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1.
Colloids Surf B Biointerfaces ; 171: 115-122, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30025373

RESUMO

The aim of the work was to extract, characterize, and formulate Thymus capitatus (Tymbra capitata) essential oil in phospholipid vesicles: liposomes, glycerosomes and Penetration Enhancer-containing Vesicles (PEVs). The steam-distilled essential oil was mainly composed of carvacrol. The oil was mixed with lecithin and water to produce liposomes, or different ratios of water/glycerol or water/propylene glycol (PG) to produce glycerosomes and PG-PEVs, respectively. Cryo-TEM showed the formation of unilamellar, spherical vesicles, and light scattering disclosed that their size increased in the presence of glycerol or PG, which improved long-term stability. The formulations were highly biocompatible, and capable of counteracting oxidative stress and favouring wound repair in keratinocytes, thanks to enhanced uptake. The antibacterial activity of the oil was demonstrated against cariogenic Streptococcus mutans, Lactobacillus acidophilus, and commensal Streptococcus sanguinis. The combination of antioxidant and antibacterial activities of Thymus essential oil formulations may be useful for the treatment of oral cavity diseases.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Doenças da Boca/tratamento farmacológico , Doenças da Boca/microbiologia , Óleos Voláteis/farmacologia , Fosfolipídeos/química , Extratos do Timo/farmacologia , Thymus (Planta)/química , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Humanos , Queratinócitos/efeitos dos fármacos , Lactobacillus/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Tamanho da Partícula , Streptococcus/efeitos dos fármacos , Propriedades de Superfície , Extratos do Timo/química , Extratos do Timo/isolamento & purificação
2.
Hum Mol Genet ; 26(4): 729-741, 2017 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-28115417

RESUMO

TMEM230 is a newly identified Parkinson's disease (PD) gene encoding a transmembrane protein whose cellular and pathogenic roles remain largely unknown. Here, we demonstrate that loss of TMEM230 disrupts retromer cargo CI-M6PR (cation-independent mannose 6-phosphate receptor) trafficking and autophagic cargo degradation rates. TMEM230 depletion further inhibits extracellular secretion of the autophagic cargo p62 and immature lysosomal hydrolases in Golgi-derived vesicles leading to their intracellular accumulation, and is specifically mediated by loss of the small GTPase Rab8a. Importantly, PD-linked TMEM230 variants also induce retromer mislocalization, defective cargo trafficking, and impaired autophagy. Finally, we show that knockdown of another PD gene, LRRK2, which phosphorylates Rab8a, similarly impairs retromer trafficking, secretory autophagy and Golgi-derived vesicle secretion, thus demonstrating converging roles of two PD genes TMEM230 and LRRK2 on Rab8a function, and suggesting that retromer and secretory dysfunction play an important role in PD pathogenesis.


Assuntos
Complexo de Golgi/metabolismo , Proteínas de Membrana/metabolismo , Doença de Parkinson/metabolismo , Vesículas Secretórias/metabolismo , Proteínas rab de Ligação ao GTP/metabolismo , Animais , Células COS , Chlorocebus aethiops , Complexo de Golgi/genética , Células HEK293 , Humanos , Serina-Treonina Proteína Quinase-2 com Repetições Ricas em Leucina/genética , Serina-Treonina Proteína Quinase-2 com Repetições Ricas em Leucina/metabolismo , Proteínas de Membrana/genética , Doença de Parkinson/genética , Fosforilação/genética , Vesículas Secretórias/genética , Extratos do Timo , Proteínas rab de Ligação ao GTP/genética
3.
Vopr Virusol ; 61(2): 74-8, 2016.
Artigo em Russo | MEDLINE | ID: mdl-27451499

RESUMO

The goal of this work was the evaluation of the frequency of human CMV infection among the women, whose pregnancy ended in miscarriage, detection of active forms of infection and treatment before pregnancy. Virological and sero-immunological techniques were used. A total of 116 women who had miscarriages before the 28 week of pregnancy were submitted to the CMV test. 109 women (94.0%) demonstrated positive results. 49 women (42.2%) had active form of the cytomegalovirus infection. 13 women (26.5%) had the recurrent form and 36 patients (73.5%) had the persistent form of CMV infection (stage of productive replication). All the women with active CMVI were treated before the next pregnancy. Immunomodulatory therapy for the treatment was used.


Assuntos
Aborto Habitual/diagnóstico , Anticorpos Antivirais/sangue , Antígenos Virais/sangue , Infecções por Citomegalovirus/diagnóstico , Citomegalovirus/crescimento & desenvolvimento , Complicações Infecciosas na Gravidez/diagnóstico , Aborto Habitual/imunologia , Aborto Habitual/terapia , Aborto Habitual/virologia , Acridinas/uso terapêutico , Adulto , Doença Crônica , Citomegalovirus/efeitos dos fármacos , Citomegalovirus/imunologia , Infecções por Citomegalovirus/imunologia , Infecções por Citomegalovirus/terapia , Infecções por Citomegalovirus/virologia , Feminino , Humanos , Imunoglobulinas/uso terapêutico , Imunoglobulinas Intravenosas , Fatores Imunológicos/uso terapêutico , Peptídeos/uso terapêutico , Gravidez , Complicações Infecciosas na Gravidez/imunologia , Complicações Infecciosas na Gravidez/terapia , Complicações Infecciosas na Gravidez/virologia , Primeiro Trimestre da Gravidez , Segundo Trimestre da Gravidez , Recidiva , Extratos do Timo/uso terapêutico , Replicação Viral/efeitos dos fármacos
4.
Bull Exp Biol Med ; 159(5): 623-5, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26459479

RESUMO

We studied the effects of tactivin and splenic polypeptides on learning and memory of thymectomized animals. In 3-week rats, thymectomy blocked active avoidance conditioning. Injections of tactivin (0.5 mg/kg) during 1 month after surgery restored learning capacity; splenic polypeptides were ineffective.


Assuntos
Aprendizagem da Esquiva/efeitos dos fármacos , Memória/efeitos dos fármacos , Nootrópicos/farmacologia , Peptídeos/farmacologia , Timectomia , Extratos do Timo/farmacologia , Timo/química , Animais , Aprendizagem da Esquiva/fisiologia , Condicionamento Clássico/fisiologia , Memória/fisiologia , Nootrópicos/isolamento & purificação , Especificidade de Órgãos , Peptídeos/isolamento & purificação , Ratos , Ratos Wistar , Baço/química , Extratos do Timo/isolamento & purificação , Timo/fisiologia , Timo/cirurgia
5.
Vestn Ross Akad Med Nauk ; (1): 113-7, 2015.
Artigo em Russo | MEDLINE | ID: mdl-26027280

RESUMO

OBJECTIVE: Our aim was to investigate the influence of thymic polypeptides on pain sensitivity and to analyze a possible role of the opioid system in the implementation of the analgesia caused by immobilization stress. METHODS: The study was performed on male Wistar rats at the Moscow state University named after M. V. Lomonosov. We studied effects of thymus peptides: thymuline (0.15 mg/kg), fraction 5 thymosin (0.25 microgram/kg) and cattle thymus extracted product (CTEP) (0.5 mg/kg) on pain sensitivity in rats using test "tail flick" without stress, with acute (3 h) and sub acute (12 h) immobilization stress. The comparison groups were animals treated with saline and spleen polypeptides. RESULTS: It is shown that preparations of thymus increase the threshold of pain sensitivity in the intact animals. Immobilization stress duration 3 and 12 h in thymus peptides treated rats caused a less pronounced increase in pain threshold than in the control groups (immobilization stress 3 h: CTEP--p = 0.025, thymuline--p = 0.022, fraction 5 thymosin--p = 0.033; immobilization stress 12 h: CTEP--p = 0.034, thymuline--p = 0.027, fraction 5 thymosin--p = 0.036). The opioid receptor blocker naloxone (1 mg/kg) did not completely block the stress-induced analgesia, indicating the presence of both opioid and non -opioid components in this state. In thymus peptides treated rats, opioid component was less pronounced than in the control groups (CTEP--p = 0.031, thymuline--p = 0.026, fraction 5 thymosin--p = 0.029). CONCLUSION: Pre-activation of the opioid system by the thymus polypeptides leads to an increase in the share of non-opioid component of the stress-induced analgesia and prevents the depletion of the opioid system in immobilization stress.


Assuntos
Naloxona/farmacologia , Dor , Restrição Física/efeitos adversos , Fator Tímico Circulante , Timosina , Timo/metabolismo , Analgesia/métodos , Animais , Bovinos , Masculino , Modelos Animais , Antagonistas de Entorpecentes/farmacologia , Dor/diagnóstico , Dor/tratamento farmacológico , Dor/etiologia , Dor/metabolismo , Dor/fisiopatologia , Manejo da Dor , Ratos , Ratos Wistar , Receptores Opioides/metabolismo , Fator Tímico Circulante/metabolismo , Fator Tímico Circulante/farmacologia , Timosina/metabolismo , Timosina/farmacologia , Extratos do Timo/metabolismo , Extratos do Timo/farmacologia
7.
Vestn Ross Akad Med Nauk ; (7-8): 25-9, 2014.
Artigo em Russo | MEDLINE | ID: mdl-25563001

RESUMO

AIM: The objective of this project was to explore the influence of immunoactive drugs (tactivin, thymulin, and thymosin fraction 5) on the development of the passive avoidance conditioned reflex. MATERIALS AND METHODS: Two types of passive avoidance boxes were used--a regular two-chamber box and a modified three-chamber box, comprising a dark chamber in which rats were exposed to electrical shock, a safe dark chamber, and a light chamber in the center. RESULTS: The project has established that the memory trace persists longer under the influence of the immunoactive drugs in both models, which is consistent with the reference nootropic piracetam test results. Notably, the immunoactive drugs' mnemotropic effect was more pronounced in the modified three-chamber box than in the standard two-chamber box. Using the modified box helped to establish the influence of tactivin, thymulin, and thymosin fraction 5 on the spatial memory component. Immunotropic preparations from thymus caused the animals to select the safe chamber 24 hours later and in subsequent tests. CONCLUSION: The project's results indicate that the drugs tested do possess mnemotropic properties, so their range of clinical use can be broadened.


Assuntos
Adjuvantes Imunológicos/farmacologia , Aprendizagem da Esquiva , Condicionamento Clássico , Memória , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Aprendizagem da Esquiva/fisiologia , Condicionamento Clássico/efeitos dos fármacos , Condicionamento Clássico/fisiologia , Reação de Fuga/efeitos dos fármacos , Reação de Fuga/fisiologia , Masculino , Memória/efeitos dos fármacos , Memória/fisiologia , Modelos Biológicos , Peptídeos/farmacologia , Ratos , Ratos Wistar , Fator Tímico Circulante/farmacologia , Timosina/análogos & derivados , Timosina/farmacologia , Extratos do Timo/farmacologia
10.
Bull Exp Biol Med ; 154(2): 233-6, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23330133

RESUMO

Apart from restoration of disordered immunological parameters, tactivin and derinat exhibit a pronounced effect on the higher integrative functions of the brain. Experiments on Wistar rats have shown that these drugs accelerated conditioning of food and defense responses. New methods for quantitative evaluation of memory trace consolidation are proposed.


Assuntos
Fatores Imunológicos/farmacologia , Aprendizagem/efeitos dos fármacos , Memória/efeitos dos fármacos , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/fisiologia , DNA/farmacologia , Masculino , Peptídeos/farmacologia , Ratos , Ratos Wistar , Extratos do Timo/farmacologia
11.
Eksp Klin Farmakol ; 75(11): 19-21, 2012.
Artigo em Russo | MEDLINE | ID: mdl-23323328

RESUMO

It is established in experiments on noninbred rats that the use of imunofan (20 mg/kg daily) and polyoxidonium (150 mg/kg daily) for 7 days on the background of chronic intoxication with organophosphorus agent VX (0.01 LD50, single daily treatment for 30 days) resulted in almost complete recovery of phagocytic-metabolic activity of neutrophils, the content of lysozyme, cationic protein of platelet, and levels of proinflammatory cytokines TNFa, IL-1b and IL-6 in the blood. The administration of T-activin (20 mg/kg daily for 7 days) restores these parameters insignificantly. The maximum overall stimulatory effect was produced by polyoxidonium, while the minimum effect was observed for T-activin.


Assuntos
Antídotos/farmacologia , Substâncias para a Guerra Química/toxicidade , Fatores Imunológicos/farmacologia , Oligopeptídeos/farmacologia , Compostos Organotiofosforados/toxicidade , Piperazinas/farmacologia , Polímeros/farmacologia , Animais , Antídotos/uso terapêutico , Feminino , Intoxicação por Gás/tratamento farmacológico , Intoxicação por Gás/imunologia , Fatores Imunológicos/uso terapêutico , Interleucina-1beta/sangue , Interleucina-6/sangue , Masculino , Muramidase/sangue , Neutrófilos/efeitos dos fármacos , Neutrófilos/imunologia , Oligopeptídeos/uso terapêutico , Compostos Organotiofosforados/antagonistas & inibidores , Peptídeos/farmacologia , Fagocitose/efeitos dos fármacos , Piperazinas/uso terapêutico , Polímeros/uso terapêutico , Ratos , Extratos do Timo/farmacologia , Fator de Necrose Tumoral alfa/sangue
12.
Histochem Cell Biol ; 136(1): 79-92, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21626126

RESUMO

Diabetes is a debilitating disease with chronic evolution that affects many tissues and organs over its course. Thymus is an organ that is affected early after the onset of diabetes, gradually involuting until it loses most of its thymocyte populations. We show evidence of accumulating free fatty acids with generation of eicosanoids in the diabetic thymus and we present a possible mechanism for the involution of the organ during the disease. Young rats were injected with streptozotocin and their thymuses examined for cell death by flow cytometry and TUNEL reaction. Accumulation of lipids in the diabetic thymus was investigated by histology and electron microscopy. The identity and quantitation of accumulating lipids was done with gas chromatography-mass spectrometry and liquid chromatography. The expression and dynamics of the enzymes were monitored via immunohistochemistry. Diabetes causes thymus involution by elevating the thymocyte apoptosis. Exposure of thymocytes to elevated concentration of glucose causes apoptosis. After the onset of diabetes, there is a gradual accumulation of free fatty acids in the stromal macrophages including arachidonic acid, the substrate for eicosanoids. The eicosanoids do not cause thymocyte apoptosis but administration of a cyclooxygenase inhibitor reduces the staining for ED1, a macrophage marker whose intensity correlates with phagocytic activity. Diabetes causes thymus involution that is accompanied by accumulation of free fatty acids in the thymic macrophages. Excess glucose is able to induce thymocyte apoptosis but eicosanoids are involved in the chemoattraction of macrophage to remove the dead thymocytes.


Assuntos
Ácido Araquidônico/metabolismo , Diabetes Mellitus Experimental/metabolismo , Macrófagos/metabolismo , Timo/metabolismo , Animais , Diabetes Mellitus Experimental/patologia , Imuno-Histoquímica , Macrófagos/citologia , Masculino , Ratos , Ratos Sprague-Dawley , Extratos do Timo/metabolismo , Timo/citologia , Timo/patologia
13.
Cochrane Database Syst Rev ; (2): CD003993, 2011 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-21328265

RESUMO

BACKGROUND: Purified thymus extracts (pTE) and synthetic thymic peptides (sTP) are thought to enhance the immune system of cancer patients in order to fight the growth of tumour cells and to resist infections due to immunosuppression induced by the disease and antineoplastic therapy. OBJECTIVES: To evaluate the effectiveness of pTE and sTP for the management of cancer. SEARCH STRATEGY: We searched CENTRAL (The Cochrane Library 2010, Issue 3), MEDLINE, EMBASE, AMED, BIOETHICSLINE, BIOSIS, CATLINE, CISCOM, HEALTHSTAR, HTA, SOMED and LILACS (to February 2010). SELECTION CRITERIA: Randomised trials of pTE or sTP in addition to chemotherapy or radiotherapy, or both, compared to the same regimen with placebo or no additional treatment in adult cancer patients. DATA COLLECTION AND ANALYSIS: Two authors independently extracted data from published trials. We derived odds ratios (OR) from overall survival (OS) and disease-free survival (DFS) rates, tumour response (TR) rates, and rates of adverse effects (AE) related to antineoplastic treatments. We used a random-effects model for meta-analysis. MAIN RESULTS: We identified 26 trials (2736 patients). Twenty trials investigated pTE (thymostimulin or thymosin fraction 5) and six trials investigated sTP (thymopentin or thymosin α(1)). Twenty-one trials reported results for OS, six for DFS, 14 for TR, nine for AE and 10  for safety of pTE and sTP. Addition of pTE conferred no benefit on OS (RR 1.00, 95% CI 0.79 to 1.25); DFS (RR 0.97, 95% CI 0.82 to 1.16); or TR (RR 1.07, 95% CI 0.92 to 1.25). Heterogeneity was moderate to high for all these outcomes. For thymosin α(1) the pooled RR for OS was 1.21 (95% CI 0.94 to 1.56, P = 0.14), with low heterogeneity; and 3.37 (95% CI 0.66 to 17.30, P = 0.15) for DFS, with moderate heterogeneity. The pTE reduced the risk of severe infectious complications (RR 0.54, 95% CI 0.38 to 0.78, P = 0.0008; I² = 0%). The RR for severe neutropenia in patients treated with thymostimulin was 0.55 (95% CI 0.25 to 1.23,  P = 0.15). Tolerability of pTE and sTP was good. Most of the trials had at least a moderate risk of bias. AUTHORS' CONCLUSIONS: Overall, we found neither evidence that the addition of pTE to antineoplastic treatment reduced the risk of death or disease progression nor that it improved the rate of tumour responses to antineoplastic treatment. For thymosin α(1), there was a trend for a reduced risk of dying and of improved DFS. There was preliminary evidence that pTE lowered the risk of severe infectious complications in patients undergoing chemotherapy or radiotherapy.


Assuntos
Adjuvantes Imunológicos/uso terapêutico , Sistema Imunitário/efeitos dos fármacos , Neoplasias/imunologia , Peptídeos/uso terapêutico , Extratos do Timo/uso terapêutico , Timo/química , Adjuvantes Imunológicos/efeitos adversos , Adulto , Intervalo Livre de Doença , Feminino , Humanos , Hospedeiro Imunocomprometido , Masculino , Neoplasias/tratamento farmacológico , Peptídeos/efeitos adversos , Timalfasina , Timopentina/uso terapêutico , Timosina/análogos & derivados , Timosina/uso terapêutico , Extratos do Timo/efeitos adversos
14.
J Alzheimers Dis ; 22(2): 641-52, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20858976

RESUMO

Different mechanisms including oxidative stress are proposed for amyloid-ß peptide (Aß) neurotoxicity, and here we contribute to demonstrate that nitro-oxidative stress is playing a key role. Yeasts are a well-known model for H2O2 toxicity. Interestingly, yeast cell wall prevents interaction of Aß fibrils with membrane receptors or calcium channels and we found a significant viability reduction in yeasts when challenged with Aß fibrils. Furthermore, iron and copper chelators, as well as the antioxidants glutathione and trolox, were neuroprotective on neuroblastoma cells and mouse hippocampal neurons challenged with Aß fibrils. Glutathione prevents the oxidation, glycation and nitrotyrosination of cell proteins induced by Aß. Trolox protected neurons in cell viability studies, maintaining the vesicular transport integrity and preventing the trigger of apoptotic mechanisms. Interestingly, we have also found that brain derived neuronal factor (BDNF) and neurotrophin-3 (NT-3) were able to protect mouse hippocampal and cortical neurons against H2O2 and Aß fibrils. Considering that superoxide anion, produced by Aß cell damage, and nitric oxide, whose production is altered in AD, react to form the highly reactive peroxynitrite anion, we studied the role of trolox to ameliorate the peroxynitrite cell damage. Finally, one of the major proteins to be nitrotyrosinated in AD, the triose phosphate isomerase (TPI) was assayed searching for a denitrase activity that could reverse intracellular nitrotyrosination. We have found that human neuroblastoma SH-SY5Y cells express a constitutive denitrase activity that partially denitrated nitro-TPI. Altogether, our results support a key role of nitro-oxidative stress in the neuronal damage induced by Aß fibrils.


Assuntos
Peptídeos beta-Amiloides/farmacologia , Amiloide/metabolismo , Neurônios/efeitos dos fármacos , Óxido Nítrico/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Idoso , Doença de Alzheimer/patologia , Peptídeos beta-Amiloides/metabolismo , Animais , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Sobrevivência Celular , Células Cultivadas , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Cromanos/farmacologia , Desferroxamina/farmacologia , Relação Dose-Resposta a Droga , Embrião de Mamíferos , Glutationa/metabolismo , Hipocampo/citologia , Humanos , Peróxido de Hidrogênio/farmacologia , Imunoprecipitação/métodos , Marcação In Situ das Extremidades Cortadas/métodos , Masculino , Camundongos , Modelos Biológicos , Doadores de Óxido Nítrico/farmacologia , Nitroprussiato/farmacologia , Fragmentos de Peptídeos/farmacologia , Ratos , Sideróforos/farmacologia , Extratos do Timo/metabolismo
15.
Pol J Vet Sci ; 13(2): 233-40, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20731176

RESUMO

In order to determine the effect of apoptosis and necrosis on the intensity of the muscular phase of infection by Trichinella spiralis, male CFW mice were orally infected with T. spiralis larvae and treated with some immunomodulating drugs: calf thymus extract (TFX), lipopolysaccharide from Escherichia coli (LPS), and dexametasone (DEX). Treatment with TFX increased the proportion of apoptotic lymphocytes and decreased the proportion of necrotic lymphocytes from 14 to 60 days after infection in mice infected with T. spiralis. Treatment with LPS increased proportions of both apoptotic and necrotic lymphocytes from 21 to 60 days after infection, especially at 28 days after infection. Treatment with DEX increased the proportion of apoptotic lymphocytes only at 28 days after infection, and significantly increased the proportion of necrotic lymphocytes at 21 days after infection. Parasite load in the affected muscle tissue was significantly lower than the control in mice treated with TFX, not significantly different from the control in mice treated with LPS, and significantly higher than the control in mice treated with DEX. The results of the study suggest that the parasite made an effort to reduce the effectivity of the host immune response in order to ensure its own survival.


Assuntos
Imunossupressores/farmacologia , Inflamação/metabolismo , Linfócitos/fisiologia , Músculo Esquelético/patologia , Trichinella spiralis , Triquinelose/patologia , Animais , Apoptose , Bovinos , Dexametasona/administração & dosagem , Dexametasona/farmacologia , Imunossupressores/administração & dosagem , Lipopolissacarídeos/administração & dosagem , Lipopolissacarídeos/farmacologia , Masculino , Camundongos , Músculo Esquelético/parasitologia , Necrose , Extratos do Timo/administração & dosagem , Extratos do Timo/farmacologia , Triquinelose/parasitologia
16.
BMC Cancer ; 10: 457, 2010 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-20735834

RESUMO

BACKGROUND: Thymostimulin is a thymic peptide fraction with immune-mediated cytotoxicity against hepatocellular carcinoma (HCC) in vitro and palliative efficacy in advanced HCC in two independent phase II trials. The aim of this study was to assess the efficacy of thymostimulin in a phase III trial. METHODS: The study was designed as a prospective randomised, placebo-controlled, double-blind, multicenter clinical phase III trial. Between 10/2002 and 03/2005, 135 patients with locally advanced or metastasised HCC (Karnofsky >or=60%/Child-Pugh

Assuntos
Carcinoma Hepatocelular/tratamento farmacológico , Indutores de Interferon/uso terapêutico , Neoplasias Hepáticas/tratamento farmacológico , Extratos do Timo/uso terapêutico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Hepatocelular/secundário , Progressão da Doença , Método Duplo-Cego , Feminino , Seguimentos , Humanos , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Cuidados Paliativos , Placebos , Estudos Prospectivos , Taxa de Sobrevida , Resultado do Tratamento , Adulto Jovem
17.
Vestn Ross Akad Med Nauk ; (1): 23-6, 2010.
Artigo em Russo | MEDLINE | ID: mdl-20408435

RESUMO

The action of the immunocorrective agent tactivin on CNS functional activity was studied in an experiment using outbred white rats. The drug was shown to affect the animals' behaviour in an elevated cross-shaped maze including learning capacity and ability to overcome functional disorders of conditioned active avoidance response. The data obtained suggest anti-stress activity of tactivin.


Assuntos
Adjuvantes Imunológicos/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Comportamento Animal/efeitos dos fármacos , Peptídeos/farmacologia , Extratos do Timo/farmacologia , Animais , Masculino , Ratos
18.
Folia Neuropathol ; 48(4): 246-57, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21225507

RESUMO

The aim of this study was to evaluate the efficacy of treatment of Lewis rats with calf thymus extract (TFX®) and its six-peptide fraction on the course of experimental allergic encephalomyelitis (EAE). Interferon- ß served as a reference drug. We found that intramuscular administration of the thymus extract fraction significantly reduced clinical, immunological, histological, and ultrastructural alterations inherent in the disease. We suggest that TFX® or TFX®-derived fractions have potential as therapeutics in treatment of neurodegenerative diseases such as multiple sclerosis.


Assuntos
Encefalomielite Autoimune Experimental/tratamento farmacológico , Encefalomielite Autoimune Experimental/patologia , Extratos do Timo/uso terapêutico , Animais , Bovinos , Encefalomielite Autoimune Experimental/imunologia , Feminino , Imuno-Histoquímica , Inflamação/tratamento farmacológico , Inflamação/imunologia , Inflamação/patologia , Ratos , Ratos Endogâmicos Lew , Medula Espinal/efeitos dos fármacos , Medula Espinal/imunologia , Medula Espinal/patologia
19.
Adv Gerontol ; 23(4): 543-6, 2010.
Artigo em Russo | MEDLINE | ID: mdl-21510076

RESUMO

The interference between thymus and pineal gland during their involution is considered in this review. The research data about influence of thymus peptides on pineal gland and pineal peptides on thymus is summarized. Analysis of these data showed that pineal peptides (epithalamin, epitalon) had more effective geroprotective effect on thymus involution in comparison with geroprotective effect of thymic peptides (thymalin, thymogen) on involution of pineal gland. The key mechanisms of pineal peptides effect on thymus dystrophy is immunoendocrine cooperation, which is realized as transcription's activation of various proteins.


Assuntos
Envelhecimento/metabolismo , Hormônios Peptídicos/metabolismo , Glândula Pineal/metabolismo , Timo/metabolismo , Adjuvantes Imunológicos/metabolismo , Envelhecimento/imunologia , Animais , Procedimentos Cirúrgicos Endócrinos/efeitos adversos , Terapia de Reposição Hormonal , Humanos , Melatonina/metabolismo , Melatonina/uso terapêutico , Modelos Animais , Hormônios Peptídicos/uso terapêutico , Glândula Pineal/cirurgia , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/terapia , Extratos do Timo/uso terapêutico , Timo/cirurgia
20.
BMC Complement Altern Med ; 9: 51, 2009 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-20003431

RESUMO

BACKGROUND: Congaplex and Immuplex are dietary supplements that have been traditionally used to support immune system function. The purpose of these experiments was to determine whether Congaplex and Immuplex affect immune function using primary and immortalized T lymphocytes. METHODS: Immortalized CEM and Jurkat T lymphocytes and primary peripheral mononuclear blood cells (PBMCs) were treated with the aqueous extracts from Congaplex and Immuplex to determine the effects of these products on cytokine production in activated T lymphocytes. RESULTS: Congaplex enhanced phytohemagglutinin/phorbol 12-myristate 13-acetate (PHA/PMA) stimulation of both CEM and Jurkat cells as measured by the production of cytokines, while Immuplex suppressed PHA/PMA-induced production of cytokines, with the exception of interleukin (IL)-8 which was enhanced by Immuplex. In vitro treatment of PBMCs from 10 healthy donors with Congaplex or Immuplex decreased PHA-stimulated production of interferon (IFN)-gamma but increased the production of IL-13. CONCLUSIONS: While the effects of Congaplex and Immuplex differed in these two models, these data demonstrate that the aqueous extracts from these two dietary supplements can affect the inflammatory response of T lymphocytes.


Assuntos
Citocinas/biossíntese , Suplementos Nutricionais , Fatores Imunológicos/farmacologia , Fitoterapia , Linfócitos T/efeitos dos fármacos , Linhagem Celular , Combinação de Medicamentos , Humanos , Interferon gama/biossíntese , Interleucina-13/biossíntese , Interleucina-8/biossíntese , Células Jurkat , Micronutrientes/farmacologia , Fito-Hemaglutininas/farmacologia , Extratos Vegetais/farmacologia , RNA Mensageiro/biossíntese , Linfócitos T/metabolismo , Acetato de Tetradecanoilforbol/farmacologia , Extratos do Timo/farmacologia
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