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1.
Ned Tijdschr Tandheelkd ; 127(6): 358-364, 2020 Jun.
Artigo em Holandês | MEDLINE | ID: mdl-32716399

RESUMO

Gingivitis and periodontitis can worsen with reduced immune fitness. Various causes can reduce immune fitness in a host, as a result of which the balance between the host and the microbiome is disturbed. Among others, lifestyle factors, such as stress and smoking, can have a negative influence on immune fitness. An association has been demonstrated between stress and periodontitis and also acute necrotising ulcerative gingivitis or periodontitis. There are indications that neurons are able to secrete pro-inflammatory cytokines and chemokines that worsen chronic inflammatory reactions in the periodontium and compromise immune fitness. In vitro studies show high cortisol levels may contribute to the increased growth of P. gingivalis. Stress as a risk factor for periodontitis and the role of stress as a negative influence on the results of periodontal treatment are difficult to estimate clinically. Nevertheless, attention to and awareness of stress as an aspect of the comprehensive set of risk factors for periodontitis can diminish its negative impact on immune fitness.


Assuntos
Gengivite , Periodontite , Humanos , Inflamação , Periodonto
2.
Acta Odontol Latinoam ; 33(1): 50-55, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32621600

RESUMO

The purpose of this study was to evaluate aortic wall thickness after periodontal disease and/or obesity induction in a Wistar rat model.Sixty male Wistar rats were randomly divided into four groups: control (CT), periodontal disease (PD), obesity (OB), and obesity plus periodontal disease (OB+PD). Groups OB and OB+PD received cafeteria diet for 17 weeks. After they had acquired obesity (week 12), periodontal disease was induced by placing a silk ligature on the maxillary right second molar of groups PD and OB+PD. During the experimental period, body weight and Lee index were assessed. Mean alveolar bone loss (ABL) was evaluated, and aortas were prepared for histometric analysis of the aortic wall by ImageJ software. Body weight and Lee index increased in rats exposed to cafeteria diet. Mean ABL was higher in Groups PD and OB+PD than in control and OB (p<0.05). ABL was 18% higher in Group OB+PD than in Group PD, with statistically significant difference (p<0.001). Aortas were thicker in Groups OB and OB+PD than in control and PD groups, respectively (2.31mm ± 0.28 and 2.33 ± 0.29 vs. 2.18 ± 0.26 and 2.14 ± 0.27). Group OB differed significantly from the control group (p=0.036), and OB+PD and OB differed significantly from PD (p=0.004 and p= 0.001, respectively). Obesity alters aortic wall thickness in Wistar rats. However, the presence of periodontal disease did not affect the aortic wall thickness under the conditions of the present study.


Assuntos
Perda do Osso Alveolar/etiologia , Aterosclerose , Ligadura/efeitos adversos , Obesidade/complicações , Periodontite/complicações , Perda do Osso Alveolar/patologia , Animais , Modelos Animais de Doenças , Masculino , Periodontite/patologia , Ratos , Ratos Wistar
3.
J Contemp Dent Pract ; 21(5): 580-584, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32690844

RESUMO

AIM: To illustrate the treatment of a complex periodontal disease patient utilizing oral disease risk assessment. BACKGROUND: Periodontal disease is multifactorial in nature and requires addressing multiple factors in order to manage it successfully. Maintenance care is crucial for a favorable outcome, and risk assessment may help formulate the most suitable treatment plan and maintenance program. CASE DESCRIPTION: A 37-year-old man presented at the clinic for comprehensive periodontal treatment prior to replacing his missing teeth. He was healthy but smoked 5-10 cigarettes per day. Based on the clinical and radiographic examinations, the patient had generalized advanced periodontitis with multiple caries lesions, defective fillings, and missing teeth. A comprehensive treatment plan was put for the patient and a detailed assessment of his periodontal disease and caries risk was performed. The patient was assigned as being of high risk for periodontal disease progression and of low-medium caries risk. Three months following execution of the proposed nonsurgical and surgical treatment procedures, the patient demonstrated notable improvement compared to the baseline and was put under a strict maintenance program every 3 months. CONCLUSION: The presented case illustrates how oral disease risk assessment measures may be incorporated within comprehensive management of a periodontitis patient. Recommending this approach remains a personal preference and is yet to be substantiated by evidence. CLINICAL SIGNIFICANCE: Incorporating risk assessment measures in daily clinical practice may prevent the onset and/or progression of future disease, reducing unnecessary effort and expenses, and should be evaluated by concerned policymakers.


Assuntos
Cárie Dentária , Doenças Periodontais , Periodontite , Perda de Dente , Adulto , Humanos , Masculino , Medição de Risco
4.
Minerva Stomatol ; 69(3): 174-182, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32610725

RESUMO

BACKGROUND: As there are few studies in Europe describing characteristics of breath malodor for large groups of patients, this retrospective study was designed to analyse the etiology of halitosis among patients attending a breath malodor center in Northern Italy. METHODS: Clinical records of 547 consecutive patients were reviewed and data on self-perceived halitosis, organoleptic scores, volatile sulphur compound (VSC) levels, and oral health condition were extracted and analysed. RESULTS: The prevalence of intra-oral halitosis was 90.7%. In 21 patients no objective signs of breath malodor could be found. Periodontitis and gingivitis were the main cause of bad breath in 33.9% of subjects and in combination with tongue coating in 55.2%. Only eight subjects have tongue coating as the only cause of halitosis. Ear, nose and throat (ENT)/extra-oral causes were found in 5.2% of the patients. VSC concentrations were lower in the psychogenic halitosis group, whereas no statistically significant differences were detected when comparing intra-oral and extra-oral halitosis except for (CH3)2S. CONCLUSIONS: Psychogenic halitosis is a rare condition among subjects complaining of suffering from bad breath. The most prevalent cause of halitosis is intra-oral, in particular a combination of tongue coating and periodontal disease. Tongue coating is rarely the primary cause of oral malodor.


Assuntos
Halitose , Periodontite , Humanos , Itália , Estudos Retrospectivos , Língua
5.
Oral Health Prev Dent ; 18(2): 363-371, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32618459

RESUMO

PURPOSE: No information is available on the perception of the quality of care in patients treated for periodontitis. The purpose of this article was to assess how periodontitis-affected patients perceive the quality of periodontal treatment (PT) and to measure the factors which may influence it. MATERIALS AND METHODS: 306 subjects who completed PT were invited to participate. Questionnaires and visual analogic scales (VAS) evaluating perception of quality of care, symptoms, and oral health related quality of life (OHRQoL) were handed out. Oral and periodontal indicators were collected before and after treatment. The impact of different factors on perception of quality was assessed with a regression model. RESULTS: Quality evaluation was high yet unrelated for both patients and clinicians (p = 0.983). Quality was negatively influenced by the number of residual oral infections (p < 0.001), patient's age (p = 0.07) and presence of residual pain at completion of PT (p = 0.02). Professionalism, kindness of the staff and communication skills were the characteristics mostly appreciated. The OHRQoL was influenced by the number of residual teeth (p < 0.001), increasing age of patients (p = 0.08), number of residual infections (p < 0.01) and pain (p = 0.04). CONCLUSIONS: Patients' quality perception appeared to be influenced by clinical and emotional aspects. Oral care providers should be aware of the impact of non-clinical factors in patients' appreciation of quality of treatment.


Assuntos
Saúde Bucal , Periodontite , Assistência Odontológica , Humanos , Qualidade de Vida , Inquéritos e Questionários
6.
J Contemp Dent Pract ; 21(4): 372-376, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32584271

RESUMO

AIM: The review is to highlight the use of antibiotics in periodontal infections and prevent indiscriminate use of antibiotics. BACKGROUND: Periodontitis is the most common disease of the periodontal attachment apparatus, and its etiological factor can be related to the existence of virulent microorganisms in the dental plaque biofilm which harbors millions of microorganisms. In addition, the pathogenesis of this disease is greatly influenced by the host immune response that leads to the cyclic destruction and healing pattern. REVIEW RESULTS: Periodontitis is mostly treated through mechanical debridement using surgical and nonsurgical therapy. However, many times, this treatment does not render desired results due to poor patient compliance, altered immune response, or other host-related factors. This leads to the administration of antibiotics as an adjunct to mechanical debridement. Antibiotics are useful in eliminating periodontopathic microbes, but these agents should be cautiously used and prescribed only if indicated. CONCLUSION: Indiscriminate use of antibiotics can lead to unforeseen adverse effects as well as the development of resistant strains of microorganisms. CLINICAL SIGNIFICANCE: Hence, it is crucial for the dentists to know the indications, contraindications, undesirable effects, correct choice, and dosage of the antimicrobial agent before prescribing it to their patients thereby ensuring the success of periodontal therapy. Thus, the clinician should keep in mind that the antibiotics are merely adjuncts to mechanical therapy and not its replacement.


Assuntos
Placa Dentária/tratamento farmacológico , Infecções , Periodontite , Antibacterianos/uso terapêutico , Biofilmes , Humanos
7.
J Contemp Dent Pract ; 21(4): 431-437, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32584282

RESUMO

AIMS: Periodontitis is one of the most widespread diseases worldwide. Many efforts have been made to increase the efficacy of periodontitis therapy as much as possible. Recently, minimally invasive nonsurgical techniques (MINST) were introduced in the periodontal field as an alternative to minimally invasive surgical techniques (MIST). This clinical audit aims to evaluate the results of MINST in the initial phase of treatment for periodontitis. MATERIALS AND METHODS: One hundred seven patients with periodontitis who were treated with MINST between 2013 and 2017 and reevaluated after 2 months were included in this clinical audit. The primary outcome analyzed was the proportion of pocket closure. The secondary outcomes were tooth extraction before active periodontal therapy, full-mouth plaque score (FMPS) change, full-mouth bleeding score (FMBS) change, average probing pocket depth (PPD) reduction, and average clinical attachment level (CAL) gain between the baseline and reevaluation values. RESULTS: A total of 2,407 teeth were included in the analysis. At the patient level, the treatment resulted in a mean pocket closure rate of 71.6 ± 15.7% for sites with an initial PPD ≥5 mm. The treatment was statistically significantly (p < 0.001) more effective with respect to the primary outcome compared with expected values reported in a recent meta-analysis (57%). The subgroup analysis revealed statistically significant differences between single and multirooted teeth and between shallow (5-6 mm) and deep pockets (≥7 mm) at the baseline. CONCLUSION: Nonsurgical periodontal therapy with MINST achieved satisfactory results that were better than expected based on the scientific literature. Single-rooted and shallow pockets showed the best proportion of pocket closure at the reevaluation after treatment. CLINICAL SIGNIFICANCE: Minimally invasive nonsurgical techniques can be the treatment of choice when approaching periodontally diseased patients with nonsurgical periodontal therapy.


Assuntos
Periodontite , Auditoria Clínica , Raspagem Dentária , Humanos , Procedimentos Cirúrgicos Minimamente Invasivos , Perda da Inserção Periodontal , Índice Periodontal , Resultado do Tratamento
8.
Braz Oral Res ; 34: e058, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32578801

RESUMO

In view of the epidemiological relevance of periodontal disease and chronic noncommunicable diseases, the study aimed to evaluate the relationship between them through subclinical indicators of systemic risk in a population group with healthy habits, including alcohol and tobacco abstinence. A complete periodontal examination of six sites per tooth was performed in a sample of 420 participants from the Advento study (Sao Paulo), submitted to anthropometric and laboratory evaluation. Periodontitis was defined and classified based on the Community Periodontal Index score 3 (periodontal pocket = 4-5 mm) and score 4 (periodontal pocket ≥ 6 mm). The prevalence of mild/moderate and severe periodontitis was 20% and 8.2%, respectively. Both categories of periodontal disease had significantly higher levels of triglycerides, C-reactive protein, calcium score, and calcium percentile, whereas blood glucose after tolerance test was significantly higher among people with severe periodontitis and HDL-c levels were lower (p < 0.05). Young adults with severe periodontitis had significantly higher prevalence of obesity, pre-diabetes, hypertension, and metabolic syndrome. Besides these conditions, the older adults with severe periodontitis had significantly higher prevalence of dyslipidemia and subclinical atherosclerosis. The group with periodontitis had also a higher coronary heart disease risk based on the PROCAM score (p < 0.05). The results indicated associations of periodontitis with several systemic indicators for chronic noncommunicable diseases, and highlighted the need for multiprofessional measures in the whole care of patients.


Assuntos
Doenças não Transmissíveis/epidemiologia , Periodontite/complicações , Periodontite/epidemiologia , Adulto , Idoso , Brasil/epidemiologia , Doença Crônica , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Prevalência , Valores de Referência , Fatores de Risco , Índice de Gravidade de Doença , Fatores Socioeconômicos , Estatísticas não Paramétricas
9.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(3): 250-255, 2020 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-32573130

RESUMO

OBJECTIVE: This study aimed to compare the differences of B cells, plasma cells, and related cytokines expression in gingival tissues between periodontitis and periodontal healthy subjects. METHODS: Gingival tissues were collected from periodontal healthy subjects (periodontal healthy group, n=12) and periodontitis patients (periodontitis group, n=15). Hematoxylin-eosin (HE) staining was used for histopathological examination. Immunohistochemical staining (CD19, CD38, and CD138) was applied to detect the expression of B cells and plasma cells. B cell-activating factor (BAFF) and soluble receptor activator of nuclear factor-κB ligand (sRANKL) were detected by enzyme-linked immunosorbent assay. RESULTS: Extensive inflam-matory cell infiltration was found in the gingival tissues of the periodontitis group. The number of CD19(+), CD38(+), and CD138(+) cells of the periodontitis group was significantly higher than that of the periodontal healthy group (P<0.000 1). BAFF and sRANKL levels of the periodontitis group were higher than those of the periodontal healthy group (P<0.01, P<0.001, respectively). CONCLUSIONS: The expression of B cells, plasma cells, and their related BAFF and sRANKL cytokines were significantly higher in periodon-titis patients than those in the periodontal healthy subjects, sug-gesting that B cells and plasma cells may be involved in the development of periodontitis.


Assuntos
Periodontite , Plasmócitos , Linfócitos B , Citocinas , Líquido do Sulco Gengival , Voluntários Saudáveis , Humanos
11.
Braz Dent J ; 31(2): 122-126, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32556010

RESUMO

Although periodontitis is one of the commonest infectious inflammatory diseases in humans, the mechanisms involved with its immunopathology remain ill understood. Numerous molecules may induce inflammation and lead to bone resorption, secondary to activation of monocytes into osteoclasts. TACE (TNF-α converting enzyme) and DC-STAMP (dendritic cell-specific transmembrane protein) appear to play a role on bone resorption since TACE induces the release of sRANKL (soluble receptor activator of nuclear factor kappa-ß ligand) whereas DC-STAMP is a key factor in osteoclast induction. The present study evaluated the levels of TACE and DC-STAMP in patients with and without periodontitis. Twenty individuals were selected: 10 periodontally healthy participants undergoing gingivectomy for esthetic reasons and 10 diagnosed with periodontitis. Protein levels of such molecules in gingival tissue were established using Western blotting. Protein levels of both TACE and DC-STAMP were higher in the periodontitis group than in the control group (p<0.05; Student t-test). In conclusion, TACE and DC-STAMP protein levels are elevated in patients with periodontitis, favoring progression of bone resorption.


Assuntos
Proteína ADAM17 , Proteínas Adaptadoras de Transdução de Sinal , Reabsorção Óssea , Proteínas de Membrana , Periodontite , Proteína ADAM17/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Diferenciação Celular , Humanos , Proteínas de Membrana/metabolismo , Osteoclastos
12.
J Oral Sci ; 62(3): 250-255, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32418927

RESUMO

The aim of this study is to analyze the relationship between Hepatocyte Growth Factor (HGF) levels in oral rinses using water and clinical parameters of periodontitis; and furthermore, to evaluate the potential of a prototype HGF immunochromatographic paper test strip (HGF-TS) for screening of periodontitis, in comparison with a commercially-available occult blood (hemoglobin) test strip (Hb-TS). Clinical periodontal parameters were recorded, and oral rinses were collected, from 125 subjects. Then, the presence of HGF, and hemoglobin (Hb), in each sample was detected using a prototype HGF-TS and an Hb-TS. In addition, the concentrations of HGF and Hb were also determined in each sample is necessary HGF concentrations in oral rinses showed significant correlations with clinical parameters of periodontitis. The positive rate and read value on HGF-TS showed significantly high values in cases of severe periodontitis compared to healthy subjects. Hb-TS showed generally higher positive rates than HGF-TS; however, it showed false positive results in healthy subjects. The concentration of HGF in oral rinses showed close association with the severity of periodontitis, suggesting that the prototype HGF-TS has potential for use in the diagnosis of periodontitis, although further refinement of the test strip is required to increase the sensitivity.


Assuntos
Fator de Crescimento de Hepatócito , Periodontite , Humanos , Antissépticos Bucais , Água
13.
J Appl Oral Sci ; 28: e20190694, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32428060

RESUMO

Objective Obesity is a chronic disease that negatively affects an individual's general and oral health. The present study aimed to compare the clinical and microbiological effects of non-surgical periodontal therapy with the full mouth disinfection (FMD) protocol on obese and non-obese individuals at 9 months post-therapy. Methodology This clinical study was first submitted and approved by the Ethics Committee. Fifty-five obese patients and 39 non-obese patients with periodontitis were evaluated. The full-mouth periodontal clinical parameters, clinical attachment level (CAL), probing depth (PD), gingival index (GI), and plaque index (PI), were monitored at baseline, 3, 6, and 9 months after periodontal treatment with full mouth disinfection (FMD) protocol. The mean count of Tannerella forsythia , Porphyromonas gingivalis , Treponema Denticola , and Aggregatibacter actinomycetemcomitans was determined by quantitative polymerase chain reaction on subgingival biofilm samples. Demographic data were assessed by Chi-square test. For clinical and microbiological parameters, two-factor repeated-measures ANOVA was used. Results In both groups, periodontal therapy using the one-stage full-mouth disinfection protocol significantly improved CAL, PD, GI, and PI (p<0.05). Obese and non-obese patients equally responded to non-surgical periodontal therapy (p>0.05). Microbial count found no major differences (p>0.05) between obese and non-obese individuals who had undergone non-surgical periodontal therapy. Conclusions Obesity did not affect the clinical and microbiological outcomes of non-surgical periodontal therapy.


Assuntos
Obesidade/microbiologia , Periodontite/microbiologia , Periodontite/terapia , Adulto , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Análise de Variância , Antropometria , Índice de Placa Dentária , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Obesidade/fisiopatologia , Índice Periodontal , Porphyromonas gingivalis/isolamento & purificação , Estudos Prospectivos , Fatores de Risco , Estatísticas não Paramétricas , Tannerella forsythia/isolamento & purificação , Fatores de Tempo , Resultado do Tratamento , Treponema denticola/isolamento & purificação
14.
PLoS One ; 15(5): e0232912, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32392236

RESUMO

The study of oral disease progression, in relation to the accumulation of subgingival biofilm in gingivitis and periodontitis is limited, due to either the ability to monitor plaque in vitro. When compared, optical spectroscopic techniques offer advantages over traditional destructive or biofilm staining approaches, making it a suitable alternative for the analysis and continued development of three-dimensional structures. In this work, we have developed a confocal Raman spectroscopy analysis approach towards in vitro subgingival plaque models. The main objective of this study was to develop a method for differentiating multiple oral subgingival bacterial species in planktonic and biofilm conditions, using confocal Raman microscopy. Five common subgingival bacteria (Fusobacterium nucleatum, Streptococcus mutans, Veillonella dispar, Actinomyces naeslundii and Prevotella nigrescens) were used and differentiated using a 2-way orthogonal Partial Least Square with Discriminant Analysis (O2PLS-DA) for the collected spectral data. In addition to planktonic growth, mono-species biofilms cultured using the 'Zürich Model' were also analyzed. The developed method was successfully used to predict planktonic and mono-species biofilm species in a cross validation setup. The results show differences in the presence and absence of chemical bands within the Raman spectra. The O2PLS-DA model was able to successfully predict 100% of all tested planktonic samples and 90% of all mono-species biofilm samples. Using this approach we have shown that Confocal Raman microscopy can analyse and predict the identity of planktonic and mono-species biofilm species, thus enabling its potential as a technique to map oral multi-species biofilm models.


Assuntos
Bactérias/isolamento & purificação , Gengivite/microbiologia , Microscopia Óptica não Linear/métodos , Periodontite/microbiologia , Actinomyces , Técnicas Bacteriológicas/métodos , Biofilmes/crescimento & desenvolvimento , Meios de Cultura , Fusobacterium nucleatum , Gengiva/microbiologia , Viabilidade Microbiana , Microbiota , Microscopia Confocal/métodos , Plâncton , Prevotella intermedia , Streptococcus mutans , Veillonella
15.
Wiad Lek ; 73(5): 841-845, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32386355

RESUMO

OBJECTIVE: The aim: To study clinical efficiency of using the diode laser in the treatment of chronic catarrhal and hypertrophic gingivitis. PATIENTS AND METHODS: Materials and methods: Treatment of 32 patients with chronic catarrhal gingivitis and 30 patients with hypertrophic gingivitis by basic therapy according to the protocols of dental care was carried out. The patients of the main groups were additionally subjected to laser irradiation of the affected areas of the gums with a diode laser. RESULTS: Results: The use of laser therapy in the treatment of chronic catarrhal gingivitis contributed to the pain relief, reducing of bleeding and edema of the gums in 68.8% of patients on the 3rd day of observation. On the 7th day, 93.8% of the examined patients had no inflammation in periodontal tissues. During treatment of hypertrophic gingivitis, anti-inflammatory and decongestant effects, improvements in the aesthetic appearance of the gums were revealed in 33.3% patients on the 3rd day of the examination; absence of inflammation, gingival bleeding and gum hypertrophy was diagnosed in 53.3% on the 7th day and in 80.0% patients on 14th day of follow-up. In the control group, similar changes were revealed only in 68.8% patients with catarrhal gingivitis and in 46.7% patients with hypertrophic gingivitis after complete course of drug treatment in 14 days of observation. CONCLUSION: Conclusions: The use of a diode laser in patients with chronic gingivitis has provided a reduction in the term required for the complete elimination of the inflammatory process and suspended its further progression.


Assuntos
Gengivite , Terapia com Luz de Baixa Intensidade , Periodontite/terapia , Índice de Placa Dentária , Humanos , Lasers Semicondutores , Periodonto
16.
Rev Assoc Med Bras (1992) ; 66(2): 133-138, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32428146

RESUMO

OBJECTIVE: Periodontitis may stimulate infectious and immune response and cause the development of atherogenesis, coronary heart disease, and myocardial infarction. The aim of this study was to compare the plateletcrit (PCT) and mean platelet volume (MPV) levels derived from complete blood count (CBC) tests in patients suffering from stage 3 periodontitis with those of healthy individuals without periodontal disease. METHODS: The study included 57 patients (28 females and 29 males) with Stage 3 Periodontitis and 57 volunteering individuals (31 females and 26 males) who were periodontally healthy. The age of study participants ranged from 18 to 50 years. Their periodontal condition was investigated with probing depth (PD), clinical attachment level, bleeding on probing, and plaque index. Leukocyte (WBC) and erythrocyte count (RBC), hemoglobin (Hb) and hematocrit (HCT) levels, mean corpuscular volume (MCV) and red cell distribution width (RDW), thrombocyte count, mean platelet volume (MPV), plateletcrit (PCT ), and neutrophil and lymphocyte counts were evaluated based on the CBC test results of the study participants. RESULTS: PCT, WBC, Neutrophil, and MPV values were found to be significantly higher in the periodontitis group (p<0.05). There were no significant differences in RBC counts, Hb, HCT, MCV, RDW, and platelet and lymphocyte counts between the two study groups (p>0.05). CONCLUSIONS: PCT and MPV levels may be a more useful marker to determine an increased thrombotic state and inflammatory response in periodontal diseases.


Assuntos
Plaquetas/citologia , Volume Plaquetário Médio , Periodontite/sangue , Adolescente , Adulto , Contagem de Células Sanguíneas , Estudos de Casos e Controles , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Valores de Referência , Estatísticas não Paramétricas , Adulto Jovem
17.
Braz Oral Res ; 34: e038, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32374812

RESUMO

The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), two major cytokines associated to survival, differentiation and maturation of B cells in biopsies from gingival tissue with periodontitis. Gingival biopsies were obtained from subjects with periodontitis (n = 17), with periodontitis and DM (n = 19) as well as from periodontally and systemically healthy controls (n = 10). Gene expressions for APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were evaluated using qPCR. The expressions APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were all higher in both periodontitis groups when compared to the control group (p < 0.05). Furthermore, the expressions of BLyS, TRAP and RANKL were significantly higher in the subjects with periodontitis and DM when compared to those with periodontitis alone (p < 0.05). The mRNA levels of BLyS correlated positively with RANKL in the subjects with periodontitis and DM (p < 0.05). BLyS is overexpressed in periodontitis tissues of subjects with type 2 DM, suggesting a possible role of this cytokine on the pathogenesis DM-related periodontitis.


Assuntos
Fator Ativador de Células B/análise , Diabetes Mellitus Tipo 2/complicações , Periodontite/imunologia , Periodontite/patologia , Adulto , Idoso , Biomarcadores/análise , Biópsia , Estudos de Casos e Controles , Citocinas/análise , Citocinas/fisiologia , Diabetes Mellitus Tipo 2/imunologia , Feminino , Expressão Gênica , Gengiva/imunologia , Gengiva/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Osteogênese/imunologia , RNA Mensageiro/análise , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Estatísticas não Paramétricas , Membro 13 da Superfamília de Ligantes de Fatores de Necrose Tumoral/análise
18.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(1): 6-12, 2020 Jan 30.
Artigo em Chinês | MEDLINE | ID: mdl-32376562

RESUMO

OBJECTIVE: To investigate the expression of high mobility group box-1 protein (HMGB1) and its downstream products, receptor for advanced glycation end-products (RAGE) and tumor necrosis factor-α (TNF-α), in periodontal tissues of diabetic rats with periodontitis, and explore the association of HMGB1 with hepatic lipid metabolism. METHODS: Immunohistochemical staining was used to detect the expression of HMGB1, RAGE and TNF-α in the periodontal tissues in rat models of diabetes mellitus (DM), periodontitis (CP), and diabetic periodontitis (DM + CP). The serum levels of the indicators of lipid metabolism and biochemical indexes of liver damage were detected by spectroscopy. RESULTS: The expressions of HMGB1 and RAGE in the periodontal tissues were significantly higher in DM group than in the control group, but the expression of TNF-α showed no significant difference among the groups. In CP group, the expressions of HMGB1 and TNF-α were significantly higher than those in the control group, and the expression of RAGE was comparable with that in the control group but significantly lower than that in DM and DM+CP group. The expressions of HMGB1, RAGE and TNF-α were all significantly higher in DM+CP group than in the control group. Compared with the control rats, the rats in DM, CP, DM+CP group all showed abnormal hepatic lipid metabolism with significantly elevated serum ALT levels. CONCLUSIONS: HMGB1 and RAGE participate in the inflammation of the periodontal tissues in diabetic rats. Diabetes leads to elevated expression of HMGB1 in the periodontal tissues. Both periodontitis and hyperglycemia contribute to liver metabolic dysfunction. HMGB1- RAGE provides clues in the study of signaling pathways underlying the mutual susceptibility of diabetes and periodontitis.


Assuntos
Diabetes Mellitus Experimental , Proteína HMGB1/metabolismo , Metabolismo dos Lipídeos , Fígado/metabolismo , Periodontite/metabolismo , Animais , Ratos , Receptor para Produtos Finais de Glicação Avançada/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
19.
Periodontol 2000 ; 83(1): 66-89, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32385870

RESUMO

Cardiovascular diseases still account for the majority of deaths worldwide, although significant improvements in survival, after being affected by cardiovascular disease, have been achieved in the last decades. Periodontal diseases are also a common global burden. Several studies have shown a link between cardiovascular disease and periodontitis, although evidence is still lacking regarding the direct cause-effect relation. During the 2012 "Periodontitis and systemic diseases" workshop, the available evidence on the association between cardiovascular and periodontal diseases was discussed, covering biologic plausibility and clinical studies. The objective of the present narrative review was to update the previous reviews presented at the 2012 workshop, following similar methodological approaches, aiming to critically assess the available evidence. With regard to biologic plausibility, two aspects were reviewed: (a) for microbiologic mechanisms, assessing periodontal bacteria as a contributing factor to atherosclerosis based on seven "proofs," substantial evidence was found for Proofs 1 through 6, but not for Proof 7 (periodontal bacteria obtained from human atheromas can cause atherosclerosis in animal models), concluding that periodontal pathogens can contribute to atherosclerosis; (b) mechanistic studies, addressing five different inflammatory pathways that could explain the links between periodontitis and cardiovascular disease with the addition of some extra pathways , suggest an association between both entities, based on the presence of higher levels of these inflammatory markers in patients with periodontitis and cardiovascular disease, vs healthy controls, as well as on the evidence that periodontal treatment reduces serum levels of these mediators. When evidence from clinical studies was analyzed, two aspects were covered: (a) epidemiologic studies support the estimation that the incidence of atherosclerotic disease is higher in individuals with periodontitis than in individuals with no reported periodontitis, irrespective of many common risk factors, but with a substantial variability in the definitions used in reporting of exposure to periodontal diseases in different studies; (b) intervention trials have shown that periodontal therapy can reduce serum inflammatory mediators, improve the lipids profile, and induce positive changes in other cardiovascular disease surrogate measures, but no evidence is available to support that adequate periodontal therapy is able to reduce the risk for cardiovascular diseases, or the incidence of cardiovascular disease events in periodontitis patients.


Assuntos
Aterosclerose , Doenças Cardiovasculares , Doenças Periodontais , Periodontite , Animais , Humanos , Fatores de Risco
20.
Periodontol 2000 ; 83(1): 46-58, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32385872

RESUMO

The present narrative review examines the scientific evidence of the biological mechanisms that may link periodontitis and diabetes, as a source of comorbidity. Publications regarding periodontitis and diabetes, in human, animals, and in vitro were screened for their relevance. Periodontal microbiome studies indicate a possible association between altered glucose metabolism in prediabetes and diabetes and changes in the periodontal microbiome. Coinciding with this, hyperglycemia enhances expression of pathogen receptors, which enhance host response to the dysbiotic microbiome. Hyperglycemia also promotes pro-inflammatory response independently or via the advanced glycation end product/receptor for advanced glycation end product pathway. These processes excite cellular tissue destruction functions, which further enhance pro-inflammatory cytokines expression and alteration in the RANKL/osteoprotegerin ratio, promoting formation and activation of osteoclasts. The evidence supports the role of several pathogenic mechanisms in the path of true causal comorbidity between poorly controlled diabetes and periodontitis. However, further research is needed to better understand these mechanisms and to explore other mechanisms.


Assuntos
Diabetes Mellitus , Hiperglicemia , Doenças Periodontais , Periodontite , Animais , Humanos , Fatores de Risco
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