[Biochemical parallels of cellular adaptive reactions at chronic low-intensity irradiation and action of phitoecdysteroid preparation serpisten].

Comparison of action of chronic γ-irradiation at a dose of 22.6 cGy and the serpisten substance containing phitoecdysteroids at small doses of 5 and 50 mg/kg on biochemical indicators in erythrocytes and tissues of white not purebred mice is given. It is established that in both cases there is an increase of minor fractions of cardiolipin and phosphatidic acid, lysophosphatidilcholin and a share of phospholipids as part of common lipids. Course administration of serpisten to rats at the total doses of 12 and 30 mg/kg leads to an increase in tissues of thermal shock proteins of family 70 (Hsp70 and Hsc70). Similarity of action of ecdysteroid preparations and the influence of stress factors of physical nature of low intensity (gamma radiation at a small dose) have been detected in mice, which manifest themselves in some chain links of lipid peroxidation processes as well as an increase in biosynthesis of thermal shock proteins of family 70 (Hsp70 and Hsc70) in rats at administration of serpisten.

[Evaluating adaptogenic properties of Rhodiola rosea extract in human mononuclear cell culture and rat tissues].

The administration of various doses of Rhodiola rosea roots and rhizome spissum with known concentrations of salidroside, n-tyrosol, and rosavine leads to an increase in stress proteins 70 content in human mononuclear cell culture and in tissues of Wistar rats. A one-week peroral administration of Rh. Rosea preparation increases the content of constitutive Hsc70 in liver and the amount of hepatocytes with low succinate dehydrogenase activity. A two-week administration of Rh. Rosea extract leads to an increase in the levels of inducible Hsp70 and constitutive Hsc70 proteins in liver, hippocampus and left heart ventricle. These results are indicative of an increase in nonspecific resistance and the activation of adaptogenic processes.

[HSC70 protein of human blood mononuclears as a sign of adaptation under normobaric hypoxia training].

We investigated two isoforms of heat shock protein 70 kDa: HSP70 and HSC70, in the human blood mononuclears under normobaric hypoxia training. It was shown that hypoxia regimen does not lead to manifestation of stress but exerts activation of the organism. The obtained organism adaptation is achieved with a little cost that is confirmed by absence of HSP70 content increase. HSC70 content in the blood mononuclears was increased in most case up to 1.5-2.0 fold. HSC70 displays itself as a sign of adaptation. We connect the increase of HSC70 with mitochondria biogenesis which is given a leading importance under adaptation of aerobic organism cells to hypoxia.

[Adaptation to hyperthermia and changes in peripheral blood leukocytes in humans]. / Adaptatsiia cheloveka k gipertermii i izmeneniia v leikotsitakh perifericheskoi krovi.

Heating humans up to rectal temperature 39.0-39.5 degrees C induces a heat stress and a physiological adaptation. Blood cells were found to produce nitric oxide under these conditions, neutrophiles playing a major role in the process. In our opinion, the HSC 70 takes part in the process of the long cell adaptation augmenting the cells' functional activity. Hyperthermia leads to a massive temporal HSP 72 expression in the blood mononuclears. This expression seems to be due to alterations in the cellular proteins and to oxidative stress.

[Effect of a short-term moderate whole body hyperthermia of healthy humans on their peripheral lymphocytes]. / Deistvie neprodolzhitel'nogo umerennogo nagrevaniia organizma zdorovogo cheloveka na perifericheskie limfotsity.

A short (75-110 min) mild (39.5 degrees C) whole body hyperthermia of humans does not alter the absolute amount of peripheral lymphocytes immediately after heating, but significantly reduces their relative amount due to neutrophil increase. Body heating does not induce lymphocyte degeneration: their potential viability does not reduce. The total level of lymphocyte oxidative metabolism remains unaltered, while SDG and LDG activities are seen reduced and increased, respectively. Lymphocytes adapt to body heating by inducing thermotolerant state and activating anaerobic metabolism.

[Aspects of healthy humans' response to hyperthermia]. / Osobennosti reaktsii organizma zdorovogo chekoveka na hipertermicheskoe vozdeistvie.

Single-shot hyperthermia of healthy young men in climate chamber up to rectal temperature of 39.5 degrees C over 75-110 minutes revealed variable heat resistance. Individuals with prevalent sympathetic vegetative regulation were more resistant to overheating, if compared to those with prevalent parasympathetic one. Endocrine group peculiarities were observed, the examinees with prevalent parasympathetic vegetative regulation demonstrated more drastic changes of some biochemical parameters.

[Rubomycin-induced cardiomyopathy in rats and the potential for therapy with a peptide preparation from the heart]. / Rubomitsinovaia kardiomiopatiia u krys i vozmozhnost' terapii peptidnym preparatom iz serdtsa.

The study has shown the efficiency of heart peptide preparation (HP) in the management of rubomycin cardiomyopathy. HP does not prevent rat mortality if rubomycin doses are very large, but it can improve a state of survived animals. HP affects restoration processes in myocardium improving the myocardial contractibility.

[The interrelationship of doses and the pharmacological effects of cardiac peptides]. / Vzaimosviaz' doz i farmakologicheskikh éffektov peptidov serdtsa.

Low-molecular weight peptides prepared according to an original procedure from a cattle heart possess a protecting effect on the myocardium during ischemia. Peptides in doses of 10(-7) g/ml and less improve the indexes of contractility function of the isolated heart after total ischemia and correct respiration warranting cardiomyocytes survival during hypoxia. However, in different models high doses of heart peptides (5 x 10(-6) g/ml and more) are shown to inhibit contractility, to stop respiration, the action of the peptides increasing with myocardial ischemia degree.

[Effect of a drug isolated from the heart on the bioenergetics of cardiomyocytes in hypoxia and ischemia]. / Vliianie preparata, vydelennogo iz serdtsa, na bioénergetiku kardiomiotsitov v usloviiakh gipoksii i ishemii.

It has been established that cordialin inhibits selectively succinic acid oxidation and intensifies the exudation of a NADH2 substrate which is energetically more advantageous, and in this way has a favorable effect on energy metabolism, evidence of which is increase of the ATP content in the myocardium. Cordialin correction of the biologic energy of cardiomyocytes in hypoxia and ischemia prevents the accumulation of lipid drops in the cells of the peri-infarction zone, which reflects the high intensity of the processes of fatty acids beta-oxidation. As a result of this glycolysis activation is unnecessary (which is confirmed by high glycogen content, both of a qualitative and quantitative character, in the myocardium and low concentrations of glucose and lactate in the blood) and, consequently, the sequelae of acidosis are prevented.

[Correction of tetramethylthiuram disulfide-induced damage to the myocardium by cardiac peptides]. / Korrektsiia peptidami serdtsa porazheniia miokarda tetrametiltiuramdisul'fidom.

Standardized complex of low-molecular peptides from the heart, cordialin, stimulates functional activity of cardiomyocytes and mobilizes their protective mechanisms against the effect of the industrial and agricultural poison tetramethylthiuram disulfide (TMTD). The cardioprotective effect of cordialin is most marked in the restoration period when it was administered after TMTD damage to the cardiomyocytes.

[Effects of a polypeptide drug on the state of energy metabolism of myocardial cells in hypoxic and ischemic conditions]. / Vliianie polipeptidnogo preparata na sostoianie énergeticheskogo metabolizma kletok miokarda, nakhodiashchikhsia v usloviiakh gipoksii i ishemii.

Cordialin, the agent extracted from the heart, is known to inhibit hyperoxidation of succinic acid, increasing NADH oxidation speed in suspension of cardiomyocytes in hypoxia. Cordialin presence in oxygenated cells' suspension oxidating succinate, doesn't change oxygen consumption speed. The results received may be a theoretical basis for cordialin utilization in therapy of myocardial diseases, associated with hypoxia and ischemia. Cordialin utilization may be recommended for the treatment of acute myocardial infarction and for prolongation of time-period for thrombolytic therapy, treatment of IMD, angina and other pathological states, in which oxygen transport disturbance to myocardium cells occurs.

[Effects of a cardiac peptide preparation on the myocardium in ischemia]. / Vliianie peptidnogo preparata iz serdtsa na miokard v usloviiakh ishemii.

Based on experimental studies the possibility of cordialin use in acute ischemia is being substantiated. On the first day of the animals' mortality and increased life duration of cells in ischemia zone, delaying the injury region expansion. But later in rats, that were given cordialin, slowing down of injury zone recovery and scar tissue formation was demonstrated. Cordialin use in early stages of myocardial infarction is suggested. In experiments on isolated heart cordialin is reported to decrease the intensity of processes of lipids' peroxide oxidation in intact and ischemic myocardium. But in reperfusion cordialin activates LPO, that is associated with heart contracting activity inhibition. The results of the study may serve as an experimental basis for cordialin use on the first day of MI development. Its further use needs the correction of its ability to slow down the processes of necrotic tissue recovery.