Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Mais filtros

Filtros aplicados

Base de dados
Intervalo de ano de publicação
In. The University of the West Indies, Faculty of Medical Sciences. Faculty of Medical Sciences, Research Day. St. Augustine, Caribbean Medical Journal, March 21, 2019. .
Não convencional em Inglês | MedCarib | ID: biblio-1026460


Mycobacteria have, for a long time, been suspected to be causing severe disease in ornamental and farmed fish in Trinidad and Tobago (T&T), however, up to now, these mycobacteria species have not been identified and characterised. Many piscine mycobacteria species are also known to be zoonotic, potentially affecting human health. Objective: To identify and characterize the species of mycobacteria affecting fish (and possibly man) in T&T. Design and Methodology: Homogenised internal organs were collected from a total of 13 fish showing clinical signs consistent with mycobacterial infection. Samples were analysed using Ziehl-Neelsen (acid-fast) staining and real-time Polymerase Chain Reaction (rPCR). The species of mycobacteria were further characterised using conventional PCR targeting the 16s rRNA (564 bp), rpoB (396 bp) and sod (408 bp) genes. PCR products were sequenced and the sequences were compared with those from known and recently identified mycobacteria species through phylogenetic analysis. Results: Acid-fast non-branching bacilli were detected in all samples. All samples were also positive for Mycobacterium sp. by real-time PCR. Multi-gene phylogenetic analysis revealed the presence of two distinct species of mycobacteria. One aligned closely with Mycobacterium marinum, a well known pathogen affecting fish and man, and a second aligned closely with a species also known to affect both fish and humans, Mycobacterium stomatepiae. Conclusions: Phylogenetic analysis demonstrated the presence of two mycobacterium species in organs from fish showing clinical signs of Piscine Mycobacteriosis in T&T. Further work is needed to characterise these mycobacteria species and investigate their zoonotic potential.

Doenças dos Peixes , Mycobacterium , Trinidad e Tobago , Região do Caribe/etnologia
West Indian med. j ; 45(suppl. 2): 16-7, Apr. 1996.
Artigo em Inglês | MedCarib | ID: med-4651


Organisms of the Mycobacterium fortuitum complex are recognized, but uncommon, causes of pulmonary disease, primary cutaneous disease and a wide spectrum of nosocomial infections. M.fortuitum was isolated from 20 patients over a 15-month period, with an apparent clustering of isolates of M.fortuitum occurring from January to March 1994. The molecular epidemiology of this clustering was investigated using an arbitrary primer-polymerase chain reaction method (AP-PCR). Twenty-two isolates were studied and yielded 14 distinct profiles. Multiple isolates from a single patient yielded identical profiles. All of seven isolates of M.fortuitum recovered during the six-weeks period from January to March 1994 shared a common profile which was distinct from all other isolates, suggesting that a single strain was located from specimens from all seven patients. The source of the cluster of isolates of M.fortuitum is uncertain. We can find no epidemiological link upon which to suppose an episode of cross-infection within the hospital environment. Therefore it seems likely that contamination of the specimens during collection, transport or processing was responsible for the "pseudo-outbreak" of M.fortuitum observed in this study (AU)

Humanos , Mycobacterium/isolamento & purificação , Infecção Hospitalar/transmissão , Infecções por Mycobacterium
West Indian med. j ; 31(4): 198-204, Dec. 1982.
Artigo em Inglês | MedCarib | ID: med-11367


Nine hundred and eight Barbadian children aged 4 to 8 years were tested simultaneously by intradermal injection of 0.05 g standard tuberculin (PPD-S) and an extract of Mycobacterium intracellulare (Battey bacillus) (PPD-B). Of the total number of children tested, 21.8 percent reacted to both antigens. The reaction to PPD-S was significant in 2.6 percent and to PPD-B in 13.4 percent. In 5.9 percent of the children who reacted to one or both antigens the reactions were of equal size; the reaction to PPD-S was larger in 17.4 percent and to PPD-B in 76 percent. It is concluded that the infection atypical mycobateria is not uncommon in Barbados and that a large number of moderate and reactions to tuberculin are the result of atypical infection. There is evidence that atypical mycobaterial infection protects againsts infection with M. tuberculosis and vice versa. No tuberculous infection was found in the children under four years of age. In the 4 to 5, to 6 to 7 and 7 to 8-year age groups, the age specific infection rates were respectively 1.8 percent, 4.7 percent and 4.6 percent. It is concluded that the policy of administering BCG vaccine to children of 5 years of age should be continued. There is some indication that a number of cases of tuberculosis in Barbados are either not being diagnosed or not being reported (AU)

Criança , Humanos , Antígenos de Bactérias/imunologia , Mycobacterium/imunologia , Infecções por Mycobacterium/diagnóstico , Micobactérias não Tuberculosas/diagnóstico , Micobactérias não Tuberculosas/imunologia , Teste Tuberculínico/métodos , Tuberculose/diagnóstico , Testes Intradérmicos , Barbados
West Indian med. j ; 22(4): 193, Dec. 1973.
Artigo em Inglês | MedCarib | ID: med-6206


Tuberculosis morbidity in Barbados has fallen rapidly in recent years. This study was undertaken to discover whether the epidemiological situation has altered sufficiently to require a change in our policies on BCG-vaccination and case-finding. Tuberculin tests were carried out and read on 500 children aged about 5 years at 12 schools in three of the island's eleven parishes, using PPD 10 TU in 0.1 mil given intradermally. Only one natural reactor was found among the 500 children, though 15 others who had previously had BCG vaccination gave a positive response. 478 children showed no induration at all, and only 6 reactions fell in the range 1-8 mm, indicating that there is very little infection with atypical mycobacteria among the tested children. The results indicate that the transmission of tuberculosis in Barbados is at a very low level, comparable with that of the countries of Europe and North America. In the United States the low prevalence of tuberculin reactors among children has given rise to the concept of localised tuberculosis epidemics occurring round a single infectious case, and the object of control measures is to identify and stamp out these outbreaks. This is done by finding cases, especially among high risk groups, treating them promptly, and protecting tuberculin-positive and tuberculin-negative contacts with chemoprophy. The importance of the survey in planning immunization and control programmes will be considered for Barbados (AU)

Humanos , Criança , Tuberculose/transmissão , Barbados/epidemiologia , Morbidade , Mycobacterium , Tuberculose/epidemiologia , Tuberculose/imunologia