Portable Dynamometer-Based Measurement of Pelvic Floor Muscle Force.

OBJECTIVE: In attempts to improve the quality of life of women, continuous projects are sought between rehabilitation intervention and engineering. Using the knowledge of the pelvic floor muscle (PFM) physiology, assessment and training methods are developed to reduce lower urinary tract symptoms such as urinary incontinence. Therefore, this paper covers the design and implementation of a portable vaginal dynamometer. METHODS: A PFM probe is designed, 3D printed, assembled, and tested in ten women to assess its acceptability and usability. The feedback from the usability study is used to optimize the PFM probe design. A vaginal dynamometer is developed based on the designed PFM probe, then tested for linearity, repeatability, hysteresis, noise and heat effect, and power consumption. The variability between the different produced PFM probe prototypes is evaluated. RESULTS: Force measurements are made using a load cell. Wireless communication is performed through a Bluetooth low energy transceiver v5.0, with a corresponding interface on both computer and smartphone. The device operates at a 3.3V supply and achieves a power consumption of 49.5 mW in operating mode. Two PFM probe sizes are designed to accommodate different vaginal hiatus sizes, based on usability study feedback. The proposed system allows the physiotherapist to wirelessly monitor variation in pelvic floor muscle force during assessment and/or training. DISCUSSION/CONCLUSION: The testing results showed that the newly designed system has the potential to measure the PFM function in functional conditions such as the standing position.

A Molecular Imprinted PEDOT CMOS Chip-Based Biosensor for Carbamazepine Detection.

A miniaturized biosensor for carbamazepine (CBZ) detection and quantification was designed, implemented and fabricated. The 1×1 mm2 CMOS chip was packaged and coupled with a 3-electrode electrochemical cell. A complete characterization of the sensor was conducted via two steps: 1) Molecular imprinting of PEDOT polymer sites by cyclic voltammetry (CV) on glassy carbon electrode (GCE) surfaces; and 2) Quantification of CBZ solutions through both CV, and a current peak detection circuitry. The proposed biosensor offered high-selectivity and high-sensitivity to CBZ molecules. Scanning electron microscopy (SEM) was utilized to validate the synthesis of the PEDOT chains. CBZ removal from the imprinted polymer was conducted through soaking the modified GCEs in acetonitrile (ACN). Extraction was then confirmed by ultraviolet-visible (UV-vis) spectroscopy and CV analyzing data from pre- and post-template extraction. Furthermore, in order to characterize the electrodes' response to CBZ levels in phosphate buffered solution (PBS) with [Fe(CN)6]3-/4- as a redox pair/mediator, CV and peak detection was conducted resulting in redox peak currents vs. CBZ concentration graphs. The limits of detection (LOD) and quantification (LOQ) were calculated to be 2.04 and 6.2 µg/mL respectively. Finally, selectivity towards CBZ was validated by studying the effect of valproic acid (VPA) and phenytoin (PHT) on the biosensor's performance. The proposed biosensor is highly sensitive and selective to CBZ molecules, simple to construct and easy to operate.

A Dynamometer-based Wireless Pelvic Floor Muscle Force Monitoring.

This paper covers the design and implementation of a proof of concept for a wireless system measuring pelvic floor muscle forces based on a dynamometer. The proposed device is the main component of a novel assessment tool intended for pelvic floor muscle rehabilitation in women suffering from urinary incontinence. The proposed system allows the physiotherapist to wirelessly monitor variation in pelvic floor muscle forces during assessment or training. Wireless communication is provided by a Bluetooth low energy transceiver and a corresponding interface designed for this purpose. Force measurements are sensed with strain gauge precision sensors operated in a Wheatstone bridge configuration. The designed module consumes 14 mW of power in operating mode. System design and experimental results are reported and discussed.

Comparing Caries Experience between Azadirachta indica Chewing Stick Users and Toothbrush Users among 35-44-Year-Old Rural Population of Southern India.

OBJECTIVES: To compare the caries experience between Azadirachta indica chewing stick users and toothbrush users among 35-44-year-old rural population in Southern India. MATERIALS AND METHODS: This ex post facto research was conducted in the rural parts of two sub-administrative areas of a district in the Southern Indian state of Andhra Pradesh. The sample size for the study was determined to be 400, with 200 subjects in each group. Subjects following indigenous oral hygiene methods were identified using an interviewer-administered questionnaire. After obtaining 200 subjects using A. indica chewing sticks, age, gender, and socioeconomic status matched controls using toothbrush were identified. American Dental Association type III examination was carried out to record caries experience (decayed missing filled teeth (DMFT) Index) after obtaining informed consent and thus obtained data were subjected to statistical analysis using the Statistical Package for the Social Sciences (SPSS) software, version 20. RESULTS: It was observed that the caries experience was more in toothbrush users compared to subjects following indigenous methods (DMFT, 4.38 ± 1.93 vs. 3.54 ± 1.02). Similar results were obtained when the decay component of DMFT index was exclusively compared. No significant difference in the plaque scores and the mean number of filled, missing teeth was observed between the two groups. CONCLUSION: Though conclusive results cannot be drawn from this study about the positive influence of indigenous methods on caries experience, the results emphasize the cardinal need to more thoroughly understand the potential benefits of indigenous methods before dismissing them as retrogressive approaches.

Rare finding of Eustachian tube calcifications with cone-beam computed tomography.

Soft tissue calcification is a pathological condition in which calcium and phosphate salts are deposited in the soft tissue organic matrix. This study presents an unusual calcification noted in the cartilaginous portion of the Eustachian tube. A 67-year-old woman presented for dental treatment, specifically for implant placement, and cone-beam computed tomography (CBCT) was performed. The CBCT scan was reviewed by a board-certified oral and maxillofacial radiologist and revealed incidental findings of 2 distinct calcifications in the cartilaginous portion of the Eustachian tube. To the authors' knowledge, no previous study has reported the diagnosis of Eustachian tube calcification using CBCT. This report describes an uncommon variant of Eustachian tube calcification, which has a significant didactic value because such cases are seldom illustrated either in textbooks or in the literature. This case once again underscores the importance of having CBCT scans evaluated by a board-certified oral and maxillofacial radiologist.

Are different generations of CAD/CAM milling machines capable to produce restorations with similar quality?

BACKGROUND: Different CAD/CAM machines' generation may impact the restoration overall quality. The present study evaluated the marginal fit of CAD/CAM restorations manufactured with different generations of CEREC milling unit systems. MATERIAL AND METHODS: Sixteen typodont teeth were divided into two groups (n=8) according to the machine's generation assigned. These are control group (G1): Cerec AC with Bluecam/Cerec 3 milling unit and (G2): Cerec AC with Bluecam/MC XL Premium Package milling unit. Scanning of the preparation were performed and crowns were milled using the Vita Mark II blocks. Blocks were cemented using epoxy glue on the pulpal floor only and finger pressure applied for 1 min. Upon completion of the cementation step, misfits between the restoration and abutment were measured by microphotography and the silicone replica technique using light body silicon material on Mesial (M) and Distal (D) surfaces. RESULTS: Mean and SDs of marginal gaps in micrometers were: G1/M: 94.90 (±38.52), G1/D: 88.53 (±44.87), G2/M: 85.65 (±29.89), G2/D: 95.28 (±28.13). Two-way ANOVA indicated no significant differences among different groups (P>0.05); surface area (P>0.05) and the interaction (P>0.05). Overall, G2 had greater margin gaps than G1, however, without statistical difference (P>0.05). CONCLUSIONS: Difference in milling unit generation did not significantly affect the marginal fit. Marginal gap means were in the range of the clinical acceptance levels for both generations of Cerec milling units, regardless the teeth site area. Key words:CAD/CAM, margin, ceramics.