Simplifying assessment of dimensions of oral lesions using a syringe and 'impression planimetry' with printer paper.

Two novel methods of measuring the dimensions of oral lesions are described, which would be of help in deciding relative efficacies of different therapeutic agents when used in clinical trials after larger studies quantitatively assessed for inter/intraobserver variability.

Data set on prediction of friction stir welding parameters to achieve maximum strength of AA2014-T6 aluminium alloy joints.

Statistical tools such as design of experiments (DoE), analysis of variance (ANOVA) were used to develop the empirical relationship, to predict the ultimate tensile strength of the joint at the 95% percent confidence level. Response surface graph and contour plots were constructed using response surface methodology (RSM) concept. From this investigation, it is found that the joint fabricated with a tool rotational speed of 1500 rpm, welding speed of 40 mm/min, tool tilt angle of 1.5° and tool shoulder diameter of 6 mm, exhibited maximum tensile strength of 380 MPa.

The role of T-cell reactivity towards the autoantigen α-NAC in atopic dermatitis.

BACKGROUND: A subgroup of patients with atopic dermatitis (AD) produces IgE autoantibodies to human proteins which may be present in inflamed skin and perpetuate cutaneous inflammation. OBJECTIVES: In order to investigate mechanisms of 'autoallergy' for AD we studied T-cell responses to the autoallergen Hom s 2, the human transcriptional coactivator α-nascent polypeptide-associated complex (α-NAC). METHODS: Specific proliferation of blood lymphocytes from 30 patients and 12 healthy control individuals was investigated by flow cytometry. The proliferation of skin- and blood-derived T cells was assessed in limiting-dilution assays. T-cell clones (TCC) were generated from peripheral blood and from biopsies of lesional skin of patients with AD and the phenotype and cytokine patterns were determined. RESULTS: α-NAC-specific T-cell responses were detected in patients and control individuals. α-NAC induced a significantly higher proliferation of CCR4+ (compared with CCR4-) and CLA+ (compared with CLA-) T cells from the circulation. Limiting-dilution assays revealed a high proliferation of blood and skin-infiltrating lymphocytes in the presence of α-NAC compared with control cultures. α-NAC-specific TCC generated from lesional skin of AD predominantly produced interferon-γ and some TCC also produced interleukin-17. The cytokine pattern of α-NAC TCC may contribute to keratinocyte apoptosis and eczema formation in AD. CONCLUSIONS: α-NAC-specific TCC can be generated from blood and lesional skin of patients with AD. These TCC produce not only Th2 but also Th1 cytokines which may explain the Th1 phenotype of inflammation in AD.

A single-blinded case controlled study on effects of cardiopulmonary circulation on hearing during coronary artery bypass grafting.

OBJECTIVES: To evaluate the effect of extra-corporeal (cardiopulmonary) bypass on hearing during coronary artery bypass grafting. DESIGN: Prospective (single-blinded) controlled study. SETTING: District General Hospital. PARTICIPANTS: Fifty-two patients undergoing coronary artery bypass grafting completed this study. Comparison was made between 14 control patients undergoing off-pump coronary artery bypass grafting and 38 study patients undergoing on-pump coronary artery bypass grafting. The age range of patients was 48-81 years, with 50% between 64 and 66 years. MAIN OUTCOME MEASURES: Pure tone audiograms were performed in all patients before and after coronary artery bypass grafting. Data were analysed for any significant difference between pre- and post-operative pure tone audiograms. RESULTS: Mann-Whitney U-test demonstrated no difference between the area generated between mean pre- and post-operative audiograms (P = 0.754). No significant difference between off versus on pump was demonstrated for average differences at 250-500 Hz, 4 kHz, 4-8 kHz and 8 kHz. Wilcoxon matched-pairs signed-rank test demonstrated no difference between right and left ears for each individual frequency. Spearman's test to analyse the effect on vessel number or minutes on bypass pump revealed no significant difference at 4-8 kHz (P = 0.550 for number of vessels and P = 0.276 for minutes on pump.) CONCLUSION: In this study, it was not possible to demonstrate any statistically significant deleterious effect of extra-corporeal (cardiopulmonary) bypass on hearing during coronary artery bypass grafting.