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1.
Indian J Med Res ; 147(3): 239-247, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29923512

RESUMO

Erythrovirus B19 (B19V) is one of the erythroviruses known to be pathogenic in humans. B19V is classified into three distinct genotypes; 1, 2 and 3, differing from each other by 2-13 per cent. Genotype 1 consists of the prototype B19V isolates, genotype 2 comprises the A6, LaLi and their related isolates while genotype 3 includes the V9- and V9-related isolates. The classification of genotype 1 into two subtypes (1A and 1B) and genotype 3 into two subtypes (3a and 3b) with an estimated nucleotide difference of about 5 per cent has been done. Predominance of genotype 1 across all the continents is seen followed by genotypes 2 and 3. There are no disease-specific genotypes. All the three genotypes have been found in symptomatic as well as asymptomatic individuals and have been reported from several countries across the world. The prevalence of genotype 2 in older populations and its absence from current circulation in Northern Europe has also been reported. The present review focuses on geographic distribution and association of genotypes of B19V with different clinical manifestations.


Assuntos
Infecções por Parvoviridae/epidemiologia , Parvovirus B19 Humano/genética , DNA Viral , Erythrovirus , Variação Genética , Genótipo , Humanos , Infecções por Parvoviridae/genética , Parvovirus B19 Humano/patogenicidade
2.
J Med Virol ; 88(4): 588-95, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26369294

RESUMO

B19V infection is common during childhood. It is self-limited in healthy individuals, but is often associated with transient aplastic crisis in children with sickle cell disease. The aim of this study was to estimate the prevalence and incidence of B19V infection in children with sickle cell disease screened by the Newborn Screening Program of Minas Gerais, Brazil, and followed-up at Fundação Hemominas. Serum or plasma samples from 278 patients were tested for anti-B19V IgG and IgM using commercial ELISA and for viral DNA using in-house real-time PCR assays; 127 negative-children were retested about 1 year later. The median age of children at first testing was 5.9 years (0.8-12.3). The estimated prevalence of B19V was 29.5 % (95%CI 24.1-34.9 %). The incidence of B19V in those 127 negative-children was 18.2 cases/100 patient-years. All DNA-positive samples were identified as genotype 1, except one sample, in which both genotypes 1 and 3 were identified. It was observed that the higher the child's age, the higher the probability of B19V infection. The analysis of clinical and hematological data showed a significant association of B19V infection with transient aplastic crisis and acute splenic sequestration, higher frequency of transfusions, and higher rate of hospitalization, but not with acute chest syndrome or stroke. These results emphasize the impact of B19V infection on the course of sickle cell disease. Strategies to prevent and monitor B19V infection in children with sickle cell disease should be considered to diminish its morbidity in this susceptible population.


Assuntos
Anemia Falciforme/complicações , Erythrovirus/isolamento & purificação , Infecções por Parvoviridae/epidemiologia , Adolescente , Fatores Etários , Anticorpos Antivirais/sangue , Brasil/epidemiologia , Criança , Pré-Escolar , DNA Viral/sangue , Ensaio de Imunoadsorção Enzimática , Erythrovirus/classificação , Erythrovirus/genética , Feminino , Variação Genética , Genótipo , Humanos , Imunoglobulina G/sangue , Incidência , Lactente , Masculino , Prevalência , Reação em Cadeia da Polimerase em Tempo Real , Estudos Retrospectivos , Fatores de Risco
3.
J Med Virol ; 86(9): 1614-20, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24265024

RESUMO

Human erythrovirus B19 (EVB19) is a small, pathogenic DNA virus that has been associated with a wide range of illnesses. The primary site of replication is in bone marrow-derived erythroid progenitor cells, but EVB19 DNA has been detected in a wide range of organs. Recently, studies have linked EVB19 to thyroid cancers and other thyroid diseases. Previous studies from multiple laboratories have detected EVB19 capsid proteins in Graves' disease, Hashimoto's thyroiditis, and thyroid cancer tissues. Data on viral gene expression and mechanism of infection in the thyroid are lacking. To investigate EVB19 infection and persistence in the thyroid, previously archived adult and pediatric tissue sections were examined for EVB19 DNA, RNA, and capsid proteins, as well as EVB19 receptor P-antigen and co-receptor α5ß1 integrin. EVB19 DNA and protein were detected in a majority of tissues examined (87% and 68%, respectively). Detection was similar in adult and pediatric samples. Quantification of viral genomes revealed no significant difference in the amount of viral DNA in benign, cancerous, or metastatic thyroid tissues. EVB19 capsid RNA was detected in 67% of the tissues examined, confirming at least low-level viral gene expression. Immunohistochemical staining for P-antigen and α5ß1 detected the receptor and co-receptor most frequently on normal thyroid epithelial cells. EVB19 capsid staining could be detected in tumors lacking viral receptors. These results suggest that normal thyroid epithelial cells are the initial target for EVB19 infection in the thyroid and allow for continued persistence in both normal and cancerous thyroid tissues.


Assuntos
Adenoma/virologia , Carcinoma Papilar/virologia , Erythrovirus/genética , Infecções por Parvoviridae/virologia , Glândula Tireoide/virologia , Neoplasias da Glândula Tireoide/virologia , Adenoma/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteínas do Capsídeo/metabolismo , Carcinoma Papilar/metabolismo , Carcinoma Papilar/secundário , Criança , DNA Viral/genética , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Infecções por Parvoviridae/metabolismo , Infecções por Parvoviridae/patologia , RNA Viral/genética , Glândula Tireoide/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Neoplasias da Glândula Tireoide/patologia , Adulto Jovem
4.
J Med Virol ; 85(8): 1414-9, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23765778

RESUMO

Environmental factors, such as viruses, are thought to contribute to the development of thyroid autoimmunity. Erythrovirus B19 (EVB19) is suspected to be involved in Hashimoto's thyroiditis, but no direct evidence is available concerning the role of EVB19 infection in Graves' disease. The objective of this study was to investigate whether the presence of EVB19 is more frequent in thyroidectomy specimens of patients undergoing thyroidectomy for Graves' disease (cases) than for multinodular thyroid (controls). Serum and thyroidectomy specimens were prospectively collected from 64 patients referred for total thyroidectomy over a 5-year period (2007-2011) and were investigated retrospectively and blindly for circulating EVB19 DNA by q-PCR (Qiagen), and for EVB19 thyrocyte infection by immunochemistry (VP2-Antibody, Dako). EVB19 serology was also determined. General clinical and laboratory data were collected. Twenty patients were referred for Graves' disease and 44 patients were referred for non-autoimmune multinodular thyroid. Patients with thyroid cancer were excluded. Ten percent of Graves' disease patients and 27.7% of control patients had positive staining of thyrocytes for EVB19 antibodies (ns). EVB19-positive and EVB19-negative cases did not differ. EVB19-positive controls were older than EVB19-negative controls (mean age: 57.5 [35-74] vs. 45 [28-80] years, P=0.03) No case of acute EVB19 infection was identified. EVB19-positive serology was more frequent in controls than in Graves' disease patients (88% vs. 45%, P<0.0001). EVB19 was detected in thyrocytes, but not more frequently in Graves' disease patients than in controls. Further studies are needed to determine the role of EVB19 infection in thyroid diseases.


Assuntos
Erythrovirus/isolamento & purificação , Doença de Graves/virologia , Timo/virologia , Tireoidectomia , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antivirais/sangue , Estudos de Casos e Controles , Doença de Graves/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Prospectivos , Estudos Retrospectivos , Soro/virologia , Adulto Jovem
5.
Enferm Infecc Microbiol Clin ; 30 Suppl 2: 70-5, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22542038

RESUMO

Viral infections are a major cause of morbidity and even mortality in solid organ transplant recipients. This article reviews key aspects of infections in solid organ transplant recipients from respiratory viruses, such as influenza, polyomavirus, erythrovirus B19 and measles.


Assuntos
Transplante de Órgãos/efeitos adversos , Viroses/etiologia , Erythrovirus , Humanos , Influenza Humana/diagnóstico , Influenza Humana/tratamento farmacológico , Influenza Humana/terapia , Sarampo/diagnóstico , Sarampo/etiologia , Sarampo/terapia , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/etiologia , Infecções por Parvoviridae/terapia , Viroses/diagnóstico , Viroses/terapia
6.
Methods Mol Biol ; 665: 213-31, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21116804

RESUMO

Parvovirus B19 is a single-stranded DNA virus which causes severe disease in immunocompromised patients and foetal loss in pregnant women. It is classified as an Erythrovirus and this genus also comprises two related viral genotypes (so-called LaLi/A6 (genotype 2) and V9 (genotype 3)) which appear to be immunologically indistinguishable from Parvovirus B19. Serological and nucleic acid test (NAT) systems to detect Parvovirus B19-mediated infection are commercially available; however, some NAT systems are genotype-specific. International standard preparations of Parvovirus B19 IgG and DNA have been produced for assay standardisation purposes, and to ensure consistency of assay manufacture and performance. Immunological assays, such as B-cell ELISpot, T-cell stimulation, and cytokine detection can also be used to confirm exposure to Parvovirus B19. Immunohistochemical techniques, employing commercially available monoclonal antibodies, are used to localise the virus in infected tissue and Parvovirus B19 viral antigen can also be detected in serum and plasma using antigen-specific ELISA. NAT systems have also been described to detect newly identified parvoviruses such as human bocavirus (HBoV), PARV4, and PARV5, although absolute confirmation of clinical diseases associated with these agents is required. This chapter describes the current status of detection systems for all the aforementioned parvoviruses, with particular emphasis on Erythrovirus detection by serological, NAT, and immunological approaches.


Assuntos
Erythrovirus/genética , Bocavirus Humano/genética , Parvovirinae/isolamento & purificação , Parvovirus B19 Humano/genética , Anticorpos Antivirais/isolamento & purificação , Linfócitos B/química , Primers do DNA , DNA de Cadeia Simples/genética , DNA Viral/análise , DNA Viral/genética , Ensaio de Imunoadsorção Enzimática , Erythrovirus/imunologia , Feminino , Genótipo , Humanos , Parvovirinae/genética , Parvovirus B19 Humano/imunologia , Parvovirus B19 Humano/fisiologia , Reação em Cadeia da Polimerase/métodos , Gravidez , Sensibilidade e Especificidade
7.
PLoS One ; 5(12): e15113, 2010 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-21151930

RESUMO

The transcription profile of chipmunk parvovirus (ChpPV), a tentative member of the genus Erythrovirus in the subfamily Parvovirinae of the family Parvoviridae, was characterized by transfecting a nearly full-length genome. We found that it is unique from the profiles of human parvovirus B19 and simian parvovirus, the members in the genus Erythrovirus so far characterized, in that the small RNA transcripts were not processed for encoding small non-structural proteins. However, like the large non-structural protein NS1 of the human parvovirus B19, the ChpPV NS1 is a potent inducer of apoptosis. Further phylogenetic analysis of ChpPV with other parvoviruses in the subfamily Parvovirinae indicates that ChpPV is distinct from the members in genus Erythrovirus. Thus, we conclude that ChpPV may represent a new genus in the family Parvoviridae.


Assuntos
Erythrovirus/genética , Infecções por Parvoviridae/genética , Parvoviridae/genética , Animais , Apoptose , Células COS , Chlorocebus aethiops , Erythrovirus/classificação , Humanos , Parvoviridae/classificação , Filogenia , Plasmídeos/metabolismo , Sciuridae , Transcrição Genética
8.
Clin Infect Dis ; 50(1): 115-8, 2010 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-19951110

RESUMO

We investigated the role that erythroviruses (parvovirus B19 and erythrovirus genotypes 2 and 3) play in the lives of immunosuppressed HIV-infected patients with chronic anemia. We screened the serum samples of 428 patients by specific ultrasensitive real-time polymerase chain reaction assay. Sixteen patients had circulating DNA, with no apparent clinical impact. Erythrovirus-associated anemia is an extremely rare event in HIV-infected patients.


Assuntos
Anemia/virologia , Erythrovirus/fisiologia , Infecções por HIV/virologia , Parvovirus B19 Humano/fisiologia , Replicação Viral , Adulto , Anemia/sangue , Anemia/complicações , Doença Crônica , Estudos de Coortes , DNA Viral/sangue , Erythrovirus/isolamento & purificação , Feminino , Infecções por HIV/sangue , Infecções por HIV/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Infecções por Parvoviridae/sangue , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/virologia , Parvovirus B19 Humano/isolamento & purificação , Reação em Cadeia da Polimerase
9.
Transfus Clin Biol ; 16(5-6): 482-8, 2009.
Artigo em Francês | MEDLINE | ID: mdl-19880339

RESUMO

The B19 Parvovirus (B19V) has for a long time been considered as the unique human virus belonging to the genus Erythrovirus. The genetic diversity of B19V isolates has been shown to be very low. The isolation of a variant (V9 strain), with a sequence markedly distinct from that of B19V which led to attributing this classification to this family of viruses. Phylogenetic analysis of sequences of V9-related isolates indicates an organization into three well-individualized genotypes. The B19V infection can be transmitted by transfusion. In immunocompetent recipients, B19V exposure by transfusion is most often inconsequential, since a large proportion is immunized. Such an infection may have serious clinical outcome in not immunized patients with shortened red cell survival, seronegative pregnant women and immunocompromised patients. In cellular products, viral DNA detection is not performed, but a preventive strategy could be discussed for at-risk recipients. Whereas in plasma derivatives, B19V screening is performed with a threshold of 10(4)IU/ml using molecular assays. With recent data of a new classification of three genotypes within human erythrovirus, nucleic acid testing assays would be validated in accordance with the genetic variability, in order to guarantee optimal safety. Recently, a new human parvovirus (PARV4) has been discovered. The consequences on blood transfusion of this blood-borne agent and its pathogenicity are still unknown.


Assuntos
Patógenos Transmitidos pelo Sangue , Erythrovirus/genética , Infecções por Parvoviridae/transmissão , Plasma/virologia , Reação Transfusional , DNA Viral/sangue , Variação Genética , Humanos , Hospedeiro Imunocomprometido/imunologia , Infecções por Parvoviridae/prevenção & controle , Parvovirus B19 Humano/genética
10.
Arch Virol ; 154(10): 1685-7, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19701602

RESUMO

Human parvovirus B19 is the only member of the genus Erythrovirus that causes human disease. Recent findings of several strains with considerable sequence divergence from B19 have suggested a new classification for parvovirus genotypes as 1 (B19), 2 (A-6 and LaLi) and 3 (V9). In their overall DNA sequence, the three genotypes differ by approximately 10%. Here, we report the isolation of a genotype-3-related strain named BR543 during a prospective study conducted in Sao Paulo, Brazil. Analysis of the nearly full-length genome sequence of BR543 indicates that this B19 variant sequence clusters with Gh2768, a strain from Ghana belonging to subtype 3b, and showed mostly synonymous substitutions.


Assuntos
Erythrovirus/genética , Sequência de Bases , Brasil , Variação Genética/genética , Genoma Viral/genética , Genótipo , Humanos , Dados de Sequência Molecular , Infecções por Parvoviridae/virologia , Parvovirus B19 Humano/genética , Filogenia
11.
Med Princ Pract ; 18(4): 339-41, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19494546

RESUMO

OBJECTIVE: To estimate the prevalence of IgG antibodies against B19 virus (B19V) in Makkah and Jeddah, Saudi Arabia. METHODS: B19V-specific IgG antibodies were detected by a commercial indirect enzyme-linked immunosorbent assay in sera of 400 paediatric patients (185 males and 215 females) aged 1-17 years. RESULTS: Of the 400 patients, 80 (20%) had sera positive for B19V-specific IgG. The difference in the prevalence of the antibodies between genders was not statistically significant (p = 0.9). The prevalence of anti-B19V antibodies increased significantly in the age group of 12-17 years as compared to younger patients (37.5 vs. 18% in those aged 1-11 years; p = 0.006). CONCLUSION: This study indicated a high prevalence of IgG antibodies against B19V in paediatric patients with an increase in age.


Assuntos
Anticorpos Antivirais/sangue , Erythrovirus/imunologia , Imunoglobulina G/sangue , Infecções por Parvoviridae/epidemiologia , Adolescente , Distribuição por Idade , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lactente , Masculino , Infecções por Parvoviridae/imunologia , Arábia Saudita/epidemiologia , Estudos Soroepidemiológicos
12.
Pathol Biol (Paris) ; 57(2): 167-74, 2009 Mar.
Artigo em Francês | MEDLINE | ID: mdl-18387751

RESUMO

B19 Parvovirus (B19V) has been considered for a long period of time as the unique human virus belonging to the genus Erythrovirus. The genetic diversity of B19V isolates has been shown to be very low (<2% nucleotide divergence). The isolation of a variant (V9 strain), with a sequence markedly distinct from that of B19V (>13% nucleotide divergence) led to specify the classification of this virus family. Phylogenetic analysis of partial sequences of V9-related isolates combined with Erythrovirus sequences in sequences banks indicates an organization into three well-individualized genotypes. Analysis of the nearly full-length genome sequences show an ancient separation between the three genotypes lineages. Genotype 3 (the most ancient lineage) could have originated in Africa. The functional regions of major proteins are conserved in the three genotypes. The frequency of these genotypes is various according to studies. Genotype 1 is predominant, except in Ghana where all the described isolates were genotype 3. A prospective French study performed between 1999 and 2001 indicated that genotypes 2 and 3 viruses circulated with a significant frequency (10%). Pathogenic properties might not differ according to the genotype.


Assuntos
Erythrovirus/genética , Variação Genética , África/epidemiologia , Antígenos Virais/genética , Erythrovirus/classificação , Erythrovirus/isolamento & purificação , Europa (Continente)/epidemiologia , Genótipo , Humanos , Infecções por Parvoviridae/epidemiologia , Filogenia , Prevalência , Estados Unidos/epidemiologia
14.
J Med Virol ; 80(7): 1243-51, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18461615

RESUMO

Parvovirus B19 (PVB19) is a member of the human erythrovirus family detected frequently in endomyocardial biopsies from patients with dilated cardiomyopathy. Human erythroviruses cluster into three genotypes 1-3 which share a high degree of homology between major structural proteins and may cause indistinguishable infections clinically and serologically. In human cardiac tissue erythrovirus genotypes other than PVB19 have not yet been reported. Three hundred seventeen consecutive patients with symptomatic dilated cardiomyopathy (median left ventricular ejection fraction: 28.6%, range 5-45%) who underwent endomyocardial biopsy for the elucidation of the etiology, were analyzed using a new consensus PCR assay designed for the detection of the three erythrovirus genotype sequences. Endomyocardial biopsies of 151 (47.6%) patients were erythrovirus-positive. Genotype 1 specific sequences were detected in 43/151 (28.5%) of positive biopsy samples, whereas genotype 2-specific sequences so far considered rare in human disease and not yet been described in human heart tissue was identified in 108/151 (71.5%) of virus-positive endomyocardial biopsies with a preference in patients above 50 years of age. In spite of younger age, systolic left ventricular dysfunction of genotype 1-positive patients was significantly reduced as compared to genotype 2-positive patients (24.4+/-10.4% vs. 31.0+/-9.5%, P=0.0001) at the initial presentation. The data show that two genetically distinct erythrovirus variants with a different age distribution are detectable in endomyocardial biopsies of patients with dilated cardiomyopathy. The erythrovirus genotype 2, not described previously in human heart tissue, is highly prevalent in the heart but the less prevalent genotype 1 is associated with more severe disturbed cardiac function.


Assuntos
Cardiomiopatia Dilatada/virologia , Erythrovirus/isolamento & purificação , Coração/virologia , Infecções por Parvoviridae/virologia , Adulto , Idoso , Sequência de Aminoácidos , Sequência de Bases , Proteínas do Capsídeo/química , Erythrovirus/genética , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Prevalência , Alinhamento de Sequência , Carga Viral
15.
J Virol Methods ; 148(1-2): 40-7, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18054089

RESUMO

Variant samples from the three genotypes of erythroviruses have already been detected using sequencing as methodology for analysis. This study aimed to investigate the efficacy of single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assay (HMA) as methodologies to detect human erythrovirus variants, using their VP1 unique region sequences. Clinical samples and plasmids of PVBAUA, A6, LaLi, V9Gh3051, and D91.1 erythrovirus variants as prototypes of the three genotypes were used. SSCP analysis was able to distinguish all divergences among the plasmids, including the two mutation points between LaLi and A6 plasmids that led to distinct electrophoresis mobility patterns. Although HMA analysis was unabled to detect two mutation points between LaLi and A6, it enabled the differentiation among all other plasmids that revealed specific electrophoresis patterns, with high-enough sensibility to detect 1.5% nucleotide substitutions. When 57 clinical samples were analyzed, 33 of them presented an identical pattern to PVBAUA by HMA and SSCP analyses, two of them were sequenced and presented an identical sequence in relation to PVBAUA. Another pattern was found for 21 samples. Among these, two samples were sequenced, revealing one mutation point in relation to PVBAUA, while each one of the three remaining samples presented a distinct pattern, showing two or three mutations in relation to PVBAUA by sequencing. HMA and SSCP analyses were suggested as methodologies suited for detecting genetic mutations of human erythroviruses in developing countries because of their practicability and minor costs for reagents and equipment.


Assuntos
DNA Viral/genética , Erythrovirus/classificação , Erythrovirus/genética , Análise Heteroduplex/métodos , Infecções por Parvoviridae/virologia , Polimorfismo Conformacional de Fita Simples , Adolescente , Adulto , Sequência de Bases , Criança , Pré-Escolar , Erythrovirus/isolamento & purificação , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Filogenia , Mutação Puntual , Sensibilidade e Especificidade , Análise de Sequência de DNA , Proteínas Estruturais Virais/genética
16.
Proc Natl Acad Sci U S A ; 103(19): 7450-3, 2006 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-16651522

RESUMO

Human erythrovirus is a minute, single-stranded DNA virus causing many diseases, including erythema infectiosum, arthropathy, and fetal death. After primary infection, the viral genomes persist in solid tissues. Besides the prototype, virus type 1, two major variants (virus types 2 and 3) have been identified recently, the clinical significance and epidemiology of which are mostly unknown. We examined 523 samples of skin, synovium, tonsil, or liver (birth year range, 1913-2000), and 1,640 sera, by qualitative and quantitative molecular assays for the DNA of human erythroviruses. Virus types 1 and 2 were found in 132 (25%) and 58 (11%) tissues, respectively. DNA of virus type 1 was found in all age groups, whereas that of type 2 was strictly confined to those subjects born before 1973 (P < 0.001). Correspondingly, the sera from the past two decades contained DNA of type 1 but not type 2 or 3. Our data suggest strongly that the newly identified human erythrovirus type 2 as well as the prototype 1 circulated in Northern and Central Europe in equal frequency, more than half a century ago, whereafter type 2 disappeared from circulation. Type 3 never attained wide occurrence in this area during the past > or =70 years. The erythrovirus DNA persistence in human tissues is lifelong and represents a source of information about our past, the Bioportfolio, which, at the individual level, provides a registry of one's infectious encounters, and at the population level, a database for epidemiological and phylogenetic analyses.


Assuntos
Vírus de DNA/genética , Erythrovirus/genética , Variação Genética/genética , Genoma Viral/genética , Expectativa de Vida , Infecções por Parvoviridae/virologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Humanos , Fígado/virologia , Pessoa de Meia-Idade , Infecções por Parvoviridae/sangue , Pele/virologia , Membrana Sinovial/virologia , Fatores de Tempo
17.
Transplantation ; 81(8): 1165-70, 2006 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-16641603

RESUMO

BACKGROUND: Simian parvovirus (SPV) was first isolated from cynomolgus monkeys. Like human parvovirus B19, this virus has a predilection for erythroid cells. During acute SPV infection, clinical signs are usually mild or inapparent, but severe anemia may occur in immunocompromised animals. We report several cases of symptomatic SPV infection in cynomolgus monkeys following heart transplantation. METHODS: Twenty-three consecutive abdominal heterotopic heart transplants were studied. Viremia, measured by dot blot and/or PCR, and SPV-specific antibodies were determined retrospectively. RESULTS: All except one animal were on an immunosuppressive protocol. In all, 48% (11/23) of transplant recipients had viremia with SPV detected at some point after transplant. An additional 22% seroconverted before or after transplant, and were asymptomatic without detectable SPV. Of the 11 acutely viremic animals, five were euthanized because of severe anemia attributed to SPV. The remaining 30% of the transplant recipients did not seroconvert and were asymptomatic. Of seven recipients of donor tissue from seropositive or viremic animals, five became viremic and three died with anemia. No immunosuppressive regimen was implicated in increased susceptibility; the one transplant recipient not treated with immunosuppressive agents died with anemia and acute viremia two weeks after explant of a rejected graft. CONCLUSION: SPV is an important pathogen in surgically manipulated cynomolgus monkeys, particularly with immune compromise. Once introduced into a colony, clinically silent SPV infection could be readily transmitted within the environment. Transmission and disease occur at high frequency with an organ from a PCR-negative, seropositive donor, suggesting that latent virus can be conveyed by the organ.


Assuntos
Anemia/veterinária , Erythrovirus , Transplante de Coração/efeitos adversos , Doenças dos Macacos/epidemiologia , Infecções por Parvoviridae/veterinária , Anemia/etiologia , Animais , Anticorpos Antivirais/sangue , Causas de Morte , Macaca fascicularis , Masculino , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/epidemiologia
18.
J Clin Microbiol ; 44(2): 604-6, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16455922

RESUMO

The presence of erythrovirus infections was investigated by PCR with bone marrow samples of patients with various parvovirus B19-related hematological symptoms. Erythrovirus DNA was found in 17.3% (12/69) of patients. Phylogenetic analysis revealed that five strains cluster with genotype 1, one clusters with genotype 2, and six cluster with genotype 3. Our study is the first to document the presence of the three erythrovirus genotypes in Brazil.


Assuntos
Medula Óssea/virologia , Erythrovirus/classificação , Variação Genética , Doenças Hematológicas/virologia , Infecções por Parvoviridae/virologia , Parvovirus B19 Humano/patogenicidade , Análise de Sequência de DNA , Adolescente , Adulto , Idoso , Brasil , Criança , DNA Viral/análise , DNA Viral/isolamento & purificação , Erythrovirus/genética , Erythrovirus/isolamento & purificação , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular
20.
J Virol ; 78(23): 12929-39, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15542645

RESUMO

Simian parvovirus (SPV) is a member of the genus Erythrovirus and is closely related to the human parvovirus B19. Natural and experimental infection of monkeys with SPV resembles B19 infection of human. We report a detailed characterization of the viral RNAs and proteins generated following transfection of cloned SPV into COS cells and SPV infection of the human erythroid progenitor line UT-7/Epo-S1. SPV and B19 are 50% identical at the nucleotide level, and although their basic transcription and protein expression profiles were generally similar, there were also significant differences. SPV pre-mRNAs contain three introns, compared to two found for B19: an additional intron was found within the capsid-coding region. RNAs in which this intron was spliced were abundant and encoded the SPV 14-kDa protein (analogous to the B19 11-kDa protein), which initiated at an AUG in the exon preceding the third intron. Unlike B19, SPV RNAs were also spliced between the donor of the first intron and the acceptor of the second intron. The third intron was additionally spliced from a portion of these molecules; these mRNAs encoded the 14-kDa protein. A portion was not spliced further and encoded VP2. Like B19, SPV has a polyadenylation signal [AAUAAA (pA)p] in the middle of the genome, which directed efficient polyadenylation of both spliced and unspliced mRNAs (encoding a putative 10-kDa protein, analogous to the B19 7.5-kDa protein, and SPV NS1, respectively). The 14-kDa protein was localized to both in the nucleus and cytoplasm.


Assuntos
Enterovirus Humano B/genética , Erythrovirus/genética , Transcrição Genética , Animais , Northern Blotting , Células COS , Perfilação da Expressão Gênica , Inativação Gênica , Fases de Leitura Aberta , Poliadenilação , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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