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1.
Virol J ; 17(1): 97, 2020 07 08.
Artigo em Inglês | MEDLINE | ID: mdl-32641169

RESUMO

BACKGROUND: Since the outbreak of Coronavirus Disease 2019 (COVID-19) in December 2019, many studies have reported the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the conjunctival sac of patients infected with this virus, with several patients displaying symptoms of viral conjunctivitis. However, to our best knowledge, there is no in-depth report on the course of patients with COVID-19 complicated by relapsing viral conjunctivitis or keratoconjunctivitis. CASE PRESENTATION: A 53-year-old man confirmed with COVID-19 developed symptoms of viral conjunctivitis in the left eye approximately 10 days after the onset of COVID-19. The results of a nucleic acid test were positive for SARS-CoV-2 in the conjunctival sac of the left eye. The symptoms were relieved 6 days after treatment. However, the patient was subsequently diagnosed with viral keratoconjunctivitis in both eyes 5 days after the symptoms in the left eye were satisfactorily relieved. The disease progressed rapidly, with spot staining observed at the periphery of the corneal epithelium. Although SARS-CoV-2 could not be detected in conjunctival secretions, the levels of inflammatory factors, such as interleukin-6, were increased in both eyes. Both eyes were treated with glucocorticoids, and symptoms were controlled within 5 days. There was no recurrence. CONCLUSIONS: In this case report, the pathogenesis, clinical manifestations, treatment, and outcome of a case with COVID-19 complicated by relapsing viral keratoconjunctivitis is described, and the involvement of topical cytokine surge in the pathogenesis of COVID-19 as it relates to viral keratoconjunctivitis is reported.


Assuntos
Betacoronavirus/patogenicidade , Conjuntivite Viral/complicações , Infecções por Coronavirus/complicações , Ceratoconjuntivite/complicações , Pneumonia Viral/complicações , Betacoronavirus/isolamento & purificação , Conjuntivite Viral/tratamento farmacológico , Conjuntivite Viral/patologia , Conjuntivite Viral/virologia , Infecções por Coronavirus/tratamento farmacológico , Infecções por Coronavirus/patologia , Infecções por Coronavirus/virologia , Síndrome da Liberação de Citocina , Glucocorticoides/uso terapêutico , Humanos , Ceratoconjuntivite/tratamento farmacológico , Ceratoconjuntivite/patologia , Ceratoconjuntivite/virologia , Aparelho Lacrimal/virologia , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/tratamento farmacológico , Pneumonia Viral/patologia , Pneumonia Viral/virologia , Recidiva , Resultado do Tratamento
2.
In Vivo ; 34(3 Suppl): 1619-1628, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32503820

RESUMO

The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in China in the city of Wuhan in December of 2019 and since then more than 5,000,000 people have been infected, with approximately 338,000 deaths worldwide. The virus causes the coronavirus disease 2019 (COVID-19), which is characterized by fever, myalgia and cough, with severe acute respiratory syndrome being the most fearsome complication. Nevertheless, the vast majority of cases present mild symptoms or none. Central nervous system and cardiovascular manifestations have been reported. The range of ocular manifestations, either as a result of the infection or as a result of the treatment, has not yet been discussed. In this study, a systematic review of current literature relevant to COVID-19 was performed with focus on modes of transmission, ocular manifestations related to infection and medications, as well as the control of infection in ophthalmic practice.


Assuntos
Infecções por Coronavirus/complicações , Oftalmopatias/etiologia , Pneumonia Viral/complicações , Doenças do Nervo Abducente/etiologia , Antivirais/efeitos adversos , Antivirais/uso terapêutico , Betacoronavirus/fisiologia , Biomarcadores , China , Doenças da Túnica Conjuntiva/sangue , Doenças da Túnica Conjuntiva/etiologia , Lentes de Contato/efeitos adversos , Infecções por Coronavirus/tratamento farmacológico , Infecções por Coronavirus/terapia , Infecções por Coronavirus/transmissão , Contaminação de Equipamentos , Oftalmopatias/prevenção & controle , Humanos , Hiperemia/sangue , Hiperemia/etiologia , Imunização Passiva/efeitos adversos , Transmissão de Doença Infecciosa do Paciente para o Profissional/prevenção & controle , Ceratoconjuntivite/etiologia , Doenças do Aparelho Lacrimal/sangue , Doenças do Aparelho Lacrimal/etiologia , Contagem de Leucócitos , Pandemias , Pneumonia Viral/tratamento farmacológico , Pneumonia Viral/transmissão , Doenças Retinianas/induzido quimicamente , Estudos Retrospectivos
4.
Klin Monbl Augenheilkd ; 237(5): 675-680, 2020 May.
Artigo em Inglês, Alemão | MEDLINE | ID: mdl-32375197

RESUMO

PURPOSE: To perform a systematic analysis of articles on the ophthalmological implications of the global COVID-19 pandemic. METHODS: PubMed.gov was searched for relevant articles using the keywords "COVID-19", "coronavirus", and "SARS-CoV-2" in conjunction with "ophthalmology" and "eye". Moreover, official recommendations of ophthalmological societies were systematically reviewed, with a focus on the American Academy of Ophthalmology (AAO) and the Royal College of Ophthalmologists (RCOphth). RESULTS: As of April 16, 2020, in total, 21 peer-reviewed articles on the ophthalmological aspects of COVID-19 were identified. Of these, 12 (57.1%) were from Asia, 6 (28.6%) from the United States of America, and 3 (14.3%) from Europe. There were 5 (23.8%) original studies, 10 (47.6%) letters, 3 (14.2%) case reports, and 3 (14.2%) reviews. These articles could be classified into the topics "Modes and prevention of (ocular) transmission", "Ophthalmological manifestations of COVID-19", "Clinical guidance concerning ophthalmological practice during the COVID-19 pandemic", and "Practical recommendations for clinical infrastructure". Practical recommendations could be extracted from official statements of the AAO and the RCOphth. CONCLUSION: Within a short period, a growing body of articles has started to elucidate the ophthalmological implications of COVID-19. As the eye can represent a route of infection (actively via tears and passively via the nasoacrimal duct), ophthalmological care has to undergo substantial modifications during this pandemic. In the eye, COVID-19 can manifest as keratoconjunctivitis.


Assuntos
Infecções por Coronavirus , Ceratoconjuntivite , Ducto Nasolacrimal/virologia , Oftalmologia , Pandemias , Pneumonia Viral , Vírus da SARS , Lágrimas/virologia , Betacoronavirus/patogenicidade , Infecções por Coronavirus/complicações , Infecções por Coronavirus/transmissão , Humanos , Ceratoconjuntivite/virologia , Pneumonia Viral/complicações , Pneumonia Viral/transmissão
5.
BMC Ophthalmol ; 19(1): 262, 2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31856790

RESUMO

BACKGROUND: Epidemic keratoconjunctivitis (EKC) is one of the most common eye infections worldwide. The analysis of clinical manifestations in different age groups help better know the disease. This study aims to provide more detailed analysis of 272 cases of EKC in 8 years, describe the differences of the clinical features among different age groups, and establish new clinical grading criteria. METHODS: 272 individuals were reviewed (2011-2019) in Beijing China. All the patients were classified into 3 grades according to the new grading criteria. The typical clinical signs of EKC and the photographs of the multiple subepithelial corneal infiltrates (MSI) were collected and analyzed. The number of 3 grades among and within different age groups were compared. The incidence of the typical signs among and within different age groups were compared. The proportion of each region of the cornea involved by MSI were compared. RESULTS: No significant differences were detected among the 4 groups in terms of the number of mild, moderate and severe cases, no matter in all-patients analysis (P = 0.271) nor in acute-phase-patients analysis (P = 0.203). The proportion of the severe cases was the highest among all patients (P = 0.000). Among the incidence of the typical signs, corneal involvement was the most common accounting for 69.8% (P<0.05). The probability of central region involvement was significantly higher than that of pericentral region involvement (P = 0.015) and peripheral region involvement (P = 0.000). CONCLUSIONS: Appropriate attention should be paid on EKC, because of the considerable proportion of severe cases, the high incidence of corneal lesion, and the high incidence of central region involvement of MSI.


Assuntos
Infecções por Adenovirus Humanos/diagnóstico , Conjuntivite Viral/diagnóstico , Infecções Oculares Virais/diagnóstico , Ceratoconjuntivite/diagnóstico , Infecções por Adenovirus Humanos/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Pequim/epidemiologia , Criança , Pré-Escolar , Conjuntivite Viral/epidemiologia , Infecções Oculares Virais/epidemiologia , Feminino , Humanos , Incidência , Ceratoconjuntivite/epidemiologia , Masculino , Microscopia Confocal , Pessoa de Meia-Idade , Estudos Retrospectivos , Estações do Ano , Adulto Jovem
6.
Vestn Oftalmol ; 135(5. Vyp. 2): 192-198, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31691659

RESUMO

Ophthalmologic manifestation of Sjogren's disease (SD) and rheumatoid arthritis (RA) is dry keratoconjunctivitis (dry eye disease; DED). PURPOSE: To study the relationship of polymorphic markers rs7947461 (C/T), rs915956 (C/T), rs4144331 (C/A) of the TRIM21 gene with the severity of DED in patients with RA and SD. MATERIAL AND METHODS: The study included 70 patients with RA (n=27) and SD (n=43). The control group consisted of volunteers without a history of RA or SD (n=35). Alleles of the polymorphic marker C660T rs7947461 of the TRIM21 gene were identified using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method; alleles of the polymorphic marker rs915956 (C/T) and rs4144331 (C/A) of the TRIM21 gene were identified by analyzing DNA melting curves. RESULTS: An association was found between the predisposing genotype (TT) of rs7947461 polymorphic marker and the risk of developing severe DED. The AA genotype of rs4144331 polymorphic marker was found only in severe DED (c2=7.74; OR=17.46, CI95%=1.96-318.38, p=0.02). CONCLUSION: An association was established between rs7947461 (rs660) and rs4144331 and the risk of developing severe DED.


Assuntos
Artrite Reumatoide , Ceratoconjuntivite , Ribonucleoproteínas/genética , Síndrome de Sjogren , Alelos , Artrite Reumatoide/genética , Predisposição Genética para Doença , Genótipo , Humanos , Ceratoconjuntivite/genética , Polimorfismo Genético , Síndrome de Sjogren/genética
7.
Vestn Oftalmol ; 135(5. Vyp. 2): 254-259, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31691669

RESUMO

The article reviews literature on relationships between polymorphic variants of the genes THBS1, GTF2I, MUC1, TRIM21, STAT4, PTPN22 with clinical features of dry keratoconjunctivitis in rheumatoid arthritis and Sjogren's syndrome. The development and implementation of a method for analyzing polymorphic gene variants used to diagnose dry keratoconjunctivitis in rheumatoid arthritis and Sjogren's syndrome will allow assessment of the possibility of developing dry keratoconjunctivitis and/or its progression in patients with autoimmune diseases or in people at risk. Determination of clinical and morphological regularities of dry keratoconjunctivitis in accordance with the revealed molecular and genetic changes will contribute to better understanding of the etiology and pathogenesis of ophthalmological manifestations of autoimmune diseases, and will also help improve the diagnostics and prognosis of dry keratoconjunctivitis.


Assuntos
Artrite Reumatoide , Ceratoconjuntivite , Síndrome de Sjogren , Biomarcadores , Humanos , Prognóstico , Proteína Tirosina Fosfatase não Receptora Tipo 22
8.
Tunis Med ; 97(5): 639-643, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31729734

RESUMO

INTRODUCTION: The adenoviral keratoconjunctivitis infiltrates may be a source of significant visual impairment justifying the use of various therapeutic means. AIM: Evaluate the efficiency and safety of use of cyclosporine A 0,5% eye drop in the treatment of subepithelial infiltrates. METHODS: It was a prospective study of 37 eyes of 22 patients with adenoviral keratoconjunctivitis with subepithelial infiltrates treated with cyclosporine A 0,5% eye drop. Cyclosporine A 0,5% was prepared from the injectable form of cyclosporine (Sandimmun®) and artificial tears. The cyclosporine A 0,5% was first administered at 4 drops per day for 15 days, then at a rate of 2 drops per day for a variable period ranging from 15 days to 6 months. The use of this molecule has been motivated by the presence of a persistent dazzlement, by visual acuity under 6/10 or an astigmatism superior to 1 diopter. RESULTS: At the end of follow, dazzlement disappeared in all patients; the final average visual acuity was 8/10 and corneal astigmatism average was of 0.75 diopter. The slit lamp examination showed a marked decrease in the number and density of subepithelial infiltrates from the 15th day. A 29-year-old patient, however, presented some intercostal vesicles due to zonal recrudescence but with spontaneous and quick resolve in the same time of taking topical cyclosporine. No local complications were observed in our patients. The average follow-up was 13 months. CONCLUSION: Topical cyclosporine A is an effective and well-tolerated alternative to corticosteroids in the subepithelial infiltrates occurring as sequelae of adenoviral keratoconjunctivitis.


Assuntos
Infecções por Adenoviridae/tratamento farmacológico , Ciclosporina/administração & dosagem , Ceratoconjuntivite/tratamento farmacológico , Ceratoconjuntivite/virologia , Adulto , Ciclosporina/efeitos adversos , Feminino , Humanos , Masculino , Soluções Oftálmicas , Estudos Prospectivos , Resultado do Tratamento
9.
Diagn Microbiol Infect Dis ; 95(4): 114885, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31607514

RESUMO

Adenoviral epidemic keratoconjunctivitis (EKC) is a major cause of ocular morbidity worldwide and specific antiviral therapies are not available. EKC is primarily caused by Human adenovirus D (HAdV-D) types 8, 37, 53, 54, 56 and 64. Considering the genomic variation in HAdV-D, we hypothesized that clinical signs could be differentiated by virus type. The hypothesis was retrospectively tested with clinical signs recorded from 250 patients with ocular infections visiting an ophthalmological clinic in southern Japan between 2011 and 2014. The results showed that conjunctival opacity, corneal epithelial disorders and pre-auricular lymphadenopathy, were more frequently associated with EKC than other ocular infections. Furthermore, HAdV types 8, 37 and 54, caused corneal complications and longer infections significantly more frequently than infections by types 53 and 56 (P < 0.05). Our descriptive results supported that symptoms severity vary with the infecting type, however, further research is needed to improve diagnosis of EKC.


Assuntos
Infecções por Adenovirus Humanos/epidemiologia , Infecções por Adenovirus Humanos/patologia , Adenovírus Humanos/fisiologia , Ceratoconjuntivite/epidemiologia , Ceratoconjuntivite/patologia , Células A549 , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos/classificação , Adenovírus Humanos/genética , Adenovírus Humanos/isolamento & purificação , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Efeito Citopatogênico Viral , Humanos , Lactente , Japão/epidemiologia , Ceratoconjuntivite/virologia , Pessoa de Meia-Idade , Tipagem Molecular , Estudos Retrospectivos , Adulto Jovem
10.
J Vet Intern Med ; 33(6): 2786-2793, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31605550

RESUMO

BACKGROUND: Infectious bovine keratoconjunctivitis (IBK) in beef cattle has major welfare and production implications. Effective vaccination against IBK would also reduce antibiotic use in beef production. OBJECTIVE/HYPOTHESIS: To evaluate the efficacy of a conditionally licensed commercial IBK vaccine containing Moraxella bovoculi bacterin. Primary working hypothesis was that animals vaccinated with 2 doses of the commercial M. bovoculi vaccine would have a lower risk of disease. ANIMALS: Spring born calves at a university cow-calf herd. After excluding animals with ocular lesions, calves eligible for prevention assessment in 2017 and 2018 were 163 (81 vaccinated, 82 unvaccinated) and 207 (105 vaccinated, 102 unvaccinated). One hundred sixty two and two hundred and six calves completed the follow-up period in 2017 and 2018, respectively. METHODS: A randomized controlled trial. The trial design was a 2-arm parallel trial with a 1:1 allocation ratio. RESULTS: In both years, calves receiving the vaccine had more IBK. This effect was small. The pooled risk ratio was 1.30 (95% confidence interval 0.84-2.01). The pooled unadjusted difference in mean weight (kg) at weaning was -0.88 (95% confidence interval-7.2-5.43). CONCLUSIONS AND CLINICAL IMPORTANCE: We were unable to document that the M. bovoculi bacterin vaccine had a protective effect for the incidence of IBK in our single herd in a 2-year study.


Assuntos
Vacinas Bacterianas/imunologia , Doenças dos Bovinos/prevenção & controle , Ceratoconjuntivite/veterinária , Moraxella/imunologia , Infecções por Moraxellaceae/veterinária , Animais , Bovinos , Humanos , Ceratoconjuntivite/microbiologia , Ceratoconjuntivite/prevenção & controle , Infecções por Moraxellaceae/microbiologia , Infecções por Moraxellaceae/prevenção & controle , Fatores de Risco
11.
Sci Adv ; 5(9): eaax3567, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31517055

RESUMO

Adenoviruses are clinically important agents. They cause respiratory distress, gastroenteritis, and epidemic keratoconjunctivitis. As non-enveloped, double-stranded DNA viruses, they are easily manipulated, making them popular vectors for therapeutic applications, including vaccines. Species D adenovirus type 26 (HAdV-D26) is both a cause of EKC and other diseases and a promising vaccine vector. HAdV-D26-derived vaccines are under investigation as protective platforms against HIV, Zika, and respiratory syncytial virus infections and are in phase 3 clinical trials for Ebola. We recently demonstrated that HAdV-D26 does not use CD46 or Desmoglein-2 as entry receptors, while the putative interaction with coxsackie and adenovirus receptor is low affinity and unlikely to represent the primary cell receptor. Here, we establish sialic acid as a primary entry receptor used by HAdV-D26. We demonstrate that removal of cell surface sialic acid inhibits HAdV-D26 infection, and provide a high-resolution crystal structure of HAdV-D26 fiber-knob in complex with sialic acid.


Assuntos
Adenovírus Humanos/química , Ácido N-Acetilneuramínico/química , Receptores Virais/química , Proteínas Virais/química , Vacinas contra Adenovirus/metabolismo , Adenovírus Humanos/metabolismo , Cristalografia por Raios X , Células HEK293 , Humanos , Ceratoconjuntivite/epidemiologia , Ceratoconjuntivite/metabolismo , Ceratoconjuntivite/patologia , Ácido N-Acetilneuramínico/metabolismo , Receptores Virais/metabolismo , Proteínas Virais/metabolismo
12.
Immunobiology ; 224(6): 728-733, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31526676

RESUMO

PURPOSE: Autoimmune polyendocrine type 1 (APS-1) is a complex inherited autosomal recessive disorder. Classically, it appears within the first decade of life followed by adrenocortical insufficiency, mucocutaneous candidiasis, Addison's disease, and hypoparathyroidism. The clinical phenotype of APS-1 varies depending upon mutations in the autoimmune regulator gene (AIRE) on chromosome 21q22.3. METHODS: In this study, we performed Sanger sequencing ofAIRE in Iranian patients to identify different variants and probable new mutations corresponding to a clinical diagnosis of APS-1. RESULTS: After analyzing 14AIRE exons, we detected a novel insertion mutation in exon 2 in a patient who presented with severe APS-1, Lys50AsnfsX168. Furthermore, the known mutations in AIRE, including Arg139X, Arg257X, and Leu323SerfsX51, were detected in enrolled patients. DISCUSSION: According to our results, sequencing analysis ofAIRE provides a useful screening method to diagnose patients with incomplete or unusual clinical presentations of APS-1.


Assuntos
Poliendocrinopatias Autoimunes/genética , Fatores de Transcrição/genética , Adolescente , Insuficiência Adrenal/genética , Adulto , Alopecia/genética , Candidíase Mucocutânea Crônica/genética , Criança , Hipoplasia do Esmalte Dentário/genética , Éxons , Feminino , Humanos , Hipoparatireoidismo/genética , Irã (Geográfico) , Ceratoconjuntivite/genética , Síndromes de Malabsorção/genética , Masculino , Mutação , Doenças da Unha/genética , Adulto Jovem
14.
Aust Vet J ; 97(10): 401-403, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31286490

RESUMO

Infectious Ovine Keratoconjunctivitis (IOK) is a contagious ocular disease of sheep. A range of organisms have been observed as the aetiological agents of IOK. In this study, the presence of chlamydial pathogens (C. pecorum, C. abortus, C. psittaci) in conjunctival swabs was tested for. The swabs were collected from sheep with varying grades of IOK in an Australian pre-export feedlot. The sheep had been rejected from a shipment because of the eye disease. The relative contribution of chlamydial pathogens to IOK and the rejection of animals was evaluated. In total, 149 conjunctival swabs were taken from rejected sheep (IOK Grades 1 to 6; n = 126) as well as those with healthy eyes (Grade 0; n = 23). Screening for chlamydial pathogens was done using species-specific qPCR assays. Chlamydial DNA was detected in 35.6% (53/149) of conjunctival samples. C. pecorum was the most predominant species with an overall prevalence of 28.9% (43/149). C. psittaci prevalence was 6.7% (10/149). Both organisms were detected in healthy as well as IOK-affected eyes. All swabs tested negative for C. abortus. The results from this study demonstrate that Chlamydia spp can be readily detected in sheep presenting with IOK. The zoonotic C. abortus was not detected in any of the samples in this study, providing further evidence to the suggestion that this pathogen remains absent from Australia. Although the exact contribution of Chlamydia spp in the IOK pathogenesis is unclear, such studies are anticipated to be of benefit to Australian domestic and live export production systems.


Assuntos
Infecções por Chlamydiaceae/veterinária , Chlamydiaceae/isolamento & purificação , Olho/microbiologia , Ceratoconjuntivite/veterinária , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/microbiologia , Animais , Austrália/epidemiologia , Infecções por Chlamydiaceae/epidemiologia , Ceratoconjuntivite/epidemiologia , Ceratoconjuntivite/microbiologia , Índice de Gravidade de Doença , Ovinos
15.
Cornea ; 38(11): 1418-1423, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31259862

RESUMO

PURPOSE: To explore the tolerability of automated conjunctival hyperemia quantification in children with blepharokeratoconjunctivitis (BKC) and its agreement with clinical activity grading and to explore the Children's Health Utility 9D (CHU9D) as a measure of health-related quality of life in children with BKC. METHODS: We enrolled 63 children, 31 with BKC and 32 without ocular surface inflammation, with a median [interquartile range (IQR)] age of 10.6 (7.2-13.9) years for BKC and 11.4 (9.5-13.8) years for healthy volunteers. Two masked observers graded the ocular surface images. The children indicated discomfort during imaging on a 5-point Likert scale. Using nonparametric tests, we explored the interobserver agreement and the agreement of automated redness index (RI) measurements of limbal and bulbar conjunctival hyperemia with clinician assessment. The children also completed the 9-item CHU9D. RESULTS: The children tolerated imaging well: median (IQR) Likert value of 0 ("comfortable") (0-0) in healthy volunteers and 1 ("a little bit uncomfortable") (0-2) in mild/moderate BKC. In children with BKC, the median (IQR) bulbar RI was 1.3 (0.8-1.6) and the median limbal RI was 0.7 (0.3-1.1). In healthy volunteers, the median bulbar RI was 0.8 (0.55-1.1; P = 0.162) and the median limbal RI was 0.3 (0.2-0.4; P = 0.02). The agreement between RI and clinical grading was high. There was no significant difference between the mean CHU9D utility score between the 2 groups [0.89 (SD 0.08) vs. 0.92 (SD 0.07); P = 0.15]. CONCLUSIONS: Automated conjunctival hyperemia quantification is feasible in children with ocular surface inflammation and may prove useful for long-term monitoring and as an objective outcome measure in clinical trials.


Assuntos
Blefarite/diagnóstico , Técnicas de Diagnóstico Oftalmológico , Nível de Saúde , Ceratoconjuntivite/diagnóstico , Qualidade de Vida , Adolescente , Blefarite/complicações , Blefarite/psicologia , Criança , Pré-Escolar , Túnica Conjuntiva/patologia , Córnea/patologia , Pálpebras/patologia , Estudos de Viabilidade , Feminino , Seguimentos , Humanos , Ceratoconjuntivite/complicações , Ceratoconjuntivite/psicologia , Masculino , Estudos Prospectivos
16.
Cornea ; 38(8): 943-947, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31276457

RESUMO

PURPOSE: To describe the findings of anterior segment optical coherence tomography (AS-OCT) in patients with microsporidial keratoconjunctivitis. METHODS: The observational study included 13 eyes from 13 patients. Slit-lamp photography and AS-OCT were performed using the Swept source OCT before corneal scraping. All cases were positive for Gram-chromotrope (modified trichrome) staining for Microsporidia spp. RESULTS: Three significant AS-OCT findings were observed. First, hyperreflective dots were limited to the epithelial layers of the cornea, and second, there were no extensions into the stromal layer in all cases. Last, hyperreflective dots slightly raised above the epithelial surface were observed in most cases (12/13%, 92.3%). CONCLUSIONS: AS-OCT represents an alternative, noninvasive tool to diagnose microsporidial keratoconjunctivitis, especially if corneal scraping is not possible.


Assuntos
Segmento Anterior do Olho/diagnóstico por imagem , Infecções Oculares Fúngicas/diagnóstico por imagem , Ceratoconjuntivite/diagnóstico por imagem , Microsporídios/isolamento & purificação , Microsporidiose/diagnóstico por imagem , Tomografia de Coerência Óptica/métodos , Adulto , Antibacterianos/uso terapêutico , Desbridamento , Infecções Oculares Fúngicas/tratamento farmacológico , Infecções Oculares Fúngicas/microbiologia , Feminino , Humanos , Ceratoconjuntivite/tratamento farmacológico , Ceratoconjuntivite/microbiologia , Masculino , Microsporidiose/tratamento farmacológico , Microsporidiose/microbiologia , Pessoa de Meia-Idade , Moxifloxacina/uso terapêutico , Microscopia com Lâmpada de Fenda , Acuidade Visual
17.
Vet Ophthalmol ; 22(6): 928-932, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31339654

RESUMO

A 6-year-old male leopard gecko (Eublepharis macularius) was presented with a 2-year history of recurrent dysecdysis involving the ocular surface of both eyes. Ophthalmic examination revealed ocular surface desiccation and multifocal superficial ulcerative keratitis with patchy remnants of retained shed. Other abnormalities included stomatitis and mandibular and maxillary osteomyelitis. Topical and systemic antibiotic therapy, oral vitamin A, and improved husbandry conditions resolved the stomatitis and osteomyelitis but did not improve the ocular surface. Corneal cytology collected with a cytobrush revealed branching hyphae and budding yeast consistent with fungal keratitis. Fungal culture grew Acremonium sp. and Trichosporon sp. The addition of topical antifungal therapy improved the ocular surface health, but the patient was euthanized 7 weeks after initial presentation for persistent vomiting and dyspnea. Necropsy was declined. This case describes the first case of fungal keratitis caused by Acremonium sp. and Trichosporon sp. in a reptile.


Assuntos
Acremonium/isolamento & purificação , Infecções Oculares Fúngicas/veterinária , Ceratoconjuntivite/veterinária , Lagartos/microbiologia , Trichosporon/isolamento & purificação , Animais , Anti-Infecciosos/uso terapêutico , Infecções Oculares Fúngicas/tratamento farmacológico , Infecções Oculares Fúngicas/microbiologia , Evolução Fatal , Ceratoconjuntivite/microbiologia , Masculino
18.
BMC Infect Dis ; 19(1): 624, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31307413

RESUMO

BACKGROUND: Two outbreaks of epidemic keratoconjunctivitis (EKC) occurred successively with an interval of 5 days in two primary boarding schools in Weixi Lisu Autonomous County, Diqing, and Tibetan Autonomous Prefecture, Yunnan. The aims of this study were to determine the intensity and characteristics of the outbreaks, as well as the clinical manifestations in the patients, the risk factors for infection and the pathogen responsible for the two outbreaks. METHODS: An outbreak investigation was conducted in two primary schools, and a case-control study including patients from the Weixi County Ethnic Primary School was performed. Relevant specimens were collected according to the case definition, and next-generation sequencing was employed to identify the pathogen. An epidemiological investigation method was used to analyse the related epidemiological characteristics, such as risk factors. The phylogenetic tree was constructed by MEGA 7.0. RESULTS: A total of 331 acute conjunctivitis cases, including probable cases of EKC, were reported in the two schools, and the attack rates were 30.59% (171/559, 95%CI: 26.76-34.42) and 20.41% (160/784, 95%CI: 17.58-23.24), respectively. Cases occurred in all grades and classes in both schools, and only one staff member in each school presented illness. The epidemics lasted for 54 days and 45 days, respectively. The patients had typical manifestations of EKC, such as acute onset, follicular hyperplasia, pseudomembrane formation, preauricular lymphadenopathy, corneal involvement and blurred vision, and a relatively long disease course (average 9.40 days, longest 23 days and shortest 7 days). The risk factor for infection was close contact with a patient or personal items contaminated by a patient. The pathogen responsible for the outbreaks was HAdV-8. The virus was highly similar to the 2016 HAdV-8 strain from Tibet, China. CONCLUSIONS: This study strongly suggests that HAdV-8 could lead to serious consequences. This is the second report of a HAdV-8-associated EKC outbreak in mainland China. Tibetan HAdV-8 might be circulating in southwest China; therefore, it is necessary to monitor the pathogens causing acute conjunctivitis in this area.


Assuntos
Infecções por Adenovirus Humanos/diagnóstico , Adenovírus Humanos/isolamento & purificação , Ceratoconjuntivite/diagnóstico , Infecções por Adenovirus Humanos/epidemiologia , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos/classificação , Adenovírus Humanos/genética , Adolescente , Adulto , Estudos de Casos e Controles , Criança , China/epidemiologia , DNA Viral/isolamento & purificação , DNA Viral/metabolismo , Surtos de Doenças , Feminino , Humanos , Ceratoconjuntivite/epidemiologia , Ceratoconjuntivite/virologia , Masculino , Filogenia , Fatores de Risco , Instituições Acadêmicas , Análise de Sequência de DNA , Adulto Jovem
20.
Jpn J Ophthalmol ; 63(5): 410-416, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31243592

RESUMO

PURPOSE: To elucidate the recent trends in prevalence and characteristics of ocular complications of atopic dermatitis (AD). STUDY DESIGN: Cross-sectional observational study. METHODS: Among AD patients who visited our department between 2012 and 2015, 70 patients (140 eyes; recent AD group) who gave informed consent to participate in the study were analyzed. Following a medical interview, ophthalmological examinations were conducted for ocular complications related to AD. The data were compared to those of 280 AD patients (560 eyes) analyzed in a similar study conducted at our department in 1991-1993 (previous AD group). RESULTS: Blepharitis was found in 58 eyes (41%) in the recent AD group, and the frequency was significantly lower compared to the previous AD group (294 eyes, 53%) (p < 0.05). Tears in retina or pars plana ciliaris occurred in 22 eyes (4%) in the previous AD group, compared with none in the recent AD group (p < 0.01). Retinal detachment was observed in 12 eyes (2%) in the previous AD group, and none in the recent AD group (p < 0.01). Atopic keratoconjunctivitis (AKC) increased significantly in the recent AD group compared to the previous AD group (74.3% vs. 39.5%) (p < 0.001). Patients with a habit of slapping around the eye decreased significantly from 32.5% in the previous AD group to 12.1% in the recent AD group (p < 0.001). CONCLUSIONS: Ocular complications in AD patients show a trend of decrease in recent years, which presumably is attributed to educational activities to increase patient awareness and advances in therapeutic strategy.


Assuntos
Dermatite Atópica/complicações , Ceratoconjuntivite/etiologia , Descolamento Retiniano/etiologia , Adulto , Distribuição por Idade , Estudos Transversais , Feminino , Humanos , Incidência , Japão/epidemiologia , Ceratoconjuntivite/epidemiologia , Masculino , Descolamento Retiniano/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Distribuição por Sexo
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