An overview of yeast probiotics as cancer biotherapeutics: possible clinical application in colorectal cancer.

The previous reports have established a strong link between diet, lifestyle, and gut microbiota population with the onset of the colorectal cancer (CRC). Administration of probiotics has become a particular interest in prevention and treatment of CRC. As potential dietary complements, probiotics might be able to lower the risk of CRC and manage the safety of traditional cancer therapies such as surgery, radiation therapy, and chemotherapy. This review investigates the promising effects of probiotics as biotherapeutics, with due attention to possible clinical application of yeast probiotics in prevention and treatment of CRC. In addition, various underlying anti-cancer mechanisms are covered here based on scientific evidence and findings from numerous experimental studies. Application of probiotics as biotherapeutics in CRC, however, needs to be approved by human clinical trials. It is of prime concern, to find potential probiotic strains, effective doses for administrations and regimes, and molecular mechanisms involved in prevention and treatment.

Potential anticancer effects of cell wall protein fractions from Lactobacillus paracasei on human intestinal Caco-2 cell line.

Consumption of probiotics has an important role in colorectal cancer prevention. In this study, we aimed to explore that the cell wall protein fractions from Lactobacillus paracasei could induce apoptosis on Caco-2 cell line. The cell wall proteins from L. paracasei were fractionated by gel filtration chromatography (F1, F2 and F3) and characterized by polyacrylamide gel electrophoresis (SDS-PAGE). The anticancer properties were evaluated using MTT assay and Annexin V-FITC/PI staining. Administration of L. paracasei increased a significant concentration- and time-dependent anti-proliferative effect on Caco-2 cell line, determined by cell viability assays. However, a dramatic decrease in cell viability of Caco-2 cells was observed at the concentration of 100 µg ml-1 of F1 L. paracasei for 72 h (58% cell viability, P < 0·05) The results showed that F1 L. paracasei could induce apoptosis in Caco-2 cancer cell line by increased in annexin V and propidium iodide staining for 72 h (up to 90·6%, P < 0·001). These results indicated the importance of the anticancer effects of cell wall protein fractions of L. paracasei in human colon carcinoma Caco-2 cell line. Thus, cell wall protein fractions of L. paracasei can be a potential chemotherapeutic agent against Caco-2 cell lines. SIGNIFICANCE AND IMPACT OF THE STUDY: Significance and Impact of the Study: Our findings revealed that the newly identified cell wall protein fractions from probiotic Lactobacillus paracasei inhibit the cell growth of human colon carcinoma cell line (Caco-2), and the results indicated that the cell wall proteins from L. paracasei can be a potential chemotherapeutic agent against Caco-2 cell lines.

Ultrasonic/sonochemical synthesis and evaluation of nanostructured oil in water emulsions for topical delivery of protein drugs.

Delivery of drugs and active agents to human skin by formulation containing nanosystems have shown remarkable advance in recent nanotechnology research. The aim of current investigation is to study protein drug, extracted from medicinal leech tissue and evaluate an isotropic and kinetically stable nanoemulsion formulation, with least surfactant and co-surfactant concentrations also with optimal solubility and stability, for topical delivery. It is a fact that physical properties of oil phase has an impact on nanoemulsion formation and stabilization. In this research, various factors, such as oil types (olive oil and sesame oil) and oil content for their effects on particle size and stability of protein nanoemulsion were first investigated. Secondly, optimized formulation of protein nanoemulsion was characterized by droplet size and zeta potential analysis, Transmission electron microscopy (TEM), viscosity, pH, and refractive index. Thirdly, stability studies were done to select the best formulation. The results of our experiments showed that an increase in the concentration of olive oil and sesame oil led to a nanoemulsion with smaller size of droplets and with higher stability, respectively. However; slight variations in droplet size were observed in case of nanoemulsion with olive oil. As a result of various experiments, a Nanoemulsion with 25% olive oil was selected as optimized formulation owing to its much smaller droplet size (143.1 nm), lower polydispersity index, higher zeta potential (-33.3 mV). No considerable changes in droplet size, viscosity and pH occurred during a 30-day storage period at 4 °C. This procedure also suggested that our selected nanoemulsion was physically stable. Additionally, TEM revealed that particles were morphologically spherical. In conclusion, our analyses of the experiments proved the fact that nanoemulsions are promising novel formulations for protein drugs and can greatly enhance stability of protein drugs.