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West Indian med. j ; 45(Suppl 2): 37, Apr. 1996.
Artigo em Inglês | MedCarib | ID: med-4597


The presence of human papilloma virus (HPV) nucleotide sequences in paraffin sections of genital biopsies was examined by in-situ hybridization using non-isotopic, random primer biotin labeled probes representing HPV type 16. Biotin labeling of probes was performed using DNA labeling and commercially provided phenotype detection kit. Hybridization was performed under stringent conditions. The hybrids were detected by using Streptavidin and biotinylated alkaline phosphatase and visualized with an enzyme catalyzed chemiluminescent detection reaction. In-situ hybridization with biotinylated probes was a useful technique for the identification of HPV infection. The results were compared with the results obtained with dot blot hybridization probes. The background with biotinylated probes was very low. Using non radioactive robes the localization of hybrids at the cellular level was not better than dot blot labeled probes (AU)

Humanos , Papillomaviridae/isolamento & purificação , Sondas de DNA , Hibridização In Situ , Biópsia
Blood ; 75(2): 428-33, Jan. 15, 1990.
Artigo em Inglês | MedCarib | ID: med-10028


Human T-cell lymphotropic virus type I (HTLV-I) proviral integration status was examined by Southern blot analysis in peripheral blood mononuclear cell (PBMC) DNA from patients presenting a tropical spastic paraparesis (TSP) and serological evidence of HTLV-I infection. Surface phenotype and morphological aspects of PBMC were also studied. A polyclonal HTLV-I proviral integration was found in the PBMC of the 10 patients studied irrespective of their geographical origin (French West Indies, French Guiana, and Africa), the duration of their clincal illness, or the HTLV-I antibody titer. Furthermore, by dilution experiments and hypothesizing that only one copy of HTLV-I proviral DNA is present in one call, we estimated that this HTLV-I integration is present in 3 percent to 15 percent of their PBMC. All 10 TSP/HTLV-I patients studied had an average of 10 percent of thier lymphocytes abnormal, presening either a misshapen nucleus or an adult T-cell leukemia/lymphoma(ATL)-like feature. Moreover, an elevated CD4/CD8 ratio associated with the presence of activated T cells with a high level of DR expression was observed in most patients. The significant frequency of viral-positive PBMC and the important load of HTLV-I proviral DNA that we observed in TSP/HTLV-I patients might play an important role in the pathogenesis of this recently identified clinico-virological entity. (AU)

Humanos , Vírus Linfotrópico T Tipo 1 Humano/genética , Leucócitos Mononucleares/microbiologia , Paraparesia Espástica Tropical/microbiologia , Anticorpos Monoclonais , Antígenos CD , Southern Blotting , Células Clonais , Sondas de DNA , DNA Viral/análise , Guiana Francesa , Anticorpos Antideltaretrovirus/análise , Costa do Marfim , Martinica , Mapeamento por Restrição , Proteínas do Envelope Viral/genética , Índias Ocidentais , República Democrática do Congo
Diabetologia ; 31(12): 864-70, Dec. 1988.
Artigo em Inglês | MedCarib | ID: med-12510


Type 1 (insulin-dependent) diabetic patients and control subjects of Afro-Caribbean Negroid racial origin were investigated by serological HLA-DR-typing and restriction fragment length polymorphism analysis using DNA probes corresponding to the DQO, DQá and DRá chain genes. Combined analysis indicated that four DR antigens are positively associated with the condition in Negroid subjects - DR3, 4, 7 and w9. DR3 and 4 are also associated in Caucasians, but the relative risk for DR3 is lower in Negroid subjects. The DR7 association is specific for the Negroid race, and DRw9 is only weakly associated in Caucasoid subjects. Restriction fragment length polymorphism analysis demonstrated a DQá restriction pattern in Negroid subjects which is absent from Caucasoid subjects. This pattern was associated with DRw9 and a subset of DR7, and was markedly increased in frequency in diabetic patients compared with control subjects (48.7 percent vs 10.4 percent respectively; p<10 -4). In the absence of this pattern, DR7 showed no positive association. DR3 in Negroid subjects was associated with two distinct DQO-DQá patterns, only one of which was positively associated with diabetes. A DQá pattern, in linkage disequilibrium with different DR antigens in different races, conferred a consistent protective effect against the development of Type 1 diabetes. Trans-racial genetic analysis thus supports a primary role for DQ in susceptibility to Type 1 diabetes. (AU)

Humanos , /genética , Antígenos HLA-DQ/genética , /imunologia , Suscetibilidade a Doenças , Sondas de DNA , Reino Unido , Jamaica/etnologia , Valores de Referência , Polimorfismo de Fragmento de Restrição