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Antoine van Leeuwenhoek ; 74(4): 283-91, Nov. 1998.
Artigo em Inglês | MedCarib | ID: med-1331


Phylogenetic relationships of the yeast genus Lipomyces were studied using sequences from fragments of 5.8S rRNA gene and from internal transcribed spacer region ITS2 of 13 strains (7 type strains included) representing five species and subtaxa, and originating from different geographical locations (Japan, Trinidad, Nigeria, North America, Western Europe, Russia, South Africa, Mauritius). Parsimony and distance analyses were performed. Tree topology from the parsimony and distance analyses of the sequence confirmed the results of nDNA reassociation. Results segregate the 13 isolates of Lipomyces into five major clades.(Au)

Estudo Comparativo , DNA Ribossômico/genética , RNA Ribossômico 5,8S/genética , Saccharomycetales/classificação , Saccharomycetales/genética , Geografia , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Sequência de Bases
J Gen Virol ; 73(12): 3301-5, Dec. 1992.
Artigo em Inglês | MedCarib | ID: med-8509


Venezuelan equine encephalitis (VEE) is a mosquito-borne pathogen that has caused encephalitis in equine species and humans during sporadic outbreaks in the western hemisphere. The last, and most widespread, VEE outbreak occurred in South America, Central America, Mexico and the U.S.A. (Texas) during 1969 to 1972. We have cloned and sequenced the genome of a virulent VEE subtype I-AB virus, strain 71-180, isolated in Texas in 1971. Thirty four nucleotide differences were detected between the genome of 71-80 virus and that of subtype I-AB Trinidad donkey (TRD) virus isolated during the 1943 VEE epizootic in Trinidad. Fifteen nucleotide changes occurred in the non-structural genes, 16 in the structural genes and three in the 3' non-coding region. Only six of the nucleotide diferences resulted in amino acid substitutions: one change in each of non-structural proteins nsP1 and nsP3, two in the E2 envelope glycoprotein, one in the 6K popypeptide and one in the E1 envelope glycoprotein. The close genetic relationship between 71-180 virus and TRD virus, commonly used for production of formalin-inactivated VEE vaccines, suggests that incompletely inactivated virulent vaccine virus may have been the source of this and other VEE outbreaks. Use of formalized virulent virus was discontinued during the 1969 to 1972 panzootic. No VEE epizootics have been reported since the introduction of the live attenuated TC-83 vaccine virus (AU)

Humanos , 21003 , Vírus da Encefalite Equina Venezuelana/patogenicidade , Encefalite por Arbovirus/microbiologia , Vírus da Encefalite Equina do Oeste/patogenicidade , América do Norte , Homologia de Sequência do Ácido Nucleico , Sequência de Aminoácidos , América do Sul
J Gen Virol ; 69(7): 1695-710, July 1988.
Artigo em Inglês | MedCarib | ID: med-10044


We report the first complete nucleotide sequence of an adult T cell leukaemia virus/human T cell leukaemia virus type I (ATLV/HTLV) isolate from a British patient of Caribbean origin. Sequence comparisons of our proviral clone (HS-35) with other molecular clones are shown. We note the strong sequence conservation between isolates of Caribbean and Japanese origin (2.3 percent divergence), but demonstrate the higher homologies existing between isolates originating from similar geographical areas (approximately 1 percent divergence). Implications for the origin, evolution and dissemination of the ATLV/HTLV-I subgroup are discussed. Analysis of defective proviral clones isolated from the same genomic library is also reported,and suggests a pattern of proviral sequence deletions during the biogenesis of defective proviruses. (AU)

Humanos , Genes Virais , Infecções por Deltaretrovirus/microbiologia , Deltaretrovirus/genética , Sequência de Aminoácidos , Sequência de Bases , Inglaterra , Infecções por Deltaretrovirus/etnologia , Deltaretrovirus/classificação , Deltaretrovirus/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Provírus/genética , Homologia de Sequência do Ácido Nucleico , Proteínas do Envelope Viral/genética , Índias Ocidentais