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1.
Mem Inst Oswaldo Cruz ; 114: e190198, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596309

RESUMO

BACKGROUND: In Brazil the implementation of the Sentinel Surveillance System of Influenza began in 2000. Central public health laboratories use reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for diagnosis of respiratory viruses, but this protocol identifies only specific targets, resulted in inconclusive diagnosis for many samples. Thus, high-throughput sequencing (HTS) would be complementary method in the identification of pathogens in inconclusive samples for RT-qPCR or other specific detection protocols. OBJECTIVES: This study aimed to detect unidentified viruses using HTS approach in negative samples of nasopharynx/tracheal secretions by the standard RT-qPCR collected in the Federal District, Brazil. METHODS: Nucleic acids were extracted from samples collected in winter period of 2016 and subjected to HTS. The results were confirmed by the multiplex PR21 RT-qPCR, which identifies 21 respiratory pathogens. FINDINGS: The main viruses identified by HTS were of families Herpesviridae, Coronaviridae, Parvoviridae and Picornaviridae, with the emphasis on rhinoviruses. The presence of respiratory viruses in the samples was confirmed by the PR21 multiplex RT-qPCR. Coronavirus, enterovirus, bocavirus and rhinovirus were found by multiplex RT-qPCR as well as by HTS analyses. MAIN CONCLUSIONS: Wide virus diversity was found by different methodologies and high frequency of rhinovirus occurrence was confirmed in population in winter, showing its relevance for public health.


Assuntos
Coronaviridae/isolamento & purificação , Herpesviridae/isolamento & purificação , Nasofaringe/virologia , Parvoviridae/isolamento & purificação , Picornaviridae/isolamento & purificação , Traqueia/virologia , Coronaviridae/classificação , Coronaviridae/genética , DNA Viral/genética , Herpesviridae/classificação , Herpesviridae/genética , Humanos , Parvoviridae/classificação , Parvoviridae/genética , Picornaviridae/classificação , Picornaviridae/genética , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
2.
Mol Cell Probes ; 48: 101448, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31521579

RESUMO

Amdoparvoviruses infect carnivore species, including mink, raccoon dog, fox, skunk, and red panda. Amdoparvovirus infection is a major cause of morbidity and mortality in farmed minks. Here, we developed a direct TaqMan qPCR assay for detection and quantification of carnivore amdoparvoviruses by using three primers and one probe based on the conserved VP2 gene. The detection limit for Aleutian mink disease virus (AMDV) and Raccoon dog and arctic fox amdoparvovirus (RFAV) were 4.06 × 101 copies/µl and 2.93 × 101 copies/µl, respectively. Both intra- and inter-assay variability were less than 2%. Among 74 carnivore samples, the positive rates for amdoparvoviruses were 62.2% (46/74) by direct TaqMan qPCR, while only 40.5% (30/74) by SYBR Green I qPCR. This result suggests that the direct TaqMan qPCR was more sensitive than the SYBR Green I qPCR. Additionally, the direct TaqMan qPCR is a rapid and sensitive method for liquid samples at microliter level as the assay employed the direct alkaline lysis method to obtain viral DNA and, therefore, eliminated the cumbersome steps in extracting DNA. Overall, the direct TaqMan qPCR assay possessed high specificity, sensitivity, and reproducibility, indicating that it can be used as a powerful tool for detection and quantification of various carnivore amdoparvoviruses in epidemiological and pathogenesis studies.


Assuntos
Vírus da Doença Aleutiana do Vison/genética , Parvoviridae/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Animais , Primers do DNA/genética , DNA Viral/genética , Cães , Raposas/virologia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise de Sequência de DNA/métodos
3.
Biologicals ; 61: 1-7, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31447377

RESUMO

Horses are often used as blood donors for commercial horse serum (HS) production and to manufacture biologicals. HS is an alternative for fetal bovine serum (FBS) used as a supplement for cell culture and vaccine production. Furthermore, HS is also frequently obtained in order to produce antisera toxins and pathogens. The advent of high-throughput sequencing (HTS) has promoted changes in virus detection, since previous knowledge of targets is not required. Thus, the present study aimed to describe the virome of five different batches of commercial HS from New Zealand (three batches) and Brazil and the United States (one batch each) using HTS. Each HS pool were processed and sequenced using an Illumina MiSeq platform. Sequences-related to viruses belonging to the Flaviviridae, Herpesviridae, and Parvoviridae families were detected. Particularly, equine hepacivirus (EqHV), equine pegivirus (EPgV), and Theiler's disease-associated virus (TDAV) were more frequent found in the batches analyzed. The presence of viral genomes in cell culture sera illustrates that these commercial sera can contain a mixture of different viruses and, therefore, can be regarded as potentially infectious for susceptible hosts. Moreover, the innocuity of commercial HS is important for the efficiency and security of diagnostics and the production of biological products.


Assuntos
Flaviviridae/genética , Genoma Viral , Herpesviridae/genética , Cavalos/virologia , Parvoviridae/genética , Soro/virologia , Animais , Meios de Cultura , Flaviviridae/classificação , Herpesviridae/classificação , Cavalos/sangue , Parvoviridae/classificação
4.
Infect Genet Evol ; 75: 104013, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31446136

RESUMO

Bufavirus (BuV), a recent discovery virus of the family Parvoviridae, has been suggested to be a potential causative agent of diarrhea in humans. The prevalence of BuV has been demonstrated mostly in fecal specimens of diarrheic patients. Little is known about the presence of BuV in the environmental water. The aim of the present study was to conduct a surveillance for BuV in the environmental water in Thailand. A total of 125 water samples were collected during November 2016 and July 2018 from six different areas in Chiang Mai city, Thailand. Water samples were concentrated and extracted to obtain BuV genomic DNA. The BuV was screened by amplification of NS1 region using nested-PCR. The detected BuV was further characterized by amplification of VP2 region. The NS1 and VP2 genes were sequenced and analyzed phylogenetically. The BuV strain (CMW88/18) detected in this study belonged to BuV1 with the prevalence of 0.8%. The CMW88/18 strain was most closely related to human BuV1 strains reported previously worldwide suggesting contamination of BuV in the environmental water could be a potential source of infection in human.


Assuntos
Genoma Viral , Genômica , Parvoviridae/classificação , Parvoviridae/genética , Microbiologia da Água , Diarreia/epidemiologia , Diarreia/virologia , Genômica/métodos , Humanos , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia , Filogenia , Vigilância em Saúde Pública , Tailândia/epidemiologia
5.
J Gen Virol ; 100(3): 367-368, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30672729

RESUMO

Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, single-stranded DNA genomes of 4-6 kb. Viruses in two subfamilies, the Parvovirinae and Densovirinae, are distinguished primarily by their respective ability to infect vertebrates (including humans) versus invertebrates. Being genetically limited, most parvoviruses require actively dividing host cells and are host and/or tissue specific. Some cause diseases, which range from subclinical to lethal. A few require co-infection with helper viruses from other families. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the Parvoviridae, which is available at www.ictv.global/report/parvoviridae.


Assuntos
Infecções por Parvoviridae/virologia , Parvoviridae/classificação , Filogenia , Animais , Genoma Viral , Humanos , Parvoviridae/genética , Parvoviridae/isolamento & purificação , Parvoviridae/ultraestrutura , Virologia/organização & administração
6.
Vet Microbiol ; 228: 246-251, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30593374

RESUMO

A novel protoparvovirus species was identified in domestic cats. The virus was distantly related to the well-known feline (feline panleukopenia virus) and canine (canine parvovirus type 2) parvoviruses, sharing low nucleotide identities in the capsid protein 2 (less than 43%). The virus was genetically similar (100% at the nucleotide level) to a newly identified canine protoparvovirus, genetically related to human bufaviruses. The feline bufavirus appeared as a common element of the feline virome, especially in juvenile cats, with an overall prevalence of 9.2%. The virus was more common in respiratory samples (9.5%-12.2%) than in enteric samples of cats (2.2%). The role of bufaviruses in the etiology of feline respiratory disease complex, either as a primary or a secondary agents, should be defined.


Assuntos
Doenças do Gato/virologia , Infecções por Parvoviridae/veterinária , Parvoviridae/isolamento & purificação , Infecções Respiratórias/veterinária , Animais , Proteínas do Capsídeo/genética , Doenças do Gato/epidemiologia , Gatos , Vírus da Panleucopenia Felina/genética , Parvoviridae/genética , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia , Parvovirus Canino/genética , Filogenia , Prevalência , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/virologia
7.
Vet J ; 232: 57-64, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29428093

RESUMO

A number of novel viruses have been associated with canine gastroenteritis in recent years, from viral families as diverse as Caliciviridae and Picornaviridae to Parvoviridae and Circoviridae. The ability of many of these viruses to cause disease is uncertain, but epidemiological studies are continually adding to our knowledge of these potential pathogens. This review presents a summary of the latest research and current understanding of novel viruses associated with canine gastroenteritis.


Assuntos
Doenças do Cão/virologia , Gastroenterite/veterinária , Animais , Caliciviridae/isolamento & purificação , Circoviridae/isolamento & purificação , Coronaviridae/isolamento & purificação , Cães , Fezes/virologia , Gastroenterite/epidemiologia , Gastroenterite/virologia , Parvoviridae/isolamento & purificação , Picornaviridae/isolamento & purificação , Rotavirus/isolamento & purificação , Viroses/epidemiologia , Viroses/veterinária , Viroses/virologia
8.
Viruses ; 10(1)2018 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-29300333

RESUMO

Bufavirus strain 1 (BuV1), a member of the Protoparvovirus genus of the Parvoviridae, was first isolated from fecal samples of children with acute diarrhea in Burkina Faso. Since this initial discovery, BuVs have been isolated in several countries, including Finland, the Netherlands, and Bhutan, in pediatric patients exhibiting similar symptoms. Towards their characterization, the structures of virus-like particles of BuV1, BuV2, and BuV3, the current known genotypes, have been determined by cryo-electron microscopy and image reconstruction to 2.84, 3.79, and 3.25 Å, respectively. The BuVs, 65-73% identical in amino acid sequence, conserve the major viral protein, VP2, structure and general capsid surface features of parvoviruses. These include a core ß-barrel (ßB-ßI), α-helix A, and large surface loops inserted between these elements in VP2. The capsid contains depressions at the icosahedral 2-fold and around the 5-fold axes, and has three separated protrusions surrounding the 3-fold axes. Structure comparison among the BuVs and to available parvovirus structures revealed capsid surface variations and capsid 3-fold protrusions that depart from the single pinwheel arrangement of the animal protoparvoviruses. These structures provide a platform to begin the molecular characterization of these potentially pathogenic viruses.


Assuntos
Microscopia Crioeletrônica , Processamento de Imagem Assistida por Computador , Parvoviridae/ultraestrutura , Sequência de Aminoácidos , Capsídeo/química , Capsídeo/metabolismo , Proteínas do Capsídeo/química , Microscopia Crioeletrônica/métodos , Humanos , Imageamento Tridimensional , Modelos Moleculares , Parvoviridae/genética , Parvoviridae/isolamento & purificação , Parvoviridae/metabolismo , Sorogrupo
9.
J Wildl Dis ; 54(1): 186-188, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-28820299

RESUMO

: Aleutian disease virus (ADV) and closely related (ADV-like) viruses are Parvoviridae members (genus Amdoparvovirus) that primarily infect farmed mustelids and have been detected in humans and free-ranging Carnivora from North America. We describe ADV-like/ Amdoparvovirus sp. infection in four free-ranging striped skunks ( Mephitis mephitis) from the Midwestern US.


Assuntos
Mephitidae/virologia , Infecções por Parvoviridae/veterinária , Parvoviridae/isolamento & purificação , Animais , Meio-Oeste dos Estados Unidos/epidemiologia , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia
10.
Viruses ; 9(11)2017 11 22.
Artigo em Inglês | MEDLINE | ID: mdl-29165368

RESUMO

Next-generation sequencing and metagenomics have revolutionized the discovery of novel viruses. In recent years, three novel protoparvoviruses have been discovered in fecal samples of humans: bufavirus (BuV) in 2012, tusavirus (TuV) in 2014, and cutavirus (CuV) in 2016. BuV has since been studied the most, disclosing three genotypes that also represent serotypes. Besides one nasal sample, BuV DNA has been found exclusively in diarrheal feces, but not in non-diarrheal feces, suggesting a causal relationship. According to both geno- and seroprevalences, BuV appears to be the most common of the three novel protoparvoviruses, whereas TuV DNA has been found in only a single fecal sample, with antibody detection being equally rare. Moreover, the TuV sequence is closer to those of non-human protoparvoviruses, and so the evidence of TuV being a human virus is thus far insufficient. Interestingly, besides in feces, CuV has also been detected in skin biopsies of patients with cutaneous T-cell lymphoma and a patient with melanoma, while all other skin samples have tested PCR negative. Even if preliminary disease associations exist, the full etiological roles of these viruses in human disease are yet to be resolved.


Assuntos
Doenças Transmissíveis Emergentes/virologia , Infecções por Parvoviridae/virologia , Parvoviridae/genética , Parvoviridae/isolamento & purificação , DNA Viral , Diarreia/virologia , Fezes/virologia , Gastroenterite/virologia , Genoma Viral , Genótipo , Humanos , Linfoma Cutâneo de Células T/virologia , Metagenômica , Parvoviridae/classificação , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/transmissão , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Sorogrupo , Pele/virologia
11.
Virol J ; 13: 133, 2016 07 29.
Artigo em Inglês | MEDLINE | ID: mdl-27473724

RESUMO

Chapparvovirus, a recently determined new genus in the family Parvoviridae, can infect many species of animals including bats, chickens, and pigs. Here, using viral metagenomics method, we identified a novel Chapparvovirus from feces of wild rats and designated it as rat parvovirus 2 (RPV2). The nearly complete genome of RPV2 is 4222-nt long and includes two ORFs encoding a 654-aa nonstructural protein 1 (NS1) and a 472-aa capsid protein (VP), respectively. Phylogenetic analysis over the amino acid sequence of the NS1 showed that RPV2 clustered with Eidolon helvum parvovirus 2 (EHPV2), porcine parvovirus 7 (PPV7), and turkey parvovirus 1 (TP1), forming a separate clade. Sequence analysis indicated that the NS1 protein of RPV2 shared the highest amino acid sequence identity (51 %) with that of EHPV2. According to the genetic distance-based criteria, RPV2 identified here belongs to a novel species of Chapparvovirus.


Assuntos
Infecções por Parvoviridae/veterinária , Parvoviridae/isolamento & purificação , Doenças dos Roedores/virologia , Animais , Animais Selvagens/virologia , Galinhas , China , Fezes/virologia , Genoma Viral , Fases de Leitura Aberta , Parvoviridae/classificação , Parvoviridae/genética , Parvoviridae/metabolismo , Infecções por Parvoviridae/virologia , Filogenia , Ratos , Suínos , Proteínas Virais/genética
12.
PLoS One ; 11(3): e0151921, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26986573

RESUMO

The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals.


Assuntos
Otárias/virologia , Metagenômica , Vírus/genética , Anelloviridae/genética , Animais , Bacteriófagos/genética , Sequência de Bases , Fezes/virologia , Genoma Viral/genética , Biblioteca Genômica , Herpesviridae/genética , Dados de Sequência Molecular , Parvoviridae/genética , Filogenia , Picobirnavirus/genética , Picornaviridae/genética , Rotavirus/genética , Sapovirus/genética
13.
Acta Biomater ; 35: 206-14, 2016 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-26911883

RESUMO

UNLABELLED: Virus-like particles (VLPs) are the product of the self-assembly, either in vivo or in vitro, of structural components of viral capsids. These particles are excellent scaffolds for surface display of biomolecules that can be used in vaccine development and tissue-specific drug delivery. Surface engineering of VLPs requires structural stability and chemical reactivity. Herein, we report the enhanced assembly, colloidal stabilization and fluorescent labeling of primate erythroparvovirus 1 (PE1V), generally referred to as parvovirus B19. In vitro assembly of the VP2 protein of PE1V produces VLPs, which are prone to flocculate and hence undergo limited chemical modification by thiol-specific reagents like the fluorogenic monobromobimane (mBBr). We determined that the addition of 0.2M l-arginine during the assembly process produced an increased yield of soluble VLPs with good dispersion stability. Fluorescent labeling of VLPs suspended in phosphate buffered saline (PBS) added with 0.2M l-Arg was achieved in significantly shorter times than the flocculated VLPs assembled in only PBS buffer. Finally, to demonstrate the potential application of this approach, mBBr-labeled VLPs were successfully used to tag human hepatoma HepG2 cells. This new method for assembly and labeling PE1V VLPs eases its applications and provides insights on the manipulation of this biomaterial for further developments. STATEMENT OF SIGNIFICANCE: Application of virus-derived biomaterials sometimes requires surface modification for diverse purposes, including enhanced cell-specific interaction, the inclusion of luminescent probes for bioimaging, or the incorporation of catalytic properties for the production of enzyme nanocarriers. In this research, we reported for the first time the colloidal stabilization of the primate erythroparvovirus 1 (PE1V) virus-like particles (VLPs). Also, we report the chemical modification of the natural Cys residues located on the surface of these VLPs with a fluorescent probe, as well as its application for tagging hepatoma cells in vitro. Keeping in mind that PE1V is a human pathogen, virus-host interactions already exist in human cells, and they can be exploited for therapeutic and research aims. This study will impact on the speed in which the scientific community will be able to manipulate PE1V VLPs for diverse purposes. Additionally, this study may provide insights on the colloidal properties of these VLPs as well as in the effect of different protein additives used for protein stabilization.


Assuntos
Coloides/química , Parvoviridae/química , Engenharia de Proteínas/métodos , Vírion/química , Animais , Arginina/farmacologia , Compostos Bicíclicos com Pontes/metabolismo , Centrifugação , Cristalografia por Raios X , Endocitose , Filtração , Fluorescência , Glicerol/farmacologia , Células Hep G2 , Humanos , Concentração de Íons de Hidrogênio , Modelos Moleculares , Primatas , Compostos de Sulfidrila/metabolismo , Propriedades de Superfície , Proteínas Virais/química , Proteínas Virais/metabolismo , Vírion/efeitos dos fármacos , Vírion/ultraestrutura
14.
Transbound Emerg Dis ; 63(4): 460-4, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25382194

RESUMO

Parvoviruses represent the most important infectious agents that are responsible for severe to fatal disease in carnivores. This study reports the results of a 10-year molecular survey conducted on carnivores in Bulgaria (n = 344), including 262 dogs and 19 cats with gastroenteritis, and 57 hunted wild carnivores. Real-time polymerase chain reaction (qPCR), followed by virus characterization by minor groove binder (MGB) probe assays, detected 216 parvovirus positive dogs with a predominance of canine parvovirus type 2a (CPV-2a, 79.17%) over CPV-2b (18.52%) and CPV-2c (2.31%). Rottweilers and German shepherds were the most frequent breeds among CPV-positive pedigree dogs (n = 96). Eighteen cats were found to shed parvoviruses in their faeces, with most strains being characterized as FPLV (n = 17), although a single specimen tested positive for CPV-2a. Only two wild carnivores were parvovirus positive, a wolf (Canis lupus) and a red fox (Vulpes vulpes), both being infected by CPV-2a strains.


Assuntos
Carnívoros/virologia , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/veterinária , Animais , Bulgária/epidemiologia , Gatos , Cães , Fezes/virologia , Parvoviridae/classificação , Parvoviridae/genética , Reação em Cadeia da Polimerase em Tempo Real
15.
Vet J ; 206(2): 131-5, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26383859

RESUMO

Bocaparvovirus is a newly established genus within the family Parvoviridae and has been identified as a possible cause of enteric, respiratory, reproductive/neonatal and neurological disease in humans and several animal species. In this study, metagenomic analysis was used to identify and characterise a novel bocaparvovirus in the faeces of rabbits with enteric disease. To assess the prevalence of the novel virus, rectal swabs and faecal samples obtained from rabbits with and without diarrhoea were screened with a specific PCR assay. The complete genome sequence of the novel parvovirus was reconstructed. The virus was distantly related to other bocaparvoviruses; the three ORFs shared 53%, 53% and 50% nucleotide identity, respectively, to homologous genes of porcine bocaparvoviruses. The virus was detected in 8/29 (28%) and 16/95 (17%) samples of rabbits with and without diarrhoea, respectively. Sequencing of the capsid protein fragment targeted by the diagnostic PCR identified two distinct bocaparvovirus populations/sub-types, with 91.7-94.5% nucleotide identity to each other. Including these novel parvoviruses in diagnostic algorithms of rabbit diseases might help inform their potential pathogenic role and impact on rabbit production and the virological profiles of laboratory rabbits.


Assuntos
Infecções por Parvoviridae/veterinária , Parvoviridae , Coelhos , Animais , Genoma Viral , Parvoviridae/genética , Infecções por Parvoviridae/virologia , Filogenia , Cultura de Vírus
16.
Arch Virol ; 160(10): 2617-21, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26239342

RESUMO

Waterfowl parvoviruses are divided into Muscovy duck parvoviruses (MDPVs) and goose parvoviruses (GPVs). Phylogenetic analysis based on structural gene nucleotide sequences showed that the strains of three GPVs (DY, PT and D strains) and two MDPVs (GX5 and SAAH-SHNH) are closely related and formed one cluster. Recombination analysis showed that recombination between GPV-GDFsh and MDPV-89384/FRANCE strains led to five recombinant strains: GPV-DY, GPV-PT, GPV-D, MDPV-GX5 and MDPV-SAAH-SHNH. The recombinant event was confirmed using the Simplot program and phylogenetic analysis. This is the first comprehensive investigation of recombination between MDPV and GPV structural genes.


Assuntos
Infecções por Parvoviridae/veterinária , Parvoviridae/genética , Doenças das Aves Domésticas/virologia , Recombinação Genética , Proteínas Estruturais Virais/genética , Animais , Patos , França , Gansos , Dados de Sequência Molecular , Parvoviridae/classificação , Parvoviridae/isolamento & purificação , Infecções por Parvoviridae/virologia , Filogenia
17.
Virol J ; 12: 114, 2015 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-26239432

RESUMO

The parvoviruses are widely spread in many species and are among the smallest DNA animal viruses. The parvovirus is composed of a single strand molecule of DNA wrapped into an icosahedral capsid. In a viral infection, the massy capsid participates in the entire viral infection process, which is summarized in this review. The capsid protein VP1 is primarily responsible for the infectivity of the virus, and the nuclear localization signal (NLS) of the VP1 serves as a guide to assist the viral genome in locating the nucleus. The dominant protein VP2 provides an "anti-receptor", which interacts with the cellular receptor and leads to the further internalization of virus, and, the N-terminal of VP2 also cooperates with the VP1 to prompt the process of nucleus translocation. Additionally, a cleavage protein VP3 is a part of the capsid, which exists only in several members of the parvovirus family; however, the function of this cleavage protein remains to be fully determined. Parvoviruses can suffer from the extreme environmental conditions such as low pH, or even escape from the recognition of pattern recognition receptors (PRRs), due to the protection of the stable capsid, which is thought to be an immune escape mechanism. The applications of the capsid proteins to the screening and the treatment of diseases are also discussed. The processes of viral infection should be noted, because understanding the virus-host interactions will contribute to the development of therapeutic vaccines.


Assuntos
Proteínas do Capsídeo/metabolismo , Infecções por Parvoviridae/virologia , Parvoviridae/metabolismo , Animais , Capsídeo/metabolismo , Proteínas do Capsídeo/genética , Núcleo Celular/metabolismo , Genoma Viral , Humanos , Fases de Leitura Aberta , Parvoviridae/genética , Transporte Proteico
18.
Arch Virol ; 160(8): 2111-5, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26085285

RESUMO

To investigate the possible role of recombination in the evolution of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) in Taiwan, we analyzed a potentially significant recombination event that occurred only in GPV by comparing thirteen complete sequences of the capsid gene VP2 of GPV and MDPV. The recombination event occurred between GPV strain 06-0239 as the minor parent and strains 99-0808 as the major parent, which resulted in the GPV recombinant V325/TW03. GPV V325/TW03 is likely to represent a new genotype among the Taiwanese GPV strains. This represents the first evidence that intergenotype recombination within the VP2 gene cluster contributes to the genetic diversity of the VP2 genes of Taiwanese GPV field strains.


Assuntos
Proteínas do Capsídeo/genética , Gansos/virologia , Infecções por Parvoviridae/veterinária , Parvoviridae/genética , Doenças das Aves Domésticas/virologia , Recombinação Genética , Animais , Dados de Sequência Molecular , Parvoviridae/classificação , Parvoviridae/isolamento & purificação , Infecções por Parvoviridae/virologia , Filogenia , Taiwan
19.
J Invertebr Pathol ; 128: 73-9, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25968106

RESUMO

Hepatopancreatic parvo-like virus (HPV) has been reported from a variety of shrimp species around the world, including Australia, and thought to impact negatively on production, but until now there was scant information available on variation of HPV over time, ponds and shrimp lineages or families, information that could be used to manage or reduce virus levels. Here we report HPV copy number estimated using qPCR from 1500 individual shrimp sampled over three years and encompassing 91 ponds, 21 breeding groups or lineages and 40 families. HPV copy number variation between ponds was used by farm management as a criterion to choose prospective broodstock (candidates were taken from low HPV ponds). Despite such choice, HPV levels in farmed animals were not reduced from 2011 to 2013. Accordingly, the hypothesis that HPV levels can be reduced over time simply by considering average HPV levels in ponds alone is rejected. Different lines of shrimp within the same farm had different HPV levels, but as lines were raised separately, the line differences could be due to either genetic or environmental differences, the latter including possible different rearing effects and differences in vertical transmission. There were large (up to 2-3 LOG fold) differences of HPV levels between families bred and grown together contemporaneously, and the heritability for HPV copy number was estimated to be moderate to large (0.40 ± 0.13). Apart from genetic differences, differences of vertical transmission from dams may contribute to the between family differences, in any case we postulate that selection between families could be an effective method to reduce HPV levels. HPV levels were not genetically correlated with performance traits such as body weight or length, so selection for HPV level should not adversely affect production characteristics. This is the first evidence for an aquacultured species that viral levels, as opposed to survival/resistance to viruses, may have a substantial host genetic component. The heritability reported here for virus copy number was higher that most heritabilities reported for survival to specific pathogens such as white spot, raising the general postulate that selection for virus copy number may be more effective and repeatable than selection for survival to pathogen challenge.


Assuntos
Parvoviridae , Penaeidae/virologia , Tanques/microbiologia , Microbiologia da Água , Animais , Aquicultura/normas , Reação em Cadeia da Polimerase , Frutos do Mar/virologia
20.
Arch Virol ; 160(7): 1781-4, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25951971

RESUMO

Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27%) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear.


Assuntos
Diarreia/virologia , Fezes/virologia , Infecções por Parvoviridae/virologia , Parvoviridae/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Diarreia/epidemiologia , Feminino , Genótipo , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Parvoviridae/classificação , Parvoviridae/genética , Infecções por Parvoviridae/epidemiologia , Filogenia , Prevalência , Tailândia/epidemiologia , Adulto Jovem
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