UVB-emitting LEDs for reptile lighting: Identifying the risks of nonsolar UV spectra.

UVB lamps are used to provide reptiles housed indoors with the UV radiation necessary to synthesize vitamin D3 in their skin. Since 2019, UVB-LED lamps have been on sale for use in reptile husbandry. We performed spectral analysis and mapped the UV irradiance for 18 of these lamps. The positive benefits of UVB-LED lamps over traditional products include greater energy efficiency, freedom from mercury and easy installation without external ballasts. However, the spectra of all the UVB-LED lamps tested had little similarity to the solar UV spectrum. Some lamps emitted short-wavelength, non-terrestrial, radiation known to cause acute photo-kerato-conjunctivitis; we report one case. All lamps were lacking significant output in the range 315-335 nm, essential for natural self-regulation of cutaneous vitamin D3 synthesis, preventing overproduction. We describe a possible risk of serious hypervitaminosis D based on our spectral analysis. We call for long-term animal studies to assess this risk, in which the reptiles under these lamps are exposed to species-appropriate UV index levels according to their Ferguson Zone allocation and serum levels of vitamin D3 and 25(OH)D3 monitored. Spectral modifications of the lamps to make the spectrum more like sunlight may be an essential way of mitigating this risk.

Full Spectrum Lighting Induces Behavioral Changes and Increases Cortisol Immunoreactivity in Captive Arachnids.

The use of full spectrum illumination, including ultraviolet (UV), during captive husbandry of arachnids is common practice. The effect of this on captive arachnids has not been previously investigated. Comparison of key behavioral changes and hemolymph cortisol immunoreactivity was undertaken with and without full spectrum lighting. King baboon spiders, Pelinobius muticus and Indian giant scorpions, Heterometrus swammerdami were selected for the study. Both organisms spent all their time hidden when exposed to full spectrum light compared to low-level ambient light except for one instance. There was no significant difference in burrowing and webbing in P. muticus when exposed to full spectrum lighting. There was a decrease in the number of behaviors or postures expressed in full spectrum lighting compared to ambient light for both species. Cortisol immunoactivity of both species were significantly elevated after exposure to full spectrum lighting. This study provides the first evidence of detectable cortisol immunoactivity in arachnid hemolymph. These levels changed in response to full spectrum illumination and were linked to behavioral changes. This suggests that a common husbandry practice may be detrimental to arachnids.

Ultraviolet radiation changes plant color.

BACKGROUND: Plant absorption of ultraviolet (UV) radiation can result in multiple deleterious effects to plant tissues. As a result, plants have evolved an array of strategies to protect themselves from UV radiation, particularly in the UV-B range (280-320 nm). A common plant response to UV exposure is investment in phenolic compounds that absorb damaging wavelengths of light. However, the inverse phenomenon - plant reflectance of UV to protect plant tissues - has not previously been explored. In a paired experiment, we expose half of our sample (N = 108) of insect-pollinated plants of the cultivar Zinnia Profusion Series to UV radiation, and protect the other half from all light < 400 nm for 42 days, and measure leaf and flower reflectance using spectroscopy. We compare UV-B reflectance in leaves and flowers at the beginning of the experiment or flowering, and after treatment. RESULTS: We find that plants protected from UV exposure downregulate UV-B reflectance, and that plants exposed to increased levels of UV show trends of increased UV-B reflectance. CONCLUSIONS: Our results indicate that upregulation of UV-B reflecting pigments or structures may be a strategy to protect leaves against highly energetic UV-B radiation.

Meeting ultraviolet B radiation requirements of amphibians in captivity: a case study with mountain chicken frogs (Leptodactylus fallax) and general recommendations for pre-release health screening.

Conservation breeding programmes are a tool used to prevent amphibian extinctions. The husbandry requirements of amphibians are complex. Ongoing research is needed to ensure optimal management of those captive-bred animals destined, in particular, for reintroduction. The UV-B and vitamin D3 requirements of amphibians are largely unknown. Metabolic bone disease has been reported in a number of species. These include the Critically Endangered mountain chicken frog (Leptodactylus fallax) reared in captivity on diets supplemented with a high-calcium multivitamin and mineral supplement containing vitamin D3 but without UV-B provision. Captive-bred L. fallax being reared for reintroduction to Montserrat were provided with UV-B radiation from metamorphosis and were fed on insects supplemented with vitamins and minerals. Overlapping heat, light and UV-B gradients were provided, mimicking what we believe best represents the natural situation and thereby facilitated self-regulation of UV-B exposure. A subset of 10 frogs was periodically radiographed to assess skeletal health. Radiographic bone density and anatomical integrity appeared unremarkable when compared with a wild caught L. fallax. In addition to other routine health-screening, we recommend that radiography be performed to a structured schedule on a subset of all captive-bred and reared amphibians to assess skeletal health and to gauge the appropriateness of captive husbandry. We demonstrate here that, through the appropriate provision of a combination of both UV-B radiation and dietary supplementation, L. fallax can be bred and reared in captivity with healthy skeletal development.

Voluntary exposure of some western-hemisphere snake and lizard species to ultraviolet-B radiation in the field: how much ultraviolet-B should a lizard or snake receive in captivity?

Studies of voluntary exposure to ultraviolet-B (UVB) radiation from the sun in the field were conducted in the southern US and Jamaica for 15 species of lizards and snakes occupying various habitats. Species were sorted into four zones of UVB exposure ranging from a median UV index of 0.35 for zone 1 to 3.1 for zone 4. Guidelines for UVB exposure in captivity of these and species occupying similar light environments are presented. Data for most species were collected during mid-day during the spring breeding season, which appeared to be the time of maximum exposure. For two species of Sceloporus studied more intensively there was significant variation of exposure among times of the day and among seasons. So, all-day studies over the entire active season are necessary to fully understand the pattern of natural exposure for a particular diurnal species. Environmental and body temperature and thermoregulation as well as UVB/vitamin D photoregulation influences exposure to UVB. Regressions allowing the inter-conversion of readings among some meters with different detector sensitivities are presented. Readings of natural sunlight predict the same photobiosynthetic potential for vitamin D as the same reading from artificial sources whose wavelength distribution within the UVB band of the source is comparable to that of sunlight. Research approaches to further increase our understanding of vitamin D and UVB use and requirements for squamate reptiles in captivity are outlined.

Photodermatitis and photokeratoconjunctivitis in a ball python (Python regius) and a blue-tongue skink (Tiliqua spp.).

A male ball python (Python regius) and a female blue tongue skink (Tiliqua spp.) of unknown age were evaluated for anorexia, lethargy, excessive shedding, corneal opacity (python), and weight loss (skink) of approximately three weeks' duration. These animals represented the worst affected animals from a private herpetarium where many animals exhibited similar signs. At necropsy, the python had bilateral corneal opacity and scattered moderate dysecdysis. The skink had mild dysecdysis, poor body condition, moderate intestinal nematodiasis, and mild liver atrophy. Microscopic evaluation revealed epidermal erosion and ulceration, with severe epidermal basal cell degeneration and necrosis, and superficial dermatitis (python and skink). Severe bilateral ulcerative keratoconjunctivitis with bacterial colonization was noted in the ball python. Microscopic findings within the skin and eyes were suggestive of ultraviolet (UV) radiation damage or of photodermatitis and photokeratoconjunctivitis. Removal of the recently installed new lamps from the terrariums of the surviving reptiles resulted in resolution of clinical signs. Evaluation of a sample lamp of the type associated with these cases revealed an extremely high UV output, including very-short-wavelength UVB, neither found in natural sunlight nor emitted by several other UVB lamps unassociated with photokeratoconjunctivitis. Exposure to high-intensity and/or inappropriate wavelengths of UV radiation may be associated with significant morbidity, and even mortality, in reptiles. Veterinarians who are presented with reptiles with ocular and/or cutaneous disease of unapparent cause should fully evaluate the specifics of the vivarium light sources. Further research is needed to determine the characteristics of appropriate and of toxic UV light for reptiles kept in captivity.