RESUMEN
The aim of the study was to investigate the time-dependent increase in the knee extensors' isometric strength as a response to voluntary, unilateral, isometric knee extension exercise (UIKEE). To do so, a systematic review was carried out to obtain data for a Bayesian longitudinal model-based meta-analysis (BLMBMA). For the systematic review, PubMed, Web of Science, SCOPUS, Chochrane Library were used as databases. The systematic review included only studies that reported on healthy, young individuals performing UIKEE. Studies utilizing a bilateral training protocol were excluded as the focus of this review lied on unilateral training. Out of the 3,870 studies, which were reviewed, 20 studies fulfilled the selected inclusion criteria. These 20 studies were included in the BLMBMA to investigate the time-dependent effects of UIKEE. If compared to the baseline strength of the trained limb, these data reveal that UKIEE can increase the isometric strength by up to 46%. A meta-analysis based on the last time-point of each available study was employed to support further investigations into UIKEE-induced strength increase. A sensitivity analysis showed that intensity of training (%MVC), fraction of male subjects and the average age of the subject had no significant influence on the strength gain. Convergence of BLMBMA revealed that the peak strength increase is reached after ~4 weeks of UIKEE training.
RESUMEN
Although recent research emphasises the possible role of titin in skeletal muscle force enhancement, this property is commonly ignored in current computational models. This work presents the first biophysically based continuum-mechanical model of skeletal muscle that considers, in addition to actin-myosin interactions, force enhancement based on actin-titin interactions. During activation, titin attaches to actin filaments, which results in a significant reduction in titin's free molecular spring length and therefore results in increased titin forces during a subsequent stretch. The mechanical behaviour of titin is included on the microscopic half-sarcomere level of a multi-scale chemo-electro-mechanical muscle model, which is based on the classic sliding-filament and cross-bridge theories. In addition to titin stress contributions in the muscle fibre direction, the continuum-mechanical constitutive relation accounts for geometrically motivated, titin-induced stresses acting in the muscle's cross-fibre directions. Representative simulations of active stretches under maximal and submaximal activation levels predict realistic magnitudes of force enhancement in fibre direction. For example, stretching the model by 20 % from optimal length increased the isometric force at the target length by about 30 %. Predicted titin-induced stresses in the muscle's cross-fibre directions are rather insignificant. Including the presented development in future continuum-mechanical models of muscle function in dynamic situations will lead to more accurate model predictions during and after lengthening contractions.