Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 426
Filtrar
1.
Sci Rep ; 10(1): 1319, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31992728

RESUMO

Natural products (NP) are a valuable drug resource. However, NP-inspired drug leads are declining, among other reasons due to high re-discovery rates. We developed a conceptual framework using the metabolic fingerprint of entire ecosystems (MeE) to facilitate the discovery of global bioactivity hotspots. We assessed the MeE of 305 sites of diverse aquatic ecosystems, worldwide. All samples were tested for antiviral effects against the human immunodeficiency virus (HIV), followed by a comprehensive screening for cell-modulatory activity by High-Content Screening (HCS). We discovered a very strong HIV-1 inhibition mainly in samples taken from fjords with a strong terrestrial input. Multivariate data integration demonstrated an association of a set of polyphenols with specific biological alterations (endoplasmic reticulum, lysosomes, and NFkB) caused by these samples. Moreover, we found strong HIV-1 inhibition in one unrelated oceanic sample closely matching to HIV-1-inhibitory drugs on a cytological and a chemical level. Taken together, we demonstrate that even without physical purification, a sophisticated strategy of differential filtering, correlation analysis, and multivariate statistics can be employed to guide chemical analysis, to improve de-replication, and to identify ecosystems with promising characteristics as sources for NP discovery.


Assuntos
Produtos Biológicos , Avaliação Pré-Clínica de Medicamentos , Ecossistema , Metabolômica , Antivirais/química , Antivirais/farmacologia , Produtos Biológicos/química , Produtos Biológicos/metabolismo , Produtos Biológicos/farmacologia , Química Analítica , Análise por Conglomerados , Avaliação Pré-Clínica de Medicamentos/métodos , Geografia , Ensaios de Triagem em Larga Escala , Metabolômica/métodos
2.
Crit Rev Anal Chem ; 49(6): 553-569, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31056926

RESUMO

The increasing demand of the chiral compounds in drug and food industries has stimulated the considerable attention towards the chiral recognition and separation methods. For this, a number of chiral selectors have been employed for the recognition of enantiomers. In this context, chiral ionic liquids (CILs) have appeared as cheap and lucrative chiral selectors. The present report enlightens the role of CILs in chiral recognition of the enantiomers using various analytical techniques such as NMR and fluorescence spectroscopy, liquid and gas chromatography and capillary electrophoresis. Due to plethora of reports published in this direction, we have tried to limit our discussion about the literature published in the last decade only.


Assuntos
Química Analítica , Líquidos Iônicos/análise , Cromatografia/métodos , Eletroforese Capilar/métodos , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Fluorescência/métodos
3.
Annu Rev Biochem ; 88: 25-33, 2019 06 20.
Artigo em Inglês | MEDLINE | ID: mdl-30986087

RESUMO

Over the past six decades, steadily increasing progress in the application of the principles and techniques of the physical sciences to the study of biological systems has led to remarkable insights into the molecular basis of life. Of particular significance has been the way in which the determination of the structures and dynamical properties of proteins and nucleic acids has so often led directly to a profound understanding of the nature and mechanism of their functional roles. The increasing number and power of experimental and theoretical techniques that can be applied successfully to living systems is now ushering in a new era of structural biology that is leading to fundamentally new information about the maintenance of health, the origins of disease, and the development of effective strategies for therapeutic intervention. This article provides a brief overview of some of the most powerful biophysical methods in use today, along with references that provide more detailed information about recent applications of each of them. In addition, this article acts as an introduction to four authoritative reviews in this volume. The first shows the ways that a multiplicity of biophysical methods can be combined with computational techniques to define the architectures of complex biological systems, such as those involving weak interactions within ensembles of molecular components. The second illustrates one aspect of this general approach by describing how recent advances in mass spectrometry, particularly in combination with other techniques, can generate fundamentally new insights into the properties of membrane proteins and their functional interactions with lipid molecules. The third reviewdemonstrates the increasing power of rapidly evolving diffraction techniques, employing the very short bursts of X-rays of extremely high intensity that are now accessible as a result of the construction of free-electron lasers, in particular to carry out time-resolved studies of biochemical reactions. The fourth describes in detail the application of such approaches to probe the mechanism of the light-induced changes associated with bacteriorhodopsin's ability to convert light energy into chemical energy.


Assuntos
Microscopia Crioeletrônica/métodos , Cristalografia por Raios X/métodos , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos , Biologia Molecular/métodos , Química Analítica/história , Microscopia Crioeletrônica/história , Microscopia Crioeletrônica/instrumentação , Cristalografia por Raios X/história , Cristalografia por Raios X/instrumentação , História do Século XX , História do Século XXI , Humanos , Lasers/história , Espectroscopia de Ressonância Magnética/história , Espectroscopia de Ressonância Magnética/instrumentação , Espectrometria de Massas/história , Espectrometria de Massas/instrumentação , Biologia Molecular/história , Biologia Molecular/instrumentação , Ácidos Nucleicos/química , Ácidos Nucleicos/ultraestrutura , Proteínas/química , Proteínas/ultraestrutura
4.
Bioanalysis ; 11(7): 657-658, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997811

RESUMO

Biography MR Borenstein is Senior Associate Dean of Operations for Temple University School of Pharmacy, Associate Professor of Medicinal Chemistry, Director of the Office of Continuing Professional Education and Director of the Admissions Committee for the PharmD program. He formally served as Chair of the Department of Pharmaceutical Sciences. He is a registered pharmacist licensed to practice in Pennsylvania. His teaching responsibilities include biochemistry and medicinal chemistry. At the graduate level, he developed and taught pharmaceutical analysis and practical chromatography. His area of expertise and research involves the development of analytical techniques to investigate drug disposition in various biologic matrices and novel support materials for chromatography. He received a BA in liberal arts, a BS in pharmacy and a PhD in medicinal chemistry all from Temple University. He completed postdoctoral studies in neuropharmacology and neurochemistry at Hahnemann School of Medicine (now Drexel University) and at Temple University's Department of Chemistry.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Mobilidade Ocupacional , Humanos , Pesquisadores/psicologia
5.
Bioanalysis ; 11(7): 661-662, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997812

RESUMO

Stephanie Cape, is the Director of Bioanalytical Science at Covance, Madison, WI, USA. She received her BS in Chemistry from University of IL- Urbana/Champaign in 2003 and her PhD in Analytical Chemistry from the University of Wisconsin - Madison in 2007. Dr Cape's graduate research was focused on developing high-resolution mass spectrometric methods to enable discovery, characterization, imaging and quantitation of neuropeptides. Stephanie joined the field of regulated bioanalysis as a method development/validation chemist initially at PPD and subsequently at Covance. Within the Covance Bioanalytical team, she has held a variety of leadership roles including oversight of bioanalytical study direction, validation and method development. Additionally, Dr Cape is an active member of the broader scientific community through involvement in multiple industry groups focused on global best practices and innovation in the field of bioanalysis.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Mobilidade Ocupacional , Humanos , Indústrias , Pesquisa Interdisciplinar
6.
Bioanalysis ; 11(7): 667-669, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997814

RESUMO

Biography Shefali R Patel is a Senior Scientist and a Group leader in the Bioanalytical Group within DMPK Department at Janssen (a Johnson & Johnson company). In her current role, she is supervising the nonregulated bioanalysis group within East Coast Bioanalytical Department. Also, as a part of her current role, she is applying microsampling techniques for rodent studies within Johnson & Johnson. She has 17 years of experience in Bioanalytical Sciences. Before joining Janssen in 2007, Shefali was a Research Chemist with Drug Metabolism Department for Merck Research Laboratories in Rahway, NJ, USA. Prior to Merck, she was employed as Scientist with Taylor Technology (CRO) in Princeton, NJ, USA.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Diversidade Cultural , Indústria Farmacêutica , Humanos , Mulheres/psicologia , Local de Trabalho
7.
Bioanalysis ; 11(7): 677-678, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997815

RESUMO

Biography Nikunj Tanna is a senior scientist in the Scientific Operations department at Waters Corporation, focusing on quantification of small and large molecules for pharmaceutical, biopharmaceutical and clinical diagnostics applications. He previously led the clinical diagnostics team at Berg LLC, a Framingham, MA, USA-based biotech and clinical stage company. He feeds his curiosity with nuanced conversations with people from all walks of life. He is an avid Chelsea FC fan and loves to cook and travel. He is a proud rider of the Pan-Mass Challenge, which is a cross state bicycle ride to raise funds to support cancer research at the Dana Farber Cancer Institute in Boston, MA, USA.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Mobilidade Ocupacional , Empatia , Humanos , Poder Psicológico
8.
Bioanalysis ; 11(7): 659-660, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997817

RESUMO

Biography Chad received his Bachelor of Science degree in Chemistry from Alma College in Michigan and a Master's degree in Analytical Chemistry from the University of Michigan with a focus in the use of LC-MS/MS in protein and peptide analysis. Chad's Doctorate of Philosophy at the University of Nebraska was focused on studies of protein binding via affinity LC-MS/MS and computer simulations. Chad's career of nearly 25 years includes positions of increasing responsibility ranging from bench scientist to his current role as global head of scientific affairs for Bioanalytical Science at PRA. He is a frequently invited speaker at global scientific meetings in the field of Bioanalytical Laboratory Science including WRIB, AAPS, CPSA and Land O'Lakes Bioanalysis. He is on the organizational committee and has chaired several meetings as well including the Land O'Lakes Bioanalytical Meeting and the CPSA Meeting and the upcoming AAPS meeting on cellular and gene therapy. He is also a Bioanalysis Zone leader.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Mobilidade Ocupacional , Humanos , Indústrias , Pesquisadores/psicologia
9.
Bioanalysis ; 11(7): 675-676, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997820

RESUMO

Biography Having studied for a PhD and postdoctoral fellowship in proteomics Scott moved into the field of regulated Bioanalysis in 1997 when joining SmithKline Beecham. In 2001, Scott moved to Neuroscience Drug Discovery to lead a bioanalytical team supporting PK, in vitro DMPK and metabolite id work. In 2009, he returned to the regulated bioanalytical group, initially as a Section Leader and subsequently as Site Head and currently as WW Head of Bioanalysis at GSK. Scott has experience of small and molecule bioanalysis as well as leading both bioanalytical and discovery and development project teams across GSK.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Mobilidade Ocupacional , Comércio , Tomada de Decisões , Humanos
10.
Bioanalysis ; 11(7): 663-664, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997822

RESUMO

Biography Binodh DeSilva, PhD Vice President Analytical Strategy & Operations at Bristol-Myer Squibb (BMS) Company in New Brunswick, NJ, USA. She is responsible for leading the regulated analytical (small and large molecule), microbiology and industrial hygiene laboratories as well as the Chemistry, Manufacturing & Controls (CMC) regulatory document management functions in Product Development. She received her Bachelor's in Analytical Chemistry from the University of Colombo, Sri Lanka in 1987, and a PhD in Chemistry (Bioanalytical) in 1994 from the University of Kansas. She was a Post-Doctoral Research Associate in the Pharmaceutical Chemistry Department at the University of Kansas from 1994 to 1995. She was at Procter & Gamble Pharmaceuticals, Norwich, NY, USA, from 1995 to 2001 and at Amgen, Thousand Oaks, CA, USA leading the bioanalytical team from 2001 to 2010. She joined BMS in November of 2010. Dr DeSilva is the Past President of the American Association of Pharmaceutical Scientists. In addition, she is a member of the American Chemical Society, Association for Women in Science, and the Health Care Business Women's Association NY/NJ metro chapter.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Mobilidade Ocupacional , Empatia , Humanos , Indústrias , Mulheres/psicologia
11.
Bioanalysis ; 11(7): 655-656, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997823

RESUMO

Katryn Allen is currently a method development scientist at the PRA Health Sciences Bioanalytical Laboratory in Lenexa, Kansas. Katryn has over 5 years of pharmaceutical industry experience in bioanalytical method development and in the preclinical environment. While at PRA, she has developed ligand-binding assays for pharmacokinetic, pharmacodynamics and immunogenicity end points. She received her PhD in toxicology from the University of Kansas Medical Centre (KS, USA) in 2011.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Indústria Farmacêutica , Humanos , Pesquisadores/psicologia
12.
Bioanalysis ; 11(7): 665-666, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997825

RESUMO

Dr Shane Needham received his BS degree in chemistry from Washington State University and his PhD in chemistry from the University of Rhode Island. Dr Needham is Co-Founder and Chief Scientific Officer of Alturas Analytics, Inc. Dr Needham manages all scientific aspects of the HPLC/MS/MS bioanalytical contract laboratory at Alturas Analytics, Inc. Currently, Dr Needham's work is focused on the development and validation of assays for the determination of therapeutic agents and biomarkers from biological matrices. His laboratory leads in the area of dried blood spot analysis, microflow HPLC-MS/MS and LC-MS/MS of large molecules (Antibody Drug Conjugates, Biomarkers, New Biological Entities etc.) to support Drug Metabolism and Pharmacokinetics research.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Comércio , Humanos , Seleção de Pessoal , Pesquisadores/psicologia , Fatores Socioeconômicos
13.
Bioanalysis ; 11(7): 673-674, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997830

RESUMO

Biography Lauren is Senior Director, Development Biomarkers & Bioanalytical Sciences at Biogen, Cambridge, MA, USA where she leads a team of talented scientists, setting bioanalytical and biomarker strategies and developing pharmacokinetics (PK), immunogenicity and biomarker assays in support of programs at all stages of development, from discovery to post-marketing. Lauren's team currently supports over 40 therapeutic programs, delivering PK and immunogenicity strategy and execution for Biogen's large molecule, antisense oligonucleotide (ASO0 and gene therapy portfolio as well as developing biomarkers in support of multiple sclerosis, immunology, fibrosis, rare and neurodegenerative disease indications. Externally, Lauren engages the broader industry and regulatory agencies as an invited speaker and course instructor at multiple conferences and workshops each year. In concert with her scientific role, Lauren is passionate about people development, has received certification as a Strengths coach and is active in mentorship and career development programs.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Indústria Farmacêutica , Humanos , Motivação , Autoeficácia
14.
Bioanalysis ; 11(7): 671-672, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30997831

RESUMO

Biography Atreyee Sims is an accomplished and committed GCP/GLP trained Project Manager overseeing Phase I-III clinical trials with a strong scientific acumen in pharmacology, microbiology and molecular biology. Project delivery emboldened by 10 years' experience as a Clinical Laboratory Scientist and Microbiologist with more than ten published peer-reviewed manuscripts. Spearheads project leadership role in GCP/GLP and protocol-compliant tracking of project results, implements data collection procedures and verifies project data to ensure timely delivery with the highest priority of both subject safety and data integrity. Proactively identifies, resolves/mitigates and escalates risks and/or issues and responsible for building key partnerships. Excels in team leadership with impeccable organization skills, superior technical writing, communication and problem-solving skills.


Assuntos
Escolha da Profissão , Química Analítica , Identidade de Gênero , Indústria Farmacêutica , Humanos , Desempenho Profissional
15.
Exp Eye Res ; 188: 107656, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31028749

RESUMO

Predictive, preventive and personalized medicine (PPPM) is a current concept in healthcare based on the analysis of biomarkers through non-invasive methods. Biomarkers for inflammation and oxidative stress are especially used for screening. Quantification of tear total protein content is important to identify potential, specific biomarkers, such as malondialdehyde concerning oxidative stress. The Schirmer strip test is an accessible and simple method for tear analysis. However, it is limited by the low concentration of biomarkers in the human tear. In this preliminary study, different procedures were compared for the extraction of tear proteins and malondialdehyde. Schirmer strips were used to obtain tears from healthy subjects. Ionic strength and surfactant agents were assessed, as well as different centrifugation parameters. Finally, several volumes of n-butanol on the process of malondialdehyde extraction were evaluated. The results showed that ionic strength strongly influences the extraction process, although most studies have suggested that surfactant agents are the most relevant factor; the most efficient results were obtained using a 2 M solution of NaCl in phosphate buffered saline. Regarding centrifugation, leaving the Schirmer strip tip left hanging outside the tube cap and using 1000 rpm was the best option, which is a lower centrifugation speed than the usually reported on literature. Moreover, 250 µL was the optimal n-butanol volume for malondialdehyde extraction. The importance of this study relies on the increasing relevance of the biomarkers in the field of PPPM and the need of a standardized method to extract biomarkers from the tears, to optimise its use.


Assuntos
Proteínas do Olho/isolamento & purificação , Malondialdeído/isolamento & purificação , Lágrimas/química , Adulto , Química Analítica , Feminino , Voluntários Saudáveis , Humanos , Masculino , Manejo de Espécimes
16.
Bioanalysis ; 10(20): 1631-1634, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-30354280

RESUMO

Since 2014, the European Bioanalysis Forum organizes a Young Scientist Symposium. The meeting format was created to provide development opportunities for young scientists to engage in international discussions. Creating a peer community of young scientists has been a proven recipe to lower the threshold and promote engagement in this community of young talents. At the same time, the meeting is aimed at stimulating collaboration between the European Bioanalysis Forum and academia.


Assuntos
Bioquímica , Técnicas de Química Analítica , Bioquímica/educação , Bioquímica/métodos , Técnicas de Química Analítica/métodos , Química Analítica/educação , Europa (Continente) , Humanos , Pesquisadores/educação , Manejo de Espécimes/métodos , Detecção do Abuso de Substâncias/métodos
17.
Sensors (Basel) ; 18(9)2018 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-30227614

RESUMO

To non-destructively acquire leaf nitrogen content (LNC), leaf nitrogen accumulation (LNA), leaf area index (LAI), and leaf dry weight (LDW) data at high speed and low cost, a portable apparatus for crop-growth monitoring and diagnosis (CGMD) was developed according to the spectral monitoring mechanisms of crop growth. According to the canopy characteristics of crops and actual requirements of field operation environments, splitting light beams by using an optical filter and proper structural parameters were determined for the sensors. Meanwhile, an integral-type weak optoelectronic signal processing circuit was designed, which changed the gain of the system and guaranteed the high resolution of the apparatus by automatically adjusting the integration period based on the irradiance received from ambient light. In addition, a coupling processor system for a sensor information and growth model based on the microcontroller chip was developed. Field experiments showed that normalised vegetation index (NDVI) measured separately through the CGMD apparatus and the ASD spectrometer showed a good linear correlation. For measurements of canopy reflectance spectra of rice and wheat, their linear determination coefficients (R²) were 0.95 and 0.92, respectively while the root mean square errors (RMSEs) were 0.02 and 0.03, respectively. NDVI value measured by using the CGMD apparatus and growth indices of rice and wheat exhibited a linear relationship. For the monitoring models for LNC, LNA, LAI, and LDW of rice based on linear fitting of NDVI, R² were 0.64, 0.67, 0.63 and 0.70, and RMSEs were 0.31, 2.29, 1.15 and 0.05, respectively. In addition, R² of the models for monitoring LNC, LNA, LAI, and LDW of wheat on the basis of linear fitting of NDVI were 0.82, 0.71, 0.72 and 0.70, and RMSEs were 0.26, 2.30, 1.43, and 0.05, respectively.


Assuntos
Química Analítica , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/metabolismo , Nitrogênio/análise , Oryza/metabolismo , Triticum/metabolismo , Produtos Agrícolas/química , Nitrogênio/metabolismo , Oryza/química , Oryza/crescimento & desenvolvimento , Folhas de Planta/química , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Triticum/química , Triticum/crescimento & desenvolvimento
18.
Anal Bioanal Chem ; 410(24): 6041-6050, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30120497

RESUMO

Nanotechnology is a broad field combining traditional scientific disciplines; however, analytical chemistry plays an important role in material design, synthesis, characterization, and application. This article emphasizes the uniqueness of nanotechnology and the importance of providing high-quality undergraduate research experiences to both attract and retain talented individuals to the field of nanotechnology. In response to this need to develop a strong and sustainable nanotechnology work force, strategies to create authentic research experiences are considered within the framework of an interdisciplinary nanotechnology environment at West Virginia University. The program, named NanoSAFE Research Experiences for Undergraduates (REU), embeds students in different departments at West Virginia University and in research laboratories within the National Institute of Occupational Safety and Health. A large number of participants have little or no prior research experience and a strong effort is made to recruit applicants from under-represented populations. Components designed to foster research proficiency include frequent reporting, a strong peer-network, and training for secondary mentors. Evidence, which includes student publications and assessment findings demonstrating self-efficacy, is discussed to substantiate the viability of the strategies used in the 2016-2018 program. Graphical abstract ᅟ.


Assuntos
Nanotecnologia/educação , Química Analítica/educação , Humanos , Estudos Interdisciplinares , Pesquisa , Estudantes , Universidades , West Virginia
19.
J Vis Exp ; (131)2018 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-29364271

RESUMO

The Yeast Estrogen Screen (YES) is used to detect estrogenic ligands in environmental samples and has been broadly applied in studies of endocrine disruption. Estrogenic ligands include both natural and manmade "Environmental Estrogens" (EEs) found in many consumer goods including Personal Care Products (PCPs), plastics, pesticides, and foods. EEs disrupt hormone signaling in humans and other animals, potentially reducing fertility and increasing disease risk. Despite the importance of EEs and other Endocrine Disrupting Chemicals (EDCs) to public health, endocrine disruption is not typically included in undergraduate curricula. This shortcoming is partly due to a lack of relevant laboratory activities that illustrate the principles involved while also being accessible to undergraduate students. This article presents an optimized YES for quantifying ligands in personal care products that bind estrogen receptors alpha (ERα) and/or beta (ERß). The method incorporates one of the two colorimetric substrates (ortho-nitrophenyl-ß-D-galactopyranoside (ONPG) or chlorophenol red-ß-D-galactopyranoside (CPRG)) that are cleaved by ß-galactosidase, a 6-day refrigerated incubation step to facilitate use in undergraduate laboratory courses, an automated application for LacZ calculations, and R code for the associated 4-parameter logistic regression analysis. The protocol has been designed to allow undergraduate students to develop and conduct experiments in which they screen products of their choosing for estrogen mimics. In the process, they learn about endocrine disruption, cell culture, receptor binding, enzyme activity, genetic engineering, statistics, and experimental design. Simultaneously, they also practice fundamental and broadly applicable laboratory skills, such as: calculating concentrations; making solutions; demonstrating sterile technique; serially diluting standards; constructing and interpolating standard curves; identifying variables and controls; collecting, organizing, and analyzing data; constructing and interpreting graphs; and using common laboratory equipment such as micropipettors and spectrophotometers. Thus, implementing this assay encourages students to engage in inquiry-based learning while exploring emerging issues in environmental science and health.


Assuntos
Química Analítica/educação , Colorimetria/métodos , Cosméticos/química , Disruptores Endócrinos/química , Receptor alfa de Estrogênio/isolamento & purificação , Estrogênios/isolamento & purificação , Preparações Farmacêuticas/química , Cosméticos/análise , Disruptores Endócrinos/análise , Estrogênios/análise , Humanos , Ligantes , Preparações Farmacêuticas/análise
20.
Rocz Panstw Zakl Hig ; 68(3): 309-312, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28895676

RESUMO

Maria Sklodowska was born on November 7, 1867 in Warsaw (Poland). Her parents were teachers. Maria's mother has died in 1878 of tuberculosis. In 1893 and 1894, respectively, Maria was awarded master's degrees in physics and in mathematics from the Sorbonne University. In 1895 Maria married Pierre Curie. In 1897 their daughter Irene was born. Maria investigated rays emitted by uranium salts. She hypothesized that the radiation come from atom and called this phenomenon "radioactivity". In 1898, Maria and Pierre discovered new radioactive elements polonium and radium. In 1902 she isolated pure radium chloride and defined radium atomic mass. In June 1903, Maria supervised by Professor Lippmann was awarded her doctorate in physics from the Sorbonne University of Paris after presentation of the thesis "Investigation of radioactive bodies". In December 1903, Maria was awarded the Nobel Prize in Physics, along with her husband Pierre and Henri Becquerel, for their work on radioactivity. In 1904, the daughter Eve was born. On 19 April 1906, Pierre was killed in a road accident in Paris. In 1910 Maria isolated radium as a pure metal. She also defined an international standard for radioactive emissions (curie), published her fundamental results on radioactivity and textbook of radiology. She also defined the international pattern of radium. In 1911, she won her second Nobel Prize, this time in Chemistry, for her discovery of radium and polonium. In 1914 she was appointed director in the Radium Institute in Paris. During World War I, Maria became the director of the Red Cross Radiology Service and set up France's first military radiology centre. In May 1932 she has attended the official opening ceremony of the Radium Institute in Warsaw. On 4 July 1934, Maria Sklodowska-Curie has died aged 66 years in Sancellemoz sanatorium (France) of aplastic anemia.


Assuntos
Química Analítica/história , Pessoal de Laboratório/história , Radiologia/história , Aniversários e Eventos Especiais , Feminino , França , História do Século XIX , História do Século XX , Humanos , Prêmio Nobel , Polônia , Mulheres Trabalhadoras/história
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA