RESUMEN
INTRODUCTION: Gastro-oesophageal reflux disease affects 10-20% of the population, and laparoscopic fundoplication is one management option. As the most frequently accessed video-sharing website, YouTube has become a popular source of information for patients. This study conducted a cross-sectional analysis of the quality and demographics of patient education videos available on YouTube for laparoscopic fundoplication. METHODS: Three searches were performed on YouTube using the phrases 'laparoscopic fundoplication', 'heartburn surgery' and 'reflux operation'. The Health on The Net (HON) code, DISCERN and Journal of the American Medical Association (JAMA) systems were used to score the first 75 results from each query. Information about each video was collected, including number of views, time since posting, number of comments and the author of the video. Relationships between these variables and video quality were investigated. RESULTS: The median number of views was 3,793. The most common author category was videos produced by surgeons. Overall the quality was poor, mean HON score was 2.5/8, mean DISCERN score was 29.3/80 and mean JAMA score was 1.5/4. Surgeon-authored videos scored higher when scored using the HON and JAMA systems. Videos of longer duration scored higher using all three scoring systems. No other factors were found to be associated with video quality. CONCLUSION: The quality of information in YouTube videos on laparoscopic fundoplication is unreliable. Doctors should be aware of this and caution their patients of YouTube's limitations. Further research is needed to develop validated scoring systems for evaluating the quality of patient education videos.
Asunto(s)
Laparoscopía , Medios de Comunicación Sociales , Humanos , Fundoplicación , Estudios Transversales , Fuentes de Información , Grabación en Video , Difusión de la Información/métodos , Reproducibilidad de los ResultadosRESUMEN
El melanoma subungueal es un subgrupo del melanoma acral lentiginoso. Con frecuencia se diagnostica en etapa avanzada, dada la escasez de síntomas; de ahí que la evaluación y el tratamiento oportuno mejoran el pronóstico. Se presenta el caso de un paciente de 44 años de edad, que acude a consulta de Ortopedia con melanoniquia de tres meses de evolución. Había sido tratado en la atención primaria de salud por un hematoma secundario a un trauma directo sobre la falange distal del primer artejo del pie izquierdo. Fue seguido por consulta externa de Ortopedia y, al no experimentar una evolución adecuada, fue evaluado por la Comisión Provincial de Tumores Periféricos. Se le realizó una biopsia por punch (sacabocado), que arrojó el resultado de melanoma subungueal. Se realizó la amputación del primer artejo y del proximal del primer metatarsiano, una vez verificada la no existencia de lesiones metastásicas, con excelente resultado estético y funcional, sin mostrar recidiva en su seguimiento, a dos años de su intervención(AU)
Subungual melanoma is a subgroup of lentiginous acral melanoma. It is often diagnosed in an advanced stage by a shortage of symptoms, so timely evaluation and treatment improve prognosis. This is the case of a 44-year-old patient who goes to orthopedics with melanonichia 3 months of evolution. He had been treated for a bruise secondary to direct trauma to the distal phalanx of the first left foot ailre in primary health care. It was followed by external orthopedic consultation and by not following an adequate evolution was evaluated by the provincial commission of peripheral tumors. He was given a punch biopsy resulting in a subungual melanoma. The amputation of the first artejo and proximal first metatarsal was carried out, once verified the non-existence of metastatic lesions, with excellent aesthetic and functional result, without showing relapse in its follow-up to two years(AU)
Asunto(s)
Humanos , Masculino , Adulto , Enfermedad Catastrófica/clasificación , Melanoma/diagnóstico , Ortopedia/métodos , Signos y Síntomas , Terapéutica , Biopsia , Amputación Quirúrgica/métodos , Melanoma/cirugíaRESUMEN
In this study, we investigate the forensic and population genetics properties of 21 X-chromosome markers (9 X-Alu insertions and 12 X-STRs) in a dataset composed of 716 individuals from 11 Western Mediterranean populations. The high values of combined forensic parameters indicate that this 21 X-loci panel can complement autosomal or uniparental markers in kinship analysis and complex deficient paternity testing in the populations studied. Population analyses revealed a lower differentiation between Western Mediterranean human groups for X-STRs than for X-Alu insertion polymorphisms. Moreover, X-chromosome markers suggest a sex-biased migration rate, confirming the predominance of patrilocality in this area.
Asunto(s)
Cromosomas Humanos X , Etnicidad/genética , Marcadores Genéticos , Genética de Población , Femenino , Genética Forense , Humanos , Masculino , Región Mediterránea/etnologíaRESUMEN
EZ1 Advanced XL (Qiagen) is an instrument designed to purify automatically nucleic acids from a wide variety of samples: different protocols for DNA extraction from forensic samples are pre-loaded into special EZ1 Advanced XL DNA Investigator Cards. The present study focuses on DNA extraction from biological samples (blood, saliva, sperm) mixed with 3 different types of soils (loam, silt, sandy), using a modified version of the Manufacturer standard protocol. In order to create several traces in the soil, we used biological samples (blood, saliva, sperm) from known donors. Quantification data, reliability and trends in STRs typing success rates using two different commercial multiplexes were evaluated. EZ1 modified DNA extraction protocol allows to recover DNA free of inhibitors and in good quantity for downstream applications.
Asunto(s)
ADN/aislamiento & purificación , Genética Forense/instrumentación , Suelo/química , Análisis Químico de la Sangre , Dermatoglifia del ADN , Genética Forense/métodos , Humanos , Masculino , Repeticiones de Microsatélite , Reacción en Cadena de la Polimerasa , Reproducibilidad de los Resultados , Saliva/química , Semen/química , Manejo de EspecímenesRESUMEN
PURPOSE: This study compared the long-term recurrence rates of laparoscopic totally extraperitoneal (TEP) and open inguinal hernia repair in patients from a randomised trial completed in 1994. Laparoscopic inguinal hernia surgery, especially TEP repair, has gained widespread acceptance in recent years. There is still paucity of data on long-term follow-up comparing recurrence rates for open and laparoscopic techniques. This is the first study providing direct long-term comparative data about these techniques. METHODS: A randomised controlled trial was conducted between 1992 and 1994 on patients undergoing a laparoscopic TEP or an open inguinal hernia (Shouldice) repair at our institution. Of the original 104 participants, contemporary follow-up data could be obtained for 98 patients with regards to long-term recurrence. These data were collected with the help of questionnaires, telephone calls and retrieval of case records. Medical records were reviewed for all patients. Data were analysed using a Cox proportional hazards model. RESULTS: There were 7/72 (9.7%) recurrences in the open group and 9/35 (25.7%) recurrences in the laparoscopic group. This difference in recurrence rates was statistically significant (HR = 2.94; 95% CI 1.05-8.25; p = 0.041.) CONCLUSION: Laparoscopic TEP inguinal hernia repair performed in 1992-1994 had a higher recurrence rate than open Shouldice inguinal hernia repair during the same period. The original study was undertaken in the inceptive days of laparoscopic surgery and results need to be interpreted considering the technology and expertise available at that time.
Asunto(s)
Hernia Inguinal/cirugía , Herniorrafia/efectos adversos , Herniorrafia/métodos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Estudios de Seguimiento , Humanos , Laparoscopía , Masculino , Persona de Mediana Edad , Peritoneo/cirugía , Recurrencia , Adulto JovenRESUMEN
Fundamento: el SIT resulta un síndrome poco frecuente sin ser una rareza. Su origen causal aún es incierto, se le considera debido a la expresión de un gen autosómico recesivo, con incidencia igual en ambos sexos y sin mostrar predilección racial. Está caracterizado por la posición invertida de los órganos en las cavidades torácica y abdominal con respecto al plano sagital, dando la imagen anatómica de las vísceras en espejo. Objetivo: presentar el caso de una paciente con ambas enfermedades asociadas que resulta poco comunes y de interés médico. Caso clínico: paciente normolínea, de piel blanca, femenina y de 38 años de edad, con buena salud anterior a pesar de padecer de una importante anomalía congénita. Llevaba seis meses con síntomas de dispepsia y cólicos biliares a repetición del abdomen izquierdo que se diagnosticó por el método clínico, ultra sonografía y radiología de padecer litiasis vesicular única, donde se confirmó el situs inversus, asociación que es infrecuente. Se interconsulta con cardiología y anestesia, previo consentimiento informado, se realiza colecistectomía abierta con buena evolución hasta su egreso. Conclusiones: la evolución en el intra operatorio y luego en el posoperatorio inmediato y mediato fue buena, a los seis meses del tratamiento está asintomática y con aumento de peso corporal.
Background: a brief revision about two medical subjects was made: totalis situs inversus and gallstone disease and its clinical characteristics, as well as its regular treatment that includes the video-assisted cholecystectomy and the traditional one. A references has been made about this clinical association found in a woman with this congenital anomaly since her childhood, the totalis situs inversus. Objective: to present this patient with both associated deceases that are uncommon and of medical interest. Clinical case: a regular patient was treated, white skin color, female, 38 years old, with good previous health condition in spite of suffering of a major congenital abnormality. She was six month suffering symptoms of dyspepsia and biliary colic repeated in the left abdomen and diagnosed with only gallstone by clinical method, ultrasonography and radiology, getting confirmed this way the totalis situs inversus, association that is uncommon. An interconsultation with Cardiology and Anesthesiology was made, and getting previously the informed consent, an open cholecystectomy was performed with good evolution until her final discharge. Conclusions: the evolution in the intraoperative and later in the immediate and mediate postoperative was good, and after six month of treatment, the patient is asymptomatic and has gained body weight.
RESUMEN
Fundamento: el SIT resulta un síndrome poco frecuente sin ser una rareza. Su origen causal aún es incierto, se le considera debido a la expresión de un gen autosómico recesivo, con incidencia igual en ambos sexos y sin mostrar predilección racial. Está caracterizado por la posición invertida de los órganos en las cavidades torácica y abdominal con respecto al plano sagital, dando la imagen anatómica de las vísceras en espejo.Objetivo: presentar el caso de una paciente con ambas enfermedades asociadas que resulta poco comunes y de interés médico.Caso clínico: paciente normolínea, de piel blanca, femenina y de 38 años de edad, con buena salud anterior a pesar de padecer de una importante anomalía congénita. Llevaba seis meses con síntomas de dispepsia y cólicos biliares a repetición del abdomen izquierdo que se diagnosticó por el método clínico, ultra sonografía y radiología de padecer litiasis vesicular única, donde se confirmó el situs inversus, asociación que es infrecuente. Se interconsulta con cardiología y anestesia, previo consentimiento informado, se realiza colecistectomía abierta con buena evolución hasta su egreso.Conclusiones: la evolución en el intra operatorio y luego en el posoperatorio inmediato y mediato fue buena, a los seis meses del tratamiento está asintomática y con aumento de peso corporal(AU)
Background: a brief revision about two medical subjects was made: totalis situs inversus and gallstone disease and its clinical characteristics, as well as its regular treatment that includes the video-assisted cholecystectomy and the traditional one. A references has been made about this clinical association found in a woman with this congenital anomaly since her childhood, the totalis situs inversus.Objective: to present this patient with both associated deceases that are uncommon and of medical interest.Clinical case: a regular patient was treated, white skin color, female, 38 years old, with good previous health condition in spite of suffering of a major congenital abnormality. She was six month suffering symptoms of dyspepsia and biliary colic repeated in the left abdomen and diagnosed with only gallstone by clinical method, ultrasonography and radiology, getting confirmed this way the totalis situs inversus, association that is uncommon. An interconsultation with Cardiology and Anesthesiology was made, and getting previously the informed consent, an open cholecystectomy was performed with good evolution until her final discharge.Conclusions: the evolution in the intraoperative and later in the immediate and mediate postoperative was good, and after six month of treatment, the patient is asymptomatic and has gained body weight(AU)
Asunto(s)
Humanos , Femenino , Adulto , Colecistectomía , Colelitiasis/cirugía , Colelitiasis/terapia , Situs InversusRESUMEN
Fundamento: el quiste broncógeno mediastinal puro suele localizarse en el mediastino medio, aparece en ambos sexos y a cualquier edad, aunque por ser congénito es mayor en niños y jóvenes, por lo general son asintomáticos y su diagnóstico muchas veces es casual. Se realiza una introducción temática sobre esta tumoración en cuanto a su origen, cuadro clínico, diagnóstico y tratamiento. Se señala que pueden aparecer en otras partes del tórax y cuello. Objetivo: exponer las diferentes formas de presentación del quiste broncógeno mediastinal. Casos clínicos: tres pacientes mujeres, mayores de 45 años atendidas en los servicios de cirugía de los hospitales Amalia Simoni y Manuel Ascunce Domenech a las cuales se le extirpó una tumoración quistíca mediastinal y que fueron estudiadas con anteriodad mediante el análisis en su conjunto del cuadro clínico, la radiografía, ultrasonografía y tomografía axial computarizada del tórax y confirmado el diagnóstico de quiste broncógeno por estudio histopatológico. El tratamiento incluyó resección del tumor mediante toracotomía con buena evolución postoperatoria en todas las enfermas. Conclusiones: se presentan tres enfermas mayores de 45 años tratadas con resección de un quiste broncógeno mediastinal por toracotomía abierta, con buenos resultados terapéuticos.
Background: pure mediastinal bronchogenic cyst is usually located in the middle mediastinum. It is more frequent in both sexes and at any age, although as it is congenital its incidence is higher in children and teenagers. Generally these cysts are asymptomatic and their diagnoses are casual in a lot of cases. A thematic introduction about a mediastinal cystic tumor was performed taking into account its origin, clinical manifestations, diagnosis and treatment. It has been pointed that it might appear in other parts of thorax and neck. Objective: to show the different forms of appearance of mediastinal bronchogenic cyst. Clinical cases: three women over 45 years old were treated in the surgical services areas of Amalia Simoni and Manuel Ascunce Domenech hospitals, to whom a resection of the mediastinal cystic tumor was performed. They were studied in advance through the analysis of the symptoms, thorax radiography, ultrasonography, computerized axial tomography and then the diagnosis was confirmed with the histopathological study. The treatment included tumor excision through thoracotomy with good post-operatory evolution in all the patients. Conclusions: three patients older over 45 years old were treated with mediastinal cystic tumor excision through open thoracotomy with good therapeutic results.
RESUMEN
Fundamento: el quiste broncógeno mediastinal puro suele localizarse en el mediastino medio, aparece en ambos sexos y a cualquier edad, aunque por ser congénito es mayor en niños y jóvenes, por lo general son asintomáticos y su diagnóstico muchas veces es casual. Se realiza una introducción temática sobre esta tumoración en cuanto a su origen, cuadro clínico, diagnóstico y tratamiento. Se señala que pueden aparecer en otras partes del tórax y cuello.Objetivo: exponer las diferentes formas de presentación del quiste broncógeno mediastinal.Casos clínicos: tres pacientes mujeres, mayores de 45 años atendidas en los servicios de cirugía de los hospitales Amalia Simoni y Manuel Ascunce Domenech a las cuales se le extirpó una tumoración quistíca mediastinal y que fueron estudiadas con anteriodad mediante el análisis en su conjunto del cuadro clínico, la radiografía, ultrasonografía y tomografía axial computarizada del tórax y confirmado el diagnóstico de quiste broncógeno por estudio histopatológico. El tratamiento incluyó resección del tumor mediante toracotomía con buena evolución postoperatoria en todas las enfermas.Conclusiones: se presentan tres enfermas mayores de 45 años tratadas con resección de un quiste broncógeno mediastinal por toracotomía abierta, con buenos resultados terapéuticos(AU)
Background: pure mediastinal bronchogenic cyst is usually located in the middle mediastinum. It is more frequent in both sexes and at any age, although as it is congenital its incidence is higher in children and teenagers. Generally these cysts are asymptomatic and their diagnoses are casual in a lot of cases. A thematic introduction about a mediastinal cystic tumor was performed taking into account its origin, clinical manifestations, diagnosis and treatment. It has been pointed that it might appear in other parts of thorax and neck.Objective: to show the different forms of appearance of mediastinal bronchogenic cyst.Clinical cases: three women over 45 years old were treated in the surgical services areas of Amalia Simoni and Manuel Ascunce Domenech hospitals, to whom a resection of the mediastinal cystic tumor was performed. They were studied in advance through the analysis of the symptoms, thorax radiography, ultrasonography, computerized axial tomography and then the diagnosis was confirmed with the histopathological study. The treatment included tumor excision through thoracotomy with good post-operatory evolution in all the patients.Conclusions: three patients older over 45 years old were treated with mediastinal cystic tumor excision through open thoracotomy with good therapeutic results(AU)
Asunto(s)
Humanos , Femenino , Adulto , Persona de Mediana Edad , Quiste Broncogénico , Enfermedades del Mediastino , Quiste Broncogénico/cirugíaRESUMEN
Since 1992, the Spanish and Portuguese-Speaking Working Group of the ISFG (GHEP-ISFG) has been organizing annual Intercomparison Exercises (IEs) coordinated by the Quality Service at the National Institute of Toxicology and Forensic Sciences (INTCF) from Madrid, aiming to provide proficiency tests for forensic DNA laboratories. Each annual exercise comprises a Basic (recently accredited under ISO/IEC 17043: 2010) and an Advanced Level, both including a kinship and a forensic module. Here, we show the results for both autosomal and sex-chromosomal STRs, and for mitochondrial DNA (mtDNA) in two samples included in the forensic modules, namely a mixture 2:1 (v/v) saliva/blood (M4) and a mixture 4:1 (v/v) saliva/semen (M8) out of the five items provided in the 2014 GHEP-ISFG IE. Discrepancies, other than typos or nomenclature errors (over the total allele calls), represented 6.5% (M4) and 4.7% (M8) for autosomal STRs, 15.4% (M4) and 7.8% (M8) for X-STRs, and 1.2% (M4) and 0.0% (M8) for Y-STRs. Drop-out and drop-in alleles were the main cause of errors, with laboratories using different criteria regarding inclusion of minor peaks and stutter bands. Commonly used commercial kits yielded different results for a micro-variant detected at locus D12S391. In addition, the analysis of electropherograms revealed that the proportions of the contributors detected in the mixtures varied among the participants. In regards to mtDNA analysis, besides important discrepancies in reporting heteroplasmies, there was no agreement for the results of sample M4. Thus, while some laboratories documented a single control region haplotype, a few reported unexpected profiles (suggesting contamination problems). For M8, most laboratories detected only the haplotype corresponding to the saliva. Although the GHEP-ISFG has already a large experience in IEs, the present multi-centric study revealed challenges that still exist related to DNA mixtures interpretation. Overall, the results emphasize the need for further research and training actions in order to improve the analysis of mixtures among the forensic practitioners.
Asunto(s)
Cromosomas Humanos X , Cromosomas Humanos Y , Dermatoglifia del ADN , ADN Mitocondrial/genética , Laboratorios/normas , Repeticiones de Microsatélite , Amelogenina/genética , Análisis Químico de la Sangre , Femenino , Genética Forense , Marcadores Genéticos , Haplotipos , Humanos , Masculino , Saliva/química , Semen/químicaRESUMEN
Se trata de una paciente femenina de 70 años, con antecedentes patológicos personales de asma bronquial, hipertensión arterial y diabetes mellitus tipo 2, con insuficiencia renal crónica estadio V secundaria a la diabetes, múltiples ingresos por cuadro de sepsis respiratoria y necesidad de diálisis peritoneal. Se constató esplenomegalia gigante en estudio imagenológico durante uno de los ingresos. Evolucionó desfavorablemente con hipotensión, fiebre y dolor abdominal difuso. Los hallazgos anatomopatológicos mostraron un linfoma de linfocitos pequeños/leucemia linfocítica crónica, estadio IV, con infiltración a múltiples vísceras: ganglios linfáticos, hígado, bazo, pulmón, riñones y serosas. La presencia de pseudofolículos se considera un rasgo morfológico casi absolutamente patognomónico de la enfermedad(AU)
Asunto(s)
Humanos , Leucemia Linfocítica Crónica de Células BRESUMEN
Recently introduced rapidly mutating Y-chromosomal short tandem repeat (RM Y-STR) loci, displaying a multiple-fold higher mutation rate relative to any other Y-STRs, including those conventionally used in forensic casework, have been demonstrated to improve the resolution of male lineage differentiation and to allow male relative separation usually impossible with standard Y-STRs. However, large and geographically-detailed frequency haplotype databases are required to estimate the statistical weight of RM Y-STR haplotype matches if observed in forensic casework. With this in mind, the Italian Working Group (GEFI) of the International Society for Forensic Genetics launched a collaborative exercise aimed at generating an Italian quality controlled forensic RM Y-STR haplotype database. Overall 1509 male individuals from 13 regional populations covering northern, central and southern areas of the Italian peninsula plus Sicily were collected, including both "rural" and "urban" samples classified according to population density in the sampling area. A subset of individuals was additionally genotyped for Y-STR loci included in the Yfiler and PowerPlex Y23 (PPY23) systems (75% and 62%, respectively), allowing the comparison of RM and conventional Y-STRs. Considering the whole set of 13 RM Y-STRs, 1501 unique haplotypes were observed among the 1509 sampled Italian men with a haplotype diversity of 0.999996, largely superior to Yfiler and PPY23 with 0.999914 and 0.999950, respectively. AMOVA indicated that 99.996% of the haplotype variation was within populations, confirming that genetic-geographic structure is almost undetected by RM Y-STRs. Haplotype sharing among regional Italian populations was not observed at all with the complete set of 13 RM Y-STRs. Haplotype sharing within Italian populations was very rare (0.27% non-unique haplotypes), and lower in urban (0.22%) than rural (0.29%) areas. Additionally, 422 father-son pairs were investigated, and 20.1% of them could be discriminated by the whole set of 13 RM Y-STRs, which was very close to the theoretically expected estimate of 19.5% given the mutation rates of the markers used. Results obtained from a high-coverage Italian haplotype dataset confirm on the regional scale the exceptional ability of RM Y-STRs to resolve male lineages previously observed globally, and attest the unsurpassed value of RM Y-STRs for male-relative differentiation purposes.
Asunto(s)
Cromosomas Humanos Y , Bases de Datos Genéticas , Haplotipos , Secuencia de Bases , Conducta Cooperativa , Cartilla de ADN , Humanos , Italia , Control de CalidadRESUMEN
The observation that cancer often arises at sites of chronic inflammation has prompted the idea that carcinogenesis and inflammation are deeply interwoven. In fact, the current literature highlights a role for chronic inflammation in virtually all the steps of carcinogenesis, including tumor initiation, promotion and progression. The aim of the present article is to review the current literature on the involvement of chronic inflammation in the initiation step and in the very early phases of tumorigenesis, in a type of cancer where adult stem cells are assumed to be the cells of origin of neoplasia. Since the gastrointestinal tract is regarded as the best-established model system to address the liaison between chronic inflammation and neoplasia, the focus of this article will be on intestinal cancer. In fact, the anatomy of the intestinal epithelial lining is uniquely suited to study adult stem cells in their niche, and the bowel crypt is an ideal developmental biology system, as proliferation, differentiation and cell migration are all distributed linearly along the long axis of the crypt. Moreover, crypt stem cells are regarded today as the most likely targets of neoplastic transformation in bowel cancer. More specifically, the present review addresses the molecular mechanisms whereby a state of chronic inflammation could trigger the neoplastic process in the intestine, focusing on the generation of inflammatory cues evoking enhanced proliferation in cells not initiated but at risk of neoplastic transformation because of their stemness. Novel experimental approaches, based on triggering an inflammatory stimulus in the neighbourhood of adult intestinal stem cells, are warranted to address some as yet unanswered questions. A possible approach, the targeted transgenesis of Paneth cells, may be aimed at 'hijacking' the crypt stem cell niche from a status characterized by the maintenance of homeostasis to local chronic inflammation, with the prospect of initiating neoplastic transformation in that site.
Asunto(s)
Células Madre Adultas/metabolismo , Transformación Celular Neoplásica/patología , Colitis/patología , Neoplasias Colorrectales/patología , Movimiento Celular , Transformación Celular Neoplásica/inmunología , Colitis/inmunología , Neoplasias Colorrectales/inmunología , Humanos , Mucosa Intestinal/citología , Mucosa Intestinal/patología , Células Madre Neoplásicas/metabolismo , Células Madre Neoplásicas/patología , Transducción de Señal , Nicho de Células MadreRESUMEN
Using a stand-alone pentaplex comprising two standard-length short tandem repeats (STRs): D12S391 and D1S1656 plus three mini-STRs: D2S441, D10S1248 and D22S1045, all recently adopted to extend the European Standard Set (ESS) STRs, we have examined the genotyping performance of the new markers in 111 challenging casework samples. Although commercial kits now combine the five new STRs with existing core loci, we found the ESS-pentaplex we developed in-house performed better than both MiniFiler (comprising eight miniaturized STRs) and the NGM kit that includes the new STRs in a 15-marker multiplex. Our findings suggest at least part of the improved sensitivity of recently available ESS STRs can be attributed to the loci themselves as well as applying long-standing, robust primer designs that were first designed for the extended ESS markers by the laboratories that originally developed them. Therefore the ESS-pentaplex provides an ideal adjunct to Identifiler or MiniFiler to allow laboratories to assess the new STRs alongside existing standard loci, measure performance with challenging material and generate population frequency data ahead of a final decision on which additional STRs will extend the reconfigured CODIS core set.
Asunto(s)
Genética Forense/métodos , Técnicas de Genotipaje/métodos , Repeticiones de Microsatélite , Europa (Continente) , HumanosRESUMEN
Se realizó una revisión bibliográfica sobre el tema Materiales de sutura quirúrgica, tanto nacional como internacionalmente, tratando de encontrar la información más actualizada posible, tomando como base un trabajo publicado en una revista nacional en el año 1990 y una revisión bibliográfica nuestra presentada en el hospital Amalia Simoni en 1995. Se revisaron los materiales informativos de algunas casas comerciales y a partir de aquí, se expusieron las clasificaciones conocidas con los productos que la conforman y se ofrecieron las características del producto comercial y del fabricante. Se señalaron las ventajas de los materiales de sutura de origen sintético sobre los naturales que van siendo desplazados en su utilización y preferencia. Existe el interés de ayudar a mejorar el conocimiento sobre este tema a los profesionales de la salud, personal paramédico y en formación al igual que a médicos veterinarios.
A bibliographical review on the topic of surgical suture material, so much national as internationally, trying to find the most up-to-date possible information, taking as base a work published in a national magazine in the year 1990 and a bibliographical review of ours presented in Amalia Simoni hospital in 1995. The informative materials of some business houses are reviewed and starting from here, the well-known classifications are exposed with the products that compose it and offering the characteristics of the commercial product and the manufacturer. The advantages of the suture materials of synthetic origin are pointed out on the natural ones that are displaced in their use and preference. It encourages us the interest of helping to improve the knowledge on this topic to the health professionals and in formation, paramedic personnel as well as to veterinary doctors.
Asunto(s)
Recursos Materiales en Salud , Instrumentos Quirúrgicos , SuturasRESUMEN
Se realizó una revisión bibliográfica sobre el tema Materiales de sutura quirúrgica, tanto nacional como internacionalmente, tratando de encontrar la información más actualizada posible, tomando como base un trabajo publicado en una revista nacional en el año 1990 y una revisión bibliográfica nuestra presentada en el hospital Amalia Simoni en 1995. Se revisaron los materiales informativos de algunas casas comerciales y a partir de aquí, se expusieron las clasificaciones conocidas con los productos que la conforman y se ofrecieron las características del producto comercial y del fabricante. Se señalaron las ventajas de los materiales de sutura de origen sintético sobre los naturales que van siendo desplazados en su utilización y preferencia. Existe el interés de ayudar a mejorar el conocimiento sobre este tema a los profesionales de la salud, personal paramédico y en formación al igual que a médicos veterinarios (AU)
A bibliographical review on the topic of surgical suture material, so much national as internationally, trying to find the most up-to-date possible information, taking as base a work published in a national magazine in the year 1990 and a bibliographical review of ours presented in Amalia Simoni hospital in 1995. The informative materials of some business houses are reviewed and starting from here, the well-known classifications are exposed with the products that compose it and offering the characteristics of the commercial product and the manufacturer. The advantages of the suture materials of synthetic origin are pointed out on the natural ones that are displaced in their use and preference. It encourages us the interest of helping to improve the knowledge on this topic to the health professionals and in formation, paramedic personnel as well as to veterinary doctors (AU)