COVID-19 outbreaks surveillance through text mining applied to electronic health records.

BACKGROUND: The COVID-19 pandemic has caused significant disruptions to everyday life and has had social, political, and financial consequences that will persist for years. Several initiatives with intensive use of technology were quickly developed in this scenario. However, technologies that enhance epidemiological surveillance in contexts with low testing capacity and healthcare resources are scarce. Therefore, this study aims to address this gap by developing a data science model that uses routinely generated healthcare encounter records to detect possible new outbreaks early in real-time. METHODS: We defined an epidemiological indicator that is a proxy for suspected cases of COVID-19 using the health records of Emergency Care Unit (ECU) patients and text mining techniques. The open-field dataset comprises 2,760,862 medical records from nine ECUs, where each record has information about the patient's age, reported symptoms, and the time and date of admission. We also used a dataset where 1,026,804 cases of COVID-19 were officially confirmed. The records range from January 2020 to May 2022. Sample cross-correlation between two finite stochastic time series was used to evaluate the models. RESULTS: For patients with age 18 years, we find time-lag () = 72 days and cross-correlation () ~ 0.82, = 25 days and ~ 0.93, and = 17 days and ~ 0.88 for the first, second, and third waves, respectively. CONCLUSIONS: In conclusion, the developed model can aid in the early detection of signs of possible new COVID-19 outbreaks, weeks before traditional surveillance systems, thereby anticipating in initiating preventive and control actions in public health with a higher likelihood of success.

Repellent activity of the non-host semiochemical (E)-2-octenal against Amblyomma sculptum and Amblyomma dubitatum ticks under field conditions.

Amblyomma ticks pose a significant public health threat due to their potential to transmit pathogens associated with rickettsial diseases. (E)-2-octenal, a compound found in donkeys (Equus asinus), exhibits strong repellent properties against Amblyomma sculptum nymphs under laboratory conditions. This study assessed the effectiveness of the (E)-2-octenal in wearable slow-release devices for personal human protection against Amblyomma ticks under natural conditions. Slow-release devices treated with (E)-2-octenal and untreated controls were prepared and tested on two volunteers walking through a tick-infested area in Goiania, Brazil. The experiment was conducted twice daily for three series of 10 days, with each volunteer wearing two devices attached to each leg, one on the ankle and one just above the thigh. Volunteers with control and treated devices exchanged them between rounds. Also, the daily release rate of (E)-2-octenal from the slow-release devices was determined in the laboratory, increasing significantly from 0.77 ± 0.14 µg/day on the first day to 9.93 ± 1.92 µg/day on the 4th day and remaining constant until the 16th day. A total of 5409 ticks were collected from both volunteers. Treated devices resulted in recovering fewer ticks (n = 1,666; 31%) compared to untreated devices (control: n = 3,743; 69%). (E)-2-octenal effectively repelled Amblyomma spp. larvae, A. sculptum adults, and exhibited pronounced repellency against A. dubitatum nymphs and adults. These findings suggest the potential of (E)-2-octenal delivered by wearable slow-release devices as a green-based repellent. Further improvements, however, are necessary to provide better protection for humans against A. sculptum and A. dubitatum in field conditions.

The C-terminal mutation beyond the catalytic site of brown spider phospholipase D significantly impacts its biological activities.

Loxosceles spider envenomation results in dermonecrosis, principally due to phospholipases D (PLDs) present in the venom. These enzymes have a strongly conserved sequence, 273ATXXDNPW280, in the C-terminal region (SMD-tail) that make contact with ß-sheets of the TIM barrel, in which the amino acids Asp277 and Trp280 establish the energetically strongest contacts. The SMD-tail is conserved in PLDs from different species but absent in the non-toxic PLD ancestral glycerophosphodiester phosphodiesterases (GDPDs). This work aims to understand the role of the C-terminal region in the structural stability and/or function of phospholipases D. Through site-directed mutagenesis of the rLiD1 protein (recombinant Loxosceles intermedia dermonecrotic protein 1), we produced two mutants: rLiD1D277A and rLiD1W280A (both with sphingomyelinase activity), in which Asp277 and Trp280 were replaced by alanine. rLiD1D277A showed similar sphingomyelinase activity but at least 2 times more dermonecrotic activity than rLiD1 (wild-type protein). Conversely, while the rLiD1W280A displayed a slight increase in sphingomyelinase activity, its biological activity was similar or lower compared to rLiD1, potentially due to its decreased thermostability and formation of amyloid aggregates. In conclusion, these new findings provide evidence that SMD-tail mutants impact the structure and function of these proteins and point out that residues outside the active site can even increase the function of these enzymes.

From the laboratory to the field: efficacy of entomopathogenic nematodes to control the cattle tick.

BACKGROUND: The control of ticks is challenged by the resistance of tick populations to chemical acaricides. In this study, we evaluated, under laboratory conditions, the efficacy of Heterorhabditis bacteriophora against Rhipicephalus (Boophilus) microplus engorged females with varying body weights (150, 200, 250, 300 or 350 mg per female) or from eight different geographical populations. We also determined the efficacy of H. bacteriophora for tick control under field conditions. RESULTS: R. microplus engorged females with varying body weights exposed to 150 juveniles of H. bacteriophora resulted in a high control efficacy (97.5% to 98.4%). Tests with females from different geographical populations comprised eight tick strains treated with H. bacteriophora and their respective control groups. The biological parameters of females exposed to nematode treatments did not differ significantly and resulted in 89% to 99% of control efficacy. Trials conducted under field conditions were performed in field plots with Megathyrsus maximus grass. Treatment groups received eight cadavers of Tenebrio molitor fully colonized with H. bacteriophora at 1 week prior to the release of female ticks, whereas control groups were untreated. On the first day of the experiment, six engorged females were distributed in each plot. On day 42 and day 63, the apical portion of the grasses with R. microplus larvae were collected and quantified. The population of R. microplus larvae was reduced up to 73.1% in plots treated with H. bacteriophora at day 63 after treatment. CONCLUSION: R. microplus engorged females with varying body weights or from different geographical populations were highly susceptible to H. bacteriophora. The field test demonstrated the efficacy of H. bacteriophora in reducing R. microplus larvae in infested pastures. © 2022 Society of Chemical Industry.

Quantitative Analysis of Viscoelastic Properties of Red Blood Cells using Optical Tweezers and Defocusing Microscopy.

The viscoelastic properties of erythrocytes have been investigated by a range of techniques. However, the reported experimental data vary. This is not only attributed to the normal variability of cells, but also to the differences in methods and models of cell response. Here, an integrated protocol using optical tweezers and defocusing microscopy is employed to obtain the rheological features of red blood cells in the frequency range of 1 Hz to 35 Hz. While optical tweezers are utilized to measure the erythrocyte-complex elastic constant, defocusing microscopy is able to obtain the cell height profile, volume, and its form factor a parameter that allows conversion of complex elastic constant into complex shear modulus. Moreover, applying a soft glassy rheology model, the scaling exponent for both moduli can be obtained. The developed methodology allows to explore the mechanical behavior of red blood cells, characterizing their viscoelastic parameters, obtained under well-defined experimental conditions, for several physiological and pathological conditions.

An 82 bp tandem repeat family typical of 3' non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres.

Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and good coverage genome sequencing of dozens of other wild Coffea spp., repetitive DNA sequences were identified, and the genomes were compared to decipher particularities of pericentromeric structures. The searches revealed a short tandem repeat (82 bp length) typical of Gypsy/TAT LTR retrotransposons, named Coffea_sat11. This repeat organises clusters with fragments of other transposable elements, comprising regions of non-coding RNA production. Cytogenomic analyses showed that Coffea_sat11 extends from the pericentromeres towards the middle of the chromosomal arms. This arrangement was observed in the allotetraploid C. arabica chromosomes, as well as in its progenitors. This study improves our understanding of the role of the Gypsy/TAT LTR retrotransposon lineage in the organisation of Coffea pericentromeres, as well as the conservation of Coffea_sat11 within the genus. The relationships between fragments of other transposable elements and the functional aspects of these sequences on the pericentromere chromatin were also evaluated. Highlights: A scattered short tandem repeat, typical of Gypsy/TAT LTR retrotransposons, associated with several fragments of other transposable elements, accumulates in the pericentromeres of Coffea chromosomes. This arrangement is preserved in all clades of the genus and appears to have a strong regulatory role in the organisation of chromatin around centromeres.

Efficacy of collars with allomones on dogs to control Rhipicephalus sanguineus sensu lato infestations under field conditions.

Benzaldehyde and 2-hexanone are allomones produced by beagle dogs that reduce infestation by Rhipicephalus sanguineus sensu lato on these animals and on susceptible dogs which artificially release these repellents. These observations were obtained in previous laboratory tests or artificial infestations of susceptible dogs under controlled conditions. Here we evaluated the efficacy of collars delivering these repellents for suppressing the loads of R. sanguineus s.l. on naturally infested mixed-breed dogs under field conditions. Thirty dogs naturally infested with R. sanguineus s.l. were separated into two groups with 15 dogs each. The dogs from the treatment group received the collars with slow-release formulations of the allomones, and the dogs from the control group received collars without such compounds. Collar effectiveness tests were carried out over 30 days. All ticks found were removed from each dog, identified, and counted every collection day. The density of the different life stages of R. sanguineus s.l. varied greatly between groups and across evaluation times. Adult ticks were the stage most abundant and prevalent on infested dogs. The counts for larvae, nymphs and adults were similar between the control and the treatment groups within each tick collection day. Conversely, when we considered the total number of ticks over 30 days and gathered all life stages collected in the same animal, the dogs from the control group had significantly higher loads (35.3 ± 4.27) of ticks than the dogs from the treatment group (21.8 ± 2.96) (P < 0.01). In addition, the efficacy of treatment with repellent collars was high for adult ticks (30.8%) but was extremely low for reducing larval or nymphal infestation (0 or 2.6%, respectively). The present study demonstrates, for the first time, the efficacy of these volatile compounds on naturally infested dogs under field conditions. The development of a technologically enhanced device for slow release of the allomones tested here can be an advantageous alternative for reducing R. sanguineus s.l. infestation on dogs in residential environments.

Selecting 'convenient observers' to probe the atomic structure of CVD graphene on Ir(111) via photoelectron diffraction.

CVD graphene grown on metallic substrates presents, in several cases, a long-range periodic structure due to a lattice mismatch between the graphene and the substrate. For instance, graphene grown on Ir(111), displays a corrugated supercell with distinct adsorption sites due to a variation of its local electronic structure. This type of surface reconstruction represents a challenging problem for a detailed atomic surface structure determination for experimental and theoretical techniques. In this work, we revisited the surface structure determination of graphene on Ir(111) by using the unique advantage of surface and chemical selectivity of synchrotron-based photoelectron diffraction. We take advantage of the Ir 4f photoemission surface state and use its diffraction signal as a probe to investigate the atomic arrangement of the graphene topping layer. We determine the average height and the overall corrugation of the graphene layer, which are respectively equal to 3.40 ± 0.11 Å and 0.45 ± 0.03 Å. Furthermore, we explore the graphene topography in the vicinity of its high-symmetry adsorption sites and show that the experimental data can be described by three reduced systems simplifying the moiré supercell multiple scattering analysis.

Tratamento cirúrgico de fratura nasal: relato de caso / Surgical treatment of nasal fracture in: case report

O trauma em ossos nasais tem a terceira maior incidência de todo o esqueleto, e, em fraturas faciais, são os ossos mais acometidos, estando o arco zigomático em segundo lugar. Os fatores etiológicos comuns do trauma nasal são: acidentes motociclísticos, agressões físicas e acidentes desportivos, sendo o gênero masculino o mais predominante. Na suspeita de trauma nasal, deve-se investigar o histórico de epistaxe, obstrução nasal, dor e assimetria, combinando o exame clínico ao exame de imagem para concluir o diagnóstico. Este trabalho teve como metodologia adotada a revisão de literatura nas bases de dados PUBMED, SCIELO e MedLine, com o objetivo de relatar um caso clínico de uma paciente do sexo feminino, 38 anos, vítima de agressão física, cursando com fratura de ossos próprios do nariz e zigoma sem deslocamento com sinais clínicos de rinoescoliose, epistaxe e sintomas de obstrução nasal e dor em face. Pelas condições apresentadas no exame clínico e de imagem, a proposta de tratamento foi a redução fechada da fratura de nariz, sob anestesia geral, 15 dias após o trauma. Conclusão: O tratamento das fraturas em ossos nasais pode, de acordo com a literatura, ser sob anestesia geral ou local, apresentando bons resultados em ambos, com o mesmo objetivo, o de devolver função e estética e, quando possível, abordar em curto espaço de tempo, em função da rapidez de consolidação óssea da fratura... (AU)

The nasal bones trauma has the third highest incidence of the entire skeleton, and, in facial fractures, are the most affected bones, with the zygomatic arch being in second place. The common etiological factors of nasal trauma are: motorcycle accidents, physical aggressions and sports accidents, with the male gender being the most prevalent. In suspected nasal trauma, the history of epistaxis, nasal obstruction, pain and asymmetry should be investigated, combining clinical examination and imaging to conclude the diagnosis. The methodology used in this study was the literature review in the PUBMED, SCIELO and MedLine databases. The objective of this study is to report a clinical case of a female patient, 38 years old, physical aggression victim, with fracture of bones of the nose - displaced zygoma with clinical signs of rhinoscoliosis, epistaxis and symptoms of nasal obstruction and facial pain. Considering the conditions presented in the clinical and imaging examination, the treatment proposal was the closed reduction of the nose fracture, under general anesthesia, 15 days after the trauma. Conclusion: The treatment of fractures in nasal bones may, according to the literature, be under general or local anesthesia, with good results in both, the objective is the same, to return function and aesthetics and, when possible, to approach in a short period of time due to the rapid bone consolidation of the fracture... (AU)

Displasia cemento-óssea florida: relato de dois casos tratados por diferentes abordagen / Florid cemento-osseous dysplasia: report of two cases treated with different approaches

Objetivo: relatar dois casos de displasia cemento-óssea florida, descrevendo a evolução clínico-radiográfica ao longo de 5 anos em um paciente assintomático e a abordagem cirúrgica em um caso sintomático. Relato de caso 1: paciente, melanoderma, sexo feminino, 56 anos de idade, encaminhada ao Serviço de Cirurgia Bucomaxilofacial da Universidade Federal da Bahia, por cirurgião-dentista clínico que notou alterações imagiológicas em radiografia panorâmica de rotina. Apesar da importante extensão da lesão em maxila e mandíbula, não havia qualquer sintoma ou sinal clínico de infecção. A paciente foi acompanhada durante 5 anos, com exames de imagem bianuais e medidas clínicas profiláticas. Relato de caso 2: paciente, melanoderma, sexo feminino, 57 anos, apresentou-se ao ambulatório de cirurgia queixando-se de atraso em cicatrização após remoção de um dente. A radiografia panorâmica e a tomografia computadorizada, em conjunto com dados clínicos, permitiram o diagnóstico de displasia cemento-óssea florida com infecção secundária. A paciente foi abordada por meio de osteotomia em região do defeito em mandíbula. A análise microscópica do espécime obtido confirmou a alteração displásica cementoide. Os sinais e sintomas regrediram e a paciente segue em acompanhamento. Considerações finais: a displasia cemento-óssea florida, portanto, é uma doença pouco frequente, cujas manifestações podem demandar diferentes abordagens. É importante o domínio clínico do cirurgião-dentista, uma vez que o diagnóstico equivocado pode guiar a escolhas terapêuticas com resultados insatisfatórios. (AU)

Objective: the objective of this article is to report two cases of florid cemento-osseous dysplasia describing the clinical-radiographic evolution over 5 years in an asymptomatic patient and the surgical approach in a symptomatic one. Case report 1: patient, melanoderma, female, 56 years old, referred to the service by a clinical Dentist who noticed imaging alterations in a routine panoramic radiography. Despite the important extension of the maxillary and mandibular lesion, there was no clinical sign or symptom of infection. The patient has been followed for 5 years with biannual imaging exams and prophylactic clinical measures. Case report 2: patient, melanoderma, female, 57 years old, presented to the surgery outpatient complaining of delay in healing after removal of a tooth. Panoramic X-ray and Computed Tomography together with clinical data allowed the diagnosis of florid cemento-osseous dysplasia with secondary infection. The patient was approached through osteotomy in the region of the mandible defect. The microscopic analysis of the specimen confirmed the dysplastic cementenoid alteration. Signs and symptoms regressed and the patient is in follow up. Final considerations: florida cementoosseous dysplasia is an infrequent disease, whose manifestations may require different approaches. The clinical domain of the Dentist is important, since misdiagnosis can lead to therapeutic choices with unsatisfactory results. (AU)

Redução de fratura de arco zigomático sob anestesia local ­ relato de caso / Reduction of zygomatic arch fracture under local anesthesia ­ case report

As fraturas do arco zigomático são geralmente causadas por trauma direto e refletem prejuízos estéticos e funcionais, devendo ser realizado o correto diagnóstico por meio de exame clínico e radiográfico para realizar o tratamento adequado e evitar a presença de sequelas pós-traumáticas. O objetivo desse trabalho é relatar um caso de fratura isolada de arco zigomático, descrever os meios de diagnóstico e tratamento fechado desse tipo de fratura. Paciente HVS, gênero masculino, 38 anos, vítima de agressão física compareceu ao serviço de Cirurgia e Traumatologia Bucomaxilofacial da UFBA/OSID por meio da emergência do Hospital Geral do estado (Salvador/BA) há aproximadamente uma hora após o trauma. Foi realizada a antissepsia com iodo tópico, anestesia local superficial e incisão cutânea com lamina de bisturi nº 15. Após o acesso, realizou-se a introdução do gancho de Barros e redução fechada da fratura. Paralelamente ao relato, foi realizada uma pesquisa na literatura científica para embasar os métodos e técnicas utilizados no diagnóstico e tratamento. Observou-se, então, a partir do caso clínico que é possível reduzir fraturas isoladas de arco zigomático sob anestesia local com acesso próximo à fratura para redução fechada a fim de evitar aumento da morbidade de procedimentos e reduzir a oneração ao serviço público (AU).

Fractures of the zygomatic arch are usually caused by direct trauma and reflect aesthetic and functional damages, and the correct diagnosis must be made by means of clinical and radiographic examination in order to carry out the appropriate treatment and avoid the presence of post-traumatic sequelae. The objective of this work is to report a case of isolated fracture of the zygomatic arch, to describe the means of diagnosis and closed treatment of this type of fracture. Patient HVS, male gender, 38 years old, victim of physical aggression attended the Service of Oral and Maxillofacial Surgery and Traumatology of UFBA/OSID through the emergency of the state general hospital (Salvador/Bahia) approximately one hour after the trauma. Antissepsis was performed with topical iodine, superficial local anesthesia, and cutaneous incision with scalpel blade nº 15. After the access was performed the introduction of the Barros hook and closed reduction of the fracture. Parallel to the report, it has done a research in the scientific literature to support the methods and techniques used in diagnosis and treatment. It is observed from the clinical case that it is possible to reduce zygomatic arch fractures isolated under local anesthesia with close access to closed reduction fracture in order to avoid increased morbidity of procedures and reduce the burden on the public service (AU).

Cisto radicular gigante em maxila: relato de caso / Giant maxillary radicular cyst: case report

The radicular cyst is the most common maxillary inflammatory odontogenic cyst. It has predilection for the anterior region of the maxilla and its occurrence is more frequent in males between the third and the sixth decade. As literature shows few cases of large radicular cysts causing facial asymmetry, this paper aims to document a successful case of definitive treatment by surgical enucleation of a giant radicular cyst in the anterior region of the maxilla. Adult male patient noticed increased volume in the anterior region of the right maxilla, with symptoms, mobility in dental units 11 and 21 and evolution of approximately one year. The panoramic radiography showed an unilocular radiolucent image with oval appearance lined by a discreet radiopaque halo extending to the apex of teeth 11, 12, 13, 21, and 22, suggesting a radicular cyst. Total excision of the lesion and curettage was performed under general anesthesia, and histopathological examination confirmed the diagnosis of the radicular cyst. The patient is under follow-up by the team without any possible expectations of lesion recurrence. The proliferation and activation of epithelial cell rests of Malassez induced by dental caries explains the origin of this cyst. Radicular cysts of large proportions are rare and its growth is due to bone reabsorption and cortical expansion, which can reach noble facial areas and cause asymmetries. The primary treatment of large cysts is enucleation and endodontic treatment of the involved teeth(AU)

O cisto radicular é o cisto odontogênico inflamatório maxilar mais comum. Tem predileção pela região anterior da maxila e sua ocorrência é mais frequente no sexo masculino entre a terceira e a sexta década. Como a literatura mostra poucos casos de grandes cistos radiculares causando assimetria facial, este trabalho tem como objetivo documentar um caso bem-sucedido de tratamento definitivo por enucleação cirúrgica de um cisto radicular gigante na região anterior da maxila. Paciente do sexo masculino adulto observou aumento de volume na região anterior da maxila direita, com sintomas, mobilidade nas unidades odontológicas 11 e 21 e evolução de aproximadamente um ano. A radiografia panorâmica mostrou imagem radiolúcida unilocular de aspecto oval revestida por um discreto halo radiopaco que se estendia até o ápice dos dentes 11, 12, 13, 21 e 22, sugerindo um cisto radicular. A excisão total da lesão e curetagem foi realizada sob anestesia geral e o exame histopatológico confirmou o diagnóstico do cisto radicular. O paciente está em acompanhamento pela equipe sem qualquer expectativa possível de recorrência da lesão. A proliferação e ativação de restos de células epiteliais de Malassez induzidas por cárie dentária explica a origem deste cisto. Cistos radiculares de grandes proporções são raros e seu crescimento é devido à reabsorção óssea e à expansão cortical, que pode atingir áreas nobres da face e causar assimetrias. O tratamento primário de grandes cistos é a enucleação, curetagem e o tratamento endodôntico dos dentes envolvidos(AU)

Manejo cirúrgico de fratura de mandíbula: relato de caso / Surgical management of mandibular fracture: case report

Objetivo: descrever, por meio de um relato de caso, uma fratura de mandíbula em região de parassínfise esquerda, causada com agressão física. Relato de caso: paciente do sexo feminino, 26 anos de idade, ASA I, melanoderma, vítima de agressão física, procurou o serviço de Cirurgia e Traumatologia Bucomaxilofacial da Faculdade de Odontologia da Universidade Federal da Bahia. Ao exame físico, referiu queixas álgicas espontâneas em região mandibular esquerda e presença de degrau ósseo em região passinfisária esquerda. Ao exame de imagem, observaram-se sinais sugestivos de fratura em região de parassínfise esquerda. Na paciente em questão, foi realizado o manejo cirúrgico da fratura com auxílio de miniplacas e parafusos do sistema 2 mm em acesso intraoral. No acompanhamento pós-operatório, observaram-se material de osteossíntese e odontossíntese em posição, fratura adequadamente reduzida e ausência de sinais de deslocamento dos cotos fraturados. Considerações finais: o uso do sistema de fixação interna rígida é indicado em casos de fraturas desfavoráveis de mandíbula, uma vez que apresenta resultados satisfatórios referentes a estabilização e cicatrização óssea. A redução e a fixação com auxílio de miniplacas e parafusos tendem a evitar o deslocamento dos cotos fraturados e a formação de pseudoartrose. (AU)

Objective: to describe, through a case report, a mandible fracture in the left paraphysis region caused by physical aggression. Case report: female patient, 26 years old, ASA I, melanomedma, victim of physical aggression, researched at the Department of Oral and Maxillofacial Surgery and Traumatology from the Federal University of Bahia. In the physical exam, the patient referred spontaneos pain in the left parasymphysis and bone stepping in passinfisary left region. At the imaging examination observed suggestive signs of a fracture in the region of the left parasymphysis. The patient in question was approached by a surgical procedure of the fracture with miniplates and screws of the 2.0 mm system in an intraoral approach. Postoperatively, osteosynthesis and odontosynthesis material was observed in position, adequately reduced fracture and absence of signs of displacement of fractured stumps. Final considerations: the use of the internal fixation system is indicated in cases of unfavorable mandibular fractures once it presents satisfactory results regarding bone stabilization and healing. The reduction and fixation with miniplates and screws tend to avoid the displacement of fractured stumps and formation of pseudoarthrosis. (AU)

Reconstruções orbitárias: relato de três casos / Orbital reconstructions: three case reports

Objetivo: este trabalho visa a apresentar uma série detrês casos clínicos com diferentes condutas para tratamentode fraturas de assoalho de órbita. Relato decasos: no primeiro caso, o paciente, sexo masculino,foi vítima de ferimento por arma de fogo, apresentandodiplopia, trismo e queixas estéticas. A conduta seguidafoi reconstrução do assoalho da órbita com telade titânio isolada. No segundo caso, o paciente, sexomasculino, foi vítima de acidente motociclístico, apresentandodiplopia e enoftalmo. A conduta seguida foireconstrução utilizando associação de tela de titâniocom polimetilmetacrilato (PMMA). No terceiro caso, opaciente, sexo masculino, foi vítima de acidente automobilístico,apresentando enoftalmo, diplopia e perdade projeção malar. A conduta seguida foi reconstruçãoutilizando associação da tela de titânio com PMMA.Nos três casos, os pacientes evoluíram com melhora doquadro clínico, mostrando-se satisfeitos com os resultadosestético e funcional. Considerações finais: emboraas condutas sejam diversificadas e variem entre cirurgiões,é importante o conhecimento das técnicas e dosmateriais a serem utilizados, bem como das vantagense desvantagens de cada intervenção, sendo de fundamentalimportância também a avaliação de cada casoem particular. (AU)

Objective: this study aims to present a series of three clinical cases with different approaches to the treatment of orbital floor fracture. Case reports: in the first case, the male patient was a victim of gunshot wounds, presenting diplopia, trismus, and aesthetic complaints. The treatment selected was the reconstruction of the orbital floor with an isolated titanium mesh. In the second case, the male patient was a victim of a motorcycle accident, evolving with diplopia and enophthalmos. The procedure followed was reconstruction using the association of a titanium mesh with polymethyl methacrylate (PMMA). In the third case, the male patient was the victim of an automobile accident, presenting enophthalmos, diplopia, and loss of malar projection. In this case, the reconstruction procedure was adopted using the association of a titanium mesh with PMMA. In all three cases, the patients evolved with improvement of the clinical condition, showing satisfaction with the aesthetic and functional results. Final considerations: although there are several procedures that vary among surgeons, it is important to understand the techniques and materials used, as well as the advantages and disadvantages of each intervention; it is also essential to assess each case in particular. (AU)

Conidia and blastospores of Metarhizium spp. and Beauveria bassiana s.l.: Their development during the infection process and virulence against the tick Rhipicephalus microplus.

The current study compared the virulence of conidia and blastospores of Metarhizium robertsii (IP 146), M. anisopliae sensu lato (s.l.) (IP 363) and Beauveria bassiana s.l. (IP 361 and CG 307) against unfed larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae). In addition, the development of fungal propagules on tick cuticle was investigated. Tick larvae were treated with fungal suspensions at 106, 107 or 108 propagules mL-1, and percent mortality was assessed every two days. Engorged females were immersed in fungal suspensions (1.0 × 107 propagules mL-1) for 1 min, and their biological parameters monitored daily. The virulence of conidia and blastospores against larvae varied considerably among the isolates tested. Only females treated with blastospores of IP 146 or IP 361 presented lower nutrient and egg production indices than the control group; the higher percent control was reached when females were treated with blastospores of IP 146 (98%), IP 363 (79%), or IP 361 (93%) in comparison to the groups treated with conidia, 71%, 59%, or 63%, respectively. Engorged females treated with conidia or blastospores of IP 146 or IP 361 were also examined by scanning electron microscopy (SEM). Germination of blastospores of IP 146 and IP 361 was observed on tick cuticle after 4 h incubation at 27 ±â€¯1 °C and RH > 90%, whereas germ tubes from conidia of both isolates were observed at 48 h. Appressoria in developing blastospores of B. bassiana IP 361 were observed after 4 h incubation, whereas no appressoria were seen in developing blastospores of M. robertsii IP 146. Blastospore penetrations by both fungal isolates through natural openings was also evidenced by SEM; fine sections of R. microplus engorged females treated with blastospores of IP 146 or IP 361 confirmed that these isolates penetrated through their cuticle and natural openings. Blastospores might be promising for use in biocontrol of ticks, since they are virulent against R. microplus and present rapid development on their cuticle.

Parotidectomia parcial como tratamento para adenoma pleomórfico ­ relato de caso / Partial parotidectomy as treatment for pleomorphic adenoma ­ case report

O adenoma pleomórfico é o tumor de glândula salivar mais comum, sendo caracterizado por ser uma massa de crescimento lento e indolor. A maioria dos adenomas pleomórficos de parótida estão localizados no lobo superficial da glândula, enquanto poucos ocorrem no lobo profundo. Sinais e sintomas como presença de dor, comprometimento do nervo facial e otalgia são raros e devem alertar o clínico para uma possível transformação maligna do tumor. O tratamento do adenoma pleomórfico consiste na remoção cirúrgica do tumor por enucleação extracapsular ou parotidectomia. Nesse sentido, este trabalho tem por objetivo relatar um caso clínico de adenoma pleomórfico localizado em região de glândula parótida esquerda, tratado através de parotidectomia parcial. Paciente procurou o serviço de Cirurgia de Cabeça e Pescoço do Hospital Santo Antônio das Obras Sociais Irmã Dulce queixando-se de aumento de volume em região de parótida esquerda há cerca de um ano. Após anamnese, exame físico e realização de exames complementares, chegou-se ao diagnóstico de adenoma pleomórfico. Foi realizada parotidectomia parcial à esquerda com identificação e isolamento do nervo facial, entretanto, o paciente relatou parestesia no pós-operatório, sendo esta uma complicação frequente e comumente transitória em razão da manipulação da glândula parótida. Sendo assim, a parotidectomia parcial apresenta-se como alternativa terapêutica viável para o tratamento de adenoma pleomórfico de lóbulo profundo de glândula parótida quando o tumor apresenta-se com características de benignidade e fácil acesso transcirúrgico (AU).

The pleomorphic adenoma is the most common salivary gland tumor, being characterized by a mass of slow and painless growth. Most pleomorphic adenomas are located in the superficial lobe of the gland, while a few occur in the deep lobe. Signs and symptoms such as presence of pain, facial nerve impairment and otalgia are rare and should alert the clinician to a possible malignant transformation of the tumor. Treatment of the pleomorphic adenoma consists of surgical removal by extracapsular enucleation or parotidectomy. This work aims to report a clinical case of pleomorphic adenoma located in a region of left parotid gland treated through partial parotidectomy. Patient sought the plastic surgery service of the Hospital Santo Antônio das Obras Sociais Irmã Dulce complaining of increased volume in the left parotid region for about a year. After anamnesis, physical examination and complementary exams, it was reached the diagnosis of pleomorphic adenoma. Partial left parotidectomy was performed with identification and isolation of the facial nerve and its branches, however, the patient reported postoperative paresthesia, which is a frequent and usually transient complication due to parotid gland manipulation. Therefore, partial parotidectomy is a viable therapeutic alternative for the treatment of pleomorphic adenoma of the parotid gland when the tumor presents benignity characteristics and easy transsurgical access. Patient evolves without postoperative complications, with preservation of the facial mimetic (AU).

Reconstrução de defeito em osso frontal com polimetilmetacrilato: relato de caso / Reconstruction of defect in frontal bone with polymethyl

O osso autógeno é descrito na literatura como um dos materiais mais utilizados para enxertia, no entanto, em virtude do potencial de reabsorção e da morbidade cirúrgica do sítio doador, materiais aloplásticos têm sido desenvolvidos para a mesma finalidade. Dentre os materiais aloplásticos, o polimetilmetacrilato (PMMA) se configura como um material para enxerto que permite reconstrução satisfatória de defeitos ósseos causados por traumas em face. O objetivo deste trabalho é relatar o caso de um paciente que buscou tratamento de defeito estético em osso frontal, ocasionado por trauma em face. Foi realizada cirurgia por acesso coronal e instalação de enxerto com PMMA, manipulado in ito a fim de restabelecer a morfologia do crânio, alcançando resultado satisfatório(AU)

Autogenous bone is described in the literature as the excellence graft material, however, because of its limited amount, coupled with the morbidity of the donor site, in addition to the unexpected resorption In the long run, alloplastic materials have been used. It is worth mentioning that alloplastic materials are used when there is a shortage of bone, and among them, titanium, porous polyethylene, hydroxyapatite, polymethylmethacrylate (PMMA) and others are available for dental use. The purpose of this paper is to report the case of a patient who sought the Service of Oral and Maxillofacial Surgery and Traumatology of the Federal University of Bahia for the treatment of an aesthetic defect in the frontal bone caused by facial trauma. It was proposed to perform bicoronal access surgery and graft installation with PMMA in order to restore the morphology of the skull, which was successfully achieved. PMMA is a graft material that allows a satisfactory reconstruction of the bone defects caused by trauma to the face, however, new studies must be performed considering that the market always presents innovations for the treatment of such lesions on the face(AU)

Heat-stressed Metarhizium anisopliae: viability (in vitro) and virulence (in vivo) assessments against the tick Rhipicephalus sanguineus.

The current study investigated the thermotolerance of Metarhizium anisopliae s.l. conidia from the commercial products Metarril® SP Organic and Metarril® WP. The efficacy of these M. anisopliae formulations against the tick Rhipicephalus sanguineus s.l. was studied in laboratory under optimum or heat-stress conditions. The products were prepared in water [Tween® 80, 0.01 % (v/v)] or pure mineral oil. Conidia from Metarril® SP Organic suspended in water presented markedly delayed germination after heating to constant 40 °C (for 2, 4, or 6 h) compared to conidia suspended in mineral oil. Metarril® SP Organic suspended in oil and exposed to daily cycles of heat-stress (40 °C for 4 h and 25 °C for 19 h for 5 consecutive days) presented relative germination of conidia ranging from 92.8 to 87.2 % from day 1 to day 5, respectively. Conversely, germination of conidia prepared in water ranged from 79.3 to 39.1 % from day 1 to day 5, respectively. Culturability of Metarril® WP decreased from 96 % when conidia were cultured for 30 min prior to heat exposure (40 °C for 4 h) to 9 % when conidia were cultured for 8 h. Tick percent control was distinctly higher when engorged females were treated with oil suspensions rather than water suspensions, even when treated ticks were exposed to heat-stress regimen. Oil-based applications protected fungal conidia against heat-stress. Although Metarril® is not registered for tick control, it may be useful for controlling R. sanguineus, especially if it is prepared in mineral oil.

Effect of heat stress and oil formulation on conidial germination of Metarhizium anisopliae s.s. on tick cuticle and artificial medium.

The effect of heat stress (45°C) versus non-heat stress (27°C) on germination of Metarhizium anisopliae sensu stricto (s.s.) isolate IP 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (Tween 80, 0.01%), and then applied onto the cuticle of Rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (PDAY). In addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-engorged adult R. sanguineus females. Conidia suspended in water then exposed to 45°C, in comparison to conidia formulated in mineral oil and exposed to the same temperature, germinated less and more slowly when incubated on either PDAY medium or tick cuticle. Also, conidial germination on tick cuticle was delayed in comparison to germination on artificial culture medium; for example, germination was 13% on tick cuticle 72h after inoculation, in contrast to 61.5% on PDAY medium. Unheated (27°C) conidia suspended in either water or oil and applied to tick cuticle developed appressoria 36h after treatment; whereas only heat-stressed conidia formulated in oil developed appressoria on tick cuticle. In comparison to conidia heated in mineral oil, there was a strong negative effect of heat on germination of conidia heated in water before being applied to arthropod cuticle. Nevertheless, bioassays [based primarily on egg production (quantity) and egg hatchability] exhibited high percentages of tick control regardless of the type of conidial suspension; i.e., water- or oil-formulated conidia, and whether or not conidia were previously exposed to heat. In comparison to aqueous conidial preparations, however, conidia formulated in oil reduced egg hatchability irrespective of heat or no-heat exposure. In conclusion, mineral-oil formulation protected conidia against heat-induced delay of both germination and appressorium production when applied to the cuticle of R. sanguineus.