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1.
Ann Lab Med ; 42(1): 24-35, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34374346

RESUMO

Background: Laboratory parameter abnormalities are commonly observed in COVID-19 patients; however, their clinical significance remains controversial. We assessed the prevalence, characteristics, and clinical impact of laboratory parameters in COVID-19 patients hospitalized in Daegu, Korea. Methods: We investigated the clinical and laboratory parameters of 1,952 COVID-19 patients on admission in nine hospitals in Daegu, Korea. The average patient age was 58.1 years, and 700 (35.9%) patients were men. The patients were classified into mild (N=1,612), moderate (N=294), and severe (N=46) disease groups based on clinical severity scores. We used chi-square test, multiple comparison analysis, and multinomial logistic regression to evaluate the correlation between laboratory parameters and disease severity. Results: Laboratory parameters on admission in the three disease groups were significantly different in terms of hematologic (Hb, Hct, white blood cell count, lymphocyte%, and platelet count), coagulation (prothrombin time and activated partial thromboplastin time), biochemical (albumin, aspartate aminotransferase, alanine aminotransferase, lactate, blood urea nitrogen, creatinine, and electrolytes), inflammatory (C-reactive protein and procalcitonin), cardiac (creatinine kinase MB isoenzyme and troponin I), and molecular virologic (Ct value of SARS-CoV-2 RdRP gene) parameters. Relative lymphopenia, prothrombin time prolongation, and hypoalbuminemia were significant indicators of COVID-19 severity. Patients with both hypoalbuminemia and lymphopenia had a higher risk of severe COVID-19. Conclusions: Laboratory parameter abnormalities on admission are common, are significantly associated with clinical severity, and can serve as independent predictors of COVID-19 severity. Monitoring the laboratory parameters, including albumin and lymphocyte count, is crucial for timely treatment of COVID-19.


Assuntos
COVID-19 , Análise de Dados , Humanos , Laboratórios , Masculino , Pessoa de Meia-Idade , República da Coreia/epidemiologia , Estudos Retrospectivos , SARS-CoV-2
2.
Ann Lab Med ; 42(1): 89-95, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34374353

RESUMO

Background: Total laboratory automation (TLA) is an innovation in laboratory technology; however, the high up-front costs restrict its widespread adoption. To examine whether the capital investment for TLA is worthwhile, we analyzed its clinical- and cost-effectiveness for the expected payback period. Methods: Clinical chemistry tests and immunoassays performed in the clinical laboratory of a tertiary care hospital were divided into a post-TLA group, including 1,182,419 tests performed during December 2019, and a pre-TLA group, including 1,151,501 tests performed during December 2018. Laboratory information system data were used to measure clinical effectiveness, and depreciation data were used to calculate TLA costs. Results: Laboratory performance improved after TLA adoption in all four key performance indicators: mean turn-around time (TAT), representing the timeliness of result reporting, decreased by 6.1%; the 99th percentile of TAT, representing the outlier rate, decreased by 13.3%; the TAT CV, representing predictability, decreased by 70.0%; and weighted tube touch moment (wTTM), representing staff safety, improved by 77.6%. Based on these effectiveness results, economic evaluation was performed using two approaches. First, the incremental cost-effectiveness ratio and wTTM were used as the most cost-effective performance indicators. Second, the expected payback period was calculated. Considering only staff cost reduction, it was anticipated that 4.75 yrs would be needed to payback the initial investment. Conclusions: TLA can significantly enhance laboratory performance, has a relatively quick payback period, and can reduce total hospital expenses in the long term. Therefore, the capital investment for TLA adoption is considered to be worthwhile.


Assuntos
Automação Laboratorial , Serviços de Laboratório Clínico , Análise Custo-Benefício , Humanos , Laboratórios , Centros de Atenção Terciária
3.
Food Chem ; 367: 130748, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34375894

RESUMO

The development of a collaborative study as a requirement for the preparation of a laboratory reference material candidate is reported in this paper. The evaluation was performed by 13 laboratories invited to quantify the calcium, potassium, magnesium, phosphorus, copper, iron, manganese and zinc; 8 of them presented results for all the analytes under investigation. The data were statistically analyzed by applying the z-score robust technique as recommended by ISO Guide 35. For the potassium element, laboratories 4 and 13 presented questionable results. Laboratory 5 proved to be unsatisfactory for calcium and zinc. ANOVA-PCA and DD-SIMCA were also applied to evaluate stability and interlaboratory studies results, respectively. It has been demonstrated that multivariate data analysis can be successfully applied as an alternative method to the recommendations made by ISO 13528 and ISO Guide 35 with defined confidence intervals.


Assuntos
Laboratórios , Zea mays , Análise de Variância , Cálcio , Cobre , Farinha , Magnésio
4.
Ann Lab Med ; 42(1): 1-2, 2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34374344

Assuntos
Laboratórios , Humanos
5.
Talanta ; 237: 122916, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34736654

RESUMO

Herein, we show differences in blood serum of asymptomatic and symptomatic pregnant women infected with COVID-19 and correlate them with laboratory indexes, ATR FTIR and multivariate machine learning methods. We collected the sera of COVID-19 diagnosed pregnant women, in the second trimester (n = 12), third-trimester (n = 7), and second-trimester with severe symptoms (n = 7) compared to the healthy pregnant (n = 11) women, which makes a total of 37 participants. To assign the accuracy of FTIR spectra regions where peak shifts occurred, the Random Forest algorithm, traditional C5.0 single decision tree algorithm and deep neural network approach were used. We verified the correspondence between the FTIR results and the laboratory indexes such as: the count of peripheral blood cells, biochemical parameters, and coagulation indicators of pregnant women. CH2 scissoring, amide II, amide I vibrations could be used to differentiate the groups. The accuracy calculated by machine learning methods was higher than 90%. We also developed a method based on the dynamics of the absorbance spectra allowing to determine the differences between the spectra of healthy and COVID-19 patients. Laboratory indexes of biochemical parameters associated with COVID-19 validate changes in the total amount of proteins, albumin and lipase.


Assuntos
COVID-19 , Feminino , Humanos , Laboratórios , Aprendizado de Máquina , Gravidez , Gestantes , SARS-CoV-2 , Soro , Análise Espectral , Vibração
6.
Ann Lab Med ; 42(2): 121-140, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34635606

RESUMO

The process of method development for a diagnostic assay based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) involves several disparate technologies and specialties. Additionally, method development details are typically not disclosed in journal publications. Method developers may need to search widely for pertinent information on their assay(s). This review summarizes the current practices and procedures in method development. Additionally, it probes aspects of method development that are generally not discussed, such as how exactly to calibrate an assay or where to place quality controls, using examples from the literature. This review intends to provide a comprehensive resource and induce critical thinking around the experiments for and execution of developing a clinically meaningful LC-MS/MS assay.


Assuntos
Serviços de Laboratório Clínico , Espectrometria de Massas em Tandem , Cromatografia Líquida , Humanos , Laboratórios , Controle de Qualidade
7.
Ann Lab Med ; 42(2): 150-159, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34635608

RESUMO

Background: Results from laboratories using multiple instruments should be standardized or harmonized and comparability-verified for consistent quality control. We developed a simple frequent comparability verification methodology applicable to large healthcare centers using multiple clinical chemistry instruments from different manufacturers. Methods: Comparability of five clinical chemistry instruments (Beckman Coulter AU5800, Abbott Architect Ci16000, two Siemens Vista 1500, and Ortho Vitros 5600) was evaluated from 2015 to 2019 for 12 clinical chemistry measurements. Pooled residual patient samples were used for weekly verifications. Results from any instrument exceeding the allowable verification range versus the results from the comparative instrument (AU5800) were reported to clinicians after being multiplied by conversion factors that were determined via a linear regression equation obtained from simplified comparison. Results: Over the five-year study period, 432 weekly inter-instrument comparability verification results were obtained. Approximately 58% of results were converted due to non-comparable verification. Expected average absolute percent bias and percentage of non-comparable results for non-converted and converted results after conversion action were much lower than those for data measured before conversion action. The inter-instrument CV for both non-converted and converted results after conversion action was much lower than that for measured data before conversion action for all analytes. Conclusions: We maintained within-laboratory comparability of clinical chemistry tests from multiple instruments for five years using frequent low-labor periodic comparability verification methods from pooled residual sera. This methodology is applicable to large testing facilities using multiple instruments.


Assuntos
Química Clínica , Laboratórios , Testes de Química Clínica , Atenção à Saúde , Humanos , Controle de Qualidade
8.
Ann Lab Med ; 42(2): 169-177, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34635610

RESUMO

Background: Hemolysis is the most common type of preanalytical interference. Cut-offs based on the hemolysis index level can be established using different approaches. The Working Group for Preanalytical Phase of the European Federation of Laboratory Medicine has developed a protocol for hemolysis management based on cut-offs estimated from biological variation (BV) and the use of interpretative comments. We developed and assessed the implementation of the protocol in our laboratory. Methods: Hemolysates from whole blood were prepared following the Meites method, and pooled serum samples with known Hb concentrations were prepared. For each analyte (42 ), interferograms were generated and used to establish cut-offs: desirable analytical quality specification and reference change value. This protocol was assessed, both pre- and post-implementation, according to expert rules in the Laboratory Information System. Results: Among the analytes evaluated, we selected those that showed the highest degree of hemolysis interference: lactate dehydrogenase (LDH), aspartate aminotransferase, direct bilirubin, potassium, and folic acid. The cut-offs for LDH and direct bilirubin were the lowest. Only 28.16% of all LDH values were adequately reported in the pre-implantation retrospective study, but this percentage improved in the post-implementation stage. Conclusions: The development and implementation of a harmonized protocol for hemolysis management based on BV cut-offs and result reporting significantly improve hemolysis detection and lead to a decrease in the number of hemolyzed samples over time.


Assuntos
Serviços de Laboratório Clínico , Hemólise , Testes Hematológicos , Humanos , Laboratórios , Estudos Retrospectivos
9.
J Hazard Mater ; 421: 126717, 2022 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-34339992

RESUMO

Laboratory scale recycling of marine plastic litter consisting of polyethylene terephthalate (PET) bottle sorting, pyrolysis and chemical vapor deposition (CVD) was conducted to identify the technical and environmental implications of the technology when dealing with real waste streams. Collected seashore and underwater plastics (SP and UP, respectively) contained large quantities of PET bottles (33.2 wt% and 61.4 wt%, respectively), suggesting PET separation was necessary prior to pyrolysis. After PET sorting, marine litter was converted into pyrolysis oil and multi-walled carbon nanotubes (MWCNTs). Water-based washing of litter prior to pyrolysis did not significantly change the composition of pyrolysis products and could be avoided, eliminating freshwater consumption. However, distinct differences in oil and MWCNT properties were ascribed to the variations in feedstock composition. Maintaining consistent product quality would be one of challenges for thermochemical treatment of marine litter. As for the environmental implications, life cycle assessment (LCA) demonstrated positive benefits, including improved climate change and fossil depletion potentials. The highest positive environmental impacts were associated with MWCNT production followed by pyrolysis oil and PET recovery. The benefits of proposed approach combining PET sorting, pyrolysis and CVD allowed to close the waste loop by converting most of the marine litter into valuable products.


Assuntos
Nanotubos de Carbono , Plásticos , Laboratórios , Polietilenotereftalatos , Reciclagem
10.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120235, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34371314

RESUMO

Curcumin is a natural product that is frequently utilized in cancer prevention and treatment. The significant benefit of vegetable-derived nutraceuticals in combination with widespread cytostatic medication such as ponatinib is to reduce toxicity and side effects. In this paper, we focus the study on analytical quantification of ponatinib and curcumin through highly sensitive synchronous spectrofluorometric method. Applying this method at Δλ = 160 nm, each of ponatinib and curcumin could be measured at 303 and 412 nm without interference from each others. The diverse experimental factors impacting the performance of the method were studied and optimized. The method exhibited a reasonable linearity in the ranges of 5.0-60.0 and 10.0-200.0 ng/mL for ponatinib and curcumin, respectively with detection limits of 1.48 and 1.22 ng/mL and quantitation limits of 4.49 and 3.68 ng/mL, respectively. The anticipated method was employed for the assessment and evaluation of the studied drugs in the spiked human plasma samples. The mean % recoveries in plasma samples (n = 6) for each of ponatinib and curcumin were 99.84 ± 1.86 and 100.06 ± 2.72, accordingly. The developed method was validated in conformity with the requirements of International Council of Harmonization (ICH).


Assuntos
Curcumina , Humanos , Imidazóis , Laboratórios , Piridazinas , Espectrometria de Fluorescência
11.
Chemosphere ; 287(Pt 1): 131883, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34818820

RESUMO

Ecological risk assessments of chemicals are frequently based on laboratory toxicity data from a small number of model species that may be reared in labs for years or decades. These populations can undergo many processes in the lab including artificial selection, founder effect, and genetic drift, and may not adequately represent their wild counterparts, potentially undermining the goal of protecting natural populations. Here we measure variation in lethality to copper chloride among strains of an emerging model species in toxicology, Caenorhabditis elegans. We tested four wild strains from Chile, Germany, Kenya, and Madeira (Portugal) against several versions of the standard laboratory N2 strain from Bristol, UK used in molecular biology. The four wild strains were more sensitive than any of the N2 strains tested with copper. We also found that the standard N2 strain cultured in the laboratory for >1 year was less sensitive than a recently cultured N2 strain as well as a cataloged ancestral version of the N2 strain. These results suggest that toxicologists should be cognizant of performing toxicity testing with long-held animal cultures, and should perhaps use multiple strains as well as renew cultures periodically in the laboratory. This study also shows that multi-strain toxicity testing with nematodes is highly achievable and useful for understanding variation in intra- and interspecific chemical sensitivity.


Assuntos
Caenorhabditis elegans , Nematoides , Animais , Caenorhabditis elegans/genética , Cobre/toxicidade , Laboratórios , Testes de Toxicidade
12.
Chemosphere ; 287(Pt 2): 132191, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34509021

RESUMO

As the presence of emergent contaminants in wastewater, such as antibiotics, has become a threat for public health, the evaluation of strategies to treat them has been gaining importance. A critical example of this situation can be found in wastewaters coming from the pharmaceutical industry, where high concentrations of antibiotics are sometimes accompanied by high organic contents. Even the agroindustry can be affected by a similar problem when cattle infections are treated with antibiotics and part of the antibiotic-contaminated milk has to be wasted. With these situations in mind, in the present study we evaluated a progressive acclimation strategy for a granular sludge in a UASB reactor treating a high organic-content synthetic wastewater contaminated with azithromycin. In parallel, we tested a previously reported low-cost method for azithromycin determination by spectrophotometry, obtaining results comparable with liquid chromatography coupled to mass spectrometry. Although azithromycin has been reported as recalcitrant and resistant to biological degradation, the antibiotic was removed with efficiencies over 50% for wastewater with 10 mg L-1 of azithromycin and a COD of more than 4000 mgO2 L-1. Furthermore, efficiencies over 40% were achieved for wastewater with higher azithromycin concentrations (80 mg L-1) and a COD of 20,000 mgO2 L-1. A careful acclimation strategy permitted the partial removal of azithromycin from wastewater when treating concentrations comparable and higher than what would be expected for domestic and hospital wastewaters, even when its chemical oxygen demand is considerably higher than the average maximum of around 1000 mgO2 L-1.


Assuntos
Azitromicina , Águas Residuárias , Anaerobiose , Animais , Reatores Biológicos , Bovinos , Laboratórios , Esgotos , Eliminação de Resíduos Líquidos
13.
Braz J Biol ; 83: e03316, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34730713

RESUMO

Behavioral lab bioassays involving termites must be promptly performed to allow intended observations prior to death from dissecation, typical of these soft-bodied insects. To this end, topic markers have been proposed as an alternative to histological stains which, while not always toxic are inevitably lengthy to apply. Among recommended topic markers, gouache is easy to apply, dries out quickly, but it is known affect termites in the long run, being suitable only to short-term bioassays. Its alternative, colored glue, is also easy to apply, but it takes long to dry and it is too dense and heavy, being thus prone to affect termite walking patterns. Here we tested a mix of gouache and colored glue aiming to combine the qualities of both into a suitable topical marker for Cornitermes cumulans termites. Similar patterns of survival presented by marked and unmarked termites ruled out concerns about toxicity of this mixture. Such results were consistent across distinct group densities evidencing that the mixture does not interfere with, nor it is affected by, crowding effects. Because crowding regulates interindividual interactions and these underlie most behaviors, the mixture can be thought to be suitable to behavioral studies. We argue that this 1:2 glue:gouache mixture is an excellent alternative to mark termites for lab bioassays. Being atoxic, cheap, easy to apply, and non-invasive, this mixture may happen to be useful not only for termites but also in bioassaying other similarly soft-bodied insects.


Assuntos
Baratas , Isópteros , Animais , Bioensaio , Laboratórios
14.
Saudi Med J ; 42(11): 1165-1172, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34732547

RESUMO

OBJECTIVES: To validate C-reactive protein (CRP), red cell distribution width (RDW), and neutrophil lymphocyte ratio (NLR) for both serious outcomes and length of hospital stay (LOS) among hospitalized coronavirus disease-19 (COVID-19) patients. METHODS: Laboratory data of adult COVID-19 patients (n=74) was collected in this retrospective cohort. Logistic regression was employed for risk factor evaluation and receiver operating curve was used for comparison of these risk factors for the prediction of serious outcome. Multiple regression was applied to determine the association between routine analytes and LOS. RESULTS: Higher levels of CRP (3 times), white blood cells (20%), and neutrophil counts (40%) were seen in the serious category. Odds ratio for CRP for the serious outcome was 1.052 (p=0.007) and RDW for the serious outcome was 1.218 (p=0.040) in unadjusted model and odds ratio for CRP for the serious outcome was 1.048 (p=0.024) and for RDW 1.286 (p=0.023) in adjusted model. In a multivariate regression analysis for the LOS of the unadjusted models consisting of NLR, monocyte lymphocyte ratio (MLR) and platelet lymphocyte ratio (PLR), the beta coefficients (BC) for the CRP were 0.006 (NLR), 0.005 (MLR) and 0.006 (PLR), whereas -0.029 (NLR), -0.034 (MLR) and -0.027 (PLR) were BCs for mean corpuscular hemoglobin concentration (MCHC). Additionally, in adjusted models, the BCs for MCHC were -0.044 (NLR), -0.047 (MLR) and -0.043 (PLR). However, the CRP was consistent with 0.004 (BC) in all models. CONCLUSION: We observed that CRP is a better predictor than RDW and NLR for serious outcome among COVID-19 patients. Besides, CRP was positively, whereas MCHC was negatively associated with LOS.


Assuntos
COVID-19 , Laboratórios , Plaquetas , Humanos , Tempo de Internação , Linfócitos , Neutrófilos , Prognóstico , Estudos Retrospectivos , SARS-CoV-2
15.
Acta Biomed ; 92(5): e2021297, 2021 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-34738591

RESUMO

BACKGROUND: The coronary no-reflow phenomenon is an adverse complication of percutaneous coronary interventions (PCI) which significantly worsens the outcome and survival. In this study, we have evaluated the correlation of no-reflow phenomenon with demographic, biochemical and anatomical factors. METHODS: We included 306 patients (193 male) with acute ST-elevation myocardial infarction (STEMI) who undergone primary PCI in our center. Demographic factors, as well as biochemistry test results were obtained. Also, the Thrombolysis in Myocardial Infarction (TIMI) grade and TIMI frame count (TFC) was measured. The correlation of no-reflow phenomenon with demographic, biochemical and anatomical factors was analyzed. RESULTS: Patients with a mean age of 56.41 ± 11.8 years were divided into two groups depending on the TIMI score (Group 1 or Normal flow and Group 2 or No-reflow). Symptom-to-procedure time, door-to-procedure time, serum creatinine level, hs-CRP level, and Neutrophil to Lymphocyte Ratio (NLR) were significantly higher among group 2. TFC had negative significant correlation with male gender, and positive significant correlation with age, diabetes mellitus, hs-CRP level, WBC count, and NLR. Age of more than 62.5 years and serum creatinine level of more than 0.89 mg/dL can optimally predict the no reflow phenomena. CONCLUSIONS: According to our results, it seems that female gender, older ages, DM, multi-vessel involvement, delayed reperfusion, and increased NLR can predict the risk of no-reflow after primary PCI in the setting of Acute Myocardial Infarction.


Assuntos
Fenômeno de não Refluxo , Intervenção Coronária Percutânea , Infarto do Miocárdio com Supradesnível do Segmento ST , Adulto , Idoso , Angiografia Coronária , Demografia , Feminino , Humanos , Laboratórios , Masculino , Pessoa de Meia-Idade , Fenômeno de não Refluxo/epidemiologia , Fenômeno de não Refluxo/etiologia
16.
Stomatologiia (Mosk) ; 100(5): 58-61, 2021.
Artigo em Russo | MEDLINE | ID: mdl-34752036

RESUMO

OBJECTIVE: To study the physical and mechanical properties of the new basic light polymer material in clinical conditions in pediatric orthodontic patients. MATERIAL AND METHODS: The strength properties of 113 basic orthodontic devices made of photopolymer material without reinforcement with a screw, vestibular arch, and clasps t (55 devices, 30 of them for the upper jaw, 25 for the lower jaw) and with quartz mesh reinforcement of the base (58 devices: 30 for the upper jaw, 28 for the lower jaw) were studied in 60 children aged 3 to 12 years in the periods of temporary and removable bite. RESULTS: In total, breakdowns of the base in devices without reinforcement were noted in 5.5% of cases. Our experimental studies have shown a significant improvement in the strength properties (modulus of elasticity, crack resistance, bending strength, maximum bending strain) when reinforcing the base polymer quartz mesh more than times. The clinical part of the study effectively reflects the positive trends in the reinforcement of the base from. A specific symptom complex of allergic reactions when wearing removable devices made of light-curing polymer was not detected. The observations continued for two years. CONCLUSION: The obtained results of the strength properties of the reinforced base from the light-cured polymer and the absence of breakdowns, cracks and other deformations make it possible to produce reliable extensible orthodontic removable structures and devices, as well as to recommend this bioinert material for the correction of dental anomalies in children in the clinic of an orthodontist. This will improve the quality of services provided, significantly reduce the time of orthodontic treatment of dental anomalies in children.


Assuntos
Luzes de Cura Dentária , Cura Luminosa de Adesivos Dentários , Criança , Oclusão Dentária , Humanos , Laboratórios , Polímeros
17.
Stomatologiia (Mosk) ; 100(5): 88-95, 2021.
Artigo em Russo | MEDLINE | ID: mdl-34752042

RESUMO

THE AIM OF THE STUDY: Was to review laboratory methods measuring the bond strength of adhesive dental materials to hard tooth tissues. The paper points out the impact of test conditions on the measured strength of adhesive bond to dentine and enamel. Various in vitro studies are discussed, including the analyses of fracture surfaces and test-induced stresses. These studies are found to be helpful to identify particularities, advantages, and limitations of each approach. We present a systematic picture of available bond-strength data aimed at revealing general trends in adhesive performance. No clear superiority of any particular method for determining the bond strength between restoration materials and tooth tissues can be identified on the basis of reviewed literature. Therefore, no single universal approach can be at present proposed for determining the strength of adhesive bond.


Assuntos
Colagem Dentária , Cimentos de Resina , Resinas Compostas , Cimentos Dentários , Materiais Dentários , Análise do Estresse Dentário , Dentina , Humanos , Laboratórios , Teste de Materiais , Resistência ao Cisalhamento
18.
Acta Derm Venereol ; 101(11): adv00587, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34724070

RESUMO

Sensitive skin is a prevalent syndrome, characterized by discomfort in response to mild stimuli, which impacts on quality of life. Pruritus is one of the major symptoms of sensitive skin. However, the pathomechanism of sensitive skin is insufficiently understood. As an experimental model for pruritus, the cowhage skin prick test might provide insight into the understanding of sensitive skin. This study aimed to specify the characteristics of cowhage-induced pruritus in sensitive skin. Female volunteers, 20 with sensitive skin and 20 controls, were recruited. Self-report questionnaires were distributed and the responses evaluated; moreover, alongside assessments by dermatologists, skin physiology assessments, lactic acid sting test, capsaicin test and cowhage skin challenge were performed. Pruritus in sensitive skin was perceived as more intense and longer-lasting than in normal skin, with different qualities of accompanying sensations. Cowhage skin challenge results showed moderate consistency with clinical assessments. The results suggest that cowhage skin challenge could be a new tool for the assessment of sensitive skin.


Assuntos
Laboratórios , Mucuna , Histamina , Humanos , Prurido/diagnóstico , Qualidade de Vida , Pele
19.
Clin Lab ; 67(11)2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34758221

RESUMO

BACKGROUND: Automated microscopic platforms are increasingly used in clinical laboratories for rapid analysis of samples. However, it is important to present the results quantitatively or semiquantitatively because automated platforms use various technologies for analysis as well as different sediment preparation methods. The results of cell counting using an on screen image review program for the cobas u 701 analyzer (Roche Diagnostics Interna-tional, Rotkreuz, Switzerland) differed from those obtained by manual microscopic examination (MME). This study was performed to investigate the difference of results among analyzer, on-screen image review and MME. METHODS: Freshly collected urine specimens from outpatients were used. We calculated the mean, standard deviation, and 95% confidence interval for red and white blood cell (RBC/WBC) quantitative results obtained using the cobas u 701 analyzer. These results were compared to those obtained by manual counting. RBC and WBC counts determined with the cobas u 701 analyzer were compared to those obtained by MME per unit field. RESULTS: The semiquantitative results of MME were graded as 0 - 2, 3 - 5, 6 - 10, 11 - 20, 21 - 30, and many or numerous cells/high power field (HPF). The RBC and WBC counts determined by image analyses showed the tendency to be one grade higher than those from MME in the range of 3 to 5/HPF to many/HPF. The results of nearly all samples with 0 - 2/HPF and numerous/HPF for RBC and WBC counts were consistent with the grade found by MME. CONCLUSIONS: The one-grade difference may have been caused by the differences of preanalytical factors in the sample volume, centrifugal force, urine concentration ratio, or sediment volume/area of the slide. When reporting the results of image analyses, RBC and WBC counts should be raised by one grade to compensate for MME. Each laboratory needs to verify the on-screen review of images corresponding to the microscopic field of view according to the clinical laboratory's specific preanalytical practices.


Assuntos
Microscopia , Urinálise , Laboratórios , Contagem de Leucócitos , Leucócitos , Urina
20.
Clin Lab ; 67(11)2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34758235

RESUMO

BACKGROUND: Patients with Kawasaki disease (KD) may develop cardiovascular complications in the presence of predictive factors, including young age < 6 months, male gender, unfavorable response to intravenous immunoglobulin (IVIG), low albuminemia, thrombocytosis, fever over 8 days, increased C-reactive protein (CRP), elevated levels of 25 OH vitamin D3, elevated levels of fibroblast growth factor 23 (FGF23), elevated D-dimers, elevated ferritin. The objectives of this study were to determine the laboratory negative predictive factors for the occurrence of cardiac complications in children with KD. Studies in the literature that dealt with these predictive factors were analyzed. METHODS: We followed the studies published in PubMed over a 10-year period. Seventy articles were reviewed and, after applying the inclusion and exclusion criteria, 20 articles were selected. RESULTS: We evaluated the population studies which showed factors can predict the occurrence of heart complications. These factors were different depending on age and depending on resistance to IVIG treatment. CONCLUSIONS: Some biological parameters such as low albumin, thrombocytosis, increased CRP, elevated levels of 25 OH vitamin D3, elevated levels of FGF23, elevated D-dimers, and elevated ferritin could be considered as laboratory negative predictive factors for CAL.


Assuntos
Síndrome de Linfonodos Mucocutâneos , Calcifediol , Criança , Febre , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Lactente , Laboratórios , Masculino , Síndrome de Linfonodos Mucocutâneos/complicações , Síndrome de Linfonodos Mucocutâneos/diagnóstico , Síndrome de Linfonodos Mucocutâneos/tratamento farmacológico
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