naturally-occurring prenylated acylphloroglucinol isolated from Helichrysum italicum and exhibiting anti-oxidant, antibiotic and antiviral activities. The molecule contains an α-pyrone moiety attached to the phloroglucinol moiety through a methylene bridge. The presence of several hydrogen bond donor or acceptor sites makes intramolecular hydrogen bonding patterns the dominant stabilising factor. Conformers with all the possible different hydrogen bonding patterns were calculated at the HF/6-31G(d,p) and DFT/B3LYP/6-31+G(d,p) levels with fully relaxed geometry in vacuo and in three solvents-chloroform, acetonitrile and water (these levels being chosen to enable comparisons with previous studies on acylphloroglucinols). Calculations in solution were performed with the Polarisable Continuum Model. The results show that the lowest energy conformers have the highest number of stronger intramolecular hydrogen bonds. The influence of intramolecular hydrogen bonding patterns on the other molecular properties is also analysed.


Assuntos
Helichrysum/química , Modelos Moleculares , Floroglucinol/análogos & derivados , Pironas/química , Ligação de Hidrogênio , Estrutura Molecular , Floroglucinol/química
14.
J Zhejiang Univ Sci B ; 18(6): 501-511, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28585426

RESUMO

The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i.p.; Group IV: gentamicin as 80 mg/(kg·d) i.p.; Group V: gentamicin as 80 mg/(kg·d) i.p.+HPE 100 mg/(kg·d) i.p.; and Group VI: gentamicin as 80 mg/(kg·d) i.p.+HPE 200 mg/(kg·d) i.p. for 8 d. Following treatment, serum, liver, and kidney tissues were used to assess blood urea nitrogen (BUN), creatinine, enzymatic and non-enzymatic antioxidants, and lipid peroxidation. Gentamicin significantly increased serum BUN, creatinin, and liver and kidney levels of malondialdehyde (MDA). It also decreased the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin, liver and kidney oxidant marker, and tubular necrosis as well as by an increase in antioxidant enzymes. It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats. As a result, these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters.


Assuntos
Gentamicinas/antagonistas & inibidores , Gentamicinas/toxicidade , Helichrysum , Rim/efeitos dos fármacos , Animais , Antibacterianos/toxicidade , Antioxidantes/farmacologia , Nitrogênio da Ureia Sanguínea , Creatinina/sangue , Etanol , Helichrysum/química , Rim/metabolismo , Rim/patologia , Masculino , Malondialdeído/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Ratos , Ratos Sprague-Dawley , Turquia
15.
Bioorg Med Chem Lett ; 27(12): 2812-2817, 2017 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-28479197

RESUMO

We have successfully established AS model using thoracic aortas vascular ring which evaluated by the morphological changes of blood vessels, the proliferation of VSMC, and the expression of inflammation factors VEGF, CRP, JNK2 and p38. This AS model has the advantages of low cost, convenient and short period of established time. Moreover, we investigated the anti-AS activities of 7 flavonoids Narirutin (1), Naringin (2), Eriodictyol (3), Luteolin (4), Galuteolin (5), Astragalin (6), Kaempferol (7) from flowers of Helichrysum arenarium L. MOENCH by examining the vascular morphology, the inhibition on the expression of inflammation factors CRP, VEGF, JNK2, p38. In addition, we investigated the anti-AS activities of these 7 flavonoids by examining NO secretion of RAW264.7 cells in response to LPS. All above inflammation factors have been proved to be involved in the formation of AS. After comprehensive analysis of all results to discuss the structure-activity relationship, we summarized the conclusions at follow: compounds 1-7 could inhibit the expression of VEGF, CRP, JNK2, p38 and NO at different level, and we evaluated that flavonol aglycone have more significant anti-inflammation than it's glycoside, and the anti-AS activity of flavonols were stronger than flavanones and flavones, which means that 3-group might be the effective group. Eventually, we supposed the main anti-inflammatory mechanism of these compounds was to reduce the expression of CRP, inhibit the kinases activity of JNK2 and p38, and then the MAPK pathway was suppressed, which resulted in the decrease of NO synthesis, VEGF expression and endothelial adhesion factor expression. And eventually, the scar tissue and vascular stenosis formations were prevented. This conclusion suggested flavonoids have the potential of preventing AS formation.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Aterosclerose/tratamento farmacológico , Flavonoides/farmacologia , Flores/química , Helichrysum/química , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Relação Dose-Resposta a Droga , Flavonoides/química , Flavonoides/isolamento & purificação , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Camundongos , Estrutura Molecular , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Células RAW 264.7 , Relação Estrutura-Atividade
16.
Chem Phys Lipids ; 205: 55-64, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28476462

RESUMO

The heterodimeric phloroglucinyl pyrone arzanol (Arz) has raised considerable interest because of its antiviral, anti-inflammatory, and antioxidant activity. We have investigated the effect of methylation of the pyrone moiety on the antioxidant activity and cytotoxicity of Arz. This manoeuvre, that left the polyphenolic moiety unscathed, was nevertheless detrimental for antioxidant activity in both the cholesterol thermal degradation- and the Cu2+-induced liposome oxidation assays, providing evidence of structure-activity relationships that go beyond the preservation of the polyphenolic pharmacophore. The antioxidant activity of Arz was retained also in the Fe-NTA model of in vivo oxidative stress, with protective effect on the oxidative degradation of plasmatic lipids, unsaturated fatty acids and cholesterol. Both Arz and methylarzanol (Me-Arz) were devoid of toxic effect on colonic differentiated Caco-2 cells up to 100µM, but significantly reduced cancer Caco-2 cell viability at lower dosages. Arz could also selectively reduce viability of other cancer cell lines, [murine melanoma cells (B16F10 cells), human cervical carcinoma cells (HeLa cells)], suggesting that it can act as a selective modulator of cell processes typical of cancer cells. Taken together, our results qualify Arz as a lead structure for further in vivo investigation of its pharmacological potential.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Floroglucinol/análogos & derivados , Pironas/química , Pironas/farmacologia , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Colesterol/química , Descoberta de Drogas , Compostos Férricos/química , Helichrysum/química , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Metilação , Ácido Nitrilotriacético/análogos & derivados , Ácido Nitrilotriacético/química , Oxirredução , Estresse Oxidativo , Floroglucinol/química , Floroglucinol/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ratos , Ratos Wistar , Relação Estrutura-Atividade
17.
JAMA Dermatol ; 153(7): 686-688, 2017 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-28445563

RESUMO

Importance: Although Pyemotes species have been known to cause dermatitis, recent reports are rare. During the past 30 years, only 3 outbreaks of dermatitis caused by Pyemotes ventricosus have been reported. Objective: To analyze the causative agent of skin changes in employees of a company that produced herbal medicines. Design, Setting, and Participants: This case series includes 18 patients (8 men, 10 women; mean age, 44 years) who contracted unusual dermatitis after an occupational exposure in July and August of 2012 while working for a company that produced herbal medicines. The patients were examined at the Lower Silesia Regional Centre of Occupational Medicine in Wroclaw, Poland. Exposures: Workers weighed and packed 1 part of the Helichrysum arenarium herb. Main Outcomes and Measures: We hypothesized the causative agent to be P ventricosus. An examination of the herbal specimens for ectoparasites confirmed the diagnosis. Results: Initially 16 employees developed pruritic skin changes. Skin lesions with pruritic vesicles on an erythematous base with surrounding swelling and edema were observed. Several employees also developed a flulike illness. After 44 days, 2 new employees presented with the same skin changes. The analysis of working conditions showed that the same part of the H arenarium herb was weighed and packed at that time. Conclusions and Relevance: We found that changing the time when the herbs were weighed and abandoning gas fumigation containing methyl bromide resulted in the recurrence of an almost forgotten disease.


Assuntos
Dermatite Ocupacional/diagnóstico , Surtos de Doenças , Infestações por Ácaros/diagnóstico , Pele/parasitologia , Adulto , Animais , Dermatite Ocupacional/parasitologia , Feminino , Helichrysum/parasitologia , Humanos , Masculino , Infestações por Ácaros/complicações , Ácaros , Exposição Ocupacional , Polônia , Prurido/parasitologia
18.
Ann Bot ; 119(8): 1365-1383, 2017 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-28334385

RESUMO

Background and Aims: The capitulum of Helichrysum bracteatum is surrounded by scarious involucral bracts that perform hygroscopic movements leading to bract bending toward or away from the capitulum, depending on cell wall water status. The present investigation aimed at explaining the mechanism of these movements. Methods: Surface strain and bract shape changes accompanying the movements were quantified using the replica method. Dissection experiments were used to assess the contribution of different tissues in bract deformation. Cell wall structure and composition were examined with the aid of light and electron microscopy as well as confocal Raman spectroscopy. Key Results: At the bract hinge (organ actuator) longitudinal strains at opposite surfaces differ profoundly. This results in changes of hinge curvature that drive passive displacement of distal bract portions. The distal portions in turn undergo nearly uniform strain on both surfaces and also minute shape changes. The hinge is built of sclerenchyma-like abaxial tissue, parenchyma and adaxial epidermis with thickened outer walls. Cell wall composition is rather uniform but tissue fraction occupied by cell walls, cell wall thickness, compactness and cellulose microfibril orientation change gradually from abaxial to adaxial hinge surface. Dissection experiments show that the presence of part of the hinge tissues is enough for movements. Conclusions: Differential strain at the hinge is due to adaxial-abaxial gradient in structural traits of hinge tissues and cell walls. Thus, the bract hinge of H. bracteatum is a structure comprising gradually changing tissues, from highly resisting to highly active, rather than a bi-layered structure with distinct active and resistance parts, often ascribed for hygroscopically moving organs.


Assuntos
Parede Celular/fisiologia , Helichrysum/fisiologia , Folhas de Planta/fisiologia , Água/fisiologia
19.
Phytochemistry ; 138: 9-28, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28336139

RESUMO

Helichrysum italicum presents a valuable source of natural bioactive compounds. In this work, a literature review of terpenes, phenolic compounds, and other less common phytochemicals from H. italicum with regard to application of different separation methods is presented. Data including extraction/separation methods and experimental conditions applied, obtained yields, number of identified compounds, content of different compound groups, and analytical techniques applied are shown as corresponding tables. Numerous biological activities of both isolates and individual compounds are emphasized. In addition, the data reported are discussed, and the directions for further investigations are proposed.


Assuntos
Helichrysum/química , Fenóis/análise , Compostos Fitoquímicos/análise , Terpenos/análise , Fracionamento Químico/métodos , Óleos Voláteis/análise , Componentes Aéreos da Planta/química , Óleos Vegetais/análise , Solventes/química
20.
Chem Biodivers ; 14(5)2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28122144

RESUMO

Essential oils (EOs) from Schinus molle, Helichrysum gymnocephalum, Cedrelopsis grevei and Melaleuca viridiflora, four aromatic and medicinal plants, are commonly used in folk medicine. EOs were characterized by gas chromatography/mass spectrometry (GC/MS) and quantified by gas chromatography-flame ionization detection (GC-FID); then evaluated for their behavioral effects on adults of the green pea aphid Acyrthosiphon pisum (Harris) using a Perspex four-armed olfactometer in order to test the compatibility of their use as phytoinsecticides to control this insect pest. Our results showed that the EOs from the leaves of S. molle, M. viridiflora and C. grevei did not change aphids' behavior. However, S. molle fruits EO seemed to be attractive while H. gymnocephalum leaves EO exhibited repellency towards aphids at a dose of 10 µl. The major compounds in S. molle fruits EO were 6-epi-shyobunol (16.22%) and d-limonene (15.35%). While, in H. gymnocephalum leaves EO, 1,8-cineole was the main compound (47.4%). The difference in aphids' responses to these two EOs could be attributed to the differences in their compositions. Our findings suggest that these two EOs have potential applications for the integrated pest management of A. pisum (Harris).


Assuntos
Afídeos/efeitos dos fármacos , Inseticidas/isolamento & purificação , Óleos Voláteis/farmacologia , Animais , Helichrysum/química , Hemípteros/efeitos dos fármacos , Inseticidas/farmacologia , Óleos Voláteis/química , Ervilhas , Plantas Medicinais/química
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