RESUMEN
CONTEXT: Thyroglobulin (TG) gene mutations cause congenital hypothyroidism (CH) with goiter. A founder effect has been proposed for some frequent mutations. Mutated proteins have a defect in intracellular transport causing intracellular retention with ultrastructural changes that resemble an endoplasmic reticulum storage disease. OBJECTIVE: To reveal new aspects of thyroglobulin pathophysiology through clinical, cellular, molecular, and genetic studies in a family presenting with CH due to TG mutations from Galicia, an iodine-deficient area of Spain. DESIGN: The included clinical evaluation of family members, DNA sequencing for TG gene mutation and haplotyping analysis, ultrastructural analysis of thyroid tissue specimens from affected subjects, analysis of effects of mutations found on TG gene transcription, and in vitro studies of cellular production and secretion of mutated proteins. SETTING: Locations included primary care and university hospitals. RESULTS: Family members with CH, mental retardation, and goiter were compound heterozygous for c.886C-->T (p.R277X) and g.IVS35+1delG. For c.886C-->T, a founder effect cannot be excluded, and its transcription was hardly detectable. g.IVS35+1delG caused an in-frame deletion in exon 35 and produced a protein that, although synthesized, could not be secreted. Ultrastructural analyses showed morphological changes consistent with an endoplasmic reticulum storage disease. CONCLUSION: The shorter thyroglobulin resulting from the novel g.IVS35+1delG was retained within the endoplasmic reticulum of thyrocytes, and together with p.R227X caused severe hypothyroidism with goiter. p.R277X, the most commonly described TG mutation, is caused by a TG exon-7 highly mutation-prone region, and the possibility that some cases were introduced to South America from Galicia cannot be excluded.
Asunto(s)
Hipotiroidismo Congénito/genética , Bocio/genética , Tiroglobulina/genética , Adulto , Western Blotting , Células Cultivadas , Pruebas Genéticas , Haplotipos , Humanos , Inmunoprecipitación , Masculino , Microscopía Electrónica , Mutación/genética , Linaje , EspañaRESUMEN
The MAL proteolipid is an integral membrane protein identified as a component of the raft machinery for apical sorting of membrane proteins in Madin-Darby canine kidney (MDCK) cells. Previous studies have implicated lipid rafts in the transport of exogenous thyroglobulin (Tg), the predominant secretory protein of thyroid epithelial cells, to the apical surface in MDCK cells. We have examined the secretion of recombinant Tg and gp80/clusterin, a major endogenous secretory protein not detected in Triton X-100 insoluble rafts, for the investigation of the involvement of MAL in the constitutive apical secretory pathway of MDCK cells. We show that MAL depletion impairs apical secretion of Tg and causes its accumulation in the Golgi. Cholesterol sequestration, which blocks apical secretion of Tg, did not alter the levels of MAL in rafts but created a block proximal to Tg entrance into rafts. Apical secretion of gp80/clusterin was also inhibited by elimination of endogenous MAL. Our results suggest a role for MAL in the transport of both endogenously and exogenously expressed apical secretory proteins in MDCK cells.
Asunto(s)
Proteínas Portadoras/metabolismo , Células Epiteliales/metabolismo , Proteínas de Transporte de Membrana , Proteínas de la Mielina , Transporte de Proteínas/fisiología , Proteolípidos/metabolismo , Tiroglobulina/metabolismo , beta-Ciclodextrinas , Animales , Línea Celular , Polaridad Celular , Colesterol/metabolismo , Clusterina , Ciclodextrinas/farmacología , Perros , Células Epiteliales/efectos de los fármacos , Glicoproteínas/metabolismo , Aparato de Golgi/metabolismo , Riñón/citología , Microdominios de Membrana/metabolismo , Proteínas de la Membrana/metabolismo , Modelos Biológicos , Chaperonas Moleculares/metabolismo , Proteínas Proteolipídicas Asociadas a Mielina y Linfocito , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Tiroglobulina/genéticaRESUMEN
An insulin-containing fusion protein (ICFP, encoding the yeast prepro-alpha factor leader peptide fused via a lysine-arginine cleavage site to a single chain insulin) has been expressed in Saccharomyces cerevisiae where it is inefficiently secreted. Single gene disruptions have been identified that cause enhanced immunoreactive insulin secretion (eis). Five out of six eis mutants prove to be vacuolar protein sorting (vps)8, vps35, vps13, vps4, and vps36, which affect Golgi<-->endosome trafficking. Indeed, in wild-type yeast insulin is ultimately delivered to the vacuole, whereas vps mutants secrete primarily unprocessed ICFP. Disruption of KEX2, which blocks intracellular processing to insulin, quantitatively reroutes ICFP to the cell surface, whereas loss of the Vps10p sorting receptor is without effect. Secretion of unprocessed ICFP is not based on a dominant secretion signal in the alpha-leader peptide. Although insulin sorting mediated by Kex2p is saturable, Kex2p functions not as a sorting receptor but as a protease: replacement of Kex2p by truncated secretory Kex2p (which travels from Golgi to cell surface) still causes endoproteolytic processing and intracellular insulin retention. Endoproteolysis promotes a change in insulin's biophysical properties. B5His residues normally participate in multimeric insulin packing; a point mutation at this position permits ICFP processing but causes the majority of processed insulin to be secreted. The data argue that multimeric assembly consequent to endoproteolytic maturation regulates insulin sorting in the secretory pathway.
Asunto(s)
Proteínas Fúngicas/metabolismo , Aparato de Golgi/metabolismo , Insulina/metabolismo , Proproteína Convertasas , Precursores de Proteínas/metabolismo , Procesamiento Proteico-Postraduccional , Proteínas de Saccharomyces cerevisiae , Subtilisinas/metabolismo , Proteínas de Transporte Vesicular , Secuencia de Aminoácidos , Proteínas Fúngicas/genética , Secreción de Insulina , Líquido Intracelular/metabolismo , Datos de Secuencia Molecular , Mutagénesis , Precursores de Proteínas/genética , Receptores de Superficie Celular/metabolismo , Saccharomyces cerevisiae/metabolismo , Transducción de Señal/fisiología , Subtilisinas/genéticaRESUMEN
Caveolin-1 serves as the main coat protein of caveolae membranes, as an intracellular cholesterol shuttle, and as a regulator of diverse signaling molecules. Of the 12 residues conserved across all caveolin isoforms from all species examined to date, only Ser(80) and Ser(168) could serve as phosphorylation sites. We show here that mimicking chronic phosphorylation of Ser(80) by mutation to Glu (i.e. Cav-1(S80E)), blocks phosphate incorporation. However, Cav-1(S168E) is phosphorylated to the same extent as wild-type caveolin-1. Cav-1(S80E) targets to the endoplasmic reticulum membrane, remains oligomeric, and maintains normal membrane topology. In contrast, Cav-1(S80A), which cannot be phosphorylated, targets to caveolae membranes. Some exocrine cells secrete caveolin-1 in a regulated manner. Cav-1(S80A) is not secreted by AR42J pancreatic adenocarcinoma cells even in the presence of dexamethasone, an agent that induces the secretory phenotype. Conversely, Cav-1(S80E) is secreted to a greater extent than wild-type caveolin-1 following dexamethasone treatment. We conclude that caveolin-1 phosphorylation on invariant serine residue 80 is required for endoplasmic reticulum retention and entry into the regulated secretory pathway.
Asunto(s)
Caveolinas/metabolismo , Retículo Endoplásmico/metabolismo , Transporte de Proteínas , Serina/metabolismo , Secuencia de Aminoácidos , Animales , Caveolina 1 , Caveolinas/química , Caveolinas/genética , Línea Celular , Perros , Humanos , Datos de Secuencia Molecular , Mutagénesis , Fosforilación , Pruebas de Precipitina , Unión Proteica , Homología de Secuencia de AminoácidoRESUMEN
Thyroid hormone synthesis by thyrocytes depends upon apical secretion of thyroglobulin (Tg), the glycoprotein prohormone. In stably transfected MDCK cells, recombinant Tg is also secreted apically. All secreted Tg has undergone Golgi carbohydrate modification, whereas most intracellular Tg (which is slow to exit the endoplasmic reticulum) is sensitive to digestion with endoglycosidase H. However, in MDCK cells and PC Cl3 thyrocytes, a subpopulation of newly synthesized recombinant and endogenous Tg, respectively, is recovered in a Triton X-100 insoluble, glycosphingolipid/cholesterol-enriched (GEM/raft) fraction, and this small subpopulation is overwhelmingly endoglycosidase H resistant. Upon apical secretion, Tg solubility is restored. Apical secretion of Tg is inhibited by cellular cholesterol depletion. In FRT cells, recombinant Tg becomes Triton X-100 insoluble within 60 min after synthesis and a portion is actually endoglycosidase H-sensitive, suggesting early Tg entry into GEMs/rafts. Interestingly in FRT cells, Tg remains associated with the apical plasma membrane upon exocytosis, and all surface Tg is GEM/raft-associated. Thus, Tg is the first secretory protein demonstrated to enter Triton X-100 insoluble membranes en route to the apical surface of epithelial cells. The data imply that Tg utilizes a cargo-selective mechanism for apical sorting.
Asunto(s)
Tiroglobulina/metabolismo , Animales , Transporte Biológico , Biotinilación , Membrana Celular/metabolismo , Células Cultivadas , Colesterol/metabolismo , Perros , Células Epiteliales/metabolismo , Glicoesfingolípidos/metabolismo , Riñón/metabolismo , Octoxinol/farmacologíaRESUMEN
Warfarin, an antagonist of vitamin K, causes diminution of vitamin K-dependent coagulation factors in the circulation. Although all vitamin K-dependent factors have Gla domains, the warfarin-induced decrease in their plasma concentration differs among factors. In warfarin-treated HepG2 cells, we found modest and severe intracellular degradation of prothrombin and protein C, respectively. To investigate the structural features of these proteins that contribute to their warfarin sensitivity, chimeric prothrombin containing the prepropeptide and Gla domain of protein C was expressed in baby hamster kidney (BHK) cells. This chimera showed similar secretion kinetics and warfarin sensitivity to those of wild-type prothrombin, demonstrating that the Gla domain cannot solely explain the warfarin sensitivity of protein C. In contrast, two chimeric protein Cs containing either the Gla domain alone or the prepropeptide and Gla domain of prothrombin showed impaired secretion. Even though gamma-carboxylation proceeded normally, both chimeras were degraded intracellularly by the proteasome. From these results, we conclude that not only the folding of the Gla domain, but the entire structure and conformation of protein C and prothrombin, contribute to their quality control and susceptibility to warfarin-induced ER (endoplasmic reticulum)-associated degradation.
Asunto(s)
Retículo Endoplásmico/metabolismo , Proteína C/química , Protrombina/química , Proteínas Recombinantes de Fusión/química , Adenosina Trifosfatasas/metabolismo , Adenosina Trifosfatasas/farmacología , Secuencia de Aminoácidos , Animales , Cricetinae , Cisteína Endopeptidasas/metabolismo , Cisteína Endopeptidasas/farmacología , Electroforesis en Gel de Poliacrilamida , Humanos , Datos de Secuencia Molecular , Complejos Multienzimáticos/metabolismo , Complejos Multienzimáticos/farmacología , Complejo de la Endopetidasa Proteasomal , Proteína C/efectos de los fármacos , Proteína C/metabolismo , Pliegue de Proteína , Estructura Terciaria de Proteína , Protrombina/efectos de los fármacos , Protrombina/metabolismo , Proteínas Recombinantes de Fusión/efectos de los fármacos , Proteínas Recombinantes de Fusión/metabolismo , Alineación de Secuencia , Transfección , Células Tumorales Cultivadas , Vitamina K/farmacología , Warfarina/farmacologíaRESUMEN
To examine the role of early carbohydrate recognition/trimming reactions in targeting endoplasmic reticulum (ER)-retained, misfolded glycoproteins for ER-associated degradation (ERAD), we have stably expressed the cog thyroglobulin (Tg) mutant cDNA in Chinese hamster ovary cells. We found that inhibitors of ER mannosidase I (but not other glycosidases) acutely suppressed Cog Tg degradation and also perturbed the ERAD process for Tg reduced with dithiothreitol as well as for gamma-carboxylation-deficient protein C expressed in warfarin-treated baby hamster kidney cells. Kifunensine inhibition of ER mannosidase I also suppressed ERAD in castanospermine-treated cells; thus, suppression of ERAD does not require lectin-like binding of ER chaperones calnexin and calreticulin to monoglucosylated oligosaccharides. Notably, the undegraded protein fraction remained completely microsome-associated. In pulse-chase studies, kifunensine-sensitive degradation was still inhibitable even 1 h after Tg synthesis. Intriguingly, chronic treatment with kifunensine caused a 3-fold accumulation of Cog Tg in Chinese hamster ovary cells and did not lead to significant induction of the ER unfolded protein response. We hypothesize that, in a manner not requiring lectin-like activity of calnexin/calreticulin, the recognition or processing of a specific branched N-linked mannose structure enhances the efficiency of glycoprotein retrotranslocation from the ER lumen.
Asunto(s)
Alcaloides/farmacología , Retículo Endoplásmico/metabolismo , Inhibidores Enzimáticos/farmacología , Glicoproteínas/metabolismo , Manosidasas/fisiología , Tiroglobulina/metabolismo , Animales , Células CHO , Cricetinae , Citosol/metabolismo , Glicoproteínas/química , Manosidasas/antagonistas & inhibidores , Proteína C/metabolismo , Pliegue de ProteínaRESUMEN
Recently, it has been suggested that only approximately 2% of human thyroid peroxidase (hTPO(933)) reaches the surface of stably transfected (Chinese hamster ovary) cells, most being degraded intracellularly, and this might be representative of thyroid peroxidase (TPO) behavior in thyrocytes (Fayadat, L., Siffroi-Fernandez, S., Lanet, J., and Franc, J.-L. (2000) J. Biol. Chem. 275, 15948-15954). In agreement, in stably transfected Madin-Darby canine kidney clones, nonpermeabilized cells exhibit wild-type hTPO(933) immunofluorescence (apically) on <10% of that found in permeabilized cells, where an endoplasmic reticulum pattern is observed. Further, a C-terminally truncated, membrane-anchorless hTPO(848) is also retained in the endoplasmic reticulum of stably transfected Madin-Darby canine kidney cells. However, by contrast, in Chinese hamster ovary cells after transient transfection, hTPO(933) immunofluorescence is detected equally well in nonpermeabilized and permeabilized cells, indicating that a large portion of hTPO(933) is present at the cell surface; furthermore, hTPO(848) is efficiently secreted. Further, using an antiserum not cross-reacting with rat TPO, we find by immunofluorescence that in stable clones of PC Cl3 (rat) thyrocytes, considerably more ( approximately 50%) of the cells exhibit hTPO(933) at the cell surface. However, cell surface biotinylation and endoglycosidase H digestion assays appear to under-represent the extent of hTPO(933) transport, presumably because protein folding limits both Golgi carbohydrate modification and accessibility of lysines in the extracellular domain. We conclude that cell type-specific factors may facilitate stable expression of TPO at the cell surface of thyrocytes.
Asunto(s)
Membrana Celular/enzimología , Yoduro Peroxidasa/metabolismo , Proteínas de la Membrana/metabolismo , Glándula Tiroides/citología , Glándula Tiroides/enzimología , Animales , Biotinilación , Células CHO , Línea Celular , Membrana Celular/metabolismo , Permeabilidad de la Membrana Celular , Cricetinae , Perros , Estabilidad de Enzimas , Técnica del Anticuerpo Fluorescente , Hexosaminidasas/metabolismo , Humanos , Yoduro Peroxidasa/genética , Proteínas de la Membrana/genética , Especificidad de Órganos , Transporte de Proteínas , Ratas , Proteínas Recombinantes/metabolismo , Eliminación de Secuencia , Especificidad de la Especie , Glándula Tiroides/metabolismo , TransfecciónRESUMEN
Recently, two different prohormone-processing enzymes, prohormone convertase 1 (PC1) and carboxypeptidase E, have been implicated in enhancing the storage of peptide hormones in endocrine secretory granules. It is important to know the extent to which such molecules may act as "sorting receptors" to allow the selective trafficking of cargo proteins from the trans-Golgi network into forming granules, versus acting as enzymes that may indirectly facilitate intraluminal storage of processed hormones within maturing granules. GH4C1 cells primarily store prolactin in granules; they lack PC1 and are defective for intragranular storage of transfected proinsulin. However, proinsulin readily enters the immature granules of these cells. Interestingly, GH4C1 clones that stably express modest levels of PC1 store more proinsulin-derived protein in granules. Even in the presence of PC1, a sizable portion of the proinsulin that enters granules goes unprocessed, and this portion largely escapes granule storage. Indeed, all of the increased granule storage can be accounted for by the modest portion converted to insulin. These results are not unique to GH4C1 cells; similar results are obtained upon PC1 expression in PC12 cells as well as in AtT20 cells (in which PC1 is expressed endogenously at higher levels). An in vitro assay of protein solubility indicates a difference in the biophysical behavior of proinsulin and insulin in the PC1 transfectants. We conclude that processing to insulin, facilitated by the catalytic activities of granule proteolytic enzymes, assists in the targeting (storage) of the hormone.
Asunto(s)
Ácido Aspártico Endopeptidasas/biosíntesis , Proinsulina/metabolismo , Procesamiento Proteico-Postraduccional , Transducción de Señal , Animales , Ácido Aspártico Endopeptidasas/genética , Línea Celular , Gránulos Citoplasmáticos/metabolismo , Expresión Génica , Humanos , Modelos Biológicos , Células PC12 , Proinsulina/genética , Prolactina/metabolismo , Proproteína Convertasas , Ratas , Factores de Tiempo , TransfecciónRESUMEN
Constitutive-like secretion involves vesicular trafficking corresponding kinetically and biochemically with a post-trans-Golgi network (TGN) origin. In pancreatic beta-cells, the budding of AP-1/clathrin-coated vesicles, a portion of which is derived from immature secretory granules, has been hypothesized to initiate constitutive-like trafficking. However, approximately 30 min after release of a 20 degrees C intracellular transport block in pancreatic beta-cells (to synchronize protein egress from the TGN), addition of brefeldin A (BFA) (which inhibits AP-1 recruitment) was reported not to block subsequent constitutive-like secretion. To further explore post-TGN trafficking in pancreatic beta-cell lines, we have followed the fate of pulse-labeled procathepsin B (ProB, a lysosomal proenyzme) after postpulse wortmannin treatment or the BFA treatment described above. We find that continuous wortmannin treatment allows ProB to reach immature secretory granules but inhibits its egress from maturing granules. Remarkably, BFA treatment causes augmented unstimulated secretion of newly synthesized ProB that is not paralleled by insulin. This effect requires a delay of 25-35 min after release from the 20 degrees C block. Further, when ProB delivery to endosomes is inhibited, its BFA-augmented secretion is eliminated. We hypothesize that the constitutive-like pathway involves an endosomal intermediate.
Asunto(s)
Endosomas/metabolismo , Islotes Pancreáticos/metabolismo , Proteínas/metabolismo , Androstadienos/farmacología , Animales , Transporte Biológico , Brefeldino A/farmacología , Catepsina B/metabolismo , Precursores Enzimáticos/metabolismo , Insulina/metabolismo , Islotes Pancreáticos/efectos de los fármacos , Islotes Pancreáticos/ultraestructura , Lisosomas/enzimología , Ratas , WortmaninaRESUMEN
Over the past two years, the use of in vitro systems and the identification of autoantibodies to Golgi proteins have provided important new tools for analyzing vesicle and cargo trafficking in the distal secretory pathway. In addition, the phenotypic characterization of mice with knockouts of various prohormone convertases has led to significant progress in understanding the biological relevance of prohormone processing in post-Golgi compartments.
Asunto(s)
Aparato de Golgi/metabolismo , Procesamiento Proteico-Postraduccional , Proteínas/metabolismo , Animales , Transporte Biológico , Gránulos Citoplasmáticos , Modelos Animales de Enfermedad , Endopeptidasas/metabolismo , Humanos , Ratones , Síndrome Oculocerebrorrenal/metabolismoRESUMEN
For several secretory proteins, it has been hypothesized that disulfide-bonded loop structures are required for sorting to secretory granules. To explore this hypothesis, we employed dithiothreitol (DTT) treatment in live pancreatic islets, as well as in PC-12 and GH(4)C(1) cells. In islets, disulfide reduction in the distal secretory pathway did not increase constitutive or constitutive-like secretion of proinsulin (or insulin). In PC-12 cells, DTT treatment caused a dramatic increase in unstimulated secretion of newly synthesized chromogranin B (CgB), presumably as a consequence of reducing the single conserved chromogranin disulfide bond (E. Chanat, U. Weiss, W. B. Huttner, and S. A. Tooze. EMBO J. 12: 2159-2168, 1993). However, in GH(4)C(1) cells that also synthesize CgB endogenously, DTT treatment reduced newly synthesized prolactin and blocked its export, whereas newly synthesized CgB was routed normally to secretory granules. Moreover, on transient expression in GH(4)C(1) cells, CgA and a CgA mutant lacking the conserved disulfide bond showed comparable multimeric aggregation properties and targeting to secretory granules, as measured by stimulated secretion assays. Thus the conformational perturbation of regulated secretory proteins caused by disulfide disruption leads to consequences in protein trafficking that are both protein and cell type dependent.
Asunto(s)
Cromograninas/química , Cromograninas/metabolismo , Disulfuros/química , Insulina/química , Insulina/metabolismo , Proinsulina/química , Proinsulina/metabolismo , Animales , Línea Celular/efectos de los fármacos , Línea Celular/metabolismo , Gránulos Citoplasmáticos/metabolismo , Ditiotreitol/farmacología , Secreción de Insulina , Islotes Pancreáticos/efectos de los fármacos , Islotes Pancreáticos/metabolismo , Ratones , Ratones Endogámicos , Células PC12/efectos de los fármacos , Células PC12/metabolismo , RatasRESUMEN
Polarized trafficking signals may be interpreted differently in different cell types. In this study, we have compared the polarized trafficking of different proteins expressed endogenously in primary porcine thyroid epithelial cells to similar proteins expressed in MDCK cells. As in MDCK cells, NH4Cl treatment of filter-grown thyrocytes caused mis-sorted soluble proteins to exhibit enhanced secretion to the apical medium. In independent studies, thrombospondin 1 (a thyroid basolaterally secreted protein) was secreted basolaterally from MDCK cells. Likewise, the 5'-deiodinase (a thyroid basolateral membrane protein) encoded by the DIO1 gene was also distributed basolaterally in transfected MDCK cells. Consistent with previous reports, when the secretion of human growth hormone (an unglycosylated regulated secretory protein) was examined from transfected MDCK cells, the release was nonpolarized. However, transfected thyrocytes secreted growth hormone apically in a manner dependent upon zinc addition. Moreover, two additional regulated secretory proteins expressed in thyrocytes, thyroglobulin (the major endogenous glycoprotein) and parathyroid hormone (an unglycosylated protein expressed transiently), were secreted apically even in the absence of zinc. We hypothesize that while cellular mechanisms for interpreting polarity signals are generally similar between thyrocytes and MDCK cells, thyrocytes allow for specialized packaging of regulated secretory proteins for apical delivery, which does not require glycosylation but may involve availability of certain ions as well as appropriate intracellular compartmentation.
Asunto(s)
Células Epiteliales/metabolismo , Proteínas de Saccharomyces cerevisiae , Glándula Tiroides/metabolismo , Animales , Polaridad Celular , Células Cultivadas , ADN/análisis , Proteínas de Unión al ADN/metabolismo , Electroforesis en Gel de Poliacrilamida , Electroporación , Técnica del Anticuerpo Fluorescente , Hormona de Crecimiento Humana/metabolismo , Pruebas de Precipitina , Proteínas/metabolismo , Radioinmunoensayo , Porcinos , Tirotropina/metabolismo , Factores de Tiempo , Factores de Transcripción/metabolismo , TransfecciónRESUMEN
Newly synthesized thyroglobulin (Tg), the major secretory glycoprotein of the thyroid gland, folds and homodimerizes in the endoplasmic reticulum (ER) before its export to the site of iodination, where it serves as the precursor for thyroid hormone synthesis. In families with defective Tg export, affected individuals suffer from a thyroidal ER storage disease characterized by a distended thyrocyte ER containing misfolded Tg, along with induced ER molecular chaperones. Inherited as an autosomal recessive trait, deficient Tg causes congenital hypothyroidism in newborns that, if untreated, results in goiter along with serious cognitive and growth defects. Recently, a similar phenotype has been observed in inbred cog/cog mice, although the precise molecular defect has remained undefined. Here, we have isolated and cloned a full-length 8.5-kb Tg cDNA from cog/cog mice and unaffected isogenic AKR/J mice. Comparison of the complete sequences reveals that cog/cog mice express a Leu-2263 --> Pro missense mutation in the acetylcholinesterase-homology domain of Tg. Heterologous expression studies in COS cells indicate that cog Tg exhibits a severe defect in exit from the ER. Site-directed mutagenesis of cog Tg to convert the single amino acid back to Leu-2263 restores normal Tg secretion. We conclude that the cog mutation in Tg is responsible for this ER storage disease that causes thyroid dyshormonogenesis.
Asunto(s)
Hipotiroidismo Congénito , Bocio/congénito , Bocio/genética , Hipotiroidismo/genética , Tiroglobulina/genética , Acetilcolinesterasa/química , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Transporte Biológico Activo/genética , Células COS , Clonación Molecular , ADN Complementario/genética , Modelos Animales de Enfermedad , Retículo Endoplásmico/metabolismo , Expresión Génica , Bocio/metabolismo , Humanos , Hipotiroidismo/metabolismo , Ratones , Ratones Endogámicos AKR , Ratones Mutantes , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Mutación Puntual , Tiroglobulina/química , Tiroglobulina/metabolismoRESUMEN
Secretory granules are specialized intracellular organelles that serve as a storage pool for selected secretory products. The exocytosis of secretory granules is markedly amplified under physiologically stimulated conditions. While granules have been recognized as post-Golgi carriers for almost 40 years, the molecular mechanisms involved in their formation from the trans-Golgi network are only beginning to be defined. This review summarizes and evaluates current information about how secretory proteins are thought to be sorted for the regulated secretory pathway and how these activities are positioned with respect to other post-Golgi sorting events that must occur in parallel. In the first half of the review, the emerging role of immature secretory granules in protein sorting is highlighted. The second half of the review summarizes what is known about the composition of granule membranes. The numerous similarities and relatively limited differences identified between granule membranes and other vesicular carriers that convey products to and from the plasmalemma, serve as a basis for examining how granule membrane composition might be established and how its unique functions interface with general post-Golgi membrane traffic. Studies of granule formation in vitro offer additional new insights, but also important challenges for future efforts to understand how regulated secretory pathways are constructed and maintained.
Asunto(s)
Gránulos Citoplasmáticos/fisiología , Animales , Exocitosis/fisiología , Aparato de Golgi/fisiología , Membranas Intracelulares/química , Membranas Intracelulares/fisiología , Proteínas de la Membrana/fisiologíaRESUMEN
From the studies described in this review, it is clear that structural information dictates not only the functional properties of exportable proteins, but also their ability to be transported in the intracellular secretory pathway. In ERSDs, the precise nature of the defect determines both the severity of the phenotype and the mode of inheritance. To our knowledge, all genetically inherited ERSDs are attributable to mutations in the coding sequence of exportable proteins; thus far, with the exception of abetalipoproteinemia (see Section IV.D), no mutations in ER chaperones (other than those that scientists have genetically engineered) have been reported as the cause of spontaneous disease. The elevations of ER chaperones in ERSDs may differ between mutations, between tissues, between individual patients, and between different physiological states (i.e., such as before and after hormone replacement therapy) in the same patient. Thus, measurement of ER chaperone levels plays an important diagnostic role, but probably should not be used as the sole basis to classify these illnesses. Moreover, because mutant secretory proteins have been reported to occur in virtually every organ system, ERSDs are more readily classified at the cell biological level, by the responses of the cells that actually synthesize the secretory protein, rather than the hormone deficiency associated with the illness at the end-organ level. With these ideas in mind, we present a schematic view in Fig. 4. According to this schema, all ERSDs begin with ER retention of the affected proteins or their subunits. Mutants may then be divided into two groups: type A, where the biological activity is preserved although the protein is transport-deficient; and type B, where the mutant has no potential for functional activity. Both categories include both recessive and dominant mutations. The primary clinical difference between these two classes is that type A ERSDs may be amenable to therapies designed to down-regulate the quality control of ER export so that potentially functional molecules can escape the ER and reach their intended intracellular destination. In both types of ERSDs, in most cases, the retained mutant protein is efficiently degraded in the ER (subtypes A-I and B-I). In these cases, the predominant, global phenotypes involve the symptoms and signs of hormone deficiency. However, careful biochemical and cell biological studies reveal various abnormalities in glandular function, typically including the elevation of the levels of one or more ER chaperones. As described in Section I.C, such elevations are a consequence of chronic adaptation to the presence of unfolded mutant secretory protein (the synthesis of which is stimulated all the more by endocrine feedback loops). As described in Section III, the elevated chaperones appear to be integrally related to the ER retention as well as perhaps the ERAD process that removes the misfolded proteins. In these cases, the ER compartment may expand, but the secretory cells are likely to survive. In the more unusual subtype II (subtypes B-II and perhaps A-II), the mutant protein exhibits an intrinsic tendency to resist ERAD, creating a potentially dangerous accumulation of indigestible material (Fig. 4). This may be due to the unusual production of novel, protease-resistant protein complexes, or it may be due to the formation of protein assemblies that prevent the reverse translocation of mutant proteins to the cytosol for proteasomal proteolysis. Resistance of untransported mutant protein to ER-associated degradation will predispose to a dominant ERSD (460). In such a case, although the mutant allele could could form oligomeric hybrids with the wild-type allele, complete nonmixing of the normally exported wild-type allele and toxic accumulation of the mutant allele is another distinct scenario that can also produce a dominant mode of inheritance. (ABSTRACT TRUNCATED)
Asunto(s)
Retículo Endoplásmico/metabolismo , Errores Innatos del Metabolismo/metabolismo , Chaperonas Moleculares/metabolismo , Procesamiento Proteico-Postraduccional/fisiología , Glicosilación , Humanos , Pliegue de ProteínaRESUMEN
The occurrence of clathrin-coated buds on immature granules (IGs) of the regulated secretory pathway suggests that specific transmembrane proteins are sorted into these buds through interaction with cytosolic adaptor proteins. By quantitative immunoelectron microscopy of rat endocrine pancreatic beta cells and exocrine parotid and pancreatic cells, we show for the first time that the mannose 6-phosphate receptors (MPRs) for lysosomal enzyme sorting colocalize with the AP-1 adaptor in clathrin-coated buds on IGs. Furthermore, the concentrations of both MPR and AP-1 decline by approximately 90% as the granules mature. Concomitantly, in exocrine secretory cells lysosomal proenzymes enter and then are sorted out of IGs, just as was previously observed in beta cells (Kuliawat, R., J. Klumperman, T. Ludwig, and P. Arvan. 1997. J. Cell Biol. 137:595-608). The exit of MPRs in AP-1/clathrin-coated buds is selective, indicated by the fact that the membrane protein phogrin is not removed from maturing granules. We have also made the first observation of a soluble N-ethylmaleimide-sensitive factor attachment protein receptor, syntaxin 6, which has been implicated in clathrin-coated vesicle trafficking from the TGN to endosomes (Bock, J.B., J. Klumperman, S. Davanger, and R.H. Scheller. 1997. Mol. Biol. Cell. 8:1261-1271) that enters and then exits the regulated secretory pathway during granule maturation. Thus, we hypothesize that during secretory granule maturation, MPR-ligand complexes and syntaxin 6 are removed from IGs by AP-1/clathrin-coated vesicles, and then delivered to endosomes.
Asunto(s)
Clatrina/análisis , Gránulos Citoplasmáticos/química , Proteínas de la Membrana/análisis , Proteínas Tirosina Fosfatasas , Receptor IGF Tipo 2/análisis , Subunidades alfa de Complejo de Proteína Adaptadora , Proteínas Adaptadoras del Transporte Vesicular , Animales , Catepsina B/análisis , Catepsina B/metabolismo , Gránulos Citoplasmáticos/metabolismo , Precursores Enzimáticos/análisis , Precursores Enzimáticos/metabolismo , Aparato de Golgi/química , Aparato de Golgi/ultraestructura , Islotes Pancreáticos/química , Isoproterenol/farmacología , Masculino , Glicoproteínas de Membrana/análisis , Proteínas de Neoplasias/análisis , Páncreas/química , Glándula Parótida/química , Proinsulina/análisis , Proteínas Qa-SNARE , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Receptor IGF Tipo 2/metabolismo , Proteínas Tirosina Fosfatasas Clase 8 Similares a ReceptoresRESUMEN
To examine how binding of BiP (a molecular chaperone of the hsp70 family that resides in the endoplasmic reticulum) influences the conformational maturation of thyroglobulin (Tg, the precursor for thyroid hormone synthesis), we have developed a system of recombinant Tg stably expressed in wild-type Chinese hamster ovary (CHO) cells and CHO-B cells genetically manipulated for selectively increased BiP expression. The elevation of immunoreactive BiP in CHO-B cells is comparable to that seen during the unfolded protein response in the thyrocytes of certain human patients and animals suffering from congenital hypothyroid goiter with defective Tg. However, in CHO-B cells, we expressed Tg containing no mutations that induce misfolding (i.e. no unfolded protein response), so that levels of all other endoplasmic reticulum chaperones were normal. Increased availability of BiP did not accelerate Tg secretion; rather, the export of newly synthesized Tg was delayed. Tg detained intracellularly was concentrated in the endoplasmic reticulum. By coimmunoprecipitation, BiP exhibited enhanced binding to Tg in CHO-B cells. Moreover, two-dimensional gel analysis showed that BiP associated especially well with intracellular Tg containing mispaired disulfide bonds, thought to represent early Tg folding intermediates. An endoplasmic reticulum chaperone of the hsp90 family, GRP94, was also associated in Tg-chaperone complexes. The results suggest that increased binding of BiP to Tg leads to its delayed conformational maturation in the endoplasmic reticulum.
Asunto(s)
Proteínas Portadoras/metabolismo , Retículo Endoplásmico/metabolismo , Proteínas de Choque Térmico , Chaperonas Moleculares/metabolismo , Tiroglobulina/metabolismo , Animales , Transporte Biológico , Células COS , Cricetinae , Reactivos de Enlaces Cruzados , Electroforesis en Gel de Poliacrilamida , Chaperón BiP del Retículo Endoplásmico , Glicosilación , Hexosaminidasas/metabolismo , Immunoblotting , Microscopía Fluorescente , Pliegue de Proteína , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Tiroglobulina/química , Tiroglobulina/genética , TransfecciónRESUMEN
To examine the possibility of independent cytoplasmic/transmembrane domain-based apical sorting, we have investigated paramyxovirus SV5 hemagglutinin-neuraminidase (HN), a type II membrane protein with a small N-terminal signal/anchor region. In SV5-infected Madin-Darby canine kidney (MDCK) cells, >90% of HN is found on the apical surface. We have expressed chimeric proteins in which the N terminus of HN, including its signal/anchor region, is attached to a (normally cytosolic) reporter pyruvate kinase (PK). PK itself expressed immediately downstream from a cleavable signal peptide was converted to a 58-kDa N-linked glycosylated form, which was secreted predominantly (80%) to the basolateral surface of MDCK cells. By contrast, stably expressed PK chimeras, now anchored as type II membrane proteins with either the first 48 or 72 amino acids of HN, received similar N-linked glycosylation, yet exhibited polarized transport with a preferentially (75%) apical distribution. These results suggest that the N-terminal signal/anchor region of HN contains independent sorting information for apical specific targeting in MDCK cells.
Asunto(s)
Proteína HN/metabolismo , Señales de Clasificación de Proteína/metabolismo , Respirovirus/metabolismo , Animales , Línea Celular , Perros , Exocitosis , Proteína HN/genética , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Unión Proteica , Piruvato Quinasa/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Respirovirus/enzimologíaRESUMEN
GRP94 serves as a molecular chaperone in the endoplasmic reticulum (ER). In normal thyrocytes, GRP94 interacts transiently with thyroglobulin (Tg), and in thyrocytes of animals suffering from congenital hypothyroid goiter with defective thyroglobulin, GRP94 and thyroglobulin associate in a protracted fashion. In order explore possible consequences of GRP94 binding, we have studied recombinant nonmutant thyroglobulin expressed in control Chinese hamster ovary (CHO) cells in comparison to that produced in CHO cells genetically manipulated for selectively increased GRP94 expression. Levels of ER chaperones other than GRP94 did not detectably differ, and thyroglobulin achieved transport competence in both kinds of CHO cells. However, increased availability of GRP94 caused the residence time of Tg in the ER to be remarkably prolonged. This was accompanied by a major increase in Tg directly associated with GRP94 and an increase in the ER pool size of Tg. Importantly, co-immunoprecipitation analysis revealed disulfide-linked Tg complexes (previously reported as an early Tg-folding intermediate) especially associated with GRP94. Indeed, non-native Tg, GRP94, and a 78-kDa protein likely to be BiP, appeared in ternary complexes. Under these conditions, GRP94 association appears directly involved in prolongation of Tg folding and export, consistent with a role in quality control in the ER.