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1.
Phytochemistry ; 175: 112372, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32305683

RESUMO

An undescribed iridoid (valeridoid A) and five undescribed bis-iridoids (valeridoids B-F), along with four known ones, were isolated from the roots and rhizomes of Valeriana jatamansi. Their structures were elucidated based on 1D and 2D NMR, as well as HRESIMS spectroscopic data. In addition, 8,9-didehydro-7-hydroxydolichodial and valeridoid F were found to inhibit the growth of three human glioma stem cells (GSC-3#, GSC-12# and GSC-18#).


Assuntos
Nardostachys , Valeriana , Humanos , Iridoides , Estrutura Molecular , Células-Tronco Neoplásicas , Raízes de Plantas
2.
Phytother Res ; 34(6): 1436-1445, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31985131

RESUMO

Stress is an increasing problem that can result in various psychiatric and somatoform symptoms. Among others, benzodiazepines and valerian preparations are used to treat stress symptoms. The aim of this study was to investigate whether the prescription of a fixed herbal extract combination of valerian, lemon balm, passionflower, and butterbur (Ze 185) changes the prescription pattern of benzodiazepines in hospitalized psychiatric patients. In a retrospective case-control study, anonymized medical record data from 3,252 psychiatric in-house patients were analysed over a 3.5-year period. Cases (n = 1,548) with a prescription of Ze 185 and controls (n = 1,704) were matched by age, gender, hospitalization interval, and main International Classification of Diseases, Version 10 F-diagnoses. The primary objective was to investigate the effect of Ze 185 on the prescription pattern of benzodiazepines. Secondary objectives investigated the prescriptions of concomitant drugs and effectiveness of the hospital stay. Distribution of drug classes was analysed using the WHO's anatomic-therapeutic-chemical code. Data showed that both treatment modalities had a comparable clinical effectiveness but with significantly less prescriptions of benzodiazepines in the Ze 185 group (p = .006). This is of clinical importance because suitable alternatives to benzodiazepines are desirable. To obtain more support for this hypothesis, a dedicated randomized, controlled clinical trial monitoring drug safety is required.


Assuntos
Benzodiazepinas/uso terapêutico , Hipnóticos e Sedativos/uso terapêutico , Melissa/química , Passiflora/química , Petasites/química , Extratos Vegetais/uso terapêutico , Valeriana/química , Benzodiazepinas/farmacologia , Estudos de Casos e Controles , Feminino , Humanos , Hipnóticos e Sedativos/farmacologia , Masculino , Pessoa de Meia-Idade , Extratos Vegetais/farmacologia , Estudos Retrospectivos
3.
Plant Foods Hum Nutr ; 75(1): 103-109, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31902039

RESUMO

Adipose tissue is an endocrine organ and its endocrine function is closely associated with type 2 diabetes mellitus. Valeriana officinalis (Valerian) exerts some physiological effects; however, its influence on adipocytes remains unclear. We investigated the effect of methanolic Valerian root extract (Vale) on 3T3-L1 adipocytes. Vale (1, 10, and 100 µg/mL) dose-dependently promoted adipocyte differentiation with increasing lipid accumulation. In addition, Vale significantly increased the mRNA levels in genes associated with adipocyte differentiation, including peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α , and adipocyte protein 2, in dose-dependent manner. Vale also significantly enhanced mRNA and protein levels in adiponectin. A PPARγ antagonist assay and a PPARγ binding assay revealed that Vale-induced increased adipocyte differentiation and adiponectin production were partly associated with direct binding to PPARγ. Valerenic acid, a characteristic component in Valerian, also demonstrated the ability to induce adipocyte differentiation and adiponectin secretion, suggesting that it is one of the functional components in Vale.


Assuntos
Diabetes Mellitus Tipo 2 , Valeriana , Células 3T3-L1 , Adipócitos , Adipogenia , Adiponectina , Animais , Diferenciação Celular , Metanol , Camundongos , PPAR gama , Extratos Vegetais
4.
Planta Med ; 86(3): 172-179, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31801162

RESUMO

Valeriana jatamansi is widely used in Chinese folk medicine and contains iridoids as important active ingredients. The brain-gut axis describes a complex bidirectional system between the central nervous system and the gastrointestinal tract. Herein, we evaluated the antidepressant effects of total iridoids of Valeriana jatamansi (TIV) and preliminarily investigated the effects of gut microbiota on their antidepressant effects using a chronic, unpredictable mild-stress mouse model. Mice were given 5.7, 11.4, or 22.9 mg/kg TIV for 1 week. Fluoxetine (2.6 mg/kg) served as a positive control. Body weight was measured, and behavioral tests including SPT and TST were applied. Colon pathology was assessed through hematoxylin-eosin staining. Additionally, levels of serotonin (5-hydroxytryptamine, 5-HT), norepinephrine (NE), substance P (SP) and corticotropin-releasing factor (CRF) in the hippocampus and colon were measured by ELISA. In addition, 16SrRNA gene sequencing was performed to explore changes in intestinal microbiota richness and diversity. Our results demonstrated that the model group showed significant depression-like behavior, while the fluoxetine group showed improved depression-like symptoms; after administration, TIV increased body weight, sucrose solution consumption, and ameliorated depression-like behaviors. The overall cell degeneration in colons also improved. In addition, TIV modulated the levels of 5-HT, NE, SP, and CRF expression in the hippocampus and colon. The diversity and richness of gut microbes increased compared to the model group. We therefore conclude that the antidepressant effects of TIV may be related to gut flora structures and regulation of 5-HT, NE, SP, and CRF in the brain and intestine.


Assuntos
Trato Gastrointestinal , Valeriana , Animais , Antidepressivos , Comportamento Animal , Encéfalo , Modelos Animais de Doenças , Hipocampo , Intestinos , Iridoides , Camundongos , Estresse Psicológico
5.
Planta Med ; 85(11-12): 925-933, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31127604

RESUMO

A fluorometric imaging plate reader (FLIPR) assay utilizing Chinese hamster ovary (CHO) cells stably transfected with GABAA receptors of α 1 ß 2 γ 2 subunit composition was evaluated and validated for rapid screening of plant extract libraries and efficient localization of active compounds in extracts. Validation was performed with pure compounds and extracts known to contain allosteric GABAA receptor modulators. Plants extracts that had been previously reported as active in an assay using Xenopus laevis oocytes transiently expressing GABAA receptors of α 1 ß 2 γ 2 subunit composition were also active in the FLIPR assay. A protocol for HPLC-based activity profiling was developed, whereby separations of 0.4 - 1.2 mg of extracts on an analytical HPLC column were found to be sufficient for the sensitivity of the bioassay. The protocol successfully localized the activity of known GABAergic natural products, such as magnolol in Magnolia officinalis, valerenic acid in Valeriana officinalis, and piperine in Piper nigrum extract. EC50 values of compounds (magnolol: 4.81 ± 1.0 µM, valerenic acid: 12.56 ± 1.2 µM, and piperine: 5.76 ± 0.7 µM) were found to be comparable or lower than those reported using Xenopus oocyte assays.


Assuntos
Fluorometria/métodos , Extratos Vegetais/farmacologia , Receptores de GABA-A/efeitos dos fármacos , Alcaloides/farmacologia , Animais , Benzodioxóis/farmacologia , Bioensaio/métodos , Compostos de Bifenilo/farmacologia , Células CHO , Cromatografia Líquida de Alta Pressão , Cricetulus , Indenos/farmacologia , Lignanas/farmacologia , Magnolia/química , Oócitos/metabolismo , Piper nigrum/química , Piperidinas/farmacologia , Alcamidas Poli-Insaturadas/farmacologia , Sesquiterpenos/farmacologia , Valeriana/química , Xenopus laevis
6.
J Oleo Sci ; 68(5): 481-491, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31061266

RESUMO

Conjugated linolenic acids (CLNs) are naturally occurring fatty acids that are believed to have anticancer properties. In this study, we examined various plant seeds from herbs to discover seed oils containing CLNs. The ultraviolet spectra of total lipids from these seeds were measured. An absorption maximum around 270 nm was observed in seed oils belonging to the Valerianaceae family (Centranthus ruber and Valeriana officinalis). When the fatty acid compositions of these seed oils were measured, CLNs were detected. By silica column chromatography, neutral lipids (NLs), glycolipids, and phospholipids were eluted from seed oils of C. ruber and V. officinalis. Then, fatty acid compositions of these fractions were measured. This revealed that most of the CLNs in these seed oils existed in the NL fraction. When the NL fractions of these seed oils were reacted with lipase, CLNs showed good sensitivity to lipase hydrolysis. This suggested that the CLNs in the seed oils of C. ruber and V. officinalis existed predominantly at the sn-1,3 position of triacylglycerol and less at the sn-2 position. These results suggested that the CLNs from the seed oils of C. ruber and V. officinalis could easily be taken up by cancer cells as free fatty acids and had good potential as antitumor substances.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacocinética , Ácidos Linoleicos Conjugados/isolamento & purificação , Ácidos Linoleicos Conjugados/farmacologia , Sementes/química , Valeriana/química , Valerianaceae/química , Animais , Camundongos
7.
Environ Sci Pollut Res Int ; 26(16): 16157-16165, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30972667

RESUMO

The essential oil was extracted from the roots of Valeriana officinalis L. by hydrodistillation. The qualitative and quantitative analysis of its chemical constituents was conducted on GC-MS and GC-FID in this study. Seventeen compounds were detected and the major constituents included bornyl acetate (48.2%) and camphene (13.8%). The toxic and repellent effects of the essential oil and its two major constituents were evaluated on Liposcelis bostrychophila and Tribolium castaneum. The results of bioassays indicated that the essential oil showed the promising fumigant and contact toxicity against L. bostrychophila (LC50 = 2.8 mg/L air and LD50 = 50.9 µg/cm2, respectively) and the notable contact effect on T. castaneum (LD50 = 10.0 µg/adult). Meanwhile, the essential oil showed comparable repellent effect on T. castaneum at all testing concentrations. Bornyl acetate and camphene also exhibited strong fumigant and contact toxicity against both species of pests (LC50 = 1.1, 10.1 mg/L air and LD50 = 32.9, 701.3 µg/cm2 for L. bostrychophila; > 126.3, 4.1 mg/L air, and 66.0, 21.6 µg/adult for T. castaneum). Bornyl acetate and camphene showed moderate repellent effect on T. castaneum and conversely showed attractant effect on L. bostrychophila. This work highlights the insecticidal potential of V. officinalis, which has been noted as a traditional medicinal plant.


Assuntos
Canfanos/farmacologia , Repelentes de Insetos/farmacologia , Óleos Voláteis/farmacologia , Terpenos/farmacologia , Valeriana/química , Animais , Monoterpenos Bicíclicos , Armazenamento de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Óleos Voláteis/análise , Óleos Voláteis/química , Ftirápteros/efeitos dos fármacos , Tribolium/efeitos dos fármacos
8.
Anal Bioanal Chem ; 411(14): 3115-3124, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30989266

RESUMO

A novel, sensitive, rapid, and simple fluorescent probe has been developed based on green-synthesized carbon dots (CDs). In this work, CDs have been synthesized from valerian root by hydrothermal method. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) results confirm the formation of CDs with sizes of less than 10 nm. Fluorescence quenching of CDs was due to the aggregation of the negative charges of CDs with the positive charge of imipramine (IMI) and was then used as the signal for determination of IMI. In addition, the cytotoxicity of CDs was determined using the MTT assay. The probe responses under optimum conditions were linear in the range of 1.0-200.0 ng mL-1 with a limit of detection of 0.6 ng mL-1. Afterwards, mesoporous boehmite (MB) was modified with synthesized CDs (CDs/MB). TEM images confirmed MB modification with CDs. In this case, the variations in the fluorescence signal for different concentrations of IMI increased leading to the higher sensitivity for IMI detection. The limit of detection and linear range for determination of IMI with CDs/MB were obtained as 0.2 and 0.5-200.0 ng mL-1, respectively. To evaluate the fluorescent probe, IMI was measured in real samples. Graphical abstract.


Assuntos
Hidróxido de Alumínio/química , Óxido de Alumínio/química , Antidepressivos Tricíclicos/análise , Carbono/química , Corantes Fluorescentes/química , Química Verde , Imipramina/análise , Raízes de Plantas/química , Valeriana/química , Adsorção , Antidepressivos Tricíclicos/sangue , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Imipramina/sangue , Microscopia Eletrônica de Transmissão , Análise Espectral/métodos , Eletricidade Estática , Difração de Raios X
9.
J Asian Nat Prod Res ; 21(9): 842-850, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30729811

RESUMO

Four new monoterpenoids, including two new acyclic monoterpenoids (2R, 6R)-2, 6-dimethyl-8-isovaleroxyoctan-1-ol (1) and (2S, 6S)-2, 6-dimethyl-8-isovaleroxyoctan-1-ol (2), as well as two new iridoids stenopterins F-G (3 and 4), together with fifteen known compounds (5-19), were isolated from whole dried material of Valeriana stenoptera. Stenopterin F was the first reported iridoids with n-butoxyl in the Valerianaceae family. The structures of new compounds were established on the basis of extensive spectroscopic analysis.


Assuntos
Monoterpenos/química , Valeriana/química , Estrutura Molecular
10.
Chem Biodivers ; 16(5): e1800474, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30801931

RESUMO

Twelve 3,8-epoxy iridoids, including four new compounds, jatamanins R-U (1-4), and eight known compounds (5-12), were obtained from the roots and rhizomes of Valeriana jatamansi. The structures were elucidated from analysis of spectroscopic data. The absolute configurations of 1-4 were determined by comparison of experimental and literature ECD spectra. Moreover, the compounds were evaluated for cytotoxic effects against glioma stem cells, inhibition of NO production, activity against influenza A virus and reversal of multidrug resistance of HepG2/ADR cells. Compounds 9 and 12 showed significant cytotoxic potency against GSC-18# (IC50 =1.351 and 4.439 µg ml-1 , respectively) and GSC-3# (IC50 =10.88 and 6.348 µg ml-1 , respectively) glioma stem cells, while compound 12 was also slightly less potent against GSC-12# (IC50 =13.45 µg ml-1 ) glioma stem cell growth. In addition, compounds 9 and 12 displayed obvious inhibition of NO production (IC50 =4.6 and 15.8 µm, respectively).


Assuntos
Iridoides/química , Valeriana/química , Animais , Proliferação de Células/efeitos dos fármacos , Dicroísmo Circular , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Células Hep G2 , Humanos , Iridoides/isolamento & purificação , Iridoides/farmacologia , Lipopolissacarídeos/toxicidade , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Conformação Molecular , Óxido Nítrico/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Células RAW 264.7 , Relação Estrutura-Atividade , Valeriana/metabolismo
11.
Phytomedicine ; 57: 245-254, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30797986

RESUMO

BACKGROUND: F3 is a novel fraction, for the first time isolated from Valeriana jatamansi Jones, which is a traditional Chinese folk medicine. Its anti-cancer potential and the underlying molecular mechanisms have not been well elucidated. PURPOSE: This study aims to investigate the anti-cancer effects of F3 on human breast cancer cell lines and its underlying mechanisms. METHODS: MTT assay was first performed to detect the effect of F3 on cell viability in human breast cancer cell lines and human mammary epithelial MCF-10A cells. Cell apoptosis, mitochondrial membrane potential and ROS level were detected by flow cytometry. Comet and immunofluorescence assays were utilized to assess DNA damage and expression of γ-H2AX. Autophagy were observed by AO staining and fluorescence microscopy. The expression of relative proteins was detected by western blotting. The xenograft model in nude mice was used to elucidate the effect of F3 on tumor growth and DNA damage in vivo. RESULTS: F3 could significantly inhibit the growth of breast cancer cells in concentration-dependent manner by inducing apoptosis and has no obvious inhibitory effect of the growth on MCF-10A cells. Mechanistic studies demonstrated that F3-induced apoptosis was mediated by DNA damage as presented by DNA strand breaks and γ-H2AX activation that might be attacked by ROS accumulation. This triggered several key molecular events involving activation of MAPKs pathway. Further study showed that F3 induced autophagy with the autophagosome formation and increased LC3-II levels. Finally, in vivo study, F3 exhibited a potential antitumor effect and induced DNA damage in MDA-MB-231 xenografts. CONCLUSION: The antitumorigenic activity of F3 was found in vitro and in vivo. These data suggest that F3 may be a potential natural active fraction for the treatment of human breast cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Dano ao DNA/efeitos dos fármacos , Valeriana/química , Animais , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Feminino , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Camundongos Endogâmicos BALB C , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ensaios Antitumorais Modelo de Xenoenxerto
12.
Phytother Res ; 33(4): 939-948, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30632220

RESUMO

Valerian root is the most commonly used herbal supplement for sedation and anxiolysis, but it is unknown whether it may affect functional brain connectivity. Our goal was to use electroencephalography (EEG) to investigate whether valerian root extract (VRE) affects resting-state connectivity changes and whether such changes are associated with clinical symptoms. This 4-week, double-blinded, randomized, placebo-controlled clinical trial was conducted with 64 nonclinical volunteers suffering psychological stress. The participants received VRE (100 mg) or a placebo thrice daily. We examined VRE's therapeutic effects on anxiety and stress-related psychological constructs. Functional brain connectivity changes were measured as EEG coherence in the alpha and theta frequency bands. The VRE and placebo groups both exhibited significant postintervention improvements on all clinical scales, but no significant between-group differences in these changes were noted. Compared with the placebo group, the VRE group exhibited significantly greater increases in frontal brain region alpha coherence across four electrode pairs, and these changes were significantly correlated with anxiolysis. The VRE group also exhibited significantly greater decreases in theta coherence across another four electrodes pairs. Our findings indicate that VRE alters functional brain connectivity in relation to anxiety. Further EEG studies are needed to confirm VRE's neurophysiological effects.


Assuntos
Ansiolíticos/farmacologia , Encéfalo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Valeriana , Adulto , Encéfalo/fisiologia , Método Duplo-Cego , Eletroencefalografia/efeitos dos fármacos , Feminino , Humanos , Hipnóticos e Sedativos/farmacologia , Masculino , Pessoa de Meia-Idade , Estresse Fisiológico/efeitos dos fármacos , Valeriana/química
13.
Phytother Res ; 33(3): 482-503, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30663144

RESUMO

Valeriana jatamansi Jones (Family: Caprifoliaceae), a high value medicinal plant, was distributed in many countries of Asia. The species possesses important valepotriates and is a good source of flavones or flavone glycosides, lignans, sesquiterpenoids or sesquiterpenoid glycoside, bakkenolide type sesquiterpenoids, phenolic compounds, terpinoids, etc. The use of the species in traditional and modern medicines is well known. For instance, V. jatamansi is very important for its insect repelling and antihelmethic properties. Similarly, sedative, neurotoxic, cytotoxic, antidepressant, antioxidant, and antimicrobial activities of the species in various ailments in the indigenous system of medicine, particularly in Asia, are reported. This review focuses on the detailed phytochemical composition, medicinal uses, and pharmacological properties of V. jatamansi along with analysis of botanical errors in published literature and reproducibility of the biomedical researches on this multipurpose herbaceous species.


Assuntos
Extratos Vegetais/uso terapêutico , Plantas Medicinais , Valeriana/química , Antioxidantes/química , Humanos , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/uso terapêutico , Fitoterapia/métodos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Reprodutibilidade dos Testes
14.
BMC Nephrol ; 19(1): 379, 2018 12 27.
Artigo em Inglês | MEDLINE | ID: mdl-30591031

RESUMO

BACKGROUND: The prevalence of cognitive disorders in hemodialysis patients is twice as high as the general population, while these disorders often are undiagnosed. Timely prevention and treatment can improve their personal and social functions. Aim of study was determined the effect of Valerian on cognitive disorders and electroencephalography (EGG) in hemodialysis patients. METHODS: This crossover, double-blind clinical trial was conducted on 39 hemodialysis patients. The patients were randomly divided into two groups. Group A (n = 19) took Valerian capsules and Group B (n = 20) received placebo capsules 60 min before bedtime for one month. The type of treatment was replaced between the two groups after a one-month wash-out. The Mini Mental State Examination (MMSE) questionnaire was completed and EGG was performed before and after intervention in both periods. RESULTS: The cognitive scores of the Group valerian were increased significantly in the first (p = 0.003) and the second (p = 0.005) periods. In addition, the mean increase in the cognitive scores in the Group valerian was significant in the first (p = 0.028) and the second periods (p = 0.030). However, the changes in EGG showed no significant difference before and after intervention in two groups. CONCLUSION: The findings of this study indicated that valerian could be effective and significantly improve patients' cognitive status; however, no significant changes were observed in the electroencephalography of the hemodialysis patients. TRIAL REGISTRATION: IRCT201606076318N7 -2016-06-17.


Assuntos
Transtornos Cognitivos/tratamento farmacológico , Eletroencefalografia/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/uso terapêutico , Valeriana , Adulto , Idoso , Idoso de 80 Anos ou mais , Transtornos Cognitivos/etiologia , Estudos Cross-Over , Método Duplo-Cego , Feminino , Humanos , Masculino , Testes de Estado Mental e Demência , Pessoa de Meia-Idade , Extratos Vegetais/farmacologia , Diálise Renal/efeitos adversos , Insuficiência Renal Crônica/terapia
15.
Rev Med Suisse ; 14(626): 2018-2023, 2018 Nov 07.
Artigo em Francês | MEDLINE | ID: mdl-30422422

RESUMO

Sleep disorders are a recurrent complaint in geriatrics. Of multifactorial origin, they have a significant impact on health and quality of life. However, the answer is (too) often the prescription of benzodiazepines or related-drugs (Z-pills), sedative antidepressant, or another psychotropic medication. More recently, melatonin, valerian and, in Switzerland, clomethiazol are widely considered as effective and more suitable alternatives for aged people. We present a systematic review of the literature on the efficacy and tolerance of these molecules, of which the main objective is to demonstrate that non-pharmacological approach must remain the first-line therapy of insomnia in geriatrics.


Assuntos
Hipnóticos e Sedativos , Melatonina , Distúrbios do Início e da Manutenção do Sono , Valeriana , Benzodiazepinas/uso terapêutico , Clormetiazol/uso terapêutico , Humanos , Hipnóticos e Sedativos/uso terapêutico , Melatonina/uso terapêutico , Qualidade de Vida , Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico , Suíça
16.
Fitoterapia ; 131: 80-85, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30339922

RESUMO

Two new iridoids (1-2) and a new decomposition product of valepotriates (3), together with fifteen known compounds (4-18) were isolated from the roots and rhizomes of Valeriana polystachya Smith, a native species from the Pampa Biome. Their structures were elucidated by means of NMR spectroscopy, mass spectrometry and optical rotation. The structures of 3 and 18 were further confirmed by single crystal X-ray diffraction analysis. In the group of the isolated compounds, 6ß-hydroxysitostenone, hydroxymaltol and isovillosol were isolated from the Valeriana genus for the first time. The extracts and isolated compounds were evaluated for their in vitro activities against acetylcholinesterase (AChE) and prolyloligopeptidase (POP). Compounds 7, 9 and 11 showed weak inhibitory activity against AChE, while 3 and 5 displayed exceptional POP inhibitory activity, with IC50 values of 5.3 ±â€¯0.07 and 7.9 ±â€¯0.4 µM, respectively.


Assuntos
Inibidores da Colinesterase/isolamento & purificação , Iridoides/isolamento & purificação , Inibidores de Serino Proteinase/isolamento & purificação , Valeriana/química , Acetilcolinesterase , Brasil , Inibidores da Colinesterase/farmacologia , Iridoides/farmacologia , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Raízes de Plantas/química , Rizoma/química , Serina Endopeptidases , Inibidores de Serino Proteinase/farmacologia
17.
Molecules ; 23(9)2018 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-30213112

RESUMO

Zhi zhu xiang (ZZX for short) is the root and rhizome of Valeriana jatamansi Jones, which is a Traditional Chinese Medicine (TCM) used to treat various mood disorders for more than 2000 years, especially anxiety. The aim of the present work was to identify the bioactive chemical markers in Zhi zhu xiang improving anxiety in rats by a fingerprint-efficacy study. More specifically, the chemical fingerprint of ZZX samples collected from 10 different regions was determined by High Performance Liquid Chromatography (HPLC) and the similarity analyses were calculated based on 10 common characteristic peaks. The anti-anxiety effect of ZZX on empty bottle stimulated rats was examined through the Open Field Test (OFT) and the Elevated Plus Maze Test (EPM). Then we measured the concentration of CRF, ACTH, and CORT in rat's plasma by the enzyme-linked immune sorbent assay (ELISA) kit, while the concentration of monoamine and metabolites (NE, DA, DOPAC, HVA, 5-HT, 5-HIAA) in the rat's cerebral cortex and hippocampus was analysed by HPLC coupled with an Electrochemical Detector. At last, the fingerprint-efficacy study between chemical fingerprint and anti-anxiety effect of ZZX was accomplished by partial least squares regression (PLSR). As a result, we screened out four compounds (hesperidin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) as the bioactive chemical markers for the anti-anxiety effect of ZZX. The fingerprint-efficacy study we established might provide a feasible way and some elicitation for the identification of the bioactive chemical markers for TCM.


Assuntos
Ansiedade/tratamento farmacológico , Ácido Clorogênico/administração & dosagem , Medicamentos de Ervas Chinesas/administração & dosagem , Hesperidina/administração & dosagem , Valeriana/química , Hormônio Adrenocorticotrópico/sangue , Animais , Ansiedade/sangue , Ansiedade/etiologia , Ácido Clorogênico/análogos & derivados , Ácido Clorogênico/química , Ácido Clorogênico/farmacologia , Cromatografia Líquida de Alta Pressão , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Hesperidina/química , Hesperidina/farmacologia , Análise dos Mínimos Quadrados , Masculino , Neuropeptídeos/sangue , Raízes de Plantas/química , Ratos , Receptores de Hormônio Liberador da Corticotropina/sangue , Rizoma/química
18.
Fitoterapia ; 130: 73-78, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30138654

RESUMO

Four new iridoids (1-4), together with three known iridoids (5-7), one known flavonoid glycoside, three phenolic acids and one phytosterol were isolated from the roots of Valeriana dioscoridis. Their structures were elucidated by means of NMR spectroscopy and mass spectrometry. This is the first report on the phytochemical composition of the non-volatile constituents of V. dioscoridis and the occurrence of a bis-iridoid glycoside in the genus Valeriana. The antiproliferative effects of the iridoids (1-7) were evaluated against three human cancer cell lines of gynacological origin (HeLa, A2780 and T47D) at 10, 30 and 60 µM concentrations, using the MTT assay and they elicited modest antiproliferative activity when compared to the reference agent, cisplatin.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Iridoides/isolamento & purificação , Valeriana/química , Antineoplásicos Fitogênicos/farmacologia , Células HeLa , Humanos , Iridoides/farmacologia , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Raízes de Plantas/química , Plantas Medicinais/química , Turquia
19.
Int J Pharm Compd ; 22(3): 240-246, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29878891

RESUMO

The physical, chemical, and microbiological stability of a compounded oral solution with the active ingredients herbal tinctures of valerian and motherwort with sedative action for pediatric treatment was studied. Evaluations for physical, chemical, and microbiological stability were performed initially and throughout the storage period. Physical stability of the oral solution was assessed by coloration, clarity, and pH of the solution. The physical appearance of the oral solution did not change throughout the study period. The chemical stability of the oral solution was evaluated by means of a stability-indicating high-performance thin-layer chromatography analytical technique, identification tests, and assay method of sodium bromide. The microbiological stability of the oral solution was investigated by using the European Pharmacopoeia method using the acceptance criteria for nonsterile aqueous preparations for oral use. It was found that the compounded oral solution was stable for at least 21 days at 25°C ± 2°C/60% RH and 5°C ± 3°C, when protected from light.


Assuntos
Bactérias/isolamento & purificação , Brometos/química , Contaminação de Medicamentos , Fungos/isolamento & purificação , Soluções Farmacêuticas/química , Extratos Vegetais/química , Preparações de Plantas/química , Compostos de Sódio/química , Administração Oral , Cromatografia Líquida de Alta Pressão , Composição de Medicamentos , Estabilidade de Medicamentos , Humanos , Leonurus , Valeriana
20.
Biomed Pharmacother ; 99: 913-920, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29710491

RESUMO

The aim of this study was to investigate the beneficial effect of Valerian/Cascade mixture on sleeping in mammal models. In pentobarbital-induced sleep model, Valerian, Cascade, and Valerian/Cascade mixture significantly reduced the latency time for sleeping, and total sleeping time effectively increased in these sample groups compared with the control. Valerian/Cascade mixture increased sleep duration by 37%. The mixture significantly increased the non-rapid eye movement (NREM) sleep time by 53% compared with the control, while REM sleeping time was decreased by 33% with Valerian/Cascade mixture, in Electroencephalography (EEG) analysis, resulting in the increase of total sleep time and the decrease of awakening. This sleep-promoting effect was obvious in caffeine-induced awakening model; Valerian, Cascade, and the mixture significantly enhanced NREM and total sleep time, which were reduced by caffeine. Caffeine-induced increase of awakening was effectively deceased to the normal level by these three samples. In particular, delta wave responsible for deep sleep in NREM was greatly increased by the mixture in both normal and caffeine-induced awake models. This sleep-promoting effect of Valerian/Cascade mixture was shown to be due to the upregulation of gamma-aminobutyric acid A receptor (GABAAR). Valerian/Cascade mixture showed 91% binding capacity to GABAA-BZD receptor. Two compounds, Valerenic acid and Xanthohumol, were shown to significantly contribute to the binding activity of Valerian/Cascade mixture on the GABA receptor.


Assuntos
Humulus/química , Extratos Vegetais/farmacologia , Sono/efeitos dos fármacos , Valeriana/química , Animais , Cafeína/farmacologia , Eletroencefalografia , Hipnóticos e Sedativos/administração & dosagem , Hipnóticos e Sedativos/isolamento & purificação , Hipnóticos e Sedativos/farmacologia , Camundongos , Camundongos Endogâmicos ICR , Extratos Vegetais/administração & dosagem , Ratos , Ratos Sprague-Dawley , Receptores de GABA-A/efeitos dos fármacos , Receptores de GABA-A/metabolismo , Fases do Sono/efeitos dos fármacos , Sono REM/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacos
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