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1.
Zhonghua Gan Zang Bing Za Zhi ; 28(6): 532-535, 2020 Jun 20.
Artigo em Chinês | MEDLINE | ID: mdl-32660187

RESUMO

Liver failure is a rapidly progressive condition with a high mortality rate. Artificial liver treatment is one of the most important treatments for liver failure. In order to ensure the smooth functioning of in vitro circulation device, anticoagulants such as heparin or low-molecular-weight heparin are often used in clinical practice. However, it induces hemorrhage, thrombocytopenia and other adverse reactions, thereby threatening the life safety of liver failure patients. Regional citrate anticoagulation does not affect the coagulation mechanism in vivo, nor does it effects platelets, so in vitro circulating anticoagulants has become the first choice anticoagulant treatment method for continuous renal replacement therapy. Combined with the current research condition at home and abroad, the research progress of the application of regional citrate anticoagulation in artificial liver to treat liver failure is discussed, covering its principle content, application status and application prospect.


Assuntos
Falência Hepática , Fígado Artificial , Lesão Renal Aguda , Anticoagulantes , Citratos , Ácido Cítrico , Heparina , Humanos , Terapia de Substituição Renal
2.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(7): 684-689, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32669161

RESUMO

OBJECTIVE: To compare the efficacy of domestic and imported caffeine citrate in the treatment of apnea in preterm infants. METHODS: A total of 98 preterm infants with a gestational age of 28 - <34 weeks between April 2018 and December 2019 were enrolled. They were randomly administered with domestic (n=48) or imported caffeine citrate (n=50) within 6 hours after birth. The therapeutic effects, complications, adverse effects and clinical outcomes were compared between the two groups. RESULTS: There were no significant differences in the incidence of apnea within 7 days after birth, daily frequency of apnea, the time of apnea disappearance, the failure rate of intubation-surfactant-extubation strategy, the time of non-invasive assisted ventilation, the duration of oxygen therapy, the duration of caffeine citrate therapy, the length of hospital stay, blood gas analysis results, liver and kidney function testing results between the two groups (P>0.05). There were no significant differences in the incidence of complications and the mortality rate between the two groups (P>0.05). There was no significant difference in the incidence of adverse effects between the two groups (P>0.05). CONCLUSIONS: The efficacy and safety of domestic caffeine citrate in the treatment of apnea are similar to those of imported caffeine citrate in preterm infants.


Assuntos
Apneia , Cafeína/uso terapêutico , Citratos/uso terapêutico , Doenças do Prematuro , Apneia/tratamento farmacológico , Método Duplo-Cego , Humanos , Lactente , Recém-Nascido , Recém-Nascido Prematuro , Estudos Prospectivos
3.
J Environ Sci (China) ; 92: 235-244, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32430126

RESUMO

With the release of nanoparticles (NPs) into the subsurface, it is imperative to better understand the fate and transport of NPs in porous media. Three types of stable AuNPs were used as model NPs to investigate the impact of surface coatings (type and coverage) and water velocity on the NP transport in a porous media (column studies). The NPs were electrostatic stabilized citrate AuNPs and sterically stabilized AuNPs with amphiphilic block co-polymer (PVA-COOH) in two particle/polymer ratios (weak vs. strong stabilization). The citrate AuNPs transport was sensitive to ionic changes in the mixing front of the plume, where destabilization occurred, and will therefore depend on the size/type of release. Blocking of deposition sites by aggregates was seen to facilitate transport, whereby a higher flow velocity (larger shadow zone) also resulted in better transport. The polymeric surface coating had great impact with steric repulsion as a main force contributing to the transport of NPs in the porous media. Sufficient polymer coating was crucial to obtain highly unfavorable attachment conditions (very low α) where the enhanced NP mobility was independent of the water velocity (comparable to solute tracer). Without sufficient steric stabilization, the transport and recovery was significantly reduced compared to the solute tracer, but increased with increasing water velocity. This highlights the importance of sufficient surface coating to achieve enhanced mobility, but also the increased risk of spreading to down-gradient receptors. For the (weakly) sterically stabilized NPs, the loss of polymer through ligand exchange with the porous media negates transport.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Citratos , Ácido Cítrico , Ouro , Polímeros , Porosidade , Propriedades de Superfície
4.
Chemosphere ; 255: 126973, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32402889

RESUMO

Ti/SnO2-Sb is a promising anode for electrochemical advanced oxidation process with advantages of low cost and no secondary pollution, while suffers from low work economy due to the short service life. In this study, a facile strategy was proposed to fabricate Ti/SnO2-Sb electrode with high oxidation ability and long service life based on novelly sealing electrodeposited Sn-Sb coating with stannous citrate complex. The treated Ti/SnO2-Sb electrode exhibited an accelerated service life of 41.5 h (100 mA cm-2; 0.5 M H2SO4) and a degradation rate constant for methylene blue dye of 1.02 h-1 which were respectively 11.9 and 2.5 times as that of the untreated electrode. It was found out that the complex could well repair the coating defects inside or outside and form a covering film to tighten the coating, and was then mineralized during the following calcination process to achieve a uniform, rough and highly active SnO2-Sb catalytic layer. The distinctive structure was confirmed by XRD, SEM, XPS and FT-IR. The sealing treatment could be achieved by in situ electrodepositing Sn-Sb coating from or ex situ dipping Sn-Sb coating in solution containing stannous citrate complex followed by drying in air. This study provided a novel, facile and effective strategy to enhance performance of Ti/SnO2-Sb electrode that could be easily achieved in both laboratory and industrial scales and combined with other strategies.


Assuntos
Citratos/química , Modelos Químicos , Compostos de Estanho/química , Catálise , Ácido Cítrico , Eletrodos , Galvanoplastia , Azul de Metileno , Oxirredução , Espectroscopia de Infravermelho com Transformada de Fourier , Titânio/química
5.
Life Sci ; 254: 117785, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32416167

RESUMO

As the most important bioactive substance in Garcinia cambogia, (-)-hydroxycitric acid (HCA) is widely used in food additives to regulate obesity and diabetes in animals or humans, while the mechanism is poorly understood. The purpose of this study was to elucidate the regulatory effect and mechanism of (-)-HCA in regulating glucose and lipid metabolism in chicken primary hepatocytes. The results showed that (-)-HCA obviously decreased triglyceride content through inhibiting the fatty acid synthase protein level, and enhancing the protein level of phosphorylated acetyl CoA carboxylase, enoyl coenzyme A hydratase short chain 1 and carnitine palmitoyltransferase 1A in hepatocytes. Moreover, (-)-HCA markedly enhanced the protein level of phosphofructokinase-1, pyruvate dehydrogenase, succinate dehydrogenase A and complex IV, and which led to the enhancing of glucose uptake and catabolism in hepatocytes. Importantly, the regulation of (-)-HCA on these key factors associated with lipid and glucose metabolism in hepatocytes was mainly achieved through activation of AMP-activated protein kinase/peroxisome proliferator-activated receptor gamma coactivator 1α-nuclear respiratory factor 1 signaling pathway. These results convincingly demonstrated the mechanism of (-)-HCA's regulating on glucose and lipid metabolism, and provided a strategy in prevention of diseases associated with glycolipid metabolic abnormalities in animals, even in humans.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Citratos/farmacologia , Metabolismo Energético/fisiologia , Hepatócitos/metabolismo , Fator 1 Relacionado a NF-E2/metabolismo , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/metabolismo , Acetil-CoA Carboxilase/metabolismo , Animais , Carnitina O-Palmitoiltransferase/metabolismo , Galinhas , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Enoil-CoA Hidratase/metabolismo , Glucose/metabolismo , Fosfofrutoquinase-1/metabolismo , Fosforilação/efeitos dos fármacos , Cultura Primária de Células , Piruvato Desidrogenase (Lipoamida)/metabolismo , Transdução de Sinais/fisiologia , Succinato Desidrogenase/metabolismo , Triglicerídeos/metabolismo
7.
Food Chem ; 317: 126433, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32092613

RESUMO

Highly catalytic and stable N-doped carbon dots (N-CDs) were prepared rapidly by microwave procedure using glucose as precursor and ammonium sulfite as N-dopant. The reduction of AgNO3 by trisodium citrate (TCA) was slow to form nanosilver (AgNP), and the N-CDs exhibited strong catalysis of the AgNP reaction. The formed AgNPs were used as indicator in the presence of Vitoria blue B (VBB) molecule probe with a SERS peak at 1615 cm-1. With the increase of nancatalyst N-CDs concentration, the AgNP reaction speed up, and the SERS peak of VBB enhanced linearly due to formation of more AgNPs as substrate. In the presence of avidin (Ad), the SERS peak weakened. Upon addition of biotin, the SERS peak enhanced due to turn on the indicator nanoreaction. The enhanced SERS signal had a good linear relationship with the biotin concentration in range of 0.0006-0.021 ng/mL, with a detection limit of 0.3 pg/mL.


Assuntos
Biotina/análise , Análise de Alimentos/métodos , Prata/química , Análise Espectral Raman/métodos , Animais , Avidina/química , Carbono/química , Catálise , Citratos/química , Análise de Alimentos/instrumentação , Limite de Detecção , Nanopartículas Metálicas/química , Leite/química , Técnicas de Sonda Molecular/instrumentação , Sondas Moleculares/química , Compostos Orgânicos/química , Pontos Quânticos/química , Nitrato de Prata/química , Análise Espectral Raman/instrumentação
9.
Int J Pharm Compd ; 24(1): 69-76, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32023218

RESUMO

Androgenetic alopecia is the most common form of hair loss. This condition affects both men and women causing significant psychological distress and a decrease in the quality of life. The objective of this study was to investigate the clinical efficacy and patient satisfaction of a topical compounded formulation (minoxidil 10%, finasteride 0.1%, biotin 0.2%, and caffeine citrate 0.05% hydroalcoholic solution) in male androgenetic alopecia patients. A total of five individual, prospective case studies were conducted in the private hair transplant practice of Dr. James C. Marotta. Patients were provided with the topical formulation and instructed to apply a measured 1-mL dose to the entire frontal, parietal, and occipital scalp, twice daily for 6 months. Patients visited the practice periodically (90 days, 120 days, and 180 days post-treatment) for clinical evaluation, photographic assessment, and measurement of their treatment satisfaction by the Men's Hair Growth Questionnaire. By the end of the study, at 180 days, the dermatologist-in-charge concluded that the topical treatment was successful for all five patients. Although moderate, the clinical improvements were visually noticeable as most patients had thicker, more voluminous hair; improved scalp coverage; and improved general hair appearance. These results were consistent with the photographic assessment, which demonstrated a global average increase of +1.05 in the patients' hair density. According to the patients' self-assessment, the topical compounded formulation was effective following 3 months and 6 months of continuous treatment. At 120 days, the patients' satisfaction was neutral or negative, which was likely due to negligible differences in the patients' hair growth and appearance in 90 days compared to 120 days. The results from this study suggest that the new hair-loss topical solution may be considered a safe and effective treatment option in male AGA patients.


Assuntos
Alopecia/fisiopatologia , Biotina/química , Cafeína/química , Citratos/química , Finasterida , Minoxidil , Feminino , Humanos , Masculino , Estudos Prospectivos , Qualidade de Vida , Resultado do Tratamento
10.
Phys Chem Chem Phys ; 22(8): 4788-4792, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32068201

RESUMO

Precipitation and dissolution of calcium oxalate monohydrate (CaOx) crystals are relevant due to their major role in kidney stone diseases. To such an extent, small molecules and ions can act as inhibitors to prevent the formation of calcium oxalate monohydrate crystals. Herein, we explored the role of citrate and the counter cation Na+ ions in the dissolution of CaOx crystals. Citrate binds on the Ca2+ sites of the CaOx crystals to form calcium citrate. Dissolution of CaOx increases with the increase in the concentration of citrate ions and time of incubation. We observed that corrugations were formed on the surface of the CaOx crystals after the sodium citrate treatment during the dissolution process. Theoretical studies revealed that Na+ occupies the vacant site of Ca2+ in CaOx making a strain on the surface which leads to the subsequent deformation of the crystal.


Assuntos
Oxalato de Cálcio/química , Citratos/química , Sódio/química , Cristalização
11.
Exp Parasitol ; 208: 107792, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31707003

RESUMO

Nitazoxanide (NTZ) is a broad-spectrum drug used in intestinal infections, but still poorly explored in the treatment of parasitic tissular infections. This study aimed to evaluate the in vitro responses of the energetic metabolism of T. crassiceps cysticerci induced by NTZ. The organic acids of the tricarboxylic acid cycle, products derived from fatty acids oxidation and protein catabolism were analyzed. These acids were quantified after 24 h of in vitro exposure to different NTZ concentrations. A positive control group was performed with albendazole sulfoxide (ABZSO). The significant alterations in citrate, fumarate and malate concentrations showed the NTZ influence in the tricarboxylic acid (TCA) cycle. The non-detection of acetate confirmed that the main mode of action of NTZ is effective against T. crassiceps cysticerci. The statistical differences in fumarate, urea and beta-hydroxybutyrate concentrations showed the NTZ effect on protein catabolism and fatty acid oxidation. Therefore, the main energetic pathways such as the TCA cycle, protein catabolism and fatty acids oxidation were altered after in vitro NTZ exposure. In conclusion, NTZ induced a significant metabolic stress in the parasite indicating that it may be used as an alternative therapeutic choice for cysticercosis treatment. The use of metabolic approaches to establish comparisons between anti parasitic drugs mode of actions is proposed.


Assuntos
Antiparasitários/farmacologia , Taenia/efeitos dos fármacos , Tiazóis/farmacologia , Albendazol/análogos & derivados , Albendazol/farmacologia , Análise de Variância , Animais , Anti-Helmínticos/farmacologia , Citratos/metabolismo , Ciclo do Ácido Cítrico/efeitos dos fármacos , Meios de Cultura/química , Cysticercus/efeitos dos fármacos , Cysticercus/metabolismo , Metabolismo Energético/efeitos dos fármacos , Fumaratos/metabolismo , Ácidos Cetoglutáricos/metabolismo , Malatos/metabolismo , Neurocisticercose/tratamento farmacológico , Ácido Oxaloacético/metabolismo , Ácido Succínico/metabolismo , Taenia/metabolismo
13.
Cancer Causes Control ; 31(2): 113-126, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31828464

RESUMO

PURPOSE: Alcohol consumption is an established breast cancer risk factor, though further research is needed to advance our understanding of the mechanism underlying the association. We used global metabolomics profiling to identify serum metabolites and metabolic pathways that could potentially mediate the alcohol-breast cancer association. METHODS: A cross-sectional analysis of reported alcohol consumption and serum metabolite concentrations was conducted among 211 healthy women 25-29 years old who participated in the Dietary Intervention Study in Children 2006 Follow-Up Study (DISC06). Alcohol-metabolite associations were evaluated using multivariable linear mixed-effects regression. RESULTS: Alcohol was significantly (FDR p < 0.05) associated with several serum metabolites after adjustment for diet composition and other potential confounders. The amino acid sarcosine, the omega-3 fatty acid eicosapentaenoate, and the steroid 4-androsten-3beta,17beta-diol monosulfate were positively associated with alcohol intake, while the gamma-tocopherol metabolite gamma-carboxyethyl hydroxychroman (CEHC) was inversely associated. Positive associations of alcohol with 2-methylcitrate and 4-androsten-3beta,17beta-diol disulfate were borderline significant (FDR p < 0.10). Metabolite set enrichment analysis identified steroids and the glycine pathway as having more members associated with alcohol consumption than expected by chance. CONCLUSIONS: Most of the metabolites associated with alcohol in the current analysis participate in pathways hypothesized to mediate the alcohol-breast cancer association including hormonal, one-carbon metabolism, and oxidative stress pathways, but they could also affect risk via alternative pathways. Independent replication of alcohol-metabolite associations and prospective evaluation of confirmed associations with breast cancer risk are needed.


Assuntos
Consumo de Bebidas Alcoólicas/sangue , Adulto , Consumo de Bebidas Alcoólicas/metabolismo , Androstenodiol/análogos & derivados , Androstenodiol/sangue , Neoplasias da Mama , Criança , Cromanos/sangue , Citratos/sangue , Estudos Transversais , Dieta , Ácido Eicosapentaenoico/sangue , Feminino , Seguimentos , Humanos , Metabolômica
14.
Sci Total Environ ; 709: 136154, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31884297

RESUMO

Removal of chelated copper from wastewater is more difficult than that of copper ions owing to its stable structure, wide range of pH tolerance, and stronger mobility. Copper citrate (CuCA) widely exists in the water system and inevitably poses serious hazards to human health and environment. Biochar as economic functional material has been widely used for environmental applications, especially in wastewater treatment. This study focused on the performance of manganese oxide-modified biochar (BC-MnOx) toward uptake and removal of CuCA and to understand the related mechanism. The result indicated that the CuCA removal efficiency reached up to 99%. High removal efficiency and low concentration of dissolved Mn over a wide pH range proved that the BC-MnOx is efficient and chemically stable. Furthermore, the removal mechanism may involve the following processes: First, CuCA was removed via the chemical bonds formed between CuCA and MnOx on the surface of BC. Second, chemisorption due to the oxygen-containing functional groups or physisorption of porous structure in BC worked synergistically on CuCA. Third, CuCA was partially oxidized into low molecular weight acids by means of MnOx, while the released Cu ions were retained on the adsorbent surface. This study demonstrates that BC-MnOx is a promising material for the removal of CuCA from wastewater.


Assuntos
Carvão Vegetal , Adsorção , Citratos , Ácido Cítrico , Cobre , Manganês , Compostos de Manganês , Nanoestruturas , Óxidos , Água , Poluentes Químicos da Água , Purificação da Água
15.
J Vet Emerg Crit Care (San Antonio) ; 30(1): 54-59, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31845529

RESUMO

OBJECTIVE: To examine the extent to which rapid thromboelastography (r-TEG) could decrease the testing time in comparison with that required for kaolin-activated thromboelastography (TEG), and to compare 2 types of blood samples (ie, native and citrated whole blood [WB]), for determining r-TEG values in healthy dogs. DESIGN: Prospective observational study. SETTING: University teaching hospital. ANIMALS: Sixteen healthy Beagles. INTERVENTIONS: Kaolin-activated TEG test using citrated WB samples and r-TEG test using native and citrated WB samples were performed in 16 dogs. At 60 minutes after the initial blood sampling, further samples were collected from a subset of 6 dogs in the same manner to evaluate intraindividual repeatability of r-TEG. MEASUREMENTS AND MAIN RESULTS: The mean time to maximum amplitude (MA) for r-TEG with native and citrated WB samples was recorded as 1313.9 ± 250.9 seconds and 1351.3 ± 264.6 seconds (mean ± SD), respectively, and 1779.9 ± 197.0 seconds for kaolin-activated TEG. Coefficients of variation with native and citrated WB samples for r-TEG values, TEG-activated clotting time, clot formation time, α angle, and MA, were determined to be 13.4% versus 18.8%, 11.1% versus 16.6%, 4.2% versus 5.1%, and 10.0% versus 10.0%, respectively. Intraindividual variations were lower for native WB samples than for citrated WB samples. CONCLUSIONS: The r-TEG test significantly decreased the mean time to MA compared with the kaolin-activated TEG test. In addition, native WB samples showed lower coefficients of variation and intraindividual variation than citrated WB samples in r-TEG analysis; this suggests that native WB samples can provide more consistent results. Therefore, the r-TEG method using native WB samples is recommended for assessment of dogs' hemostatic status when an early diagnosis is required.


Assuntos
Doenças do Cão/sangue , Tromboelastografia/veterinária , Animais , Preservação de Sangue/veterinária , Coleta de Amostras Sanguíneas/veterinária , Citratos , Cães , Feminino , Caulim , Masculino , Estudos Prospectivos , Reprodutibilidade dos Testes , Tromboplastina/fisiologia , Tempo de Coagulação do Sangue Total/veterinária
16.
Harefuah ; 158(12): 774-777, 2019 Dec.
Artigo em Hebraico | MEDLINE | ID: mdl-31823528

RESUMO

BACKGROUND: UROCIT-K is a potassium-citrate regimen prescribed for the prevention of kidney stone formation. In 2013, K-CITEK was introduced to the local market as a new potassium-citrate regimen that reduces kidney stone formation in a declared rate of 93. OBJECTIVES: We sought to explore the efficacy of K-CITEK versus UROCIT-K. METHODS: A prospective database of patients treated with potassium-citrate regimens for nephrolithiasis has been reviewed. Patients were divided into two groups: those who were treated with UROCIT-K only (Group 1) and those who were treated with K-CITEK only (Group 2). The two groups were compared as regards to demographics, length of follow-up, urinary citrate level and stone burden changes, as well as the number of stone events (i.e: colic, surgery) throughout the follow-up period. In a separate analysis another group (Group 3) was checked. This group consisted of patients who were initially treated with UROCIT-K and later on were switched to K-CITEK. RESULTS: The study group consisted of 104 patients: 54 patients in Group 1, 38 in group 2 and 12 in group 3. The latter was omitted from analysis due to the small size. Groups 1 and 2 resembled in their demographic data and medical comorbidities. No statistically significant differences were found in terms of change in urinary citrate levels, stone burden or recurrent stone events. CONCLUSIONS: K-CITEK for the treatment of kidney stone prevention was found to be as equally effective as UROCIT-K in terms of increasing urinary citrate levels, reducing stone burden and maintaining the intervals between kidney stone events.


Assuntos
Diuréticos/uso terapêutico , Cálculos Renais/tratamento farmacológico , Citrato de Potássio/uso terapêutico , Citratos , Humanos
17.
Biomed Res Int ; 2019: 8162975, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31662997

RESUMO

Background: Preserved blood cells undergo progressive structural and functional changes that may affect their function, integrity, and viability after transfusion. The impact of transfusion of stored blood on potassium, sodium, or acid-base balance in the recipient may be complex, but information on it is inconsistent. This study therefore sought to determine the changes in the potassium and sodium levels in whole blood stored at 4°C for 28 days and clinical outcomes when such blood are transfused. Methods: Whole blood were taken into double CPDA-1 bags and 50 ml transferred into the satellite bags for the study. Electrolyte concentration determinations were made on each of the blood sample on days 0, 7, 14, 21, and 28 using the Vitalab Selectra Junior chemistry analyser. The remaining blood in the main bags was transfused after the 28-day period, and biochemical analysis carried out on the patients before and after the transfusion. One-way ANOVA was used for the analysis of variance between the weekly ion concentrations and independent sample Mann-Whitney U test for the data obtained from the patients. Results: The mean potassium level of all the samples started with a normal value of 3.45 mmol/L on the first day followed by a sharp rise to 9.40 mmol/L on day 7, 13.40 mmol/L on day 14, 14.60 mmol/L on day 21, and 15.40 mmol/L on day 28. Sodium on the other hand started with a high value of 148.4 mmol/L on day 0 and then reduced to 146.4 mmol/L on day 7, 140.8 mmol/L on day 14, 135.6 mmol/L on day 21, and a low value of 130.8 mmol/L on day 28. No adverse clinical outcomes were seen in patients after they were transfused with the blood. Conclusion: It can be deduced that potassium concentration in refrigerated blood increases, whilst sodium concentration reduces with time and when such blood is transfused, it may not result in any adverse clinical outcome.


Assuntos
Eletrólitos/metabolismo , Íons/sangue , Potássio/sangue , Sódio/sangue , Equilíbrio Hidroeletrolítico/fisiologia , Adenina/metabolismo , Doadores de Sangue , Preservação de Sangue/métodos , Transfusão de Sangue/métodos , Citratos/metabolismo , Glucose/metabolismo , Humanos , Fosfatos/metabolismo
18.
Proc Natl Acad Sci U S A ; 116(44): 21980-21982, 2019 10 29.
Artigo em Inglês | MEDLINE | ID: mdl-31611408

RESUMO

Siderophores, iron-scavenging small molecules, are fundamental to bacterial nutrient metal acquisition and enable pathogens to overcome challenges imposed by nutritional immunity. Multimodal imaging mass spectrometry allows visualization of host-pathogen iron competition, by mapping siderophores within infected tissue. We have observed heterogeneous distributions of Staphylococcus aureus siderophores across infectious foci, challenging the paradigm that the vertebrate host is a uniformly iron-depleted environment to invading microbes.


Assuntos
Sideróforos/análise , Staphylococcus aureus/patogenicidade , Abscesso/microbiologia , Animais , Citratos/análise , Interações Hospedeiro-Patógeno , Ferro/metabolismo , Camundongos , Ornitina/análogos & derivados , Ornitina/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/patologia
19.
Int J Mol Sci ; 20(20)2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31614647

RESUMO

BACKGROUND: Age-related macular degeneration (AMD) is the leading cause of blindness and can be classified into two types called atrophic AMD (dry AMD) and neovascular AMD (wet AMD). Dry AMD is characterized by cellular degeneration of the retinal pigment epithelium, choriocapillaris, and photoreceptors. Wet AMD is characterized by the invasion of abnormal vessels from the choroid. Although anti-vascular endothelial growth factor (VEGF) therapy has a potent therapeutic effect against the disease, there is a possibility of chorio-retinal atrophy and adverse systemic events due to long-term robust VEGF antagonism. We focused on hypoxia-inducible factor (HIF) regulation of VEGF transcription, and report the suppressive effects of HIF inhibition against ocular phenotypes in animal models. Many of the known HIF inhibitors are categorized as anti-cancer drugs, and their systemic side effects are cause for concern in clinical use. In this study, we explored food ingredients that have HIF inhibitory effects and verified their effects in an animal model of AMD. METHODS: Food ingredients were screened using a luciferase assay. C57BL6/J mice were administered the Garcinia cambogia extract (Garcinia extract) and hydroxycitric acid (HCA). Choroidal neovascularization (CNV) was induced by laser irradiation. RESULTS: Garcinia extract and HCA showed inhibitory effects on HIF in the luciferase assay. The laser CNV model mice showed significant reduction of CNV volume by administering Garcinia extract and HCA. Conclusions: Garcinia extract and HCA showed therapeutic effects in a murine AMD model.


Assuntos
Citratos/administração & dosagem , Garcinia cambogia/química , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Degeneração Macular/tratamento farmacológico , Extratos Vegetais/administração & dosagem , Animais , Citratos/química , Citratos/farmacologia , Modelos Animais de Doenças , Regulação para Baixo , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Terapia com Luz de Baixa Intensidade/efeitos adversos , Degeneração Macular/etiologia , Degeneração Macular/genética , Masculino , Camundongos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Resultado do Tratamento
20.
Colloids Surf B Biointerfaces ; 184: 110543, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31627102

RESUMO

In this paper, a bottom-up hydrothermal route is reported for the synthesis of oxygen and nitrogen co-decorated carbon quantum dots (CQDs) using ammonium hydrogen citrate (AHC) as a single precursor. DLS data approved the formation of 4.0 nm (average size) CQDs. XRD pattern shows the interlayer spacing (002) of 3.5 Šfor CQDs, which is exactly the same as that of crystalline graphite. XPS and FTIR spectra verified the formation of oxygen and nitrogen functional groups on the CQDs surface. Co-decorated carboxyl, hydroxyl and amine groups on the CQDs surfaces make them as promising polyelectrolyte for gene delivery. Toxicity assay showed a survival rate of 70% under different incubation times and up to 500 µg/mL. The highly water-soluble, stable fluorescence and low toxic CQDs increased the gene expression of DNA plasmid in E. coli bacteria 4-fold more than the control group.


Assuntos
Carbono/química , DNA/química , Nitrogênio/química , Oxigênio/química , Pontos Quânticos/química , Carbono/farmacologia , Citratos/química , DNA/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Técnicas de Transferência de Genes , Nitrogênio/farmacologia , Oxigênio/farmacologia , Tamanho da Partícula , Plasmídeos , Polieletrólitos/química , Polieletrólitos/farmacologia , Compostos de Amônio Quaternário/química , Propriedades de Superfície
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