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1.
Environ Sci Pollut Res Int ; 27(9): 9038-9057, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31893365

RESUMO

Multiple drug resistance and increased side effects due to allopathic drugs has warned scientific community with a global alarm to identify molecules from natural sources to combat diseases with minimum or no side effects. The present investigation was aimed to identify and isolate secondary metabolites from traditionally used Nerium indicum using conventional column chromatography which led to the isolation of two compounds, C-I (fractions NB4f1) and C-II (fractions NC13b1). Further characterized, it is elucidated using spectral data and identified as N-(4-hydroxy-phenyl)-2-methoxy-2-phenyl-acetamide, molecular formula C15H15NO3, and molecular weight 257.3 (C-I) and N-(4-hydroxy-phenyl)-2-phenyl-N-phenylacetyl-acetamide, molecular formula C22H19NO3, and molecular weight 345.4 (C-II). Further, the isolated compounds were investigated using in silico approach by Autodock tool with four different proteins specific for cancer and in vitro assessed cell proliferation, and apoptosis against human breast cancer MCF 7 cell line. The results of the in silico model demonstrated potent binding affinity of both compounds with the proteins representing that the isolated molecules could be a drug of choice for cancer. Further, the isolated compounds revealed significant inhibition of cell proliferation (IC50 values 21 µg/mL for C-I, 19 µg/mL for C-II) with induced apoptosis with nuclear condensation effect on the MCF 7 cells in in vitro condition even at very low concentration. Compound treatment to MCF-7 cell line represented bright fetches indicating condensed chromatins and higher level of nuclear fragmentation with DAPI staining, indicating higher cell death due to induced apoptosis and confirmed using flow cytometry analysis representing inhibition of cell proliferation at S phase. Graphical abstract.


Assuntos
Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Nerium , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Humanos , Células MCF-7 , Fenóis/farmacologia
2.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2283-2291, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359655

RESUMO

To build up an identification method on cardiac glycosides in Taxillus chinensis and its Nerium indicum host, and evaluate the influence on medicine quality from host to T. chinensis, ultra-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass-mass spectrometry(UPLC-Q-TOF-MS/MS)was applied. The samples of T. chinensis(harvested from N. indicum)and its N. indicum host were collected in field. The samples of T. chinensis(harvested from Morus alba)and its M. alba host was taken as control substance. All samples were extracted by ultrasonic extraction in 70% ethanol. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 C_(18)(2.1 mm×100 mm,1.8 µm)column at 40 ℃. Gradient elution was applied, and the mobile phase was consisted of 0.1% formic acid water and acetonitrile. The 0.5 µL of sample solution was injected and the flow rate of the mobile phase was kept at 0.6 mL·min~(-1) in each run. It was done to identify cardiac glycosides and explore the chemical composition correlation in T. chinensis and its N. indicum host by analyzing positive and negative ion mode mass spectrometry data, elemental composition, cardiac glycoside reference substance and searching related literatures. A total of 29 cardiac glycosides were identified, 28 of it belonged to N. indicum host, 5 belonged to T. chinensis(harvested from N. indicum host), none of cardiac glycoside was identified in T. chinensis(harvested from M. alba host). The result could provide a reference in evaluating the influence in T. chinensis medicine quality from host. It was rapid, accurate, and comprehensive to identify cardiac glycosides in T. chinensis and its N. indicum host by UPLC-Q-TOF-MS/MS.


Assuntos
Glicosídeos Cardíacos/análise , Medicamentos de Ervas Chinesas/química , Loranthaceae/química , Nerium/química , Cromatografia Líquida de Alta Pressão , Compostos Fitoquímicos/análise , Espectrometria de Massas em Tandem
3.
Appl Radiat Isot ; 152: 18-24, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31279149

RESUMO

In this study, Nerium oleander L. leaves were used as biomonitors to evaluate environmental pollutants levels in a sub-region in the Metropolitan Area of Rio de Janeiro City (Brazil) through X-ray Fluorescence (XRF). Control samples were collected in a rural zone next to the Parque Estadual da Pedra Branca/RJ. The samples were collected during all seasons of 2015 (summer, fall, winter and spring). The concentration of 13 elements (S, Cl, K, Ca, Mn, Fe, Cu, Zn, Br, Rb, Sr, Ba and Pb) was determined. Most of the elements showed, during all seasons, median concentrations of sampling sites higher than the results obtained from the control sites. The principal component analysis (PCA) showed the grouping of the elements in two main factors that can be associated to emission sources of these elements. The elements Cl, K, Ca, Cu, Rb and Sr can be associated as a possible influence from the soil (root uptake and/or resuspension). On the other hand, the elements Fe, Cu, Zn, and Pb can be associated to vehicle and industry emission sources. The study showed that the XRF technique applied to Nerium oleander L. leaves is efficient to environmental pollution analysis in Metropolitan Regions since it is precise, fast and low-cost, besides allowing the monitoring of pollution levels over time.


Assuntos
Poluição do Ar/análise , Metais Pesados/análise , Nerium/química , Folhas de Planta/química , Brasil , Cidades , Monitoramento Ambiental/métodos , Espectrometria por Raios X
4.
Eur J Med Chem ; 180: 417-429, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31325787

RESUMO

Oleandrin, the major biologically active constituent of shrub Nerium oleander preparations of which have been used in traditional Mediterranean and Asian medicine, attracts a great deal of attention due to its pronounced anticancer activity. The synthesis of oleandrigenin model, 16ß-hydroxy-3ß-methoxy-5α-card-20(22)-enolide 16-acetate, from androstenolone acetate through 17ß-(3-furyl)-intermediates has been developed. Several related 17ß-(butenolidyl)- and 17ß-(furyl)-androstane derivatives were synthesized and tested for in vitro cytotoxic and Na+/K+-ATP-ase inhibitory activities. Comparison of Na+/K+-ATP-ase inhibitory and cytotoxic activity underlines complex nature of the relationship.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Cardenolídeos/farmacologia , Inibidores Enzimáticos/farmacologia , ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores , Antineoplásicos Fitogênicos/síntese química , Antineoplásicos Fitogênicos/química , Cardenolídeos/síntese química , Cardenolídeos/química , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Humanos , Conformação Molecular , Nerium/química , ATPase Trocadora de Sódio-Potássio/metabolismo , Relação Estrutura-Atividade
5.
J Forensic Leg Med ; 65: 133-136, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31153008

RESUMO

BACKGROUND: Benefits and even dangers of plants are known since time began. The ancients used plants and herbs because of their effects on the human body. Poisoning is a logical consequence of their use: history is full of episodes of plants and herbs poisoning, whether intentional or accidental. AIM: Oleander poisoning is generally accidental; an intentional assumption of its leaves to commit suicide is uncommon because the population is not aware of the harmfulness of its cardiotoxic glycosides, therefore we report a fatal case of self-poisoning through the voluntary ingestion of oleander leaves. METHODS: A diagnosis of oleander self-poisoning was highly suspected on the basis of the circumstantial evidence and the autopsy findings. Toxicological investigations were performed on the samples collected during the autopsy and aimed at confirm the presence of oleandrin at a toxic level. RESULTS: The autopsy revealed a piece of oleander leaf on the posterior third of the tongue's body and several plant residues, similar to the one recovered on the tongue, into the gastric content; petechiae on the deep surface of the scalp, multi-organ congestion, and pulmonary edema were also observed. The histological study corroborated the pulmonary edema macroscopically observed but did not provide any other information. The detection of oleandrin in biological cadaveric samples revealed high, fatal, concentrations. CONCLUSIONS: Cases of voluntary ingestion of oleander with a suicidal intent prove to be uncommon: in the case reported the victim was aware about the possibility to commit suicide through the ingestion of oleander leaves.


Assuntos
Nerium/envenenamento , Folhas de Planta/envenenamento , Suicídio , Química Encefálica , Cardenolídeos/análise , Feminino , Vesícula Biliar/química , Mucosa Gástrica/química , Conteúdo Gastrointestinal/química , Humanos , Rim/química , Fígado/química , Pulmão/química , Pessoa de Meia-Idade , Edema Pulmonar/patologia , Baço/química
6.
Onderstepoort J Vet Res ; 86(1): e1-e9, 2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31170782

RESUMO

Shrubs represent the most affordable and accessible form of feed that livestock can rely on to acquire both essential and non-essential elements of life. In addition to their inherent toxins, they contain endogenous substances commonly referred to as 'antinutritive factors' (ANFs) that often interfere with the utilisation of nutrients. Their abundance may lead to severe clinical trauma. Hence, the objective of the study was to investigate the effects of different extraction techniques on Nerium oleander L. and animal feeds as well as to quantify oxalates. Organic (hexane, acetone and methanol) sequential and aqueous (infusion and decoction) extractions were explored. Qualitative and quantitative analyses were conducted to determine the presence of various phytochemicals and oxalate contents as putative ANFs, respectively. The results showed higher extraction yields of 22.6% and 43.1% in the decoction and infusion of N. oleander, respectively. The quantification methods were validated for linearity, accuracy and precision. Oxalate contents of 6.76 ± 0.245 (0.65%) mg/g and 5.74 ± 0.236 mg/g dry weight (0.55%) were obtained in N. oleander and feeds, respectively. This difference was statistically significant with p < 0.05. Percentage recoveries of 98.5 (percent relative standard deviation [% RSD] = 2.3), 85.7 (% RSD = 1.03) and 80.3 (% RSD = 1.22) at 76%, 95% and 112% fortifications were obtained, respectively. Relative standard deviation for precision was 0.99% and 1.13% at 0.33 mg and 0.39 mg fortifications, respectively, while reproducibility showed 2.21% RSD. Therefore, these methods can be used to provide a valuable basis for qualitative determination of ANFs, particularly in shrub foliage.


Assuntos
Ração Animal/análise , Criação de Animais Domésticos , Nerium/química , Oxalatos/análise , Extratos Vegetais/análise , Animais , Cromatografia Líquida de Alta Pressão/veterinária , Gado , Folhas de Planta/química , Reprodutibilidade dos Testes , África do Sul
7.
J Pharm Biomed Anal ; 174: 450-459, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31220703

RESUMO

To evaluate the effect of the host plant on the quality of Loranthaceae species as medicinal raw material, ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used to identify cardiac glycosides in Nerium indicum and its parasitic plant species Taxillus chinensis and Scurrula parasitica. Samples were collected from N. indicum and these parasites, while Morus alba and its parasite T. chinensis and Osmanthus fragrans and its parasite S. parasitica were used as controls. Based on mass spectrometry data and elemental composition analysis of positive and negative ion modes, in combination with standard cardiac glycosides and relevant literature, cardiac glycosides in N. indicum and its parasites T. chinensis and S. parasitica were identified, and their correlations were analyzed. A total of 29 cardiac glycosides were identified, among which 28 were found in N. indicum parasitized by T. chinensis; 25 cardiac glycosides were identified in the same host under attack by S. parasitica; five cardiac glycosides were identified in both T. chinensis and S. parasitica, which grew parasitically on N. indicum, whereas no cardiac glycosides were identified in M. alba parasitized by T. chinensis, or in O. fragrans parasitized by S. parasitica. We conclude that UPLC-Q-TOF-MS/MS technology can identify cardiac glycosides in N. indicum and parasites T. chinensis and S. parasitica rapidly, accurately, and thoroughly. N. indicum will transfer its own cardiac glycosides to its parasites through the special host-parasite interaction. Our results provide a reference basis for evaluating the influence of the host plant on the quality of medicinal compounds obtained from Loranthaceae species.


Assuntos
Glicosídeos Cardíacos/química , Loranthaceae/química , Nerium/química , Cromatografia Líquida de Alta Pressão , Digitoxigenina/análise , Doxorrubicina/análogos & derivados , Doxorrubicina/análise , Medicamentos de Ervas Chinesas/química , Flavonoides/química , Estrutura Molecular , Plantas Medicinais/química , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
8.
Environ Sci Pollut Res Int ; 26(16): 16097-16104, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30968298

RESUMO

The phyllosphere provides appropriate conditions for colonization by microorganisms, including diazotrophic bacteria. However, a poor understanding of the effects of the atmospheric environment on the phyllospheric diazotrophic communities persists. We detected the biodiversity, abundance, and activity of nitrogen-fixing bacteria in the phyllospheres of two evergreen shrubs, Nerium indicum Mill. and Osmanthus sp., sampled from urban areas with heavy traffic, a college campus, and a forest. Quantitative PCR analysis indicated that the copy numbers of nifH sequences were highest in the phyllospheres of both plants in heavy-traffic urban areas and correlated with the recorded nitrogenase activities of the phyllospheres. Similarly, the phyllosphere from heavy-traffic urban areas also possessed the highest biodiversity indices of diazotrophic communities from both the two plants. Pyrosequencing analysis revealed a diversity of nifH sequences in phyllosphere that were mostly uniquely found in the phyllosphere, and many of these were proteobacteria-like and cyanobacteria-like. Members of the Proteobacteria, mostly of which were not closely related to unknown organisms, were detected exclusively in the phyllosphere and represented substantial fractions of their associated diazotrophic communities. Our study provides initial insight into the shifts in the biodiversity and community structure of N2-fixing microorganisms in the phyllospheres of different atmospheric environments.


Assuntos
Nerium/microbiologia , Bactérias Fixadoras de Nitrogênio/fisiologia , Oleaceae/microbiologia , Biodiversidade , Cianobactérias/genética , Ecossistema , Florestas , Oxirredutases/genética , Proteobactérias/genética , Reação em Cadeia da Polimerase em Tempo Real , Urbanização
9.
Phytomedicine ; 57: 352-363, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30831484

RESUMO

BACKGROUND: Nerigoside (NG), a cardenolide isolated from a commonfolk medicine, Nerium oleander Linn. (Apocynaceae), has not been explored for its biological effects. To date, cardenolides have received considerable attention in pharmacology studies due to their direct effects of apoptosis-induction or growth-inhibitory against tumor in vitro and in vivo. Whether and how NG exerts anticancer effects against colorectal cancer remains to be elucidated. PURPOSE: The aim of this study was to investigate the anticancer effect of NG in human colorectal cancer cells. METHODS: To test anticancer effect, we compared potency of NG in two colorectal cancer cell lines, HT29 and SW620 by WST-1 and colony proliferation assays. And we investigated mechanism of anticancer activities by analyzing players in apoptotic and ERK/GSK3ß/ß-catenin signaling pathways in HT29 and SW620 cells treated with NG. RESULTS: In this study, we showed that NG markedly suppressed the cell viability and colony formation of colorectal cancer cells HT29 and SW620, with no significant toxic effect on non-cancer cells NCM460. Annexin V-FITC/PI and CFSE labeling results revealed that NG suppressed cell proliferation in low concentration, along with reducing expression of PCNA, while NG induced apoptosis in high concentration,. Meanwhile, NG significantly arrested cell migration by reversal of EMT and cell cycle on G2/M. Then, we found that the ERK and GSK3ß/ß-catenin signaling pathway were noticeably blocked in CRC cells after treatment with NG. According to western blot, NG upregulated the expression of p-GSK3ß/GSK3ß and decreased especially the expression of ß-catenin in nuclear. In addition, Wnt signaling and its target genes were suppressed in response to NG. Then, the Ser9 phosphorylation of GSK3ß can be reduced / raised by GÖ 6983 / LiCl, respectively. Thus, we further confirmed that the GSK3ß/ß-catenin axis is involved in NG-prevented cell proliferation. CONCLUSION: NG inhibited the growth of colorectal cancer cells by suppressing ERK/GSK3ß/ß-catenin signaling pathway. And the GSK3ß/ß-catenin axis is involved in preventing cell proliferation and migration by NG-treatment. These results suggest that NG may be used to treat colorectal cancer, with better outcome by combining with GSK3ß inhibitor to block Wnt pathway.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/patologia , Glicogênio Sintase Quinase 3 beta/metabolismo , beta Catenina/metabolismo , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Neoplasias Colorretais/metabolismo , Glicogênio Sintase Quinase 3 beta/antagonistas & inibidores , Células HT29 , Humanos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Terapia de Alvo Molecular/métodos , Nerium/química , Fosforilação/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , beta Catenina/antagonistas & inibidores
10.
Int J Syst Evol Microbiol ; 69(1): 285-290, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30511915

RESUMO

A Gram-stain-positive, aerobic, short-rod-shaped, non-spore-forming actinobacterial strain, designated M8JJ-5T, was isolated from a surface-sterilized bark of Neriumindicum Mill. collected from Guizhou, China, and investigated by a polyphasic approach to determine its taxonomic position. Strain M8JJ-5T grew optimally without NaCl at 28 °C and at pH 7.0-8.0. Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain M8JJ-5T was most closely related to the type strains of genus Amnibacterium, and shared highest 16S rRNA gene sequence similarity of 97.29 % to Amnibacterium kyonggiense KSL51201-037T. The DNA G+C content of strain M8JJ-5T was 68.6 mol%. The cell-wall peptidoglycan contained l-2,4-diaminobutyric acid and MK-12, MK-11 were the major menaquinones. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid, while the major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain M8JJ-5T can be characterized to represent a novel species of the genus Amnibacterium, for which the name Amnibacteriumflavum sp. nov. is proposed. The type strain is M8JJ-5T (=KCTC 49089T=CGMCC 1.16390T).


Assuntos
Actinomycetales/classificação , Nerium/microbiologia , Filogenia , Casca de Planta/microbiologia , Actinomycetales/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Parede Celular/química , China , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Peptidoglicano/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/química
11.
Int J Biol Macromol ; 121: 655-665, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30336237

RESUMO

Cellulose-based substrates could represent potential funds for the sorption of pollutants. Herein, methylene blue was selected for demonstrating the bio-sorption efficiency of Nerium oleander, Pergularia tomentosa and Populus tremula seed fibers. Their cellulose contents were 45%, 43.8% and 60%. Their lignin amounts were 21%, 8.6% and 12%, respectively. Fourier Transform InfraRed suggested that the interaction of these bio-products with methylene blue could occur between hydroxyl and ester groups of cellulose and lignin and the sulfur and nitrogen atoms of the dye. Scanning Electron Microscopy showed a swelling of the bio-matters after dye biosorption. From X-Ray Diffraction, the shifting for higher values of the peaks related to the amorphous phase indicated the establishment of new rearranged regions. Such change from the decomposition behavior event studied by Thermogravimetric Analysis/Differential Thermal Analysis revealed that methylene blue was interacted with cellulose and lignin structures. The effect of adsorbent dosage, pH, time, dye concentration and temperature was investigated in controlled batch experiments. Excellent sorption capacities followed the order: Nerium oleander (280.2 mg g-1) > Populus tremula (168 mg g-1) > Pergularia tomentosa (145.3 mg g-1). Freundlich fitted best the equilibrium data suggesting cooperative interactions via physisorption and chemisorption phenomenon. Kinetic data complied well with the pseudo-second-order suggesting a chemisorption mechanism.


Assuntos
Celulose/química , Lignina/química , Azul de Metileno/química , Azul de Metileno/isolamento & purificação , Nerium/química , Populus/química , Sementes/química , Adsorção , Cinética , Temperatura
12.
J Photochem Photobiol B ; 189: 274-282, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30428450

RESUMO

Spectral Solar Photosynthetically Photon Flux Density (PPFD) (380 to 780 nm) reaching the surface of a plant in different lighting conditions has been analyzed in order to better understand the different photosynthetic performance of plants depending on their spatial situation and the vegetation surrounding. A comparison between the shadow of several trees in a sunny day and the case of a cloudy day in an open space has been studied. Three isolated trees (a palm tree, an olive tree and a shrub oleander) and a tipuana grove have been studied. The study has been developed in Valencia (Spain) during January and February 2017. A portable Asensetek Standard ALP-01 spectrometer with a measurement wavelength range of 380 to 780 nm, has been used. Conditions with higher PPFD received are found to be, apart from those of a sunny day, those for cloudy day (with a spectral maximum in the Green region of the spectrum), and those for individual trees and shrub shadows in a sunny day (with a spectral maximum in the Blue region). The case in which less amount of PPFD is received is that under the shadow of tipuana grove (with a spectral maximum in the Infrared region of the spectrum). In fact the order of magnitude in which the PPFD in a cloudy day exceeds the PPFD under the tipuana grove shade is up to 20.


Assuntos
Fotossíntese , Folhas de Planta/efeitos da radiação , Luz Solar , Tempo (Meteorologia) , Nerium , Olea , Phoeniceae , Espanha , Árvores
13.
Ann Agric Environ Med ; 25(3): 449-452, 2018 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-30260192

RESUMO

INTRODUCTION: Weeds are an important factor in determining production in the agricultural sector. Currently, weeds have been managed by employing synthetic weedicides chemicals. These chemicals have a negative impact on the environment through accumulation in soil and water, in addition to their effect on biological diversity. MATERIAL AND METHODS: A study was undertaken to evaluate the activity of the aqueous extract of leaves from; Nerium (Nerium oleander L), olives (Olea europaea L) and castor (Ricinus communis L) on the control of Cyperus rotundus L. The study comprised of two components: three plant species, and the uses of three planta extracts concentration (5%, 7.5% and 10%) and their impact on germination and inhibition of seedling growth. RESULTS: The results from using Nerium extract in comparison with Tibenuron and the two other plants extracts gave 90% inhibition at concentrations of 5% and 10%, while the concentration of 2.5% gave the lowest inhibition rates for germination of all plant extracts under study. The second experiment carried out using 10% concentration of the Nerium extract gave complete inhibition (100%), compared with the same concentrations of other plants extract and weedicides. CONCLUSIONS: The current study indicates the possibility of using natural products of plant origin as alternatives to chemical herbicides as one of the means of biological control to reducing environment pollution.


Assuntos
Poluição Ambiental/prevenção & controle , Herbicidas/farmacologia , Nerium/química , Olea/química , Feromônios/farmacologia , Extratos Vegetais/farmacologia , Plantas Daninhas/efeitos dos fármacos , Ricinus/química , Alelopatia , Germinação/efeitos dos fármacos , Herbicidas/análise , Feromônios/análise , Extratos Vegetais/análise , Folhas de Planta/química , Plantas Daninhas/crescimento & desenvolvimento , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Controle de Plantas Daninhas
14.
Can J Gastroenterol Hepatol ; 2018: 4739637, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29850455

RESUMO

This study was aimed to find histological changes in the extrahepatic organs, hepatic iron deposition, and gene expression of some iron regulatory proteins in rats after sterile muscle abscess during the acute intoxication of Nerium oleander leaves decoction. 10 ml/kg of the leaves extract was injected intramuscularly in Wistar rats (200-225 g, n = 4). Control animals received saline injection of matched volume. Animals were anesthetized and sacrificed after 3, 6, 12, and 24 h after administration of decoction. Lungs, kidney, spleen, and liver were extracted and processed for histopathological examination while portion of liver tissue was proceeded for iron regulatory gene expression quantification. Sections of all studied organs were found with signs of cellular dysfunction with infiltration of variety of leucocytes. In the lungs section at 3 h time point mononuclear cell infiltrates were observed while in alveolar tissue at 24 h time point dilation and even collapse in some of the alveoli were evident. In kidney sections distortion of renal tubules and epithelial cells with shrinkage of glomeruli was noted at all studied time points. In the splenic section of 12 h time point, degeneration, depopulation, and shrinkage of white pulp have been noted. Distension of the red pulp along with activation of splenic follicles was evident after 24 h onset of APR. Significant changes in the expression of acute phase cytokine and iron regulatory genes were noted. IL-6 and Hepc gene expression were strongly upregulated up to 12 h whereby Tf gene expression showed an early upregulation at 3 h time point followed by downregulation on later points while Hjv gene expression showed an overall downregulation at all study time points compared to control. It is concluded that inherent toxins present in the N. oleander can induce acute phase response and cause severe histological changes in the organs and marked changes in the regulation of iron regulatory proteins thus cannot be practiced routinely.


Assuntos
Insuficiência de Múltiplos Órgãos/induzido quimicamente , Insuficiência de Múltiplos Órgãos/patologia , Nerium/toxicidade , Folhas de Planta , Animais , Biópsia por Agulha , Modelos Animais de Doenças , Imuno-Histoquímica , Rim/efeitos dos fármacos , Rim/patologia , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/patologia , Paquistão , RNA/análise , Distribuição Aleatória , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real/métodos , Valores de Referência , Baço/efeitos dos fármacos , Baço/patologia
15.
Ying Yong Sheng Tai Xue Bao ; 29(5): 1653-1659, 2018 May.
Artigo em Chinês | MEDLINE | ID: mdl-29797899

RESUMO

The diversity of phyllosphere bacteria is one of the hotspots in the research area of plant-microbial relationship. There are still a lot of controversies in the main factors influencing community structure of phyllosphere bacteria. In this study, the phyllosphere bacterial community structure of Osmanthus fragrans and Nerium indicum grown in three habitats was investigated based on high-throughput sequencing and the main driving factors were examined. The results showed that there was no significant difference in phyllosphere bacterial diversity between the two plant species grown in three habitats. For phyllosphere bacterial community of two plant species from three habitats, the dominant phyla were Actinobacteria, Bacteroidetes, Chlamydiae, Cyanobacteria, Firmicutes and Proteobacteria, and the dominant genera included Methylobacterium, Sphingomonas, Hymenobacter, Polaromonas and Spirosoma. The structure of phyllosphere bacterial community was influenced by habitats, host species identity and species-habitat interaction, among which habitat showed the strongest effect.


Assuntos
Ecossistema , Nerium/microbiologia , Microbiologia do Solo , Bactérias , Sequenciamento de Nucleotídeos em Larga Escala , Filogenia , Plantas , RNA Ribossômico 16S
17.
J Nat Prod ; 81(5): 1235-1240, 2018 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-29693393

RESUMO

Notch signaling plays a crucial role in differentiation and cell maintenance, but once aberrantly activated, it contributes to cancer progression. Notch inhibitors were isolated from plant extracts and tested using an originally constructed cell-based assay system. We isolated eight compounds from Nerium indicum that showed inhibition of the Notch signaling pathway. HES1 and HES5 are target genes of the Notch signaling pathway, and oleandrin (1) decreased the protein levels of HES1 and HES5 in assay cells. Oleandrin (1) showed potent cytotoxicity against HPB-ALL cells and decreased HES1 and the Notch intracellular domain in these cells. The main mechanism of action of 1 appears to be inhibition of Notch signaling by acceleration of Notch intracellular domain degradation.


Assuntos
Nerium/química , Receptores Notch/antagonistas & inibidores , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Cardenolídeos/química , Cardenolídeos/farmacologia , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Linhagem Celular Tumoral , Citotoxinas/química , Citotoxinas/farmacologia , Células HEK293 , Humanos , Transdução de Sinais/efeitos dos fármacos , Fatores de Transcrição HES-1/metabolismo
18.
Fitoterapia ; 127: 293-300, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29540313

RESUMO

Six new cardenolides (1-6), including three 14-hydroxylated cardenolides and three 14-carbonylated cardenolides were isolated from the dried aerial parts of Nerium oleander Linn in addition to twenty-seven known compounds (7-33). Their structures were elucidated on the basis of extensive spectroscopic evidences and single-crystal X-ray diffraction analysis. Compounds 1, 4, 7-10 and 13 exhibited significant cytotoxicity against four colon cancer cell lines (HCT116, HT29, SW620, RKO), one gastric cancer cell line (GT) and one cervical cancer cell line (HeLa) in vitro.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Cardenolídeos/isolamento & purificação , Nerium/química , Folhas de Planta/química , Antineoplásicos Fitogênicos/farmacologia , Cardenolídeos/farmacologia , Linhagem Celular Tumoral , Humanos , Estrutura Molecular , Extratos Vegetais/química
20.
Environ Sci Technol ; 52(4): 2071-2080, 2018 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-29320848

RESUMO

A thorough understanding of spatial and temporal emission and immission patterns of air pollutants in urban areas is challenged by the low number of air-quality monitoring stations available. Plants are promising low-cost biomonitoring tools. However, source identification of the trace metals incorporated in plant tissues (i.e., natural vs anthropogenic) and the identification of the best plant to use remain fundamental challenges. To this end, Nerium oleander L. collected in the city of Zaragoza (NE Spain) has been investigated as a biomonitoring tool for assessing the spatial immission patterns of airborne metals (Pb, Cu, Cr, Ni, Ce, and Zn). N. oleander leaves were sampled at 118 locations across the city, including the city center, industrial hotspots, ring-roads, and outskirts. Metal concentrations were generally higher within a 4 km radius around the city center. Calculated enrichment factors relative to upper continental crust suggest an anthropogenic origin for Cr, Cu, Ni, Pb, and Zn. Zinc isotopes showed significant variability that likely reflects different pollution sources. Plants closer to industrial hotspots showed heavier isotopic compositions (δ66ZnLyon up to +0.70‰), indicating significant contributions of fly ash particles, while those far away were isotopically light (up to -0.95‰), indicating significant contributions from exhaust emissions and flue gas. We suggest that this information is applied for improving the environmental and human risk assessment related to the exposure to air pollution in urban areas.


Assuntos
Metais Pesados , Nerium , Cidades , Monitoramento Ambiental , Humanos , Folhas de Planta , Espanha , Isótopos de Zinco
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