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1.
J Oleo Sci ; 69(4): 317-326, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32249260

RESUMO

Seed extracts of Nymphia alba Linn. and Lupinus polyphyllus Lindl. were analyzed for fatty acid composition, functional group analysis and antioxidant activity. The petroleum ether extract of seeds were found dominant in unsaturated fatty acids with oleic acid (39.9%) and linoleic acid (29.6%) in L. polyphyllus and linoleic (37.5%) and oleic acid (10.9%) in N. alba. All the defatted seed extracts of N. alba and L. polyphyllus found to have powerful DPPH, ABTS, H2O2 and NBT antioxidant radical scavenging activity with reference to butylated hydroxy toluene (BHT). The defatted seed extracts were further analyzed with functional group analysis through FTIR found to contain numerous functional groups which may be responsible for their antioxidant activity.


Assuntos
Antioxidantes , Ácidos Graxos/análise , Lupinus/química , Nymphaea/química , Extratos Vegetais/química , Sementes/química , Depuradores de Radicais Livres , Cromatografia Gasosa-Espectrometria de Massas , Ácido Linoleico/análise , Ácido Oleico/análise
2.
Int J Mol Sci ; 21(7)2020 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-32276381

RESUMO

Narrow-leafed lupin (Lupinus angustifolius L.) has recently been supplied with advanced genomic resources and, as such, has become a well-known model for molecular evolutionary studies within the legume family-a group of plants able to fix nitrogen from the atmosphere. The phylogenetic position of lupins in Papilionoideae and their evolutionary distance to other higher plants facilitates the use of this model species to improve our knowledge on genes involved in nitrogen assimilation and primary metabolism, providing novel contributions to our understanding of the evolutionary history of legumes. In this study, we present a complex characterization of two narrow-leafed lupin gene families-glutamine synthetase (GS) and phosphoenolpyruvate carboxylase (PEPC). We combine a comparative analysis of gene structures and a synteny-based approach with phylogenetic reconstruction and reconciliation of the gene family and species history in order to examine events underlying the extant diversity of both families. Employing the available evidence, we show the impact of duplications on the initial complement of the analyzed gene families within the genistoid clade and posit that the function of duplicates has been largely retained. In terms of a broader perspective, our results concerning GS and PEPC gene families corroborate earlier findings pointing to key whole genome duplication/triplication event(s) affecting the genistoid lineage.


Assuntos
Genoma de Planta , Glutamato-Amônia Ligase/genética , Lupinus/genética , Fosfoenolpiruvato Carboxilase/genética , Duplicações Segmentares Genômicas , Evolução Molecular , Lupinus/metabolismo , Nitrogênio/metabolismo , Análise de Sequência de DNA , Sintenia
3.
Food Chem ; 324: 126878, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32344348

RESUMO

Ceramides (Cer) and cerebrosides are important sphingolipids (SL) involved in many biological processes. Herein, the SL content of yellow lupin seeds (Lupinus luteus) was determined by liquid-liquid extraction, mild alkaline hydrolysis (1 h at 37 °C) and reversed-phase liquid chromatography with negative electrospray ionization coupled to either an orbital-trap (Fourier-transformed, FT) or linear ion-trap (LIT) mass spectrometry (RPLC-ESI/MS). The chemical identity of SL including the sugar residues and sphingoid backbone (SB) was inferred by collision-induced dissociation multiple-stage mass spectrometry (MSn, n = 2,3). Up to 52 Cer and 47 cerebrosides were successfully recognized and quantified in sample extracts of L. luteus seeds also counting isobaric species. As reported for other vegetables, a hydroxylated SB was observed whereby the N-acyl chains showed a high occurrence of very-long-chain moieties; phytosphingosine and 4-hydroxy-8-sphingenine were the predominant SB paired mainly with oleic acid (C18:1), hydroxylated behenic acid (C22:0;1) and hydroxylated lignoceric acid (C24:0;1).


Assuntos
Ceramidas/análise , Cerebrosídeos/análise , Lupinus/química , Sementes/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Ácidos Graxos/análise , Ácidos Graxos/química , Lupinus/metabolismo , Sementes/metabolismo , Esfingosina/análogos & derivados , Esfingosina/análise
4.
Nat Commun ; 11(1): 1069, 2020 02 26.
Artigo em Inglês | MEDLINE | ID: mdl-32103018

RESUMO

White lupin (Lupinus albus) is a legume crop that develops cluster roots and has high phosphorus (P)-use efficiency (PUE) in low-P soils. Here, we assemble the genome of white lupin and find that it has evolved from a whole-genome triplication (WGT) event. We then decipher its diploid ancestral genome and reconstruct the three sub-genomes. Based on the results, we further reveal the sub-genome dominance and the genic expression of the different sub-genomes varying in relation to their transposable element (TE) density. The PUE genes in white lupin have been expanded through WGT as well as tandem and dispersed duplications. Furthermore, we characterize four main pathways for high PUE, which include carbon fixation, cluster root formation, soil-P remobilization, and cellular-P reuse. Among these, auxin modulation may be important for cluster root formation through involvement of potential genes LaABCG36s and LaABCG37s. These findings provide insights into the genome evolution and low-P adaptation of white lupin.


Assuntos
Adaptação Fisiológica/genética , Cromossomos de Plantas/genética , Genoma de Planta/genética , Lupinus/genética , Fósforo/metabolismo , Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética , Elementos de DNA Transponíveis/genética , Evolução Molecular , Regulação da Expressão Gênica de Plantas/genética , Proteínas de Plantas/genética , Raízes de Plantas/metabolismo
5.
Plant Foods Hum Nutr ; 75(2): 215-222, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32086676

RESUMO

Cardiovascular disease (CVD) is the leading cause of death throughout the world. A major risk factor for CVD is platelet aggregation. Various plant extracts exhibit anti-aggregatory action in vitro. The dietary intake of traditional plant crops such as quinoa (Chenopodium quinoa Willd) and lupin (Lupinus spp., Fabaceae family), highly recognized for their high nutritional value, is increasing worldwide. The aim of the study was to assay possible antiplatelet effects of quinoa and lupin bean extracts in vitro. The proximate chemical composition of quinoa grains and the three most widely known lupin cultivars: blue (L. angustifolius), yellow (L. luteus or mutabilis) and white (L. albus) grown in Chile were analyzed. The anti-aggregation activity of the ethanol extracts of the crops was assayed using flow cytometry in ADP-stimulated human platelets, and their inhibition of the maximal platelet aggregation was measured. All the lupin extracts exhibited a significant anti-aggregatory effect (p < 0.0001), while quinoa extracts did not exert this effect compared to control platelets. In conclusion, lupin beans extracts exhibited an anti-aggregatory effect on activated human platelets.


Assuntos
Chenopodium quinoa , Lupinus , Chile , Humanos , Extratos Vegetais , Agregação Plaquetária , Sementes
6.
Plant Foods Hum Nutr ; 75(2): 184-191, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32009208

RESUMO

The evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. Debittering of lupin seeds has a long history; however, the control of the level of alkaloids after processing the seeds is typically only evaluated by changes in the bitter taste. The aim of this study was to evaluate the profile and residual levels of quinolizidine alkaloids (QA) in (Lupinus mutabilis Sweet) after aqueous debittering process. Samples from 10 ecotypes from different areas of Peru were analyzed before and after the process. Based on results obtained by gas chromatography and mass spectrometry, from eight alkaloids identified before the debittering process, only small amounts of lupanine (avg. 0.0012 g/100 g DM) and sparteine (avg. 0.0014 g/100 g DM) remained in the seeds after the debittering process, and no other alkaloids were identified. The aqueous debittering process reduced the content of alkaloids to levels far below the maximal level allowed by international regulations (≤ 0.2 g/kg DM).


Assuntos
Alcaloides , Lupinus , Ecótipo , Cromatografia Gasosa-Espectrometria de Massas , Sementes , Paladar
7.
Food Chem ; 315: 126238, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32000081

RESUMO

The effects of solid-state fermentation (SSF) with Aspergillus sojae, Aspergillus ficuum and their co-cultures on proximate composition, anti-nutritional factor, microbiological and functional properties of lupin flour (LF) were investigated. Fibre fractions, in vitro enzyme protein digestion (IVPD), total phenolic contents, protein molecular distribution and colour attributes were also evaluated. Samples differed in their proximate composition except ash and fibre contents. The microbiological counts of the fermented LFs were generally higher (p < 0.05) than that of the unfermented LF. Phytic acid content and IVPD decreased (p < 0.05) in the fermented LFs. Also, the fermented LFs showed decreased (p < 0.05) water absorption capacity but increased swelling capacity. In addition, fermented LFs demonstrated reduction in colour attributes. Thus, the study indicated that SSF using Aspergillus sojae and Aspergillus ficuum can influence the physical, chemical and functional properties of LF. LF has great potentials in developing new nutritious food products and feed formulations when subjected to SSF.


Assuntos
Farinha/análise , Farinha/microbiologia , Lupinus/metabolismo , Aspergillus , Fibras na Dieta/análise , Proteínas na Dieta/análise , Proteínas na Dieta/metabolismo , Fermentação , Lupinus/química , Fenóis/análise , Ácido Fítico/análise
8.
Nat Commun ; 11(1): 492, 2020 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-31980615

RESUMO

White lupin (Lupinus albus L.) is an annual crop cultivated for its protein-rich seeds. It is adapted to poor soils due to the production of cluster roots, which are made of dozens of determinate lateral roots that drastically improve soil exploration and nutrient acquisition (mostly phosphate). Using long-read sequencing technologies, we provide a high-quality genome sequence of a cultivated accession of white lupin (2n = 50, 451 Mb), as well as de novo assemblies of a landrace and a wild relative. We describe a modern accession displaying increased soil exploration capacity through early establishment of lateral and cluster roots. We also show how seed quality may have been impacted by domestication in term of protein profiles and alkaloid content. The availability of a high-quality genome assembly together with companion genomic and transcriptomic resources will enable the development of modern breeding strategies to increase and stabilize white lupin yield.


Assuntos
Genoma de Planta , Lupinus/genética , Sementes/fisiologia , Análise de Sequência de DNA , Solo , Alcaloides/química , Alcaloides/metabolismo , Centrômero/genética , Ecótipo , Evolução Molecular , Dosagem de Genes , Duplicação Gênica , Variação Genética , Variação Estrutural do Genoma , Lupinus/crescimento & desenvolvimento , Modelos Genéticos , Anotação de Sequência Molecular , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Polimorfismo de Nucleotídeo Único/genética , Sequências Repetitivas de Ácido Nucleico/genética , Sintenia/genética , Transcriptoma/genética
9.
Toxicon ; 176: 21-29, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31965970

RESUMO

Larkspurs, lupines, and death camas can be acutely toxic to livestock and are serious poisonous plant problems in western North America. The toxicity of these plants depends on the composition and concentrations of the toxic alkaloids in the plants. In this study, goats and cows were dosed sub-lethal doses of larkspur, lupine, and death camas. Rumen contents and ocular fluid samples were collected, and simple extraction, sample preparation, and analytical methods were developed for the detection of toxic alkaloids in the rumen contents and ocular fluid samples. Toxic alkaloids were detected in the rumen contents and ocular fluid samples from the goats and cows dosed larkspur, lupine, and death camas. In addition, results from a case report where rumen contents were analyzed from a steer that was suspected to have died due to larkspur are reported. This demonstrates the utility of the methods described for the diagnosis of acute plant poisonings.


Assuntos
Alcaloides/toxicidade , Delphinium , Lupinus , Plantas Tóxicas/toxicidade , Rúmen , Zigadenus , Animais , Bovinos , Olho/química , Cabras , Intoxicação por Plantas/veterinária
10.
Plant Biol (Stuttg) ; 22(2): 298-308, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31758626

RESUMO

There is growing interest in harnessing the genetic and adaptive diversity of crop wild relatives to improve drought resilience of elite cultivars. Rainfall gradients exert strong selection pressure on both natural and agricultural ecosystems. Understanding plant responses to these facilitates crop improvement. Wild and domesticated narrow-leafed lupin (NLL) collected along Mediterranean terminal drought stress gradients was evaluated under contrasting reproductive phase water supply in controlled field, glasshouse and cabinet studies. Plant phenology, growth and productivity, water use and stress responses were measured over time. There is an integrated suite of adaptive changes along rainfall gradients in NLL. Low rainfall ecotypes flower earlier, accumulate lower seed numbers, biomass and leaf area, and have larger root:shoot ratios than high rainfall ecotypes. Water-use is lower and stress onset slower in low compared to high rainfall ecotypes. Water-use rates and ecotypic differences in stress response (Ψleaf decline, leaf loss) are lower in NLL than yellow lupin (YL). To mitigate the effects of profligate water use, high rainfall YL ecotypes maintain higher leaf water content over declining leaf water potential than low rainfall ecotypes. There is no evidence for such specific adaptation in NLL. The data suggests that appropriate phenology is the key adaptive trait to rainfall gradients in NLL because of the flow-on effects on biomass production, fitness, transpiration and stress onset, and the lack of physiological adaptations as in YL. Accordingly, it is essential to match phenology with target environment in order to minimize risk and maximize yield potential.


Assuntos
Adaptação Fisiológica , Lupinus , Água , Secas , Ecossistema , Ecótipo , Lupinus/fisiologia , Região do Mediterrâneo , Chuva , Água/metabolismo
11.
Arch Microbiol ; 202(2): 283-291, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31650197

RESUMO

Metabolic changes occurring in white lupine grain were investigated in response to Plant Growth Promoting Rhizobacteria (PGPR) root inoculation under field condition. We precisely targeted lipids and phenolics changes occurring in white lupine grain in response to Pseudomonas brenneri LJ215 and/or Paenibacillus glycanilyticus LJ121 inoculation. Lipids and phenolic composition were analyzed using an Ultra High-Performance Liquid Chromatography/Tandem Mass Spectrometry Methods. As compared to grain of un-inoculated control plant, Paenibacillus glycaniliticus inoculation highly increased the total lipids content (from 232.55 in seeds of un-inoculated control plant to 944.95 mg/kg) and the relative percentage of several fatty acid such as oleic acid (+20.95%) and linoleic acid (+14.28%) and decreased the relative percentage of glycerophospholipids (- 13.11%), sterol (- 0.2% and - 0.34% for stigmasterol and campesterol, respectively) and prenol (- 17.45%) class. Paenibacillus glycaniliticus inoculation did not affect total phenolic content, while it modulated content of individual phenolic compounds and induced the accumulation of "new" phenolics compounds such as kaempferol. Paenibacillus glycanilyticus LJ121 can be a useful bio-fertilizer to enhance nutritional quality of white lupine grain.


Assuntos
Lupinus/microbiologia , Valor Nutritivo , Paenibacillus/fisiologia , Sementes/química , Endófitos/fisiologia , Lupinus/química , Fenóis/análise , Raízes de Plantas/microbiologia , Pseudomonas/crescimento & desenvolvimento , Sementes/microbiologia
12.
Braz J Biol ; 80(1): 23-29, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31017230

RESUMO

Most of the wild and native legume seeds has a hard and impermeable testa, which causes physical dormancy and prevents them from germinating even when environmental conditions are favorable. The study evaluated the effect of scarification treatments on germination and enzymatic activity of Crotalaria longirostrata (Cl) and Lupinus exaltatus (Le) seeds. After scarification treatments, germination percentage (GP) and rate (GR) were assessed during 30 days after seeding (DAS); and water absorption (WA) and specific enzymatic activity (SEA) during early germination (0, 6, 18, 36, 72, 120 h) in a growing chamber at 25 °C and photoperiod of 12 h. Scarification with 98% H2SO4 15 min increased GP and GR in both species. At 30 DAS, GP and GR of Le seeds were 34% and 0.97 seeds day-1, respectively. In Cl seeds, GP was 64% and GR 0.90 seeds day-1. Scarification with H2O at 80 °C 1 min also promoted germination in Cl (52%). At 120 h after seeding, Le and Cl seeds showed already a high GP with acid scarification (31% and 48%, respectively). In seeds of both species, scarification treatments affected WA and SEA during early germination. During this period, scarification treatments that increased GP also showed a higher α-D-galactosidase activity. The maximum enzyme activity was observed 72 h after hot water scarification in Cl (82.6 U/mg total protein), followed by acid scarification (54.5 U/mg total protein). In Le, the activity peak was 36 h after acid scarification (9.5 U/mg total protein). No relationship was observed between ß-glucosidase activity and GP in both species. In conclusion, during early germination of both species, the increase in GP is accompanied by a rise in α-D-galactosidase activity between 36 and 72 h after seeding; and in Cl seeds, an alternative scarification treatment to increase GP may be the use of hot water.


Assuntos
Crotalaria , Fabaceae , Lupinus , Germinação , Sementes , Água
13.
Int J Mol Sci ; 20(22)2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31726789

RESUMO

The last century has witnessed rapid domestication of the narrow-leafed lupin (Lupinus angustifolius L.) as a grain legume crop, exploiting discovered alleles conferring low-alkaloid content (iucundus), vernalization independence (Ku and Julius), and reduced pod shattering (lentus and tardus). In this study, a L. angustifolius mapping population was subjected to massive analysis of cDNA ends (MACE). The MACE yielded 4185 single nucleotide polymorphism (SNP) markers for linkage map improvement and 30,595 transcriptomic profiles for expression quantitative trait loci (eQTL) mapping. The eQTL highlighted a high number of cis- and trans-regulated alkaloid biosynthesis genes with gene expression orchestrated by a regulatory agent localized at iucundus locus, supporting the concept that ETHYLENE RESPONSIVE TRANSCRIPTION FACTOR RAP2-7 may control low-alkaloid phenotype. The analysis of Ku shed light on the vernalization response via FLOWERING LOCUS T and FD regulon in L. angustifolius, providing transcriptomic evidence for the contribution of several genes acting in C-repeat binding factor (CBF) cold responsiveness and in UDP-glycosyltransferases pathways. Research on lentus selected a DUF1218 domain protein as a candidate gene controlling the orientation of the sclerified endocarp and a homolog of DETOXIFICATION14 for purplish hue of young pods. An ABCG transporter was identified as a hypothetical contributor to sclerenchyma fortification underlying tardus phenotype.


Assuntos
Mapeamento Cromossômico , Domesticação , Regulação da Expressão Gênica de Plantas/fisiologia , Genes de Plantas , Lupinus , Folhas de Planta , Proteínas de Plantas , Locos de Características Quantitativas , Lupinus/genética , Lupinus/metabolismo , Folhas de Planta/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/biossíntese , Proteínas de Plantas/genética
14.
Opt Express ; 27(20): 28640-28648, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684612

RESUMO

Here, we report on a new record in the acquisition time for fast neutron tomography. With an optimized imaging setup, it was possible to acquire single radiographic projection images with 10 ms and full tomographies with 155 projections images and a physical spatial resolution of 200 µm within 1.5 s. This is about 6.7 times faster than the current record. We used the technique to investigate the water infiltration in the soil with a living lupine root system. The fast imaging setup will be part of the future NeXT instrument at ILL in Grenoble with a great field of possible future applications.


Assuntos
Nêutrons , Tomografia , Lupinus/fisiologia , Raízes de Plantas/fisiologia , Intensificação de Imagem Radiográfica , Solo
15.
Int J Mol Sci ; 20(22)2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31698683

RESUMO

The quality and amount of yellow lupine yield depend on water availability. Water scarcity negatively affects germination, flowering, and pod formation, and thus introduction of an artificial irrigation system is needed. The aim of this study was to evaluate the influence of irrigation on the quality of yellow lupine seeds. Raining was applied with a semi-solid device with sprinklers during periods of greatest water demand. It was shown that watered plants produced seeds of lesser quality, having smaller size and weight. To find out why seeds of irrigated plants were of poor quality, interdisciplinary research at the cellular level was carried out. DNA cytophotometry evidenced the presence of nuclei with lower polyploidy in the apical zone of mature seeds. This may lead to formation of smaller cells and reduce depositing of storage materials. The electrophoretic and Fourier transform infrared spectroscopic analyses revealed differences in protein and cuticular wax profiles, while scanning electron microscopy and energy dispersive spectroscopy revealed, among various chemical elements, decreased calcium content in one of seed zones (near plumule). Seeds from irrigated plants showed slightly higher germination dynamics but growth rate of seedlings was slightly lower. The studies showed that irrigation of lupine affected seed features and their chemical composition, an ability to germination and seedlings growth.


Assuntos
Irrigação Agrícola , Lupinus/química , Sementes/química , 3,3'-Diaminobenzidina/metabolismo , Cotilédone/metabolismo , DNA de Plantas/genética , Germinação , Peróxido de Hidrogênio/análise , Meristema/metabolismo , Mitose , Proteínas de Plantas/metabolismo , Plântula/crescimento & desenvolvimento , Sementes/anatomia & histologia , Sementes/ultraestrutura , Espectrometria por Raios X , Espectroscopia de Infravermelho com Transformada de Fourier
16.
Int J Mol Sci ; 20(20)2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31623090

RESUMO

The floral development in an important legume crop yellow lupine (Lupinus luteus L., Taper cv.) is often affected by the abscission of flowers leading to significant economic losses. Small non-coding RNAs (sncRNAs), which have a proven effect on almost all developmental processes in other plants, might be of key players in a complex net of molecular interactions regulating flower development and abscission. This study represents the first comprehensive sncRNA identification and analysis of small RNA, transcriptome and degradome sequencing data in lupine flowers to elucidate their role in the regulation of lupine generative development. As shedding in lupine primarily concerns flowers formed at the upper part of the inflorescence, we analyzed samples from extreme parts of raceme separately and conducted an additional analysis of pedicels from abscising and non-abscising flowers where abscission zone forms. A total of 394 known and 28 novel miRNAs and 316 phased siRNAs were identified. In flowers at different stages of development 59 miRNAs displayed differential expression (DE) and 46 DE miRNAs were found while comparing the upper and lower flowers. Identified tasiR-ARFs were DE in developing flowers and were strongly expressed in flower pedicels. The DEmiR-targeted genes were preferentially enriched in the functional categories related to carbohydrate metabolism and plant hormone transduction pathways. This study not only contributes to the current understanding of how lupine flowers develop or undergo abscission but also holds potential for research aimed at crop improvement.


Assuntos
Flores/genética , Regulação da Expressão Gênica de Plantas , Lupinus/genética , Desenvolvimento Vegetal/genética , RNA de Plantas/genética , Pequeno RNA não Traduzido/genética , Transcriptoma , Biologia Computacional/métodos , Evolução Molecular , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Redes e Vias Metabólicas , Família Multigênica , Fenótipo , Interferência de RNA , Estabilidade de RNA , Reprodutibilidade dos Testes
17.
Plant Foods Hum Nutr ; 74(4): 508-517, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31630281

RESUMO

In recent years, interest in plant-based proteins has been rising due to ethical and sustainability issues. In this context, the production of protein concentrates and isolates from new plant sources have increased enormously because of their nutritional and techno-functional properties. Therefore, this work describes a pilot process for obtaining protein-rich ingredients from a yellow lupin variety (Lupinus luteus) developed by the Agriaquaculture Nutritional Genomic Center (CGNA). A protein alkaline solubilisation followed by isoelectric precipitation was used as a method for obtaining a protein concentrate (LPC) and isolate (LPI) with 75 and 95% protein, respectively. The changes in the protein quality and chemical composition during the protein concentration process from lupin flour were evaluated. Thus, nutritional parameters such as the amino acids (AAs) profile, essential amino acid index (EAAI), chemical score (CS), the protein efficiency ratio (PER), and digestible indispensable amino acid score (DIAAS) were determined to evaluate the nutritional quality of LPC and LPI. The facile and scalable protein isolation method without a defatting process proposed in this study showed a great protein separation efficiency (PSE), while the amino acids profile was not affected during both the concentration as well as the isolation of proteins. In addition, SDS-PAGE showed that both LPC and LPI mainly contained the high-molecular-weight proteins α and ß-conglutins. Both LPC and LPI had a balanced amino acids profile, and arginine was the most predominant amino acid. These results are useful for increasing the use of lupin based-protein ingredients as a potential functional ingredient in the food industry.


Assuntos
Lupinus , Aminoácidos , Valor Nutritivo , Proteínas de Plantas , Sementes
18.
J Oleo Sci ; 68(11): 1063-1071, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31611511

RESUMO

In this study, some physico-chemical properties, amino acids, fatty acids, sugars and mineral contents of sorghum, millet and lupin seeds. Sorghum (red, white and yellow) and millet seeds were purchased from market in Saudi Arabia (Riyadh). Lupin seeds were provided from in Turkey (Konya). Protein contents of seed samples ranged from 8.6% (yellow sorghum) to 37.7% (lutop) (p < 0.05). The extractable phenolics contents for gallic acid equivalent (GAE) of grains ranged between 1.43 mgGAE/g (white sorghum) to 8.23 mgGAE/g (red sorghum), and hydrolysable phenolics contents for GAE of grains varied between 1.48 mgGAE/g (white sorghum) to 26.10 mgGAE/g (red sorghum (p < 0.05). Total phenol contents of seeds were found between 2769 mg GAE/g (bablon) to 6087 mgGAE/g (yellow sorghum) (p < 0.05). Amino acid contents of millet changed between 0.02% (ornithine) and 2.07% (glutamic acid), while amino acid contents of yellow sorghum range from 0.02% (hydroxyproline) to 1.71% (glutamic acid), amino acid values of white sorghum changed between 0.02% (hydroxyproline) and 2.21% (glutamic acid), amino acid values of lutop seed changed between 0.02% (ornithine) and 6.77% (glutamic acid) (p < 0.05).While the oleic acid contents change between 25.27% (white sorghum) and 53.50% (Bablone), linoleic acid contents ranged from 14.60% (Bablone) to 42.67% (Millet) (p < 0.05). However, the amount of potassium in the seeds varied between 1831.34 mg/kg (while sorghum) and 11895.8 mg/kg (Lutop). Generally, protein, oleic acid, amino acid and mineral contents of lupin varieties were higher as compared to those of millet phenol, anthocyanin and sorghum seeds.


Assuntos
Aminoácidos/análise , Carboidratos/análise , Ácidos Graxos/análise , Lupinus/química , Milhetes/química , Minerais/análise , Sementes/química , Sorghum/química , Fenômenos Químicos , Ácido Gálico/análise , Hidroxibenzoatos/análise , Proteínas de Plantas/análise , Arábia Saudita , Turquia
19.
Syst Appl Microbiol ; 42(6): 126015, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31591000

RESUMO

Three bacterial strains, LmiM8T, LmiE10 and LluTb3, isolated from nitrogen-fixing nodules of Lupinus micranthus (Lmi strains) and L. luteus (Llu strain) growing in Northern Tunisia were analysed using genetic, phenotypic and symbiotic approaches. Phylogenetic analyses based on rrs and concatenated gyrB and dnaK genes suggested that these Lupinus strains constitute a new Microvirga species with identities ranging from 95 to 83% to its closest relatives Microvirga makkahensis, M. vignae, M. zambiensis, M. ossetica, and M. lotononidis. The genome sequences of strains LmiM8T and LmiE10 exhibited pairwise Average Nucleotide Identities (ANIb) above 99.5%, significantly distant (73-89% pairwise ANIb) from other Microvirga species sequenced (M. zambiensis and M. ossetica). A phylogenetic analysis based on the symbiosis-related gene nodA placed the sequences of the new species in a divergent clade close to Mesorhizobium, Microvirga and Bradyrhizobium strains, suggesting that the M. tunisiensis strains represent a new symbiovar different from the Bradyrhizobium symbiovars defined to date. In contrast, the phylogeny derived from another symbiosis-related gene, nifH, reproduced the housekeeping genes phylogenies. The study of morphological, phenotypical and physiological features, including cellular fatty acid composition of the novel isolates demonstrated their unique profile regarding close reference Microvirga strains. Strains LmiM8T, LmiE10 and LluTb3 were able to nodulate several Lupinus spp. Based on genetic, genomic and phenotypic data presented in this study, these strains should be grouped within a new species for which the name Microvirga tunisiensis sp. nov. is proposed (type strain LmiM8T=CECT 9163T, LMG 29689T).


Assuntos
Lupinus/microbiologia , Methylobacteriaceae/classificação , Filogenia , Nódulos Radiculares de Plantas/microbiologia , Antibacterianos/farmacologia , Ácidos Graxos/química , Genes Bacterianos/genética , Genes Essenciais/genética , Methylobacteriaceae/química , Methylobacteriaceae/efeitos dos fármacos , Methylobacteriaceae/genética , Fenótipo , Análise de Sequência de DNA , Especificidade da Espécie , Simbiose/genética , Tunísia
20.
Nutrients ; 11(9)2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31492011

RESUMO

Interference of dietary fibres with the enterohepatic circulation of bile acids is proposed as a mechanism for lowering cholesterol. We investigated how lupin hull and cotyledon dietary fibres interact with primary bile acids using an in vitro model under simulated upper gastrointestinal conditions. Cell wall polysaccharides were isolated and extracted to separate pectin-like, hemicellulosic, and lignocellulosic structures. Lupin hull consisted mainly of structural components rich in cellulose. The viscosity of the in vitro digesta of lupin hull was low, showing predominantly liquid-like viscoelastic properties. On the other hand, lupin cotyledon fibre retarded bile acid release due to increased viscosity of the in vitro digesta, which was linked with high contents of pectic polymers forming an entangled network. Molecular interactions with bile acids were not measured for the hull but for the cotyledon, as follows: A total of 1.29 µmol/100 mg DM of chenodesoxycholic acids were adsorbed. Molecular interactions of cholic and chenodesoxycholic acids were evident for lignin reference material but did not account for the adsorption of the lupin cotyledon. Furthermore, none of the isolated and fractionated cell wall materials showed a significant adsorptive capacity, thus disproving a major role of lupin cell wall polysaccharides in bile acid adsorption.


Assuntos
Ácidos e Sais Biliares/metabolismo , Parede Celular/metabolismo , Fibras na Dieta/metabolismo , Digestão , Lupinus/metabolismo , Polissacarídeos/metabolismo , Adsorção , Cotilédone/metabolismo , Elasticidade , Interações Hidrofóbicas e Hidrofílicas , Viscosidade
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