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West Indian med. j ; 47(suppl. 1): 30-1, Mar. 5-8, 1998.
Artigo em Inglês | MedCarib | ID: med-1550


Nitric oxide is a pathogenic factor of inflammatory islet cell death in type 1 diabetes. An early event in the pathogenesis of insulin-dependent diabetes mellitus (IDDM) is intraislet accumulation of activated macrophages. Their secretory products such as nitric oxide (NO) are found to play a crucial role in islet destruction. Macrophage activity and progressive islet cell destruction persist during a long period of chronic inflammatory events preceding diabetes, suggesting the presence of nitric oxide contributing to continued immunostimulation. It has been shown previously that the nitric oxide donor, S-nitrosoglutathione (GSNO) caused persistent postprandial hyperglycaemia in normal healthy dogs at 35 and 50mg/kg. parallel with an increase in plasma nitrate concentration and decrease in insulin secretion. This study was designed to investigate differences in cellular binding of insulin in dogs administered with the GSNO. A time course assay of insulin binding, to isolate erythrocytes and mononuclear leucocytes from dog administered with GSNO, was done during the oral glucose tolerance test (OFTT). The erthrocyte receptor assay performed was the methodology used by Ghambir et al (1977). A modification was also done for mononuclear leucocytes insulin binding assay. The plasma glucose levels were measured by the glucose oxidase method, while the insulin levels were determined by radio-immunoassay. The results of these studies show that erythrocytes and mononuclear leucocytes from dogs administered with GSNO have decreased ability to bind insulin when compared to erythrocytes and mononuclear leucocytes from the controls. In dogs administered with GSNO, there was less binding of erythrocytes and mononuclear leucocytes, 44 percent and 29 percent respectively, when compared to the bound free ratio value of the controls. The data also shows that erythrocytes and mononuclear leucocytes from dogs administered with GSNO have 256 and 10.7x10 insulin receptor sites per cell, respectively, compared with 296 and 18.4x10 per cell for the control (P<0.05). Competitive inhibition studies using unlabelled insulin indicate that the affinity of insulin for its receptor on erythrocytes from dogs administered with GSNO was also significantly different from the controls, while that of mononuclear leucocytes from both group was comparable.(AU)

Cães , 21003 , Eritrócitos/efeitos dos fármacos , Insulina/uso terapêutico , Leucócitos Mononucleares/efeitos dos fármacos , Glutationa/efeitos dos fármacos , Compostos Nitrosos/uso terapêutico , Óxido Nítrico/uso terapêutico
West Indian med. j ; 41(1): 23-6, Mar. 1992.
Artigo em Inglês | MedCarib | ID: med-11741


Subacute intraperitoneal administration of the lipid portion of the unripe ackee arillus, referred to as "ackee oil", resulted in marked neutropenia (p<0.001) and increase in platelets (p<0.01) without anaemia, in rats. Blood urea, sodium amd aspartate aminotransferase levels were significantly decreased but glucose and bilirubin levels were similar to those of controls. The lungs showed areas of petechial haemorrhaghes and a dose-related perivascular and peribronchial mononuclear cell infiltration. The pulmonary toxicity may be interpreted as a hypersensitive reaction to ackee oil. Further research is in progress on the neutropenic effects of ackee oil. (AU)

Ratos , 21003 , Óleos Vegetais/toxicidade , Neutropenia/induzido quimicamente , Jamaica , Leucócitos Mononucleares/efeitos dos fármacos , Extratos Vegetais/toxicidade , Óleos Vegetais/metabolismo , Contagem de Plaquetas/efeitos dos fármacos , Ratos Endogâmicos
West Indian med. j ; 39(3): 144-7, Sept., 1990.
Artigo em Inglês | MedCarib | ID: med-12486


This study was designed to investigate any differences in cellular binding of insulin between phasic insulin-dependent (malnutrition-related) diabetes mellitus (PIDDM) and insulin-dependent, non-insulin-dependent, and normal controls. Isolated, washed red and white blood cells obtained after 12-14 hr fast, were separately incubated with varying concentrations of non-radioactive insulin, and a fixed quantity of radioactively labelled insulin. After the 3-hr incubation, cells were washed with buffer, and radioactivity determined on an autogamma counter. Percentage binding, receptor sites number an affinity were all determined by linear regression of the Scatchard plot. Fasting plasma insulin and glucose levels were also assayed. The results obtained showed decreased binding of insulin in red blood cells (11.3 ñ 1.3 percent) and white blood cells 2.9 ñ 0.5 percent) in PIDDM. This was due to decreased receptor sites (red blood cells 39 ñ 11; white blood cells 0.5 ñ 0.11 x 10 to the 4th) as well as decreased affinity (red blood cells 0.14 ñ 0.03 x 10 to the 9 M to the -1) when compared to the normal and diabetes (malnutrition-related diabetes mellitus) is characterized by decreased red and white cellular binding to insulin, in addition to decreased production of insulin.(AU)

Humanos , Diabetes Mellitus/sangue , Eritrócitos/metabolismo , Leucócitos Mononucleares/metabolismo , Transtornos Nutricionais/metabolismo , Receptor de Insulina/análise , Diabetes Mellitus/etiologia , /metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Transtornos Nutricionais/complicações
Blood ; 75(2): 428-33, Jan. 15, 1990.
Artigo em Inglês | MedCarib | ID: med-10028


Human T-cell lymphotropic virus type I (HTLV-I) proviral integration status was examined by Southern blot analysis in peripheral blood mononuclear cell (PBMC) DNA from patients presenting a tropical spastic paraparesis (TSP) and serological evidence of HTLV-I infection. Surface phenotype and morphological aspects of PBMC were also studied. A polyclonal HTLV-I proviral integration was found in the PBMC of the 10 patients studied irrespective of their geographical origin (French West Indies, French Guiana, and Africa), the duration of their clincal illness, or the HTLV-I antibody titer. Furthermore, by dilution experiments and hypothesizing that only one copy of HTLV-I proviral DNA is present in one call, we estimated that this HTLV-I integration is present in 3 percent to 15 percent of their PBMC. All 10 TSP/HTLV-I patients studied had an average of 10 percent of thier lymphocytes abnormal, presening either a misshapen nucleus or an adult T-cell leukemia/lymphoma(ATL)-like feature. Moreover, an elevated CD4/CD8 ratio associated with the presence of activated T cells with a high level of DR expression was observed in most patients. The significant frequency of viral-positive PBMC and the important load of HTLV-I proviral DNA that we observed in TSP/HTLV-I patients might play an important role in the pathogenesis of this recently identified clinico-virological entity. (AU)

Humanos , Vírus Linfotrópico T Tipo 1 Humano/genética , Leucócitos Mononucleares/microbiologia , Paraparesia Espástica Tropical/microbiologia , Anticorpos Monoclonais , Antígenos CD , Southern Blotting , Células Clonais , Sondas de DNA , DNA Viral/análise , Guiana Francesa , Anticorpos Antideltaretrovirus/análise , Costa do Marfim , Martinica , Mapeamento por Restrição , Proteínas do Envelope Viral/genética , Índias Ocidentais , República Democrática do Congo
Proc Natl Acad Sci U S A ; 86(6): 2021-5, Mar. 1989.
Artigo em Inglês | MedCarib | ID: med-12266


The isolation and characterization of a human T-cell lymphotrophic virus type I (HTLV-I) from cerebrospinal fluid of a Jamaican patient with tropical spastic paraparesis is described. The virus isolate is a typical type C retrovirus as seen by electron microscopy and is related to prototype HTLV-I isolated from patients with adult T-cell leukemia but is not identical to this prototype HTLV-I as seen by restriction enzyme mapping.(AU)

Humanos , Idoso , Feminino , Líquido Cefalorraquidiano/microbiologia , Vírus Linfotrópico T Tipo 1 Humano/isolamento & purificação , Paraparesia Espástica Tropical/microbiologia , Células Cultivadas , Enzimas de Restrição do DNA , DNA Viral/análise , Imunofluorescência , Jamaica , Leucemia , Leucemia de Células T/microbiologia , Leucócitos Mononucleares/microbiologia , Microscopia Eletrônica , Paraparesia Espástica Tropical/imunologia , DNA Polimerase Dirigida por RNA/análise