Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 12.283
Filtrar
1.
Zhongguo Zhong Yao Za Zhi ; 45(16): 3922-3930, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893590

RESUMO

The aim of this paper was to observe the effect of salvianolic acid B(Sal B) on high-glucose induced renal tubular epithelial-mesenchymal transition(EMT) in rats, and to explore its possible mechanisms of prevention and treatment of diabetic nephropathy. The rat renal tubular epithelial NRK-52 E cells were cultured in vitro. The cells were divided into control group, high glucose group, high glucose+10 µmol·L~(-1)Sal B group(Sal B), the above 3 groups were set at 6, 12, 24 and 48 h for dynamic observation; high glucose+Sal B different concentration(1, 5, 10 µmol·L~(-1)) groups, high glucose+5.0 µmol·L~(-1) pioglitazone group, high glucose+10 µmol·L~(-1)Sal B+5 µmol·L~(-1)GW9662 group. The protein expression levels of PPARγ, PTEN, α-SMA, E-cadherin and PI3 K/Akt signaling molecules were determined by Western blot. The mRNA expression of PPARγ and PTEN were detected by Real-time PCR. The viabi-lity of NRK52 E cells was determined by MTT assay. The results showed that as compared with control group, the mRNA and protein expression levels of PPARγ and PTEN in high glucose group gradually reduced, the protein expression levels of α-SMA and p-Akt~((Thr308))gradually increased, and the protein expression of E-cadherin gradually reduced(P<0.05). As compared with high glucose group, when increases in Sal B doses, the mRNA and protein expression levels of PPARγ, PTEN in high glucose + different concentrations of Sal B groups gradually increased, the protein expression levels of α-SMA and p-Akt~((Thr308)) gradually reduced, and the protein expression of E-cadherin gradually increased(P<0.05), however, the effect of 1 µmol·L~(-1)concentration of Sal B on the expression of PPARγ mRNA and protein and PTEN mRNA was not significantly different. As compared with high glucose group, the mRNA and protein expression levels of PPARγ mRNA(except 6 h) and protein(except 6 h), PTEN mRNA(except 6 h) and protein(except 6, 12 h) kept increasing, the protein expression levels of α-SMA and p-Akt~((Thr308))(except 6 h) continued to reduce, the protein expression of E-cadherin kept increasing in high glucose+10 µmol·L~(-1) Sal B dynamic observation group(P<0.05). As compared with high glucose group, Sal B and the pioglitazone(PIO) can greatly enhance the expression of PPARγ, PTEN at mRNA and protein levels, enhance the expression of E-cadherin at protein levels, and reduce the expression of α-SMA, p-Akt~((Thr308))protein level(P<0.05), there was no significant difference between the two groups. However, the expression levels of PPARγ and PTEN mRNA and protein, E-cadherin, α-SMA and p-Akt(Thr308) protein in the Sal B+GW9662 control group were not statistically significant compared with the high glucose group. The effect of Sal B was blocked by the PPARγ antagonist GW9662. It can be concluded that Sal B can suppress the NRK52 E cells induced by high-glucose EMT. The mechanism may be related to the activation of PPARγ with Sal B, and the up-regulation of PTEN expression, and thereby inhibiting the fibrosis effect of PI3 K/Akt signaling pathway.


Assuntos
Transição Epitelial-Mesenquimal , Fator de Crescimento Transformador beta1 , Animais , Benzofuranos , Células Epiteliais , Glucose , Ratos
2.
Medicine (Baltimore) ; 99(32): e21594, 2020 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-32769914

RESUMO

BACKGROUND: To evaluate the effect of dl-3-N-butylphthalide (NBP) on new cerebral microbleeds (CMBs) in patients with acute ischemic stroke (AIS). METHODS: We will prospectively enroll patients with AIS admitted to the stroke center of Jingjiang People's Hospital. Qualified participants will be randomly assigned to either the NBP group (NBP injection) or the control group (NBP injection placebo) in a ratio of 1:1. Patients will complete the brain magnetic resonance imaging within 48 hours and 14 days after stroke onset to observe the CMBs through susceptibility weighted imaging, and evaluate whether the use of NBP will affect the new CMBs in AIS patients. SPSS 20.0 will be used for statistical analyses. RESULT: We will provide practical and targeted results assessing the safety of NBP for AIS patients, to provide reference for clinical use of NBP. CONCLUSION: The stronger evidence about the effect of NBP on new CMBs in AIS patients will be provided for clinicians.


Assuntos
Benzofuranos/uso terapêutico , Hemorragia Cerebral/tratamento farmacológico , Protocolos Clínicos , Acidente Vascular Cerebral/tratamento farmacológico , Benzofuranos/normas , Hemorragia Cerebral/complicações , Humanos , Isquemia/complicações , Isquemia/tratamento farmacológico , Imagem por Ressonância Magnética/métodos , Inibidores da Agregação de Plaquetas/normas , Inibidores da Agregação de Plaquetas/uso terapêutico , Estudos Prospectivos
3.
Sci Total Environ ; 742: 140424, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-32629249

RESUMO

The Anniston Community Health Survey (ACHS-I) was initially conducted from 2005 to 2007 to assess polychlorinated biphenyl (PCB) exposures in Anniston, Alabama residents. In 2014, a follow-up study (ACHS-II) was conducted to measure the same PCBs as in ACHS-I and additional compounds e.g., polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like non-ortho (cPCBs) substituted PCBs. In this epigenome-wide association study (EWAS), we examined the associations between PCDD, PCDF, and PCB exposures and DNA methylation. Whole blood DNA methylation was measured using Illumina EPIC arrays (n=292). We modeled lipid-adjusted toxic equivalencies (TEQs) for: ΣDioxins (sum of 28 PCDDs, PCDFs, cPCBs, and mPCBs), PCDDs, PCDFs, cPCBs, and mPCBs using robust multivariable linear regression adjusting for age, race, sex, smoking, bisulfite conversion batch, and estimated percentages of six blood cell types. Among all exposures we identified 10 genome-wide (Bonferroni p≤6.74E-08) and 116 FDR (p≤5.00E-02) significant associations representing 10 and 113 unique CpGs, respectively. Of the 10 genome-wide associations, seven (70%) occurred in the PCDDs and four (40%) of these associations had an absolute differential methylation ≥1.00%, based on the methylation difference between the highest and lowest exposure quartiles. Most of the associations (six, 60%) represented hypomethylation changes. Of the 10 unique CpGs, eight (80%) were in genes shown to be associated with dioxins and/or PCBs based on data from the 2019 Comparative Toxicogenomics Database. In this study, we have identified a set of CpGs in blood DNA that may be particularly susceptible to dioxin, furan, and dioxin-like PCB exposures.


Assuntos
Benzofuranos , Dioxinas , Bifenilos Policlorados/análise , Alabama , Metilação de DNA , Dibenzofuranos Policlorados , Seguimentos , Saúde Pública , Inquéritos e Questionários
4.
Sci Total Environ ; 744: 140710, 2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-32712415

RESUMO

The main objective of the present research was to evaluate the levels of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (dl-PCBs) in the human milk of Spanish lactating women who participated in the BETTERMILK project so that exposure and risk could be assessed for these mothers and their breastfed children. The total ∑PCDD/Fs + dl-PCBs TEQ2005 concentrations in the upper-bound (UB) ranged from 1.29 to 13.48 pgTEQ2005 g-1 lipid. The estimated geometric mean level for the sum of PCDD/F and dl-PCBs was 4.10 and 4.42 pgTEQ2005 g-1 lipid, lower-bound (LB) and UB respectively and were below the reference level reported by EFSA, which is associated with adverse effects in boys of 9 years and related to lactating mothers' exposure values of the tolerable weekly intake (TWI) of around 2 pgTEQ2005 kg-1 bw per week. Nevertheless, it was exceeded when the 95th percentile (8.31 pgTEQ2005 g-1 lipid, as UB) was considered. Results from a multiple regression analysis showed that age has a significant impact on milk ∑PCDD/Fs + dl-PCBs levels, with higher concentrations observed in the milk from older mothers.


Assuntos
Benzofuranos/análise , Dioxinas , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas/análise , Animais , Monitoramento Biológico , Criança , Dibenzofuranos , Dibenzofuranos Policlorados , Feminino , Humanos , Lactação , Masculino , Leite Humano/química , Mães , Medição de Risco , Espanha
5.
Pestic Biochem Physiol ; 168: 104624, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32711764

RESUMO

Fraxinellone (FRA) is a degraded limonoid isolated from the root bark of Dictamnus plants. The potent insecticidal activity of FRA has led to the synthesis of numerous derivatives (presented here with the structure-activity relationships) active against the oriental armyworm Mythimna separata Walker. In addition to its pesticidal activity, the natural product displays potent anti-inflammatory and immuno-modulatory effects at the origin of hepatoprotective and anticancer properties. This mini-review provides an update of the mechanism of action of FRA to highlight the recently discovered capacity of the compound to deactivate cancer-associated fibroblasts and thus to limit the immunosuppressive tumor microenvironment. The anticancer mode of action of FRA raises new ideas to better understand its primary insecticidal activity. The relationship between drug-induced cancer cell death and insect cell death is discussed. A drug interaction with the insect cytokine growth-blocking peptide (GBP), a member of the large EGF family, is proposed, supported by preliminary molecular modeling data. Altogether, the review shed light on the pharmacological properties of fraxinellone as an antitumor agent and a natural insecticide.


Assuntos
Benzofuranos , Inseticidas , Mariposas , Animais , Larva
6.
Medicine (Baltimore) ; 99(28): e21036, 2020 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32664111

RESUMO

BACKGROUND: Liver fibrosis is a pathological change existing in most chronic liver diseases, which leads to abnormal changes in liver tissue structure and affects the normal physiological function of liver. Without effectively control, liver fibrosis can develop into cirrhosis and increase the risk of liver cancer. Salvianolic acid B (Sal B) is the main active component in the water-soluble extract from Salvia miltiorrhiza, which is a traditional Chinese medicine usually used for treating cardiovascular and liver diseases. It is reported that Sal B shown a good action against liver fibrosis via numerous signaling pathways, which indicate that Sal B is a potential candidate drug for the treatment of liver fibrosis. METHODS: We searched the related researches from the following electronic databases: PubMed, EMBASE, Web of science, China National Knowledge Infrastructure (CNKI), China Biology Medicine (CBM), Wan fang Database for Chinese Technical Periodicals and VIP Database. All the databases were searched from inception to December 2019. No restriction of language, publication date, or publication status. PICO of this systematic review are shown as flowing: P, preclinical studies which evaluated the effects of Sal B on the animal models of liver fibrosis with controlled studies; I, received Sal B as only treat in any dose; C, received normal saline, distilled water, or no treatment; O, the primary outcome include measure will be the decrease in liver fibrosis score, and the secondary outcomes include the index of liver fibrosis. All the included data will be analyzed with the software of Review Manager 5.2 and STATA 14.2. DISCUSSION: The purpose of this study is to conduct a systematic review and meta-analysis to assess the effects on anti-liver fibrosis of Sal B, and this will be contribute to drug development and pathological mechanisms of clinical research. TRIAL REGISTRATION: INPLASY202050101, registered on 28/5/2020.


Assuntos
Benzofuranos/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Cirrose Hepática/tratamento farmacológico , Projetos de Pesquisa , Animais , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Humanos , Índice de Gravidade de Doença
7.
Zhongguo Zhong Yao Za Zhi ; 45(12): 2960-2965, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32627473

RESUMO

The aim of this paper was to investigate whether the mechanism of salvianolic acid B in protecting H9 c2 cardiomyocytes from hypoxia/reoxygenation injury is related to the regulation of mitochondrial autophagy mediated by NIX. H9 c2 cardiomyocytes were cultured in vitro and divided into normal group, model group and salvianolic acid B group(50 µmol·L~(-1)). Hypoxia/reoxygenation injury model was established by hypoxia for 4 h and reoxygenation for 2 h. In normal group, high glucose DMEM medium was used for culture. Those in model group were cultured with DMEM medium without glucose and oxygen, and no drugs for hypoxia and reoxyge-nation. In salvianolic acid B group, salvianolic acid B prepared by glucose-free DMEM medium was added during hypoxia, and the other process was as same as the model group. The cell viability was evaluated by CCK-8 assay. The leakage of lactate dehydrogenase(LDH) was detected by microplate method. The levels of intracellular reactive oxygen species(ROS) and mitochondrial membrane potential(ΔΨm) were measured by chemical fluorescence method. The level of intracellular adenosine triphosphate(ATP) was mea-sured by fluorescein enzyme method. The autophagy related proteins LC3-Ⅰ, LC3-Ⅱ, apoptosis related protein cleaved caspase-3 and mitochondrial autophagy receptor protein NIX were detected by Western blot. As compared with the normal group, the activity of H9 c2 cardiomyocytes and ATP level were decreased(P<0.05); LDH leakage and ROS production were increased(P<0.01); ΔΨm was decreased(P<0.01); LC3-Ⅱ/LC3-Ⅰ ratio, cleaved caspase-3 and NIX protein expression levels were increased(all P<0.05) in the model group. As compared with the model group, the activity of cells and ΔΨm were significantly increased(P<0.01); ATP level was increased(P<0.05); LDH leakage and ROS generation were decreased(P<0.01); LC3-Ⅱ/LC3-Ⅰ ratio was decreased(P<0.01); cleaved caspase-3 and NIX expression levels were decreased(P<0.05) in the salvianolic acid B group. The protective effect of salvianolic acid B on hypoxia/reoxygenation injury of H9 c2 cardiomyocytes may be associated with inhibiting mitochondrial auto-phagy. The specific mechanism may be related to inhibiting the activation of mitochondrial autophagy mediated by NIX, increasing ΔΨm, reducing ROS production, reducing the expression of cleaved caspase-3, LC3-Ⅱ, and increasing cell viability.


Assuntos
Autofagia , Miócitos Cardíacos , Apoptose , Benzofuranos , Hipóxia Celular , Sobrevivência Celular , Humanos , Hipóxia
8.
J Asian Nat Prod Res ; 22(10): 947-955, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32567953

RESUMO

A new approach for the total synthesis of the active stilbene dimer dehydro-δ-viniferin has been achieved in 9 steps with methyl 4-hydroxybenzoate and 3,5-dihydroxyacetophenone as starting materials. The key feature of the method is the amberlyst 15-mediated cyclodehydration of α-aryloxyketone. [Formula: see text].


Assuntos
Benzofuranos , Estilbenos , Estrutura Molecular , Resorcinóis , Resveratrol
9.
J Toxicol Sci ; 45(6): 339-347, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32493876

RESUMO

The metabolomic profiles of rat primary hepatocytes following treatment with rotenone, FCCP, or (+)-usnic acid were determined using liquid chromatography-mass spectrometry/mass spectrometry and gas chromatography-mass spectrometry. Significant and similar changes in the levels of 283 biochemical metabolites were associated with the three treatments compared with solvent control samples. Overall, the three treatments generated similar global biochemical profiles, with some minor differences associated with rotenone treatment. All three treatments resulted in a shift in energy metabolism as demonstrated by decreased glycogen stores and glycolysis. A reduced antioxidant response was detected in cells following all treatments. In addition, bile acid biosynthesis decreased as a potential consequence of increased oxidative stress by all three treatments. Conversely, rotenone treatment induced a number of changes after 1 hr, which were not detected in FCCP- or (+)-usnic acid-treated samples; these changes were not sustained over time and included increased NAD+ salvage and lysine degradation. In conclusion, these biochemical profiles could provide new insights into the mechanism(s) of mitochondrial toxicity.


Assuntos
Benzofuranos/efeitos adversos , Carbonil Cianeto p-Trifluormetoxifenil Hidrazona/efeitos adversos , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Rotenona/efeitos adversos , Animais , Ácidos e Sais Biliares/metabolismo , Células Cultivadas , Cromatografia Líquida , Metabolismo Energético/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Glicogênio/metabolismo , Glicólise/efeitos dos fármacos , Metabolômica , Mitocôndrias Hepáticas/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ratos Endogâmicos F344
10.
Sci Total Environ ; 737: 139520, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32531508

RESUMO

Legal restrictions and bans have led to a steady decrease in PCB environmental concentrations. Yet, in recent years PCBs have been found at very high levels in the Mediterranean Sea, for instance, in some apex predators. This work aimed to investigate current PCB (eighteen congeners: #28,52,77,81,101,105,114,118,123,126,138,153,156,157,167,169,180,189) concentrations in the Mediterranean Sea and their relevance today, focusing on their occurrence in edible fish species typically consumed in the Mediterranean diet. In spring 2017, a total of 48 fish samples from the Northern Thyrrenian Sea were collected: 16 specimens of sardine (Sardina pilchardus), 16 of anchovy (Engraulis encrasicolus) and 16 of bogue (Boops boops). PCBs were quantified in the muscle of the animals by means of GC-QqQ-MS. They were found in all samples at the greatest concentrations (ng/g w.w.) in sardine (4.15-17.9, range), and very similar values between anchovy (1.01-7.08) and bogue (1.46-7.22). WHO-TEQ PCB values followed the same order, i.e. sardine (0.410-1.24, range in pg/g w.w.) > anchovy (0.0778-0.396) ~ bogue (0.0726-0.268). These concentrations lied below the European limits of 75 ng/g (w.w.) for the six indicator PCBs and 6.5 pg/g WHO-TEQ for dioxins and dioxin-like PCBs in muscle meat of fish. Additionally, estimated weekly intakes (EWI, in pg WHO-TEQ/Kg/week) for sardine (1.2), anchovy (0.29) and bogue (0.35) scored below the safe value proposed by EFSA of 2 pg WHO-TEQ/Kg/week. When comparing with data reported for the same species in previous Mediterranean studies, values found here were lower than those surveyed in the late 90s and early 2000s; however, they were often not notably different from concentrations reported in last years. This builds up on the concept of a current slow decrease of PCBs in the Mediterranean Sea, likely linked to new inputs and/or remobilization of burdens, and reinforces the need of continous monitoring of these legacy contaminants still ubiquitous today.


Assuntos
Benzofuranos/análise , Dioxinas/análise , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas/análise , Animais , Contaminação de Alimentos/análise , Mar Mediterrâneo
11.
Gene ; 756: 144920, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32593720

RESUMO

Basic helix-loop-helix (bHLH) transcription factors play essential roles in myriad regulatory processes, including secondary metabolism. In this study with Salvia miltiorrhiza, we isolated and characterized SmbHLH53, which encodes a bHLH family member. Expression of this gene was significantly induced by wounding and multiple hormones, including methyl jasmonic acid; transcript levels were highest in the leaves and roots. Phylogenetic analysis indicated that SmbHLH53 clusters withAtbHLH17 and AtbHLH13, two negative regulators of jasmonate (JA) responses, and is localized in the nucleus and cell membrane. Yeast two-hybrid and bimolecular fluorescent complementation assays indicated that SmbHLH53 forms a homodimer as well as a heterodimer with SmbHLH37. It also interacts with both SmJAZs1/3/8 and SmMYC2, the core members of the JA signal pathway. Unexpectedly, we noted that overexpression of SmbHLH53 did not significantly influence the concentrations of rosmarinic acid and salvianolic acid B in transgenic plants. Results from yeast one-hybrid assays showed that SmbHLH53 binds to the promoters of SmTAT1, SmPAL1, and Sm4CL9, the key genes for enzymes in the pathway for phenolic acid synthesis. Assays of transient transcriptional activity demonstrated that SmbHLH53 represses the promoter of SmTAT1 while activating the promoter of Sm4CL9. Thus, the present work revealed that SmbHLH53 may play dual roles in regulating the genes for enzymes in the pathway for Sal B biosynthesis.


Assuntos
Benzofuranos/metabolismo , Salvia miltiorrhiza/genética , Salvia miltiorrhiza/metabolismo , Transdução de Sinais , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/análise , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Vias Biossintéticas , Núcleo Celular/química , Ciclopentanos/metabolismo , Oxilipinas/metabolismo , Filogenia , Proteínas de Plantas/análise , Proteínas de Plantas/metabolismo , Regiões Promotoras Genéticas , Mapas de Interação de Proteínas , Multimerização Proteica , Salvia miltiorrhiza/enzimologia , Metabolismo Secundário
12.
Chemosphere ; 257: 127137, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32480086

RESUMO

Polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) and polychlorinated biphenyls (PCBs) are persistent organic pollutants that have detrimental health effects. As people are exposed to them mainly through the diet, EU has set maximum food dioxin and PCBs levels. EFSA CONTAM Panel made new risk assessment in 2018 that lowered the tolerable weekly intake (TWI) from 14 pg-TEQ/kg bw/week to 2 pg-TEQ/kg bw/week. Critical effect was decreased semen count at the age of 18-19 years if serum total TEQ at the age of 9 years exceeded the No Observed Adverse Effect Level (NOAEL) of 7 pg/g lipid. However, it is largely unknown to what extent NOAEL is exceed in European boys currently. We thus measured PCBs from small volume of serum in 184 Finnish children 7-10 years of age. To estimate the TEQ levels of children from measured PCB levels, we used our existing human milk PCDD/F and PCB concentrations to create a hierarchical Bayesian regression model that was used to estimate TEQs from measured PCBs. For quality control (QC), three pooled blood samples from 18 to 20 year old males were measured for PCDD/Fs and PCBs, and estimated for TEQs. In QC samples measured and estimated TEQs agreed within 84%-106%. In our estimate for 7-10 year old children, PCDD/F TEQ exceeded NOAEL only in 0.5% and total TEQ in 2.7% of subjects. Risk management following the decreased TWI proposed by the CONTAM Panel should be carefully considered if total TEQ in children is already largely below the NOAEL.


Assuntos
Dibenzofuranos Policlorados/sangue , Exposição Ambiental/análise , Poluentes Ambientais/sangue , Dibenzodioxinas Policloradas/sangue , Adolescente , Adulto , Teorema de Bayes , Benzofuranos/análise , Criança , Dibenzofuranos , Dibenzofuranos Policlorados/análise , Dieta , Dioxinas/análise , Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/análise , Finlândia , Contaminação de Alimentos/análise , Humanos , Leite Humano/química , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas/análise , Medição de Risco , Adulto Jovem
13.
Environ Sci Pollut Res Int ; 27(27): 34172-34181, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32557054

RESUMO

Coal-fired power plants are characterized by high combustion temperature and well-equipped air pollution control devices. The trace organic pollutants in the municipal solid waste incineration (MSWI) flue gas would be completely destroyed if the MSWI flue gas was injected into the high temperature area of a coal-fired boiler. In this study, the emission characteristics of common gas pollutants, heavy metals, and dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in both flue gas and bottom ash when the MSWI flue gas was injected into a lab-scale coal-fired drop-tube furnace were investigated. After co-processing, the concentrations of NOx, SO2, CO, and all heavy metals in the flue gas emitted from the coal-fired drop-tube furnace did not change a lot. However, the concentration of HCl in the flue gas from drop-tube furnace increased after coupling the MSWI flue gas. Moreover, the I-TEQ values of the PCDD/Fs in the flue gas and bottom ash after coupling the MSWI flue gas were 0.037 ng I-TEQ/Nm3 and 0.63 ng I-TEQ/g, respectively. The main formation pathways of PCDD/Fs in the flue gas of drop-tube furnace were suggested to be de novo synthesis and precursor synthesis. Furthermore, the effects of oxygen content and temperature on the formation of PCDD/Fs were also studied. The reduction efficiencies of the total amount of PCDD/Fs in the flue gas from the co-processing system were more than 60%, and even reached 90%. Therefore, co-processing of the MSWI flue gas in coal-fired power plants might be an environmentally friendly technology.


Assuntos
Poluentes Atmosféricos/análise , Benzofuranos/análise , Dibenzodioxinas Policloradas/análise , Carvão Mineral , Cinza de Carvão , Dibenzofuranos , Dibenzofuranos Policlorados , Incineração , Resíduos Sólidos/análise
14.
Sci Total Environ ; 738: 139670, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-32534283

RESUMO

This study was performed to evaluate the use of white rot fungus, Pleurotus pulmonarius, to treat polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) in contaminated soil using solid state fermentation (SSF). The soil was collected from a long-closed pentachlorophenol plant in southern Taiwan. The non-sterilized soil with a total PCDD/F concentration of 14,000 ±â€¯2400 ng I-TEQ kg-1 was mixed directly with the solid fungal inocula at dry w/w ratio of 1:1.4 (ratio-adjusted test) and incubated at 26 ±â€¯2 °C in a controlled environment. The highest PCDD/F decomposition was observed during the mycelium colonization. Pearson correlation coefficient (r) studied during this period (35 days) indicated that laccase had no significant correlation (r = -0.53), while manganese peroxidase had a strong positive correlation (r = 0.88) with PCDD/F decomposition efficiency. After 72 days, the more toxic congeners, tetra- and penta-CDD/Fs were removed to non-detectable levels. Meanwhile, the removal efficiencies of hexa-, hepta-, and octa-CDD/Fs were >80%, >97%, and >90%, respectively. The simultaneous degradation of low and high chlorinated DD/Fs suggested that overall removal was nonspecific. The overall PCDD/F removal was 96%, and the residual concentration (276 ng I-TEQ kg-1) was below the regulatory control limit (1000 ng I-TEQ kg-1). In conclusion, this study shows that P. pulmonarius via SSF can successfully remediate the PCDD/F-contaminated field soil. Furthermore, this SSF technique overcame the well-known intractability of PCDD/F biodegradation in non-sterilized soil, making it promising for actual field application.


Assuntos
Benzofuranos/análise , Pleurotus , Dibenzodioxinas Policloradas/análise , Poluentes do Solo/análise , Biodegradação Ambiental , Dibenzofuranos , Dibenzofuranos Policlorados , Fermentação , Solo , Taiwan
15.
Environ Pollut ; 265(Pt B): 114888, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32554086

RESUMO

The emission of polychlorinated dibenzo-p-dioxins and -furans (PCDD/F) from full-scale municipal solid waste incinerators (MSWI) is harmful to human and environmental health. This study analyzes the effect of different units of an air pollution control devices (APCDs), i.e. the semi-dry scrubber, fabric filter (FF), selective catalytic reduction (SCR), and wet scrubber (WS), on the removal characteristics and gas- and solid-phase distributions of PCDD/F in MSWI flue gas. APCDs reduce PCDD/F concentrations from 24.9 ng Nm-3 to 0.979 ng Nm-3 (2.16 ng I-TEQ Nm-3 to 0.0607 ng I-TEQ Nm-3), with a total removal efficiency (RE) of 96.1% (97.2% I-TEQ). Specifically, APCDs remove more than 95% of both gas- and solid-phase PCDD/F. The FF coupled with active carbon injection (FF + ACI) substantially reduces both gas- and solid-phase PCDD/F concentrations with an RE of 97.2% (98.7% I-TEQ). Additionally, FF + ACI exhibits a better RE of PCDF (98.9%) than PCDD (94.6%) and leads to PCDD congeners dominating the gas-phase. Both desorption and destruction of PCDD/F occur in the SCR, which favors removal of gas-phase PCDD/F but increases solid-phase PCDD/F. Therefore, SCR only decreases PCDD/F with a low RE of 27.6% (16.9% I-TEQ). However, SCR reduces NOx with a high RE of 82.3%, which could inhibit the RE of PCDD/F because of their different reaction mechanisms. WS increases PCDD/F in both the gas and solid-phase by 1.95 times (2.57 times for I-TEQ) due to the memory effect, which typically increases the total mass concentration of PCDD/F and the proportions of lower-chlorinated gas-phase PCDD/F. Migration of gas- and solid-phase PCDD/F are also analyzed according to temperature. The results of this study can contribute to the optimized design of industrial APCDs for controlling PCDD/F emissions from MSWI.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/análise , Benzofuranos/análise , Dibenzodioxinas Policloradas/análise , Dibenzofuranos Policlorados , Monitoramento Ambiental , Incineração , Resíduos Sólidos
16.
Environ Pollut ; 265(Pt B): 114957, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32554089

RESUMO

Although huge interspecies differences in the response to dioxins have been acknowledged, toxic equivalency factors derived from rodent studies are often used to assess human health risk. To determine interspecies differences, we first developed a toxicokinetic model in humans by measuring dioxin concentrations in environmental and biomonitoring samples from Southern China. Significant positive correlations between dioxin concentrations in blood and age were observed for seven dioxin congeners, indicating an age-dependent elimination rate. Based on toxicokinetic models in humans, the half-lives of 15 dioxin congeners were estimated to be 1.60-28.55 years. In consideration that the highest contribution to total toxic equivalency in blood samples was by 12378-polychlorinated dibenzo-p-dioxin (P5CDD), this study developed a physiologically based pharmacokinetic (PBPK) model of 12378-P5CDD levels in the liver, kidney, and fat of C57/6J mice exposed to a single oral dose, and the half-life was estimated to be 26.1 days. Based on estimated half-lives in humans and mice, we determined that the interspecies difference of 12378-P5CDD was 71, much higher than the default usually used in risk assessment. These results could reduce the uncertainty human risk assessment of 12378-P5CDD, and our approach could be used to estimate the interspecies differences of other dioxin congeners.


Assuntos
Benzofuranos , Dioxinas , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas , Animais , China , Dibenzofuranos Policlorados , Humanos , Camundongos , Especificidade da Espécie
17.
Medicine (Baltimore) ; 99(20): e20151, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32443330

RESUMO

BACKGROUND: This study will specifically investigate the effect of butylphthalide on hemodynamics in patients with watershed stroke (WS). METHODS: We will search the following databases from their inceptions to the March 1, 2020: Cochrane Library, MEDLINE, EMBASE, PsycINFO, Web of Science, Cumulative Index to Nursing and Allied Health Literature, and China National Knowledge Infrastructure. All relevant randomized controlled trials on exploring the effect of butylphthalide on hemodynamics in patients with WS will be considered for inclusion. No language limitation will be imposed to this study. All study quality will be checked using Cochrane risk of bias tool. RevMan 5.3 software will be utilized for data analysis. RESULTS: This study will summarize the latest evidence to investigate the effect of butylphthalide on hemodynamics in patients with WS. CONCLUSION: Findings from this study will provide theoretical basis of butylphthalide on hemodynamics in patients with WS for clinician and future research. DISSEMINATION AND ETHICS: This study is carried out based on the published data, thus, no ethical approval is required. We will submit this study to a peer-reviewed journal for publication. SYSTEMATIC REVIEW REGISTRATION: INPLASY 202030006.


Assuntos
Benzofuranos/farmacologia , Hemodinâmica/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Acidente Vascular Cerebral/tratamento farmacológico , Benzofuranos/administração & dosagem , Benzofuranos/efeitos adversos , China/epidemiologia , Humanos , Fármacos Neuroprotetores/administração & dosagem , Fármacos Neuroprotetores/efeitos adversos , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Acidente Vascular Cerebral/patologia , Acidente Vascular Cerebral/fisiopatologia , Resultado do Tratamento
18.
Biol Res ; 53(1): 19, 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32366289

RESUMO

BACKGROUND: Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The aim of this study was to determine the effect of usnic acid response-miR-185-5p on proliferation in the breast cancer cell and to determine its relationship with apoptosis pathway. METHODS: The cell proliferation and cell apoptosis rate were significantly increased following the ectopic expression of miR-185-5p in BT-474 cells. Furthermore, the results of cell cycle assay performed by flow cytometry revealed that the transfection with miR-185-5p induced G1/S phase arrest. The apoptosis-related genes expression analysis was performed by qRT-PCR and the direct target of miR-185-5p in BT-474 cells was identified by western blot and luciferase reporter assay. RESULTS: Our data showed that miR-185-5p can cause significant changes in apoptosis-related genes expression levels, suggesting that cell proliferation was suppressed by miR-185-5p via inducing apoptosis in breast cancer cells. According to western blot results, miR-185-5p lead to decrease BCL2 protein level in BT-474 cells and direct target of miR-185-5p was identified as BCL by luciferase reporter assay. CONCLUSION: This study revealed that miR-185-5p may be an effective agent in the treatment of breast cancer.


Assuntos
Benzofuranos/metabolismo , Neoplasias da Mama/genética , MicroRNAs/genética , Proteínas Proto-Oncogênicas c-bcl-2/genética , Apoptose , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Regulação para Baixo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais , Transfecção
19.
Chemosphere ; 256: 127065, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32454353

RESUMO

Dibenzo-p-dioxin (DD) and dibenzofuran (DF) chlorination mediated by Cu and Fe chlorides can make a direct contribution to the formation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in combustion flue gas. In this study, a kind of composite Cu and Fe chlorides and oxides (CuFe9O9.5Cl10) was prepared by impregnating oxides with HCl solution to imitate the coexistence status of Cu and Fe species in combustion flue gas. Composite CuFe9O9.5Cl10 was active in promoting the electrophilic chlorination of DD/DF at 150-300 °C, with the highest activity at 200 °C. DD/DF chlorination could occur under inert atmosphere, and 5% O2 atmosphere was most favorable for DD/DF chlorination. Electrophilic chlorination of DD/DF primarily favored at 2,3,7,8 positions. Hybridization of Cu and Fe chlorides and oxides not only decreased the starting temperature and activation energy of DD/DF chlorination, but also induced a synergistic effect for accelerating the chlorination of DD/DF. The measured activities of composite CuFe9O9.5Cl10 for promoting the chlorination of DD/DF were near to those of composite Cu chloride and oxide (CuO0.2Cl1.6), whereas 2 orders of magnitude higher than those of composite Fe chloride and oxide (FeO0.3Cl2.4). Comparison of PCDD/F congener distribution patterns indicated that DD/DF chlorination should be a main source of Cl1-3DFs and Cl1-2DDs in combustion flue gases.


Assuntos
Benzofuranos/química , Cobre/química , Dioxinas/química , Dibenzodioxinas Policloradas/química , Cloretos , Cloro , Dibenzofuranos , Dibenzofuranos Policlorados , Halogenação , Incineração , Ferro , Óxidos , Temperatura
20.
Chemosphere ; 256: 127023, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32428742

RESUMO

Persistent organic pollutants (POPs) methods for foods and animal feeds require sufficient sample intake followed by an extensive removal of interfering matrix components and concentration before a gas chromatographic mass spectrometry (GC-MS) method can be applied. The extraction dissolves associated lipids in animal foods or feeds. Methods must eliminate all co-extracted lipids before determination by GC-MS. A new approach for removing lipids is presented using basic silica gel or metal ion immobilized silica gel (Ag+) in a single step. Absorbent order, adsorbent amounts, and flow rates were found to be essential for consistent results. KOH/silica gel or Ag+ ion (AgNO3) silica gel were both shown to retain 75-85% of the co-extracted lipids without using sulfuric acid. KOH/silica gel method applied to butter fortified at 7.3 pg TEQ/g lipid with polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) produced accurate results for all fortified congeners with 20% of predicted (n = 6). Ag+ silica gel incorporated into the Miura GO-EHT automated system produced similar results fortified at 3 pg TEQ/g lipid. During PCDD/F fortifications of butter with PCDD/Fs (n = 6), labeled standard recoveries for PCDD/Fs and planar polychlorinated biphenyls (PCBs) were all acceptable (52-99%) averaging 77% using the Miura system. A reduction in the amounts of sulfuric acid silica gel needed was possible in the completion of co-extractant removal. PCDD/F spikes into butter and for a spiked sunflower oil (PCDD/Fs and coplanar PCBs) were within ± 20% of the predicted using the Miura system; suitable for current methods criteria for foods including criteria in EU legislation.


Assuntos
Poluentes Ambientais/análise , Contaminação de Alimentos/análise , Animais , Benzofuranos/análise , Manteiga/análise , Dibenzofuranos/análise , Dibenzofuranos Policlorados/análise , Cromatografia Gasosa-Espectrometria de Massas , Lipídeos/análise , Espectrometria de Massas , Bifenilos Policlorados/análise , Dibenzodioxinas Policloradas/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA