COMPARATIVE ANALYSIS OF POSITIVE AND NEGATIVE EXPECTATIONS WITH CONTROL OF VOLITIONAL EFFORT IN YOUNG AND OLD AGES AS RISK FACTORS OF SOCIAL AGING.

An elderly person is the subject of changes and development of the basic conditions of his life. Older patients may experience manifestations of social aging, in which some new areas of social reality remain unexplored for them. This article analyzes dispositional optimism and locus of control, as two personal characteristics closely related to the social and psychological aspects. The aim of the study was to examine the relationship between dispositional optimism and locus of control in young and old people. During the study, 115 elderly respondents from the Gerontological Center were surveyed. The average age was 70 [52-74]. A survey of 120 students of the Krasnoyarsk State Medical University of the first, second, third and fourth years, whose average age was 20 [19-25] was conducted. The majority of elderly patients were classified as having an external locus of control (54%), while 46% had an internal locus of control. Regarding optimism, 20% of older people were optimists, 73% were pessimists, and 7% of respondents had a combination of both optimism and pessimism. The majority of young respondents also fell into the category with an external locus of control (70%), while 30% had an internal locus of control. 73% of young people were optimists, 19% were pessimists, and 8% of respondents had a combination of both optimism and pessimism. In older people, there is an inverse relationship between the indicators - the level of internality is inversely proportional to the level of optimism, which is explained by the expectation that bad events will happen more often in the future than good ones. Accordingly, it is necessary to introduce practical recommendations.

[The bioresonance technology--additional possibility to increase the chicken-meat nutrition].

The bioresonance technology which can help to produce the chicken-meat with more optimum protein and fat correlation and higher content of macro- and micro elements without additional introduction it in the fodder is working out.

[Rotavirus infection in a maternity home based on virological and serological study data]. / Rotavirusnaia infektsiia v rodil'nom dome po dannym virusologicheskikh i serologicheskikh issledovanii.

The studies carried out in a maternity house, employing a set of virological and serological methods, revealed the facts of introduction of rotavirus infection into the maternity house, and followed the further spread of the infection. Epidemiological surveys evaluated the sources of infection (parturients, newborns, medical personnel), means of spread of rotavirus infection, and analysed the epidemiological advantages of wards for mother and newborn baby staying together.

[Stability of glucose-6-phosphate dehydrogenase in human cells cultivated in vitro]. / Izuchenie stabil'nosti gliukozo-6-fosfatderidrogenazy v kletkakh cheloveka, kul'tiviruemykh in vitro.

It was shown that the thermal stability of glucose-6-phosphate dehydrogenase in human diploid cells is much higher than in human heteroploid cell lines HeLa and T-9. The purified enzymes from human diploid cells and from HeLa and T-9 cells possess similar thermal stabilities. Mixing of T-9 extracts with the purified enzyme preparations revealed that the non-stability factors of the dehydrogenase are present in the T-9 extracts. An addition of NADP- and NADPH-containing buffers and crystalline NADP to the heteroploid cell extracts stabilizes the enzyme. The thermal stability of the enzyme from "in vitro" cultivated human cells depends on the concentration of the coenzyme. It was also demonstrated that glucose-6-phosphate dehydrogenase stability in HeLa and T-9 extracts is the same at low concentrations of the coenzyme and after addition of crystalline NADP. However, at NADP concentration of 10(-3) M the enzyme stability in HeLa and T-9 extracts is different. It is assumed that the destabilizing factors are the enzymes possessing the nucleotidases activity, which is different in various cell lines.

[Isolation and properties of glucose-6-phosphate dehydrogenase from human cultivated cells]. / Gliukozo-6-fosfatdegidrogenaza kul'tiviruemykh kletok cheloveka. Vydelenie fermenta.

A new procedure for purification of glucose-6-phosphate dehydrogenase resulting in an electrophoretically homogenous preparation made up of 5.10(8) cells (390 mg of protein) is proposed. The enzyme yield is more than 20%. The molecular weights of a subunit and a native enzyme are 55000 and 220000, respectively. The isoelectric point for the protein lies at 4,8. The kinetics of the enzyme thermal inactivation obey the first order equation with the inactivation rate constant of 6.10(-3) min-1.

[Study of human diploid cell strains for the presence of oncornavirus determinants]. / Issledovanie shtammov diploidnykh kletok cheloveka na prisutstvie determinant onkornavirusov.

The WI-38 strain of human diploid cells (HDC) and two strains, L-58 and L-63 isolated in Moscow Research Institute of Virus Preparations were examined in the course of sequential passages for the presence of primate oncornaviruses and their antigens. In parallel, the tumorigenic capacity of the cells was tested by inoculating them into the cheek pouch tissue of Syrian hamsters. In all cases the examined HDC cultures contained no oncornavirus particles or their antigens and in no case showed any infectious activity determined in the KC-test. Only on one subculture of the L-63 strain cells at the 44th passage was a positive result obtained in immunoprecipitation test with antiserum to the LPV oncornavirus. Cells of the L-58 strain which had undergone 27 and 43 passages showed tumorigenicity in hamster experiments. In subsequent passages of these HDC strains no such properties were found. The regular control for the absence of oncogenic message is a necessary condition for the use of HDC strains in virological practice.

[Stability of glucose-6-phosphate dehydrogenase in aging of human diploid cells and the laboratory mouse]. / Izmenenie stabil'nosti gliukozo-6-fosfat-degidrogenazy pri starenii diploidnykh kletok cheloveka i laboratornykh myshei.

Glucose 6-phosphate dehydrogenase (E. C. 1.1.1.49) was found to be inactivated by 30% for 25 min the incubation at 37 degrees C in extracts of human diploid cells and mouse liver, and the stability of the enzyme was found to reduce in aging of human diploid cells and mouse. The stability of dehydrogenase of mice treated with "geroprotector", 2-ethyl-6-methyl-3-hydroxypyridine, exceeds the control level.

[Various biological properties of diploid cell strains derived from various tissues of human embryo]. / Nekotorye biologicheskie svoistva shtammov dilpoidnykh kletok, poluchennykh iz raznykh tkanei émbriona cheloveka

Some properties of 4 diplod cell strains derived from the lung and skin-muscle tissue of human embryos were studied. Only one strain (L-63) was shown to have a stable karyotype throughout its life span and induced no tumor formation in the cheek pouch of hamsters. Species-specific and group antigens were revealed. In the cell strains of the lung and skin-muscle derivation differences in the distribution of lactate dehydrogenase isoenzymes were observed and found characteristic of the original tissue cells.

[Detection of cell culture tumorigenic activity by heterotransplantation into hamster cheek pouch tissue using immunodepressants]. / Vyiavlenie tumorogennoi aktivnosti kul' tur kletok metodom geterotransplantatsii v tkan' zashchechnogo meshka khomiachkov s primeneniem immunodepressantov

Different strains of diploid cells were studied experimentally in Syrian hamsters by heterotransplantation into cheek pouch tissue for detection of their tumorigenic activity. Cortisone and antilymphocyte serum were used as immunodepressors. The results showed that the use of antilymphocyte serum increased considerably the sensitivity of the method of determination of cell culture tumorigenic activity.