Exploring the influence of sediment motion on microplastic deposition in streambeds.

Microplastics (MP) of all sizes and densities have been found deposited in streambeds. Several delivery processes were proposed to explain these observations. However, none of the previous studies explored these processes systematically, especially in cases of streambeds made of fine sediments that are regularly in motion. In this study, we quantified the effect of streambed motion on the deposition and accumulation of MP in streambed sediments using particle tracking simulations in a numerical flow and transport model. The model was run for streamwater velocities of 0.1-0.5 m s-1 and median grain sizes of 0.15-0.6 mm. Streambed morphodynamics were estimated from these input parameters using empirical relationships. MP propensity to become trapped in porous media was simulated using a filtration coefficient. For each grain size and streamwater velocity, a wide variety of filtration coefficients was used in simulations in order to predict the fate of particles in the sediment. We found that exchange due to sediment turnover leads to burial and long-term deposition of MP that originally were not expected to enter the bed due to size exclusion. The results also show that in streambeds with fine sediments, localized deposits of MP are expected to occur as a horizontal layer below the moving fraction of the bed (upper layer). However, increasing celerity reduces the depth of MP deposition in the streambed. We conclude that models that do not include the effect of bed motion on MP deposition are likely miscalculating the deposition, retention, resuspensions and long-term accumulation of MP in streambed sediments.

The riverine bioreactor: An integrative perspective on biological decomposition of organic matter across riverine habitats.

Riverine ecosystems can be conceptualized as 'bioreactors' (the riverine bioreactor) which retain and decompose a wide range of organic substrates. The metabolic performance of the riverine bioreactor is linked to their community structure, the efficiency of energy transfer along food chains, and complex interactions among biotic and abiotic environmental factors. However, our understanding of the mechanistic functioning and capacity of the riverine bioreactor remains limited. We review the state of knowledge and outline major gaps in the understanding of biotic drivers of organic matter decomposition processes that occur in riverine ecosystems, across habitats, temporal dimensions, and latitudes influenced by climate change. We propose a novel, integrative analytical perspective to assess and predict decomposition processes in riverine ecosystems. We then use this model to analyse data to demonstrate that the size-spectra of a community can be used to predict decomposition rates by analysing an illustrative dataset. This modelling methodology allows comparison of the riverine bioreactor's performance across habitats and at a global scale. Our integrative analytical approach can be applied to advance understanding of the functioning and efficiency of the riverine bioreactor as hotspots of metabolic activity. Application of insights gained from such analyses could inform the development of strategies that promote the functioning of the riverine bioreactor across global ecosystems.

High-Resolution Integrated Transport Model for Studying Surface Water-Groundwater Interaction.

Transport processes that lead to exchange of mass between surface water and groundwater play a significant role for the ecological functioning of aquatic systems, for hydrological processes and for biogeochemical transformations. In this study, we present a novel integral modeling approach for flow and transport at the sediment-water interface. The model allows us to simultaneously simulate turbulent surface and subsurface flow and transport with the same conceptual approach. For this purpose, a conservative transport equation was implemented to an existing approach that uses an extended version of the Navier-Stokes equations. Based on previous flume studies which investigated the spreading of a dye tracer under neutral, losing and gaining flow conditions the new solver is validated. Tracer distributions of the experiments are in close agreement with the simulations. The simulated flow paths are significantly affected by in- and outflowing groundwater flow. The highest velocities within the sediment are found for losing condition, which leads to shorter residence times compared to neutral and gaining conditions. The largest extent of the hyporheic exchange flow is observed under neutral condition. The new solver can be used for further examinations of cases that are not suitable for the conventional coupled models, for example, if Reynolds numbers are larger than 10. Moreover, results gained with the integral solver provide high-resolution information on pressure and velocity distributions at the rippled streambed, which can be used to improve flow predictions. This includes the extent of hyporheic exchange under varying ambient groundwater flow conditions.

The effect of unsteady streamflow and stream-groundwater interactions on oxygen consumption in a sandy streambed.

Streamflow dynamics are often ignored when studying biogeochemical processes in the hyporheic zone. We explored the interactive effects of unsteady streamflow and groundwater fluxes on the delivery and consumption of oxygen within the hyporheic zone using a recirculating flume packed with natural sandy sediments. The flume was equipped with a programmable streamflow control and drainage system that was used to impose losing and gaining fluxes. Tracer tests were used to measure hyporheic exchange flux and a planar optode was used to measure subsurface oxygen concentration patterns. It was found that the volume of the oxic zone decreased when the losing flux declined, and was drastically decreased when gaining conditions were applied. It was also found that unsteady streamflow led to a slight increase in the average volume of the oxic zone, compared to the average volume of the oxic zone under steady streamflow. However, the average oxygen consumption rates were significantly higher under unsteady streamflow compared to steady streamflow under all groundwater conditions with the exception of the highest losing flux. The present study provides the first insight into the interactions between streamflow unsteadiness and losing/gaining fluxes and improve understanding of their impact on microbial metabolism in the hyporheic zone.

Author Correction: Environmental filtering and community delineation in the streambed ecotone.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

The effect of tertiary treated wastewater on fish growth and health: Laboratory-scale experiment with Poecilia reticulata (guppy).

Treated wastewater (TWW) constitutes a sustainable water resource and has been used for fish culture in some countries around the world, although there are no comprehensive data on the effect of TWW on fish growth and health in the context of aquaculture production. Our objectives were to examine how fish culture in TWW affected fish growth and fitness, as well as compliance with the international standards for safe consumption. Guppy (Poecilia reticulata) fingerlings were reared in 0%, 50% and 100% tertiary TWW (TTWW), from the age of five days, for a period of four months. In water analyses, 33 out of 67 tested organic micropollutants (OMPs) were detected in the TTWW samples at least once, at concentrations that are typically reported in domestic TTWW. Fish survival ranged between 77-80% and did not differ between treatment groups. Fish growth and mortality following challenge infection with Tetrahymena sp. (which ranged between 64-68%), were similar among treatment groups. Of tested immunological parameters, lysozyme and anti-protease was similar among treatments while complement activity was highest in the 50% TTWW-reared fish. No abnormalities were observed in the histopathological analysis. Levels of heavy metals, polychlorinated-biphenyls (PCBs) and organochlorines (OCs) in fish were below the detection limit and below the Food and Agriculture Organization of the United Nations (FAO) and the European Union EU maximal permitted levels in food fish. Results suggest that the yield of fish grown in TTWW is potentially similar to that in freshwater, and the produced fish comply with the standards of consumer safety. The results are in line with previous studies that examined the feasibility of TWW-fed aquaculture.

Simulating rewetting events in intermittent rivers and ephemeral streams: A global analysis of leached nutrients and organic matter.

Climate change and human pressures are changing the global distribution and the extent of intermittent rivers and ephemeral streams (IRES), which comprise half of the global river network area. IRES are characterized by periods of flow cessation, during which channel substrates accumulate and undergo physico-chemical changes (preconditioning), and periods of flow resumption, when these substrates are rewetted and release pulses of dissolved nutrients and organic matter (OM). However, there are no estimates of the amounts and quality of leached substances, nor is there information on the underlying environmental constraints operating at the global scale. We experimentally simulated, under standard laboratory conditions, rewetting of leaves, riverbed sediments, and epilithic biofilms collected during the dry phase across 205 IRES from five major climate zones. We determined the amounts and qualitative characteristics of the leached nutrients and OM, and estimated their areal fluxes from riverbeds. In addition, we evaluated the variance in leachate characteristics in relation to selected environmental variables and substrate characteristics. We found that sediments, due to their large quantities within riverbeds, contribute most to the overall flux of dissolved substances during rewetting events (56%-98%), and that flux rates distinctly differ among climate zones. Dissolved organic carbon, phenolics, and nitrate contributed most to the areal fluxes. The largest amounts of leached substances were found in the continental climate zone, coinciding with the lowest potential bioavailability of the leached OM. The opposite pattern was found in the arid zone. Environmental variables expected to be modified under climate change (i.e. potential evapotranspiration, aridity, dry period duration, land use) were correlated with the amount of leached substances, with the strongest relationship found for sediments. These results show that the role of IRES should be accounted for in global biogeochemical cycles, especially because prevalence of IRES will increase due to increasing severity of drying events.

Environmental filtering and community delineation in the streambed ecotone.

A current controversy in ecology is whether biological communities are discrete biological entities or simply study units created for convenience; a debate that becomes even more heated when delimiting communities along ecotones. Here, we report an interdisciplinary study designed to address the interplay between environmental drivers and community ecology in a typical ecotone ecosystem: the streambed. Environmental filtering at a micro-scale determined how diversity, productivity and composition of the whole streambed assemblage varied with depth and with the direction of vertical water exchange. Biomass and production decreased with increasing depth, and were lower under upwelling than downwelling conditions. However, the rate at which biomass and production decreased with increasing depth differed significantly for different taxonomic groups. Using quantitative biocenosis analysis, we also showed that benthic and hyporheic zone assemblages (assemblages in close juxtaposition) could be clearly distinguished as discrete communities with individual integrity. Vertical hydrodynamic conditions also influenced the demarcation between both communities; the benthic community reached greater depths in downwelling than in upwelling zones.

Endocrine disrupting compounds in streams in Israel and the Palestinian West Bank: Implications for transboundary basin management.

Endocrine disrupting compounds (EDCs) frequently enter surface waters via discharges from wastewater treatment plants (WWTPs), as well as from industrial and agricultural activities, creating environmental and health concerns. In this study, selected EDCs were measured in water and sediments along two transboundary streams flowing from the Palestinian Authority (PA) into Israel (the Zomar-Alexander and Hebron-Beer Sheva Streams). We assessed how the complicated conflict situation between Israel and the PA and the absence of a coordinated strategy and joint stream management commission influence effective EDC control. Both streams receive raw Palestinian wastewater in their headwaters, which flows through rural areas and is treated via sediment settling facilities after crossing the 1949 Armistice Agreement Line. Four sampling campaigns were conducted over two years, with concentrations of selected EDCs measured in both the water and the sediments. Results show asymmetrical pollution profiles due to socio-economic differences and contrasting treatment capacities. No in-stream attenuation was observed along the stream and in the sediments within the Palestinian region. After sediment settling in treatment facilities at the Israeli border, however, significant reductions in the EDC concentrations were measured both in the sediments and in the water. Differences in sedimentation technologies had a substantial effect on EDC removal at the treatment location, positively affecting the streams' ability to further remove EDCs downstream. The prevailing approach to addressing the Israeli-Palestinian transboundary wastewater contamination reveals a narrow perspective among water managers who on occasion only take local interests into consideration, with interventions focused solely on improving stream water quality in isolated segments. Application of the "proximity principle" through the establishment of WWTPs at contamination sources constitutes a preferable strategy for reducing contamination by EDCs and other pollutants to ensure minimization of public health risks due to the pollution of streams and underlying potable groundwater.

A simple model for estimating the concentrations of natural estrogens in raw wastewater.

This study provides a tool for predicting the concentrations of the natural estrogens (NEs) estrone, 17ß-estradiol and estriol in raw wastewater (WW). Data characterizing the biochemical oxygen demand (BOD), NE concentrations, and discharges of raw sewage to wastewater treatment plants (WWTPs) were collected from various publications and used in the model formulation. A strong correlation was found between the log transformed BOD and the log transformed estrone load (r2=0.84, n=61), the log transformed 17ß-estradiol load (r2=0.89, n=52) and the log transformed estriol load (r2=0.80, n=40). The models are reasonably accurate when compared to the measured concentrations and slightly better than previous modeling efforts. The relative amounts of data falling within ±50% error were 67% for estrone, 63% for 17ß-estradiol, and 55% for estriol. Because the model was developed from a wide array of WWTPs from five continents, it is universal and can be used for projecting concentrations of NEs from a wide range of mixed domestic and industrial sources, but may be less precise when sources contain high levels of NEs or BOD (e.g., WW from dairy farms and food processing plants). The model is expected to improve our ability to predict the fate of NEs in WWTPs and in the receiving environment, which currently relies on estimating the concentrations of NEs in raw wastewater. Its application is especially valuable since direct measurement of NEs in raw WW is expensive and practically impossible in many developing countries due to the lack of expertise and funds.

Occurrence and fate of endocrine disrupting compounds in wastewater treatment plants in Israel and the Palestinian West Bank.

Israel and its Palestinian neighbors constitute a unique venue for evaluating the treatment efficiency and potential environmental risks of endocrine disrupting compounds (EDCs) in wastewater treatment plants (WWTPs), because of their physical proximity yet contrasting societal dynamics. Israel primarily relies on advanced tertiary sewage treatment and recycles over 85% of its treated wastewater, while in the Palestinian Authority (PA), there is only secondary treatment levels at WWTPs and reuse is minimal (<1%). To evaluate the extent of EDC occurrence and treatment efficiency, we conducted four sampling campaigns over two consecutive years, and measured the concentrations of selected EDCs in raw wastewater (WW), treated WW and sludge in six WWTPs in Israel, as well as in two Palestinian plants. Low concentrations of bisphenol A, octylphenol and triclosan measured in the raw WW in the Palestinian WWTPs reflected the relatively modest industrial activity and consumption habits as compared to the westernized consumer patterns in Israel. On the other hand, hormone concentrations in raw WW were higher in the Palestinian WWTPs than those in the Israeli WWTPs, presumably because of a dilution effect associated with a higher water per capita consumption among Israelis. Despite these differences in raw WW concentrations, the removal efficiency in all advanced WWTPs was relatively high when compared to averages reported internationally.

Impact of treated wastewater reuse and floods on water quality and fish health within a water reservoir in an arid climate.

Treated wastewater (TWW) reuse for agricultural irrigation is a well-established approach to coping with water shortages in semi-arid and arid environments. Recently, additional uses of TWW have emerged, including streamflow augmentation and aquatic ecosystem restoration. The purpose of the current study was to evaluate the water quality and fish health, in an artificial reservoir located in an arid climate (the Yeruham Reservoir, Israel), which regularly receives TWW and sporadic winter floods. The temporal distribution of water levels, nutrients and organic micropollutants (OMPs) were measured during the years 2013-2014. OMPs were also measured in sediment and fish tissues. Finally, the status of fish health was evaluated by histopathology. Water levels and quality were mainly influenced by seasonal processes such as floods and evaporation, and not by the discharge of TWW. Out of 16 tested OMPs, estrone, carbamazepine, diclofenac and bezafibrate were found in the reservoir water, but mostly at concentrations below the predicted no-effect concentration (PNEC) for fish. Concentrations of PCBs and dioxins in fish muscle and liver were much lower than the EU maximal permitted concentrations, and similar to concentrations that were found in food fish in Israel and Europe. In the histopathological analysis, there were no evident tissue abnormalities, and low to moderate infection levels of fish parasites were recorded. The results from the Yeruham Reservoir demonstrated a unique model for the mixture effect between TWW reuse and natural floods to support a unique stable and thriving ecosystem in a water reservoir located in an arid region. This type of reservoir can be widely used for recreation, education, and the social and economic development of a rural environment, such as has occurred in the Yeruham region.

Why endocrine disrupting chemicals (EDCs) challenge traditional risk assessment and how to respond.

Endocrine disrupting compounds (EDCs) are a diverse group of "chemicals of emerging concern" which have attracted much interest from the research community since the 1990s. Today there is still no definitive risk assessment tool for EDCs. While some decision making organizations have attempted to design methodology guidelines to evaluate the potential risk from this broadly defined group of constituents, risk assessors still face many uncertainties and unknowns. Until a risk assessment paradigm is designed specifically for EDCs and is vetted by the field, traditional risk assessment tools may be used with caution to evaluate EDCs. In doing so, each issue of contention should be addressed with transparency in order to leverage available information and technology without sacrificing integrity or accuracy. The challenges that EDCs pose to traditional risk assessment are described in this article to assist in this process.

Impact of streambed morphology on the abundance and activity of ammonia-oxidizing bacteria.

Ammonia oxidizers catalyze the first step of nitrification. Combined microbial nitrification-denitrification activities are essential for the removal of excess nitrogen from water bodies. In sandy streambeds, bed form structures are created by water flow and lead to the creation of heterogeneous microenvironments. The objective of this study, therefore, was to investigate the effect of bed form morphology on the abundance and activity of ammonia-oxidizing bacteria (AOB) within a benthic biofilm. An 8-month-old benthic biofilm was established in a recirculating laboratory flume under controlled flow conditions and frequent amendment with ammonium. The sand bed was arranged into bed form structures. The highest concentrations of chlorophyll a (indicative of algae) were measured on the upstream side of the bed forms. The biofilm was dominated by Nitrosospira species, and amoA gene abundance was higher on the downstream sides of the bed forms with no significant difference in oxygen consumption between the upstream and downstream sections of the bed form. In contrast, potential ammonium oxidation rates were higher on the upstream sides of the bed forms. The results suggest that bed form morphology can affect the spatial distribution and activity of AOB, possibly through the creation of distinct microhabitats. These results contribute to our understanding of nitrogen transformations and removal from streams.

Transport of testosterone and estrogen from dairy-farm waste lagoons to groundwater.

Although concentrated animal feeding operations constantly generate physiologically active steroidal hormones, little is known of their environmental fate. Estrogen and testosterone concentrations in groundwater and their distribution in sediments below a dairy-farm wastewater lagoon were therefore determined and compared to a reference site located upgradient of the farm. Forward simulations of flow as well as estrogen and testosterone transport were conducted based on data from the sediment profile obtained during drilling of a monitoring well belowthe dairy-farm waste lagoon. Testosterone and estrogen were detected in sediments to depths of 45 and 32 m, respectively. Groundwater samples were directly impacted by the dairy farm, as evidenced by elevated concentrations of nitrate, chloride, testosterone, and estrogen as compared to the reference site. Modeling potential transport of hormones in the vadose zone via advection, dispersion, and sorption could not explain the depths at which estrogen and testosterone were found, suggesting that other transport mechanisms influence hormone transport under field conditions. These mechanisms may involve interactions between hormones and manure as well as preferential flow paths, leading to enhanced transport rates. These types of interactions should be further investigated to understand the processes regulating hormone transport in the subsurface environment and parametrized to forecast long-term fate and transport of steroidal hormones.

Influence of algal community structure on denitrification rates in periphyton cultivated on artificial substrata.

We conducted a field survey of periphyton cultivated on benthic mesh installations in freshwater aquatic systems, including two constructed wetlands and a pond, and also studied periphyton grown on a benthic mesh in laboratory mesocosms. The objectives of this study were to (1) determine if periphyton cultivated on benthic mesh denitrifies at higher rates than the underlying sediments and (2) determine if denitrification rates within periphyton vary with characteristics such as algal and bacterial community structure and biomass. We measured denitrification potential rates of field and laboratory periphyton by the acetylene inhibition method. We characterized algal community composition by algal identification and bacterial community composition by terminal restriction fragment length polymorphisms. Periphyton collected on benthic mesh from our field sites denitrified at significantly higher rates than the underlying sediments, regardless of sampling site or season. Results from both our field survey and laboratory studies indicated a significant, positive correlation between diatom presence and denitrification rate. In our laboratory studies, we found that periphyton with the highest diatom abundance showed the highest denitrification rates as well as a distinct bacterial community composition. These results suggest a synergistic relationship between diatoms and denitrifying bacteria that warrants further study.

Influence of flow conditions and system geometry on nitrate use by benthic biofilms: implications for nutrient mitigation.

The effects of substratum geometry and overlying velocity on nitrate use by periphyton were assessed. Periphyton was cultivated at an average current velocity of 0.5 cm s(-1) in laboratory mesocosms (120 cm long, 60 cm wide) on polyethylene nets of three different geometries, "1-lay er", "3-layer", and "bedform" structures, overlaying a thin bed of sand. Bulk nitrate use was then measured as the reduction of nitrate concentration in the overlying water under average velocities of 0.005, 0.05, and 0.5 cm s(-1). Periphyton structural characteristics were quantified as algal/bacterial biomass, algal species composition, and bacterial densities. Accrual of microbial biomass increased monotonically with increasing benthic net surface area, with upper sections of structures supporting the highest biomass. Maximum rates of nitrate removal were measured in the bedform geometry at intermediate velocity (173 mg NO3-N m(-2) d(-1)), and the lowest was measured with 1-layer geometry at the fastest velocity (11 mg NO3-N m(-2) d(-1)). Oxygen microprofiles within biofilms demonstrated that hydrodynamic conditions and benthic structure both play a key role in the regulation of microbial processing of nitrate delivered from the water column by promotion of denitrification in downstream sections of bedform substrata. Interactions between hydrodynamic conditions and substratum geometry are expected to regulate microbial activity in all surficial natural and engineered environments and must be parameterized to forecast long-term average biochemical transformation rates in rivers and other dynamic aquatic systems.

Evaluation of soil flushing potential for clean-up of desert soil contaminated by industrial wastewater.

The flushing potential of a desert loess soil contaminated by the flame retardant Tetrabromobisphenol A (TBBPA), chloride (Cl(-)) and bromide (Br(-)) was studied in undisturbed laboratory column experiments (20 cm diameter, 45 cm long) and a small field plot (2 x 2 m). While the soluble inorganic ions (Cl(-) and Br(-)) were efficiently flushed from the soil profile after less than three pore volumes (PV) of water, about 50% of the initial amount of TBBPA in the soil was also flushed, despite its hydrophobic nature. TBBPA leaching was made possible due to a significant increase in the pH of the soil solution from 7.5 to 9, which increased TBBPA aqueous solubility. The remaining TBBPA mass in the soil was not mobilized from its initial location in the topsoil due to the decrease in pH at this horizon. In situ soil flushing demonstrated that this method is a feasible treatment for reducing soil contamination at this site.

Two-dimensional distribution of microbial activity and flow patterns within naturally fractured chalk.

The two-dimensional distribution of flow patterns and their dynamic change due to microbial activity were investigated in naturally fractured chalk cores. Long-term biodegradation experiments were conducted in two cores ( approximately 20 cm diameter, 31 and 44 cm long), intersected by a natural fracture. 2,4,6-tribromophenol (TBP) was used as a model contaminant and as the sole carbon source for aerobic microbial activity. The transmissivity of the fractures was continuously reduced due to biomass accumulation in the fracture concurrent with TBP biodegradation. From multi-tracer experiments conducted prior to and following the microbial activity, it was found that biomass accumulation causes redistribution of the preferential flow channels. Zones of slow flow near the fracture inlet were clogged, thus further diverting the flow through zones of fast flow, which were also partially clogged. Quantitative evaluation of biodegradation and bacterial counts supported the results of the multi-tracer tests, indicating that most of the bacterial activity occurs close to the inlet. The changing flow patterns, which control the nutrient supply, resulted in variations in the concentrations of the chemical constituents (TBP, bromide and oxygen), used as indicators of biodegradation.