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2.
Kingston; s.n; Dec. 1972. 185 p. ills, tab.
Tese em Inglês | MedCarib | ID: med-13707

RESUMO

With increasing interest in mutagenicity an array of agents such as chemicals and drugs are being defined as chromosome damaging agents. The activities of these agents were reviewed. An intensive investigation of the mutagenic potential of fulvine was undertaken for three reasons. 1. Fulvine is still widely consumed in Jamaica as a herbal remedy which causes a well defined pathological condition - veno - occlusive disease of the liver (VOD). 2. Fulvine was found to cause chromosome damage in previous screening experiments. 3. Fulvine is a member of the pyrrolizidine group of alkaloids which are known to have mutagenic effects. The mutagenic activities were investigated by in vivo studies in children who have recovered from VOD and experimentally in rats. Serial examination of 3 members of a family recently recovered showed a decline of the percentage of cells with damage to normal over a five month period. No abnormalities were detected in 7 recovered children. Fulvine was administered to rats at varying dosages of 0.08 mg/gm, 0.04 mg/gm, 0.02 mg/gm, 0.01 mg/gm, and repeated administrations of 0.04 mg/gm body weight. Chromosomal abnormalities were produced which included gaps, breaks, bivalent and quadriradial configurations, ring chromosomes, dicentrics, abnormal metacentrics and submetacentrics, chromatic exchanges. Tetraploidy was rarely detected. The abnormalities were comparable in type to those seen in human VOD. At all dosages exchanges predominated over other types of abnormalities. Abnormalities were induced within 48 hours after fulvine administration at a threshold dosage of 0.02 mg/gm. Mitotic inhibition and liver damage were more marked in 12 weanling rats treated with doses of 0.08 mg/gm body weight of fulvine. The model for investigation of fulvine as a mutagenic agent was evaluated (AU)


Assuntos
Humanos , Criança , Adolescente , Ratos , Masculino , Feminino , Alcaloides de Pirrolizidina/efeitos adversos , Cromossomos Humanos/patologia , Cromossomos/análise , Testes de Mutagenicidade , Hepatopatia Veno-Oclusiva , Histologia , Fígado , Mitomicinas , Alcaloides de Pirrolizidina/farmacologia
3.
West Indian med. j ; 19(4): 258, Dec. 1970.
Artigo em Inglês | MedCarib | ID: med-6358

RESUMO

Some pyrrolizidine alkaloids have been shown to have nuclear toxicity in vitro. Fulvine was therefore investigated for its effect on mitotic chromosomes in the rat and in man. In vitro studies were performed on rat fibroblasts and human and rat peripheral blood cultures. In vivo studies were performed by injecting mature rats subcutaneously with 0.08mg/gm body weight in fulvine. Control animals received the identical dilutant. Blood cultures were established 24 and 48 hours, and 8 days later. Nine children with histologically proven veno-occlusive disease (VOD) and eight controls were studied by 48-hour blood cultures, for changes in chromosome number and morphology. The in vitro studies were negative. In vivo studies in rats showed marked mitotic depression at 24 and 48 hours. On the seventh day, breaks, gaps exchanges and rearrangements were seen in 24 percent of cells. VOD was found in 5 rats. Of the children, three sibs, and two others showed similar abnormalities marked in the former and less so in the latter. These findings are very suggestive of induction of chromosome abnormalities by fulvine or by VOD. The frequency of these changes may be related to dosage levels, intervals between ingestion and examination, or to familial reactions to the drug (AU)


Assuntos
Humanos , 21003 , Criança , Ratos , /efeitos adversos , Cromossomos/efeitos dos fármacos , Alcaloides de Pirrolizidina/efeitos adversos , Aberrações Cromossômicas/induzido quimicamente
4.
Br J Cancer ; 24(2): 266-76, June 1970.
Artigo em Inglês | MedCarib | ID: med-14573

RESUMO

Two cases are described, one with proven lymphosarcoma and doubtful autoimmune disease, and the second with the reverse situation, in which circulating abnormal mononuclear cells showed PHA responsiveness and an abnormal chromosomal constitution (clonal evolution). These findings are discussed in the light of previous cytogenic studies of lymphoreticular neoplasia and autoimmune disease and the relationship between these two conditions (Summary)


Assuntos
Humanos , Adulto , Pessoa de Meia-Idade , Doenças Autoimunes/fisiopatologia , Cromossomos/análise , Linfoma não Hodgkin , Citogenética/instrumentação , Autopsia
6.
West Indian med. j ; 18(4): 247, Dec. 1969.
Artigo em Inglês | MedCarib | ID: med-6390

RESUMO

Between April, 1964 and December, 1968, we have performed buccal smears and chromosome studies on a total of 46 patients with primary and secondary amenorrhoea and oligomenorrhoea. RESULTS: (1) Secondary amenorrhoea - 4 cases. All had normal chromosomes, all were over 62" in height, with normal build and sexual development. (2) Oligomenorrhoea - 10 cases. Five had moderate degrees of virilisation. One of these had chromosomal findings suggestive of XX/XO mosaicism. Five patients had normal secondary sex characteristics and had normal chromosomes. All 10 patients were 60" or more in height. (3) Primary Amenorrhoea 32 patients. These can be divided into 3 clinical groups. (a) Those with sexual infantilism - 21 cases. Of these, 10 showed sex chromosomal abnormalities. Six of these were less than 60" and hibited other features suggestive of gonadal dysgenesis. Of the 11 with normal chromosomes, 4 were under 57" in height and 3 of these were mentally retarded. (a) Those with sexual infantilism - 21 cases. Of these, 10 showed sex chromosomal abnormalities. Six of these were less than 60" and exhibited other features suggestive of gonadal dysgensis. Of the 11 with normal chromosomes, 4 were under 57" in height and 3 of these were mentally retarded. (b) Patients with virilisation - 7 cases. All these patients were small in size, around 60 to 63 inches in height, but were not dwarfed. Two were found to have congenital adrenal hyperplasia. The others have not all been fully investigated for this condition. (c) Normal secondary sex characteristics - 4 cases. Three had normal chromosomes. One patient had 46xx but a small line showed a small chromosomal fragment. Laparotomy revealed normal ovaries. Two other patients in this group had atresia of the vagina and cervix. CONCLUSION: 34 percent of patients with primary amenorrhoea have chromosmal abnormalities. Chromosome studies are, therefore, indicated in all such patients and probably some with oligomenorrhoea. All patients except those found to have congenital adrenal hyperplasia should have either full genital tract examinations or gonadal exploration, the former being indicated if there are normal secondary sex characteristics, and the latter if there is infantilism or virilisation not explained by congenital adrenal hyperplasia (AU)


Assuntos
Humanos , Feminino , Amenorreia , Cromossomos , Aberrações Cromossômicas , Oligomenorreia
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