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1.
PLoS Negl Trop Dis ; 13(12): e0007915, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31841521

RESUMO

BACKGROUND: Orthohantavirus infection is a neglected global health problem affecting approximately 200,000 people/year, spread by rodent hosts and associated to fatal human diseases, such as hemorrhagic fever with renal syndrome (HFRS) and orthohantavirus cardiopulmonary syndrome (HCPS). Circulation of HFRS-associated orthohantaviruses, such as Seoul, Gou, Amur, Dobrava and Hantaan, are supposed to be restricted to Eurasian countries even though their hosts can be a worldwide distribution. Few confirmed HFRS orthohantavirus infections in humans have been reported in American countries, but due to lower medical awareness of the symptoms of this zoonosis, it could be associated to viral underreporting or to misdiagnosis with several tropical hemorrhagic diseases. Serological evidence of orthohantavirus infections, using enzyme-linked immunosorbent assay for the presence of immunoglobulin M and G against recombinant nucleoprotein protein, remains as an essential assay for viral surveillance. In this study, we aimed to identify in silico immunogenic B-cell linear epitopes present on orthohantavirus nucleoprotein that are exclusive to HFRS-related species. METHODOLOGY/PRINCIPAL FINDINGS: In silico analysis were performed using Seoul orthohantavirus nucleoprotein (SHNP) sequence as a model. Linear B-cell-epitopes on SHNP and its immunogenicity were predicted by BepiPred-2.0 and Vaxijen algorithms, respectively. The conservancy of predicted epitopes was compared with the most clinically relevant HFRS or HCPS-associated orthohantavirus, aiming to identify specific sequences from HFRS-orthohantavirus. Peptide validation was carried out by ELISA using Balb/c mice sera immunized with purified recombinant rSHNP. Peptides cross-reactivity against HCPS orthohantavirus were evaluated using immunized sera from mice injected with recombinant Juquitiba orthohantavirus nucleoprotein (rJHNP). CONCLUSION/SIGNIFICANCE: In silico analysis revealed nine potential immunogenic linear B-cell epitopes from SHNP; among them, SHNP(G72-D110) and SHNP(P251-D264) showed a high degree of sequence conservation among HFRS-related orthohantavirus and were experimentally validated against rSHNP-IMS and negatively validated against rJHNP-IMS. Taken together, we identified and validated two potential antigenic B-cell epitopes on SHNP, which were conserved among HFRS-associated orthohantavirus and could be applied to the development of novel immunodiagnostic tools for orthohantavirus surveillance.


Assuntos
Mapeamento de Epitopos , Epitopos de Linfócito B/imunologia , Vírus Seoul/imunologia , Animais , Antígenos Virais/administração & dosagem , Antígenos Virais/imunologia , Biologia Computacional , Epitopos de Linfócito B/genética , Camundongos Endogâmicos BALB C , Vírus Seoul/genética
2.
PLoS Negl Trop Dis ; 13(9): e0007757, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31545808

RESUMO

Seoul hantavirus (SEOV) has recently raised concern by causing geographic range expansion of hemorrhagic fever with renal syndrome (HFRS). SEOV infections in humans are significantly underestimated worldwide and epidemic dynamics of SEOV-related HFRS are poorly understood because of a lack of field data and empirically validated models. Here, we use mathematical models to examine both intrinsic and extrinsic drivers of disease transmission from animal (the Norway rat) to humans in a SEOV-endemic area in China. We found that rat eradication schemes and vaccination campaigns, but below the local elimination threshold, could diminish the amplitude of the HFRS epidemic but did not modify its seasonality. Models demonstrate population dynamics of the rodent host were insensitive to climate variations in urban settings, while relative humidity had a negative effect on the seasonality in transmission. Our study contributes to a better understanding of the epidemiology of SEOV-related HFRS, demonstrates asynchronies between rodent population dynamics and transmission rate, and identifies potential drivers of the SEOV seasonality.


Assuntos
Febre Hemorrágica com Síndrome Renal/epidemiologia , Febre Hemorrágica com Síndrome Renal/transmissão , Animais , China/epidemiologia , Cidades , Clima , Febre Hemorrágica com Síndrome Renal/prevenção & controle , Humanos , Modelos Teóricos , Controle de Roedores , Roedores/virologia , Estações do Ano , Vírus Seoul , Vacinação
3.
Am J Trop Med Hyg ; 100(5): 1236-1239, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30915955

RESUMO

Hemorrhagic fever with renal syndrome (HFRS) is a febrile disorder caused in Korea by the Hantaan and Seoul viruses. Its characteristic clinical manifestations include fever, hemorrhage, and renal failure, but a primary presentation with acute infectious diarrhea is rare. Owing to decreased urine output and renal function, a 54-year-old patient was transferred to our hospital from a local clinic, where he had been receiving treatment for diarrhea occurring more than 10 times a day. The patient was treated in the Gastroenterology Department at our hospital for acute renal failure secondary to inflammatory diarrhea based on the findings of stool leukocytes. An immunofluorescent antibody assay showed a 4-fold increase in the acute-phase antibody titer to Hantavirus during recovery. A nested reverse transcription polymerase chain reaction (RT-nPCR) assay of plasma yielded negative results, but Hantaan virus positivity was confirmed on an RT-nPCR assay of the buffy coat. Another 60-year-old patient with watery diarrhea was treated conservatively for suspected infectious diarrhea. However, an immunofluorescent antibody assay showed a 4-fold increase in the acute-phase HFRS antibody titer. RT-nPCR using plasma yielded negative results, but Seoul virus was detected on an RT-nPCR buffy coat assay, confirming the diagnosis of HFRS. Hemorrhagic fever with renal syndrome can present with gastrointestinal symptoms such as acute diarrhea alone. This report highlights the importance of considering HFRS in the differential diagnosis of patients with acute diarrhea and the need for additional research on the usefulness of the buffy coat in the PCR diagnosis of HFRS.


Assuntos
Anticorpos Antivirais/sangue , Diarreia/virologia , Febre Hemorrágica com Síndrome Renal/complicações , Febre Hemorrágica com Síndrome Renal/diagnóstico , Doença Aguda , Diagnóstico Diferencial , Diarreia/diagnóstico , Vírus Hantaan/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Vírus Seoul/isolamento & purificação
4.
Can J Vet Res ; 83(1): 75-77, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30670905

RESUMO

Hantavirus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). Heilongjiang Province is experiencing an epidemic of HFRS, the main causative agent is a variant of hantavirus called Seoul virus (SEOV). In this study, the entire genome of one SEOV, the DN2 strain, was sequenced and analyzed. The alignment analysis of the sequences indicated that the DN2 strain shares the highest homology with the SEOV-LYO852 strain. The nucleotide identity is 97.6% for the S segment, 97.7% for the M segment, and 98.0% for the L segment. The corresponding amino acid sequence homologies are 99.1%, 98.9% and 99.8%. The phylogenetic analysis of the segments suggests that the DN2 strain has a high genetic relationship with SEOV strains and no genetic recombination occurs.


Assuntos
Genoma Viral/genética , Febre Hemorrágica com Síndrome Renal/virologia , Vírus Seoul/genética , Animais , China/epidemiologia , Febre Hemorrágica com Síndrome Renal/epidemiologia , Filogenia , RNA Viral/genética , Ratos , Vírus Seoul/classificação , Alinhamento de Sequência , Análise de Sequência , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico
5.
Emerg Infect Dis ; 25(2): 387-388, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30666956

RESUMO

Although Seoul orthohantavirus is the only globally spread hantavirus pathogen, few confirmed human infections with this virus have been reported in Western countries, suggesting lower medical awareness of the milder, transient, and often chameleon-like symptoms of this zoonosis. We describe lesser known clinical and laboratory characteristics to help improve underreporting of this virus.


Assuntos
Febre Hemorrágica com Síndrome Renal/diagnóstico , Febre Hemorrágica com Síndrome Renal/virologia , Vírus Seoul , Humanos , Reação em Cadeia da Polimerase , Testes Sorológicos , Índice de Gravidade de Doença , Avaliação de Sintomas
6.
J Med Virol ; 91(5): 724-730, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30609070

RESUMO

Seoul virus (SEOV) is the etiologic agent of hemorrhagic fever with renal syndrome. It is carried by brown rats (Rattus norvegicus), a commensal rodent that closely cohabitates with humans in urban environments. SEOV has a worldwide distribution, and in Europe, it has been found in rats in UK, France, Sweden, and Belgium, and human cases of SEOV infection have been reported in Germany, UK, France, and Belgium. In the search of hantaviruses in brown rats from the Netherlands, we found both serological and genetic evidence for the presence of SEOV in the local wild rat population. To further decipher the relationship with other SEOV variants globally, the complete genome of SEOV in the Netherlands was recovered. SEOV sequences obtained from three positive rats (captured at close trapping locations at the same time) were found highly similar. Phylogenetic analyses demonstrated that two lineages of SEOV circulate in Europe. Strains from the Netherlands and UK, together with the Baxter strain from US, constitute one of these two, while the second includes strains from Europe and Asia. Our results support a hypothesis of diverse routes of SEOV spread into Europe. These findings, combined with other indications on the expansion of the spatial European range of SEOV, suggest an increased risk of this virus for the public health, highlighting the need for increased surveillance.


Assuntos
Portador Sadio/veterinária , Transmissão de Doença Infecciosa , Vetores de Doenças , Genoma Viral , Febre Hemorrágica com Síndrome Renal/transmissão , Ratos/virologia , Vírus Seoul/isolamento & purificação , Animais , Portador Sadio/virologia , Feminino , Genótipo , Humanos , Masculino , Países Baixos , Vírus Seoul/classificação , Vírus Seoul/genética , Sequenciamento Completo do Genoma
7.
BMC Infect Dis ; 18(1): 578, 2018 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-30445913

RESUMO

BACKGROUND: Seoul virus (SEOV) is a member of hantavirus family, which is transmitted to humans by Rattus rattus and Rattus norvegicus. Diagnosing SEOV infection is difficult because the clinical presentations are often undifferentiated with other viral or bacterial infections and assays to test antibodies seroconversion and RNA detection are not available in resource-limited setting like Indonesia. CASE PRESENTATION: We report two confirmed cases of SEOV infection from Indonesia. Here, we illustrate the clinical presentations, hematology and biochemistry profiles, and outcomes of the two cases. Phylogenetic analysis revealed that SEOV sequences have highest homology to isolates obtained from rodents in Indonesia. CONCLUSIONS: This report highlights the importance of considering SEOV infection in febrile patients with lymphopenia, thrombocytopenia, and elevation of liver enzyme despite the absence of hemorrhagic manifestations and renal syndromes. The public health importance of rodent-borne diseases such as SEOV infection urges an integrated epidemiological surveillance both in humans and rodents in Indonesia.


Assuntos
Febre/diagnóstico , Febre/virologia , Febre Hemorrágica com Síndrome Renal/diagnóstico , Adulto , Animais , Diagnóstico Diferencial , Feminino , Febre Hemorrágica com Síndrome Renal/patologia , Humanos , Indonésia , Masculino , Pessoa de Meia-Idade , Filogenia , RNA Viral/análise , RNA Viral/genética , Ratos , Roedores/virologia , Vírus Seoul/genética , Vírus Seoul/isolamento & purificação
8.
Emerg Infect Dis ; 24(12): 2158-2163, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30067176

RESUMO

Orthohantaviruses are a group of rodentborne viruses with a worldwide distribution. The orthohantavirus Seoul virus (SEOV) can cause hemorrhagic fever with renal syndrome in humans and is distributed worldwide, like its reservoir host, the rat. Cases of SEOV in wild and pet rats have been described in several countries, and human cases have been reported in the United Kingdom, France, Canada, and the United States. In the Netherlands, SEOV has previously been found in wild brown rats. We describe an autochthonous human case of SEOV infection in the Netherlands. This patient had nonspecific clinical symptoms of an orthohantavirus infection (gastrointestinal symptoms and distinct elevation of liver enzymes). Subsequent source investigation revealed 2 potential sources, the patient's feeder rats and a feeder rat farm. At both sources, a high prevalence of SEOV was found in the rats. The virus closely resembled the Cherwell and Turckheim SEOV strains that were previously found in Europe.


Assuntos
Febre Hemorrágica com Síndrome Renal/virologia , Ratos/virologia , Vírus Seoul , Adulto , Animais , Febre Hemorrágica com Síndrome Renal/diagnóstico , Febre Hemorrágica com Síndrome Renal/etiologia , Humanos , Masculino , Países Baixos , Vírus Seoul/classificação , Vírus Seoul/isolamento & purificação
9.
Am J Trop Med Hyg ; 99(2): 470-476, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29869603

RESUMO

Rodent-borne pathogens pose a critical public health threat in urban areas. An epidemiological survey of urban rodents was conducted from 2006 to 2010 at the U.S. Army Garrison (USAG), Seoul, Republic of Korea (ROK), to determine the prevalence of Seoul virus (SEOV), a rodent-borne hantavirus. A total of 1,950 rodents were captured at USAG, Yongsan, near/in 19.4% (234/1,206) of the numbered buildings. Annual mean rodent infestation rates were the highest for food service facilities, e.g., the Dragon Hill Lodge complex (38.0 rodents) and the Hartell House (18.8 rodents). The brown rat, Rattus norvegicus, accounted for 99.4% (1,939/1,950) of all the rodents captured in the urban area, whereas only 0.6% (11/1,950) of the rodents was house mice (Mus musculus). In November 2006, higher numbers of rats captured were likely associated with climatic factors, e.g., rainfall and temperatures as rats sought harborage in and around buildings. Only 4.7% (34/718) of the rodents assayed for hantaviruses was serologically positive for SEOV. A total of 8.8% (3/34) R. norvegicus were positive for SEOV RNA by reverse transcription polymerase chain reaction, of which two SEOV strains were completely sequenced and characterized. The 3' and 5' terminal sequences revealed incomplete complementary genomic configuration. Seoul virus strains Rn10-134 and Rn10-145 formed a monophyletic lineage with the prototype SEOV strain 80-39. Seoul virus Medium segment showed the highest evolutionary rates compared with the Large and Small segments. In conclusion, this report provides significant insights into continued rodent-borne disease surveillance programs that identify hantaviruses for analysis of disease risk assessments and development of mitigation strategies.


Assuntos
Genoma Viral , Instalações Militares , Doenças dos Roedores/epidemiologia , Roedores/virologia , Vírus Seoul/genética , Animais , Genômica , Infecções por Hantavirus/epidemiologia , Camundongos/virologia , Filogenia , Prevalência , RNA Viral/genética , Chuva , Ratos/virologia , República da Coreia/epidemiologia , Vírus Seoul/isolamento & purificação , Análise de Sequência de DNA , Temperatura
11.
MMWR Morb Mortal Wkly Rep ; 67(4): 131-134, 2018 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-29393924

RESUMO

In December 2016, the Wisconsin Department of Health Services (WDHS) notified CDC of a patient hospitalized with fever, leukopenia, elevated transaminases, and proteinuria. The patient owned and operated an in-home rattery, or rat-breeding facility, with approximately 100 Norway rats, primarily bred as pets. A family member developed similar symptoms 4 weeks later, but was not hospitalized. Because both patients were known to have rodent contact, they were tested for hantavirus infections. In January 2017, CDC confirmed recent, acute Seoul virus infection in both patients. An investigation was conducted to identify additional human and rat infections and prevent further transmission. Ultimately, the investigation identified 31 facilities in 11 states with human and/or rat Seoul virus infections; six facilities also reported exchanging rats with Canadian ratteries. Testing of serum samples from 183 persons in the United States and Canada identified 24 (13.1%) with Seoul virus antibodies; three (12.5%) were hospitalized and no deaths occurred. This investigation, including cases described in a previously published report from Tennessee (1), identified the first known transmission of Seoul virus from pet rats to humans in the United States and Canada. Pet rat owners should practice safe rodent handling to prevent Seoul virus infection (2).


Assuntos
Surtos de Doenças , Febre Hemorrágica com Síndrome Renal/epidemiologia , Febre Hemorrágica com Síndrome Renal/veterinária , Doenças dos Roedores/epidemiologia , Vírus Seoul/isolamento & purificação , Animais , Canadá/epidemiologia , Surtos de Doenças/veterinária , Humanos , Propriedade , Ratos , Doenças dos Roedores/virologia , Estados Unidos/epidemiologia
12.
Emerg Infect Dis ; 24(2): 249-257, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29350137

RESUMO

Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR-based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000-2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.


Assuntos
Variação Genética , Febre Hemorrágica com Síndrome Renal/virologia , Reação em Cadeia da Polimerase Multiplex , Vírus Seoul/genética , Animais , Genoma Viral , Saúde Global , Febre Hemorrágica com Síndrome Renal/epidemiologia , Humanos , Filogeografia , RNA Viral/genética , Ratos , República da Coreia/epidemiologia , Estudos Retrospectivos , Estações do Ano , Testes Sorológicos
14.
Epidemiol Infect ; 145(12): 2458-2465, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28724455

RESUMO

Recent cases of acute kidney injury due to Seoul hantavirus infection from exposure to wild or pet fancy rats suggest this infection is increasing in prevalence in the UK. We conducted a seroprevalence study in England to estimate cumulative exposure in at-risk groups with contact with domesticated and wild rats to assess risk and inform public health advice. From October 2013 to June 2014, 844 individual blood samples were collected. Hantavirus seroprevalence amongst the pet fancy rat owner group was 34.1% (95% CI 23·9-45·7%) compared with 3·3% (95% CI 1·6-6·0) in a baseline control group, 2·4% in those with occupational exposure to pet fancy rats (95% CI 0·6-5·9) and 1·7% with occupational exposure to wild rats (95% CI 0·2-5·9). Variation in seroprevalence across groups with different exposure suggests that occupational exposure to pet and wild rats carries a very low risk, if any. However incidence of hantavirus infection among pet fancy rat owners/breeders, whether asymptomatic, undiagnosed mild viral illness or more severe disease may be very common and public health advice needs to be targeted to this at-risk group.


Assuntos
Febre Hemorrágica com Síndrome Renal/epidemiologia , Exposição Ocupacional , Doenças dos Roedores/epidemiologia , Vírus Seoul/isolamento & purificação , Adolescente , Adulto , Animais , Inglaterra/epidemiologia , Febre Hemorrágica com Síndrome Renal/virologia , Humanos , Incidência , Pessoa de Meia-Idade , Animais de Estimação , Prevalência , Ratos , Doenças dos Roedores/virologia , Estudos Soroepidemiológicos , Adulto Jovem
15.
Virol J ; 14(1): 133, 2017 07 18.
Artigo em Inglês | MEDLINE | ID: mdl-28720142

RESUMO

BACKGROUND: Hantaan and Seoul viruses, in the Hantavirus genus, are known to cause hemorrhagic fever with renal syndrome (HFRS). The plaque reduction neutralization test (PRNT), as conventional neutralization test for hantaviruses, is laborious and time-consuming. Alternatives to PRNT for hantaviruses are required. METHODS: In this study, the methods for Hantaan and Seoul viruses serological typing including microneutralization test (MNT), pseudoparticle neutralization test (PPNT) and immunofluorescence assay based on viral glycoproteins (IFA-GP) were developed and compared with PRNT using a panel of 74 sera including 44 convalescent sera of laboratory confirmed HFRS patients and 30 patients sera of non-hantavirus infection. Antibody titres and serotyping obtained with different methods above were analyzed by paired-t, linear correlation, McNemar χ2 and Kappa agreement tests. RESULTS: Antibody titres obtained with MNT50, PPNT50 and IFA-GP were significantly correlated with that obtained with PRNT50 (p < 0.001). GMT determined by PPNT50 was statistically higher than that determined by PRNT50 (p < 0.001), while GMT determined by MNT50 and IFA-GP were equal with (p > 0.05) and less than (p < 0.001) that obtained with PRNT50 respectively. Serotyping obtained with MNT50 and PRNT50, PPNT50 and PRNT50 were highly consistent (p < 0.001), whereas that obtained with IFA-GP and PRNT50 were moderately consistent (p < 0.001). There were no significant differences for serotyping between PRNT50 and MNT50, as well as PRNT50 and PPNT50 (p > 0.05). IFA-GP was less sensitive than PRNT50 and MNT50 for serotyping of hantaviruses infection (p < 0.05). However, for 79.5% (35/44) samples, serotyping determined by IFA-GP and PRNT50 were consistent. CONCLUSIONS: MNT50 and PPNT50 both can be used as simple and rapid alternatives to PRNT50, and MNT50 is more specific while PPNT50 is more sensitive than other assays for neutralizing antibody determination. So far, this work has been the most comprehensive comparison of alternatives to PRNT.


Assuntos
Anticorpos Antivirais/sangue , Vírus Hantaan/imunologia , Vírus Seoul/imunologia , Sorotipagem/métodos , Humanos , Sensibilidade e Especificidade
16.
Virol Sin ; 32(3): 235-244, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28669005

RESUMO

Seoul virus (SEOV), which is predominantly carried by Rattus norvegicus, is one of the major causes of hemorrhagic fever with renal syndrome (HFRS) in China. Hubei province, located in the central south of China, has experienced some of the most severe epidemics of HFRS. To investigate the mitochondrial DNA (mtDNA)-based phylogenetics of wild rats in Hubei, and the relationship with SEOV infection, 664 wild rats were captured from five trapping sites in Hubei from 2000-2009 and 2014-2015. Using reverse-transcription (RT)-PCR, 41 (6.17%) rats were found to be positive for SEOV infection. The SEOV-positive percentage in Yichang was significantly lower than that in other areas. The mtDNA D-loop and cytochrome b (cyt-b) genes of 103 rats were sequenced. Among these animals, 37 were SEOV-positive. The reconstruction of the phylogenetic relationship (based on the complete D-loop and cyt-b sequences) allowed the rats to be categorized into two lineages, R. norvegicus and Rattus nitidus, with the former including the majority of the rats. For both the D-loop and cyt-b genes, 18 haplotypes were identified. The geographic distributions of the different haplotypes were significantly different. There were no significant differences in the SEOVpositive percentages between different haplotypes. There were three sub-lineages for the D-loop, and two for cyt-b. The SEOV-positive percentages for each of the sub-lineages did not significantly differ. This indicates that the SEOV-positive percentage is not related to the mtDNA D-loop or cyt-b haplotype or the sub-lineage of rats from Hubei.


Assuntos
DNA Mitocondrial/genética , Febre Hemorrágica com Síndrome Renal/veterinária , Filogeografia , Ratos/classificação , Ratos/virologia , Doenças dos Roedores/virologia , Vírus Seoul/isolamento & purificação , Animais , China , Citocromos b/genética , DNA Mitocondrial/química , Haplótipos , Febre Hemorrágica com Síndrome Renal/virologia , RNA Viral/genética , RNA Viral/isolamento & purificação , Ratos/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Vírus Seoul/genética , Análise de Sequência de DNA
17.
Viral Immunol ; 30(5): 330-335, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28537534

RESUMO

Hemorrhagic fever with renal syndrome (HFRS) is caused by Hantaan virus (HTNV) and Seoul virus infection in Asia. The clinical manifestation of HFRS is characterized by the rapid loss of renal function (acute kidney injury) and thrombocytopenia. The specific immune mechanisms that cause thrombocytopenia in HFRS are not well described. The growth arrest-specific 6 (Gas6) protein and TAM (Tyro3, Axl and Mer) receptors have been recently shown to play prominent roles in immune regulation, and high plasma levels of Gas6 may predict the severity of diseases. The association of TAM receptors with several autoimmune diseases has been investigated, although the relationship between TAM receptors and these diseases remains unclear in HFRS. Therefore, the aim of this study was to evaluate the clinical significance of Gas6 and TAM receptor expression in HFRS. The concentrations of Gas6 in the plasma from 144 patients and the expression of TAM receptors on monocytes from 117 patients were quantified. The relationship between Gas6 levels and disease course, severity, and clinical parameters was analyzed. We first found that the plasma Gas6 levels were significantly higher in HFRS patients, whereby they were positively correlated with white blood cell counts and negatively correlated with platelet counts. The expression of Tyro3 was increased on monocytes in HFRS patients compared with that in controls. Taken together, our data indicate that elevated plasma Gas6 levels is associated with the severity of disease during HTNV infection in humans, suggesting that Gas6 may play an important role by binding with Tyro3 on monocytes, which will be assessed in future studies.


Assuntos
Febre Hemorrágica com Síndrome Renal/patologia , Peptídeos e Proteínas de Sinalização Intercelular/sangue , Monócitos/química , Plasma/química , Receptores Proteína Tirosina Quinases/análise , Índice de Gravidade de Doença , Adolescente , Adulto , Idoso , Ásia , Feminino , Humanos , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Contagem de Plaquetas , Proteínas Proto-Oncogênicas/análise , Vírus Seoul , Adulto Jovem , c-Mer Tirosina Quinase/análise
18.
Emerg Infect Dis ; 23(6): 973-977, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28368241

RESUMO

We report detection of Seoul virus in 3 patients in France over a 2-year period. These patients accounted for 3 of the 4 Seoul virus infections among 434 hantavirus infections (1.7%) reported during this time. More attention should be given to this virus in Europe where surveillance has been focused mostly on Puumala and Dobrava-Belgrade hantaviruses.


Assuntos
Febre Hemorrágica com Síndrome Renal/diagnóstico , Febre Hemorrágica com Síndrome Renal/epidemiologia , Vírus Seoul , Adulto , Animais , Anticorpos Antivirais , França/epidemiologia , Febre Hemorrágica com Síndrome Renal/virologia , Humanos , Masculino , Ratos , Adulto Jovem
19.
Int J Mol Med ; 38(3): 951-60, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27430149

RESUMO

Hemorrhagic fever with renal syndrome (HFRS) is a severe, viral zoonotic disease which occurs worldwide, particularly in Asia and Europe. In China, the Hantaan virus (HTNV) and the Seoul virus (SEOV) are known to be the most prevalent causative agents of HFRS. Since no protective vaccines or effective treatments are available for human use, accurate and reliable diagnostic methods are essential for disease surveillance. In the present study, the viral loads in cell culture supernatant, infected mice blood and clinical serum samples were quantified using the SYBR­Green I-based reverse transcription-quantitiative polymerase chain reaction (RT-qPCR) assay, which targeted the S gene sequence of the HTNV and SEOV genomes. The cRNA of these two viruses were synthesized as a positive control and 10-fold serially diluted from 1x105 to 1x100 copies/µl. Standard curves were generated by plotting the mean cycle threshold (Ct) values versus copy numbers. The standard curve of HTNV had a correlation coefficient (R2) of 0.994, efficiency of amplification (E) of 101.9%, and the slope of -3.278, whereas that of SEOV had an R2 of 0.993, E of 104.8%, and the slope of -3.212. The minimum detection limit of the RT-qPCR assay for HTNV and SEOV was 101 copies/µl. Two qPCR assays were successfully established for the detection of HTNV and SEOV, respectively. Taken together, these findings demonstrate that using the SYBR­Green I-based RT-qPCR assay, the HTNV and SEOV may be genotyped precisely without cross-reactivity. Furthermore, viral RNA may be detected and quantified in cells, mice and infected individuals, which may be useful in epidemiological studies as well as for early monitoring and further preventative treatment against SEOV and HTNV-induced diseases.


Assuntos
Vírus Hantaan/genética , Febre Hemorrágica com Síndrome Renal/diagnóstico , Compostos Orgânicos/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Vírus Seoul/genética , Animais , Dosagem de Genes , Genoma Viral/genética , Genótipo , Vírus Hantaan/fisiologia , Febre Hemorrágica com Síndrome Renal/sangue , Febre Hemorrágica com Síndrome Renal/virologia , Interações Hospedeiro-Patógeno , Humanos , Camundongos , RNA Complementar/sangue , RNA Complementar/química , RNA Complementar/genética , RNA Viral/sangue , RNA Viral/química , RNA Viral/genética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Vírus Seoul/fisiologia , Especificidade da Espécie
20.
J Wildl Dis ; 52(3): 705-8, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27258409

RESUMO

Hantavirus infections among human populations are linked to the geographic distribution of the host rodents that carry the viruses. To determine the presence and distribution of hantaviruses in the northern region of Xinjiang Uygur Autonomous Region (XUAR), northwestern China, 844 rodents were captured from five locations in four dissimilar habitats during 2010-14 and examined for Hantavirus infection. Hantavirus nucleic acids were firstly detected in the brown rat ( Rattus norvegicus ) from Ürümqi, China, indicating that the Hantavirus was transmitted into Ürümqi in XUAR and circulated by the brown rat. Our results suggest that the brown rat may act as a natural reservoir for the virus in XUAR.


Assuntos
Febre Hemorrágica com Síndrome Renal/veterinária , Doenças dos Roedores/virologia , Vírus Seoul/isolamento & purificação , Animais , China/epidemiologia , Febre Hemorrágica com Síndrome Renal/epidemiologia , Febre Hemorrágica com Síndrome Renal/virologia , Filogenia , Ratos , Doenças dos Roedores/epidemiologia , Vírus Seoul/genética
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