<i>In vitro</i> Characterization of the Essential Oil Extracted from <i>Lavandula dentata</i> and its Application in the Field of Biotherapy.

&lt;b&gt;Background and Objective:&lt;/b&gt; &lt;i&gt;Lavandula dentata &lt;/i&gt;essential oil holds promise as a valuable natural resource with diverse therapeutic potential. The main objective of this study was to investigate the bioactivities of &lt;i&gt;Lavandula dentata &lt;/i&gt;essential oil, specifically its antimicrobial, analgesic, anti-diabetic and anticoagulant properties. &lt;b&gt;Materials and Methods:&lt;/b&gt; The Algerian medicinal plant &lt;i&gt;Lavandula dentata &lt;/i&gt;EO was collected from Tipaza City (Algeria). The hydro-distillation method was used to get yield of essential oil. The GC/MS analysis was done to identify the bioactive compound of &lt;i&gt;Lavandula dentata&lt;/i&gt; EO. The antimicrobial activity of&lt;i&gt; L. dentata &lt;/i&gt;essential oil (EO) was assessed using the disc diffusion method against eight different microorganisms. The antidiabetic and anticoagulant activity was also studied. &lt;b&gt;Results:&lt;/b&gt; The hydrodistillation method yielded approximately 0.4% of essential oil. The GC/MS results showed that &lt;i&gt;L. dentata&lt;/i&gt; EO contains a total of 18 elements and the main constituents were 1.8-cineole (41.48%) and β-pinene (33.43%). The EO exhibited a robust inhibitory effect on the growth of all tested microorganisms, with inhibitory diameters ranging from 16.6 to 38.5 mm. &lt;i&gt;Lavandula dentata&lt;/i&gt; EO presented anti-diabetic activity by inhibiting the α-amylase enzyme, with an IC&lt;sub&gt;50&lt;/sub&gt; value of approximately 135.08±0.25 μg mL&lt;sup&gt;–1&lt;/sup&gt;, demonstrating significant anti-diabetic activity and anti-coagulant activity. &lt;b&gt;Conclusion:&lt;/b&gt; &lt;i&gt;Lavandula dentata&lt;/i&gt; EO processes great antimicrobial, analgesic, anti- diabetic and anticoagulant properties. Further investigations into its mechanisms of action and safety profile are warranted to fully exploit its therapeutic potential.

In vitro antibacterial activity and phytochemical analysis of White henbane treated by phytohormones.

This study reported the effect of interaction between cytokinins and auxins to enhance accumulation of alkaloids in White henbane (Hyoscyamus albus L.). Plants of this specie were grown under controlled conditions and treated with plant-hormones: Auxins by: 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and 3-Indole Acetic Acid (IAA), Cytokinins by: Kinetin (K) and Benzyl Amino Purine (BAP), at 0-10 and 20 mg L(-1) rates isolated and interacted. The results showed that treatment of 2, 4-D and K at the highest applied rates 20 mg L(-1) increased the accumulation threefold rate estimated to 2.321% in the root plant part and 1.702% in the aerial plant part with the same plant-hormones but dosage of (20x10 mg L(-1)) in order. The TLC for alkaloid extracts shows that H. albus L. contains 6 alkaloids. In this study, it was concluded that the treatment with interaction of (Kx2,4-D) (20x20 mg L(-1)) gives the highest percent of alkaloids in the root and shoot parts compared to plant-hormones separated. The in vitro antibacterial activity was determined on microorganisms: Pseudomonas stutzeri, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. And performed by disc diffusion assay. Respectively, ethanol extracts showed no inhibitory effect on the microorganisms, however alkaloid extracts of H. albus L. of the same treatments with plant-hormones of shoot and root parts showed antibacterial activity against microorganisms that were tested. The results obtained in the present study suggest that alkaloid of H. albus L. can be used in treating diseases caused by the test organisms.