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1.
Artigo em Inglês | MedCarib | ID: med-17701

RESUMO

Water reservoirs formed by the leaf axils of bromeliads are a highly derived system for nutrient and water capture that also house a diverse fauna of invertebrate specialists. Here we investigate the origin and specificity of bromeliad-associated insects using Copelatinae diving beetles (Dytiscidae). This group is widely distributed in small water bodies throughout tropical forests, but a subset of species encountered in bromeliad tanks is strictly specialized to this habitat. An extensive molecular phylogenetic analysis of Neotropical Copelatinae places these bromeliadicolous species in at least three clades nested within other Copelatus. One lineage is morphologically distinct, and its origin was estimated to reach back to 12-23 million years ago, comparable to the age of the tank habitat itself. Species of this clade in the Atlantic rainforest of southern Brazil and mountain ranges of northern Venezuela and Trinidad show marked phylogeographical structure with up to 8% mtDNA divergence, possibly indicating allopatric speciation. The other two invasions of bromeliad water tanks are more recent, and haplotype distributions within species are best explained by recent expansion into newly formed habitat. Hence, bromeliad tanks create a second stratum of aquatic freshwater habitat independent of that on the ground but affected by parallel processes of species and population diversification at various temporal scales, possibly reflecting the paleoclimatic history of neotropical forests.


Assuntos
Animais , Besouros/fisiologia , Biodiversidade , Bromelia/parasitologia , Ecossistema , Evolução Molecular , Dados de Sequência Molecular , Filogenia , Simbiose , Árvores/parasitologia , Clima Tropical , Trinidad e Tobago
2.
Journal of virology ; 79(6): 3807-3821, Mar. 2005. graf, tab, ilus
Artigo em Inglês | MedCarib | ID: med-17658

RESUMO

The 3' noncoding region (3' NCR) of flaviviruses contains secondary and tertiary structures essential for virus replication. Previous studies of yellow fever virus (YFV) and dengue virus have found that modifications to the 3' NCR are sometimes associated with attenuation in vertebrate and/or mosquito hosts. The 3' NCRs of 117 isolates of South American YFV have been examined, and major deletions and/or duplications of conserved RNA structures have been identified in several wild-type isolates. Nineteen isolates (designated YF-XL isolates) from Brazil, Trinidad, and Venezuela, dating from 1973 to 2001, exhibited a 216-nucleotide (nt) duplication, yielding a tandem repeat of conserved hairpin, stem-loop, dumbbell, and pseudoknot structures. YF-XL isolates were found exclusively within one subclade of South American genotype I YFV. One Brazilian isolate exhibited, in addition to the 216-nt duplication, a deletion of a 40-nt repeated hairpin (RYF) motif (YF-XL-DeltaRYF). To investigate the biological significance of these 3' NCR rearrangements, YF-XL-DeltaRYF and YF-XL isolates, as well as other South American YFV isolates, were evaluated for three phenotypes: growth kinetics in cell culture, neuroinvasiveness in suckling mice, and ability to replicate and produce disseminated infections in Aedes aegypti mosquitoes. YF-XL-DeltaRYF and YF-XL isolates showed growth kinetics and neuroinvasive characteristics comparable to those of typical South American YFV isolates, and mosquito infectivity trials demonstrated that both types of 3' NCR variants were capable of replication and dissemination in a laboratory-adapted colony of A. aegypti.


Assuntos
Camundongos , Animais , Humanos , Research Support, Non-U.S. Gov't , Research Support, U.S. Gov't, P.H.S. , Aedes/virologia , Sequência de Bases , Células Cultivadas , Variação Genética , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Filogenia , RNA não Traduzido/química , RNA não Traduzido/genética , RNA não Traduzido/fisiologia , RNA Viral/química , RNA Viral/genética , RNA Viral/fisiologia , Vírus da Febre Amarela/classificação , Vírus da Febre Amarela/crescimento & desenvolvimento , Vírus da Febre Amarela/genética , Vírus da Febre Amarela/isolamento & purificação , Vírus da Febre Amarela/patogenicidade , Trinidad e Tobago , Brasil , Venezuela
3.
Genetics ; 155(3): 1313-1320, Jul. 2000. tab, graf
Artigo em Inglês | MedCarib | ID: med-17772

RESUMO

The standard slipped-strand mispairing (SSM) model for the formation of variable number tandem repeats (VNTRs) proposes that a few tandem repeats, produced by chance mutations, provide the "raw material" for VNTR expansion. However, this model is unlikely to explain the formation of VNTRs with long motifs (e.g., minisatellites), because the likelihood of a tandem repeat forming by chance decreases rapidly as the length of the repeat motif increases. Phylogenetic reconstruction of the birth of a mitochondrial (mt) DNA minisatellite in guppies suggests that VNTRs with long motifs can form as a consequence of SSM at noncontiguous repeats. VNTRs formed in this manner have motifs longer than the noncontiguous repeat originally formed by chance and are flanked by one unit of the original, noncontiguous repeat. SSM at noncontiguous repeats can therefore explain the birth of VNTRs with long motifs and the "imperfect" or "short direct" repeats frequently observed adjacent to both mtDNA and nuclear VNTRs.


Assuntos
Animais , Research Support, Non-U.S. Gov't , Pareamento Incorreto de Bases/genética , Sequência de Bases , DNA Mitocondrial/análise , DNA Mitocondrial/genética , Genética Populacional , Repetições Minissatélites/genética , Mitocôndrias/genética , Modelos Genéticos , Dados de Sequência Molecular , Mutação , Filogenia , Poecilia/genética , Análise de Sequência de DNA , Trinidad e Tobago
4.
J Med Virol ; 59(2): 245-55, Oct. 1999.
Artigo em Inglês | MedCarib | ID: med-1311

RESUMO

Human T-cell lymphotropic virus type I (HTLV-I) is associated with adult T-cell leukemia (ATL) and tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM). Other inflammatory disorders may occur in HTLV-I-infected patients, such as sicca syndrome resembling Sjogren's syndrome. The sicca syndrome may be the unique clinical manifestation of HTLV-I infection, but is associated frequently with TSP/HAM, which could suggest that sicca syndrome might be an early event in disease progression to TSP/HAM in some cases. We investigated whether peculiar pX and LTR mutations could be related to sicca syndrome, or might argue the existence of clinical progression to TSP/HAM. pX, especially pX(I), pX(II), and pX(IV) ORFs corresponding to Tax cytotoxic T-lymphocyte epitopes, and LTR regions from Caribbean patients who have sicca sydrome with or without TSP/HAM, ATL patients, and healthy carriers were sequenced. The sequences were aligned and compared with ATK-1 prototype and published sequences. LTR sequences exhibited 1.5-2.4 percent of divergence with ATK-1. pX-sequenced regions showed a lower homology within p12(I) encoding sequences. Only few mutations were found within functionally important regions, but were not associated specifically with the clinical status. Finally, no existence of clinical progression to TSP/HAM were found. It would be of interest to study the clinical evolution of HTLV-I-sicca syndrome in patients and to determine HTLV-I sequences from peripheral blood and salivary glands at different stages. Copyright 1999 Wiley-Liss, Inc.(Au)


Assuntos
Idoso , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Vírus Linfotrópico T Tipo 1 Humano/genética , Infecções por HTLV-I/virologia , Paraparesia Espástica Tropical/diagnóstico , Análise de Sequência de DNA/métodos , Síndrome de Sjogren/virologia , Sequências Repetidas Terminais/genética , Idoso de 80 Anos ou mais , Sequência de Bases , Região do Caribe , Progressão da Doença , Genoma Viral , Dados de Sequência Molecular , Mutação , Fases de Leitura Aberta/genética , Paraparesia Espástica Tropical/virologia , Alinhamento de Sequência
5.
Am J Trop Med Hyg ; 60(4): 630-4, Apr. 1999.
Artigo em Inglês | MedCarib | ID: med-1351

RESUMO

Phylogenetic analysis of 20 strains of Venezuelan equine encephalitis (VEE) virus subtype IE isolated from 1961 to 1996 in Mexico and throughout Central America showed that VEE virus subtype IE was monophyletic with respect to other VEE virus subtypes. Nonetheless, there were at least three distinct geographically separated VEE virus IE genotypes: northwestern Panama, Pacific coast (Mexico/Guatemala), and Gulf/Caribbean coast (Mexico/Belize). Strains from the Caribbean coast of Guatemala, Honduras, and Nicaragua may cluster with the Gulf/Caribbean genotype, but additional isolates from the region between Guatemala and Panama will be required to firmly establish their phylogenetic position. Viruses associated with two separate equine epizootics in Mexico in the 1990s were phlogenticaly related to nonepizootic viruses from neighbouring Guatemala and may represent the emergence or re-emergence of equine-virulent VEE virus subtype IE in Middle America.(AU)


Assuntos
Adulto , Criança , 21003 , Humanos , Recém-Nascido , Vírus da Encefalite Equina Venezuelana/classificação , Vírus da Encefalite Equina Venezuelana/genética , Encefalomielite Equina Venezuelana/veterinária , Doenças dos Cavalos/virologia , Sequência de Aminoácidos , América Central , Vírus da Encefalite Equina Venezuelana/isolamento & purificação , Encefalomielite Equina Venezuelana/virologia , Cavalos , México , Dados de Sequência Molecular , Filogenia , Precursores de Proteínas/química , Precursores de Proteínas/genética , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/genética
6.
Biochem Biophys Res Commun ; 257(2): 300-5, Apr. 13, 1999.
Artigo em Inglês | MedCarib | ID: med-1360

RESUMO

Heartwater is an economically important disease of ruminants caused by the tick-transmitted rickettsia Cowdria ruminantium. The disease is present in Africa and the Caribbean and there is a risk of spread to the Americas, particularly because of a clinically asymptomatic carrier state in infected livestock and imported wild animals. The causative agent is closely related taxonomically to the human and animal pathogens Ehrlichia chaffeensis and Ehrlichia canis. A dominant immune response of infected animals or people is directed against variable outer membrane proteins of these agents known, in E. chaffeensis and E. canis, to be encoded by polymorphic multigene families. We demonstrate, by sequence analysis, the map1 encoding the major outer membrane protein of C. ruminantium is also encoded by a polymorphic multigene family. Two members of the gene family are located in tandem in the genome. The upstream member, orf2, is conserved, encoding only 2 amino acid substitution among six different rickettsial strains from diverse locations in Africa and the Caribbean. In contrast, the downstream member, map1, contains variable and conserved regions between strains. Interestingly, orf2 is more closely related in sequence to omplb of E. chaffeensis than to map1 of C. ruminantium. The regions that differ among orf2, map1, and omp1b correspond to previously identified variable sequences in outer membrane protein genes of E. chaffeensis and E. canis. These data suggest that diversity in these outer membrane proteins may arise by recombination among gene family members and offer a potential mechanism for persistence of infection in carrier animals.(AU)


Assuntos
Estudo Comparativo , Proteínas da Membrana Bacteriana Externa/genética , Sequência Conservada , Ehrlichia ruminantium/genética , Família Multigênica/genética , Variação Genética , África , Sequência de Aminoácidos , Região do Caribe , Bases de Dados Factuais , Ehrlichia chaffeensis/genética , Genoma Bacteriano , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Reação em Cadeia da Polimerase , Polimorfismo Genético , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos
7.
Am J Trop Med Hyg ; 60(3): 364-76, Mar. 1999.
Artigo em Inglês | MedCarib | ID: med-1345

RESUMO

A genetic and morphologic survey of Anopheles darlingi populations collected from seven countries in Central and South America was performed to clarify the taxonomic status of this major malaria vector species in the Americas. Population genetics was based on three techniques including isozyme, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), and internal transcribed spacer 2 (ITS2) markers. The results of the isozyme analysis indicated moderate differences in the allele frequencies of three putative loci (glutamate oxalaoacetate transaminase-1, isocitrate dehydrogenase-1, and phosphoglucomutase) of the 31 analyzed. No fixed electromorphic differences separated the populations of An. darlingi, which showed little genetic divergence (Nei distances = 0.976-0.995). Fragments produced by RAPD-PCR demonstrated evidence of geographic partitioning and showed that all populations were separated by small genetic distances as measured with the 1 - S distance matrix. The ITS2 sequences for all samples were identical except for four individuals from Belize that differed by a three-base deletion (CCC). The morphologic study demonstrated that the Euclidean distances ranged from 0.02 to 0.14, with the highest value observed between populations from Belize and Bolivia. Based on these analyses, all the An. darlingi populations examined demonstrated a genetic similarity that is consistent with the existence of a single species and suggest that gene flow is occurring throughout the species' geographic range.(Au)


Assuntos
21003 , Feminino , Anopheles/classificação , Insetos Vetores/classificação , Malária/transmissão , Sequência de Bases , Belize , Eletroforese em Gel de Amido/veterinária , Anopheles/enzimologia , Anopheles/genética , Insetos Vetores/enzimologia , Insetos Vetores/genética , Isocitrato Desidrogenase/química , Isoenzimas/análise , Dados de Sequência Molecular , Fosfoglucomutase/química , Filogenia , Reação em Cadeia da Polimerase/veterinária , Técnica de Amplificação ao Acaso de DNA Polimórfico/veterinária , Análise de Sequência de DNA , América do Sul
8.
Antoine van Leeuwenhoek ; 74(4): 283-91, Nov. 1998.
Artigo em Inglês | MedCarib | ID: med-1331

RESUMO

Phylogenetic relationships of the yeast genus Lipomyces were studied using sequences from fragments of 5.8S rRNA gene and from internal transcribed spacer region ITS2 of 13 strains (7 type strains included) representing five species and subtaxa, and originating from different geographical locations (Japan, Trinidad, Nigeria, North America, Western Europe, Russia, South Africa, Mauritius). Parsimony and distance analyses were performed. Tree topology from the parsimony and distance analyses of the sequence confirmed the results of nDNA reassociation. Results segregate the 13 isolates of Lipomyces into five major clades.(Au)


Assuntos
Estudo Comparativo , DNA Ribossômico/genética , RNA Ribossômico 5,8S/genética , Saccharomycetales/classificação , Saccharomycetales/genética , Geografia , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Sequência de Bases
9.
J Hypertens ; 14(3): 315-21, Mar. 1996.
Artigo em Inglês | MedCarib | ID: med-2993

RESUMO

OBJECTIVE: To examine the association between blood pressure, angiotensinogen levels, angiotensin converting enzyme activity and polymorphisms of the angiotensinogen and angiotensin converting enzyme genes in a population-based sample. METHOD: Five hundred participants were recruited in a house-to-house survey of three communities in metropolitan areas of Kingston and St. Andrew, in Jamaica. Demographic data, anthropometric and blood pressure measurements were obtained for each participant during a brief clinic visit. Circulating levels of angiotensinogen and angiotensin converting enzyme genes were determined. RESULTS: A weak association between angiotensinogen level, angiotensin converting enzyme activity and blood pressure was identified in this population, but substantial joint effect of angiotensin converting enzyme activity and agiotensinogen level on blood pressure was apparent. Variants of the angiotensinogen gene had inconsistent effects on blood pressure and on the risk of hypertension. Angiotensinogen level and angiotensin converting enzyme activity were significantly related to several measures of obesity, including body mass index, waist circumference and skin fold thickness. CONCLUSION: The angiotensinogen and angiotensin converting enzyme genetic variants which were studied appear to have only a modest relationship with blood pressure and associated anthropometric risk factors among blacks. (AU)


Assuntos
Adulto , Humanos , Feminino , Masculino , Pessoa de Meia-Idade , Idoso , Angiotensina II , Angiotensinogênio/sangue , Pressão Arterial , Sequência de Bases , Hipertensão/genética , Dados de Sequência Molecular , Peptidil Dipeptidase A/sangue , Jamaica
10.
Arthritis Rheum ; 38(5): 690-8, May 1995.
Artigo em Inglês | MedCarib | ID: med-2077

RESUMO

OBJECTIVE: To investigate a possible association between human T cell leukemia/lymphoma virus type I (HTLV-I) and polymyositis (PM). METHODS: Sera and muscle biopsy samples from 9 Jamaican PM patients were compared with specimens from American HTLV-I positive PM patients and normal controls. Sera were evaluated for HTLV antibodies by enzyme-linked immunosorbent assay and Western blot. The biopsy samples were analyzed for HTLV-I/II DNA by polymerase chain reaction and were also immunohistochemically stained for HTLV gp46 envelope protein. RESULTS: Seven of the 8 Jamaican PM patients from whom sera were available were HTLV-I seropositive. The muscle biopsies of all 9 Jamaican patients demonstrated severe lymphocytic infiltration, cellular degeneration, myofiber atrophy, and fibrosis. Each muscle biopsy specimen contained HTLV-I DNA. Two of 6 samples demonstrated intense staining for HTLV-I gp46 in many of the invading mononuclear cells and weak staining for HTLV-I gp46 in many of the other specimens were weakly positive for gp46 in rare mononuclear cells. All controls specimens were negative for the presence of HTLV-I DNA and protein. CONClUSION: HTLV-I is associated with an inflammatory muscle disease characterized by direct invasion of the affected muscle by HTLV-I-infected mononuclear cells.(AU)


Assuntos
Adulto , Pessoa de Meia-Idade , DNA Viral/isolamento & purificação , Produtos do Gene env/análise , Anticorpos Anti-HTLV-I/sangue , Polimiosite/virologia , Proteínas Oncogênicas de Retroviridae/análise , Sequência de Bases , Biópsia , Dados de Sequência Molecular , Músculos/química , Músculos/patologia , Reação em Cadeia da Polimerase , Polimiosite/sangue , Polimiosite/imunologia , Polimiosite/patologia
11.
J Gen Virol ; 75(9): 2457-62, Sept. 1994.
Artigo em Inglês | MedCarib | ID: med-5903

RESUMO

The naturally occurring sequence variation of human papillomavirus type 16 (HPV-16) was analysed by direct sequence analysis of the PCR products of the long control region (LCR), the E5 and E7 open reading frames (OFRs), a segment of the L2 ORF overlapping the early viral poly(A) signal and a small segment of the L1 ORF or clinical isolates from Barbados and The Netherlands. Despite the widely different geographical and ethnic origin of the two groups of specimens, sequence analysis revealed relatively few mutational differences. Analysis of the LCR and the E5 ORF appeared to be the minimum requirement for the correct positioning of these variants in the HPV-16 phylogenetic tree. Most of the Barbadian variants appeared to be located at a unique position in the HPV-16 phylogenetic tree, at the internal branch close to the point where the European and Asian branches diverge. In contrast, most of the Dutch samples were located on the European branch. (AU)


Assuntos
Humanos , Filogenia , Reação em Cadeia da Polimerase/métodos , /genética , /genética , /isolamento & purificação , Ásia , Barbados , Sequência de Bases , DNA , Grupos Étnicos , Europa (Continente) , Dados de Sequência Molecular , Mutação , Países Baixos , Proteínas Oncogênicas Virais/genética , Estudo Comparativo
12.
J Infect Dis ; 165(2): 268-72, Feb. 1992.
Artigo em Inglês | MedCarib | ID: med-15940

RESUMO

A recombinant protein of the human T cell lymphotropic virus type I (HTLV-I) gp46 outer membrane envelope, MTA-4 (residues 129-203), reacted by Western blot with sera from HTLV-I-infected individuals from the United States and Jamaica but not with 24 (10 percent) of 242 Japanese sera. A related gp46 recombinant protein, MTA-1 (residues 162-209), reacted with all 58 sera from HTLV-I-infected US and Jamaican individuals and 238 of 242 sera from infected Japanese (combined sensitivity of 99 percent). Neither recombinant showed reactivity to sera from HTLV-II-infected individuals or uninfected controls. The reactivity of recombinant proteins containing the region of HTLV-II gp46 analogous to MTA-1 was also evaluated by Western blot: GH2-K15 (residues 157-205) and GH2-K55 (residues 162-205) reacted with 88 (98 percent) and 89 (99 percent), respectively, of 90 sera from HTLV-II-infected individuals but not with sera from HTLV-I-infected individuals or uninfected controls. These recombinant proteins should permit the development of assays to unambiguously confirm and differentiate HTLV-I and HTLV-II infections. (AU)


Assuntos
Humanos , Anticorpos Antideltaretrovirus/biossíntese , Antígenos HTLV-I/imunologia , Infecções por HTLV-I/diagnóstico , Antígenos HTLV-II/imunologia , Infecções por HTLV-II/diagnóstico , Sequência de Aminoácidos , Anticorpos Monoclonais/diagnóstico , Epitopos/imunologia , Western Blotting , Diagnóstico Diferencial , Antígenos HTLV-I , Antígenos HTLV-II , Jamaica , Japão , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Proteínas Recombinantes/imunologia , Estados Unidos
13.
Diabetes ; 40(6): 748-53, June 1991.
Artigo em Inglês | MedCarib | ID: med-12560

RESUMO

Transracial analysis provides a method of distinguishing primary associations between insulin-dependent diabetes mellitus (IDDM) and HLA class II alleles from those secondary to linkage disequilibrium. Blacks show DR-DQ relationships that are different from other races and are a useful group in which to investigate HLA-D region associations with IDDM. In this study, the frequencies of HLA-DQA1 and -DQB1 alleles in Afro-Caribbean IDDM and control subjects were compared. Alleles were identified with sequence-specific oligonucleotide probing. The DQA1 allele A3 was positively associated with IDDM (relative risk[RR] = 25.3, corrected P [Pc]<7.0 x 10 -6). THe DQB1 alleles DQw2 and DQw8 were also positively associated (RR = 4.7, Pc<6.5 x 10 -3 and RR = 12.3,Pc = 3.4 x 10 -3, respectively). The A1.2 and DQw6 alleles were negatively associated (RR = 0.16, Pc<3.5 x 10 -3 and RR = 0.15, Pc = 2.4 x 10 -2, respectively). These findings were compared to data from other races. The positive associations with A3 and DQw2 are consistent with all racial groups investigated. The negative association with DQw6 is present in all racial groups in which it is a common allele. These findings suggest that DQ alleles, and hence DQ molecules, may directly affect predisposition to IDDM. (AU)


Assuntos
Humanos , Alelos , /genética , Antígenos HLA-DQ/genética , Sequência de Bases , /imunologia , Suscetibilidade a Doenças/genética , Frequência do Gene , Reino Unido , Jamaica/etnologia , Dados de Sequência Molecular , Sondas de Oligonucleotídeos , Valores de Referência
14.
BMJ ; 300(3): 300-4, Feb. 3, 1990.
Artigo em Inglês | MedCarib | ID: med-14849

RESUMO

OBJECTIVE -- To compare the prevalence of antibody to and proviral DNA of the retrovirus HTLV-I in relatives of 11 British patients with tropical spastic paraparesis who migrated from Jamaica before they developed symptoms, and to examine factors possibly related to transmission of HTLV-I. DESIGN -- Migrant family study. Antibody state was determined by several methods and confirmed by western blotting; the polymerase chain reaction was used to detect proviral DNA. SETTING -- Britain and Jamaica. SUBJECTS -- All available first degree relatives: those born and still resident in Jamaica (group 1); those born in Jamaica who migrated to Britian (group 2); and index patients' children who were born and resident in Britian (group 3). All had been breast fed and none had had blood transfusions. RESULTS -- Of the 66 living relatives, 60 were traced. Seroprevalence among those born in Jamaica (irrespective of current residence) was 22 percent (10/46; 95 percent confidence limits 9 to 34 percent) compared with zero among British born offspring (0/14) and was higher in group 2 at 33 percent (7/21; 12 to 55 percent) than in group 1 at greatest mean age.) Proviral DNA was not detected in any subject negative for HTLV-I antibody, making prolonged viral incubation in those negative for the antibody unlikely. CONCLUSION -- In this sample factors related to place of birth and early residence were more important in transmission of HTLV-I than naternal or age effects. In areas with a low to moderate prevalence policies of preventing mothers who are carriers of the virus from breast feeding would be premature (AU)


Assuntos
Humanos , Pessoa de Meia-Idade , Masculino , Feminino , Infecções por Deltaretrovirus/epidemiologia , Paraparesia Espástica Tropical/epidemiologia , Sequência de Bases , Portador Sadio , Estudo Comparativo , DNA Viral/análise , Reino Unido/epidemiologia , Anticorpos Anti-HTLV-I/análise , Infecções por HTLV-I/genética , Jamaica/epidemiologia , Dados de Sequência Molecular , Paraparesia Espástica Tropical/transmissão , Linhagem , Reação em Cadeia da Polimerase , Prevalência , Fatores de Tempo
15.
J Gen Virol ; 69(7): 1695-710, July 1988.
Artigo em Inglês | MedCarib | ID: med-10044

RESUMO

We report the first complete nucleotide sequence of an adult T cell leukaemia virus/human T cell leukaemia virus type I (ATLV/HTLV) isolate from a British patient of Caribbean origin. Sequence comparisons of our proviral clone (HS-35) with other molecular clones are shown. We note the strong sequence conservation between isolates of Caribbean and Japanese origin (2.3 percent divergence), but demonstrate the higher homologies existing between isolates originating from similar geographical areas (approximately 1 percent divergence). Implications for the origin, evolution and dissemination of the ATLV/HTLV-I subgroup are discussed. Analysis of defective proviral clones isolated from the same genomic library is also reported,and suggests a pattern of proviral sequence deletions during the biogenesis of defective proviruses. (AU)


Assuntos
Humanos , Genes Virais , Infecções por Deltaretrovirus/microbiologia , Deltaretrovirus/genética , Sequência de Aminoácidos , Sequência de Bases , Inglaterra , Infecções por Deltaretrovirus/etnologia , Deltaretrovirus/classificação , Deltaretrovirus/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Provírus/genética , Homologia de Sequência do Ácido Nucleico , Proteínas do Envelope Viral/genética , Índias Ocidentais
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