Glucono-Delta-Lactone-Induced Alginate Gelation: New Insights into the Effect of the Cross-Linker Carrier Type on the Hydrogel Mechanics.

Physical alginate hydrogels commonly rely on "internal gelation" to introduce the cross-linker, e.g., calcium (Ca(II)) ions. These are released in a homogeneous manner by using a pH-sensitive Ca(II) carrier and glucono-delta-lactone (GDL) as the acidifier. Yet, it remains unclear how the carrier of the cross-linker affects the gelation process and final hydrogel properties. We therefore investigate two internal gelation methods using either Ca(II)-chelating ligand complexes or insoluble Ca(II)-based salts. Ionometry coupled with pH measurements reveals the release process of Ca(II) ions upon acidification, which is well described by simulations using the Hyperquad Simulation and Speciation program. We show that these findings correlate well with the evolution of the mechanical properties of the hydrogels. Although the two pH-triggered gelation methods appear to be similar, we demonstrate their differences in terms of the gelation kinetics and final cross-link density. The nature of the ligand or the salt significantly affects the fraction of the released Ca(II) ions and, hence, the mechanical properties of the final hydrogel for a given GDL concentration. Furthermore, for the first time, we demonstrate the competition between GDL and alginate in binding with Ca(II) ions. This study therefore provides different tools for the efficient formulation of alginate hydrogels.

Finite Element Tissue Strains Computation to Evaluate the Mechanical Protection Provided by a New Bilayer Dressing for Heel Pressure Injuries.

OBJECTIVE: Pressure injuries (PIs) result in an extended duration of care and increased risks of complications for patients. When treating a PI, the aim is to hinder further PI development and speed up the healing time. Urgo RID recently developed a new bilayer dressing to improve the healing of stages 2 and 3 heel PIs. This study aims to numerically investigate the efficiency of this new bilayer dressing to reduce strains around the PI site. METHODS: The researchers designed three finite element models based on the same heel data set to compare the Green-Lagrange compressive and maximal shear strains in models without a PI, with a stage 2 PI, and with a stage 3 PI. Simulations with and without the dressing were computed. Analysis of the results was performed in terms of strain clusters, defined as volumes of tissues with high shear and compressive strains. RESULTS: Decreases in the peak and mean values of strains were low in all three models, between 0% and 20%. However, reduction of the strain cluster volumes was high and ranged from 55% to 68%. CONCLUSIONS: The cluster analysis enables the robust quantitative comparison of finite element analysis. Results suggest that use of the new bilayer dressing may reduce strain around the PI site and that this dressing could also be used in a prophylactic manner. Results should be extended to a larger cohort of participants.

One-Step Generation of Alginate-Based Hydrogel Foams Using CO2 for Simultaneous Foaming and Gelation.

The reliable generation of hydrogel foams remains a challenge in a wide range of sectors, including food, cosmetic, agricultural, and medical applications. Using the example of calcium alginate foams, we introduce a novel foam generation method that uses CO2 for the simultaneous foaming and pH reduction of the alginate solution to trigger gelation. We show that gelled foams of different gas fractions can be generated in a simple one-step process. We macroscopically follow the acidification using a pH-responsive indicator and investigate the role of CO2 in foam ageing via foam stability measurements. Finally, we demonstrate the utility of interfacial rheology to provide evidence for the gelation process initiated by the dissolution of the CO2 from the dispersed phase. Both approaches, gas-initiated gelation and interfacial rheology for its characterization, can be readily transferred to other types of gases and formulations.

Microstructure-driven self-assembly and rheological properties of multi-responsive soft microgel suspensions.

HYPOTHESES: The deformation and swelling ability of microgels is influenced by the crosslinking distribution. Varying microgels microstructure is expected to obtain suspensions with different flow behavior and thereby, different rheological properties. EXPERIMENTS: Different multi-responsive microgels were synthesized using two different crosslinkers and varying their amounts: N,N-methylene bis-acrylamide (MBA) and oligo(ethylene glycol) diacrylate (OEGDA). The rheological results were obtained by zero-shear viscosity and long-time creep measurements on concentrated microgel suspensions Microgel microstructure was analyzed by 1H nuclear magnetic resonance transverse relaxation measurements. FINDINGS: At a constant crosslinking rate, we show that the viscosity of OEGDA-crosslinked microgels diverges at a higher concentration than MBA ones, suggesting a looser shell and less restricted dangling chains at the periphery for the later. By scaling with the effective volume fraction, the viscosity curves of the different microgel suspensions reduce into a single curve and closely follow hard sphere models up to ϕeff < 0.45. The results from creep tests revealed a much higher yield stress for MBA-crosslinked microgels, strengthening the hypothesis of a looser shell for the later. Finally, transverse relaxation (T2) NMR measurements demonstrated that, although all microgels exhibit a core-shell microstructure, MBA samples present a less crosslinked shell corroborating with the rheological results.