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1.
J Hosp Infect ; 99(1): 68-74, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29432820

RESUMEN

BACKGROUND: Cutaneous mucormycoses, mainly due to Lichtheimia (Absidia), have occurred on several occasions in the Burn Unit of the University Hospital of Lille, France. AIM: To investigate the potential vector role of non-sterile bandages used to hold in place sterile gauze used for wound dressing. METHODS: Mycological analysis by conventional culture, Mucorales real-time polymerase chain reaction (qPCR), and Lichtheimia species-specific qPCR were performed on eight crepe and six elasticized bandages that were sampled on two independent occasions in March 2014 and July 2016. Characteristics of the seven Lichtheimia mucormycoses which occurred in burn patients between November 2013 and July 2016 were also collected to assess the epidemiological relationship between potentially contaminated bandages and clinical infections. FINDINGS: One Lichtheimia corymbifera strain was isolated from a crepe bandage by culture, and Lichtheimia spp. qPCR was positive in six out of eight crepe and four out of six elasticized bandages. Using species-specific qPCR, Lichtheimia ramosa, Lichtheimia ornata, and L. corymbifera were identified in six out of ten, five out of ten, and four out of ten bandages, respectively. In patients with mucormycosis, L. ramosa and L. ornata were present in five and two cases, respectively. CONCLUSION: Our data support the utility of Mucorales qPCR for epidemiological investigations, the potential role of these bandages in cutaneous mucormycoses in burn patients in our centre, and, consequently, the need for sterile bandages for the dressing of extensive wounds.


Asunto(s)
Vendajes/microbiología , Quemaduras/complicaciones , Dermatomicosis/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Mucorales/aislamiento & purificación , Mucormicosis/diagnóstico , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Adulto , Anciano , Dermatomicosis/microbiología , Femenino , Francia , Hospitales Universitarios , Humanos , Masculino , Persona de Mediana Edad , Mucorales/genética , Mucormicosis/microbiología
2.
Biomed Chromatogr ; 16(3): 199-202, 2002 May.
Artículo en Inglés | MEDLINE | ID: mdl-11920945

RESUMEN

Ischemia-reperfusion syndrome is a condition where the role of oxygen free radicals is important. Malondialdehyde (MDA) and formaldehyde (FA), products of lipid peroxidation, are the presumptive markers for the development of oxidative stress in tissues and plasmas. A GC-MS method for the determination of MDA and FA in rat brain extract is described. Rat brains were homogenized with deionized water. The homogenates were derivatized with 2,4-dinitrophenylhydrazone (DNPH) to obtain hydrazines derivatives of MDA and FA. The hydrazine derivatives were analyzed by GC-MS and quantitation was by single ion monitoring (SIM). The retention times of FA and MDA were, respectively, 13.75 and 14.20 min, and for SIM quantitation, ion at m/z 210 for FA, and m/z 158 for MDA were used. The results showed that it is possible to estimate the products of lipid peroxidation in brain and to monitor the oxidative stress developed during the ischemia-reperfusion syndrome compared to the normal values.


Asunto(s)
Formaldehído/metabolismo , Cromatografía de Gases y Espectrometría de Masas/métodos , Malondialdehído/metabolismo , Estrés Oxidativo , Animales , Encéfalo/metabolismo , Masculino , Ratas , Ratas Sprague-Dawley , Estándares de Referencia , Reproducibilidad de los Resultados
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