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1.
Zhongguo Zhong Yao Za Zhi ; 45(2): 405-411, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-32237325

RESUMO

This work aimed to investigate the intestinal absorption characteristics of Laportea bulbifera extract in normal and rheumatoid arthritis model rats. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, kaempferol-3-O-rutinoside, galuteolin, quercetin and isoquercetin in intestinal absorption solution samples were detected by UPLC-MS/MS with 5.0 g·L~(-1) as the absorption concentration. The cumulative absorption(Q) and absorption rate constant(K_a) were calculated, and the absorption characteristics of different components of L. bulbifera in intestinal absorption solution of normal rats and rheumatoid arthritis rats were compared. The results showed that all the eight index components in the extract of L. bulbifera could be absorbed into the intestinal capsule, the cumulative absorption-time curve of each component showed an upward trend without saturation, and the correlation regression coefficient(R~2) was greater than 0.92, which is consistent with the zero-order absorption rate process. It was speculated that the possible absorption mode of each component was passive diffusion. In normal condition, the absorption of ileum was the best(except chlorogenic acid), and in pathological condition, duodenum was the best. The total absorption of 8 components in each intestinal segment of RA rats was better than that of normal rats, which speculated that rheumatoid arthritis may change the specific site of drug absorption. The experimental results showed that rheumatoid arthritis could change the intestinal absorption of the extract of L. bulbifera, and its mechanism needs further study.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Absorção Intestinal , Extratos Vegetais/uso terapêutico , Urticaceae/química , Animais , Cromatografia Líquida de Alta Pressão , Intestinos/efeitos dos fármacos , Ratos , Espectrometria de Massas em Tandem
2.
Biomed Chromatogr ; 34(2): e4734, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31692001

RESUMO

Laportea bulbifera, named Hong He Ma in Chinese, is a Chinese herbal medicine commonly used by the Miao nationality of China. In this study, 43 batches of L. bulbifera were collected from different origins in China. Ethanol, ethyl acetate and petroleum ether were used to prepare different extracts of the plant. UHPLC technique was used to establish the fingerprints, whereas DPPH assay and RAW264.7 inflammatory cell models were used to evaluate the antioxidant and anti-inflammatory activities, respectively. Moreover, the spectrum-effect relationship between relative peak area of common peaks and efficacy value was set up by multivariate statistical analysis. Furthermore, 10 batches were selected randomly for validation of those models. The results showed that ethyl acetate and petroleum ether extracts possess excellent antioxidant and anti-inflammatory activities, respectively. Peaks A6 and A7 demonstrated the greatest antioxidant activity, while peak A17 showed the strongest anti-inflammatory activity. After a verified experiment, the result was obtained and illustrated that the spectrum-effect relationship which we established could reliably infer antioxidant and anti-inflammatory compounds of the Chinese herbal medicine.


Assuntos
Anti-Inflamatórios , Antioxidantes , Extratos Vegetais , Urticaceae/química , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Camundongos , Análise Multivariada , Picratos/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Células RAW 264.7 , Fator de Necrose Tumoral alfa/antagonistas & inibidores
3.
J Ethnopharmacol ; 245: 112167, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31422110

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: We recently reported that ethanol extract of Myrianthus arboreus P. Beauv. root bark demonstrated antidiabetic activity by modulating hepatocyte glucose homeostasis. This activity was associated significantly to the ethyl acetate (EAc) fraction. The current study sought to identify the active compounds responsible of the antidiabetic effect of M. arboreus in the EAc fraction using bioassay-directed sub-fractionation. MATERIALS AND METHODS: EAc fraction was sub-fractionated using Flash chromatography. Preparative HPLC was used to isolate the pure compounds. The structures of the isolated compounds were confirmed by analysis of NMR spectroscopic and mass spectrometric data. Hepatic (H4IIE, HepG2) cells were treated with maximum non-toxic concentrations of ethanol extract, its EAc fraction and isolated compounds thereof. Glucose-6-phosphatase (G6Pase) activity was measured using the glucose oxidase method. To measure glycogen synthase (GS) activity, radioactive assays were used. Phosphorylation of AMP-activated protein kinase (AMPK) and Glycogen Synthase Kinase-3 (GSK-3) were probed by Western blot. RESULTS: Six sub-fractions were obtained, and the antidiabetic activity was found in two sub-fractions (SFE1 and SFE2). For the first time, two known C-glycosylflavone regio-isomers, isoorientin (1) and orientin (2) were detected and isolated from M. arboreus plant, especially from SFE2 as well as protocatechuic acid, 3,4-dihydroxybenzaldehyde (4), and chlorogenic acid isolated from SFE1. The compounds 1, 2, 4 were determined to decrease the activity of G6Pase by increasing AMPK phosphorylation and to stimulate GS through GSK-3 phosphorylation. Isoorientin which is one of the main compounds of EAc fraction, expressed the strongest effect in all bioassays, similar to that of the EAc fraction. A significant and linear correlation was found between the phosphorylation of AMPK and the activity of G6Pase modulated by all samples (R2 = 0.54; p < 0.05). Similar to G6Pase assay, a correlation was determined between the capacity of M. arboreus extracts/fractions/compounds to stimulate GS activity and to phosphorylate GSK-3 (R2 = 0.57, p < 0.01). CONCLUSION: Results demonstrate that 1, 2, 4 are responsible, at least in part, for the antidiabetic activity of M. arboreus. These compounds can be used to ensure the quality and efficacy of M. arboreus antidiabetic preparations and standardize such preparations.


Assuntos
Hipoglicemiantes/farmacologia , Fígado/efeitos dos fármacos , Compostos Fitoquímicos/farmacologia , Urticaceae , Acetatos/química , Linhagem Celular Tumoral , Glucose/metabolismo , Homeostase/efeitos dos fármacos , Humanos , Hipoglicemiantes/química , Fígado/metabolismo , Fenóis/análise , Fenóis/farmacologia , Compostos Fitoquímicos/análise , Casca de Planta/química , Raízes de Plantas/química , Solventes/química
4.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1953-1959, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342726

RESUMO

In this study,mouse models of benign prostatic hyperplasia induced by subcutaneous injection of testosterone propionate was used to investigate the therapeutic effect and mechanism of Urtica hyperborean( UW) extracts on prostate hyperplasia in mice. The effects of UW extracts on prostate index,serum epidermal growth factor( EGF) and dihydrotestosterone( DHT) in model mice were observed,and the EGF and anti-apoptotic factor( Bcl-2) mRNA expression levels were detected as well as pathological changes in prostate tissue. The results showed that the ethyl acetate extraction and alcohol soluble fraction of the UW could significantly reduce the prostate index,reduce the serum DHT and EGF levels( P<0. 01),and significantly decrease the EGF and Bcl-2 mRNA expression( P<0. 01),significantly improved the morphological structure of prostate tissue. The above results confirmed that ethyl acetate extract and alcohol-soluble parts of UW have a good preventive effect on mice prostatic hyperplasia model,and its mechanism may be to reduce androgen levels by regulating polypeptide growth factors and/or inhibiting cell hyperproliferation and promoting apoptosis. This study laid the foundation for the further research on UW.


Assuntos
Medicina Tradicional Tibetana , Extratos Vegetais/farmacologia , Hiperplasia Prostática/tratamento farmacológico , Urticaceae/química , Animais , Di-Hidrotestosterona/sangue , Fator de Crescimento Epidérmico/sangue , Masculino , Camundongos , Hiperplasia Prostática/induzido quimicamente , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Propionato de Testosterona
5.
Zhongguo Zhong Yao Za Zhi ; 44(8): 1607-1614, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-31090325

RESUMO

This paper deals with the application of ultra-performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-ESI-Q-TOF-MS/MS) method to rapidly determine and analyze the chemical constituents of methanol extract of Urtica hyperborea. We employed UPLC YMC-Triart C18(2. 1 mm×100 mm,1. 9 µm) column to UPLC analysis with acetonitrile-water(containing 0. 4% formic acid) in gradient as mobile phase. The flow rate was 0. 3 m L·min-1 gradient elution and column temperature was 30℃; the injection volume was 4 µL. ESI ion source was used to ensure the data collected in anegative ion mode. The chemical components of U. hyperborea were identified through retention time,exact relative molecular mass,cleavage fragments of MS/MS and reported data.The results indicated that a total of 31 compounds were identified,including 8 flavonoids,14 phenolic compounds,8 phenylpropanoids(4 coumarins and 4 lignans),and 1 steroidal compound,13 of which were confirmed by comparison. The UPLC-ESI-Q-TOF-MS/MS method could rapid identify the chemical components of U. hyperborea. The above compounds were discovered in U. hyperborea for the first time,which could provide theoretical foundation for further research on the basis of the pharmacodynamics of U. hyperborea.


Assuntos
Medicamentos de Ervas Chinesas/química , Compostos Fitoquímicos/análise , Extratos Vegetais/análise , Urticaceae/química , Cromatografia Líquida de Alta Pressão , Flavonoides , Lignanas , Fenóis , Espectrometria de Massas em Tandem
6.
Mol Phylogenet Evol ; 137: 190-199, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31102687

RESUMO

The tribe Urticeae (Urticaceae), popularly known as Nettles, include 12 genera and ca. 200 species, constituting a diverse and cosmopolitan plant clade centered in tropical Asia, Africa, and South America. The global distribution of this clade makes it an excellent group to test hypotheses regarding the processes underlying tropical intercontinental disjunctions. More specifically, it allows us to test whether current distribution patterns resulted from recent transoceanic long-distance dispersal or ancient vicariance after boreotropical migration. We reconstructed the phylogeny of Nettles with the nuclear ITS and four plastid DNA regions (rbcL, trnL-F, matK and rpl14-rpl36) using Bayesian inference and maximum parsimony approaches. We inferred divergence times using a Bayesian uncorrelated lognormal relaxed molecular clock model and ancestral areas using the divergence-extinction-cladogenesis (DEC) model. Our results indicate a tropical Asian origin for the tribe during the late Paleocene. Migration events to Eurasia, South America and Africa occurred mainly during the Oligocene and Miocene. However, several long-distance dispersal events, including dispersals from Asia to Hawaii or Australasia, were inferred to have occurred from the Miocene onwards. The fleshy fruits and winged diaspores of several taxa are suited for long-distance dispersal.


Assuntos
Dispersão de Sementes/fisiologia , Urticaceae/fisiologia , Ásia , Teorema de Bayes , Filogenia , Filogeografia , Fatores de Tempo , Clima Tropical
7.
Am J Bot ; 106(3): 489-506, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30875436

RESUMO

PREMISE OF THE STUDY: This study details the unusual synorganization of the staminate flower in wind-pollinated urticalean rosids to add the missing pieces that complete the puzzle of the explosive mechanism of pollen release in this group. METHODS: Flower buds and flowers were analyzed using light and scanning electron microscopy. KEY RESULTS: The pistillode, stamens, and sepals form a floral apparatus that explosively releases pollen to be carried by the wind. The anthers dehisce when the stamens are still inflexed on the floral bud and are enveloped by the sepals and supported by an inflated pistillode. The distension of the filaments presses the pistillode, which decreases the pressure exerted on the anthers by releasing the air accumulated internally through its apical orifice. The extended filaments and the dehiscent free anthers move rapidly outward from the center of the flower. This movement of the filaments is then blocked by the robust basally united sepals, which causes a rapid inversion of the anther position, thus hurling the pollen grains far from the flower. The pollen grains are released grouped by the mucilage produced in high quantity in the cells found in all floral organs. CONCLUSIONS: The anatomical structure of the pistillode and the finding of mucilaginous cells are the main features that help in the understanding the explosive mechanism of pollen release in urticalean rosids. The pistillode can be considered an exaptation because it was evolved later to provide a new role in the plant, optimizing male fitness.


Assuntos
Cannabaceae/anatomia & histologia , Flores/anatomia & histologia , Moraceae/anatomia & histologia , Polinização , Urticaceae/anatomia & histologia , Cannabaceae/fisiologia , Flores/fisiologia , Moraceae/fisiologia , Urticaceae/fisiologia , Vento
8.
Planta Med ; 85(6): 496-502, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30791057

RESUMO

Two new phenanthroquinolizidine alkaloids (1: and 2: ) and a new piperidine derivative (3: ) were isolated from the leaves of Pilea aff. martinii together with 3 known alkaloids: julandine (4: ), cryptopleurine (5: ), and 1,3,6,6-tetramethyl-5,6,7,8-tetrahydro-isoquinolin-8-one (6: ). Their structures were determined by spectral data analyses including mass spectrometry and 2-dimensional nuclear magnetic resonance data. The absolute configurations of 1: -3: were established by comparison of their experimental circular dichroism data with the calculated electronic circular dichroism spectra. The isolated compounds were evaluated for their cytotoxicity against 4 cancer cell lines: KB (mouth epidermal carcinoma cells), HepG-2 (human liver hepatocellular carcinoma cells), LU-1 (human lung adenocarcinoma cells), and MCF-7 (human breast cancer cells). The new phenanthroquinolizidine pileamartine D (2: ) showed strong and selective proliferation inhibition toward KB and HepG-2 cells with IC50 values of 25 and 27 nM, respectively. Pileamartine C (1: ), julandine (4: ), and cryptopleurine (5: ) exhibited cytotoxicity against 4 tested cancer cell lines with IC50 values less than 1 µM.


Assuntos
Alcaloides/isolamento & purificação , Citotoxinas/isolamento & purificação , Folhas de Planta/química , Urticaceae , Linhagem Celular Tumoral/efeitos dos fármacos , Dicroísmo Circular , Células Hep G2/efeitos dos fármacos , Humanos , Células MCF-7/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Urticaceae/química
9.
J Asian Nat Prod Res ; 21(11): 1068-1074, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30693804

RESUMO

Phytochemical investigation of Urtica fissa seed led to the isolation of a new secolignan (1) and a new glycoalkaloid (2), together with 16 known compounds (3-18). The subsequent active evaluation indicated that two lignans (1, 12), two ceramides (3, 4), and the glycoalkaloid (2) possessed the significant cytotoxicity. They could obviously inhibit the proliferation of tumor cells HeLa and CCRF-CEM cells, with IC50 values as low as 1.5 µM.


Assuntos
Lignanas , Urticaceae , Linhagem Celular Tumoral , Estrutura Molecular , Extratos Vegetais , Sementes
10.
J Asian Nat Prod Res ; 21(2): 165-170, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29224377

RESUMO

Two new secolignans, 3,4-trans-3-hydroxymethyl-4-[bis(4-hydroxy-3- methoxyphenyl)methyl]butyrolactone (1) and 3,4-trans-3-hydroxymethyl-4- [bis(3,4-dimethoxyphenyl)methyl]butyrolactone (2) have been isolated from the roots of Urtica fissa E.Pritz. Their structures were determined on the basis of spectroscopic methods, especially 1H NMR, 13C NMR, 2D NMR, and HR-ESI-MS. The inhibitory effects on N1 and N2, two subtypes of neuraminidases (NAs), of these two compounds were assayed.


Assuntos
Lignanas/química , Raízes de Plantas/química , Urticaceae/química , Estrutura Molecular
11.
Mol Phylogenet Evol ; 132: 251-264, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30503949

RESUMO

Elatostema s.s. (Urticaceae) comprises approximately 500 species of herbs and subshrubs distributed in tropical and subtropical Asia, Australasia, and Africa. The delimitation of Elatostema s.s. and the closely related genera Elatostematoides, Pellionia, and Procris has long been problematic because of the large number of taxa and presumed homoplasy among diagnostic morphological characters. In the present study, we refer to these four genera together as Elatostema s.l. To evaluate the circumscription of Elatostema s.l. and its generic and subgeneric classification, we conducted phylogenetic analyses of DNA sequence data from the internal transcribed spacer of the nuclear genome (nrITS) and two markers from the plastid genome (psbA-trnH and psbM-trnD) for 126 taxa, representing 88 species of Elatostema s.s., four of Elatostematoides, nine of Pellionia, and five of Procris. Ten selected morphological characters were investigated using ancestral state reconstructions. Our results show that Elatostema s.l. can be divided into three well-supported and morphologically distinct genera: Procris, Elatostematoides, and Elatostema sensu auct. The results of our molecular phylogeny suggest four strongly supported clades within this newly defined Elatostema s.a.: core Elatostema, Pellionia, Weddellia, and an as yet undescribed clade African Elatostema. Homoplasy among the morphological characters used in this study makes it impossible to circumscribe genera using synapomorphies, but combined suites of characters do enable the morphological diagnosis of Elatostema s.a., Elatostematoides, and Procris.


Assuntos
Urticaceae/classificação , Teorema de Bayes , Núcleo Celular/genética , Ecossistema , Genoma de Planta , Filogenia , Plastídeos/genética , Urticaceae/anatomia & histologia , Urticaceae/genética
12.
J Asian Nat Prod Res ; 21(6): 516-521, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29623730

RESUMO

Two new lignans named neourticol A (1) and neourticol B (2), together with seven known compounds (3-9), were isolated from Urticae Fissae Herba, a folk medicine for rheumatism arthritis in China. The active evaluation results showed that 1 and 2 possessed the potent anti-complement and anti-inflammatory activities.


Assuntos
Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Lignanas/química , Lignanas/farmacologia , Urticaceae/química , Animais , China , Medicina Tradicional Chinesa , Camundongos , Estrutura Molecular , Óxido Nítrico/antagonistas & inibidores , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Células RAW 264.7 , Fator de Necrose Tumoral alfa/metabolismo
13.
J Ethnopharmacol ; 231: 179-186, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30453051

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Debregeasia salicifolia Rendle (DS) is used traditionally for the treatment of several diseases including; urinary system diseases, bone fractures, boils, diarrhea accompanied with blood, carbuncles, pimples, dermatitis, skin rash, eczema, and tumors. Additionally, it is used as an antiseptic and antifungal agent. AIM OF THE STUDY: To document up to date information regarding D. salicifolia and link the traditional uses with its reported pharmacological evidences. The review covers taxonomy, distribution, traditional uses, active phytochemical constituents and pharmacological activities for DS. MATERIALS AND METHODS: The electronic databases such as Google Scholar, E-Resource Portal of Imam Abdulrahman bin Faisal University, Scopus, PubMed, Springer Link, etc. as well as textbooks were used to gather all relevant information about DS. RESULTS: Ethnomedicinal studies revealed a wide use of DS for various traditional purposes in the south Asian community. The uses for diarrhea, cancer, urinary complaints and skin diseases were supported by pharmacological studies. In-vitro pharmacological studies confirmed its therapeutic activities e.g. antimicrobial, anticancer, and antioxidant. Moreover, analgesic and anti-diarrheal activities were also established in animal's in-vivo activities. Despite enormous literature regarding DS, the main focus of research and interest was antimicrobial and cytotoxic activities. Quantitative and qualitative phytochemical analysis proved the presence of various classes of phytochemicals in DS extract; triterpenes, phytosterols, tannins, flavonoids and anthraquinone. CONCLUSION: Though most of the traditional uses for the plant were supported by in-vitro and in-vivo pharmacological studies, however lack of clinical trials and shortage of in-depth mechanistic in-vivo studies was noted in the review. Hence, more elaborative in-vivo studies with quality clinical trials may be focused in order to confirm the safety and efficacy of reported activities in humans which will help explore the plant's therapeutic potentials.


Assuntos
Medicina Tradicional , Fitoterapia , Urticaceae , Animais , Etnobotânica , Etnofarmacologia , Humanos , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/uso terapêutico , Preparações de Plantas/química , Preparações de Plantas/farmacologia , Preparações de Plantas/uso terapêutico
14.
ACS Appl Mater Interfaces ; 10(44): 38574-38580, 2018 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-30335944

RESUMO

Controlling mesoscale organization in thick films of electroactive polymers is crucial for studying and optimizing charge and ion transport in these disordered materials. Conventional approaches focus on directing long-range polymer aggregation and/or crystallization during film formation by using interfaces, flow and/or shear forces. Here, we describe an alternative method that takes advantage of naturally textured biological substrates and vapor-coating to structure thick-conjugated polymer films. Reactive vapor-coating is a technique that enables in situ synthesis of doped conjugated polymers inside a reduced-pressure reactor. Reactive vapor deposition conformally coats the surface of plant matter, such as leaves and flower petals, with conducting polymer films while leaving these living substrates undamaged. Importantly, the intricate surface features of plant matter are faultlessly reproduced in the coating, effectively creating thick, high-surface-area, electrochemically active conducting polymer electrodes on plant matter. A microstructured, 10 µm thick film of p-doped poly(3,4-ethylenedioxythiophene) on a pilea involucrata leaf acts as an all-polymer pseudocapacitor with a higher areal capacitance (142 mF/cm2) than an analogous film on a planar plastic substrate lacking microstructure (50 mF/cm2). Taken together, reactive vapor deposition and microstructured plant matter present a unique combination of processing technique and substrate than can yield a diverse library of controllably microstructured electronic polymer films.


Assuntos
Eletrônica , Transporte de Íons , Polímeros/química , Urticaceae/química , Compostos Bicíclicos Heterocíclicos com Pontes/química , Cristalização , Técnicas Eletroquímicas , Folhas de Planta/química , Resistência ao Cisalhamento , Especificidade por Substrato , Propriedades de Superfície
15.
J Nat Prod ; 81(10): 2169-2176, 2018 10 26.
Artigo em Inglês | MEDLINE | ID: mdl-30336025

RESUMO

Four new Δ12 ursene-type pentacyclic triterpenes containing the trans-feruloyl moiety (1-4), along with ursolic acid (5), were isolated from a Myrianthus arboreus root bark ethanol extract, after bioassay-guided subfractionation of its hexane fraction. The structures of 1-4 were established on the basis of the results of standard spectroscopic analytical methods (IR, HRESIMS, GC-MS, 1D and 2D NMR). The compounds 3ß- O- trans-feruloyl-2α,19α-dihydroxyurs-12-en-28-oic acid (1), 2α-acetoxy-3ß- O- trans-feruloyl-19α-hydroxyurs-12-en-28-oic acid (3), and 5 were determined to decrease the activity of hepatocellular glucose-6-phosphatase (G6Pase) and to activate glycogen synthase (GS). Their action on G6Pase activity implicated both Akt and AMPK activation. In addition, these compounds were determined to stimulate GS via the phosphorylation of glycogen synthase kinase-3. Compound 3 showed the most potent effect in modulating glucose homeostasis in liver cells. This is the first comprehensive report on novel phytochemical components of the root bark extract of M. arboreus based on the isolation of the principles responsible for its antidiabetic effects.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/farmacologia , Triterpenos Pentacíclicos/farmacologia , Casca de Planta/química , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Urticaceae/química , Linhagem Celular , Ativação Enzimática/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Glucose/metabolismo , Glucose-6-Fosfatase/antagonistas & inibidores , Glicogênio Sintase/metabolismo , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Humanos , Estrutura Molecular
16.
Acta Biol Hung ; 69(3): 300-312, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30257584

RESUMO

Copper cause oxidative damage in plant cells, and plant extracts are the sources of free radical scavengers. We tested the hypothesis that whether Corchorus olitorius (jute) and Urtica pilulifera (Roman nettle) seed extract treatments of germinated seeds affect copper induced oxidative and genotoxic damage or antioxidant response in tomato. Seedlings were exposed to toxic copper concentration (30 ppm) for 7 days. In one experimental group (treatment 1), extract (100 µg mL-1) was added to media. In the other group (treatment 2), tomato seeds were pre-soaked by the extract (100 µg mL-1) prior to germination and copper application. Malondialdehyde and endogenous H2O2 levels in the groups treated with extract and copper were significantly lower than that of the untreated groups. Pre-soaking seeds with the nettle extract solution significantly enhanced catalase activity under unstressed condition. Jute treatment also enhanced catalase activity under copper stress. Ascorbate peroxidase activity remained at unstressed level in copper treated groups. Extract treatments significantly decreased copper induced DNA damage in root nuclei. Jute seed extract contained salicylic acid and quercetin which can be correlated with the evoked effects. We demonstrated protective effect of plant extract treatments against copper stress of tomato seedlings prior to germination or during seedling development.


Assuntos
Cobre/toxicidade , Corchorus/química , Lycopersicon esculentum/efeitos dos fármacos , Extratos Vegetais/farmacologia , Urticaceae/química , Ensaio Cometa , Dano ao DNA/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química
17.
Plant Physiol Biochem ; 132: 72-79, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30176430

RESUMO

Pilea sinofasciata is a promising phytoextraction material to remove excess phosphorus (P) from manure-impacted soil. However, little information is available on its physiological response to animal manure treatments. Here, P accumulation, tissue P fractions and acid phosphatase activity were investigated in a mining ecotype (ME) and a non-mining ecotype (NME) of P. sinofasciata at different poultry manure (PM) treatments (0, 25, 50, 75, 100 and 125 g kg-1). Biomass and P accumulation of the ME increased up to 50 g kg-1, after which they significantly decreased; while P accumulation of the NME increased up to 100 g kg-1. But, shoot and root P accumulation of the ME were significantly higher than those of the NME at all PM treatments, showing 1.13-2.92 and 1.11-2.89 times higher values, respectively. Inorganic P and nucleic P dominated in tissues of both ecotypes. Besides, the ME maintained higher concentrations of inorganic P and ester P in leaves and ester P, nucleic P and residual P in roots than the NME. Acid phosphatase activity in leaves and roots increased by increasing PM treatments, except in root at 125 g kg-1. Acid phosphatase activity in leaves of the ME was positively correlated with concentrations of inorganic P, ester P and nucleic P, while that of the NME only correlated with inorganic P concentration. Probably, the optimized P fractions allocation and higher tissue acid phosphatase allow the ME to grow well and efficiently accumulate P in PM-impacted soil.


Assuntos
Fosfatase Ácida/metabolismo , Esterco/análise , Fósforo/metabolismo , Solo/química , Urticaceae/metabolismo , Animais , Biomassa , Ecótipo , Mineração , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Brotos de Planta/metabolismo , Aves Domésticas , Urticaceae/crescimento & desenvolvimento
18.
Colloids Surf B Biointerfaces ; 172: 244-253, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30173091

RESUMO

Despite of great attention concerned on Ni ferrite nanostructures in bioapplications, little is known about the toxicity of these NPs at the cellular and molecular levels. U87 (human primary glioblastoma) and SH-SY5Y (human neuroblastoma) cells treated with various concentration of well-characterized magnetic nickel ferrite nanoparticles, exposed to frequency magnetic field (FMF) and their response was studied. Ferromagnetic nanocrystalline nickel ferrite (NiFe2O4) powder that characterized by XRD, TEM, SEM, FT-IR, nanosizer, and VSM techniques were prepared by a hydrothermal method in the presence of Urtica plant extract as a green precursor that acts both as reducing and capping agent. Owing to the exceptional properties of green alkalinized agent such as minor toxicity, higher biodegradability, high active surface and environment compatibility, we used the green alkalinized agent (Utrica) to prepared nanostructures for the first time. According to the obtained results, the FMF exposure caused an increase in cell death in neural cell types 48 h after treatment. MNPs indicated dose-dependent cytotoxicity but the amount of cell death per cell in the absence of MFM for SH-SY5Y cells was more than in U87 cells. On the other hand, cell death induced by FMF exposure was observed specifically in SH-SY5Y cells. Nevertheless, it is essential to perform more investigations to find the exact related mechanisms. Imatinib showed dose-dependent antiproliferative effects in all three prostate cancer cell lines.


Assuntos
Compostos Férricos/química , Química Verde/métodos , Campos Magnéticos , Nanopartículas de Magnetita/química , Neurônios/citologia , Níquel/química , Urticaceae/química , Morte Celular , Linhagem Celular Tumoral , Forma Celular , Sobrevivência Celular , Humanos , Nanopartículas de Magnetita/ultraestrutura , Neurônios/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
19.
Ecol Lett ; 21(10): 1515-1529, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30133154

RESUMO

Dispersal is a fundamental ecological process, yet demonstrating the occurrence and importance of long-distance dispersal (LDD) remains difficult, having rarely been examined for widespread, non-coastal plants. To address this issue, we integrated phylogenetic, molecular dating, biogeographical, ecological, seed biology and oceanographic data for the inland Urticaceae. We found that Urticaceae originated in Eurasia c. 69 Ma, followed by ≥ 92 LDD events between landmasses. Under experimental conditions, seeds of many Urticaceae floated for > 220 days, and remained viable after 10 months in seawater, long enough for most detected LDD events, according to oceanographic current modelling. Ecological traits analyses indicated that preferences for disturbed habitats might facilitate LDD. Nearly half of all LDD events involved dioecious taxa, so population establishment in dioecious Urticaceae requires multiple seeds, or occasional selfing. Our work shows that seawater LDD played an important role in shaping the geographical distributions of Urticaceae, providing empirical evidence for Darwin's transoceanic dispersal hypothesis.


Assuntos
Urticaceae , Ecologia , Ecossistema , Filogenia , Sementes
20.
Molecules ; 23(7)2018 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-29987208

RESUMO

Nettles (genus Urtica, family Urticaceae) are of considerable interest as preservatives in foods for both human and animal consumption. They have also been used for centuries in traditional medicine. This paper reviews the properties of nettles that make them suitable for wider applications in the food and pharmaceutical industries. Nettles contain a significant number of biologically-active compounds. For example, the leaves are rich sources of terpenoids, carotenoids and fatty acids, as well as of various essential amino acids, chlorophyll, vitamins, tannins, carbohydrates, sterols, polysaccharides, isolectins and minerals. Extracts from the aerial parts of nettles are rich sources of polyphenols, while the roots contain oleanol acid, sterols and steryl glycosides. Due to the variety of phytochemicals and their proportions they contain, nettles show noticeable activity against both Gram-positive and Gram-negative bacteria. These properties make nettles suitable for a range of possible applications, including functional food, dietary supplements and pharmacological formulations. Despite these benefits, the nettle is still an underestimated plant source. This paper provides a unique overview of the latest research on nettle plants focusing on the possibilities for transforming a common weed into a commercial plant with a wide range of applications. Special attention is paid to the antimicrobial activity of the active compounds in nettles and to possible uses of these valuable plants in food and feed formulations.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Compostos Fitoquímicos/farmacologia , Urticaceae/química , Animais , Antibacterianos/química , Antioxidantes/química , Suplementos Nutricionais , Alimento Funcional , Compostos Fitoquímicos/química , Componentes Aéreos da Planta/química , Folhas de Planta/química , Raízes de Plantas/química
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