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1.
Water Sci Technol ; 81(5): 985-999, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32541116

RESUMO

Microencapsulation technology was adopted to prepare the novel mineral-based mesoporous microsphere (MBMM) for the removal of dye contaminants from water. Field emission scanning electron microscopy, energy dispersive spectrometry, Brunauer-Emmett-Teller zeta potential analysis, and Fourier transform infrared spectrometry were used to investigate the microstructure characteristics of MBMM and its changes in the functional groups before and after adsorption. Batch experiments were carried out to investigate the effect of calcination temperature, initial concentration, pH, contact temperature, and time on the adsorption behavior of rhodamine B and methylene blue onto MBMM. The results indicated that the prepared MBMM had a hollow structure and mesoporous surface, which was beneficial to improving its adsorption capacity. The maximum adsorption capacities of rhodamine B and methylene blue onto MBMM prepared at calcination temperature 500 °C were 57.79 mg g-1 and 55.94 mg g-1 under the conditions of initial concentration 300 mg L-1, dosage 0.1 g, pH 7.0, adsorption temperature 55 °C, and adsorption time 7 h. The results showed that the calcining treatment was beneficial to the formation of mesoporous microspheres, improving their adsorption capacities. The adsorption process was endothermic reaction, and electrostatic attraction and hydrogen bonding were the driving forces of the reaction.


Assuntos
Poluentes Químicos da Água , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Microesferas , Minerais , Espectroscopia de Infravermelho com Transformada de Fourier
2.
In Vivo ; 34(3 Suppl): 1637-1644, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32503823

RESUMO

BACKGROUND: Sneezes produce many pathogen-containing micro-droplets with high velocities of 4.5-50.0 m/s. Face masks are believed to protect people from infection by blocking those droplets. However, current filtration efficiency tests can't evaluate masks under sneeze-like pressure. The goal of this study was to establish a method to evaluate the filtration efficiency of mask materials under extreme conditions. MATERIALS AND METHODS: Efficiency of surgical masks, gauze masks, gauze, cotton, silk, linen and tissue paper on blocking micro-droplet sized starch particles (average 8.2 µm) and latex microspheres (0.75 µm) with a velocity of 44.4 m/s created by centrifugation was qualitatively analyzed by using imaging-based analysis. RESULTS: The 4 layers of silk could block 93.8% of microspheres and 88.9% of starch particles, followed by the gauze mask (78.5% of microspheres and 90.4% of starch particles) and the 2 layers of cotton (74.6% of microspheres and 87.5-89.0% of particles). Other materials also blocked 53.2-66.5% of microspheres and 76.4%-87.9% of particles except the 8 layers of gauze which only blocked 36.7% of particles. The filtration efficiency was improved by the increased layers of materials. CONCLUSION: Centrifugation-based filtration efficiency test not only compensates shortcomings of current tests for masks, but also offers a simple way to explore new mask materials during pandemics. Common mask materials can potentially provide protection against respiratory droplet transmission.


Assuntos
Centrifugação/métodos , Controle de Infecções/instrumentação , Máscaras , Teste de Materiais/métodos , Espirro , Filtração , Humanos , Interações Hidrofóbicas e Hidrofílicas , Microesferas , Papel , Tamanho da Partícula , Material Particulado , Pressão , Eletricidade Estática , Têxteis
4.
Artigo em Inglês | MEDLINE | ID: mdl-32361632

RESUMO

Edoxaban is mainly enzymatically converted to a 4-carboxylic acid form (4CA-EDX) and an N-desmethyl form (ND-EDX) in humans. This study aimed to establish a simple liquid chromatography-tandem mass spectrometry method using core-shell octadecyl silica (ODS) microparticles for the simultaneous quantitation of edoxaban and its two major metabolites in human plasma. Analytes extracted from plasma specimens by a one-step deproteinization were separated using a 2.6-µm core-shell ODS microparticulate column and linear acetonitrile-ammonium acetate gradient elution at a flow rate of 0.25 mL/min with a run time of 7 min. The mass spectrometer was operated in the positive ion multiple reaction monitoring mode. Plasma samples collected from 20 patients with atrial fibrillation were analyzed by the present method. The chromatograms of drug-free human plasma had no interfering peaks. The calibration curves of edoxaban, 4CA-EDX, and ND-EDX were linear over the concentration ranges of 1.25-160, 0.47-60, and 0.12-15 ng/mL, respectively. Their pretreatment recoveries and matrix factors were 88.7-109.0% and 87.0-101.6%, respectively. The intra- and inter-day accuracy and imprecision values were 85.9-112.8% and within 13.3%, respectively. The plasma concentrations of edoxaban, 4CA-EDX, and ND-EDX in the patients had ranges of 17.8-102, 1.67-25.7, and 0.685-5.34 ng/mL, respectively. All the analytes were measurable within their calibration curves. In conclusion, this validated method for the simultaneous determination of edoxaban and its major metabolites was successfully applied to plasma specimens obtained from patients with atrial fibrillation.


Assuntos
Piridinas/sangue , Piridinas/farmacocinética , Espectrometria de Massas em Tandem/métodos , Tiazóis/sangue , Tiazóis/farmacocinética , Técnicas Biossensoriais , Coleta de Amostras Sanguíneas , Cromatografia Líquida de Alta Pressão , Humanos , Limite de Detecção , Metabolômica , Microesferas , Padrões de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Dióxido de Silício/química
5.
Aquat Toxicol ; 224: 105497, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32388340

RESUMO

Previous research has identified microplastics as new environmental pollutants that are widely distributed in a variety of environments, including aquaculture environments. However, the potential hazard of microplastics to aquaculture animals, especially toward lipid metabolism involved with the survival and growth of aquatic animal, has not yet been investigated. In the present study, redclaw crayfish (Cherax quadricarinatus) were exposed to different concentrations of 200 nm-sized polystyrene microspheres (0, 0.5, and 5 mg/L) for 21 days, to investigate the effects of microplastics on lipid metabolism. After ingestion, the microplastics were distributed in the intestines and hepatopancreas, and appeared to inhibit the growth of Cherax quadricarinatus. Subsequently, the lipid levels in the hepatopancreas and hemolymph was detected, and found that after 21 days of exposure, the lipid content and free fatty acids in the hepatopancreas and hemolymph decreased significantly, and total cholesterol and triglycerides levels increased significantly in the hemolymph. This might have been caused by insufficient intake of exogenous fat. A significant decrease in lipase activity also supported this view. The activity of lipoprotein lipase related to lipolysis in the hepatopancreas increased significantly, while the activity of fatty acid synthase related to fat synthesis increased, and the activity of acetyl-CoA carboxylase decreased. These results indicated disturbed lipid metabolism in the hepatopancreas. The significant increase of lipid transport-related low-density lipoprotein indicated that the lipolytic capacity was higher than the lipid synthesis capacity. The expression levels of fatty acid metabolism-related genes FAD6 and FABP decreased significantly, indicating that the fatty acid utilization ability of hepatopancreas cells was inhibited, which was consistent with the results of enzyme activities. Thus, microplastics represent a potential hazard to redclaw crayfish, at least on lipid metabolism. This study provided basic data on the ecotoxicological effects of microplastics on crustaceans.


Assuntos
Astacoidea/efeitos dos fármacos , Hepatopâncreas/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Poliestirenos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Aquicultura , Astacoidea/metabolismo , Hemolinfa/efeitos dos fármacos , Hemolinfa/metabolismo , Hepatopâncreas/metabolismo , Microesferas , Poliestirenos/química , Poliestirenos/metabolismo , Poluentes Químicos da Água/química , Poluentes Químicos da Água/metabolismo
6.
Adv Clin Exp Med ; 29(4): 431-440, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32364686

RESUMO

BACKGROUND: Autogenous or allogenic bone transplantation is the main treatment for bone defects and nonunions. However, the shortcomings of autogenous or allogenic bone transplantation limit its wide application in clinical use. OBJECTIVES: This study investigated the effect of poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with pOsterix (pOsx)/polyethylenimine (PEI) nanoparticles in repairing bone defects and explored its mechanism. MATERIAL AND METHODS: Poly(lactic-co-glycolic acid) microspheres loaded with pOsx/PEI nanoparticles were constructed. The Osx transfection effect was detected by fluorescence quantitative PCR and western blotting methods. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) and flow cytometry methods were used to detect cell proliferation. The collagen I (Col-1), osteopontin (OPN) and osteocalcin (OC) expression levels were detected using real-time polymerase chain reaction (RT-PCR) and western blotting methods. Bone defect model was constructed. Bone repair was detected using X-ray, hematoxylin and eosin (H&E) staining, and Mason staining methods. RESULTS: PLGA@pOsx/PEI has transfection effect both in vitro and in vivo, does not affect cell proliferation and is safe for cells. PLGA@pOsx/PEI could promote the expression of Col-1, OPN and OC in vitro and in vivo. PLGA@pOsx/PEI could promote osteogenesis in vivo. CONCLUSIONS: PLGA@pOsx/PEI with high Osx expression could promote the expression of OC, OPN, and COL-I. PLGA@pOsx/PEI can be used as a material for repairing bone defects and can promote bone formation. These results provide a theoretical and practical basis for its further clinical application.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Ácido Láctico/química , Nanopartículas , Polietilenoimina/química , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Western Blotting , Proliferação de Células/efeitos dos fármacos , Microesferas , Reação em Cadeia da Polimerase em Tempo Real
7.
Waste Manag ; 108: 119-126, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32353776

RESUMO

Catalytic conversion of xylose and the hemicellulose fraction of waste biomass to furfural is important for the valorization of waste lignocellulose. Here, a clean and efficient catalytic system consisting of sulfonated carbon microspheres catalysts and γ-valerolactone was developed for the upgrading of xylose and waste lignocellulose to the furfural in one-pot. Sulfonated carbon microspheres (CCoS) with Brønsted and Lewis acid sites were prepared to yield furfural. The mesoporous structures were facilitated by introduction of Co element in xylose hydrothermal process, and the density of Brønsted acid sites were improved by the sulfonation. The furfural yield from xylose reached 75.12% using CCoS as catalyst at 170 °C for 30 min in a γ-valerolactone/water (17/3 v/v) solvent. As typical Brønsted acid, the SO3H groups on the surface of CCoS catalyst is essential for catalytic dehydration xylose to furfural. Additionally, the mesoporous structures of CCoS improved the mass transfer in the furfural production process. The catalytic system was applied in the conversion of real biomass (including corncob, corn straw and Eucalyptus sawdust) to evaluate the possibility of application. These three biomass species all reached excellent furfural yields, which were more than 70%. This work provided a catalytic strategy for effective conversion of xylose and biomass to furfural.


Assuntos
Carbono , Furaldeído , Catálise , Lignina , Microesferas , Xilose
8.
Mar Pollut Bull ; 156: 111208, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32366368

RESUMO

Jellyfish are voracious planktonic predators that may be susceptible to ingesting microplastics. We measured rates of ingestion and egestion of microbeads by Aurelia aurita (Scyphozoa) and evaluated whether ingesting microbeads affected metabolism or gut epithelia. Ingestion rates were measured by exposing medusae to microbeads and randomly sampling them 6 times over a 32 h period to determine the number of microbeads in their tissues. Egestion rates were measured by exposing medusae to microbeads for 1 h before transferring them to kreisels without microbeads and sampling them 6 times over 8 h. Respiration rates of medusae were determined using incubations and potential damage to gut epithelia was evaluated using histopathology. Medusae ingested few microbeads and egested them within 8 h. Microbeads had no effect on respiration and the histology. We concluded that the medusae may recognise microbeads as non-food particles and that their ingestion caused undetectable physiological and histological harm.


Assuntos
Plásticos , Cifozoários , Animais , Ingestão de Alimentos , Microesferas
9.
J Biomed Nanotechnol ; 16(2): 153-165, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32252877

RESUMO

Although the percutaneous coronary intervention (PCI)treatment can improve the survival rate of acute myocardial infarction (AMI) patients, the early granulocytes response within 6 hours can induce second injuries during the reperfusion process. The new drug delivery system MMP9 hydrolytic microspheres (NMM) with negatively charged surface was designed out and MCC950 (MCC) was loaded into NMM (NMM-M), MCC is the inhibitor of nucleotide binding oligomerization domain (NOD)-like receptor, pyrin containing domain 3 (NLRP3)-inflammasome which is the key promoter of granulocytes-induced injury. NMM-M could effectively escape the phagocytosis of immune phagocytes in the blood, and target the ischemic region based on the electrostatic attraction and the attraction of enzyme to substrate, and sudden release the loaded MCC within 2 hours. The released MCC can inhibit the NLRP3-inflammasome activity, and then further inhibit the secretion of inflammatory factors in granulocytes which are the main factors of early inflammatory damage, and improving cardiac function, realizing the goal of pre-treatment. Therefore, NMM may be a new delivery system, which can provide the accurately, sufficient and rapidly drug deliver, and MCC may be a novel candidate drug in AMI treatment, which may be hopeful in the future.


Assuntos
Infarto do Miocárdio , Humanos , Inflamassomos , Microesferas , Proteína 3 que Contém Domínio de Pirina da Família NLR , Intervenção Coronária Percutânea
10.
Environ Sci Pollut Res Int ; 27(18): 22631-22638, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32319059

RESUMO

A simple colloidal crystal template method was used to prepare Sn/TiO2/graphite carbon microsphere composites (xSn/TiO2/GCM, x = 2.0, 1.0, 0.2, 0.5) with porous layers. Then, the composites were represented using X-ray diffraction, energy dispersive spectrometry, scanning electron microscopy, transmission electron microscopy, and nitrogen physical adsorption/desorption. Meanwhile, the photocatalytic activities in CO2 reduction were studied under simulation of visible light exposure. It was confirmed that the Sn/TiO2/GCM composites had layered porosity, graphitized carbon matrix, and high metal compound content, and their morphology was greatly affected by the acetone amount. The outputs of CO and CH4 coming into the photocatalytic CO2 reduction reaction of Sn/TiO2/GCM were 619.46 and 14.46 µmol g-1, respectively. Among the two products, the highest production rate observed in 0.5Sn/TiO2/GCM. Because of these factors, the layered porous Sn/TiO2/GCM composites have good photocatalytic performance under simulated visible light irradiation and have unique composition and structure characteristics, which give broad application prospects in electrode materials, catalysts, and adsorbents. Graphical abstract.


Assuntos
Grafite , Carbono , Dióxido de Carbono , Catálise , Microesferas , Porosidade , Titânio
11.
Nat Biomed Eng ; 4(5): 518-530, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32313101

RESUMO

The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging. Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices. The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element. Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition. It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.


Assuntos
Bioensaio/métodos , Corantes Fluorescentes/química , Nanopartículas/química , Animais , Células da Medula Óssea/citologia , Linhagem Celular Tumoral , Coloides/química , Células Dendríticas/citologia , Feminino , Citometria de Fluxo , Fluorescência , Humanos , Imunoensaio , Lipopolissacarídeos/farmacologia , Camundongos Endogâmicos C57BL , Microesferas , Proteômica , Padrões de Referência
12.
Artigo em Inglês | MEDLINE | ID: mdl-32172172

RESUMO

Purification of small bioactive peptides from complex biological samples is a difficult task due to the interference of concentrated large biomolecules. In this study, a magnetic immobilized metal affinity chromatography matrix modified by poly (ethylene glycol) methyl ether (IMACM@mPEG) was prepared and applied for the rapid purification of angiotensin I-converting enzyme (ACE) inhibitory peptides from casein hydrolysate. The proposed IMACM@mPEG considerably reduced the non-specific adsorption of large proteins and exhibited improved purification efficiency towards ACE inhibitory peptides. A novel peptide with moderate ACE inhibitory activity (IC50 value of 274 ± 5 µM) was identified as LLYQEPVLGPVR. Lineweaver-Burk plot confirmed the non-competitive inhibition pattern of LLYQEPVLGPVR. The purified peptide was digested after simulated gastrointestinal digestion and produced shorter peptides which contributed to enhanced ACE inhibitory activity. These results indicated that the IMACM@mPEG is an effective method for the prepurification of ACE inhibitory peptide and the purified peptide LLYQEPVLGPVR may have potential as nutraceutical ingredient in functional foods for hypertension treatments.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/isolamento & purificação , Caseínas/química , Cromatografia de Afinidade/métodos , Éteres/química , Peptídeos/isolamento & purificação , Polietilenoglicóis/química , Adsorção , Sequência de Aminoácidos , Inibidores da Enzima Conversora de Angiotensina/análise , Inibidores da Enzima Conversora de Angiotensina/metabolismo , Cobre/química , Óxido Ferroso-Férrico/química , Microesferas , Peptídeos/análise , Peptídeos/metabolismo , Hidrolisados de Proteína , Dióxido de Silício/metabolismo , Propriedades de Superfície
13.
Arch Virol ; 165(5): 1049-1056, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32144545

RESUMO

Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is the predominant cause of severe enteropathogenic diarrhea in swine. A simple, rapid, specific, and sensitive method is critical for monitoring PEDV on pig farms. In this study, a simple and rapid lateral flow immunoassay detection system that integrates europium (Eu) (III) chelate microparticles was developed to identify PEDV in fecal swabs. This newly developed diagnostic sandwich immunoassay utilizes lateral flow test strips (LFTSs). The fluorescence peak heights of the test line (HT) and the control line (HC) were measured using a fluorescence strip reader, and the HT/HC ratio was used for quantitation. The limit of detection of PEDV with this LFTS was ??ten times the median tissue culture infectious dose (TCID50) per mL??. Fecal swab samples were used to determine the cutoff value. Field samples, various PEDV strains and other viruses were used to determine the sensitivity and specificity of the Eu (III) chelate microparticle-based LFTSs, which were 97.8% and 100%, respectively, with a cutoff value of 0.05, as compared with reverse transcription polymerase chain reaction (RT-PCR). In samples from piglets experimentally infected with PEDV, the results were in high agreement with those obtained by RT-PCR. Epidemiological surveillance of PEDV using the LFTSs ??in areas threatened by African swine fever virus?? suggested that the PEDV positive rate on pig farms had significantly decreased, mainly due to the implementation of strict biosecurity measures. The results indicate that the Eu (III) chelate microparticle-based LFTS system is a rapid, sensitive, and reliable method for the identification of PEDV, indicating its suitability for epidemiological surveillance of PEDV infection.


Assuntos
Infecções por Coronavirus/veterinária , Testes Diagnósticos de Rotina/métodos , Diarreia/veterinária , Imunoensaio/métodos , Compostos Organometálicos , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Doenças dos Suínos/diagnóstico , Animais , Infecções por Coronavirus/diagnóstico , Diarreia/diagnóstico , Fezes/virologia , Microesferas , Compostos Organometálicos/metabolismo , Sensibilidade e Especificidade , Suínos , Fatores de Tempo
14.
J Chromatogr A ; 1620: 461013, 2020 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-32201037

RESUMO

Dummy molecularly imprinted microspheres (DMIMs) were synthesized by Pickering emulsion polymerization and used as the matrix solid-phase dispersion extraction (MSPD) sorbent for sample pre-treatment of azole fungicides in fish samples. Alpha-(2,4-Dichlorophenyl)-1H-imidazole-1-ethanol (DCE) was used as the fragment dummy template for the imprinting of climbazole (CBZ), clotrimazole (CMZ) and miconazole (MNZ). The morphology of the microspheres were characterized by scanning electron microscopy (SEM) and nitrogen adsorption measurements, narrow diameter distribution (20-50 µm) with regular spherical shape and high surface area (SBET = 408.91 ± 6.72 m2 g-1) were achieved. Good class-selectivity of the DMIMs was found for CBZ, CMZ and MNZ by static adsorption experiments. The imprinted microspheres as MSPD sorbent was then evaluated for the extraction and purification of CBZ, CMZ and MNZ in fish samples. The extracted azole fungicides were detected by HPLC-DAD analysis at 225 nm. MSPD conditions including elution, mass ratio of sample/sorbent and washing were carefully evaluated. The optimized MSPD method have good recoveries (89.2-101.5%) and reproducibility (RSDs 1.6-4.8%, n = 5) for fish samples spiked at 0.5, 2.5 and 12.5 µg g-1. The limits of detection (LODs) were 0.045, 0.036 and 0.033 µg g-1 for CBZ, CMZ and MNZ, respectively. The results show that this method has a good application prospect for the pretreatment of azole fungicides in fish samples.


Assuntos
Azóis/isolamento & purificação , Emulsões/química , Peixes/metabolismo , Fungicidas Industriais/isolamento & purificação , Microesferas , Impressão Molecular/métodos , Polimerização , Extração em Fase Sólida/métodos , Adsorção , Animais , Limite de Detecção , Tamanho da Partícula , Porosidade , Reprodutibilidade dos Testes , Soluções , Temperatura
15.
J Environ Sci (China) ; 91: 92-104, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32172986

RESUMO

Highly efficient and sustainable conversion technologies to generate uniform sodalite (Na8(AlSiO4)6(OH)2) zeolite microspheres with low-grade waste natural diatomite as raw materials via a solution-mediated crystallization route were developed in the present study. The synthesis process can be considered as an in-situ zeolitization of diatomite precursor without involving any mesoscale template and any post-synthetic modification. The mass ratios of diatomite and AlCl3·6H2O have remarkable effect on the morphology, crystal structure and porosity of sodalite zeolite product. The preferred sodalite microspheres with uniform mesoporous of size 3.5-5.5 nm and large surface area of 162.5 m2/g exhibit well removal performance for heavy metal ions (Pb(II), Cd(II), Zn(II), and Cu(II)), with the highest adsorption abilities for Pb(II) ions of 365 mg/g. In addition, the effect of contact time, initial ion concentration, competitive adsorption and solution pH were evaluated. The removal performance results from synergistic effects of dominating cation-exchange and additional surface chemisorption. The study may broadly help unveil chemical control reactions of the zeolitization processes of diatomite, and thus facilitates the development of promising zeolite materials for the use in natural and engineered aquatic environments by recycling waste diatomite resources.


Assuntos
Metais Pesados , Poluentes Químicos da Água/análise , Zeolitas , Adsorção , Terra de Diatomáceas , Concentração de Íons de Hidrogênio , Microesferas
16.
Food Chem ; 317: 126377, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32113137

RESUMO

Ligusticum chuanxiong extract-polylactic acid sustained-release microspheres (LCE-PLA) are fabricated in this study for enhancing both duration and hepatoprotective efficacy of the main bioactive ingredients. LCE-PLA in vitro release, cytotoxicity and in vivo hepatoprotective effect were discussed to evaluate its efficiency and functionality. Results demonstrated that the optimal drug-loading rate and encapsulation efficiency of tetramethylpyrazine (TMP, the main active ingredient) were 8.19%, 83.72%, respectively. The LCE-PLA in vitro release of TMP showed prolong 5-fold and in vitro cytotoxicity declined 25.00% compared with naked LCE. After 6 weeks of in vivo intervention in high fat diet mice, both liver aspartate aminotransferase and alanine aminotransferase levels were higher in LCE-PLA group than LCE group. The above results indicated that TMP had a higher bioavailability of hepatoprotection when encapsulation of LCE-PLA was applied. The current study has provided a promising novel way to enhance the efficacy of short half-life ingredients.


Assuntos
Ligusticum/química , Extratos Vegetais/química , Poliésteres/química , Pirazinas/administração & dosagem , Pirazinas/farmacocinética , Animais , Preparações de Ação Retardada , Meia-Vida , Células Hep G2 , Humanos , Fígado/efeitos dos fármacos , Masculino , Camundongos , Microesferas
17.
AJR Am J Roentgenol ; 214(5): 1158-1164, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32130046

RESUMO

OBJECTIVE. The aim of this study was to evaluate the amount of free radioactivity in renal and intestinal excretions during the first 48 hours after transarterial radioembolization (TARE) procedures on the liver. SUBJECTS AND METHODS. Urinary, intestinal, and biliary excretions of patients who underwent TARE with three different types of microspheres were collected during a postinterventional period of 48 hours (divided into two 24-hour intervals). Radioactivity measurements were performed. The detected amounts of activity were correlated to clinical and procedural characteristics, times of excretion, and microsphere types. RESULTS. Twenty-four patients were evaluated, 10 treated with 90Y-glass, 10 with 90Y-resin, and four with 166Ho-poly-L-lactic acid (PLLA) microspheres. Activity excretion occurred in all cases. The highest total excretion proportions of the injected activities were 0.011% for 90Y-glass, 0.119% for 90Y-resin, and 0.005% for 166Ho-PLLA microspheres. Intestinal excretion was markedly less than renal excretion (p < 0.001). Excretion after TARE with 90Y-resin was statistically significantly higher than with 90Y-glass or 166Ho-PLLA micro-spheres (p = 0.002). For each microsphere type, the excreted activity was independent of the activity of the injected microspheres. CONCLUSION. Renal and intestinal excretion of radioactivity after TARE is low but not negligible. The radiation risk for individuals interacting with patients can be minimized if contact with urine and bile is avoided, particularly during the first 24 hours after the procedure.


Assuntos
Quimioembolização Terapêutica/métodos , Hólmio/farmacocinética , Eliminação Intestinal , Neoplasias Hepáticas/radioterapia , Radioisótopos/farmacocinética , Radioisótopos de Ítrio/farmacocinética , Idoso , Feminino , Hólmio/urina , Humanos , Masculino , Microesferas , Pessoa de Meia-Idade , Radioisótopos/urina , Dosagem Radioterapêutica , Radioisótopos de Ítrio/urina
18.
Medicine (Baltimore) ; 99(12): e19276, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32195932

RESUMO

This study aimed to investigate the efficacy and safety of drug-eluting beads (DEB) transarterial chemoembolization (TACE) treatment in Chinese intrahepatic cholangiocarcinoma (ICC) patients.37 ICC patients underwent DEB-TACE treatment in CTILC study (registered on clinicaltrials.gov with registry No. NCT03317483) were included in this present study. Treatment response was assessed according to modified Response Evaluation Criteria in Solid Tumors (mRECIST). Overall survival (OS) was calculated from the time of DEB-TACE operation until the date of death from any causes. Liver function change and adverse events (AEs) were recorded during and after DEB-TACE operation.3 (8.1%) patients achieved complete response (CR) and 22 (59.5%) patients achieved partial response (PR), with objective response rate (ORR) of 67.6%. After DEB-TACE treatment, mean OS was 376 days (95%CI: 341-412 days). Multivariate logistic regression analysis revealed that Bilobar disease (P = .040, OR: 0.105, 95% CI: 0.012-0.898) and portal vein invasion (P = .038, OR: 0.104, 95% CI: 0.012-0.881) could independently predict less possibility of ORR. Patients with ALB abnormal, TP abnormal, ALT abnormal and AST abnormal were increased at 1-week post DEB-TACE treatment (P = .034, P = .001, P < .001, P = .006, respectively), while returned to the levels at baseline after 1 to 3 months (all P > .050). Besides, most of the AEs were mild including pain, fever, vomiting, and nausea in this study.DEB-TACE was effective and well tolerated in treating ICC patients, and bilobar disease as well as portal vein invasion were independently correlated with less probability of ORR achievement.


Assuntos
Neoplasias dos Ductos Biliares/terapia , Quimioembolização Terapêutica/métodos , Colangiocarcinoma/terapia , Idoso , Neoplasias dos Ductos Biliares/patologia , Colangiocarcinoma/patologia , Doxorrubicina , Sistemas de Liberação de Medicamentos , Feminino , Humanos , Neoplasias Hepáticas/secundário , Modelos Logísticos , Masculino , Microesferas , Pessoa de Meia-Idade , Invasividade Neoplásica , Veia Porta/patologia
19.
Food Chem ; 319: 126539, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32193060

RESUMO

Two molecularly imprinted microspheres and two fluorescent tracers for benzimidazoles and pyrethroids were synthesized respectively. The two types of microspheres were coated in the wells of conventional microplate simultaneously. Then the sample extracts and the two traces were added for differential competition. The fluorescence intensities at two different emission wavelengths were excited and recorded for quantification of the two classes of drugs respectively. The optimized multiplexed fluorescence method could be used to determine 8 benzimidazoles and 10 pyrethroids in mutton and beef samples simultaneously. The limits of detection of the method for the 18 drugs were in the range of 5.2-17 ng/mL, and the recoveries from the standards fortified blank samples were in the range of 67.7%-109%. From the analysis of 60 real mutton and beef samples, this method could be used for multi-screening the residues of benzimidazoles and pyrethroids in meat samples.


Assuntos
Benzimidazóis/análise , Piretrinas/análise , Carne Vermelha/análise , Animais , Benzimidazóis/química , Fluorescência , Microesferas , Impressão Molecular , Estrutura Molecular , Piretrinas/química
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