RESUMEN
Multilocus analysis was for the first time used to study the phylogeny of the Crocidura suaveolens s. l. species complex. Sequencing data for 16 nuclear genes indicated that several distinct forms exist within the species complex. The structure of the complex did generally not contradict its mitochondrial phylogeny. Siberian shrew showed certain specificity of the nuclear genome, but the degree of its genetic differentiation did not correspond to the species level. Relationships of Crocidura aff. suaveolens from South Gansu and Sichuan with other forms of the species complex were clarified. Shrews from Buryatia and Khentei also belong to this form, but their mtDNA apparently introgressed from C. shantungensis in the past. Hybridization of C. suaveolens s. str. with C. aff. suaveolens and C. güeldenstaedtii occurred recently. Due to multiple introgression events in the history of C. suaveolens s. l., a far larger set of loci is necessary for the analysis of the phylogenetic relationships between its forms.
Asunto(s)
ADN Mitocondrial , Musarañas , Animales , Filogenia , Musarañas/genética , ADN Mitocondrial/genéticaRESUMEN
Phylogeography of the steppe bat, Myotis davidii in the eastern part of its broad range, was explored for the first time using mitochondrial genetic markers. The presence of two main intraspecific clades, Eastern and Western, was confirmed. Definite inner structure inside the Eastern group was shown. We discovered genetic diversity hotspot in northwestern Mongolia and neighboring regions, where highly divergent haplotypes are found. Presumably, this can be explained by Pleistocene refugial structure shaped by the ridges of the Mongolian Altai. The haplogroups from the southeast of Mongolia and Transbaikalia found to be related, while populations of the Kerulen valley located between these regions carry more distant haplotypes.
Asunto(s)
Quirópteros , Animales , Quirópteros/genética , Haplotipos/genética , Mongolia , Filogenia , FilogeografíaRESUMEN
There are evidences that the genus Pipistrellus (true pipistrelles) in its current understanding may be paraphyletic. We have studied for the first time the phylogenetic relationships between the majority of supraspecific taxa of the subtribe Pipistrellina based on the analysis of the sequences of three nuclear genes (RAG2, APOB, and DMP1). Our data support the monophyly of the subtribe itself, but definitely show that the genus Pipistrellus in the modern sense is paraphyletic with respect to noctules, Nyctalus, and thick thumb pipistrelles, Glischropus (which both are morphologically distinct taxa). Moreover, noctules are extremely close to the "western" clade of Pipistrellus. The latter genus should probably be divided into two or more separate genera, or Nyctalus and Glischropus should be treated as its subgenera.
Asunto(s)
Núcleo Celular , Quirópteros , Animales , Filogenia , Análisis de Secuencia de ADN , Núcleo Celular/genética , Quirópteros/genética , Marcadores GenéticosRESUMEN
The genus Hipposideros is one of the largest bat genera. The latest research on its Asian representatives has been done only with mitochondrial or sole nuclear markers. In the present study we compare the results of phylogenetic reconstructions, based on mitochondrial, on single nuclear genes and on multiple nuclear markers analyzed using the MRP algorithm (Matrix representation with parsimony) for representative species from all the main species groups. MRP analysis provides the most supported and well-interpretable phylogeny. Our data indicate monophyly of the large Asian leaf-nosed bats which suggests they are an independent taxon of the subgenus rank, as well as monophyly of several species complexes which are paraphyletic according to mtDNA analysis.
